Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0016535

Physical Location & Seq

Genomic Coordinates Reverse (-)

7346654 .. 7348437

Gene Structure 1784 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1062500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 780 bp

ATGATTGATCTGAAAAATTTACCATTATCAATTCATGTAATTTTATTATTTTATGTTCCAAACTGTGGGGTTGAGAATCAATCGGTTGGGAGTCCGGTTGTGGCTAGAATGTCGAATTTATACGGAGATTATAACCAGAAGATTGATTATGTGTTCAAAGTGGTGTTGATTGGAGATTCAGCGGTGGGGAAAACACAGCTGCTCGCACGATTTGCTAGAAATGAATTCAGTGTGGACTCGAAAGCCACGATAGGGGTCGAATTTCAAACAAAAACACTTGTTCTCGATCAGAAAACCGTCAAGGCGCAGATTTGGGACACGGCTGGCCAAGAAAGGTACAGAGCAGTCACGAGCGCATACTACCGAGGTGCAGTAGGAGCAATGTTGGTTTATGACATGACCAAGCGTCAATCGTTTGACCACATGGCTAGGTGGTTGGAGGAATTGAGGGGGCATGCCGATAAGAACATCGTTATAATGCTCATCGGCAACAAATGTGACTTGGGGAGTCTTAGAGCAGTGCCAACTGAGGATGCACAGGAGTTTGCTCAAAGAGAAAACCTATTTTTTATGGAAACATCCGCTCTTGAAGCCACCAACGTTGAAACTGCGTTTTTGATGATCTTAAGGGAGATATACCGGATAATGAGCAAGAAGACGCTTGCTGCTAGTGACCTAGACGGCGGAGATTCAGGTCTCCTAAAAGGAACAGCAATTATTGTTCCCGGTCCAGATGGGGATGGTTCTAGGAAGGGTGGGTGCTGCTTCTCCTCCACTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0001558 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0008150 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0010769 GO:0012505 GO:0016043 GO:0016049 GO:0019899 GO:0019900 GO:0022414 GO:0022603 GO:0022604 GO:0030133 GO:0030154 GO:0030427 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032989 GO:0035838 GO:0040007 GO:0040008 GO:0042995 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043900 GO:0044424 GO:0044444 GO:0044463 GO:0044464 GO:0044706 GO:0045177 GO:0045595 GO:0048468 GO:0048588 GO:0048589 GO:0048638 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0051239 GO:0051286 GO:0051510 GO:0051704 GO:0060284 GO:0060560 GO:0065007 GO:0070382 GO:0071840 GO:0080092 GO:0090404 GO:0090406 GO:0097708 GO:0099503 GO:0120025 GO:0120038 GO:2000241

KEGG Pathways

Metabolic & Signaling

ko04144 ko04961 ko04962 ko04972 map04144 map04961 map04962 map04972

Pfam Domains

Protein Families

Ras

Protein Analysis

260

Amino Acids

28.6

Weight (kDa)

6.33

Isoelectric Point (pI)

34.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Arf	PF00025	51 - 186	1.2e-13	ADP-ribosylation factor family
Ras	PF00071	53 - 213	5e-63	Ras family
Roc	PF08477	53 - 167	3e-35	Ras of Complex, Roc, domain of DAPkinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0016535)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G12160
fragaria_vesca	FvH4_6g22850
malus_domestica	MD12G1062500.v1.1 MD14G1063200.v1.1
prunus_persica	Prupe.7G071600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0480591
rosa_laevigata	RLG00000023465
rosa_multiflora	Rmu_sc0002205.1_g000059
rosa_roxburghii	Rroxscaffold_6G00401260
rosa_rugosa	Rorug03G0184300
rosa_samantha	Rh3AG236400 Rh3CG265900 Rh3DG261700
rosa_wichuraiana	Rw3G021180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	132, 476
AccB7I	CCANNNNNTGG	1 cut(s)	65
AccBSI	CCGCTC	1 cut(s)	584
AciI	CCGC	3 cut(s)	182, 582, 684
AclI	AACGTT	1 cut(s)	600
AcoI	YGGCCR	1 cut(s)	325
AcsI	RAATTY	4 cut(s)	16, 115, 224, 260
AfaI	GTAC	1 cut(s)	338
AfiI	CCNNNNNNNGG	2 cut(s)	65, 252
AflII	CTTAAG	1 cut(s)	625
AgsI	TTSAA	4 cut(s)	157, 266, 590, 605
AhdI	GACNNNNNGTC	1 cut(s)	405
AluBI	AGCT	1 cut(s)	199
AluI	AGCT	1 cut(s)	199
Alw26I	GTCTC	1 cut(s)	701
AoxI	GGCC	1 cut(s)	325
ApeKI	GCWGC	3 cut(s)	199, 665, 762
ApoI	RAATTY	4 cut(s)	16, 115, 224, 260
AspLEI	GCGC	2 cut(s)	307, 356
AspS9I	GGNCC	1 cut(s)	728
AsuC2I	CCSGG	1 cut(s)	726
AvaII	GGWCC	1 cut(s)	728
BalI	TGGCCA	1 cut(s)	327
BauI	CACGAG	1 cut(s)	349
BbsI	GAAGAC	1 cut(s)	662
BbvI	GCAGC	3 cut(s)	186, 652, 749
BccI	CCATC	2 cut(s)	728, 734
BceAI	ACGGC	2 cut(s)	336, 697
BcnI	CCSGG	1 cut(s)	726
BcoDI	GTCTC	1 cut(s)	701
BfaI	CTAG	6 cut(s)	105, 216, 429, 669, 677, 747
BfrI	CTTAAG	1 cut(s)	625
BisI	GCNGC	3 cut(s)	200, 666, 763
BlsI	GCNGC	3 cut(s)	201, 667, 764
Bme1390I	CCNGG	1 cut(s)	726
Bme18I	GGWCC	1 cut(s)	728
BmeRI	GACNNNNNGTC	1 cut(s)	405
BmgT120I	GGNCC	1 cut(s)	728
BmrFI	CCNGG	1 cut(s)	726
BmsI	GCATC	1 cut(s)	523
BpiI	GAAGAC	1 cut(s)	662
BpuMI	CCSGG	1 cut(s)	726
BsaI	GGTCTC	1 cut(s)	701
BsaJI	CCNNGG	1 cut(s)	364
BsaWI	WCCGGW	2 cut(s)	94, 639
Bsc4I	CCNNNNNNNGG	2 cut(s)	65, 252
Bse3DI	GCAATG	1 cut(s)	387
BseDI	CCNNGG	1 cut(s)	364
BseGI	GGATG	3 cut(s)	538, 578, 745
BseLI	CCNNNNNNNGG	2 cut(s)	65, 252
BseMI	GCAATG	1 cut(s)	387
BseMII	CTCAG	1 cut(s)	519
BseRI	GAGGAG	1 cut(s)	760
BseXI	GCAGC	3 cut(s)	186, 652, 749
BsgI	GTGCAG	1 cut(s)	390
BshFI	GGCC	1 cut(s)	327
BsiSI	CCGG	3 cut(s)	95, 640, 726
BslFI	GGGAC	1 cut(s)	329
BslI	CCNNNNNNNGG	2 cut(s)	65, 252
BsmAI	GTCTC	1 cut(s)	701
BsmFI	GGGAC	1 cut(s)	329
BsnI	GGCC	1 cut(s)	327
Bso31I	GGTCTC	1 cut(s)	701
Bsp143I	GATC	3 cut(s)	7, 286, 621
BspACI	CCGC	3 cut(s)	182, 582, 684
BspANI	GGCC	1 cut(s)	327
BspCNI	CTCAG	1 cut(s)	520
BspTI	CTTAAG	1 cut(s)	625
BspTNI	GGTCTC	1 cut(s)	701
BsrBI	CCGCTC	1 cut(s)	584
BsrDI	GCAATG	1 cut(s)	387
BssECI	CCNNGG	1 cut(s)	364
BssMI	GATC	3 cut(s)	7, 286, 621
BssSI	CACGAG	1 cut(s)	349
Bst2BI	CACGAG	1 cut(s)	349
Bst4CI	ACNGT	2 cut(s)	65, 298
BstAFI	CTTAAG	1 cut(s)	625
BstAPI	GCANNNNNTGC	1 cut(s)	212
BstC8I	GCNNGC	4 cut(s)	204, 325, 456, 663
BstDEI	CTNAG	2 cut(s)	512, 528
BstF5I	GGATG	3 cut(s)	538, 578, 745
BstHHI	GCGC	2 cut(s)	307, 356
BstKTI	GATC	3 cut(s)	10, 289, 624
BstMAI	GTCTC	1 cut(s)	701
BstMBI	GATC	3 cut(s)	7, 286, 621
BstMWI	GCNNNNNNNGC	3 cut(s)	212, 377, 590
BstNSI	RCATGY	1 cut(s)	458
BstSCI	CCNGG	1 cut(s)	724
BstV1I	GCAGC	3 cut(s)	186, 652, 749
BstV2I	GAAGAC	1 cut(s)	662
BsuRI	GGCC	1 cut(s)	327
BtsCI	GGATG	3 cut(s)	538, 578, 745
BtsI	GCAGTG	1 cut(s)	525
BtsIMutI	CAGTG	2 cut(s)	235, 525
Cac8I	GCNNGC	4 cut(s)	204, 325, 456, 663
CfoI	GCGC	2 cut(s)	307, 356
Cfr13I	GGNCC	1 cut(s)	728
CseI	GACGC	2 cut(s)	395, 667
Csp6I	GTAC	1 cut(s)	337
CspCI	CAANNNNNGTGG	2 cut(s)	583, 618
CviAII	CATG	4 cut(s)	35, 397, 424, 455
CviJI	RGCY	7 cut(s)	104, 199, 245, 323, 327, 428, 593
CviKI_1	RGCY	7 cut(s)	104, 199, 245, 323, 327, 428, 593
CviQI	GTAC	1 cut(s)	337
DdeI	CTNAG	2 cut(s)	512, 528
DpnI	GATC	3 cut(s)	9, 288, 623
DpnII	GATC	3 cut(s)	7, 286, 621
DriI	GACNNNNNGTC	1 cut(s)	405
EaeI	YGGCCR	1 cut(s)	325
Eam1105I	GACNNNNNGTC	1 cut(s)	405
EciI	GGCGGA	1 cut(s)	699
Eco31I	GGTCTC	1 cut(s)	701
Eco47I	GGWCC	1 cut(s)	728
EcoRI	GAATTC	1 cut(s)	224
FaeI	CATG	4 cut(s)	38, 400, 427, 458
FaqI	GGGAC	1 cut(s)	329
FatI	CATG	4 cut(s)	34, 396, 423, 454
Fnu4HI	GCNGC	3 cut(s)	200, 666, 763
FokI	GGATG	3 cut(s)	545, 565, 752
Fsp4HI	GCNGC	3 cut(s)	200, 666, 763
FspBI	CTAG	6 cut(s)	105, 216, 429, 669, 677, 747
GlaI	GCGC	2 cut(s)	306, 355
GluI	GCNGC	3 cut(s)	200, 666, 763
HaeIII	GGCC	1 cut(s)	327
HapII	CCGG	3 cut(s)	95, 640, 726
HgaI	GACGC	2 cut(s)	395, 667
HhaI	GCGC	2 cut(s)	307, 356
Hin1II	CATG	4 cut(s)	38, 400, 427, 458
Hin6I	GCGC	2 cut(s)	305, 354
HinP1I	GCGC	2 cut(s)	305, 354
HinfI	GANTC	6 cut(s)	76, 91, 176, 236, 508, 689
HpaII	CCGG	3 cut(s)	95, 640, 726
Hpy166II	GTNNAC	1 cut(s)	235
Hpy188I	TCNGA	2 cut(s)	12, 291
Hpy188III	TCNNGA	4 cut(s)	284, 349, 587, 731
Hpy8I	GTNNAC	1 cut(s)	235
HpyAV	CCTTC	1 cut(s)	745
HpyCH4III	ACNGT	2 cut(s)	65, 298
HpyCH4IV	ACGT	1 cut(s)	600
HpyCH4V	TGCA	2 cut(s)	371, 536
HpyF10VI	GCNNNNNNNGC	3 cut(s)	212, 377, 590
HpyF3I	CTNAG	2 cut(s)	512, 528
HpySE526I	ACGT	1 cut(s)	600
Hsp92II	CATG	4 cut(s)	38, 400, 427, 458
HspAI	GCGC	2 cut(s)	305, 354
Kzo9I	GATC	3 cut(s)	7, 286, 621
LmnI	GCTCC	1 cut(s)	377
LpnPI	CCDG	8 cut(s)	108, 149, 309, 524, 653, 678, 739, 744
Lsp1109I	GCAGC	3 cut(s)	186, 652, 749
LweI	GCATC	1 cut(s)	523
MaeI	CTAG	6 cut(s)	105, 216, 429, 669, 677, 747
MaeII	ACGT	1 cut(s)	600
MaeIII	GTNAC	3 cut(s)	346, 497, 671
MalI	GATC	3 cut(s)	9, 288, 623
MbiI	CCGCTC	1 cut(s)	584
MboI	GATC	3 cut(s)	7, 286, 621
MboII	GAAGA	2 cut(s)	151, 667
MlsI	TGGCCA	1 cut(s)	327
MluCI	AATT	8 cut(s)	16, 30, 39, 115, 224, 260, 443, 714
MluNI	TGGCCA	1 cut(s)	327
MlyI	GAGTC	3 cut(s)	100, 230, 517
MmeI	TCCRAC	1 cut(s)	417
MnlI	CCTC	4 cut(s)	359, 433, 441, 523
Mox20I	TGGCCA	1 cut(s)	327
MscI	TGGCCA	1 cut(s)	327
MseI	TTAA	2 cut(s)	626, 778
Msp20I	TGGCCA	1 cut(s)	327
MspA1I	CMGCKG	2 cut(s)	182, 199
MspCI	CTTAAG	1 cut(s)	625
MspI	CCGG	3 cut(s)	95, 640, 726
MspR9I	CCNGG	1 cut(s)	726
MwoI	GCNNNNNNNGC	3 cut(s)	212, 377, 590
NciI	CCSGG	1 cut(s)	726
NdeII	GATC	3 cut(s)	7, 286, 621
NlaIII	CATG	4 cut(s)	38, 400, 427, 458
NmuCI	GTSAC	3 cut(s)	346, 497, 671
NspI	RCATGY	1 cut(s)	458
PaeI	GCATGC	1 cut(s)	458
PcsI	WCGNNNNNNNCGW	1 cut(s)	245
PfeI	GAWTC	3 cut(s)	76, 176, 689
PflMI	CCANNNNNTGG	1 cut(s)	65
PkrI	GCNGC	3 cut(s)	201, 667, 764
PleI	GAGTC	3 cut(s)	99, 230, 516
PpsI	GAGTC	3 cut(s)	99, 230, 516
PsiI	TTATAA	2 cut(s)	132, 476
Psp1406I	AACGTT	1 cut(s)	600
PspPI	GGNCC	1 cut(s)	728
PvuII	CAGCTG	1 cut(s)	199
RsaI	GTAC	1 cut(s)	338
RsaNI	GTAC	1 cut(s)	337
SaqAI	TTAA	2 cut(s)	626, 778
SatI	GCNGC	3 cut(s)	200, 666, 763
Sau3AI	GATC	3 cut(s)	7, 286, 621
Sau96I	GGNCC	1 cut(s)	728
SchI	GAGTC	3 cut(s)	100, 230, 517
ScrFI	CCNGG	1 cut(s)	726
SetI	ASST	8 cut(s)	201, 338, 370, 434, 564, 603, 678, 697
SfaNI	GCATC	1 cut(s)	523
SinI	GGWCC	1 cut(s)	728
SmlI	CTYRAG	1 cut(s)	625
SmoI	CTYRAG	1 cut(s)	625
SphI	GCATGC	1 cut(s)	458
Sse9I	AATT	8 cut(s)	16, 30, 39, 115, 224, 260, 443, 714
SsiI	CCGC	3 cut(s)	182, 582, 684
SspMI	CTAG	6 cut(s)	105, 216, 429, 669, 677, 747
StyD4I	CCNGG	1 cut(s)	724
TaaI	ACNGT	2 cut(s)	65, 298
TaiI	ACGT	1 cut(s)	603
TaqI	TCGA	4 cut(s)	113, 239, 258, 285
TasI	AATT	8 cut(s)	16, 30, 39, 115, 224, 260, 443, 714
TfiI	GAWTC	3 cut(s)	76, 176, 689
Tru1I	TTAA	2 cut(s)	626, 778
Tru9I	TTAA	2 cut(s)	626, 778
TscAI	CASTG	2 cut(s)	235, 525
TseFI	GTSAC	3 cut(s)	346, 497, 671
TseI	GCWGC	3 cut(s)	199, 665, 762
Tsp45I	GTSAC	3 cut(s)	346, 497, 671
TspDTI	ATGAA	2 cut(s)	23, 237
TspGWI	ACGGA	1 cut(s)	138
TspRI	CASTG	2 cut(s)	235, 525
Van91I	CCANNNNNTGG	1 cut(s)	65
Vha464I	CTTAAG	1 cut(s)	625
VpaK11BI	GGWCC	1 cut(s)	728
XapI	RAATTY	4 cut(s)	16, 115, 224, 260
XceI	RCATGY	1 cut(s)	458
XspI	CTAG	6 cut(s)	105, 216, 429, 669, 677, 747

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.