Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0016535

Physical Location & Seq

Genomic Coordinates Reverse (-)

6689959 .. 6691548

Gene Structure 1590 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1063200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 780 bp

ATGATTGATCTGAGAAGTTTACCATCATTATTTCATGTAATTTTGGTATTTTATGTTCCAGATTGTGGGGTTGAGAATCAATCGGTTTGGGGTCCGGTCGCGGCGAGGATGTCGAATTTATACGGAGATTACAATCAGAAGATAGATTATGTGTTCAAAGTGGTGTTGATTGGAGACTCGGCGGTGGGGAAAACACAGCTGCTCGCGCGGTTTGCTAGGAATGAATTCAGTGTGGACTCAAAAGCCACTATCGGTGTCGAATTTCAGACGAAAACCCTTGTTCTCGATCACAAGACCGTCAAGGCGCAAATTTGGGACACGGCTGGCCAAGAAAGGTACAGAGCAGTCACGAGCGCATACTACCGGGGAGCAGTAGGAGCAATGTTGGTTTATGATATGACTAAGCGTCAATCGTTTGATCACATGGCACGGTGGTTGGAGGAATTGAGGGGGCATGCTGATAAGAACATTGTTATAATGCTCATCGGCAACAAATGTGACTTGGGTAGTCTTCGAGCAGTGCCAATTGAGGATGCACAGGAGTTTGCTCAAAGAGAAAACCTATTTTTTATGGAAACATCTGCTCTTGAGGCCACCAACGTGGAAACCGCATTTTTGATGATCTTAATGGAGATATACCGGATAATGAGCAAGAAGCCGCTTGCTGCTGGTGACCTAAACAGTGGAGATTCAGGGCTTCTAAAAGGAACAACAATTATTGTTCCCGGCCCGGATGGGGATGGTGCTAGGAAGGGTGGCTGCTGCTTCTCCTCCACCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0001558 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0008150 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0010769 GO:0012505 GO:0016043 GO:0016049 GO:0019899 GO:0019900 GO:0022414 GO:0022603 GO:0022604 GO:0030133 GO:0030154 GO:0030427 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032989 GO:0035838 GO:0040007 GO:0040008 GO:0042995 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043900 GO:0044424 GO:0044444 GO:0044463 GO:0044464 GO:0044706 GO:0045177 GO:0045595 GO:0048468 GO:0048588 GO:0048589 GO:0048638 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0051239 GO:0051286 GO:0051510 GO:0051704 GO:0060284 GO:0060560 GO:0065007 GO:0070382 GO:0071840 GO:0080092 GO:0090404 GO:0090406 GO:0097708 GO:0099503 GO:0120025 GO:0120038 GO:2000241

KEGG Pathways

Metabolic & Signaling

ko04144 ko04961 ko04962 ko04972 map04144 map04961 map04962 map04972

Pfam Domains

Protein Families

Ras

Protein Analysis

260

Amino Acids

28.7

Weight (kDa)

6.11

Isoelectric Point (pI)

34.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Arf	PF00025	51 - 173	9.6e-14	ADP-ribosylation factor family
Ras	PF00071	53 - 213	5.1e-62	Ras family
Roc	PF08477	53 - 167	4.2e-35	Ras of Complex, Roc, domain of DAPkinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016535)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G12160
fragaria_vesca	FvH4_6g22850
malus_domestica	MD12G1062500.v1.1 MD14G1063200.v1.1
prunus_persica	Prupe.7G071600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0480591
rosa_laevigata	RLG00000023465
rosa_multiflora	Rmu_sc0002205.1_g000059
rosa_roxburghii	Rroxscaffold_6G00401260
rosa_rugosa	Rorug03G0184300
rosa_samantha	Rh3AG236400 Rh3CG265900 Rh3DG261700
rosa_wichuraiana	Rw3G021180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	476
AccB7I	CCANNNNNTGG	1 cut(s)	65
AccII	CGCG	3 cut(s)	101, 206, 208
AciI	CCGC	5 cut(s)	101, 182, 208, 609, 659
AcoI	YGGCCR	1 cut(s)	325
AcsI	RAATTY	4 cut(s)	115, 224, 260, 309
AfaI	GTAC	1 cut(s)	338
AfiI	CCNNNNNNNGG	2 cut(s)	65, 736
AgsI	TTSAA	1 cut(s)	157
AhdI	GACNNNNNGTC	1 cut(s)	405
AleI	CACNNNNGTG	1 cut(s)	599
AluBI	AGCT	1 cut(s)	199
AluI	AGCT	1 cut(s)	199
Alw26I	GTCTC	1 cut(s)	168
AoxI	GGCC	3 cut(s)	325, 591, 727
ApeKI	GCWGC	4 cut(s)	199, 665, 759, 762
ApoI	RAATTY	4 cut(s)	115, 224, 260, 309
Asp700I	GAANNNNTTC	1 cut(s)	224
AspLEI	GCGC	3 cut(s)	208, 307, 356
AspS9I	GGNCC	2 cut(s)	92, 728
AsuC2I	CCSGG	3 cut(s)	365, 726, 731
AsuHPI	GGTGA	1 cut(s)	683
AvaII	GGWCC	1 cut(s)	92
BalI	TGGCCA	1 cut(s)	327
BauI	CACGAG	1 cut(s)	349
BbsI	GAAGAC	1 cut(s)	503
BbvI	GCAGC	4 cut(s)	186, 652, 746, 749
BccI	CCATC	3 cut(s)	31, 728, 734
BceAI	ACGGC	1 cut(s)	336
BclI	TGATCA	1 cut(s)	418
BcnI	CCSGG	3 cut(s)	365, 726, 731
BcoDI	GTCTC	1 cut(s)	168
BfaI	CTAG	2 cut(s)	216, 747
BisI	GCNGC	6 cut(s)	102, 200, 659, 666, 760, 763
BlsI	GCNGC	6 cut(s)	103, 201, 660, 667, 761, 764
Bme1390I	CCNGG	3 cut(s)	365, 726, 731
Bme18I	GGWCC	1 cut(s)	92
BmeRI	GACNNNNNGTC	1 cut(s)	405
BmgT120I	GGNCC	2 cut(s)	92, 728
BmiI	GGNNCC	1 cut(s)	93
BmrFI	CCNGG	3 cut(s)	365, 726, 731
BmsI	GCATC	1 cut(s)	523
BpiI	GAAGAC	1 cut(s)	503
BpuEI	CTTGAG	1 cut(s)	608
BpuMI	CCSGG	3 cut(s)	365, 726, 731
BsaBI	GATNNNNATC	1 cut(s)	132
BsaJI	CCNNGG	1 cut(s)	364
BsaWI	WCCGGW	2 cut(s)	94, 639
Bsc4I	CCNNNNNNNGG	2 cut(s)	65, 736
Bse3DI	GCAATG	1 cut(s)	387
Bse8I	GATNNNNATC	1 cut(s)	132
BseDI	CCNNGG	1 cut(s)	364
BseGI	GGATG	4 cut(s)	114, 538, 739, 745
BseJI	GATNNNNATC	1 cut(s)	132
BseLI	CCNNNNNNNGG	2 cut(s)	65, 736
BseMI	GCAATG	1 cut(s)	387
BseRI	GAGGAG	1 cut(s)	760
BseXI	GCAGC	4 cut(s)	186, 652, 746, 749
Bsh1236I	CGCG	3 cut(s)	101, 206, 208
Bsh1285I	CGRYCG	1 cut(s)	99
BshFI	GGCC	3 cut(s)	327, 593, 729
BsiEI	CGRYCG	1 cut(s)	99
BsiSI	CCGG	5 cut(s)	95, 364, 640, 726, 731
BslFI	GGGAC	1 cut(s)	329
BslI	CCNNNNNNNGG	2 cut(s)	65, 736
BsmAI	GTCTC	1 cut(s)	168
BsmFI	GGGAC	1 cut(s)	329
BsnI	GGCC	3 cut(s)	327, 593, 729
Bsp143I	GATC	4 cut(s)	7, 286, 418, 621
BspACI	CCGC	5 cut(s)	101, 182, 208, 609, 659
BspANI	GGCC	3 cut(s)	327, 593, 729
BspFNI	CGCG	3 cut(s)	101, 206, 208
BspLI	GGNNCC	1 cut(s)	93
BsrDI	GCAATG	1 cut(s)	387
BssECI	CCNNGG	1 cut(s)	364
BssMI	GATC	4 cut(s)	7, 286, 418, 621
BssSI	CACGAG	1 cut(s)	349
Bst2BI	CACGAG	1 cut(s)	349
Bst4CI	ACNGT	3 cut(s)	298, 432, 683
BstC8I	GCNNGC	4 cut(s)	204, 325, 456, 663
BstDEI	CTNAG	2 cut(s)	11, 402
BstEII	GGTNACC	1 cut(s)	671
BstF5I	GGATG	4 cut(s)	114, 538, 739, 745
BstFNI	CGCG	3 cut(s)	101, 206, 208
BstHHI	GCGC	3 cut(s)	208, 307, 356
BstKTI	GATC	4 cut(s)	10, 289, 421, 624
BstMAI	GTCTC	1 cut(s)	168
BstMBI	GATC	4 cut(s)	7, 286, 418, 621
BstMCI	CGRYCG	1 cut(s)	99
BstMWI	GCNNNNNNNGC	4 cut(s)	205, 212, 377, 590
BstNSI	RCATGY	1 cut(s)	458
BstPI	GGTNACC	1 cut(s)	671
BstSCI	CCNGG	3 cut(s)	363, 724, 729
BstUI	CGCG	3 cut(s)	101, 206, 208
BstV1I	GCAGC	4 cut(s)	186, 652, 746, 749
BstV2I	GAAGAC	1 cut(s)	503
BstXI	CCANNNNNNTGG	1 cut(s)	601
BsuRI	GGCC	3 cut(s)	327, 593, 729
BtsCI	GGATG	4 cut(s)	114, 538, 739, 745
BtsI	GCAGTG	1 cut(s)	525
BtsIMutI	CAGTG	3 cut(s)	235, 525, 688
Cac8I	GCNNGC	4 cut(s)	204, 325, 456, 663
CfoI	GCGC	3 cut(s)	208, 307, 356
Cfr13I	GGNCC	2 cut(s)	92, 728
CseI	GACGC	1 cut(s)	395
Csp6I	GTAC	1 cut(s)	337
CviAII	CATG	3 cut(s)	35, 424, 455
CviJI	RGCY	9 cut(s)	199, 245, 323, 327, 593, 658, 697, 729, 759
CviKI_1	RGCY	9 cut(s)	199, 245, 323, 327, 593, 658, 697, 729, 759
CviQI	GTAC	1 cut(s)	337
DdeI	CTNAG	2 cut(s)	11, 402
DpnI	GATC	4 cut(s)	9, 288, 420, 623
DpnII	GATC	4 cut(s)	7, 286, 418, 621
DriI	GACNNNNNGTC	1 cut(s)	405
EaeI	YGGCCR	1 cut(s)	325
Eam1105I	GACNNNNNGTC	1 cut(s)	405
Eco47I	GGWCC	1 cut(s)	92
Eco91I	GGTNACC	1 cut(s)	671
EcoO65I	GGTNACC	1 cut(s)	671
EcoRI	GAATTC	1 cut(s)	224
FaeI	CATG	3 cut(s)	38, 427, 458
FaqI	GGGAC	1 cut(s)	329
FatI	CATG	3 cut(s)	34, 423, 454
FbaI	TGATCA	1 cut(s)	418
Fnu4HI	GCNGC	6 cut(s)	102, 200, 659, 666, 760, 763
FokI	GGATG	4 cut(s)	121, 545, 746, 752
Fsp4HI	GCNGC	6 cut(s)	102, 200, 659, 666, 760, 763
FspBI	CTAG	2 cut(s)	216, 747
GlaI	GCGC	3 cut(s)	207, 306, 355
GluI	GCNGC	6 cut(s)	102, 200, 659, 666, 760, 763
HaeIII	GGCC	3 cut(s)	327, 593, 729
HapII	CCGG	5 cut(s)	95, 364, 640, 726, 731
HgaI	GACGC	1 cut(s)	395
HhaI	GCGC	3 cut(s)	208, 307, 356
Hin1II	CATG	3 cut(s)	38, 427, 458
Hin6I	GCGC	3 cut(s)	206, 305, 354
HinP1I	GCGC	3 cut(s)	206, 305, 354
HinfI	GANTC	4 cut(s)	76, 176, 236, 689
HpaII	CCGG	5 cut(s)	95, 364, 640, 726, 731
HphI	GGTGA	1 cut(s)	683
Hpy166II	GTNNAC	2 cut(s)	20, 235
Hpy188I	TCNGA	3 cut(s)	12, 138, 267
Hpy188III	TCNNGA	4 cut(s)	59, 284, 349, 587
Hpy8I	GTNNAC	2 cut(s)	20, 235
HpyAV	CCTTC	1 cut(s)	745
HpyCH4III	ACNGT	3 cut(s)	298, 432, 683
HpyCH4IV	ACGT	1 cut(s)	600
HpyCH4V	TGCA	1 cut(s)	536
HpyF10VI	GCNNNNNNNGC	4 cut(s)	205, 212, 377, 590
HpyF3I	CTNAG	2 cut(s)	11, 402
HpySE526I	ACGT	1 cut(s)	600
Hsp92II	CATG	3 cut(s)	38, 427, 458
HspAI	GCGC	3 cut(s)	206, 305, 354
Ksp22I	TGATCA	1 cut(s)	418
Kzo9I	GATC	4 cut(s)	7, 286, 418, 621
LmnI	GCTCC	2 cut(s)	368, 377
Lsp1109I	GCAGC	4 cut(s)	186, 652, 746, 749
LweI	GCATC	1 cut(s)	523
MaeI	CTAG	2 cut(s)	216, 747
MaeII	ACGT	1 cut(s)	600
MaeIII	GTNAC	3 cut(s)	346, 497, 671
MalI	GATC	4 cut(s)	9, 288, 420, 623
MboI	GATC	4 cut(s)	7, 286, 418, 621
MboII	GAAGA	2 cut(s)	151, 503
MfeI	CAATTG	1 cut(s)	525
MlsI	TGGCCA	1 cut(s)	327
MluCI	AATT	8 cut(s)	39, 115, 224, 260, 309, 443, 525, 714
MluNI	TGGCCA	1 cut(s)	327
MlyI	GAGTC	2 cut(s)	170, 230
MmeI	TCCRAC	1 cut(s)	417
MnlI	CCTC	5 cut(s)	99, 433, 441, 523, 583
Mox20I	TGGCCA	1 cut(s)	327
MroXI	GAANNNNTTC	1 cut(s)	224
MscI	TGGCCA	1 cut(s)	327
MseI	TTAA	1 cut(s)	626
MslI	CAYNNNNRTG	1 cut(s)	599
Msp20I	TGGCCA	1 cut(s)	327
MspA1I	CMGCKG	1 cut(s)	199
MspI	CCGG	5 cut(s)	95, 364, 640, 726, 731
MspR9I	CCNGG	3 cut(s)	365, 726, 731
MunI	CAATTG	1 cut(s)	525
MvnI	CGCG	3 cut(s)	101, 206, 208
MwoI	GCNNNNNNNGC	4 cut(s)	205, 212, 377, 590
NciI	CCSGG	3 cut(s)	365, 726, 731
NdeII	GATC	4 cut(s)	7, 286, 418, 621
NlaIII	CATG	3 cut(s)	38, 427, 458
NlaIV	GGNNCC	1 cut(s)	93
NmeAIII	GCCGAG	1 cut(s)	158
NmuCI	GTSAC	3 cut(s)	346, 497, 671
NspI	RCATGY	1 cut(s)	458
OliI	CACNNNNGTG	1 cut(s)	599
PaeI	GCATGC	1 cut(s)	458
PdmI	GAANNNNTTC	1 cut(s)	224
PfeI	GAWTC	2 cut(s)	76, 689
PflMI	CCANNNNNTGG	1 cut(s)	65
PkrI	GCNGC	6 cut(s)	103, 201, 660, 667, 761, 764
PleI	GAGTC	2 cut(s)	170, 230
PpsI	GAGTC	2 cut(s)	170, 230
PsiI	TTATAA	1 cut(s)	476
PspEI	GGTNACC	1 cut(s)	671
PspN4I	GGNNCC	1 cut(s)	93
PspPI	GGNCC	2 cut(s)	92, 728
PsrI	GAACNNNNNNTAC	2 cut(s)	39, 71
PvuII	CAGCTG	1 cut(s)	199
RsaI	GTAC	1 cut(s)	338
RsaNI	GTAC	1 cut(s)	337
RseI	CAYNNNNRTG	1 cut(s)	599
SaqAI	TTAA	1 cut(s)	626
SatI	GCNGC	6 cut(s)	102, 200, 659, 666, 760, 763
Sau3AI	GATC	4 cut(s)	7, 286, 418, 621
Sau96I	GGNCC	2 cut(s)	92, 728
SchI	GAGTC	2 cut(s)	170, 230
ScrFI	CCNGG	3 cut(s)	365, 726, 731
SetI	ASST	6 cut(s)	201, 338, 564, 603, 678, 779
SfaNI	GCATC	1 cut(s)	523
SinI	GGWCC	1 cut(s)	92
SmiMI	CAYNNNNRTG	1 cut(s)	599
SmlI	CTYRAG	1 cut(s)	587
SmoI	CTYRAG	1 cut(s)	587
SphI	GCATGC	1 cut(s)	458
Sse9I	AATT	8 cut(s)	39, 115, 224, 260, 309, 443, 525, 714
SsiI	CCGC	5 cut(s)	101, 182, 208, 609, 659
SspMI	CTAG	2 cut(s)	216, 747
StyD4I	CCNGG	3 cut(s)	363, 724, 729
TaaI	ACNGT	3 cut(s)	298, 432, 683
TaiI	ACGT	1 cut(s)	603
TaqI	TCGA	4 cut(s)	113, 258, 285, 514
TasI	AATT	8 cut(s)	39, 115, 224, 260, 309, 443, 525, 714
TauI	GCSGC	2 cut(s)	104, 661
TfiI	GAWTC	2 cut(s)	76, 689
Tru1I	TTAA	1 cut(s)	626
Tru9I	TTAA	1 cut(s)	626
TscAI	CASTG	3 cut(s)	235, 525, 688
TseFI	GTSAC	3 cut(s)	346, 497, 671
TseI	GCWGC	4 cut(s)	199, 665, 759, 762
Tsp45I	GTSAC	3 cut(s)	346, 497, 671
TspDTI	ATGAA	2 cut(s)	23, 237
TspGWI	ACGGA	1 cut(s)	138
TspRI	CASTG	3 cut(s)	235, 525, 688
Van91I	CCANNNNNTGG	1 cut(s)	65
VpaK11BI	GGWCC	1 cut(s)	92
XapI	RAATTY	4 cut(s)	115, 224, 260, 309
XceI	RCATGY	1 cut(s)	458
XmnI	GAANNNNTTC	1 cut(s)	224
XspI	CTAG	2 cut(s)	216, 747

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.