MD12G1065000.v1.1
NAC Family

Probably inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0006756
Physical Location & Seq
Reverse (-)
7617337 .. 7620957
3621 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1065000.v1.1.491

Sequence Viewer

Length: 2616 bp
ATGGCTGCTTCCTGCAGATATCCATTGCTTCTCTCTCTAACAATCTCCATGTTTTTCCTTCTTACTTCAGCATTGTTATCTGAACCAGACATTCTTCTCACATTCAAGGCCTCCATTAAAGACCCCCAAAATTCTCTCTCAAGCTGGTCCAACACCTCCGCCACCCACCACTGCCAATGGGCTGGAATCACCTGCACCGCCGCCACCACTACCACCACCACCACCACCACCGCCACACTTTCTGTCACCTCCCTCAACCTCCAAAGCTTCAACCTTTCAGGTGACATTTCACCTTCAATCTGTGGGCTCCCCTATCTGTCAGAGCTCAACCTTGCTGACAACTTCTTCAACCAGCCCATCCCTCTCCACCTCTCTCAGTGCACTTCTCTGGAGACTTTGAATCTCAGCAACAATTTAATCTGGGGTACACTTTTAAACCAGATTTCTCAGTTCGGTAACTTGAAAGCTCTTGACTTGAGCATAAACCATGTTGAAGGAAGCATCCCACAAGGCCTTGGTGCACTCCACAACCTCCAAGTTCTCAACTTGGGTAGCAACTTGCTTTCGGGTAGTGTGCCTTCTATTTTTGGGAATTTGACTGAGCTAGTTGTTCTTGATCTGTCTCAAAATACTTACTTGGTGAGTGAGATTCCCACCGACATTGGGAAGCTTGTGAAGCTTGAGAAGTTTTTGAGTGTTTTGGACTTTTCCCAAAATAACTTAACAGGTAGGGTTCCTCAGATGCTAGGGTTTTCTCTCAAGAACTTGGTGTCTTTTGATGTTTCAGAGAATAGGCTTTCTGGGTTTTTCCCAAATGGCATCTGTGGTGGAAAGTACCTTATAAACCTCAGCCTTCACACAAATGTGTTCAATGGTACAATACCTGACTCCATTAGTGAATGCTTAAGGCTTGAGGTGTTTGAAGTTCAGAACAATTTGTTTTCTGGGGATTTCCCAGTTGGGTTATGGTCATTGCCCAAAATTAAGCTCTTGAGAGCTGAGAATAATCGGTTTTCGGGTGAAATACCTGATTCGGTATCAATGGCTGTTCAATTGGAGCAGGTTCAAATAGATAACAACAACTTCACTAGCAAAATTCCTCATGGTCTTGGGCTAGTCAAGAGCCTGTATAGATTCTCTGCTTCTCTTAATGGTTTATATGGTGAGCTCCCTACAAATTTCTGTGACTCCCCTGTTATGAGTATAATGAATCTCTCCCACAATTCGCTTTCGGGTCAAATTCCGGAGGTTAAAAAATGCAGGAAGCTAGTGTCACTGTCTTTGGCAGACAACAGTCTTAGTGGGAATATCCCACAATCCCTTGGGGAATTACCAGTGCTAACTTACCTTGATCTTTCGGATAATAAGCTTACTGGTCCCCTGCCACAGGCGCTTCAGAACTTGAAGCTTGCACTCTTTAATGTTTCCTTTAATCAGCTATCTGGTAGAGTTCCCTACTCTCTTATTGCAGGACTCCCAGCTTCATTTCTTCAAGGAAATCCTGACCTTTGCGGTTCAGGATTACTCGATTCTTGTTCTGATGACCAGCCAAGACACCATTCTTTTGATTTTACAACCTTGACATGTGTCTTGGTATCTATAGCTTTTGCTGTTGGGACTCTCATTATCACTGGTGGGTTTATCTTTTATAATCGATATTATAAGCAGAGAGCTCAAGTTGGCCTTTGGCGTTCGGTGTTCTTTTATCCTCTCAGAGTCACTGAGCATGACTTGATGATGGGAATGGATGAGAAAACTGCTGCAGGGTGTGGTGGAGTATTTGGTAGAGTTTACATTGTAAGTTTACCAAGTAATGAACTTGTTGCTGTGAAGAAGCTTGTGAATTTTGGGATCCAGTCTTCGAAAGCTTTGAAGGCTGAGATCAAGACCTTGGCCAAGATCAGGCATAAGCACATTGTAAAAGTTCTGGGATTTTGCTATTGCGATGATTCGATATTTCTGATATATGAATTCCAACAGAAAGGGAGCTTGGGGGATTTGATCTGCAGACCTGATTTTAACTTGCAGTGGAGTGTTAGATTGAGAATTGCCATTGGCATTGCGCAAGGACTGGCATACCTTCACAAAGATTATGTTCCTCATTTGCTTCATAGAAATCTCAAGTCAAACAATATCCTTCTGGACGCAGAGTTTGAACCAAAGCTCACAGATTTTGCTCTTGACAGAATTGTGGGAGAAGCTGCCTTTCAGTTAACCATGGCTTCGGAATCTGCTCTATCCTGTTACAATGCGCCAGAAAGCAAGTACAATAAGAAAGCAACTGAACAAATGGATGTTTACAGCTTTGGTGTTGTACTACTGGAGCTAGTGACAGGCCGGCCAGCAGAGCAAGCAGAGCCATCTAAATCTCTTGACATTGTAAAGTGGGTGCGACGAAAGGTCAACATTACAAATGGGGCTGTCCAAGTTCTTGATCCCAAGATCGGAAATTCTTCCCAGCAAGAGATACTAGGAGCTTTAGAAATCGCTCTCCGTTGCACTTCTGTGATGCCGGAGAAAAGACCATCAATGTCTGAAGTTGTGAGATCACTTCAGTCCCTTGACTCGAAGACCCACGAGGCAGTTATCGACTTCTCTACCTTTGAGGAGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

872

Amino Acids

95.64

Weight (kDa)

6.16

Isoelectric Point (pI)

36.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 27 - 66 2.1e-11 Leucine rich repeat N-terminal domain
LRR_14 PF23598 123 - 231 3.2e-08 Leucine-rich repeat region
LRR_8 PF13855 153 - 210 1.5e-06 Leucine rich repeat
LRR_8 PF13855 421 - 480 5.5e-06 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 592 - 851 5.4e-37 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 592 - 849 1.9e-34 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 842, 1650, 1662
AarI CACCTGC 1 cut(s) 200
Acc16I TGCGCA 1 cut(s) 2064
Acc36I ACCTGC 2 cut(s) 200, 1051
AccIII TCCGGA 1 cut(s) 1243
AciI CCGC 5 cut(s) 159, 198, 201, 231, 1512
AclWI GGATC 3 cut(s) 1846, 1859, 2429
AcoI YGGCCR 2 cut(s) 1893, 2339
AcsI RAATTY 8 cut(s) 130, 592, 1095, 1177, 1239, 1843, 1970, 2449
AcuI CTGAAG 4 cut(s) 51, 1379, 2537, 2556
AfaI GTAC 5 cut(s) 427, 836, 877, 2267, 2316
AfiI CCNNNNNNNGG 4 cut(s) 663, 1387, 1902, 2444
AflII CTTAAG 1 cut(s) 904
AflIII ACRYGT 1 cut(s) 1583
AhdI GACNNNNNGTC 1 cut(s) 2399
AjuI GAANNNNNNNTTGG 4 cut(s) 1543, 1575, 1973, 2005
AleI CACNNNNGTG 2 cut(s) 863, 2504
Alw21I GWGCWC 5 cut(s) 327, 383, 523, 1170, 1675
Alw26I GTCTC 2 cut(s) 386, 627
Alw44I GTGCAC 2 cut(s) 379, 519
AlwI GGATC 3 cut(s) 1846, 1859, 2429
Aor13HI TCCGGA 1 cut(s) 1243
AoxI GGCC 6 cut(s) 108, 511, 1681, 1893, 2335, 2339
ApaLI GTGCAC 2 cut(s) 379, 519
ApeKI GCWGC 3 cut(s) 5, 1760, 2201
ApoI RAATTY 8 cut(s) 130, 592, 1095, 1177, 1239, 1843, 1970, 2449
ArsI GACNNNNNNTTYG 2 cut(s) 2135, 2167
Asp700I GAANNNNTTC 1 cut(s) 2452
AspLEI GCGC 3 cut(s) 1393, 2065, 2254
AspS9I GGNCC 2 cut(s) 147, 1376
AsuHPI GGTGA 7 cut(s) 181, 238, 282, 293, 652, 1031, 1175
AsuII TTCGAA 1 cut(s) 1862
AvaII GGWCC 2 cut(s) 147, 1376
BaeGI GKGCMC 2 cut(s) 383, 523
BalI TGGCCA 1 cut(s) 1895
BamHI GGATCC 1 cut(s) 1851
BanII GRGCYC 4 cut(s) 309, 327, 1170, 1675
BarI GAAGNNNNNNTAC 2 cut(s) 562, 594
BauI CACGAG 1 cut(s) 2576
BbsI GAAGAC 2 cut(s) 1851, 2576
Bbv12I GWGCWC 5 cut(s) 327, 383, 523, 1170, 1675
BbvCI CCTCAGC 1 cut(s) 848
BbvI GCAGC 2 cut(s) 1747, 2188
BccI CCATC 4 cut(s) 365, 1732, 2368, 2533
BcoDI GTCTC 2 cut(s) 386, 627
BfaI CTAG 7 cut(s) 605, 746, 1089, 1115, 1268, 2327, 2471
BfmI CTRYAG 4 cut(s) 13, 1599, 1761, 2005
BfoI RGCGCY 1 cut(s) 1394
BfrI CTTAAG 1 cut(s) 904
BfuAI ACCTGC 2 cut(s) 200, 1051
BisI GCNGC 4 cut(s) 6, 201, 1761, 2202
BlsI GCNGC 4 cut(s) 7, 202, 1762, 2203
Bme18I GGWCC 2 cut(s) 147, 1376
BmeRI GACNNNNNGTC 1 cut(s) 2399
BmgT120I GGNCC 2 cut(s) 147, 1376
BmiI GGNNCC 4 cut(s) 308, 735, 1378, 1853
BmrI ACTGGG 1 cut(s) 950
BmsI GCATC 4 cut(s) 510, 732, 828, 2499
BmuI ACTGGG 1 cut(s) 950
BoxI GACNNNNGTC 2 cut(s) 1293, 1586
BpiI GAAGAC 2 cut(s) 1851, 2576
BpmI CTGGAG 2 cut(s) 410, 2342
Bpu10I CCTNAGC 1 cut(s) 848
Bpu14I TTCGAA 1 cut(s) 1862
BpuEI CTTGAG 8 cut(s) 124, 496, 701, 743, 932, 1012, 1659, 2105
Bsa29I ATCGAT 1 cut(s) 1654
BsaJI CCNNGG 4 cut(s) 514, 1321, 1890, 2217
BsaWI WCCGGW 1 cut(s) 1243
Bsc4I CCNNNNNNNGG 4 cut(s) 663, 1387, 1902, 2444
Bse118I RCCGGY 1 cut(s) 2337
Bse1I ACTGG 7 cut(s) 956, 1334, 1378, 1636, 1855, 2076, 2325
Bse3DI GCAATG 3 cut(s) 23, 971, 2058
BseAI TCCGGA 1 cut(s) 1243
BseCI ATCGAT 1 cut(s) 1654
BseDI CCNNGG 4 cut(s) 514, 1321, 1890, 2217
BseGI GGATG 4 cut(s) 357, 501, 1753, 2299
BseLI CCNNNNNNNGG 4 cut(s) 663, 1387, 1902, 2444
BseMI GCAATG 3 cut(s) 23, 971, 2058
BseNI ACTGG 7 cut(s) 956, 1334, 1378, 1636, 1855, 2076, 2325
BseSI GKGCMC 2 cut(s) 383, 523
BseXI GCAGC 2 cut(s) 1747, 2188
BseYI CCCAGC 2 cut(s) 1477, 2457
BsgI GTGCAG 1 cut(s) 178
BshFI GGCC 6 cut(s) 110, 513, 1683, 1895, 2337, 2341
BshVI ATCGAT 1 cut(s) 1654
BsiHKAI GWGCWC 5 cut(s) 327, 383, 523, 1170, 1675
BsiSI CCGG 3 cut(s) 1244, 2338, 2513
BslFI GGGAC 3 cut(s) 1362, 1630, 2542
BslI CCNNNNNNNGG 4 cut(s) 663, 1387, 1902, 2444
BsmAI GTCTC 2 cut(s) 386, 627
BsmFI GGGAC 3 cut(s) 1362, 1630, 2542
BsmI GAATGC 1 cut(s) 905
BsnI GGCC 6 cut(s) 110, 513, 1683, 1895, 2337, 2341
Bsp119I TTCGAA 1 cut(s) 1862
Bsp1286I GDGCHC 6 cut(s) 309, 327, 383, 523, 1170, 1675
Bsp13I TCCGGA 1 cut(s) 1243
Bsp143I GATC 9 cut(s) 616, 1351, 1851, 1881, 1899, 2001, 2434, 2442, 2546
Bsp19I CCATGG 1 cut(s) 2217
BspACI CCGC 5 cut(s) 159, 198, 201, 231, 1512
BspANI GGCC 6 cut(s) 110, 513, 1683, 1895, 2337, 2341
BspDI ATCGAT 1 cut(s) 1654
BspEI TCCGGA 1 cut(s) 1243
BspLI GGNNCC 4 cut(s) 308, 735, 1378, 1853
BspMAI CTGCAG 3 cut(s) 17, 1765, 2009
BspMI ACCTGC 2 cut(s) 200, 1051
BspPI GGATC 3 cut(s) 1846, 1859, 2429
BspT104I TTCGAA 1 cut(s) 1862
BspTI CTTAAG 1 cut(s) 904
BsrDI GCAATG 3 cut(s) 23, 971, 2058
BsrFI RCCGGY 1 cut(s) 2337
BsrI ACTGG 7 cut(s) 956, 1334, 1378, 1636, 1855, 2076, 2325
BssAI RCCGGY 1 cut(s) 2337
BssECI CCNNGG 4 cut(s) 514, 1321, 1890, 2217
BssMI GATC 9 cut(s) 616, 1351, 1851, 1881, 1899, 2001, 2434, 2442, 2546
BssSI CACGAG 1 cut(s) 2576
BssT1I CCWWGG 4 cut(s) 514, 1321, 1890, 2217
Bst2BI CACGAG 1 cut(s) 2576
Bst4CI ACNGT 2 cut(s) 1278, 1295
BstAFI CTTAAG 1 cut(s) 904
BstBI TTCGAA 1 cut(s) 1862
BstC8I GCNNGC 4 cut(s) 1410, 2339, 2343, 2352
BstDSI CCRYGG 1 cut(s) 2217
BstENI CCTNNNNNAGG 1 cut(s) 1385
BstF5I GGATG 4 cut(s) 357, 501, 1753, 2299
BstH2I RGCGCY 1 cut(s) 1394
BstHHI GCGC 3 cut(s) 1393, 2065, 2254
BstKTI GATC 9 cut(s) 619, 1354, 1854, 1884, 1902, 2004, 2437, 2445, 2549
BstMAI GTCTC 2 cut(s) 386, 627
BstMBI GATC 9 cut(s) 616, 1351, 1851, 1881, 1899, 2001, 2434, 2442, 2546
BstMWI GCNNNNNNNGC 6 cut(s) 676, 1390, 1874, 2347, 2351, 2356
BstNSI RCATGY 1 cut(s) 1587
BstPAI GACNNNNGTC 2 cut(s) 1293, 1586
BstSFI CTRYAG 4 cut(s) 13, 1599, 1761, 2005
BstSLI GKGCMC 2 cut(s) 383, 523
BstV1I GCAGC 2 cut(s) 1747, 2188
BstV2I GAAGAC 2 cut(s) 1851, 2576
BstX2I RGATCY 1 cut(s) 1851
BstXI CCANNNNNNTGG 1 cut(s) 182
BstYI RGATCY 1 cut(s) 1851
Bsu15I ATCGAT 1 cut(s) 1654
BsuRI GGCC 6 cut(s) 110, 513, 1683, 1895, 2337, 2341
BsuTUI ATCGAT 1 cut(s) 1654
BtgI CCRYGG 1 cut(s) 2217
BtgZI GCGATG 1 cut(s) 1959
BtsCI GGATG 4 cut(s) 357, 501, 1753, 2299
BtsI GCAGTG 2 cut(s) 169, 2033
BtsIMutI CAGTG 7 cut(s) 169, 383, 1274, 1341, 1629, 1719, 2033
BveI ACCTGC 2 cut(s) 200, 1051
Cac8I GCNNGC 4 cut(s) 1410, 2339, 2343, 2352
CfoI GCGC 3 cut(s) 1393, 2065, 2254
Cfr10I RCCGGY 1 cut(s) 2337
Cfr13I GGNCC 2 cut(s) 147, 1376
ClaI ATCGAT 1 cut(s) 1654
CseI GACGC 1 cut(s) 2153
Csp6I GTAC 5 cut(s) 426, 835, 876, 2266, 2315
CspCI CAANNNNNGTGG 2 cut(s) 643, 678
CviAII CATG 6 cut(s) 49, 488, 1103, 1584, 1727, 2218
CviQI GTAC 5 cut(s) 426, 835, 876, 2266, 2315
DpnI GATC 9 cut(s) 618, 1353, 1853, 1883, 1901, 2003, 2436, 2444, 2548
DpnII GATC 9 cut(s) 616, 1351, 1851, 1881, 1899, 2001, 2434, 2442, 2546
DraI TTTAAA 1 cut(s) 435
DriI GACNNNNNGTC 1 cut(s) 2399
EaeI YGGCCR 2 cut(s) 1893, 2339
Eam1105I GACNNNNNGTC 1 cut(s) 2399
EciI GGCGGA 1 cut(s) 148
Ecl136II GAGCTC 3 cut(s) 325, 1168, 1673
Eco130I CCWWGG 4 cut(s) 514, 1321, 1890, 2217
Eco147I AGGCCT 2 cut(s) 110, 513
Eco24I GRGCYC 4 cut(s) 309, 327, 1170, 1675
Eco32I GATATC 1 cut(s) 20
Eco47I GGWCC 2 cut(s) 147, 1376
Eco53kI GAGCTC 3 cut(s) 325, 1168, 1673
Eco57I CTGAAG 4 cut(s) 51, 1379, 2537, 2556
EcoICRI GAGCTC 3 cut(s) 325, 1168, 1673
EcoNI CCTNNNNNAGG 1 cut(s) 1385
EcoRI GAATTC 1 cut(s) 1970
EcoRV GATATC 1 cut(s) 20
EcoT14I CCWWGG 4 cut(s) 514, 1321, 1890, 2217
EcoT38I GRGCYC 4 cut(s) 309, 327, 1170, 1675
ErhI CCWWGG 4 cut(s) 514, 1321, 1890, 2217
FaeI CATG 6 cut(s) 52, 491, 1106, 1587, 1730, 2221
FalI AAGNNNNNCTT 2 cut(s) 1668, 1700
FaqI GGGAC 3 cut(s) 1362, 1630, 2542
FatI CATG 6 cut(s) 48, 487, 1102, 1583, 1726, 2217
Fnu4HI GCNGC 4 cut(s) 6, 201, 1761, 2202
FokI GGATG 4 cut(s) 344, 488, 1760, 2306
FriOI GRGCYC 4 cut(s) 309, 327, 1170, 1675
FseI GGCCGGCC 1 cut(s) 2341
Fsp4HI GCNGC 4 cut(s) 6, 201, 1761, 2202
FspBI CTAG 7 cut(s) 605, 746, 1089, 1115, 1268, 2327, 2471
FspI TGCGCA 1 cut(s) 2064
GlaI GCGC 3 cut(s) 1392, 2064, 2253
GluI GCNGC 4 cut(s) 6, 201, 1761, 2202
GsaI CCCAGC 2 cut(s) 1481, 2461
GsuI CTGGAG 2 cut(s) 410, 2342
HaeII RGCGCY 1 cut(s) 1394
HaeIII GGCC 6 cut(s) 110, 513, 1683, 1895, 2337, 2341
HapII CCGG 3 cut(s) 1244, 2338, 2513
HgaI GACGC 1 cut(s) 2153
HhaI GCGC 3 cut(s) 1393, 2065, 2254
Hin1II CATG 6 cut(s) 52, 491, 1106, 1587, 1730, 2221
Hin6I GCGC 3 cut(s) 1391, 2063, 2252
HinP1I GCGC 3 cut(s) 1391, 2063, 2252
HincII GTYRAC 2 cut(s) 2214, 2404
HindII GTYRAC 2 cut(s) 2214, 2404
HindIII AAGCTT 7 cut(s) 265, 668, 677, 1367, 1406, 1835, 1866
HpaI GTTAAC 1 cut(s) 2214
HpaII CCGG 3 cut(s) 1244, 2338, 2513
HphI GGTGA 7 cut(s) 181, 238, 282, 293, 652, 1031, 1175
Hpy166II GTNNAC 8 cut(s) 381, 428, 521, 1792, 1805, 2214, 2299, 2404
Hpy8I GTNNAC 8 cut(s) 381, 428, 521, 1792, 1805, 2214, 2299, 2404
Hpy99I CGWCG 1 cut(s) 2397
HpyAV CCTTC 8 cut(s) 68, 303, 488, 588, 863, 1867, 2090, 2147
HpyCH4III ACNGT 2 cut(s) 1278, 1295
HpyF10VI GCNNNNNNNGC 6 cut(s) 676, 1390, 1874, 2347, 2351, 2356
Hsp92II CATG 6 cut(s) 52, 491, 1106, 1587, 1730, 2221
HspAI GCGC 3 cut(s) 1391, 2063, 2252
Kpn2I TCCGGA 1 cut(s) 1243
KroI GCCGGC 1 cut(s) 2337
KroNI GCCGGC 1 cut(s) 2339
KspAI GTTAAC 1 cut(s) 2214
Kzo9I GATC 9 cut(s) 616, 1351, 1851, 1881, 1899, 2001, 2434, 2442, 2546
LmnI GCTCC 7 cut(s) 312, 1057, 1173, 1986, 2323, 2474, 2608
Lsp1109I GCAGC 2 cut(s) 1747, 2188
LweI GCATC 4 cut(s) 510, 732, 828, 2499
MaeI CTAG 7 cut(s) 605, 746, 1089, 1115, 1268, 2327, 2471
MaeIII GTNAC 8 cut(s) 244, 281, 455, 1184, 1272, 1717, 2243, 2329
MalI GATC 9 cut(s) 618, 1353, 1853, 1883, 1901, 2003, 2436, 2444, 2548
MboI GATC 9 cut(s) 616, 1351, 1851, 1881, 1899, 2001, 2434, 2442, 2546
MboII GAAGA 7 cut(s) 86, 337, 1481, 1843, 1851, 2445, 2581
MfeI CAATTG 1 cut(s) 1052
MflI RGATCY 1 cut(s) 1851
MhlI GDGCHC 6 cut(s) 309, 327, 383, 523, 1170, 1675
MlsI TGGCCA 1 cut(s) 1895
MluNI TGGCCA 1 cut(s) 1895
MlyI GAGTC 6 cut(s) 881, 1181, 1467, 1612, 1725, 2558
MmeI TCCRAC 2 cut(s) 174, 1999
Mox20I TGGCCA 1 cut(s) 1895
MroI TCCGGA 1 cut(s) 1243
MroNI GCCGGC 1 cut(s) 2337
MroXI GAANNNNTTC 1 cut(s) 2452
MscI TGGCCA 1 cut(s) 1895
MslI CAYNNNNRTG 3 cut(s) 861, 863, 2504
Msp20I TGGCCA 1 cut(s) 1895
MspCI CTTAAG 1 cut(s) 904
MspI CCGG 3 cut(s) 1244, 2338, 2513
MunI CAATTG 1 cut(s) 1052
Mva1269I GAATGC 1 cut(s) 905
MwoI GCNNNNNNNGC 6 cut(s) 676, 1390, 1874, 2347, 2351, 2356
NaeI GCCGGC 1 cut(s) 2339
NcoI CCATGG 1 cut(s) 2217
NdeII GATC 9 cut(s) 616, 1351, 1851, 1881, 1899, 2001, 2434, 2442, 2546
NgoMIV GCCGGC 1 cut(s) 2337
NlaIII CATG 6 cut(s) 52, 491, 1106, 1587, 1730, 2221
NlaIV GGNNCC 4 cut(s) 308, 735, 1378, 1853
NmuCI GTSAC 6 cut(s) 244, 281, 1184, 1272, 1717, 2329
NsbI TGCGCA 1 cut(s) 2064
NspI RCATGY 1 cut(s) 1587
NspV TTCGAA 1 cut(s) 1862
OliI CACNNNNGTG 2 cut(s) 863, 2504
PaqCI CACCTGC 1 cut(s) 200
PceI AGGCCT 2 cut(s) 110, 513
PciI ACATGT 1 cut(s) 1583
PctI GAATGC 1 cut(s) 905
PdiI GCCGGC 1 cut(s) 2339
PdmI GAANNNNTTC 1 cut(s) 2452
PfeI GAWTC 9 cut(s) 186, 400, 649, 1031, 1134, 1210, 1529, 1949, 2228
PkrI GCNGC 4 cut(s) 7, 202, 1762, 2203
PleI GAGTC 6 cut(s) 881, 1181, 1467, 1612, 1724, 2558
PpsI GAGTC 6 cut(s) 881, 1181, 1467, 1612, 1724, 2558
PscI ACATGT 1 cut(s) 1583
PshAI GACNNNNGTC 2 cut(s) 1293, 1586
PsiI TTATAA 3 cut(s) 842, 1650, 1662
Psp124BI GAGCTC 3 cut(s) 327, 1170, 1675
PspFI CCCAGC 2 cut(s) 1477, 2457
PspN4I GGNNCC 4 cut(s) 308, 735, 1378, 1853
PspPI GGNCC 2 cut(s) 147, 1376
PstI CTGCAG 3 cut(s) 17, 1765, 2009
PsuI RGATCY 1 cut(s) 1851
RigI GGCCGGCC 1 cut(s) 2341
RsaI GTAC 5 cut(s) 427, 836, 877, 2267, 2316
RsaNI GTAC 5 cut(s) 426, 835, 876, 2266, 2315
RseI CAYNNNNRTG 3 cut(s) 861, 863, 2504
SacI GAGCTC 3 cut(s) 327, 1170, 1675
SatI GCNGC 4 cut(s) 6, 201, 1761, 2202
Sau3AI GATC 9 cut(s) 616, 1351, 1851, 1881, 1899, 2001, 2434, 2442, 2546
Sau96I GGNCC 2 cut(s) 147, 1376
SchI GAGTC 6 cut(s) 881, 1181, 1467, 1612, 1725, 2558
SduI GDGCHC 6 cut(s) 309, 327, 383, 523, 1170, 1675
SfaNI GCATC 4 cut(s) 510, 732, 828, 2499
SfcI CTRYAG 4 cut(s) 13, 1599, 1761, 2005
SfuI TTCGAA 1 cut(s) 1862
SinI GGWCC 2 cut(s) 147, 1376
SmiMI CAYNNNNRTG 3 cut(s) 861, 863, 2504
SmlI CTYRAG 9 cut(s) 139, 475, 680, 758, 904, 911, 991, 1674, 2120
SmoI CTYRAG 9 cut(s) 139, 475, 680, 758, 904, 911, 991, 1674, 2120
SseBI AGGCCT 2 cut(s) 110, 513
SsiI CCGC 5 cut(s) 159, 198, 201, 231, 1512
SspMI CTAG 7 cut(s) 605, 746, 1089, 1115, 1268, 2327, 2471
SstI GAGCTC 3 cut(s) 327, 1170, 1675
StuI AGGCCT 2 cut(s) 110, 513
StyI CCWWGG 4 cut(s) 514, 1321, 1890, 2217
TaaI ACNGT 2 cut(s) 1278, 1295
TaqI TCGA 6 cut(s) 1527, 1654, 1862, 1952, 2567, 2589
TatI WGTACW 2 cut(s) 2265, 2314
TauI GCSGC 1 cut(s) 203
TfiI GAWTC 9 cut(s) 186, 400, 649, 1031, 1134, 1210, 1529, 1949, 2228
TscAI CASTG 7 cut(s) 176, 383, 1281, 1341, 1636, 1726, 2033
TseFI GTSAC 6 cut(s) 244, 281, 1184, 1272, 1717, 2329
TseI GCWGC 3 cut(s) 5, 1760, 2201
Tsp45I GTSAC 6 cut(s) 244, 281, 1184, 1272, 1717, 2329
TspDTI ATGAA 5 cut(s) 1223, 1473, 1830, 1983, 2099
TspGWI ACGGA 1 cut(s) 2483
TspRI CASTG 7 cut(s) 176, 383, 1281, 1341, 1636, 1726, 2033
Vha464I CTTAAG 1 cut(s) 904
VneI GTGCAC 2 cut(s) 379, 519
VpaK11BI GGWCC 2 cut(s) 147, 1376
XagI CCTNNNNNAGG 1 cut(s) 1385
XapI RAATTY 8 cut(s) 130, 592, 1095, 1177, 1239, 1843, 1970, 2449
XceI RCATGY 1 cut(s) 1587
XcmI CCANNNNNNNNNTGG 1 cut(s) 963
XmnI GAANNNNTTC 1 cut(s) 2452
XspI CTAG 7 cut(s) 605, 746, 1089, 1115, 1268, 2327, 2471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.