pycom12g05680
NAC Family

Probably inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0006756
Physical Location & Seq
Reverse (-)
5611563 .. 5614988
3426 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g05680.1

Sequence Viewer

Length: 2829 bp
ATGATGTTGCATTTTTTGCTGATAGCCCCCTCGACCCATCAAACAAACCCACCAAATCCTCAACTCCTCTTTCTTCTGACTCTCATAGACTCACTCTCTACCCGCTTCACTGATAGGTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCCCCCTCCTCCTCCTCCTCCAATGGCTGCTTCCTGCAGATATCCATTGCTTCTCTCTCTAACAATCTCCATGTTTTTCCTTCTTACTTCAGCATTGTTATCTGAACCAGACATTCTTCTCACATTCAAGGCCTCCATTAAAGACCCCCAAAATTCTCTCTCAAGCTGGTCCAACACCTCCGCCACCCACCACTGCCAATGGGCTGGAATCACCTGCACCGCCGCCACCACCACCACACTTTCTGTCACCTCCCTCAACCTCCAAAGCTTCAACCTTTCAGGTGACATTTCACCTTCAATCTGTGGGCTCCCTTATCTGTCAGAGCTCAACCTTGCTGACAACTTCTTCAACCAGGCCATCCCTCTCCACCTCTCTCAGTGCACTTCTCTGGAGACTTTGAATCTCAGCAACAATTTAATCTGGGGTACACTTTTAAACCAGATTTCTCAGTTTGGTAACTTGAAAGTTCTTGACTTGAGCATAAACCATGTTGAAGGAAGCATCCCACAAGGCCTTGGTGCACTCCACAACCTCCAAGTTCTCAACTTGGGCAGCAACTTGCTTTCGGGTAATGTGCCTTCTATTTTTGGGAATTTGACTGAGCTAGTTGTTCTTGATCTGTCTCAAAATACATACTTGGTGAGTGAGATTCCCACTGACATTGGGAAGCTTGTGAAGCTTGAGAAGTTGTTCTTGCAAGGCTCTGGTTTTCATGGTGTAATTCCTAATTCCTTGGTGGGTTTACAAAGTTTGAGTGTTTTGGACTTTTCCCAAAATAACTTAACAGGTAGGGTTCCTCAGATGCTAGGGTTTTCTCTCAAGAACTTGGTGTCTTTTGGTGTTTCAGAGAATAGGCTTTCTGGGTTTTTCCCAAATGGCATCTGTAGTGGAAGGTACCTTATAAACCTCAGCCTTCACACAAATGTGTTCAATGGTACAATACCTGACTCCATTAGTGAATGCTTAAGGCTTGAGGTGTTTGAAGTTCAGAACAATTTGTTTTCTGGGGATTTCCCAGTTGGGTTATGGTCATTGCCCAAAATTAAGCTCTTGAGAGCTGAGAATAATCGGTTTTCGGGTGAAATACCTGATTCGGTATCAATGGCTGCTCAATTGGAGCAGGTTCAAATAGATAACAACAGCTTCACTAGCAAAATTCCTCATGGTCTTGGGCTAGTCAAGAGCCTGTATAGATTCTCTGCTTCTCTTAATGGTTTATATGGTGAACTCCCTGCAAATTTCTGTGACTCCCCAGTTATGAGTATAATGAATCTCTCCCACAATTCGCTTTCGGGTCAAATTCCGGAGGTTAAAAAATGCAGGAAGCTAGTGTCATTGTCTTTGGCAGACAACAGTCTTAGTGGGAATATCCCACAATCCCTTGGGGAATTACCAGTGCTAACTTACCTTGATATTTCGGATAATAAGCTTACTGGTCCCCTGCCACAGGCGCTTCAGAACTTGAAGCTTGCACTCTTTAATGTTTCCTTTAATCAGCTATCTGGTAGAGTTCCCTACTCTCTTATTGCAGGACTCCCAGCTTCATTTCTTCAAGGAAATCCTGACCTTTGCGGTCCAGGATTACTCGATTCTTGTTCTGATGACCAGCCAAGACACCATTCTTTTGATTTTACGACCTTGACATGTGCCTTGGTGTCTATAGCTTTTGCTGTTGGAACTCTCATTATCGCTGGTGGGTTAATCTTTTATAATCGATATTATAAACAGAGAGCTCAAGTTGGCCTTTGGCGTTCGGTGTTCTTTTATCCTCTCAGAGTCACTGAGCATGACTTGATGATGGGAATGGATGAGAAAACTGCTGCAGGATGTGGTGGAGTATTTGGTAGAGTTTACATTGTAAGTTTACCAAGTAATGAACTTGTTGCTGTGAAGAAGCTTGTGAATTTTGGGATCGAGTCTTCGAAAGCTTTGAAGGCTGAGATCAAGACCTTGGCCAAGATCAGGCATAAGCACATTGTAAAAGTTCTGGGATTTTGCTATTCCGATGATTCGATATTTCTGATATATGAATTCCAACAGAAAGGGAGCTTGGGGGATTTGATCTGCAGACCTGATTTTAACTTGCAGTGGAGTGTTAGATTGAGAATTGCCATTGGCATTGCGCAAGGACTGGCATACCTTCACAAGGATTATGTTCCTTATTTGCTTCATAGAAATCTCAAATCAAACAATATCCTTTTGGACGCAGAGTTTGAACCAAAGCTCACAGATTTTGCTCTTGACAGAATTGTGGGAGAAGCTGCCTTTCAGTTAACCATGGCTTCGGAATCTGCTCTATCCTGTTACAATGCGCCAGAATGCAAGTACAGTAAGAAAGCAACTGAAAAAATGGATGTTTACAGCTTTGGTGTTGTTCTACTGGAGCTAGTGACAGGCCGGCCAGCAGAGCAAGCAGAGCCATCTAAATCTCTTGACATTGTAAAGTGGGTGCGACGAAAGGTCAACATTACAAATGGGGCTGTCCAAGTTCTTGATCCCAAGATCGGAAATTCTTCCCAGCAAGAGATACTAGGAGCTTTAGAAATCGCTCTCCGTTGCACTTCTGTGATGCCGGAGAAAAGACCATCAATGTCTGAAGTTGTGAGATCACTTCAGTCCCTTGACTCGAAGACCCACGAGGCAGTTATCGACTTCTCTACCTTTGAGGAGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

943

Amino Acids

103.19

Weight (kDa)

6.3

Isoelectric Point (pI)

38.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 85 - 124 2.3e-11 Leucine rich repeat N-terminal domain
LRR_14 PF23598 169 - 281 7.1e-09 Leucine-rich repeat region
LRR_8 PF13855 204 - 261 3e-06 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 663 - 922 4.9e-37 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 663 - 920 2e-35 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1055, 1863, 1875
AarI CACCTGC 1 cut(s) 374
Acc16I TGCGCA 1 cut(s) 2277
Acc36I ACCTGC 2 cut(s) 374, 1264
Acc65I GGTACC 1 cut(s) 1047
AccB1I GGYRCC 1 cut(s) 1047
AccIII TCCGGA 1 cut(s) 1456
AciI CCGC 5 cut(s) 103, 333, 372, 375, 1725
AclWI GGATC 2 cut(s) 2072, 2642
AcoI YGGCCR 2 cut(s) 2106, 2552
AcsI RAATTY 8 cut(s) 304, 745, 1308, 1390, 1452, 2056, 2183, 2662
AcuI CTGAAG 4 cut(s) 225, 1592, 2750, 2769
AfaI GTAC 4 cut(s) 580, 1049, 1090, 2480
AfiI CCNNNNNNNGG 4 cut(s) 1600, 2115, 2299, 2657
AflII CTTAAG 1 cut(s) 1117
AflIII ACRYGT 1 cut(s) 1796
AhdI GACNNNNNGTC 1 cut(s) 2612
AjnI CCWGG 2 cut(s) 504, 1729
AjuI GAANNNNNNNTTGG 6 cut(s) 166, 198, 1756, 1788, 2186, 2218
AleI CACNNNNGTG 2 cut(s) 1076, 2717
Alw21I GWGCWC 4 cut(s) 480, 536, 676, 1888
Alw26I GTCTC 3 cut(s) 123, 539, 780
Alw44I GTGCAC 2 cut(s) 532, 672
AlwI GGATC 2 cut(s) 2072, 2642
Aor13HI TCCGGA 1 cut(s) 1456
AoxI GGCC 7 cut(s) 282, 507, 664, 1894, 2106, 2548, 2552
ApaLI GTGCAC 2 cut(s) 532, 672
ApeKI GCWGC 5 cut(s) 179, 705, 1259, 1973, 2414
ApoI RAATTY 8 cut(s) 304, 745, 1308, 1390, 1452, 2056, 2183, 2662
ArsI GACNNNNNNTTYG 2 cut(s) 2348, 2380
Asp700I GAANNNNTTC 2 cut(s) 842, 2665
Asp718I GGTACC 1 cut(s) 1047
AspLEI GCGC 3 cut(s) 1606, 2278, 2467
AspS9I GGNCC 3 cut(s) 321, 1589, 1727
AsuHPI GGTGA 7 cut(s) 355, 391, 435, 446, 805, 1244, 1388
AsuII TTCGAA 1 cut(s) 2075
AvaII GGWCC 3 cut(s) 321, 1589, 1727
BaeGI GKGCMC 2 cut(s) 536, 676
BalI TGGCCA 1 cut(s) 2108
BanI GGYRCC 1 cut(s) 1047
BanII GRGCYC 3 cut(s) 462, 480, 1888
BarI GAAGNNNNNNTAC 2 cut(s) 715, 747
BauI CACGAG 1 cut(s) 2789
BbsI GAAGAC 2 cut(s) 2064, 2789
Bbv12I GWGCWC 4 cut(s) 480, 536, 676, 1888
BbvCI CCTCAGC 1 cut(s) 1061
BbvI GCAGC 5 cut(s) 166, 717, 1246, 1960, 2401
BccI CCATC 5 cut(s) 45, 518, 1945, 2581, 2746
BciT130I CCWGG 2 cut(s) 506, 1731
BcoDI GTCTC 3 cut(s) 123, 539, 780
BfaI CTAG 7 cut(s) 758, 959, 1302, 1328, 1481, 2540, 2684
BfmI CTRYAG 5 cut(s) 187, 1036, 1812, 1974, 2218
BfoI RGCGCY 1 cut(s) 1607
BfrI CTTAAG 1 cut(s) 1117
BfuAI ACCTGC 2 cut(s) 374, 1264
BisI GCNGC 6 cut(s) 180, 375, 706, 1260, 1974, 2415
BlsI GCNGC 6 cut(s) 181, 376, 707, 1261, 1975, 2416
Bme1390I CCNGG 2 cut(s) 506, 1731
Bme18I GGWCC 3 cut(s) 321, 1589, 1727
BmeRI GACNNNNNGTC 1 cut(s) 2612
BmgT120I GGNCC 3 cut(s) 321, 1589, 1727
BmiI GGNNCC 4 cut(s) 461, 948, 1049, 1591
BmrFI CCNGG 2 cut(s) 506, 1731
BmrI ACTGGG 2 cut(s) 1163, 1400
BmsI GCATC 4 cut(s) 663, 945, 1041, 2712
BmuI ACTGGG 2 cut(s) 1163, 1400
BoxI GACNNNNGTC 1 cut(s) 1506
BpiI GAAGAC 2 cut(s) 2064, 2789
BpmI CTGGAG 2 cut(s) 563, 2555
Bpu10I CCTNAGC 1 cut(s) 1061
Bpu14I TTCGAA 1 cut(s) 2075
BpuEI CTTGAG 7 cut(s) 298, 649, 854, 956, 1145, 1225, 1872
Bsa29I ATCGAT 1 cut(s) 1867
BsaI GGTCTC 1 cut(s) 123
BsaJI CCNNGG 6 cut(s) 667, 885, 1534, 1803, 2103, 2430
BsaWI WCCGGW 1 cut(s) 1456
Bsc4I CCNNNNNNNGG 4 cut(s) 1600, 2115, 2299, 2657
Bse118I RCCGGY 1 cut(s) 2550
Bse1I ACTGG 6 cut(s) 1169, 1406, 1547, 1591, 2289, 2538
Bse3DI GCAATG 3 cut(s) 197, 1184, 2271
BseAI TCCGGA 1 cut(s) 1456
BseBI CCWGG 2 cut(s) 506, 1731
BseCI ATCGAT 1 cut(s) 1867
BseDI CCNNGG 6 cut(s) 667, 885, 1534, 1803, 2103, 2430
BseGI GGATG 5 cut(s) 510, 654, 1966, 1985, 2512
BseLI CCNNNNNNNGG 4 cut(s) 1600, 2115, 2299, 2657
BseMI GCAATG 3 cut(s) 197, 1184, 2271
BseNI ACTGG 6 cut(s) 1169, 1406, 1547, 1591, 2289, 2538
BseRI GAGGAG 5 cut(s) 56, 150, 153, 156, 159
BseSI GKGCMC 2 cut(s) 536, 676
BseXI GCAGC 5 cut(s) 166, 717, 1246, 1960, 2401
BseYI CCCAGC 2 cut(s) 1690, 2670
BsgI GTGCAG 1 cut(s) 352
BshFI GGCC 7 cut(s) 284, 509, 666, 1896, 2108, 2550, 2554
BshNI GGYRCC 1 cut(s) 1047
BshVI ATCGAT 1 cut(s) 1867
BsiHKAI GWGCWC 4 cut(s) 480, 536, 676, 1888
BsiSI CCGG 3 cut(s) 1457, 2551, 2726
BslFI GGGAC 2 cut(s) 1575, 2755
BslI CCNNNNNNNGG 4 cut(s) 1600, 2115, 2299, 2657
BsmAI GTCTC 3 cut(s) 123, 539, 780
BsmFI GGGAC 2 cut(s) 1575, 2755
BsmI GAATGC 2 cut(s) 1118, 2477
BsnI GGCC 7 cut(s) 284, 509, 666, 1896, 2108, 2550, 2554
Bso31I GGTCTC 1 cut(s) 123
Bsp119I TTCGAA 1 cut(s) 2075
Bsp1286I GDGCHC 5 cut(s) 462, 480, 536, 676, 1888
Bsp13I TCCGGA 1 cut(s) 1456
Bsp143I GATC 8 cut(s) 769, 2064, 2094, 2112, 2214, 2647, 2655, 2759
Bsp19I CCATGG 1 cut(s) 2430
BspACI CCGC 5 cut(s) 103, 333, 372, 375, 1725
BspANI GGCC 7 cut(s) 284, 509, 666, 1896, 2108, 2550, 2554
BspDI ATCGAT 1 cut(s) 1867
BspEI TCCGGA 1 cut(s) 1456
BspLI GGNNCC 4 cut(s) 461, 948, 1049, 1591
BspMAI CTGCAG 3 cut(s) 191, 1978, 2222
BspMI ACCTGC 2 cut(s) 374, 1264
BspPI GGATC 2 cut(s) 2072, 2642
BspT104I TTCGAA 1 cut(s) 2075
BspT107I GGYRCC 1 cut(s) 1047
BspTI CTTAAG 1 cut(s) 1117
BspTNI GGTCTC 1 cut(s) 123
BsrDI GCAATG 3 cut(s) 197, 1184, 2271
BsrFI RCCGGY 1 cut(s) 2550
BsrI ACTGG 6 cut(s) 1169, 1406, 1547, 1591, 2289, 2538
BssAI RCCGGY 1 cut(s) 2550
BssECI CCNNGG 6 cut(s) 667, 885, 1534, 1803, 2103, 2430
BssMI GATC 8 cut(s) 769, 2064, 2094, 2112, 2214, 2647, 2655, 2759
BssSI CACGAG 1 cut(s) 2789
BssT1I CCWWGG 6 cut(s) 667, 885, 1534, 1803, 2103, 2430
Bst2BI CACGAG 1 cut(s) 2789
Bst2UI CCWGG 2 cut(s) 506, 1731
Bst4CI ACNGT 2 cut(s) 1508, 2483
BstAFI CTTAAG 1 cut(s) 1117
BstAPI GCANNNNNTGC 1 cut(s) 16
BstBI TTCGAA 1 cut(s) 2075
BstC8I GCNNGC 4 cut(s) 1623, 2552, 2556, 2565
BstDSI CCRYGG 1 cut(s) 2430
BstENI CCTNNNNNAGG 2 cut(s) 1598, 2297
BstF5I GGATG 5 cut(s) 510, 654, 1966, 1985, 2512
BstH2I RGCGCY 1 cut(s) 1607
BstHHI GCGC 3 cut(s) 1606, 2278, 2467
BstKTI GATC 8 cut(s) 772, 2067, 2097, 2115, 2217, 2650, 2658, 2762
BstMAI GTCTC 3 cut(s) 123, 539, 780
BstMBI GATC 8 cut(s) 769, 2064, 2094, 2112, 2214, 2647, 2655, 2759
BstMWI GCNNNNNNNGC 8 cut(s) 16, 829, 1302, 1603, 2087, 2560, 2564, 2569
BstNI CCWGG 2 cut(s) 506, 1731
BstNSI RCATGY 1 cut(s) 1800
BstPAI GACNNNNGTC 1 cut(s) 1506
BstSCI CCNGG 2 cut(s) 504, 1729
BstSFI CTRYAG 5 cut(s) 187, 1036, 1812, 1974, 2218
BstSLI GKGCMC 2 cut(s) 536, 676
BstV1I GCAGC 5 cut(s) 166, 717, 1246, 1960, 2401
BstV2I GAAGAC 2 cut(s) 2064, 2789
BstXI CCANNNNNNTGG 1 cut(s) 356
Bsu15I ATCGAT 1 cut(s) 1867
BsuRI GGCC 7 cut(s) 284, 509, 666, 1896, 2108, 2550, 2554
BsuTUI ATCGAT 1 cut(s) 1867
BtgI CCRYGG 1 cut(s) 2430
BtsCI GGATG 5 cut(s) 510, 654, 1966, 1985, 2512
BtsI GCAGTG 2 cut(s) 343, 2246
BtsIMutI CAGTG 7 cut(s) 108, 343, 536, 807, 1554, 1932, 2246
BveI ACCTGC 2 cut(s) 374, 1264
Cac8I GCNNGC 4 cut(s) 1623, 2552, 2556, 2565
CfoI GCGC 3 cut(s) 1606, 2278, 2467
Cfr10I RCCGGY 1 cut(s) 2550
Cfr13I GGNCC 3 cut(s) 321, 1589, 1727
ClaI ATCGAT 1 cut(s) 1867
CseI GACGC 1 cut(s) 2366
Csp6I GTAC 4 cut(s) 579, 1048, 1089, 2479
CspCI CAANNNNNGTGG 2 cut(s) 796, 831
CviAII CATG 7 cut(s) 223, 641, 866, 1316, 1797, 1940, 2431
CviQI GTAC 4 cut(s) 579, 1048, 1089, 2479
DpnI GATC 8 cut(s) 771, 2066, 2096, 2114, 2216, 2649, 2657, 2761
DpnII GATC 8 cut(s) 769, 2064, 2094, 2112, 2214, 2647, 2655, 2759
DraI TTTAAA 1 cut(s) 588
DriI GACNNNNNGTC 1 cut(s) 2612
EaeI YGGCCR 2 cut(s) 2106, 2552
Eam1105I GACNNNNNGTC 1 cut(s) 2612
EciI GGCGGA 1 cut(s) 322
Ecl136II GAGCTC 2 cut(s) 478, 1886
Eco130I CCWWGG 6 cut(s) 667, 885, 1534, 1803, 2103, 2430
Eco147I AGGCCT 2 cut(s) 284, 666
Eco24I GRGCYC 3 cut(s) 462, 480, 1888
Eco31I GGTCTC 1 cut(s) 123
Eco32I GATATC 1 cut(s) 194
Eco47I GGWCC 3 cut(s) 321, 1589, 1727
Eco53kI GAGCTC 2 cut(s) 478, 1886
Eco57I CTGAAG 4 cut(s) 225, 1592, 2750, 2769
EcoICRI GAGCTC 2 cut(s) 478, 1886
EcoNI CCTNNNNNAGG 2 cut(s) 1598, 2297
EcoRI GAATTC 1 cut(s) 2183
EcoRII CCWGG 2 cut(s) 504, 1729
EcoRV GATATC 1 cut(s) 194
EcoT14I CCWWGG 6 cut(s) 667, 885, 1534, 1803, 2103, 2430
EcoT38I GRGCYC 3 cut(s) 462, 480, 1888
ErhI CCWWGG 6 cut(s) 667, 885, 1534, 1803, 2103, 2430
FaeI CATG 7 cut(s) 226, 644, 869, 1319, 1800, 1943, 2434
FalI AAGNNNNNCTT 4 cut(s) 830, 862, 1881, 1913
FaqI GGGAC 2 cut(s) 1575, 2755
FatI CATG 7 cut(s) 222, 640, 865, 1315, 1796, 1939, 2430
FauI CCCGC 1 cut(s) 110
Fnu4HI GCNGC 6 cut(s) 180, 375, 706, 1260, 1974, 2415
FokI GGATG 5 cut(s) 497, 641, 1973, 1992, 2519
FriOI GRGCYC 3 cut(s) 462, 480, 1888
FseI GGCCGGCC 1 cut(s) 2554
Fsp4HI GCNGC 6 cut(s) 180, 375, 706, 1260, 1974, 2415
FspBI CTAG 7 cut(s) 758, 959, 1302, 1328, 1481, 2540, 2684
FspI TGCGCA 1 cut(s) 2277
GlaI GCGC 3 cut(s) 1605, 2277, 2466
GluI GCNGC 6 cut(s) 180, 375, 706, 1260, 1974, 2415
GsaI CCCAGC 2 cut(s) 1694, 2674
GsuI CTGGAG 2 cut(s) 563, 2555
HaeII RGCGCY 1 cut(s) 1607
HaeIII GGCC 7 cut(s) 284, 509, 666, 1896, 2108, 2550, 2554
HapII CCGG 3 cut(s) 1457, 2551, 2726
HgaI GACGC 1 cut(s) 2366
HhaI GCGC 3 cut(s) 1606, 2278, 2467
Hin1II CATG 7 cut(s) 226, 644, 869, 1319, 1800, 1943, 2434
Hin6I GCGC 3 cut(s) 1604, 2276, 2465
HinP1I GCGC 3 cut(s) 1604, 2276, 2465
HincII GTYRAC 2 cut(s) 2427, 2617
HindII GTYRAC 2 cut(s) 2427, 2617
HindIII AAGCTT 7 cut(s) 418, 821, 830, 1580, 1619, 2048, 2079
HpaI GTTAAC 1 cut(s) 2427
HpaII CCGG 3 cut(s) 1457, 2551, 2726
HphI GGTGA 7 cut(s) 355, 391, 435, 446, 805, 1244, 1388
Hpy99I CGWCG 1 cut(s) 2610
HpyAV CCTTC 8 cut(s) 242, 456, 641, 741, 1038, 1076, 2080, 2303
HpyCH4III ACNGT 2 cut(s) 1508, 2483
HpyF10VI GCNNNNNNNGC 8 cut(s) 16, 829, 1302, 1603, 2087, 2560, 2564, 2569
Hsp92II CATG 7 cut(s) 226, 644, 869, 1319, 1800, 1943, 2434
HspAI GCGC 3 cut(s) 1604, 2276, 2465
Kpn2I TCCGGA 1 cut(s) 1456
KpnI GGTACC 1 cut(s) 1051
KroI GCCGGC 1 cut(s) 2550
KroNI GCCGGC 1 cut(s) 2552
KspAI GTTAAC 1 cut(s) 2427
Kzo9I GATC 8 cut(s) 769, 2064, 2094, 2112, 2214, 2647, 2655, 2759
LmnI GCTCC 6 cut(s) 465, 1270, 2199, 2536, 2687, 2821
Lsp1109I GCAGC 5 cut(s) 166, 717, 1246, 1960, 2401
LweI GCATC 4 cut(s) 663, 945, 1041, 2712
MaeI CTAG 7 cut(s) 758, 959, 1302, 1328, 1481, 2540, 2684
MaeIII GTNAC 7 cut(s) 397, 434, 608, 1397, 1930, 2456, 2542
MalI GATC 8 cut(s) 771, 2066, 2096, 2114, 2216, 2649, 2657, 2761
MboI GATC 8 cut(s) 769, 2064, 2094, 2112, 2214, 2647, 2655, 2759
MboII GAAGA 8 cut(s) 65, 260, 490, 1694, 2056, 2064, 2658, 2794
MfeI CAATTG 1 cut(s) 1265
MhlI GDGCHC 5 cut(s) 462, 480, 536, 676, 1888
MlsI TGGCCA 1 cut(s) 2108
MluNI TGGCCA 1 cut(s) 2108
MlyI GAGTC 8 cut(s) 73, 83, 1094, 1394, 1680, 1938, 2078, 2771
MmeI TCCRAC 3 cut(s) 348, 1807, 2212
Mox20I TGGCCA 1 cut(s) 2108
MroI TCCGGA 1 cut(s) 1456
MroNI GCCGGC 1 cut(s) 2550
MroXI GAANNNNTTC 2 cut(s) 842, 2665
MscI TGGCCA 1 cut(s) 2108
MslI CAYNNNNRTG 3 cut(s) 1074, 1076, 2717
Msp20I TGGCCA 1 cut(s) 2108
MspCI CTTAAG 1 cut(s) 1117
MspI CCGG 3 cut(s) 1457, 2551, 2726
MspR9I CCNGG 2 cut(s) 506, 1731
MunI CAATTG 1 cut(s) 1265
Mva1269I GAATGC 2 cut(s) 1118, 2477
MvaI CCWGG 2 cut(s) 506, 1731
MwoI GCNNNNNNNGC 8 cut(s) 16, 829, 1302, 1603, 2087, 2560, 2564, 2569
NaeI GCCGGC 1 cut(s) 2552
NcoI CCATGG 1 cut(s) 2430
NdeII GATC 8 cut(s) 769, 2064, 2094, 2112, 2214, 2647, 2655, 2759
NgoMIV GCCGGC 1 cut(s) 2550
NlaIII CATG 7 cut(s) 226, 644, 869, 1319, 1800, 1943, 2434
NlaIV GGNNCC 4 cut(s) 461, 948, 1049, 1591
NmuCI GTSAC 5 cut(s) 397, 434, 1397, 1930, 2542
NsbI TGCGCA 1 cut(s) 2277
NspI RCATGY 1 cut(s) 1800
NspV TTCGAA 1 cut(s) 2075
OliI CACNNNNGTG 2 cut(s) 1076, 2717
PaqCI CACCTGC 1 cut(s) 374
PceI AGGCCT 2 cut(s) 284, 666
PciI ACATGT 1 cut(s) 1796
PctI GAATGC 2 cut(s) 1118, 2477
PdiI GCCGGC 1 cut(s) 2552
PdmI GAANNNNTTC 2 cut(s) 842, 2665
PfeI GAWTC 9 cut(s) 360, 553, 802, 1244, 1347, 1423, 1742, 2162, 2441
PfoI TCCNGGA 1 cut(s) 1729
PkrI GCNGC 6 cut(s) 181, 376, 707, 1261, 1975, 2416
PleI GAGTC 8 cut(s) 73, 83, 1094, 1394, 1680, 1937, 2077, 2771
PpsI GAGTC 8 cut(s) 73, 83, 1094, 1394, 1680, 1937, 2077, 2771
PscI ACATGT 1 cut(s) 1796
PshAI GACNNNNGTC 1 cut(s) 1506
PsiI TTATAA 3 cut(s) 1055, 1863, 1875
Psp124BI GAGCTC 2 cut(s) 480, 1888
Psp6I CCWGG 2 cut(s) 504, 1729
PspFI CCCAGC 2 cut(s) 1690, 2670
PspGI CCWGG 2 cut(s) 504, 1729
PspN4I GGNNCC 4 cut(s) 461, 948, 1049, 1591
PspPI GGNCC 3 cut(s) 321, 1589, 1727
PstI CTGCAG 3 cut(s) 191, 1978, 2222
RigI GGCCGGCC 1 cut(s) 2554
RsaI GTAC 4 cut(s) 580, 1049, 1090, 2480
RsaNI GTAC 4 cut(s) 579, 1048, 1089, 2479
RseI CAYNNNNRTG 3 cut(s) 1074, 1076, 2717
SacI GAGCTC 2 cut(s) 480, 1888
SatI GCNGC 6 cut(s) 180, 375, 706, 1260, 1974, 2415
Sau3AI GATC 8 cut(s) 769, 2064, 2094, 2112, 2214, 2647, 2655, 2759
Sau96I GGNCC 3 cut(s) 321, 1589, 1727
SchI GAGTC 8 cut(s) 73, 83, 1094, 1394, 1680, 1938, 2078, 2771
ScrFI CCNGG 2 cut(s) 506, 1731
SduI GDGCHC 5 cut(s) 462, 480, 536, 676, 1888
SfaNI GCATC 4 cut(s) 663, 945, 1041, 2712
SfcI CTRYAG 5 cut(s) 187, 1036, 1812, 1974, 2218
SfuI TTCGAA 1 cut(s) 2075
SinI GGWCC 3 cut(s) 321, 1589, 1727
SmiMI CAYNNNNRTG 3 cut(s) 1074, 1076, 2717
SmlI CTYRAG 8 cut(s) 313, 628, 833, 971, 1117, 1124, 1204, 1887
SmoI CTYRAG 8 cut(s) 313, 628, 833, 971, 1117, 1124, 1204, 1887
SseBI AGGCCT 2 cut(s) 284, 666
SsiI CCGC 5 cut(s) 103, 333, 372, 375, 1725
SspMI CTAG 7 cut(s) 758, 959, 1302, 1328, 1481, 2540, 2684
SstI GAGCTC 2 cut(s) 480, 1888
StuI AGGCCT 2 cut(s) 284, 666
StyD4I CCNGG 2 cut(s) 504, 1729
StyI CCWWGG 6 cut(s) 667, 885, 1534, 1803, 2103, 2430
TaaI ACNGT 2 cut(s) 1508, 2483
TaqI TCGA 8 cut(s) 32, 1740, 1867, 2067, 2075, 2165, 2780, 2802
TatI WGTACW 1 cut(s) 2478
TauI GCSGC 1 cut(s) 377
TfiI GAWTC 9 cut(s) 360, 553, 802, 1244, 1347, 1423, 1742, 2162, 2441
TscAI CASTG 7 cut(s) 115, 350, 536, 814, 1554, 1939, 2246
TseFI GTSAC 5 cut(s) 397, 434, 1397, 1930, 2542
TseI GCWGC 5 cut(s) 179, 705, 1259, 1973, 2414
Tsp45I GTSAC 5 cut(s) 397, 434, 1397, 1930, 2542
TspDTI ATGAA 6 cut(s) 854, 1436, 1686, 2043, 2196, 2312
TspGWI ACGGA 1 cut(s) 2696
TspRI CASTG 7 cut(s) 115, 350, 536, 814, 1554, 1939, 2246
Vha464I CTTAAG 1 cut(s) 1117
VneI GTGCAC 2 cut(s) 532, 672
VpaK11BI GGWCC 3 cut(s) 321, 1589, 1727
XagI CCTNNNNNAGG 2 cut(s) 1598, 2297
XapI RAATTY 8 cut(s) 304, 745, 1308, 1390, 1452, 2056, 2183, 2662
XceI RCATGY 1 cut(s) 1800
XcmI CCANNNNNNNNNTGG 1 cut(s) 1176
XmnI GAANNNNTTC 2 cut(s) 842, 2665
XspI CTAG 7 cut(s) 758, 959, 1302, 1328, 1481, 2540, 2684
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.