MD14G1065000.v1.1
NAC Family

Probably inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0006756
Physical Location & Seq
Reverse (-)
6892678 .. 6896400
3723 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1065000.v1.1.491

Sequence Viewer

Length: 2652 bp
ATGGCTACTTCCTGCAAACATCCTTTACTTCTCTCTCTAATCTCCACATTTTTCCTTCTCACTTCAGCATTATTATCAGAACCAGACATTCTTCTCACCTTCAAGGCCTCCATTAAAGACCCCCAAAACTCACTCTCAACCTGGTCCAACACCTCGGGCACCCACCACTGCCAATGGGCTGGAATCAACTGCACTGCCACCACCACCACCACACTCTCCGTCACCTCCCTCAACCTCCAAAGCTTCAATCTTTCAGGTGACATCTCACCCGCAATCTGTGGGCTCCCTTATCTGTCAGAGCTCAACCTTGCAGGCAACTTCTTCAACCAGCCCATCCCTCTCCACCTCTCTCAGTGCACTTCTCTGGAGACTTTGAATCTCAGCAACAATTTAATCTGGGGTACAATCTTGAACCAGATTTCTCAGTTTGGTAACTTGAAAGTTCTCGACTTGAGCAAAAACCGTGTTGAAGGAAACATCCCGCAAGCCATTGGTGCACTCCACAGCCTCCAATTTCTCAACTTGGGAAGCAACTTGCTTACGGGTAGTGTGCCCTCTATTTTTGCGAATTTGACTGAGCTAGTTGTTCTTGATCTGTCCCAAAATACGTACTTGGTGAGTGAGATTCCTACAGACATTGGGAAGCTTGTGAAGCTTGAGAAGTTGTTCTTGCAAAGCTCTGGTTTTCATGGTGTAATTCCTAATTCTTTGGTGGGTCTACAAAGTTTGAGTGTTTTGGACTTTTCCCAAAATAACTTAACAGGTAGGGTTCCTCAGATACTAGGGTTTTCTCTCAAAAACTTGGTGTCCTTTGATGTTTCAGAGAATAGGCTTTCTGGGTTTTTCCCAAATGGCATCTGTAACGGAAAATACCTTATAAACCTCAGCCTTCACACAAATGTGTTCAATGGTACAATACCGAACTCCATTAGTGAATGCTCAAGGCTTGAGGTGTTTGAAGTTCAAAACAATTTGTTTTCTGGGGATTTCCCAGTTGGGCTATGGTCATTGCCCAAAATTAAGCTCTTAAGAGCTGAGAATAACCGGTTTTCGGGAGAAATACCTGATTCAGTATCAATGGCTGGTCAGTTGGAGCAGGTTCAAATAGATAACAACAGCTTCACTGGCAAAATTCCTCATGGTCTTGGGTTAGTCAAGAGTTTATATAGATTCTCGGCTTCTCTTAATGGTTTATATGGTGAACTCCCAACAAACTTCTGTGACTCCCCTGTTATGAGTATAGTGAATCTCTCCCACAATGCGCTCTTGGGTCAAATTCCGGAGGTTAAGAATTGCAGGAAGCTAGTGTCATTGTCTTTGGCAGACAACAATCTTAGTGGGAGTATCCCAGAATCTCTTGGTGAATTACCAGTGCTAACTTACCTTGATCTTTCTGACAATAAGCTAACCGGTCCCCTTCCACAGGCGCTTCAGAACTTGAAGCTTGCACTCTTCAATGTTTCCTTTAATCAGCTATCTGGTAGAGTCCCCTACTCTCTTATCGCAGGCCTCCCAGCTTCATTTCTTCAAGGAAATCCTGACCTTTGCGGTCCAGGATTACTCGACTCTTGTTCTGATGACCAGCCAAGACACCATTCTTCTGATCTTACGACATTGACAAGTGCCTTGATATCTTTAGCTTTTGCTGTTGGAGCTCTGATTATTGCTGGTGGATTTATTTTTTATCATCGATATTATAAGCAGCGAGCTCAAGTTGGCCTTTGGCGTTCGGTGTTCTTTTATCCTCTCAGAGTCACTGAGCATGACTTGATGATGGGAATGGATGAGAAATCTGCTGCAGGGTGTGGTGGGGTGTTTGGTAGAGTTTACATTGTAAGTTTACCAAGTGGTGAACTTGTTGCTGTGAAGAAGCTTGTGAATTTCGGGATCCAGTCTTCGAAAACTTTGAAGGCTGAGATCAAGACCTTGGCCAAGATAAGGCATAAGAACATTGTAAAAGTTCTGGGATTCTGCTATTCCGATGATTCAATATTTCTGATATATGAGTTCCAACAGAAAGGGAGCTTGGGGGACTTGATCTGCAGACCTGATTTTAATTTGCAGTGGAGTGTTAGATTGAGAATTGCCATTGGCATTGCGCAAGGACTGGCATACCTCCACAAGGATTATGTTCCTCATTTGCTTCATAGAAATCTCAAGTCAAACAATATCCTTCTGGATGCAGAGTTTGAACCAAAGCTCACAGATTTTGCTCTCGACAGAATTGTGGGAGAAGCTGCCTTTCAATCAACCTTGGCTTCAGAATCTACTTTATCCTGTTACAATGCGCCAGAATGCAAGTACACTAAGAAAGTAACTGAACAAATGGATGTTTACAGCTACGGTGTTGTACTATTGGAGCTCGTGACAGGCCGGCCAGCTGAGCAAGCGGAGCCATCCGAGTCTCTTGACATTGTAAAGTGGGTGCGACGGAAGGTCAACATTACAAATGGCGCTGTCCAAGTTCTCGATCCCAAAATAAAAAATTCTTCCCAGCAAGAGATACTAGGAGCTTTAGAAATCGCTCTCTGTTGCACTTCTGTGATGCCGGAGAAAAGACCTTCCATGTCTGAAGTCGTGAGATCACTTCAGTCCCTGGACTCGAGGACCCACGGGGCAGTTATCGACTTCTCTTCCTTTGAGGAACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

884

Amino Acids

96.6

Weight (kDa)

6.27

Isoelectric Point (pI)

36.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 26 - 65 1.2e-09 Leucine rich repeat N-terminal domain
LRR_14 PF23598 115 - 223 4.4e-10 Leucine-rich repeat region
LRR_8 PF13855 121 - 180 1.1e-06 Leucine rich repeat
LRR_8 PF13855 145 - 202 1.9e-07 Leucine rich repeat
LRR_8 PF13855 433 - 492 9.2e-07 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 604 - 863 3.8e-37 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 604 - 861 4.3e-36 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 878, 1698
Acc16I TGCGCA 1 cut(s) 2100
Acc36I ACCTGC 1 cut(s) 1087
AccB1I GGYRCC 1 cut(s) 158
AccI GTMKAC 1 cut(s) 718
AccIII TCCGGA 1 cut(s) 1279
AciI CCGC 4 cut(s) 270, 482, 1548, 2390
AclWI GGATC 3 cut(s) 1882, 1895, 2465
AcoI YGGCCR 2 cut(s) 1929, 2375
AcsI RAATTY 5 cut(s) 568, 1131, 1275, 1879, 2485
AcuI CTGAAG 5 cut(s) 48, 1415, 2244, 2573, 2592
AfaI GTAC 5 cut(s) 403, 611, 913, 2303, 2352
AfiI CCNNNNNNNGG 4 cut(s) 1051, 1423, 1938, 2122
AflII CTTAAG 1 cut(s) 1027
AgeI ACCGGT 2 cut(s) 1044, 1409
AhdI GACNNNNNGTC 1 cut(s) 2435
AjnI CCWGG 3 cut(s) 140, 1552, 2595
AjuI GAANNNNNNNTTGG 4 cut(s) 1579, 1611, 2009, 2041
AleI CACNNNNGTG 2 cut(s) 899, 2540
Alw21I GWGCWC 6 cut(s) 303, 359, 499, 1657, 1711, 2364
Alw26I GTCTC 2 cut(s) 362, 2409
Alw44I GTGCAC 2 cut(s) 355, 495
AlwI GGATC 3 cut(s) 1882, 1895, 2465
AlwNI CAGNNNCTG 1 cut(s) 2596
Ama87I CYCGRG 2 cut(s) 154, 2602
Aor13HI TCCGGA 1 cut(s) 1279
AoxI GGCC 6 cut(s) 105, 1507, 1717, 1929, 2371, 2375
ApaLI GTGCAC 2 cut(s) 355, 495
ApeKI GCWGC 3 cut(s) 1702, 1796, 2237
ApoI RAATTY 5 cut(s) 568, 1131, 1275, 1879, 2485
AsiGI ACCGGT 2 cut(s) 1044, 1409
Asp700I GAANNNNTTC 1 cut(s) 665
AspLEI GCGC 5 cut(s) 1264, 1429, 2101, 2290, 2456
AspS9I GGNCC 4 cut(s) 144, 1412, 1550, 2607
AsuHPI GGTGA 8 cut(s) 88, 214, 258, 269, 628, 1211, 1373, 1862
AsuII TTCGAA 1 cut(s) 1898
AvaI CYCGRG 2 cut(s) 154, 2602
AvaII GGWCC 4 cut(s) 144, 1412, 1550, 2607
BaeGI GKGCMC 4 cut(s) 161, 359, 499, 555
BaeI ACNNNNGTAYC 2 cut(s) 770, 803
BalI TGGCCA 1 cut(s) 1931
BamHI GGATCC 1 cut(s) 1887
BanI GGYRCC 1 cut(s) 158
BanII GRGCYC 5 cut(s) 285, 303, 1657, 1711, 2364
BauI CACGAG 1 cut(s) 2363
BbsI GAAGAC 1 cut(s) 1887
Bbv12I GWGCWC 6 cut(s) 303, 359, 499, 1657, 1711, 2364
BbvCI CCTCAGC 1 cut(s) 884
BbvI GCAGC 3 cut(s) 1714, 1783, 2224
BccI CCATC 3 cut(s) 341, 1768, 2404
BciT130I CCWGG 3 cut(s) 142, 1554, 2597
BciVI GTATCC 1 cut(s) 1355
BcoDI GTCTC 2 cut(s) 362, 2409
BfaI CTAG 4 cut(s) 581, 782, 1304, 2507
BfmI CTRYAG 3 cut(s) 630, 1797, 2041
BfoI RGCGCY 2 cut(s) 1430, 2457
BfrI CTTAAG 1 cut(s) 1027
BfuAI ACCTGC 1 cut(s) 1087
BfuI GTATCC 1 cut(s) 1355
BisI GCNGC 3 cut(s) 1703, 1797, 2238
BlpI GCTNAGC 1 cut(s) 2382
BlsI GCNGC 3 cut(s) 1704, 1798, 2239
Bme1390I CCNGG 3 cut(s) 142, 1554, 2597
Bme18I GGWCC 4 cut(s) 144, 1412, 1550, 2607
BmeRI GACNNNNNGTC 1 cut(s) 2435
BmeT110I CYCGRG 2 cut(s) 154, 2602
BmgT120I GGNCC 4 cut(s) 144, 1412, 1550, 2607
BmiI GGNNCC 7 cut(s) 160, 284, 771, 1414, 1889, 2394, 2609
BmrFI CCNGG 3 cut(s) 142, 1554, 2597
BmrI ACTGGG 1 cut(s) 986
BmsI GCATC 3 cut(s) 864, 2170, 2535
BmuI ACTGGG 1 cut(s) 986
BpiI GAAGAC 1 cut(s) 1887
BpmI CTGGAG 1 cut(s) 386
Bpu10I CCTNAGC 1 cut(s) 884
Bpu1102I GCTNAGC 1 cut(s) 2382
Bpu14I TTCGAA 1 cut(s) 1898
BpuEI CTTGAG 6 cut(s) 472, 677, 925, 968, 1695, 2141
Bsa29I ATCGAT 1 cut(s) 1690
BsaAI YACGTR 1 cut(s) 609
BsaJI CCNNGG 5 cut(s) 153, 1926, 2253, 2595, 2611
BsaWI WCCGGW 3 cut(s) 1044, 1279, 1409
Bsc4I CCNNNNNNNGG 4 cut(s) 1051, 1423, 1938, 2122
Bse118I RCCGGY 3 cut(s) 1044, 1409, 2373
Bse1I ACTGG 5 cut(s) 992, 1129, 1370, 1891, 2112
Bse3DI GCAATG 2 cut(s) 1007, 2094
BseAI TCCGGA 1 cut(s) 1279
BseBI CCWGG 3 cut(s) 142, 1554, 2597
BseCI ATCGAT 1 cut(s) 1690
BseDI CCNNGG 5 cut(s) 153, 1926, 2253, 2595, 2611
BseGI GGATG 7 cut(s) 19, 333, 477, 1789, 2185, 2335, 2396
BseLI CCNNNNNNNGG 4 cut(s) 1051, 1423, 1938, 2122
BseMI GCAATG 2 cut(s) 1007, 2094
BseNI ACTGG 5 cut(s) 992, 1129, 1370, 1891, 2112
BseSI GKGCMC 4 cut(s) 161, 359, 499, 555
BseXI GCAGC 3 cut(s) 1714, 1783, 2224
BseYI CCCAGC 2 cut(s) 1513, 2493
BsgI GTGCAG 1 cut(s) 175
BshFI GGCC 6 cut(s) 107, 1509, 1719, 1931, 2373, 2377
BshNI GGYRCC 1 cut(s) 158
BshTI ACCGGT 2 cut(s) 1044, 1409
BshVI ATCGAT 1 cut(s) 1690
BsiHKAI GWGCWC 6 cut(s) 303, 359, 499, 1657, 1711, 2364
BsiHKCI CYCGRG 2 cut(s) 154, 2602
BsiSI CCGG 5 cut(s) 1045, 1280, 1410, 2374, 2549
BslFI GGGAC 5 cut(s) 583, 1398, 1472, 2045, 2578
BslI CCNNNNNNNGG 4 cut(s) 1051, 1423, 1938, 2122
BsmAI GTCTC 2 cut(s) 362, 2409
BsmFI GGGAC 5 cut(s) 583, 1398, 1472, 2045, 2578
BsmI GAATGC 2 cut(s) 941, 2300
BsnI GGCC 6 cut(s) 107, 1509, 1719, 1931, 2373, 2377
BsoBI CYCGRG 2 cut(s) 154, 2602
Bsp119I TTCGAA 1 cut(s) 1898
Bsp1286I GDGCHC 9 cut(s) 161, 285, 303, 359, 499, 555, 1657, 1711, 2364
Bsp13I TCCGGA 1 cut(s) 1279
Bsp143I GATC 8 cut(s) 592, 1387, 1603, 1887, 1917, 2037, 2470, 2582
Bsp1720I GCTNAGC 1 cut(s) 2382
BspACI CCGC 4 cut(s) 270, 482, 1548, 2390
BspANI GGCC 6 cut(s) 107, 1509, 1719, 1931, 2373, 2377
BspDI ATCGAT 1 cut(s) 1690
BspEI TCCGGA 1 cut(s) 1279
BspLI GGNNCC 7 cut(s) 160, 284, 771, 1414, 1889, 2394, 2609
BspMAI CTGCAG 2 cut(s) 1801, 2045
BspMI ACCTGC 1 cut(s) 1087
BspPI GGATC 3 cut(s) 1882, 1895, 2465
BspT104I TTCGAA 1 cut(s) 1898
BspT107I GGYRCC 1 cut(s) 158
BspTI CTTAAG 1 cut(s) 1027
BsrDI GCAATG 2 cut(s) 1007, 2094
BsrFI RCCGGY 3 cut(s) 1044, 1409, 2373
BsrI ACTGG 5 cut(s) 992, 1129, 1370, 1891, 2112
BssAI RCCGGY 3 cut(s) 1044, 1409, 2373
BssECI CCNNGG 5 cut(s) 153, 1926, 2253, 2595, 2611
BssMI GATC 8 cut(s) 592, 1387, 1603, 1887, 1917, 2037, 2470, 2582
BssSI CACGAG 1 cut(s) 2363
BssT1I CCWWGG 2 cut(s) 1926, 2253
Bst2BI CACGAG 1 cut(s) 2363
Bst2UI CCWGG 3 cut(s) 142, 1554, 2597
Bst4CI ACNGT 2 cut(s) 464, 2345
Bst6I CTCTTC 2 cut(s) 1457, 2638
BstAFI CTTAAG 1 cut(s) 1027
BstBAI YACGTR 1 cut(s) 609
BstBI TTCGAA 1 cut(s) 1898
BstC8I GCNNGC 8 cut(s) 313, 486, 1446, 1507, 1707, 2375, 2379, 2388
BstDSI CCRYGG 1 cut(s) 2611
BstENI CCTNNNNNAGG 2 cut(s) 1421, 2120
BstF5I GGATG 7 cut(s) 19, 333, 477, 1789, 2185, 2335, 2396
BstH2I RGCGCY 2 cut(s) 1430, 2457
BstHHI GCGC 5 cut(s) 1264, 1429, 2101, 2290, 2456
BstKTI GATC 8 cut(s) 595, 1390, 1606, 1890, 1920, 2040, 2473, 2585
BstMAI GTCTC 2 cut(s) 362, 2409
BstMBI GATC 8 cut(s) 592, 1387, 1603, 1887, 1917, 2037, 2470, 2582
BstMWI GCNNNNNNNGC 7 cut(s) 494, 652, 1125, 1652, 2383, 2387, 2392
BstNI CCWGG 3 cut(s) 142, 1554, 2597
BstSCI CCNGG 3 cut(s) 140, 1552, 2595
BstSFI CTRYAG 3 cut(s) 630, 1797, 2041
BstSLI GKGCMC 4 cut(s) 161, 359, 499, 555
BstSNI TACGTA 1 cut(s) 609
BstV1I GCAGC 3 cut(s) 1714, 1783, 2224
BstV2I GAAGAC 1 cut(s) 1887
BstX2I RGATCY 1 cut(s) 1887
BstXI CCANNNNNNTGG 1 cut(s) 179
BstYI RGATCY 1 cut(s) 1887
Bsu15I ATCGAT 1 cut(s) 1690
BsuI GTATCC 1 cut(s) 1355
BsuRI GGCC 6 cut(s) 107, 1509, 1719, 1931, 2373, 2377
BsuTUI ATCGAT 1 cut(s) 1690
BtgI CCRYGG 1 cut(s) 2611
BtsCI GGATG 7 cut(s) 19, 333, 477, 1789, 2185, 2335, 2396
BtsI GCAGTG 3 cut(s) 166, 192, 2069
BtsIMutI CAGTG 7 cut(s) 166, 192, 359, 1122, 1377, 1755, 2069
BveI ACCTGC 1 cut(s) 1087
Cac8I GCNNGC 8 cut(s) 313, 486, 1446, 1507, 1707, 2375, 2379, 2388
CaiI CAGNNNCTG 1 cut(s) 2596
CfoI GCGC 5 cut(s) 1264, 1429, 2101, 2290, 2456
Cfr10I RCCGGY 3 cut(s) 1044, 1409, 2373
Cfr13I GGNCC 4 cut(s) 144, 1412, 1550, 2607
ClaI ATCGAT 1 cut(s) 1690
CsiI ACCWGGT 1 cut(s) 140
Csp6I GTAC 5 cut(s) 402, 610, 912, 2302, 2351
CspAI ACCGGT 2 cut(s) 1044, 1409
CspCI CAANNNNNGTGG 2 cut(s) 1318, 1353
CviAII CATG 4 cut(s) 689, 1139, 1763, 2566
CviQI GTAC 5 cut(s) 402, 610, 912, 2302, 2351
DpnI GATC 8 cut(s) 594, 1389, 1605, 1889, 1919, 2039, 2472, 2584
DpnII GATC 8 cut(s) 592, 1387, 1603, 1887, 1917, 2037, 2470, 2582
DriI GACNNNNNGTC 1 cut(s) 2435
EaeI YGGCCR 2 cut(s) 1929, 2375
Eam1104I CTCTTC 2 cut(s) 1457, 2638
Eam1105I GACNNNNNGTC 1 cut(s) 2435
EarI CTCTTC 2 cut(s) 1457, 2638
Ecl136II GAGCTC 4 cut(s) 301, 1655, 1709, 2362
Eco105I TACGTA 1 cut(s) 609
Eco130I CCWWGG 2 cut(s) 1926, 2253
Eco147I AGGCCT 2 cut(s) 107, 1509
Eco24I GRGCYC 5 cut(s) 285, 303, 1657, 1711, 2364
Eco32I GATATC 1 cut(s) 1632
Eco47I GGWCC 4 cut(s) 144, 1412, 1550, 2607
Eco53kI GAGCTC 4 cut(s) 301, 1655, 1709, 2362
Eco57I CTGAAG 5 cut(s) 48, 1415, 2244, 2573, 2592
Eco88I CYCGRG 2 cut(s) 154, 2602
EcoICRI GAGCTC 4 cut(s) 301, 1655, 1709, 2362
EcoNI CCTNNNNNAGG 2 cut(s) 1421, 2120
EcoO109I RGGNCCY 1 cut(s) 2607
EcoRII CCWGG 3 cut(s) 140, 1552, 2595
EcoRV GATATC 1 cut(s) 1632
EcoT14I CCWWGG 2 cut(s) 1926, 2253
EcoT38I GRGCYC 5 cut(s) 285, 303, 1657, 1711, 2364
ErhI CCWWGG 2 cut(s) 1926, 2253
FaeI CATG 4 cut(s) 692, 1142, 1766, 2569
FalI AAGNNNNNCTT 4 cut(s) 653, 685, 1704, 1736
FaqI GGGAC 5 cut(s) 583, 1398, 1472, 2045, 2578
FatI CATG 4 cut(s) 688, 1138, 1762, 2565
FauI CCCGC 2 cut(s) 277, 489
FblI GTMKAC 1 cut(s) 718
Fnu4HI GCNGC 3 cut(s) 1703, 1797, 2238
FokI GGATG 7 cut(s) 6, 320, 464, 1796, 2192, 2342, 2383
FriOI GRGCYC 5 cut(s) 285, 303, 1657, 1711, 2364
FseI GGCCGGCC 1 cut(s) 2377
Fsp4HI GCNGC 3 cut(s) 1703, 1797, 2238
FspBI CTAG 4 cut(s) 581, 782, 1304, 2507
FspI TGCGCA 1 cut(s) 2100
GlaI GCGC 5 cut(s) 1263, 1428, 2100, 2289, 2455
GluI GCNGC 3 cut(s) 1703, 1797, 2238
GsaI CCCAGC 2 cut(s) 1517, 2497
GsuI CTGGAG 1 cut(s) 386
HaeII RGCGCY 2 cut(s) 1430, 2457
HaeIII GGCC 6 cut(s) 107, 1509, 1719, 1931, 2373, 2377
HapII CCGG 5 cut(s) 1045, 1280, 1410, 2374, 2549
HhaI GCGC 5 cut(s) 1264, 1429, 2101, 2290, 2456
Hin1II CATG 4 cut(s) 692, 1142, 1766, 2569
Hin6I GCGC 5 cut(s) 1262, 1427, 2099, 2288, 2454
HinP1I GCGC 5 cut(s) 1262, 1427, 2099, 2288, 2454
HincII GTYRAC 1 cut(s) 2440
HindII GTYRAC 1 cut(s) 2440
HindIII AAGCTT 5 cut(s) 241, 644, 653, 1442, 1871
HpaII CCGG 5 cut(s) 1045, 1280, 1410, 2374, 2549
HphI GGTGA 8 cut(s) 88, 214, 258, 269, 628, 1211, 1373, 1862
Hpy99I CGWCG 1 cut(s) 2433
HpyAV CCTTC 9 cut(s) 65, 109, 464, 899, 1427, 1903, 2183, 2428, 2571
HpyCH4III ACNGT 2 cut(s) 464, 2345
HpyCH4IV ACGT 1 cut(s) 608
HpyF10VI GCNNNNNNNGC 7 cut(s) 494, 652, 1125, 1652, 2383, 2387, 2392
HpySE526I ACGT 1 cut(s) 608
Hsp92II CATG 4 cut(s) 692, 1142, 1766, 2569
HspAI GCGC 5 cut(s) 1262, 1427, 2099, 2288, 2454
Kpn2I TCCGGA 1 cut(s) 1279
KroI GCCGGC 1 cut(s) 2373
KroNI GCCGGC 1 cut(s) 2375
Kzo9I GATC 8 cut(s) 592, 1387, 1603, 1887, 1917, 2037, 2470, 2582
LmnI GCTCC 7 cut(s) 288, 1093, 1652, 2022, 2359, 2392, 2510
Lsp1109I GCAGC 3 cut(s) 1714, 1783, 2224
LweI GCATC 3 cut(s) 864, 2170, 2535
MabI ACCWGGT 1 cut(s) 140
MaeI CTAG 4 cut(s) 581, 782, 1304, 2507
MaeII ACGT 1 cut(s) 608
MaeIII GTNAC 9 cut(s) 220, 257, 431, 860, 1220, 1753, 2279, 2314, 2365
MalI GATC 8 cut(s) 594, 1389, 1605, 1889, 1919, 2039, 2472, 2584
MboI GATC 8 cut(s) 592, 1387, 1603, 1887, 1917, 2037, 2470, 2582
MboII GAAGA 9 cut(s) 83, 313, 1444, 1517, 1590, 1879, 1887, 2481, 2625
MflI RGATCY 1 cut(s) 1887
MhlI GDGCHC 9 cut(s) 161, 285, 303, 359, 499, 555, 1657, 1711, 2364
MlsI TGGCCA 1 cut(s) 1931
MluNI TGGCCA 1 cut(s) 1931
MlyI GAGTC 6 cut(s) 1217, 1494, 1559, 1761, 2411, 2594
MmeI TCCRAC 4 cut(s) 171, 1071, 1630, 2035
Mox20I TGGCCA 1 cut(s) 1931
MroI TCCGGA 1 cut(s) 1279
MroNI GCCGGC 1 cut(s) 2373
MroXI GAANNNNTTC 1 cut(s) 665
MscI TGGCCA 1 cut(s) 1931
MseI TTAA 9 cut(s) 114, 392, 758, 1020, 1028, 1185, 1287, 1467, 2055
MslI CAYNNNNRTG 3 cut(s) 897, 899, 2540
Msp20I TGGCCA 1 cut(s) 1931
MspA1I CMGCKG 1 cut(s) 2381
MspCI CTTAAG 1 cut(s) 1027
MspI CCGG 5 cut(s) 1045, 1280, 1410, 2374, 2549
MspR9I CCNGG 3 cut(s) 142, 1554, 2597
Mva1269I GAATGC 2 cut(s) 941, 2300
MvaI CCWGG 3 cut(s) 142, 1554, 2597
MwoI GCNNNNNNNGC 7 cut(s) 494, 652, 1125, 1652, 2383, 2387, 2392
NaeI GCCGGC 1 cut(s) 2375
NdeII GATC 8 cut(s) 592, 1387, 1603, 1887, 1917, 2037, 2470, 2582
NgoMIV GCCGGC 1 cut(s) 2373
NlaIII CATG 4 cut(s) 692, 1142, 1766, 2569
NlaIV GGNNCC 7 cut(s) 160, 284, 771, 1414, 1889, 2394, 2609
NmeAIII GCCGAG 1 cut(s) 1154
NmuCI GTSAC 5 cut(s) 220, 257, 1220, 1753, 2365
NsbI TGCGCA 1 cut(s) 2100
NspV TTCGAA 1 cut(s) 1898
OliI CACNNNNGTG 2 cut(s) 899, 2540
PaeR7I CTCGAG 1 cut(s) 2602
PceI AGGCCT 2 cut(s) 107, 1509
PctI GAATGC 2 cut(s) 941, 2300
PdiI GCCGGC 1 cut(s) 2375
PdmI GAANNNNTTC 1 cut(s) 665
PfoI TCCNGGA 1 cut(s) 1552
PinAI ACCGGT 2 cut(s) 1044, 1409
PkrI GCNGC 3 cut(s) 1704, 1798, 2239
PleI GAGTC 6 cut(s) 1217, 1493, 1559, 1760, 2410, 2594
PpsI GAGTC 6 cut(s) 1217, 1493, 1559, 1760, 2410, 2594
Ppu21I YACGTR 1 cut(s) 609
PpuMI RGGWCCY 1 cut(s) 2607
PsiI TTATAA 2 cut(s) 878, 1698
Psp124BI GAGCTC 4 cut(s) 303, 1657, 1711, 2364
Psp5II RGGWCCY 1 cut(s) 2607
Psp6I CCWGG 3 cut(s) 140, 1552, 2595
PspFI CCCAGC 2 cut(s) 1513, 2493
PspGI CCWGG 3 cut(s) 140, 1552, 2595
PspN4I GGNNCC 7 cut(s) 160, 284, 771, 1414, 1889, 2394, 2609
PspPI GGNCC 4 cut(s) 144, 1412, 1550, 2607
PspPPI RGGWCCY 1 cut(s) 2607
PspXI VCTCGAGB 1 cut(s) 2602
PstI CTGCAG 2 cut(s) 1801, 2045
PstNI CAGNNNCTG 1 cut(s) 2596
PsuI RGATCY 1 cut(s) 1887
PvuII CAGCTG 1 cut(s) 2381
RigI GGCCGGCC 1 cut(s) 2377
RsaI GTAC 5 cut(s) 403, 611, 913, 2303, 2352
RsaNI GTAC 5 cut(s) 402, 610, 912, 2302, 2351
RseI CAYNNNNRTG 3 cut(s) 897, 899, 2540
SacI GAGCTC 4 cut(s) 303, 1657, 1711, 2364
SaqAI TTAA 9 cut(s) 114, 392, 758, 1020, 1028, 1185, 1287, 1467, 2055
SatI GCNGC 3 cut(s) 1703, 1797, 2238
Sau3AI GATC 8 cut(s) 592, 1387, 1603, 1887, 1917, 2037, 2470, 2582
Sau96I GGNCC 4 cut(s) 144, 1412, 1550, 2607
SchI GAGTC 6 cut(s) 1217, 1494, 1559, 1761, 2411, 2594
ScrFI CCNGG 3 cut(s) 142, 1554, 2597
SduI GDGCHC 9 cut(s) 161, 285, 303, 359, 499, 555, 1657, 1711, 2364
SexAI ACCWGGT 1 cut(s) 140
SfaNI GCATC 3 cut(s) 864, 2170, 2535
SfcI CTRYAG 3 cut(s) 630, 1797, 2041
Sfr274I CTCGAG 1 cut(s) 2602
SfuI TTCGAA 1 cut(s) 1898
SinI GGWCC 4 cut(s) 144, 1412, 1550, 2607
SlaI CTCGAG 1 cut(s) 2602
SmiMI CAYNNNNRTG 3 cut(s) 897, 899, 2540
SmlI CTYRAG 8 cut(s) 451, 656, 940, 947, 1027, 1710, 2156, 2602
SmoI CTYRAG 8 cut(s) 451, 656, 940, 947, 1027, 1710, 2156, 2602
SnaBI TACGTA 1 cut(s) 609
SseBI AGGCCT 2 cut(s) 107, 1509
SsiI CCGC 4 cut(s) 270, 482, 1548, 2390
SspI AATATT 1 cut(s) 1992
SspMI CTAG 4 cut(s) 581, 782, 1304, 2507
SstI GAGCTC 4 cut(s) 303, 1657, 1711, 2364
StuI AGGCCT 2 cut(s) 107, 1509
StyD4I CCNGG 3 cut(s) 140, 1552, 2595
StyI CCWWGG 2 cut(s) 1926, 2253
TaaI ACNGT 2 cut(s) 464, 2345
TaiI ACGT 1 cut(s) 611
TaqI TCGA 8 cut(s) 447, 1563, 1690, 1898, 2217, 2469, 2603, 2625
TatI WGTACW 2 cut(s) 2301, 2350
Tru1I TTAA 9 cut(s) 114, 392, 758, 1020, 1028, 1185, 1287, 1467, 2055
Tru9I TTAA 9 cut(s) 114, 392, 758, 1020, 1028, 1185, 1287, 1467, 2055
TscAI CASTG 7 cut(s) 173, 199, 359, 1129, 1377, 1762, 2069
TseFI GTSAC 5 cut(s) 220, 257, 1220, 1753, 2365
TseI GCWGC 3 cut(s) 1702, 1796, 2237
Tsp45I GTSAC 5 cut(s) 220, 257, 1220, 1753, 2365
TspDTI ATGAA 3 cut(s) 677, 1509, 2135
TspGWI ACGGA 3 cut(s) 208, 879, 2446
TspRI CASTG 7 cut(s) 173, 199, 359, 1129, 1377, 1762, 2069
Vha464I CTTAAG 1 cut(s) 1027
VneI GTGCAC 2 cut(s) 355, 495
VpaK11BI GGWCC 4 cut(s) 144, 1412, 1550, 2607
XagI CCTNNNNNAGG 2 cut(s) 1421, 2120
XapI RAATTY 5 cut(s) 568, 1131, 1275, 1879, 2485
XcmI CCANNNNNNNNNTGG 1 cut(s) 999
XhoI CTCGAG 1 cut(s) 2602
XmiI GTMKAC 1 cut(s) 718
XmnI GAANNNNTTC 1 cut(s) 665
XspI CTAG 4 cut(s) 581, 782, 1304, 2507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.