MD12G1073500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0010860
Physical Location & Seq
Reverse (-)
8809813 .. 8811639
1827 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1073500.v1.1.491

Sequence Viewer

Length: 756 bp
ATGGCTCCGTTTCCGTCTCCAACTGAACGCATAGTTGCTTCCGCTCTGCTTCTCCTCACCGCCACACCTCCGAAGTTCTGTTTGGACGACGAACGGGAGCGAAGCAAGATCGAGAGTTGCAGAGGAAGCTCGGTTTCTGGCGATTCCATCAAGTCCTGCACTTCGTTGATCACTACCAGCGATATCTCAACGGATGAGATTCGAGCTCGTAAGATCAGGATCATGGCGCTTGCTGCTCTTCGTCGTCAGATGAATCTCAAGGTTGTTCGGAGAAGCCGCTCAAAGACTCAGACGACCTGGAAAACCATCTCTGGATCGTCCAAAGCGACGGCGTCGTTCGGATCTGAGACCACAGAGTCTTCGTGCGTGTCCACCACCTCCAGCGCGGCTTCCAGCGCGCGAAGCCGAAGCCAGCACCACGTGTCGAGATCAGGATGGAGCAGTATCAAGCTTGCGGTGGTCCGGGAGGATCCGATGAAACGAAGGCGGCTGACTTGGTCGTCTCACATGCGCCGGAAAGCCGACGCCATTCTGAAGCTCTTGTCCGGCGCCGGCTGGCTGTCTGAAGTCAAGATCCGTCAGACCATCGGTGACAGTCCTGACACCAGCAAAGCGCTCAGAATGTTGTTGAAGCTAGAGAAGGTCAAAAGAGCAGGCTCTGGAGGCCGTCACAATCCTTATGTTTACTCGGTATATATATTTATAAGTTTGTTATTCAAGTGTTGCCAAATGAATTTAAATTTTAAAATTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

252

Amino Acids

27.82

Weight (kDa)

10.48

Isoelectric Point (pI)

61.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HTH_74 PF25370 167 - 208 3.7e-22 HTH three-helical bundle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010860)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G57440 AT3G57440
fragaria_vesca FvH4_6g23771 FvH4_6g23771
malus_domestica MD12G1073500.v1.1 MD14G1067100.v1.1
prunus_persica Prupe.7G061400_v2.0.a1
pyrus_communis pycom14g05020
rosa_chinensis RchiOBHm_Chr3g0482221
rosa_laevigata RLG00000023361
rosa_multiflora Rmu_sc0017838.1_g000001
rosa_roxburghii Rroxscaffold_6G00399440 Rroxscaffold_6G00399480 Rroxscaffold_6G00399960
rosa_rugosa Rorug03G0195700
rosa_samantha Rh3AG247200 Rh3BG281800 Rh3CG277800 Rh3DG274400
rosa_wichuraiana Rw3G022110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 704
AasI GACNNNNNNGTC 2 cut(s) 355, 499
AccB1I GGYRCC 1 cut(s) 548
AccBSI CCGCTC 2 cut(s) 44, 279
AccII CGCG 3 cut(s) 386, 398, 400
AciI CCGC 6 cut(s) 42, 60, 277, 386, 455, 487
AclWI GGATC 6 cut(s) 227, 322, 349, 464, 477, 568
AcsI RAATTY 3 cut(s) 733, 739, 747
AcuI CTGAAG 2 cut(s) 554, 585
AcvI CACGTG 1 cut(s) 421
AcyI GRCGYC 3 cut(s) 332, 525, 549
AdeI CACNNNGTG 1 cut(s) 421
AfeI AGCGCT 1 cut(s) 615
AflIII ACRYGT 1 cut(s) 420
AgsI TTSAA 2 cut(s) 631, 718
AjnI CCWGG 1 cut(s) 296
AjuI GAANNNNNNNTTGG 2 cut(s) 65, 97
AluBI AGCT 5 cut(s) 129, 206, 451, 538, 634
AluI AGCT 5 cut(s) 129, 206, 451, 538, 634
Alw21I GWGCWC 1 cut(s) 208
Alw26I GTCTC 3 cut(s) 21, 341, 507
AlwI GGATC 6 cut(s) 227, 322, 349, 464, 477, 568
AlwNI CAGNNNCTG 1 cut(s) 659
Aor51HI AGCGCT 1 cut(s) 615
AoxI GGCC 1 cut(s) 664
ApeKI GCWGC 1 cut(s) 233
ApoI RAATTY 3 cut(s) 733, 739, 747
AspLEI GCGC 7 cut(s) 229, 386, 398, 400, 513, 551, 616
AspS9I GGNCC 1 cut(s) 460
AsuC2I CCSGG 1 cut(s) 464
AsuHPI GGTGA 2 cut(s) 49, 602
AvaII GGWCC 1 cut(s) 460
BamHI GGATCC 1 cut(s) 469
BanI GGYRCC 1 cut(s) 548
BanII GRGCYC 1 cut(s) 208
BbrPI CACGTG 1 cut(s) 421
BbsI GAAGAC 1 cut(s) 351
Bbv12I GWGCWC 1 cut(s) 208
BbvI GCAGC 1 cut(s) 220
BccI CCATC 4 cut(s) 155, 314, 429, 593
BceAI ACGGC 2 cut(s) 345, 651
BciT130I CCWGG 1 cut(s) 298
BclI TGATCA 1 cut(s) 168
BcnI CCSGG 1 cut(s) 464
BcoDI GTCTC 3 cut(s) 21, 341, 507
BfaI CTAG 1 cut(s) 635
BfoI RGCGCY 3 cut(s) 230, 552, 617
BisI GCNGC 4 cut(s) 234, 277, 387, 488
BlsI GCNGC 4 cut(s) 235, 278, 388, 489
Bme1390I CCNGG 2 cut(s) 298, 464
Bme18I GGWCC 1 cut(s) 460
BmgT120I GGNCC 1 cut(s) 460
BmiI GGNNCC 3 cut(s) 6, 471, 550
BmrFI CCNGG 2 cut(s) 298, 464
BpiI GAAGAC 1 cut(s) 351
BpmI CTGGAG 2 cut(s) 364, 681
BpuEI CTTGAG 1 cut(s) 242
BpuMI CCSGG 1 cut(s) 464
BsaAI YACGTR 1 cut(s) 421
BsaBI GATNNNNATC 1 cut(s) 218
BsaHI GRCGYC 3 cut(s) 332, 525, 549
BsaI GGTCTC 1 cut(s) 341
Bse118I RCCGGY 1 cut(s) 551
Bse8I GATNNNNATC 1 cut(s) 218
BseBI CCWGG 1 cut(s) 298
BseGI GGATG 2 cut(s) 199, 440
BseJI GATNNNNATC 1 cut(s) 218
BseMII CTCAG 3 cut(s) 302, 336, 631
BsePI GCGCGC 1 cut(s) 396
BseRI GAGGAG 1 cut(s) 44
BseXI GCAGC 1 cut(s) 220
BsgI GTGCAG 1 cut(s) 142
Bsh1236I CGCG 3 cut(s) 386, 398, 400
BshFI GGCC 1 cut(s) 666
BshNI GGYRCC 1 cut(s) 548
BsiHKAI GWGCWC 1 cut(s) 208
BsiSI CCGG 4 cut(s) 463, 514, 546, 552
BsmAI GTCTC 3 cut(s) 21, 341, 507
BsmBI CGTCTC 2 cut(s) 21, 507
BsnI GGCC 1 cut(s) 666
Bso31I GGTCTC 1 cut(s) 341
Bsp1286I GDGCHC 1 cut(s) 208
Bsp143I GATC 9 cut(s) 108, 168, 213, 219, 314, 341, 428, 469, 573
BspACI CCGC 6 cut(s) 42, 60, 277, 386, 455, 487
BspANI GGCC 1 cut(s) 666
BspCNI CTCAG 3 cut(s) 301, 337, 630
BspFNI CGCG 3 cut(s) 386, 398, 400
BspLI GGNNCC 3 cut(s) 6, 471, 550
BspPI GGATC 6 cut(s) 227, 322, 349, 464, 477, 568
BspQI GCTCTTC 1 cut(s) 243
BspT107I GGYRCC 1 cut(s) 548
BspTNI GGTCTC 1 cut(s) 341
BsrBI CCGCTC 2 cut(s) 44, 279
BsrFI RCCGGY 1 cut(s) 551
BssAI RCCGGY 1 cut(s) 551
BssHII GCGCGC 1 cut(s) 396
BssMI GATC 9 cut(s) 108, 168, 213, 219, 314, 341, 428, 469, 573
BssNI GRCGYC 3 cut(s) 332, 525, 549
Bst2UI CCWGG 1 cut(s) 298
Bst4CI ACNGT 1 cut(s) 596
Bst6I CTCTTC 1 cut(s) 243
BstACI GRCGYC 3 cut(s) 332, 525, 549
BstBAI YACGTR 1 cut(s) 421
BstC8I GCNNGC 7 cut(s) 231, 398, 413, 453, 553, 557, 655
BstDEI CTNAG 3 cut(s) 288, 345, 617
BstF5I GGATG 2 cut(s) 199, 440
BstFNI CGCG 3 cut(s) 386, 398, 400
BstH2I RGCGCY 3 cut(s) 230, 552, 617
BstHHI GCGC 7 cut(s) 229, 386, 398, 400, 513, 551, 616
BstKTI GATC 9 cut(s) 111, 171, 216, 222, 317, 344, 431, 472, 576
BstMAI GTCTC 3 cut(s) 21, 341, 507
BstMBI GATC 9 cut(s) 108, 168, 213, 219, 314, 341, 428, 469, 573
BstMWI GCNNNNNNNGC 5 cut(s) 126, 233, 395, 402, 663
BstNI CCWGG 1 cut(s) 298
BstNSI RCATGY 1 cut(s) 511
BstSCI CCNGG 2 cut(s) 296, 462
BstUI CGCG 3 cut(s) 386, 398, 400
BstV1I GCAGC 1 cut(s) 220
BstV2I GAAGAC 1 cut(s) 351
BstX2I RGATCY 3 cut(s) 341, 469, 573
BstYI RGATCY 3 cut(s) 341, 469, 573
BsuRI GGCC 1 cut(s) 666
BtsCI GGATG 2 cut(s) 199, 440
Cac8I GCNNGC 7 cut(s) 231, 398, 413, 453, 553, 557, 655
CaiI CAGNNNCTG 1 cut(s) 659
CfoI GCGC 7 cut(s) 229, 386, 398, 400, 513, 551, 616
Cfr10I RCCGGY 1 cut(s) 551
Cfr13I GGNCC 1 cut(s) 460
CseI GACGC 2 cut(s) 321, 533
CviAII CATG 2 cut(s) 223, 508
DdeI CTNAG 3 cut(s) 288, 345, 617
DinI GGCGCC 1 cut(s) 550
DpnI GATC 9 cut(s) 110, 170, 215, 221, 316, 343, 430, 471, 575
DpnII GATC 9 cut(s) 108, 168, 213, 219, 314, 341, 428, 469, 573
DraI TTTAAA 2 cut(s) 738, 745
DraIII CACNNNGTG 1 cut(s) 421
DrdI GACNNNNNNGTC 2 cut(s) 355, 499
DseDI GACNNNNNNGTC 2 cut(s) 355, 499
Eam1104I CTCTTC 1 cut(s) 243
EarI CTCTTC 1 cut(s) 243
Ecl136II GAGCTC 1 cut(s) 206
Eco24I GRGCYC 1 cut(s) 208
Eco31I GGTCTC 1 cut(s) 341
Eco32I GATATC 1 cut(s) 184
Eco47I GGWCC 1 cut(s) 460
Eco47III AGCGCT 1 cut(s) 615
Eco53kI GAGCTC 1 cut(s) 206
Eco57I CTGAAG 2 cut(s) 554, 585
Eco72I CACGTG 1 cut(s) 421
EcoICRI GAGCTC 1 cut(s) 206
EcoRII CCWGG 1 cut(s) 296
EcoRV GATATC 1 cut(s) 184
EcoT38I GRGCYC 1 cut(s) 208
EgeI GGCGCC 1 cut(s) 550
EheI GGCGCC 1 cut(s) 550
Esp3I CGTCTC 2 cut(s) 21, 507
FaeI CATG 2 cut(s) 226, 511
FaiI YATR 8 cut(s) 32, 224, 509, 681, 694, 696, 698, 704
FatI CATG 2 cut(s) 222, 507
FbaI TGATCA 1 cut(s) 168
Fnu4HI GCNGC 4 cut(s) 234, 277, 387, 488
FokI GGATG 2 cut(s) 206, 447
FriOI GRGCYC 1 cut(s) 208
Fsp4HI GCNGC 4 cut(s) 234, 277, 387, 488
FspBI CTAG 1 cut(s) 635
GlaI GCGC 7 cut(s) 228, 385, 397, 399, 512, 550, 615
GluI GCNGC 4 cut(s) 234, 277, 387, 488
GsuI CTGGAG 2 cut(s) 364, 681
HaeII RGCGCY 3 cut(s) 230, 552, 617
HaeIII GGCC 1 cut(s) 666
HapII CCGG 4 cut(s) 463, 514, 546, 552
HgaI GACGC 2 cut(s) 321, 533
HhaI GCGC 7 cut(s) 229, 386, 398, 400, 513, 551, 616
Hin1I GRCGYC 3 cut(s) 332, 525, 549
Hin1II CATG 2 cut(s) 226, 511
Hin6I GCGC 7 cut(s) 227, 384, 396, 398, 511, 549, 614
HinP1I GCGC 7 cut(s) 227, 384, 396, 398, 511, 549, 614
HindIII AAGCTT 1 cut(s) 449
HinfI GANTC 5 cut(s) 143, 199, 253, 286, 356
HpaII CCGG 4 cut(s) 463, 514, 546, 552
HphI GGTGA 2 cut(s) 49, 602
Hpy166II GTNNAC 2 cut(s) 372, 685
Hpy188III TCNNGA 8 cut(s) 112, 217, 312, 426, 432, 571, 599, 660
Hpy8I GTNNAC 2 cut(s) 372, 685
Hpy99I CGWCG 5 cut(s) 92, 246, 331, 337, 527
HpyAV CCTTC 2 cut(s) 477, 634
HpyCH4III ACNGT 1 cut(s) 596
HpyCH4IV ACGT 1 cut(s) 420
HpyCH4V TGCA 2 cut(s) 120, 159
HpyF10VI GCNNNNNNNGC 5 cut(s) 126, 233, 395, 402, 663
HpyF3I CTNAG 3 cut(s) 288, 345, 617
HpySE526I ACGT 1 cut(s) 420
Hsp92I GRCGYC 3 cut(s) 332, 525, 549
Hsp92II CATG 2 cut(s) 226, 511
HspAI GCGC 7 cut(s) 227, 384, 396, 398, 511, 549, 614
KasI GGCGCC 1 cut(s) 548
KroI GCCGGC 1 cut(s) 551
KroNI GCCGGC 1 cut(s) 553
Ksp22I TGATCA 1 cut(s) 168
Kzo9I GATC 9 cut(s) 108, 168, 213, 219, 314, 341, 428, 469, 573
LguI GCTCTTC 1 cut(s) 243
LmnI GCTCC 3 cut(s) 10, 97, 438
Lsp1109I GCAGC 1 cut(s) 220
MaeI CTAG 1 cut(s) 635
MaeII ACGT 1 cut(s) 420
MaeIII GTNAC 2 cut(s) 590, 668
MalI GATC 9 cut(s) 110, 170, 215, 221, 316, 343, 430, 471, 575
MbiI CCGCTC 2 cut(s) 44, 279
MboI GATC 9 cut(s) 108, 168, 213, 219, 314, 341, 428, 469, 573
MboII GAAGA 2 cut(s) 230, 351
MflI RGATCY 3 cut(s) 341, 469, 573
MhlI GDGCHC 1 cut(s) 208
MluCI AATT 3 cut(s) 733, 739, 747
Mly113I GGCGCC 1 cut(s) 549
MlyI GAGTC 2 cut(s) 280, 365
MmeI TCCRAC 1 cut(s) 44
MnlI CCTC 6 cut(s) 65, 78, 116, 388, 460, 656
MroNI GCCGGC 1 cut(s) 551
MseI TTAA 3 cut(s) 737, 744, 754
MspI CCGG 4 cut(s) 463, 514, 546, 552
MspR9I CCNGG 2 cut(s) 298, 464
MvaI CCWGG 1 cut(s) 298
MvnI CGCG 3 cut(s) 386, 398, 400
MwoI GCNNNNNNNGC 5 cut(s) 126, 233, 395, 402, 663
NaeI GCCGGC 1 cut(s) 553
NarI GGCGCC 1 cut(s) 549
NciI CCSGG 1 cut(s) 464
NdeII GATC 9 cut(s) 108, 168, 213, 219, 314, 341, 428, 469, 573
NgoMIV GCCGGC 1 cut(s) 551
NlaIII CATG 2 cut(s) 226, 511
NlaIV GGNNCC 3 cut(s) 6, 471, 550
NmuCI GTSAC 2 cut(s) 590, 668
NspI RCATGY 1 cut(s) 511
PauI GCGCGC 1 cut(s) 396
PciSI GCTCTTC 1 cut(s) 243
PcsI WCGNNNNNNNCGW 1 cut(s) 323
PdiI GCCGGC 1 cut(s) 553
PfeI GAWTC 3 cut(s) 143, 199, 253
PflFI GACNNNGTC 2 cut(s) 331, 496
PfoI TCCNGGA 1 cut(s) 462
PkrI GCNGC 4 cut(s) 235, 278, 388, 489
PleI GAGTC 2 cut(s) 280, 364
PluTI GGCGCC 1 cut(s) 552
PmaCI CACGTG 1 cut(s) 421
PmlI CACGTG 1 cut(s) 421
PpsI GAGTC 2 cut(s) 280, 364
Ppu21I YACGTR 1 cut(s) 421
PsiI TTATAA 1 cut(s) 704
Psp124BI GAGCTC 1 cut(s) 208
Psp6I CCWGG 1 cut(s) 296
PspCI CACGTG 1 cut(s) 421
PspGI CCWGG 1 cut(s) 296
PspN4I GGNNCC 3 cut(s) 6, 471, 550
PspPI GGNCC 1 cut(s) 460
PstNI CAGNNNCTG 1 cut(s) 659
PsuI RGATCY 3 cut(s) 341, 469, 573
PsyI GACNNNGTC 2 cut(s) 331, 496
PteI GCGCGC 1 cut(s) 396
SacI GAGCTC 1 cut(s) 208
SapI GCTCTTC 1 cut(s) 243
SaqAI TTAA 3 cut(s) 737, 744, 754
SatI GCNGC 4 cut(s) 234, 277, 387, 488
Sau3AI GATC 9 cut(s) 108, 168, 213, 219, 314, 341, 428, 469, 573
Sau96I GGNCC 1 cut(s) 460
SchI GAGTC 2 cut(s) 280, 365
ScrFI CCNGG 2 cut(s) 298, 464
SduI GDGCHC 1 cut(s) 208
SfoI GGCGCC 1 cut(s) 550
SinI GGWCC 1 cut(s) 460
SmiI ATTTAAAT 1 cut(s) 738
SmlI CTYRAG 1 cut(s) 257
SmoI CTYRAG 1 cut(s) 257
Sse9I AATT 3 cut(s) 733, 739, 747
SsiI CCGC 6 cut(s) 42, 60, 277, 386, 455, 487
SspDI GGCGCC 1 cut(s) 548
SspMI CTAG 1 cut(s) 635
SstI GAGCTC 1 cut(s) 208
StyD4I CCNGG 2 cut(s) 296, 462
SwaI ATTTAAAT 1 cut(s) 738
TaaI ACNGT 1 cut(s) 596
TaiI ACGT 1 cut(s) 423
TaqI TCGA 3 cut(s) 111, 202, 425
TasI AATT 3 cut(s) 733, 739, 747
TauI GCSGC 3 cut(s) 279, 389, 490
TfiI GAWTC 3 cut(s) 143, 199, 253
Tru1I TTAA 3 cut(s) 737, 744, 754
Tru9I TTAA 3 cut(s) 737, 744, 754
TseFI GTSAC 2 cut(s) 590, 668
TseI GCWGC 1 cut(s) 233
Tsp45I GTSAC 2 cut(s) 590, 668
TspDTI ATGAA 3 cut(s) 266, 491, 746
TspGWI ACGGA 2 cut(s) 206, 566
Tth111I GACNNNGTC 2 cut(s) 331, 496
VpaK11BI GGWCC 1 cut(s) 460
XapI RAATTY 3 cut(s) 733, 739, 747
XceI RCATGY 1 cut(s) 511
XspI CTAG 1 cut(s) 635
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.