pycom14g05020

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0010860
Physical Location & Seq
Reverse (-)
4837696 .. 4838676
981 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g05020.3

Sequence Viewer

Length: 342 bp
ATGTCTCAAAGCGAACGCACAGCTGCTTTGGCCCTGCATCTCCTCGCCACCAGGGATGTCTCAAAGGATGAGATTCAAGCTCATACGATGAATGTCGAGTGGTCCAGCAGGCGAAGTCGAAGCAAACGACTGCGGGTGAGGTGTTTGGTTATGGACAAGAAAGCCGAAGCCATCCTGAATCTCTTGTCACGCGACGGCTGTCTCCCTGAAGTCAAGATACGTGAGGATCTCGGTGACAATCCCGACACCAGCAAAGCACTGAGAAAGTTGCTGAGCCATGGGAAGATCAAAAGAGTAGGGGCAGGAGGGCGTCACCAGCCATATGTTTACACGATATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

114

Amino Acids

12.75

Weight (kDa)

10.08

Isoelectric Point (pI)

52.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010860)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G57440 AT3G57440
fragaria_vesca FvH4_6g23771 FvH4_6g23771
malus_domestica MD12G1073500.v1.1 MD14G1067100.v1.1
prunus_persica Prupe.7G061400_v2.0.a1
pyrus_communis pycom14g05020
rosa_chinensis RchiOBHm_Chr3g0482221
rosa_laevigata RLG00000023361
rosa_multiflora Rmu_sc0017838.1_g000001
rosa_roxburghii Rroxscaffold_6G00399440 Rroxscaffold_6G00399480 Rroxscaffold_6G00399960
rosa_rugosa Rorug03G0195700
rosa_samantha Rh3AG247200 Rh3BG281800 Rh3CG277800 Rh3DG274400
rosa_wichuraiana Rw3G022110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 192
AciI CCGC 1 cut(s) 133
AclWI GGATC 1 cut(s) 234
AcuI CTGAAG 1 cut(s) 228
AcyI GRCGYC 1 cut(s) 310
AgsI TTSAA 1 cut(s) 77
AjnI CCWGG 1 cut(s) 50
AluBI AGCT 2 cut(s) 23, 80
AluI AGCT 2 cut(s) 23, 80
Alw26I GTCTC 3 cut(s) 9, 64, 206
AlwI GGATC 1 cut(s) 234
AoxI GGCC 1 cut(s) 30
ApeKI GCWGC 1 cut(s) 23
AspS9I GGNCC 2 cut(s) 31, 102
AsuHPI GGTGA 3 cut(s) 148, 245, 305
AvaII GGWCC 1 cut(s) 102
BarI GAAGNNNNNNTAC 2 cut(s) 201, 233
BbvI GCAGC 1 cut(s) 10
BccI CCATC 1 cut(s) 179
BceAI ACGGC 1 cut(s) 211
BciT130I CCWGG 1 cut(s) 52
BcoDI GTCTC 3 cut(s) 9, 64, 206
BisI GCNGC 1 cut(s) 24
BlpI GCTNAGC 1 cut(s) 272
BlsI GCNGC 1 cut(s) 25
Bme1390I CCNGG 1 cut(s) 52
Bme18I GGWCC 1 cut(s) 102
BmgT120I GGNCC 2 cut(s) 31, 102
BmrFI CCNGG 1 cut(s) 52
BmsI GCATC 1 cut(s) 46
BoxI GACNNNNGTC 1 cut(s) 198
Bpu1102I GCTNAGC 1 cut(s) 272
BsaAI YACGTR 1 cut(s) 221
BsaHI GRCGYC 1 cut(s) 310
BsaJI CCNNGG 2 cut(s) 51, 277
BseBI CCWGG 1 cut(s) 52
BseDI CCNNGG 2 cut(s) 51, 277
BseGI GGATG 3 cut(s) 61, 73, 171
BseMII CTCAG 2 cut(s) 251, 263
BseRI GAGGAG 1 cut(s) 32
BseXI GCAGC 1 cut(s) 10
Bsh1236I CGCG 1 cut(s) 192
BshFI GGCC 1 cut(s) 32
BsmAI GTCTC 3 cut(s) 9, 64, 206
BsnI GGCC 1 cut(s) 32
Bsp143I GATC 2 cut(s) 226, 285
Bsp1720I GCTNAGC 1 cut(s) 272
Bsp19I CCATGG 1 cut(s) 277
BspACI CCGC 1 cut(s) 133
BspANI GGCC 1 cut(s) 32
BspCNI CTCAG 2 cut(s) 252, 264
BspFNI CGCG 1 cut(s) 192
BspHI TCATGA 1 cut(s) 338
BspPI GGATC 1 cut(s) 234
BssECI CCNNGG 2 cut(s) 51, 277
BssMI GATC 2 cut(s) 226, 285
BssNI GRCGYC 1 cut(s) 310
BssT1I CCWWGG 1 cut(s) 277
Bst2UI CCWGG 1 cut(s) 52
BstACI GRCGYC 1 cut(s) 310
BstBAI YACGTR 1 cut(s) 221
BstC8I GCNNGC 1 cut(s) 110
BstDEI CTNAG 2 cut(s) 260, 272
BstDSI CCRYGG 1 cut(s) 277
BstF5I GGATG 3 cut(s) 61, 73, 171
BstFNI CGCG 1 cut(s) 192
BstKTI GATC 2 cut(s) 229, 288
BstMAI GTCTC 3 cut(s) 9, 64, 206
BstMBI GATC 2 cut(s) 226, 285
BstMWI GCNNNNNNNGC 2 cut(s) 29, 316
BstNI CCWGG 1 cut(s) 52
BstPAI GACNNNNGTC 1 cut(s) 198
BstSCI CCNGG 1 cut(s) 50
BstUI CGCG 1 cut(s) 192
BstV1I GCAGC 1 cut(s) 10
BstX2I RGATCY 1 cut(s) 226
BstYI RGATCY 1 cut(s) 226
BsuRI GGCC 1 cut(s) 32
BtgI CCRYGG 1 cut(s) 277
BtsCI GGATG 3 cut(s) 61, 73, 171
BtsIMutI CAGTG 1 cut(s) 257
Cac8I GCNNGC 1 cut(s) 110
CciI TCATGA 1 cut(s) 338
Cfr13I GGNCC 2 cut(s) 31, 102
CseI GACGC 1 cut(s) 299
CviAII CATG 2 cut(s) 278, 339
CviJI RGCY 8 cut(s) 23, 32, 80, 164, 170, 198, 276, 319
CviKI_1 RGCY 8 cut(s) 23, 32, 80, 164, 170, 198, 276, 319
DdeI CTNAG 2 cut(s) 260, 272
DpnI GATC 2 cut(s) 228, 287
DpnII GATC 2 cut(s) 226, 285
Eco130I CCWWGG 1 cut(s) 277
Eco32I GATATC 1 cut(s) 336
Eco47I GGWCC 1 cut(s) 102
Eco57I CTGAAG 1 cut(s) 228
EcoRII CCWGG 1 cut(s) 50
EcoRV GATATC 1 cut(s) 336
EcoT14I CCWWGG 1 cut(s) 277
ErhI CCWWGG 1 cut(s) 277
FaeI CATG 2 cut(s) 281, 342
FaiI YATR 6 cut(s) 84, 152, 279, 322, 324, 340
FatI CATG 2 cut(s) 277, 338
FauI CCCGC 1 cut(s) 126
FauNDI CATATG 1 cut(s) 322
Fnu4HI GCNGC 1 cut(s) 24
FokI GGATG 3 cut(s) 68, 80, 158
Fsp4HI GCNGC 1 cut(s) 24
GluI GCNGC 1 cut(s) 24
HaeIII GGCC 1 cut(s) 32
HgaI GACGC 1 cut(s) 299
Hin1I GRCGYC 1 cut(s) 310
Hin1II CATG 2 cut(s) 281, 342
HinfI GANTC 2 cut(s) 73, 178
HphI GGTGA 3 cut(s) 148, 245, 305
Hpy166II GTNNAC 1 cut(s) 328
Hpy188III TCNNGA 4 cut(s) 175, 214, 242, 339
Hpy8I GTNNAC 1 cut(s) 328
Hpy99I CGWCG 1 cut(s) 197
HpyCH4IV ACGT 1 cut(s) 220
HpyCH4V TGCA 1 cut(s) 37
HpyF10VI GCNNNNNNNGC 2 cut(s) 29, 316
HpyF3I CTNAG 2 cut(s) 260, 272
HpySE526I ACGT 1 cut(s) 220
Hsp92I GRCGYC 1 cut(s) 310
Hsp92II CATG 2 cut(s) 281, 342
Kzo9I GATC 2 cut(s) 226, 285
Lsp1109I GCAGC 1 cut(s) 10
LweI GCATC 1 cut(s) 46
MaeII ACGT 1 cut(s) 220
MaeIII GTNAC 3 cut(s) 186, 233, 311
MalI GATC 2 cut(s) 228, 287
MboI GATC 2 cut(s) 226, 285
MboII GAAGA 1 cut(s) 295
MflI RGATCY 1 cut(s) 226
MnlI CCTC 4 cut(s) 53, 132, 217, 299
MspA1I CMGCKG 1 cut(s) 23
MspR9I CCNGG 1 cut(s) 52
MvaI CCWGG 1 cut(s) 52
MvnI CGCG 1 cut(s) 192
MwoI GCNNNNNNNGC 2 cut(s) 29, 316
NcoI CCATGG 1 cut(s) 277
NdeI CATATG 1 cut(s) 322
NdeII GATC 2 cut(s) 226, 285
NlaIII CATG 2 cut(s) 281, 342
NmuCI GTSAC 3 cut(s) 186, 233, 311
PagI TCATGA 1 cut(s) 338
PcsI WCGNNNNNNNCGW 1 cut(s) 124
PfeI GAWTC 2 cut(s) 73, 178
PkrI GCNGC 1 cut(s) 25
Ppu21I YACGTR 1 cut(s) 221
PshAI GACNNNNGTC 1 cut(s) 198
Psp6I CCWGG 1 cut(s) 50
PspGI CCWGG 1 cut(s) 50
PspPI GGNCC 2 cut(s) 31, 102
PsuI RGATCY 1 cut(s) 226
PvuII CAGCTG 1 cut(s) 23
SatI GCNGC 1 cut(s) 24
Sau3AI GATC 2 cut(s) 226, 285
Sau96I GGNCC 2 cut(s) 31, 102
ScrFI CCNGG 1 cut(s) 52
SetI ASST 4 cut(s) 25, 82, 143, 223
SfaNI GCATC 1 cut(s) 46
SinI GGWCC 1 cut(s) 102
SsiI CCGC 1 cut(s) 133
StyD4I CCNGG 1 cut(s) 50
StyI CCWWGG 1 cut(s) 277
TaiI ACGT 1 cut(s) 223
TaqI TCGA 2 cut(s) 96, 118
TfiI GAWTC 2 cut(s) 73, 178
TscAI CASTG 1 cut(s) 264
TseFI GTSAC 3 cut(s) 186, 233, 311
TseI GCWGC 1 cut(s) 23
Tsp45I GTSAC 3 cut(s) 186, 233, 311
TspDTI ATGAA 1 cut(s) 104
TspRI CASTG 1 cut(s) 264
VpaK11BI GGWCC 1 cut(s) 102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.