MD12G1074200.v1.1

Protein N-terminal glutamine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0014502
Physical Location & Seq
Reverse (-)
8851667 .. 8855630
3964 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1074200.v1.1.491

Sequence Viewer

Length: 678 bp
ATGACGACGTCGAAGTTGGAAGTAAGCGAGAGCTCCTGCGACATCTCTCGGTTCCATCACACCCCATTTTACTGTGAGGAGAATGTGTACTTGCTTTGCAAGGAGCTGTGCAAAAAGGGCATCGCAGATGCACAGGGTTCTGATCTTTTCGTTGTTTTCATTTCCAATGACAAGAAACAGATCCCGTTGTGGCGCCAGAAGGCCAGCAATCGAGCGGATGGGGTTGTTCTGTGGGATTATCATGTCATTTGCGTGCAGAAAAAAGGCAGTGGTGACACACCCGCAACACATTTAGTGTGGGATTTGGATTCGAGTCTTGCATTTCCTTGTCCTTTAGCAACCTATGTCTTGGAAACGTTCTGCCCCTCGTTTCAGACCTTTTCTGAATTGCGAAGGTGTTTCCGGATTGTGCATGCCCCAATATTCCTTCGTTTCTTTGCCTCTGATAGAAGACACATGAAAGATTCTAATGGAAATTGGCTTCGTCAACCTCCTCTCTATCAGCCTATTGTTGCGCAGGATGGGACTGTACACAACCTGGATGGTTACTTTCAGATCCATGCTACAGATGCGGTGACAGGAACAGGAGTTGACGTGACCAATGCAGTTTTTACTGAAAAGCTTGGTGTGGTCGTAACAGAAAATCAGCTGGAGGAGTTCTTTTCCCAGATTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nt_Gln_amidase

Protein Analysis

226

Amino Acids

25.52

Weight (kDa)

5.88

Isoelectric Point (pI)

39.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nt_Gln_amidase PF09764 20 - 221 1.3e-65 N-terminal glutamine amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014502)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41760
fragaria_vesca FvH4_6g23850
malus_domestica MD12G1074200.v1.1
prunus_persica Prupe.7G059700_v2.0.a1
pyrus_communis pycom12g06400
rosa_chinensis RchiOBHm_Chr3g0482531
rosa_laevigata RLG00000023347
rosa_multiflora Rmu_sc0002969.1_g000011 Rmu_sc0005613.1_g000003
rosa_roxburghii Rroxscaffold_6G00399310
rosa_rugosa Rorug03G0196700
rosa_samantha Rh3AG248200 Rh3BG283200 Rh3CG279800 Rh3DG276100
rosa_wichuraiana Rw0G013140 Rw3G022350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 11
Acc16I TGCGCA 1 cut(s) 516
AccB1I GGYRCC 1 cut(s) 192
AccBSI CCGCTC 1 cut(s) 215
AccIII TCCGGA 1 cut(s) 402
AciI CCGC 3 cut(s) 215, 282, 572
AclI AACGTT 1 cut(s) 356
AclWI GGATC 2 cut(s) 175, 550
AcyI GRCGYC 2 cut(s) 8, 193
AfaI GTAC 2 cut(s) 89, 531
AjiI CACGTC 1 cut(s) 595
AjnI CCWGG 1 cut(s) 537
AluBI AGCT 4 cut(s) 33, 106, 622, 649
AluI AGCT 4 cut(s) 33, 106, 622, 649
Alw21I GWGCWC 1 cut(s) 35
AlwI GGATC 2 cut(s) 175, 550
Aor13HI TCCGGA 1 cut(s) 402
AoxI GGCC 1 cut(s) 201
AspLEI GCGC 2 cut(s) 195, 517
AsuHPI GGTGA 2 cut(s) 284, 586
BanI GGYRCC 1 cut(s) 192
BanII GRGCYC 1 cut(s) 35
BbsI GAAGAC 1 cut(s) 457
Bbv12I GWGCWC 1 cut(s) 35
BccI CCATC 4 cut(s) 63, 212, 515, 536
BciT130I CCWGG 1 cut(s) 539
BfmI CTRYAG 1 cut(s) 564
BfoI RGCGCY 1 cut(s) 196
Bme1390I CCNGG 1 cut(s) 539
BmgBI CACGTC 1 cut(s) 595
BmiI GGNNCC 2 cut(s) 53, 194
BmrFI CCNGG 1 cut(s) 539
BmsI GCATC 3 cut(s) 118, 129, 559
BpiI GAAGAC 1 cut(s) 457
BpmI CTGGAG 1 cut(s) 671
BsaHI GRCGYC 2 cut(s) 8, 193
BsaWI WCCGGW 1 cut(s) 402
BsaXI ACNNNNNCTCC 4 cut(s) 71, 101, 579, 609
BseAI TCCGGA 1 cut(s) 402
BseBI CCWGG 1 cut(s) 539
BseGI GGATG 3 cut(s) 223, 526, 547
BseRI GAGGAG 3 cut(s) 92, 483, 668
BsgI GTGCAG 1 cut(s) 275
BshFI GGCC 1 cut(s) 203
BshNI GGYRCC 1 cut(s) 192
BsiHKAI GWGCWC 1 cut(s) 35
BsiSI CCGG 1 cut(s) 403
BslFI GGGAC 1 cut(s) 538
BsmFI GGGAC 1 cut(s) 538
BsnI GGCC 1 cut(s) 203
Bsp1286I GDGCHC 1 cut(s) 35
Bsp13I TCCGGA 1 cut(s) 402
Bsp1407I TGTACA 1 cut(s) 529
Bsp143I GATC 3 cut(s) 142, 180, 555
BspACI CCGC 3 cut(s) 215, 282, 572
BspANI GGCC 1 cut(s) 203
BspEI TCCGGA 1 cut(s) 402
BspLI GGNNCC 2 cut(s) 53, 194
BspPI GGATC 2 cut(s) 175, 550
BspT107I GGYRCC 1 cut(s) 192
BsrBI CCGCTC 1 cut(s) 215
BsrGI TGTACA 1 cut(s) 529
BssMI GATC 3 cut(s) 142, 180, 555
BssNI GRCGYC 2 cut(s) 8, 193
Bst2UI CCWGG 1 cut(s) 539
Bst4CI ACNGT 2 cut(s) 74, 529
BstACI GRCGYC 2 cut(s) 8, 193
BstAUI TGTACA 1 cut(s) 529
BstC8I GCNNGC 3 cut(s) 205, 254, 414
BstF5I GGATG 3 cut(s) 223, 526, 547
BstH2I RGCGCY 1 cut(s) 196
BstHHI GCGC 2 cut(s) 195, 517
BstKTI GATC 3 cut(s) 145, 183, 558
BstMBI GATC 3 cut(s) 142, 180, 555
BstMWI GCNNNNNNNGC 2 cut(s) 117, 569
BstNI CCWGG 1 cut(s) 539
BstNSI RCATGY 1 cut(s) 416
BstSCI CCNGG 1 cut(s) 537
BstSFI CTRYAG 1 cut(s) 564
BstV2I GAAGAC 1 cut(s) 457
BstX2I RGATCY 2 cut(s) 180, 555
BstYI RGATCY 2 cut(s) 180, 555
BsuRI GGCC 1 cut(s) 203
BtgZI GCGATG 1 cut(s) 106
BtrI CACGTC 1 cut(s) 595
BtsCI GGATG 3 cut(s) 223, 526, 547
BtsI GCAGTG 1 cut(s) 274
BtsIMutI CAGTG 1 cut(s) 274
Cac8I GCNNGC 3 cut(s) 205, 254, 414
CfoI GCGC 2 cut(s) 195, 517
Csp6I GTAC 2 cut(s) 88, 530
CviAII CATG 4 cut(s) 242, 413, 457, 560
CviJI RGCY 7 cut(s) 33, 106, 203, 481, 505, 622, 649
CviKI_1 RGCY 7 cut(s) 33, 106, 203, 481, 505, 622, 649
CviQI GTAC 2 cut(s) 88, 530
DinI GGCGCC 1 cut(s) 194
DpnI GATC 3 cut(s) 144, 182, 557
DpnII GATC 3 cut(s) 142, 180, 555
Ecl136II GAGCTC 1 cut(s) 33
Eco24I GRGCYC 1 cut(s) 35
Eco53kI GAGCTC 1 cut(s) 33
EcoICRI GAGCTC 1 cut(s) 33
EcoRII CCWGG 1 cut(s) 537
EcoT38I GRGCYC 1 cut(s) 35
EgeI GGCGCC 1 cut(s) 194
EheI GGCGCC 1 cut(s) 194
FaeI CATG 4 cut(s) 245, 416, 460, 563
FaiI YATR 5 cut(s) 243, 345, 414, 458, 561
FaqI GGGAC 1 cut(s) 538
FatI CATG 4 cut(s) 241, 412, 456, 559
FauI CCCGC 1 cut(s) 289
FokI GGATG 3 cut(s) 230, 533, 554
FriOI GRGCYC 1 cut(s) 35
FspI TGCGCA 1 cut(s) 516
GlaI GCGC 2 cut(s) 194, 516
GsuI CTGGAG 1 cut(s) 671
HaeII RGCGCY 1 cut(s) 196
HaeIII GGCC 1 cut(s) 203
HapII CCGG 1 cut(s) 403
HhaI GCGC 2 cut(s) 195, 517
Hin1I GRCGYC 2 cut(s) 8, 193
Hin1II CATG 4 cut(s) 245, 416, 460, 563
Hin6I GCGC 2 cut(s) 193, 515
HinP1I GCGC 2 cut(s) 193, 515
HincII GTYRAC 2 cut(s) 488, 592
HindII GTYRAC 2 cut(s) 488, 592
HindIII AAGCTT 1 cut(s) 620
HinfI GANTC 4 cut(s) 308, 313, 464, 670
HpaII CCGG 1 cut(s) 403
HphI GGTGA 2 cut(s) 284, 586
Hpy166II GTNNAC 4 cut(s) 88, 488, 532, 592
Hpy188I TCNGA 5 cut(s) 142, 375, 385, 445, 555
Hpy188III TCNNGA 1 cut(s) 403
Hpy8I GTNNAC 4 cut(s) 88, 488, 532, 592
Hpy99I CGWCG 2 cut(s) 10, 13
HpyAV CCTTC 3 cut(s) 193, 387, 437
HpyCH4III ACNGT 2 cut(s) 74, 529
HpyCH4IV ACGT 3 cut(s) 8, 356, 594
HpyCH4V TGCA 7 cut(s) 99, 111, 131, 256, 320, 412, 605
HpyF10VI GCNNNNNNNGC 2 cut(s) 117, 569
HpySE526I ACGT 3 cut(s) 8, 356, 594
Hsp92I GRCGYC 2 cut(s) 8, 193
Hsp92II CATG 4 cut(s) 245, 416, 460, 563
HspAI GCGC 2 cut(s) 193, 515
KasI GGCGCC 1 cut(s) 192
Kpn2I TCCGGA 1 cut(s) 402
Kzo9I GATC 3 cut(s) 142, 180, 555
LmnI GCTCC 2 cut(s) 38, 103
LweI GCATC 3 cut(s) 118, 129, 559
MaeII ACGT 3 cut(s) 8, 356, 594
MaeIII GTNAC 5 cut(s) 272, 545, 574, 595, 634
MalI GATC 3 cut(s) 144, 182, 557
MbiI CCGCTC 1 cut(s) 215
MboI GATC 3 cut(s) 142, 180, 555
MboII GAAGA 1 cut(s) 462
MflI RGATCY 2 cut(s) 180, 555
MhlI GDGCHC 1 cut(s) 35
MluCI AATT 2 cut(s) 386, 475
Mly113I GGCGCC 1 cut(s) 193
MlyI GAGTC 1 cut(s) 322
MnlI CCTC 6 cut(s) 70, 376, 451, 501, 504, 646
MroI TCCGGA 1 cut(s) 402
MseI TTAA 1 cut(s) 676
MslI CAYNNNNRTG 1 cut(s) 251
MspA1I CMGCKG 1 cut(s) 649
MspI CCGG 1 cut(s) 403
MspR9I CCNGG 1 cut(s) 539
MvaI CCWGG 1 cut(s) 539
MwoI GCNNNNNNNGC 2 cut(s) 117, 569
NarI GGCGCC 1 cut(s) 193
NdeII GATC 3 cut(s) 142, 180, 555
NlaIII CATG 4 cut(s) 245, 416, 460, 563
NlaIV GGNNCC 2 cut(s) 53, 194
NmuCI GTSAC 3 cut(s) 272, 574, 595
NsbI TGCGCA 1 cut(s) 516
NspI RCATGY 1 cut(s) 416
PaeI GCATGC 1 cut(s) 416
PfeI GAWTC 3 cut(s) 308, 464, 670
PflFI GACNNNGTC 1 cut(s) 7
PleI GAGTC 1 cut(s) 321
PluTI GGCGCC 1 cut(s) 196
PpsI GAGTC 1 cut(s) 321
Psp124BI GAGCTC 1 cut(s) 35
Psp1406I AACGTT 1 cut(s) 356
Psp6I CCWGG 1 cut(s) 537
PspGI CCWGG 1 cut(s) 537
PspN4I GGNNCC 2 cut(s) 53, 194
PsuI RGATCY 2 cut(s) 180, 555
PsyI GACNNNGTC 1 cut(s) 7
PvuII CAGCTG 1 cut(s) 649
RsaI GTAC 2 cut(s) 89, 531
RsaNI GTAC 2 cut(s) 88, 530
RseI CAYNNNNRTG 1 cut(s) 251
SacI GAGCTC 1 cut(s) 35
SaqAI TTAA 1 cut(s) 676
Sau3AI GATC 3 cut(s) 142, 180, 555
SchI GAGTC 1 cut(s) 322
ScrFI CCNGG 1 cut(s) 539
SduI GDGCHC 1 cut(s) 35
SfaNI GCATC 3 cut(s) 118, 129, 559
SfcI CTRYAG 1 cut(s) 564
SfoI GGCGCC 1 cut(s) 194
SmiMI CAYNNNNRTG 1 cut(s) 251
SphI GCATGC 1 cut(s) 416
Sse9I AATT 2 cut(s) 386, 475
SsiI CCGC 3 cut(s) 215, 282, 572
SspDI GGCGCC 1 cut(s) 192
SspI AATATT 1 cut(s) 423
SstI GAGCTC 1 cut(s) 35
StyD4I CCNGG 1 cut(s) 537
TaaI ACNGT 2 cut(s) 74, 529
TaiI ACGT 3 cut(s) 11, 359, 597
TaqI TCGA 3 cut(s) 11, 211, 311
TasI AATT 2 cut(s) 386, 475
TatI WGTACW 2 cut(s) 87, 529
TfiI GAWTC 3 cut(s) 308, 464, 670
Tru1I TTAA 1 cut(s) 676
Tru9I TTAA 1 cut(s) 676
TscAI CASTG 1 cut(s) 274
TseFI GTSAC 3 cut(s) 272, 574, 595
Tsp45I GTSAC 3 cut(s) 272, 574, 595
TspDTI ATGAA 2 cut(s) 148, 473
TspRI CASTG 1 cut(s) 274
Tth111I GACNNNGTC 1 cut(s) 7
XceI RCATGY 1 cut(s) 416
ZraI GACGTC 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.