Rh3BG283200

Protein N-terminal glutamine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0014502
Physical Location & Seq
Reverse (-)
28133179 .. 28137358
4180 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG283200.1

Sequence Viewer

Length: 708 bp
ATGGAAACGAGGCAGGGAGAGCGGAGAATGAAGATGACGACCTCAAAGTTGGAAGCGAGCACTGTGGACGTTTCGCAGTTCCAGCACACCCCTTTTTACTGTGAGGAGAATGTGTACTTGCTTTGCAAGGAATTGAGCAAAAAGGGAATCGCAGATGAAAAAAGCTCTGATCTTTTTGTGGTTTTGATTTCCAATGAAAAGAAACAGATACCATTGTGGCATCAAAAGGCGAGCAACCGAGCAGATGGAGTTGTTCTCTGGGATTATCATGTCATTTGCGTACAGAGAAAAGTTGGTGGAGGTGACTCACCCTCGCAGCATTTAGTGTGGGATTTGGATTCAACTCTTTCGTTTCCTTGTGATTTAGCAACTTATGTCTCCGAAACCATCCGCCCCACATTTCAGACCTTCCCTGAATTCCAGAGATATTTCCGGATTGTGCATGCCCCAATATTCCTCCGTAACTTTGCATCTGATAGAAGGCACATGAAAGATTCTCTTGGCGAATGGCTTCATCAACCTCCTCTATATGAGCCGATTGTTTCTGAAGATGGAACTGTACATAACCTGAATGACTACCTTGACATCAGGGCTACAGATGTTGTGAAGGAGGCAGGTCATGATATGACCGATCCAGTTTTTACCCAAAAGCTTGGCGTGGTCGTAGGTGAAAATCAACTGGAGGAATTCTTTTCCCAAATTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nt_Gln_amidase

Protein Analysis

235

Amino Acids

27.09

Weight (kDa)

5.47

Isoelectric Point (pI)

40.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nt_Gln_amidase PF09764 29 - 231 1.4e-66 N-terminal glutamine amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014502)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41760
fragaria_vesca FvH4_6g23850
malus_domestica MD12G1074200.v1.1
prunus_persica Prupe.7G059700_v2.0.a1
pyrus_communis pycom12g06400
rosa_chinensis RchiOBHm_Chr3g0482531
rosa_laevigata RLG00000023347
rosa_multiflora Rmu_sc0002969.1_g000011 Rmu_sc0005613.1_g000003
rosa_roxburghii Rroxscaffold_6G00399310
rosa_rugosa Rorug03G0196700
rosa_samantha Rh3AG248200 Rh3BG283200 Rh3CG279800 Rh3DG276100
rosa_wichuraiana Rw0G013140 Rw3G022350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 605
AccBSI CCGCTC 1 cut(s) 22
AccIII TCCGGA 1 cut(s) 432
AciI CCGC 2 cut(s) 22, 391
AclWI GGATC 1 cut(s) 626
AcsI RAATTY 2 cut(s) 416, 686
AcuI CTGAAG 1 cut(s) 567
AfaI GTAC 3 cut(s) 116, 282, 561
AgsI TTSAA 1 cut(s) 342
AluBI AGCT 2 cut(s) 165, 652
AluI AGCT 2 cut(s) 165, 652
Alw21I GWGCWC 1 cut(s) 62
Alw26I GTCTC 1 cut(s) 382
AlwI GGATC 1 cut(s) 626
Aor13HI TCCGGA 1 cut(s) 432
ApeKI GCWGC 1 cut(s) 316
ApoI RAATTY 2 cut(s) 416, 686
Asp700I GAANNNNTTC 1 cut(s) 510
AsuHPI GGTGA 3 cut(s) 300, 314, 680
BaeI ACNNNNGTAYC 2 cut(s) 200, 233
Bbv12I GWGCWC 1 cut(s) 62
BbvI GCAGC 1 cut(s) 328
BccI CCATC 3 cut(s) 239, 395, 545
BcoDI GTCTC 1 cut(s) 382
BfmI CTRYAG 1 cut(s) 594
BfuAI ACCTGC 1 cut(s) 605
BisI GCNGC 1 cut(s) 317
BlsI GCNGC 1 cut(s) 318
BmsI GCATC 2 cut(s) 229, 479
BplI GAGNNNNNCTC 2 cut(s) 240, 272
BpmI CTGGAG 1 cut(s) 701
BsaWI WCCGGW 1 cut(s) 432
BsaXI ACNNNNNCTCC 2 cut(s) 98, 128
Bse1I ACTGG 2 cut(s) 635, 684
BseAI TCCGGA 1 cut(s) 432
BseGI GGATG 1 cut(s) 387
BseNI ACTGG 2 cut(s) 635, 684
BseRI GAGGAG 2 cut(s) 119, 513
BseXI GCAGC 1 cut(s) 328
BsiHKAI GWGCWC 1 cut(s) 62
BsiSI CCGG 1 cut(s) 433
BsmAI GTCTC 1 cut(s) 382
Bsp1286I GDGCHC 1 cut(s) 62
Bsp13I TCCGGA 1 cut(s) 432
Bsp1407I TGTACA 1 cut(s) 559
Bsp143I GATC 2 cut(s) 169, 631
BspACI CCGC 2 cut(s) 22, 391
BspEI TCCGGA 1 cut(s) 432
BspHI TCATGA 1 cut(s) 619
BspMI ACCTGC 1 cut(s) 605
BspPI GGATC 1 cut(s) 626
BsrBI CCGCTC 1 cut(s) 22
BsrGI TGTACA 1 cut(s) 559
BsrI ACTGG 2 cut(s) 635, 684
BssMI GATC 2 cut(s) 169, 631
Bst4CI ACNGT 3 cut(s) 64, 101, 559
BstAUI TGTACA 1 cut(s) 559
BstC8I GCNNGC 3 cut(s) 58, 232, 444
BstF5I GGATG 1 cut(s) 387
BstKTI GATC 2 cut(s) 172, 634
BstMAI GTCTC 1 cut(s) 382
BstMBI GATC 2 cut(s) 169, 631
BstMWI GCNNNNNNNGC 2 cut(s) 19, 82
BstNSI RCATGY 1 cut(s) 446
BstSFI CTRYAG 1 cut(s) 594
BstV1I GCAGC 1 cut(s) 328
BstXI CCANNNNNNTGG 1 cut(s) 653
BtsCI GGATG 1 cut(s) 387
BtsIMutI CAGTG 1 cut(s) 60
BveI ACCTGC 1 cut(s) 605
Cac8I GCNNGC 3 cut(s) 58, 232, 444
CciI TCATGA 1 cut(s) 619
Csp6I GTAC 3 cut(s) 115, 281, 560
CviAII CATG 4 cut(s) 269, 443, 487, 620
CviJI RGCY 5 cut(s) 165, 511, 535, 593, 652
CviKI_1 RGCY 5 cut(s) 165, 511, 535, 593, 652
CviQI GTAC 3 cut(s) 115, 281, 560
DpnI GATC 2 cut(s) 171, 633
DpnII GATC 2 cut(s) 169, 631
EciI GGCGGA 1 cut(s) 380
Eco57I CTGAAG 1 cut(s) 567
EcoRI GAATTC 2 cut(s) 416, 686
FaeI CATG 4 cut(s) 272, 446, 490, 623
FaiI YATR 9 cut(s) 270, 375, 444, 488, 529, 531, 564, 621, 626
FalI AAGNNNNNCTT 2 cut(s) 483, 515
FatI CATG 4 cut(s) 268, 442, 486, 619
Fnu4HI GCNGC 1 cut(s) 317
FokI GGATG 1 cut(s) 374
Fsp4HI GCNGC 1 cut(s) 317
GluI GCNGC 1 cut(s) 317
GsuI CTGGAG 1 cut(s) 701
HapII CCGG 1 cut(s) 433
Hin1II CATG 4 cut(s) 272, 446, 490, 623
HindIII AAGCTT 1 cut(s) 650
HinfI GANTC 4 cut(s) 147, 305, 338, 494
HpaII CCGG 1 cut(s) 433
HphI GGTGA 3 cut(s) 300, 314, 680
Hpy166II GTNNAC 2 cut(s) 67, 115
Hpy188I TCNGA 5 cut(s) 169, 382, 405, 475, 547
Hpy188III TCNNGA 3 cut(s) 421, 433, 620
Hpy8I GTNNAC 2 cut(s) 67, 115
HpyAV CCTTC 3 cut(s) 418, 474, 601
HpyCH4III ACNGT 3 cut(s) 64, 101, 559
HpyCH4IV ACGT 1 cut(s) 69
HpyCH4V TGCA 3 cut(s) 126, 442, 470
HpyF10VI GCNNNNNNNGC 2 cut(s) 19, 82
HpySE526I ACGT 1 cut(s) 69
Hsp92II CATG 4 cut(s) 272, 446, 490, 623
Kpn2I TCCGGA 1 cut(s) 432
Kzo9I GATC 2 cut(s) 169, 631
Lsp1109I GCAGC 1 cut(s) 328
LweI GCATC 2 cut(s) 229, 479
MaeII ACGT 1 cut(s) 69
MaeIII GTNAC 2 cut(s) 302, 461
MalI GATC 2 cut(s) 171, 633
MbiI CCGCTC 1 cut(s) 22
MboI GATC 2 cut(s) 169, 631
MboII GAAGA 2 cut(s) 43, 560
MhlI GDGCHC 1 cut(s) 62
MluCI AATT 4 cut(s) 131, 416, 686, 699
MlyI GAGTC 1 cut(s) 299
MmeI TCCRAC 1 cut(s) 30
MroI TCCGGA 1 cut(s) 432
MroXI GAANNNNTTC 1 cut(s) 510
MspI CCGG 1 cut(s) 433
MwoI GCNNNNNNNGC 2 cut(s) 19, 82
NdeII GATC 2 cut(s) 169, 631
NlaIII CATG 4 cut(s) 272, 446, 490, 623
NmuCI GTSAC 1 cut(s) 302
NspI RCATGY 1 cut(s) 446
PaeI GCATGC 1 cut(s) 446
PagI TCATGA 1 cut(s) 619
PdmI GAANNNNTTC 1 cut(s) 510
PfeI GAWTC 3 cut(s) 147, 338, 494
PkrI GCNGC 1 cut(s) 318
PleI GAGTC 1 cut(s) 299
PpsI GAGTC 1 cut(s) 299
RsaI GTAC 3 cut(s) 116, 282, 561
RsaNI GTAC 3 cut(s) 115, 281, 560
SatI GCNGC 1 cut(s) 317
Sau3AI GATC 2 cut(s) 169, 631
SchI GAGTC 1 cut(s) 299
SduI GDGCHC 1 cut(s) 62
SfaNI GCATC 2 cut(s) 229, 479
SfcI CTRYAG 1 cut(s) 594
SphI GCATGC 1 cut(s) 446
Sse9I AATT 4 cut(s) 131, 416, 686, 699
SsiI CCGC 2 cut(s) 22, 391
SspI AATATT 1 cut(s) 453
TaaI ACNGT 3 cut(s) 64, 101, 559
TaiI ACGT 1 cut(s) 72
TaqII GACCGA 1 cut(s) 644
TasI AATT 4 cut(s) 131, 416, 686, 699
TatI WGTACW 2 cut(s) 114, 559
TfiI GAWTC 3 cut(s) 147, 338, 494
TscAI CASTG 1 cut(s) 67
TseFI GTSAC 1 cut(s) 302
TseI GCWGC 1 cut(s) 316
Tsp45I GTSAC 1 cut(s) 302
TspDTI ATGAA 5 cut(s) 44, 171, 210, 503, 503
TspGWI ACGGA 1 cut(s) 449
TspRI CASTG 1 cut(s) 67
XapI RAATTY 2 cut(s) 416, 686
XceI RCATGY 1 cut(s) 446
XmnI GAANNNNTTC 1 cut(s) 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.