pycom12g06400

Protein N-terminal glutamine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0014502
Physical Location & Seq
Reverse (-)
6527783 .. 6530150
2368 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g06400.1

Sequence Viewer

Length: 648 bp
ATGACGACGTCAAAGTTGGAAGAAAGCGAGAGCTCCTGCGACATCTCTCAGTTCCATCACACCCCATTTTACTGTGAGGAGAATGTGTACTTGCTTTGCAAGGAGCTGTGCAAAAGGGGCATCGCAGATGCACAGGGTTCTGATCTTTTCATCCCGTTGTGGCGCCAGAAGGCCAGCAATCGAGCGGATGGGGTTGTTCTGTGGGATTATCATGTCATTTGCGTGCAGAAAAAAGGCAGTGGTGACACACCCGCAACACATTTAGTGTGGGATTTGGATTCGAGTCTTGCATTTCCTTGTCCTTTAGCAACCTATGTCTTGGAAACGTTCTGCCCCTCATTTCAGACCTTTGCTGAATTGCGAAGGTGTTTCCGGATTGTGCATGCCCCAATATTCCTTCGTTTCTTTGCTTCTGATAGAAGACACATGAAAGATTCTAATGGAAATTGGCTTCGTCAACCTCCTCTCTATCAGCCTATTGTTGCGCAGGATGGGACTGTACACAACCTGGATGGTTACTTTCAGATCCGTGCTACAGATGCGGTGACAAGAACAGGAGTTGACGTGACCAATGCAGTTTTTACTGAAAAGCTTGGTGTGGTCGTAACAGAAAATCAGCTGGAGGGGTTCTTTTCCCACATTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nt_Gln_amidase

Protein Analysis

216

Amino Acids

24.43

Weight (kDa)

5.88

Isoelectric Point (pI)

43.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nt_Gln_amidase PF09764 20 - 211 1.5e-53 N-terminal glutamine amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014502)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41760
fragaria_vesca FvH4_6g23850
malus_domestica MD12G1074200.v1.1
prunus_persica Prupe.7G059700_v2.0.a1
pyrus_communis pycom12g06400
rosa_chinensis RchiOBHm_Chr3g0482531
rosa_laevigata RLG00000023347
rosa_multiflora Rmu_sc0002969.1_g000011 Rmu_sc0005613.1_g000003
rosa_roxburghii Rroxscaffold_6G00399310
rosa_rugosa Rorug03G0196700
rosa_samantha Rh3AG248200 Rh3BG283200 Rh3CG279800 Rh3DG276100
rosa_wichuraiana Rw0G013140 Rw3G022350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 11
Acc16I TGCGCA 1 cut(s) 486
AccB1I GGYRCC 1 cut(s) 162
AccBSI CCGCTC 1 cut(s) 185
AccIII TCCGGA 1 cut(s) 372
AciI CCGC 3 cut(s) 185, 252, 542
AclI AACGTT 1 cut(s) 326
AclWI GGATC 1 cut(s) 520
AcyI GRCGYC 2 cut(s) 8, 163
AfaI GTAC 2 cut(s) 89, 501
AjiI CACGTC 1 cut(s) 565
AjnI CCWGG 1 cut(s) 507
AluBI AGCT 4 cut(s) 33, 106, 592, 619
AluI AGCT 4 cut(s) 33, 106, 592, 619
Alw21I GWGCWC 1 cut(s) 35
AlwI GGATC 1 cut(s) 520
Aor13HI TCCGGA 1 cut(s) 372
AoxI GGCC 1 cut(s) 171
AspLEI GCGC 2 cut(s) 165, 487
AsuHPI GGTGA 2 cut(s) 254, 556
BanI GGYRCC 1 cut(s) 162
BanII GRGCYC 1 cut(s) 35
BbsI GAAGAC 1 cut(s) 427
Bbv12I GWGCWC 1 cut(s) 35
BccI CCATC 4 cut(s) 63, 182, 485, 506
BciT130I CCWGG 1 cut(s) 509
BfmI CTRYAG 1 cut(s) 534
BfoI RGCGCY 1 cut(s) 166
Bme1390I CCNGG 1 cut(s) 509
BmgBI CACGTC 1 cut(s) 565
BmiI GGNNCC 1 cut(s) 164
BmrFI CCNGG 1 cut(s) 509
BmsI GCATC 3 cut(s) 118, 129, 529
BpiI GAAGAC 1 cut(s) 427
BpmI CTGGAG 1 cut(s) 641
BsaHI GRCGYC 2 cut(s) 8, 163
BsaWI WCCGGW 1 cut(s) 372
BsaXI ACNNNNNCTCC 4 cut(s) 71, 101, 549, 579
BseAI TCCGGA 1 cut(s) 372
BseBI CCWGG 1 cut(s) 509
BseGI GGATG 4 cut(s) 150, 193, 496, 517
BseMII CTCAG 1 cut(s) 62
BseRI GAGGAG 2 cut(s) 92, 453
BsgI GTGCAG 1 cut(s) 245
BshFI GGCC 1 cut(s) 173
BshNI GGYRCC 1 cut(s) 162
BsiHKAI GWGCWC 1 cut(s) 35
BsiSI CCGG 1 cut(s) 373
BslFI GGGAC 1 cut(s) 508
BsmFI GGGAC 1 cut(s) 508
BsnI GGCC 1 cut(s) 173
Bsp1286I GDGCHC 1 cut(s) 35
Bsp13I TCCGGA 1 cut(s) 372
Bsp1407I TGTACA 1 cut(s) 499
Bsp143I GATC 2 cut(s) 142, 525
BspACI CCGC 3 cut(s) 185, 252, 542
BspANI GGCC 1 cut(s) 173
BspCNI CTCAG 1 cut(s) 61
BspEI TCCGGA 1 cut(s) 372
BspLI GGNNCC 1 cut(s) 164
BspPI GGATC 1 cut(s) 520
BspT107I GGYRCC 1 cut(s) 162
BsrBI CCGCTC 1 cut(s) 185
BsrGI TGTACA 1 cut(s) 499
BssMI GATC 2 cut(s) 142, 525
BssNI GRCGYC 2 cut(s) 8, 163
Bst2UI CCWGG 1 cut(s) 509
Bst4CI ACNGT 2 cut(s) 74, 499
BstACI GRCGYC 2 cut(s) 8, 163
BstAUI TGTACA 1 cut(s) 499
BstC8I GCNNGC 3 cut(s) 175, 224, 384
BstDEI CTNAG 1 cut(s) 48
BstF5I GGATG 4 cut(s) 150, 193, 496, 517
BstH2I RGCGCY 1 cut(s) 166
BstHHI GCGC 2 cut(s) 165, 487
BstKTI GATC 2 cut(s) 145, 528
BstMBI GATC 2 cut(s) 142, 525
BstMWI GCNNNNNNNGC 2 cut(s) 117, 539
BstNI CCWGG 1 cut(s) 509
BstNSI RCATGY 1 cut(s) 386
BstSCI CCNGG 1 cut(s) 507
BstSFI CTRYAG 1 cut(s) 534
BstV2I GAAGAC 1 cut(s) 427
BstX2I RGATCY 1 cut(s) 525
BstYI RGATCY 1 cut(s) 525
BsuRI GGCC 1 cut(s) 173
BtgZI GCGATG 1 cut(s) 106
BtrI CACGTC 1 cut(s) 565
BtsCI GGATG 4 cut(s) 150, 193, 496, 517
BtsI GCAGTG 1 cut(s) 244
BtsIMutI CAGTG 1 cut(s) 244
Cac8I GCNNGC 3 cut(s) 175, 224, 384
CfoI GCGC 2 cut(s) 165, 487
Csp6I GTAC 2 cut(s) 88, 500
CviAII CATG 3 cut(s) 212, 383, 427
CviJI RGCY 7 cut(s) 33, 106, 173, 451, 475, 592, 619
CviKI_1 RGCY 7 cut(s) 33, 106, 173, 451, 475, 592, 619
CviQI GTAC 2 cut(s) 88, 500
DdeI CTNAG 1 cut(s) 48
DinI GGCGCC 1 cut(s) 164
DpnI GATC 2 cut(s) 144, 527
DpnII GATC 2 cut(s) 142, 525
Ecl136II GAGCTC 1 cut(s) 33
Eco24I GRGCYC 1 cut(s) 35
Eco53kI GAGCTC 1 cut(s) 33
EcoICRI GAGCTC 1 cut(s) 33
EcoRII CCWGG 1 cut(s) 507
EcoT38I GRGCYC 1 cut(s) 35
EgeI GGCGCC 1 cut(s) 164
EheI GGCGCC 1 cut(s) 164
FaeI CATG 3 cut(s) 215, 386, 430
FaiI YATR 4 cut(s) 213, 315, 384, 428
FaqI GGGAC 1 cut(s) 508
FatI CATG 3 cut(s) 211, 382, 426
FauI CCCGC 1 cut(s) 259
FokI GGATG 4 cut(s) 137, 200, 503, 524
FriOI GRGCYC 1 cut(s) 35
FspI TGCGCA 1 cut(s) 486
GlaI GCGC 2 cut(s) 164, 486
GsuI CTGGAG 1 cut(s) 641
HaeII RGCGCY 1 cut(s) 166
HaeIII GGCC 1 cut(s) 173
HapII CCGG 1 cut(s) 373
HhaI GCGC 2 cut(s) 165, 487
Hin1I GRCGYC 2 cut(s) 8, 163
Hin1II CATG 3 cut(s) 215, 386, 430
Hin6I GCGC 2 cut(s) 163, 485
HinP1I GCGC 2 cut(s) 163, 485
HincII GTYRAC 2 cut(s) 458, 562
HindII GTYRAC 2 cut(s) 458, 562
HindIII AAGCTT 1 cut(s) 590
HinfI GANTC 3 cut(s) 278, 283, 434
HpaII CCGG 1 cut(s) 373
HphI GGTGA 2 cut(s) 254, 556
Hpy166II GTNNAC 4 cut(s) 88, 458, 502, 562
Hpy188I TCNGA 4 cut(s) 142, 345, 415, 525
Hpy188III TCNNGA 1 cut(s) 373
Hpy8I GTNNAC 4 cut(s) 88, 458, 502, 562
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 3 cut(s) 163, 357, 407
HpyCH4III ACNGT 2 cut(s) 74, 499
HpyCH4IV ACGT 3 cut(s) 8, 326, 564
HpyCH4V TGCA 7 cut(s) 99, 111, 131, 226, 290, 382, 575
HpyF10VI GCNNNNNNNGC 2 cut(s) 117, 539
HpyF3I CTNAG 1 cut(s) 48
HpySE526I ACGT 3 cut(s) 8, 326, 564
Hsp92I GRCGYC 2 cut(s) 8, 163
Hsp92II CATG 3 cut(s) 215, 386, 430
HspAI GCGC 2 cut(s) 163, 485
KasI GGCGCC 1 cut(s) 162
Kpn2I TCCGGA 1 cut(s) 372
Kzo9I GATC 2 cut(s) 142, 525
LmnI GCTCC 2 cut(s) 38, 103
LweI GCATC 3 cut(s) 118, 129, 529
MaeII ACGT 3 cut(s) 8, 326, 564
MaeIII GTNAC 5 cut(s) 242, 515, 544, 565, 604
MalI GATC 2 cut(s) 144, 527
MbiI CCGCTC 1 cut(s) 185
MboI GATC 2 cut(s) 142, 525
MboII GAAGA 2 cut(s) 32, 432
MflI RGATCY 1 cut(s) 525
MhlI GDGCHC 1 cut(s) 35
MluCI AATT 2 cut(s) 356, 445
Mly113I GGCGCC 1 cut(s) 163
MlyI GAGTC 1 cut(s) 292
MnlI CCTC 5 cut(s) 70, 346, 471, 474, 616
MroI TCCGGA 1 cut(s) 372
MseI TTAA 1 cut(s) 646
MslI CAYNNNNRTG 1 cut(s) 221
MspA1I CMGCKG 1 cut(s) 619
MspI CCGG 1 cut(s) 373
MspR9I CCNGG 1 cut(s) 509
MvaI CCWGG 1 cut(s) 509
MwoI GCNNNNNNNGC 2 cut(s) 117, 539
NarI GGCGCC 1 cut(s) 163
NdeII GATC 2 cut(s) 142, 525
NlaIII CATG 3 cut(s) 215, 386, 430
NlaIV GGNNCC 1 cut(s) 164
NmuCI GTSAC 3 cut(s) 242, 544, 565
NsbI TGCGCA 1 cut(s) 486
NspI RCATGY 1 cut(s) 386
PaeI GCATGC 1 cut(s) 386
PfeI GAWTC 2 cut(s) 278, 434
PflFI GACNNNGTC 1 cut(s) 7
PleI GAGTC 1 cut(s) 291
PluTI GGCGCC 1 cut(s) 166
PpsI GAGTC 1 cut(s) 291
Psp124BI GAGCTC 1 cut(s) 35
Psp1406I AACGTT 1 cut(s) 326
Psp6I CCWGG 1 cut(s) 507
PspGI CCWGG 1 cut(s) 507
PspN4I GGNNCC 1 cut(s) 164
PsuI RGATCY 1 cut(s) 525
PsyI GACNNNGTC 1 cut(s) 7
PvuII CAGCTG 1 cut(s) 619
RsaI GTAC 2 cut(s) 89, 501
RsaNI GTAC 2 cut(s) 88, 500
RseI CAYNNNNRTG 1 cut(s) 221
SacI GAGCTC 1 cut(s) 35
SaqAI TTAA 1 cut(s) 646
Sau3AI GATC 2 cut(s) 142, 525
SchI GAGTC 1 cut(s) 292
ScrFI CCNGG 1 cut(s) 509
SduI GDGCHC 1 cut(s) 35
SfaNI GCATC 3 cut(s) 118, 129, 529
SfcI CTRYAG 1 cut(s) 534
SfoI GGCGCC 1 cut(s) 164
SmiMI CAYNNNNRTG 1 cut(s) 221
SphI GCATGC 1 cut(s) 386
Sse9I AATT 2 cut(s) 356, 445
SsiI CCGC 3 cut(s) 185, 252, 542
SspDI GGCGCC 1 cut(s) 162
SspI AATATT 1 cut(s) 393
SstI GAGCTC 1 cut(s) 35
StyD4I CCNGG 1 cut(s) 507
TaaI ACNGT 2 cut(s) 74, 499
TaiI ACGT 3 cut(s) 11, 329, 567
TaqI TCGA 2 cut(s) 181, 281
TasI AATT 2 cut(s) 356, 445
TatI WGTACW 2 cut(s) 87, 499
TfiI GAWTC 2 cut(s) 278, 434
Tru1I TTAA 1 cut(s) 646
Tru9I TTAA 1 cut(s) 646
TscAI CASTG 1 cut(s) 244
TseFI GTSAC 3 cut(s) 242, 544, 565
Tsp45I GTSAC 3 cut(s) 242, 544, 565
TspDTI ATGAA 2 cut(s) 139, 443
TspGWI ACGGA 1 cut(s) 518
TspRI CASTG 1 cut(s) 244
Tth111I GACNNNGTC 1 cut(s) 7
XceI RCATGY 1 cut(s) 386
ZraI GACGTC 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.