Rroxscaffold_6G00399310

Protein N-terminal glutamine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0014502
Physical Location & Seq
Forward (+)
21247281 .. 21251861
4581 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00399310.1

Sequence Viewer

Length: 681 bp
ATGACGACCTCAAAATTGGAAGCGAGCACTAGCACTGTGGACGCTTCGCAGTTCCAGCACACCCCTTTTTACTGTGAGGAGAATGTGTACTTGCTTTGCAAGGAATTGAGCGAAAAGGGAATCGCAGATGAAAAAAGCTCCGATCTTTTTGTGGTTTTGATTTCCAATGAAAAGAAACAGATACCATTGTGGCATCAAAAGGCGAGCAACCGAGCAGATGGAGTTGTTCTCTGGGATTATCACGTCATTTGCGTACAGAGAAAAGTTGGTGGAGGTGACTCACCCTCGCAGCATTTAGTGTGGGATTTGGATTCAACTCTTTCGTTTCCTTGTGCTTTAGCAACTTATGTCTCTGAAACCATCCGCCCCACATTTCAGACCTTCCCTGAATTCCAGAGGTATTTCCGGATTGTGCATGCCCCAATATTCCTCCGTAACTTTGCATCTGATAGAAGGCACATGAAAGATTCTCTTGGCGAATGGCTTCATCAGCCTCCTCTGTATCTGCCGATTGTTTCTGAAGATGGAACTGTACATAACCTGAATGACTACCTTGACATCAGGGCTACAGATGTTGTGAAGGAGACAGGTCATGATATGACCGATCCAGTTTTTACCCAAAAGCTTGGTGTGGTTGTAGGTGAAAATCAACTGGAGGAATTCTTTTCCCAAATTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nt_Gln_amidase

Protein Analysis

226

Amino Acids

25.81

Weight (kDa)

5.21

Isoelectric Point (pI)

37.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nt_Gln_amidase PF09764 20 - 222 1.3e-65 N-terminal glutamine amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014502)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41760
fragaria_vesca FvH4_6g23850
malus_domestica MD12G1074200.v1.1
prunus_persica Prupe.7G059700_v2.0.a1
pyrus_communis pycom12g06400
rosa_chinensis RchiOBHm_Chr3g0482531
rosa_laevigata RLG00000023347
rosa_multiflora Rmu_sc0002969.1_g000011 Rmu_sc0005613.1_g000003
rosa_roxburghii Rroxscaffold_6G00399310
rosa_rugosa Rorug03G0196700
rosa_samantha Rh3AG248200 Rh3BG283200 Rh3CG279800 Rh3DG276100
rosa_wichuraiana Rw0G013140 Rw3G022350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 405
AciI CCGC 1 cut(s) 364
AclWI GGATC 1 cut(s) 599
AcsI RAATTY 3 cut(s) 389, 659, 672
AcuI CTGAAG 1 cut(s) 540
AfaI GTAC 3 cut(s) 89, 255, 534
AgsI TTSAA 1 cut(s) 315
AjiI CACGTC 1 cut(s) 244
AluBI AGCT 2 cut(s) 138, 625
AluI AGCT 2 cut(s) 138, 625
Alw21I GWGCWC 1 cut(s) 29
Alw26I GTCTC 2 cut(s) 355, 578
AlwI GGATC 1 cut(s) 599
Aor13HI TCCGGA 1 cut(s) 405
ApeKI GCWGC 1 cut(s) 289
ApoI RAATTY 3 cut(s) 389, 659, 672
Asp700I GAANNNNTTC 1 cut(s) 483
AsuHPI GGTGA 3 cut(s) 273, 287, 653
BaeI ACNNNNGTAYC 2 cut(s) 173, 206
Bbv12I GWGCWC 1 cut(s) 29
BbvI GCAGC 1 cut(s) 301
BccI CCATC 3 cut(s) 212, 368, 518
BcoDI GTCTC 2 cut(s) 355, 578
BfaI CTAG 1 cut(s) 30
BfmI CTRYAG 1 cut(s) 567
BisI GCNGC 1 cut(s) 290
BlsI GCNGC 1 cut(s) 291
BmgBI CACGTC 1 cut(s) 244
BmsI GCATC 2 cut(s) 202, 452
BplI GAGNNNNNCTC 2 cut(s) 213, 245
BpmI CTGGAG 1 cut(s) 674
BsaWI WCCGGW 1 cut(s) 405
BsaXI ACNNNNNCTCC 2 cut(s) 71, 101
Bse1I ACTGG 2 cut(s) 608, 657
BseAI TCCGGA 1 cut(s) 405
BseGI GGATG 1 cut(s) 360
BseNI ACTGG 2 cut(s) 608, 657
BseRI GAGGAG 2 cut(s) 92, 486
BseXI GCAGC 1 cut(s) 301
BsiHKAI GWGCWC 1 cut(s) 29
BsiSI CCGG 1 cut(s) 406
BsmAI GTCTC 2 cut(s) 355, 578
Bsp1286I GDGCHC 1 cut(s) 29
Bsp13I TCCGGA 1 cut(s) 405
Bsp1407I TGTACA 1 cut(s) 532
Bsp143I GATC 2 cut(s) 142, 604
BspACI CCGC 1 cut(s) 364
BspEI TCCGGA 1 cut(s) 405
BspHI TCATGA 1 cut(s) 592
BspPI GGATC 1 cut(s) 599
BsrGI TGTACA 1 cut(s) 532
BsrI ACTGG 2 cut(s) 608, 657
BssMI GATC 2 cut(s) 142, 604
Bst4CI ACNGT 3 cut(s) 37, 74, 532
BstAUI TGTACA 1 cut(s) 532
BstC8I GCNNGC 3 cut(s) 25, 205, 417
BstF5I GGATG 1 cut(s) 360
BstKTI GATC 2 cut(s) 145, 607
BstMAI GTCTC 2 cut(s) 355, 578
BstMBI GATC 2 cut(s) 142, 604
BstMWI GCNNNNNNNGC 2 cut(s) 55, 490
BstNSI RCATGY 1 cut(s) 419
BstSFI CTRYAG 1 cut(s) 567
BstV1I GCAGC 1 cut(s) 301
BstXI CCANNNNNNTGG 1 cut(s) 626
BtrI CACGTC 1 cut(s) 244
BtsCI GGATG 1 cut(s) 360
BtsIMutI CAGTG 1 cut(s) 33
Cac8I GCNNGC 3 cut(s) 25, 205, 417
CciI TCATGA 1 cut(s) 592
CseI GACGC 1 cut(s) 50
Csp6I GTAC 3 cut(s) 88, 254, 533
CviAII CATG 3 cut(s) 416, 460, 593
CviJI RGCY 5 cut(s) 138, 484, 493, 566, 625
CviKI_1 RGCY 5 cut(s) 138, 484, 493, 566, 625
CviQI GTAC 3 cut(s) 88, 254, 533
DpnI GATC 2 cut(s) 144, 606
DpnII GATC 2 cut(s) 142, 604
EciI GGCGGA 1 cut(s) 353
Eco57I CTGAAG 1 cut(s) 540
EcoRI GAATTC 2 cut(s) 389, 659
FaeI CATG 3 cut(s) 419, 463, 596
FaiI YATR 6 cut(s) 348, 417, 461, 537, 594, 599
FalI AAGNNNNNCTT 2 cut(s) 456, 488
FatI CATG 3 cut(s) 415, 459, 592
Fnu4HI GCNGC 1 cut(s) 290
FokI GGATG 1 cut(s) 347
Fsp4HI GCNGC 1 cut(s) 290
FspBI CTAG 1 cut(s) 30
GluI GCNGC 1 cut(s) 290
GsuI CTGGAG 1 cut(s) 674
HapII CCGG 1 cut(s) 406
HgaI GACGC 1 cut(s) 50
Hin1II CATG 3 cut(s) 419, 463, 596
HindIII AAGCTT 1 cut(s) 623
HinfI GANTC 4 cut(s) 120, 278, 311, 467
HpaII CCGG 1 cut(s) 406
HphI GGTGA 3 cut(s) 273, 287, 653
Hpy166II GTNNAC 2 cut(s) 40, 88
Hpy188I TCNGA 5 cut(s) 142, 355, 378, 448, 520
Hpy188III TCNNGA 3 cut(s) 394, 406, 593
Hpy8I GTNNAC 2 cut(s) 40, 88
HpyAV CCTTC 3 cut(s) 391, 447, 574
HpyCH4III ACNGT 3 cut(s) 37, 74, 532
HpyCH4IV ACGT 1 cut(s) 243
HpyCH4V TGCA 3 cut(s) 99, 415, 443
HpyF10VI GCNNNNNNNGC 2 cut(s) 55, 490
HpySE526I ACGT 1 cut(s) 243
Hsp92II CATG 3 cut(s) 419, 463, 596
Kpn2I TCCGGA 1 cut(s) 405
Kzo9I GATC 2 cut(s) 142, 604
LmnI GCTCC 1 cut(s) 143
Lsp1109I GCAGC 1 cut(s) 301
LweI GCATC 2 cut(s) 202, 452
MaeI CTAG 1 cut(s) 30
MaeII ACGT 1 cut(s) 243
MaeIII GTNAC 2 cut(s) 275, 434
MalI GATC 2 cut(s) 144, 606
MboI GATC 2 cut(s) 142, 604
MboII GAAGA 1 cut(s) 533
MhlI GDGCHC 1 cut(s) 29
MluCI AATT 5 cut(s) 14, 104, 389, 659, 672
MlyI GAGTC 1 cut(s) 272
MnlI CCTC 9 cut(s) 19, 70, 266, 295, 390, 440, 504, 507, 649
MroI TCCGGA 1 cut(s) 405
MroXI GAANNNNTTC 1 cut(s) 483
MspI CCGG 1 cut(s) 406
MwoI GCNNNNNNNGC 2 cut(s) 55, 490
NdeII GATC 2 cut(s) 142, 604
NlaIII CATG 3 cut(s) 419, 463, 596
NmuCI GTSAC 1 cut(s) 275
NspI RCATGY 1 cut(s) 419
PaeI GCATGC 1 cut(s) 419
PagI TCATGA 1 cut(s) 592
PcsI WCGNNNNNNNCGW 1 cut(s) 249
PdmI GAANNNNTTC 1 cut(s) 483
PfeI GAWTC 3 cut(s) 120, 311, 467
PkrI GCNGC 1 cut(s) 291
PleI GAGTC 1 cut(s) 272
PpsI GAGTC 1 cut(s) 272
RsaI GTAC 3 cut(s) 89, 255, 534
RsaNI GTAC 3 cut(s) 88, 254, 533
SatI GCNGC 1 cut(s) 290
Sau3AI GATC 2 cut(s) 142, 604
SchI GAGTC 1 cut(s) 272
SduI GDGCHC 1 cut(s) 29
SfaNI GCATC 2 cut(s) 202, 452
SfcI CTRYAG 1 cut(s) 567
SphI GCATGC 1 cut(s) 419
Sse9I AATT 5 cut(s) 14, 104, 389, 659, 672
SsiI CCGC 1 cut(s) 364
SspI AATATT 1 cut(s) 426
SspMI CTAG 1 cut(s) 30
TaaI ACNGT 3 cut(s) 37, 74, 532
TaiI ACGT 1 cut(s) 246
TaqII GACCGA 1 cut(s) 617
TasI AATT 5 cut(s) 14, 104, 389, 659, 672
TatI WGTACW 2 cut(s) 87, 532
TfiI GAWTC 3 cut(s) 120, 311, 467
TscAI CASTG 1 cut(s) 40
TseFI GTSAC 1 cut(s) 275
TseI GCWGC 1 cut(s) 289
Tsp45I GTSAC 1 cut(s) 275
TspDTI ATGAA 4 cut(s) 144, 183, 476, 476
TspGWI ACGGA 1 cut(s) 422
TspRI CASTG 1 cut(s) 40
XapI RAATTY 3 cut(s) 389, 659, 672
XceI RCATGY 1 cut(s) 419
XmnI GAANNNNTTC 1 cut(s) 483
XspI CTAG 1 cut(s) 30
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.