Rorug03G0196700

Protein N-terminal glutamine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0014502
Physical Location & Seq
Reverse (-)
17280472 .. 17283192
2721 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0196700.1

Sequence Viewer

Length: 975 bp
ATGGCATCCATGGCTACCTGCTTCACTCTTCCACCAATCCCTCGTCATGTTCTCGTTTTCAAAGCTGCACTTTTACCTAATCGTTCCCAAACCTCCAATTTCCCGGGCGCTGCCTCGAGGGTTCCACAATCTAGGGCTGCCGCGCCGGTTGTCAGAGCCAGCACTGCAGCGATCAGGCCCGGCGGTGCCGTCGAGAGCGATAAGTTACCTTCCGACGTGAGAAAACGAGCAATGGACGCTGTGGACGCTTGTGGAAGAAGAGTCTCCGATGAAGGCAATGTACTCTATGTTTTTCCCAGAGATTATCGAGCAAAACTTGTAGGCAGATCTTTCATAATAAGAGTTGAGCCGCTGCTTGAGAAGGCAAAGGCTGGGGCTGAGTATTTAGCAAGGGTTTCATTTGGAACTACTCTGATTGCTTCCATTATTCTTGTGTTCGCTGCAATTCTTGTTGCCCTTATAGCCATTGCCTTAAATGAAAATGTTGATGTCATCCCGTCGACGTCTTCAACTTCTTCTTTCGCTAGTGGAGGAAGCTCATATCGGACGAGTTATGATTATTCAATTGATGTTTTTCCCTCGTGTCGGACTGAAGTGTATCGGAAGCAAAAAGAAAAACCACAACCAATTGTTGGGACGTTGAAATTTGGTGAATCTTTTTTCTCATTTGTATTTGGCGATGGTGATCCGAATCGAGGAATCGAAGAAAAGAGATGGAAGCTGACCCAGAGGTCATCTTCTTCGCCTTCTTCCAAGTGCGCAGGGGCAACTGCCAAACCTCGCCACCCCCTGAATCCGCCCCTGGCGGCGAAAACACTCGACGGCAAGGCCCAGCGGCGATTCGGACTCCGATCGACAAGGCCCAGCTCCGATTCCGATCGACAAGGACCTGCTCCGATTCCGATCGACAAGGCCCAGCCTCGATTCCGAACCCAACGCCTCCAATTCCGGTCAGCTTCACGACGGAGGCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nt_Gln_amidase

Protein Analysis

324

Amino Acids

35.32

Weight (kDa)

10.57

Isoelectric Point (pI)

55.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014502)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41760
fragaria_vesca FvH4_6g23850
malus_domestica MD12G1074200.v1.1
prunus_persica Prupe.7G059700_v2.0.a1
pyrus_communis pycom12g06400
rosa_chinensis RchiOBHm_Chr3g0482531
rosa_laevigata RLG00000023347
rosa_multiflora Rmu_sc0002969.1_g000011 Rmu_sc0005613.1_g000003
rosa_roxburghii Rroxscaffold_6G00399310
rosa_rugosa Rorug03G0196700
rosa_samantha Rh3AG248200 Rh3BG283200 Rh3CG279800 Rh3DG276100
rosa_wichuraiana Rw0G013140 Rw3G022350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 730
AatII GACGTC 1 cut(s) 506
AbsI CCTCGAGG 1 cut(s) 115
Acc16I TGCGCA 1 cut(s) 760
Acc36I ACCTGC 2 cut(s) 26, 898
AccB1I GGYRCC 1 cut(s) 185
AccB7I CCANNNNNTGG 1 cut(s) 632
AccI GTMKAC 1 cut(s) 500
AccII CGCG 1 cut(s) 143
AciI CCGC 6 cut(s) 141, 183, 350, 797, 806, 835
AclWI GGATC 1 cut(s) 680
AcsI RAATTY 1 cut(s) 644
AcuI CTGAAG 1 cut(s) 612
AcyI GRCGYC 1 cut(s) 503
AfaI GTAC 1 cut(s) 282
AfiI CCNNNNNNNGG 3 cut(s) 585, 632, 695
AgsI TTSAA 4 cut(s) 61, 510, 564, 643
AjiI CACGTC 1 cut(s) 217
AjnI CCWGG 1 cut(s) 801
AluBI AGCT 5 cut(s) 65, 537, 721, 867, 956
AluI AGCT 5 cut(s) 65, 537, 721, 867, 956
Alw26I GTCTC 1 cut(s) 268
AlwI GGATC 1 cut(s) 680
Ama87I CYCGRG 2 cut(s) 103, 115
AoxI GGCC 4 cut(s) 176, 828, 860, 912
ApeKI GCWGC 6 cut(s) 65, 110, 137, 167, 352, 440
ApoI RAATTY 1 cut(s) 644
AspLEI GCGC 3 cut(s) 110, 145, 761
AspS9I GGNCC 5 cut(s) 177, 829, 861, 887, 913
AsuC2I CCSGG 3 cut(s) 104, 105, 180
AsuHPI GGTGA 2 cut(s) 662, 695
AvaI CYCGRG 2 cut(s) 103, 115
AvaII GGWCC 1 cut(s) 887
BanI GGYRCC 1 cut(s) 185
BarI GAAGNNNNNNTAC 2 cut(s) 264, 296
BauI CACGAG 1 cut(s) 580
BbsI GAAGAC 1 cut(s) 498
BbvI GCAGC 6 cut(s) 52, 97, 124, 179, 339, 427
BccI CCATC 2 cut(s) 674, 708
BceAI ACGGC 2 cut(s) 173, 838
BciT130I CCWGG 1 cut(s) 803
BcnI CCSGG 3 cut(s) 104, 105, 180
BcoDI GTCTC 1 cut(s) 268
BfaI CTAG 2 cut(s) 132, 525
BfmI CTRYAG 1 cut(s) 165
BfoI RGCGCY 1 cut(s) 111
BfuAI ACCTGC 2 cut(s) 26, 898
BglII AGATCT 1 cut(s) 326
Bme1390I CCNGG 4 cut(s) 104, 105, 180, 803
Bme18I GGWCC 1 cut(s) 887
BmeT110I CYCGRG 2 cut(s) 103, 115
BmgBI CACGTC 1 cut(s) 217
BmgT120I GGNCC 5 cut(s) 177, 829, 861, 887, 913
BmiI GGNNCC 2 cut(s) 123, 187
BmrFI CCNGG 4 cut(s) 104, 105, 180, 803
BmsI GCATC 1 cut(s) 14
BpiI GAAGAC 1 cut(s) 498
BpuEI CTTGAG 1 cut(s) 377
BpuMI CCSGG 3 cut(s) 104, 105, 180
BsaBI GATNNNNATC 4 cut(s) 684, 690, 876, 902
BsaHI GRCGYC 1 cut(s) 503
BsaJI CCNNGG 3 cut(s) 9, 103, 801
BsaWI WCCGGW 1 cut(s) 948
Bsc4I CCNNNNNNNGG 3 cut(s) 585, 632, 695
Bse118I RCCGGY 1 cut(s) 145
Bse3DI GCAATG 3 cut(s) 237, 283, 465
Bse8I GATNNNNATC 4 cut(s) 684, 690, 876, 902
BseBI CCWGG 1 cut(s) 803
BseDI CCNNGG 3 cut(s) 9, 103, 801
BseGI GGATG 2 cut(s) 5, 492
BseJI GATNNNNATC 4 cut(s) 684, 690, 876, 902
BseLI CCNNNNNNNGG 3 cut(s) 585, 632, 695
BseMI GCAATG 3 cut(s) 237, 283, 465
BseMII CTCAG 1 cut(s) 369
BseXI GCAGC 6 cut(s) 52, 97, 124, 179, 339, 427
BseYI CCCAGC 4 cut(s) 371, 831, 863, 915
BsgI GTGCAG 1 cut(s) 51
Bsh1236I CGCG 1 cut(s) 143
Bsh1285I CGRYCG 3 cut(s) 854, 880, 906
BshFI GGCC 4 cut(s) 178, 830, 862, 914
BshNI GGYRCC 1 cut(s) 185
BsiEI CGRYCG 3 cut(s) 854, 880, 906
BsiHKCI CYCGRG 2 cut(s) 103, 115
BsiSI CCGG 4 cut(s) 104, 146, 180, 949
BslFI GGGAC 1 cut(s) 649
BslI CCNNNNNNNGG 3 cut(s) 585, 632, 695
BsmAI GTCTC 1 cut(s) 268
BsmFI GGGAC 1 cut(s) 649
BsnI GGCC 4 cut(s) 178, 830, 862, 914
BsoBI CYCGRG 2 cut(s) 103, 115
Bsp143I GATC 6 cut(s) 171, 326, 685, 851, 877, 903
Bsp19I CCATGG 1 cut(s) 9
BspACI CCGC 6 cut(s) 141, 183, 350, 797, 806, 835
BspANI GGCC 4 cut(s) 178, 830, 862, 914
BspCNI CTCAG 1 cut(s) 370
BspFNI CGCG 1 cut(s) 143
BspLI GGNNCC 2 cut(s) 123, 187
BspMAI CTGCAG 1 cut(s) 169
BspMI ACCTGC 2 cut(s) 26, 898
BspPI GGATC 1 cut(s) 680
BspT107I GGYRCC 1 cut(s) 185
BsrDI GCAATG 3 cut(s) 237, 283, 465
BsrFI RCCGGY 1 cut(s) 145
BssAI RCCGGY 1 cut(s) 145
BssECI CCNNGG 3 cut(s) 9, 103, 801
BssMI GATC 6 cut(s) 171, 326, 685, 851, 877, 903
BssNI GRCGYC 1 cut(s) 503
BssSI CACGAG 1 cut(s) 580
BssT1I CCWWGG 1 cut(s) 9
Bst2BI CACGAG 1 cut(s) 580
Bst2UI CCWGG 1 cut(s) 803
Bst6I CTCTTC 2 cut(s) 33, 253
BstACI GRCGYC 1 cut(s) 503
BstC8I GCNNGC 1 cut(s) 160
BstDEI CTNAG 1 cut(s) 378
BstDSI CCRYGG 1 cut(s) 9
BstF5I GGATG 2 cut(s) 5, 492
BstFNI CGCG 1 cut(s) 143
BstH2I RGCGCY 1 cut(s) 111
BstHHI GCGC 3 cut(s) 110, 145, 761
BstKTI GATC 6 cut(s) 174, 329, 688, 854, 880, 906
BstMAI GTCTC 1 cut(s) 268
BstMBI GATC 6 cut(s) 171, 326, 685, 851, 877, 903
BstMCI CGRYCG 3 cut(s) 854, 880, 906
BstMWI GCNNNNNNNGC 5 cut(s) 11, 164, 236, 245, 461
BstNI CCWGG 1 cut(s) 803
BstSCI CCNGG 4 cut(s) 102, 103, 178, 801
BstSFI CTRYAG 1 cut(s) 165
BstUI CGCG 1 cut(s) 143
BstV1I GCAGC 6 cut(s) 52, 97, 124, 179, 339, 427
BstV2I GAAGAC 1 cut(s) 498
BstX2I RGATCY 1 cut(s) 326
BstYI RGATCY 1 cut(s) 326
BsuRI GGCC 4 cut(s) 178, 830, 862, 914
BtgI CCRYGG 1 cut(s) 9
BtgZI GCGATG 1 cut(s) 693
BtrI CACGTC 1 cut(s) 217
BtsCI GGATG 2 cut(s) 5, 492
BtsI GCAGTG 1 cut(s) 162
BtsIMutI CAGTG 1 cut(s) 162
BveI ACCTGC 2 cut(s) 26, 898
Cac8I GCNNGC 1 cut(s) 160
CfoI GCGC 3 cut(s) 110, 145, 761
Cfr10I RCCGGY 1 cut(s) 145
Cfr13I GGNCC 5 cut(s) 177, 829, 861, 887, 913
Cfr9I CCCGGG 1 cut(s) 103
CseI GACGC 2 cut(s) 245, 254
Csp6I GTAC 1 cut(s) 281
CviAII CATG 2 cut(s) 10, 47
CviQI GTAC 1 cut(s) 281
DdeI CTNAG 1 cut(s) 378
DpnI GATC 6 cut(s) 173, 328, 687, 853, 879, 905
DpnII GATC 6 cut(s) 171, 326, 685, 851, 877, 903
DrdI GACNNNNNNGTC 1 cut(s) 730
DseDI GACNNNNNNGTC 1 cut(s) 730
Eam1104I CTCTTC 2 cut(s) 33, 253
EarI CTCTTC 2 cut(s) 33, 253
EciI GGCGGA 1 cut(s) 786
Eco130I CCWWGG 1 cut(s) 9
Eco47I GGWCC 1 cut(s) 887
Eco57I CTGAAG 1 cut(s) 612
Eco88I CYCGRG 2 cut(s) 103, 115
EcoO109I RGGNCCY 1 cut(s) 887
EcoRII CCWGG 1 cut(s) 801
EcoT14I CCWWGG 1 cut(s) 9
ErhI CCWWGG 1 cut(s) 9
FaeI CATG 2 cut(s) 13, 50
FaiI YATR 7 cut(s) 11, 48, 288, 335, 461, 541, 555
FalI AAGNNNNNCTT 2 cut(s) 54, 86
FaqI GGGAC 1 cut(s) 649
FatI CATG 2 cut(s) 9, 46
FblI GTMKAC 1 cut(s) 500
FokI GGATG 1 cut(s) 479
FspBI CTAG 2 cut(s) 132, 525
FspI TGCGCA 1 cut(s) 760
GlaI GCGC 3 cut(s) 109, 144, 760
GsaI CCCAGC 4 cut(s) 375, 835, 867, 919
HaeII RGCGCY 1 cut(s) 111
HaeIII GGCC 4 cut(s) 178, 830, 862, 914
HapII CCGG 4 cut(s) 104, 146, 180, 949
HgaI GACGC 2 cut(s) 245, 254
HhaI GCGC 3 cut(s) 110, 145, 761
Hin1I GRCGYC 1 cut(s) 503
Hin1II CATG 2 cut(s) 13, 50
Hin6I GCGC 3 cut(s) 108, 143, 759
HinP1I GCGC 3 cut(s) 108, 143, 759
HincII GTYRAC 1 cut(s) 501
HindII GTYRAC 1 cut(s) 501
HpaII CCGG 4 cut(s) 104, 146, 180, 949
HphI GGTGA 2 cut(s) 662, 695
Hpy166II GTNNAC 2 cut(s) 244, 501
Hpy188III TCNNGA 2 cut(s) 193, 960
Hpy8I GTNNAC 2 cut(s) 244, 501
Hpy99I CGWCG 6 cut(s) 194, 218, 502, 505, 824, 966
HpyAV CCTTC 4 cut(s) 219, 266, 355, 756
HpyCH4IV ACGT 3 cut(s) 216, 503, 638
HpyCH4V TGCA 3 cut(s) 68, 167, 443
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 164, 236, 245, 461
HpyF3I CTNAG 1 cut(s) 378
HpySE526I ACGT 3 cut(s) 216, 503, 638
Hsp92I GRCGYC 1 cut(s) 503
Hsp92II CATG 2 cut(s) 13, 50
HspAI GCGC 3 cut(s) 108, 143, 759
Kzo9I GATC 6 cut(s) 171, 326, 685, 851, 877, 903
LmnI GCTCC 2 cut(s) 872, 898
Lsp1109I GCAGC 6 cut(s) 52, 97, 124, 179, 339, 427
LweI GCATC 1 cut(s) 14
MaeI CTAG 2 cut(s) 132, 525
MaeII ACGT 3 cut(s) 216, 503, 638
MaeIII GTNAC 1 cut(s) 204
MalI GATC 6 cut(s) 173, 328, 687, 853, 879, 905
MboI GATC 6 cut(s) 171, 326, 685, 851, 877, 903
MboII GAAGA 9 cut(s) 20, 267, 270, 498, 507, 716, 729, 732, 741
MfeI CAATTG 2 cut(s) 564, 627
MflI RGATCY 1 cut(s) 326
MluCI AATT 6 cut(s) 97, 444, 564, 627, 644, 944
MlyI GAGTC 2 cut(s) 270, 840
MmeI TCCRAC 2 cut(s) 237, 566
MseI TTAA 1 cut(s) 473
MspA1I CMGCKG 2 cut(s) 352, 835
MspI CCGG 4 cut(s) 104, 146, 180, 949
MspR9I CCNGG 4 cut(s) 104, 105, 180, 803
MunI CAATTG 2 cut(s) 564, 627
MvaI CCWGG 1 cut(s) 803
MvnI CGCG 1 cut(s) 143
MwoI GCNNNNNNNGC 5 cut(s) 11, 164, 236, 245, 461
NciI CCSGG 3 cut(s) 104, 105, 180
NcoI CCATGG 1 cut(s) 9
NdeII GATC 6 cut(s) 171, 326, 685, 851, 877, 903
NlaIII CATG 2 cut(s) 13, 50
NlaIV GGNNCC 2 cut(s) 123, 187
NsbI TGCGCA 1 cut(s) 760
PaeR7I CTCGAG 1 cut(s) 115
PfeI GAWTC 8 cut(s) 653, 691, 699, 793, 840, 872, 898, 924
PflMI CCANNNNNTGG 1 cut(s) 632
Ple19I CGATCG 3 cut(s) 854, 880, 906
PleI GAGTC 2 cut(s) 269, 840
PpsI GAGTC 2 cut(s) 269, 840
PpuMI RGGWCCY 1 cut(s) 887
Psp5II RGGWCCY 1 cut(s) 887
Psp6I CCWGG 1 cut(s) 801
PspFI CCCAGC 4 cut(s) 371, 831, 863, 915
PspGI CCWGG 1 cut(s) 801
PspN4I GGNNCC 2 cut(s) 123, 187
PspPI GGNCC 5 cut(s) 177, 829, 861, 887, 913
PspPPI RGGWCCY 1 cut(s) 887
PspXI VCTCGAGB 1 cut(s) 115
PstI CTGCAG 1 cut(s) 169
PsuI RGATCY 1 cut(s) 326
PvuI CGATCG 3 cut(s) 854, 880, 906
RsaI GTAC 1 cut(s) 282
RsaNI GTAC 1 cut(s) 281
SalI GTCGAC 1 cut(s) 499
SaqAI TTAA 1 cut(s) 473
Sau3AI GATC 6 cut(s) 171, 326, 685, 851, 877, 903
Sau96I GGNCC 5 cut(s) 177, 829, 861, 887, 913
SchI GAGTC 2 cut(s) 270, 840
ScrFI CCNGG 4 cut(s) 104, 105, 180, 803
SfaNI GCATC 1 cut(s) 14
SfcI CTRYAG 1 cut(s) 165
Sfr274I CTCGAG 1 cut(s) 115
SgrDI CGTCGACG 1 cut(s) 499
SinI GGWCC 1 cut(s) 887
SlaI CTCGAG 1 cut(s) 115
SmaI CCCGGG 1 cut(s) 105
SmlI CTYRAG 2 cut(s) 115, 356
SmoI CTYRAG 2 cut(s) 115, 356
Sse9I AATT 6 cut(s) 97, 444, 564, 627, 644, 944
SsiI CCGC 6 cut(s) 141, 183, 350, 797, 806, 835
SspMI CTAG 2 cut(s) 132, 525
StyD4I CCNGG 4 cut(s) 102, 103, 178, 801
StyI CCWWGG 1 cut(s) 9
TaiI ACGT 3 cut(s) 219, 506, 641
TasI AATT 6 cut(s) 97, 444, 564, 627, 644, 944
TatI WGTACW 1 cut(s) 280
TauI GCSGC 4 cut(s) 143, 352, 809, 838
TfiI GAWTC 8 cut(s) 653, 691, 699, 793, 840, 872, 898, 924
Tru1I TTAA 1 cut(s) 473
Tru9I TTAA 1 cut(s) 473
TscAI CASTG 1 cut(s) 169
TseI GCWGC 6 cut(s) 65, 110, 137, 167, 352, 440
TspDTI ATGAA 4 cut(s) 285, 322, 387, 492
TspMI CCCGGG 1 cut(s) 103
TspRI CASTG 1 cut(s) 169
Van91I CCANNNNNTGG 1 cut(s) 632
VpaK11BI GGWCC 1 cut(s) 887
XapI RAATTY 1 cut(s) 644
XhoI CTCGAG 1 cut(s) 115
XmaI CCCGGG 1 cut(s) 103
XmiI GTMKAC 1 cut(s) 500
XspI CTAG 2 cut(s) 132, 525
ZraI GACGTC 1 cut(s) 504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.