Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0012252

Physical Location & Seq

Genomic Coordinates Forward (+)

11227889 .. 11230189

Gene Structure 2301 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1089700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2301 bp

ATGCTCATAAACCTCAGAAGAAGAATAACCATTCTTCCATTTCACACAAATCCTTCACCCTCCATTTCTCTGAATCTCATCTCAACTTCCACACAAATCCCACGCTCGGACTCTACAAATCCCCTATCAGAATCCCAACTCAAATCCTTAGTTCTAAGCCAATTCAGCCATGGCAAATTCACAAACCTCCTCCAAAACGTCGTCGCTTTGCCCGCTCTTCTCCTCACCGCCTGCCAAAACCTCACTTCCCCAAAAACCCAGAACGGCAACGGCCTCAGCCCCTCCCTTCTCGATTCGGTTTCGAAGCGGTTCTCAATCCACGAAATGGGTCGGCAGCTGTGCGAGAATCGGTTTGATGTCGGAGCTCGCAGCGTTGCAATGGCGGCCCAGAGAAACAGAGGTGAGTCTTTGGTATTGCCTAACTTGAAATTGAAGGTTTTGATTGAAGCTATTAGGATGGTATTGGGAATTGTTTATGATGAACGTTTTGTGACATTTTCTTATGGTGGTCGTGTTAATATGGGACGTCATACTGCCATTAGGTACCTTAAGAACTCTGTAGAAAACCCCAGTTGGTGGTTTAGTGTTGGATTTAACCGCGAAAAGTTTGATCAACGAAATGTGAATAAGTTGTGCTTATTTATGCAAGAGAAGATAGATGATGAGATTTTGATTGATGTTATAAAGAAGTTGTTTGAATGCGGCGCAGTGAGAATTGAGTTGGGTAGTTGTTGCCTTGGAAGAGGGTTTCCTCAGGAAAGCGGGTTGACTTCGATTTTGATGAATATCTACTTTAATGGGTTTGATAAAGAAATTCAAGAAATGCGTCTGAGGAAGAATCAAGAGCATCCGAAATTTGTTTCGAATGAACTTGTGTCGAAGCATGATGTGTTTTATAAGCCAGTGAAGATATATGCGGTTAGATACTTGGATGAGATATTAATAATAACCTCTGGATCTAAGATGTTGACAATGGATTTGAAGAACTGGGTTGTGAAGTACTTAGAGGGAACACTGGAATTGAAGGTGGATGGGCTAAAAACAGCTATTCATAGCGCAGTTTCTGAGAAGATTGATTTTATGGGGATGGAATTACAGGCAGTTCCGCCTTCGGTTTTGAATCCCCCCATGTCAGAAAAGGCCACCAGAGCACGAAAAAAGTACCTAAGACAGAAGGAAGTCAAAGCTCTGGAATTGAGAAATGCCAGAGAGAGGAACAGGAAGAAATTGGGATTGAAAATAATGAGTCATGTTTATAAGAAGTTGAAGAGAAGTAGTGGGTTTAAATCTGAACATCAAATTGAGAATGAAGTCAGGGAAATCTTCAGAACCTGGGGTGGCGAGACAGTGCAAGAGTTTCTTGGGTCGTTGGAGGAGCATTGGGAATGGTACCATAAATTATCAGCAGGTGATTTTCTCTCTTTGAGGCACATTAGAGATCAACTGCCTCAAGAGCTTGTTGATGCTTATGATAAGTTCCAAGGGCAAGTACACAAGCATTTGAACCCAGTCAAAGCTAGAAGGGCATTGGAGGAAGAAGAAAGGAGGGCAAAAGAAGAAGAGGAACAGAAATATGCTAAGGCCACAGTTGAGGATTTGGCAAAGCTCTGTGTGAAAGCCGATGCACCGATAGAACTTATTAGGAAGATGGTTAGGTTGATTGGGTTTACAAATCATATGGGTCGTCCCCAACCAATTACTTTACTAACTGCACTGGAAGATGCTGATATCATTAAGTGGTATGCGGGTATAGGGAGAAGATGGCTGGAATTTTACTGCTGCTGCCATAACTTCAAGATGGTCAAAACTGTTGTGACTTATCACATGAGGTTCTCTTGCATATTGACTCTAGCGGAAAAGCACGAATCCACCAAACAGGAAGCCATGAAACATTATACTAAAGATTTGAAAGTCTTTGATTTAGATGGAAATGAAGAAGTACACTTCCCCACAGAAAGGGAAGTGAAAATGATGGGAGATAAAAATCTCTCTGATCCAAAACCTGTGGATGGGGCGCTATCTTTGGCTTTGATTAGATTAGCTTCTGATGAGCCTCCGTATTCATGTGTCGCTCATTTCTGCAACAGAACTGATACCATTGTTTATCGGGTACGACTACTGCAAAATCACTTGAATGTGAACCCCATGGATGAAAAGAAATGGGTCCCTGGTATGGGTGCAATTAACGAAAGCCTCAACTTGAAATGTTTTCCGGTTTGTCCTGATCATACACATGATTTATACATGGGGAGAATCACCTTTCAGGACATTGATTGTACTTCATTTGTGGAGGTAGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000373 GO:0000375 GO:0000377 GO:0000959 GO:0000963 GO:0003674 GO:0003824 GO:0003964 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006259 GO:0006278 GO:0006310 GO:0006314 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0071897 GO:0090304 GO:0090615 GO:0140053 GO:0140097 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Intron_maturas2 RVT_1

Protein Analysis

767

Amino Acids

88.13

Weight (kDa)

8.95

Isoelectric Point (pI)

46.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Intron_maturas2	PF01348	541 - 646	5.4e-16	Type II intron maturase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0012252)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G04050 AT5G04050
fragaria_vesca	FvH4_6g25070
malus_domestica	MD12G1089700.v1.1
prunus_persica	Prupe.7G047200_v2.0.a1 Prupe.7G047200_v2.0.a1 Prupe.7G047200_v2.0.a1
pyrus_communis	pycom12g07560
rosa_chinensis	RchiOBHm_Chr2g0124831
rosa_laevigata	RLG00000018826
rosa_multiflora	Rmu_co8339159.1_g000001
rosa_roxburghii	Rroxscaffold_2G00119620
rosa_rugosa	Rorug02G0253000
rosa_samantha	Rh2AG068200 Rh2AG312500 Rh2BG320700 Rh2CG300700 Rh2DG338000
rosa_wichuraiana	Rw2G025240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	683, 897, 1257
AarI	CACCTGC	1 cut(s)	1397
AatII	GACGTC	1 cut(s)	529
Acc36I	ACCTGC	1 cut(s)	1397
Acc65I	GGTACC	2 cut(s)	543, 1389
AccB1I	GGYRCC	2 cut(s)	543, 1389
AccB7I	CCANNNNNTGG	2 cut(s)	325, 576
AccBSI	CCGCTC	1 cut(s)	215
AccI	GTMKAC	1 cut(s)	2295
AccII	CGCG	1 cut(s)	600
AclI	AACGTT	1 cut(s)	484
AclWI	GGATC	2 cut(s)	964, 1988
AcsI	RAATTY	4 cut(s)	176, 813, 854, 1769
AcuI	CTGAAG	1 cut(s)	1309
AcyI	GRCGYC	1 cut(s)	526
AfaI	GTAC	8 cut(s)	545, 1001, 1163, 1391, 1491, 1941, 2112, 2278
AfiI	CCNNNNNNNGG	8 cut(s)	106, 325, 576, 1212, 1956, 2009, 2173, 2174
AflII	CTTAAG	1 cut(s)	548
AjnI	CCWGG	2 cut(s)	1331, 2167
AluBI	AGCT	9 cut(s)	337, 365, 449, 1046, 1187, 1456, 1517, 1606, 2042
AluI	AGCT	9 cut(s)	337, 365, 449, 1046, 1187, 1456, 1517, 1606, 2042
Alw21I	GWGCWC	2 cut(s)	367, 1153
Alw26I	GTCTC	1 cut(s)	1337
AlwI	GGATC	2 cut(s)	964, 1988
AlwNI	CAGNNNCTG	2 cut(s)	1064, 1332
AoxI	GGCC	4 cut(s)	271, 384, 1140, 1581
ApeKI	GCWGC	4 cut(s)	334, 369, 1779, 1782
ApoI	RAATTY	4 cut(s)	176, 813, 854, 1769
ArsI	GACNNNNNNTTYG	4 cut(s)	760, 792, 961, 993
AseI	ATTAAT	1 cut(s)	941
Asp700I	GAANNNNTTC	1 cut(s)	308
Asp718I	GGTACC	2 cut(s)	543, 1389
AspLEI	GCGC	3 cut(s)	707, 1058, 2017
AspS9I	GGNCC	2 cut(s)	385, 2164
AsuHPI	GGTGA	5 cut(s)	48, 217, 413, 1421, 2248
AsuII	TTCGAA	2 cut(s)	302, 863
AvaII	GGWCC	1 cut(s)	2164
AxyI	CCTNAGG	1 cut(s)	753
BaeI	ACNNNNGTAYC	2 cut(s)	2085, 2118
BanI	GGYRCC	2 cut(s)	543, 1389
BanII	GRGCYC	1 cut(s)	367
Bbv12I	GWGCWC	2 cut(s)	367, 1153
BbvCI	CCTCAGC	1 cut(s)	275
BbvI	GCAGC	4 cut(s)	346, 381, 1766, 1769
BccI	CCATC	9 cut(s)	451, 1025, 1081, 1642, 1755, 1792, 1919, 1966, 2003
BceAI	ACGGC	2 cut(s)	280, 286
BciT130I	CCWGG	2 cut(s)	1333, 2169
BclI	TGATCA	2 cut(s)	610, 2224
BcoDI	GTCTC	1 cut(s)	1337
BfaI	CTAG	2 cut(s)	1518, 1850
BfmI	CTRYAG	1 cut(s)	558
BfoI	RGCGCY	1 cut(s)	2018
BfrI	CTTAAG	1 cut(s)	548
BfuAI	ACCTGC	1 cut(s)	1397
BisI	GCNGC	6 cut(s)	335, 370, 384, 703, 1780, 1783
BlsI	GCNGC	6 cut(s)	336, 371, 385, 704, 1781, 1784
BmcAI	AGTACT	1 cut(s)	1001
Bme1390I	CCNGG	2 cut(s)	1333, 2169
Bme18I	GGWCC	1 cut(s)	2164
BmgT120I	GGNCC	2 cut(s)	385, 2164
BmiI	GGNNCC	4 cut(s)	545, 1391, 2165, 2166
BmrFI	CCNGG	2 cut(s)	1333, 2169
BmrI	ACTGGG	3 cut(s)	564, 997, 1502
BmsI	GCATC	4 cut(s)	856, 1453, 1612, 1711
BmuI	ACTGGG	3 cut(s)	564, 997, 1502
Bpu10I	CCTNAGC	2 cut(s)	275, 1578
Bpu14I	TTCGAA	2 cut(s)	302, 863
BpuEI	CTTGAG	1 cut(s)	1434
BsaBI	GATNNNNATC	2 cut(s)	785, 1983
BsaHI	GRCGYC	1 cut(s)	526
BsaJI	CCNNGG	6 cut(s)	169, 736, 1332, 1480, 2145, 2167
BsaWI	WCCGGW	1 cut(s)	2212
Bsc4I	CCNNNNNNNGG	8 cut(s)	106, 325, 576, 1212, 1956, 2009, 2173, 2174
Bse1I	ACTGG	6 cut(s)	570, 902, 992, 1020, 1508, 1719
Bse21I	CCTNAGG	1 cut(s)	753
Bse3DI	GCAATG	1 cut(s)	384
Bse8I	GATNNNNATC	2 cut(s)	785, 1983
BseBI	CCWGG	2 cut(s)	1333, 2169
BseDI	CCNNGG	6 cut(s)	169, 736, 1332, 1480, 2145, 2167
BseGI	GGATG	7 cut(s)	462, 847, 937, 1036, 1092, 2014, 2155
BseJI	GATNNNNATC	2 cut(s)	785, 1983
BseLI	CCNNNNNNNGG	8 cut(s)	106, 325, 576, 1212, 1956, 2009, 2173, 2174
BseMI	GCAATG	1 cut(s)	384
BseMII	CTCAG	5 cut(s)	28, 289, 767, 821, 1056
BseNI	ACTGG	6 cut(s)	570, 902, 992, 1020, 1508, 1719
BseRI	GAGGAG	3 cut(s)	179, 212, 1388
BseXI	GCAGC	4 cut(s)	346, 381, 1766, 1769
BsgI	GTGCAG	1 cut(s)	1695
Bsh1236I	CGCG	1 cut(s)	600
BshFI	GGCC	4 cut(s)	273, 386, 1142, 1583
BshNI	GGYRCC	2 cut(s)	543, 1389
BsiHKAI	GWGCWC	2 cut(s)	367, 1153
BsiSI	CCGG	1 cut(s)	2213
BslFI	GGGAC	3 cut(s)	537, 1671, 2150
BslI	CCNNNNNNNGG	8 cut(s)	106, 325, 576, 1212, 1956, 2009, 2173, 2174
BsmAI	GTCTC	1 cut(s)	1337
BsmFI	GGGAC	3 cut(s)	537, 1671, 2150
BsmI	GAATGC	1 cut(s)	704
BsnI	GGCC	4 cut(s)	273, 386, 1142, 1583
Bsp119I	TTCGAA	2 cut(s)	302, 863
Bsp1286I	GDGCHC	2 cut(s)	367, 1153
Bsp143I	GATC	5 cut(s)	610, 956, 1438, 1993, 2224
Bsp19I	CCATGG	2 cut(s)	169, 2145
BspANI	GGCC	4 cut(s)	273, 386, 1142, 1583
BspCNI	CTCAG	5 cut(s)	27, 288, 766, 822, 1057
BspFNI	CGCG	1 cut(s)	600
BspLI	GGNNCC	4 cut(s)	545, 1391, 2165, 2166
BspMI	ACCTGC	1 cut(s)	1397
BspPI	GGATC	2 cut(s)	964, 1988
BspQI	GCTCTTC	1 cut(s)	222
BspT104I	TTCGAA	2 cut(s)	302, 863
BspT107I	GGYRCC	2 cut(s)	543, 1389
BspTI	CTTAAG	1 cut(s)	548
BsrBI	CCGCTC	1 cut(s)	215
BsrDI	GCAATG	1 cut(s)	384
BsrI	ACTGG	6 cut(s)	570, 902, 992, 1020, 1508, 1719
BssECI	CCNNGG	6 cut(s)	169, 736, 1332, 1480, 2145, 2167
BssMI	GATC	5 cut(s)	610, 956, 1438, 1993, 2224
BssNI	GRCGYC	1 cut(s)	526
BssT1I	CCWWGG	4 cut(s)	169, 736, 1480, 2145
Bst2UI	CCWGG	2 cut(s)	1333, 2169
Bst4CI	ACNGT	3 cut(s)	1348, 1588, 1810
Bst6I	CTCTTC	4 cut(s)	222, 736, 1262, 1554
BstACI	GRCGYC	1 cut(s)	526
BstAFI	CTTAAG	1 cut(s)	548
BstBI	TTCGAA	2 cut(s)	302, 863
BstC8I	GCNNGC	3 cut(s)	213, 232, 367
BstDSI	CCRYGG	2 cut(s)	169, 2145
BstF5I	GGATG	7 cut(s)	462, 847, 937, 1036, 1092, 2014, 2155
BstFNI	CGCG	1 cut(s)	600
BstH2I	RGCGCY	1 cut(s)	2018
BstHHI	GCGC	3 cut(s)	707, 1058, 2017
BstKTI	GATC	5 cut(s)	613, 959, 1441, 1996, 2227
BstMAI	GTCTC	1 cut(s)	1337
BstMBI	GATC	5 cut(s)	610, 956, 1438, 1993, 2224
BstMWI	GCNNNNNNNGC	6 cut(s)	165, 212, 383, 1148, 1453, 1523
BstNI	CCWGG	2 cut(s)	1333, 2169
BstSCI	CCNGG	2 cut(s)	1331, 2167
BstSFI	CTRYAG	1 cut(s)	558
BstUI	CGCG	1 cut(s)	600
BstV1I	GCAGC	4 cut(s)	346, 381, 1766, 1769
BstX2I	RGATCY	1 cut(s)	956
BstYI	RGATCY	1 cut(s)	956
Bsu36I	CCTNAGG	1 cut(s)	753
BsuRI	GGCC	4 cut(s)	273, 386, 1142, 1583
BtgI	CCRYGG	2 cut(s)	169, 2145
BtsCI	GGATG	7 cut(s)	462, 847, 937, 1036, 1092, 2014, 2155
BtsI	GCAGTG	1 cut(s)	714
BtsIMutI	CAGTG	5 cut(s)	714, 909, 1013, 1353, 1712
BveI	ACCTGC	1 cut(s)	1397
Cac8I	GCNNGC	3 cut(s)	213, 232, 367
CaiI	CAGNNNCTG	2 cut(s)	1064, 1332
CfoI	GCGC	3 cut(s)	707, 1058, 2017
Cfr13I	GGNCC	2 cut(s)	385, 2164
CseI	GACGC	1 cut(s)	815
Csp6I	GTAC	8 cut(s)	544, 1000, 1162, 1390, 1490, 1940, 2111, 2277
CspCI	CAANNNNNGTGG	2 cut(s)	1986, 2021
CviQI	GTAC	8 cut(s)	544, 1000, 1162, 1390, 1490, 1940, 2111, 2277
DpnI	GATC	5 cut(s)	612, 958, 1440, 1995, 2226
DpnII	GATC	5 cut(s)	610, 956, 1438, 1993, 2224
DraI	TTTAAA	1 cut(s)	1285
Eam1104I	CTCTTC	4 cut(s)	222, 736, 1262, 1554
EarI	CTCTTC	4 cut(s)	222, 736, 1262, 1554
EciI	GGCGGA	1 cut(s)	1095
Ecl136II	GAGCTC	1 cut(s)	365
Eco130I	CCWWGG	4 cut(s)	169, 736, 1480, 2145
Eco24I	GRGCYC	1 cut(s)	367
Eco32I	GATATC	1 cut(s)	1729
Eco47I	GGWCC	1 cut(s)	2164
Eco53kI	GAGCTC	1 cut(s)	365
Eco57I	CTGAAG	1 cut(s)	1309
Eco81I	CCTNAGG	1 cut(s)	753
EcoICRI	GAGCTC	1 cut(s)	365
EcoO109I	RGGNCCY	1 cut(s)	2164
EcoRII	CCWGG	2 cut(s)	1331, 2167
EcoRV	GATATC	1 cut(s)	1729
EcoT14I	CCWWGG	4 cut(s)	169, 736, 1480, 2145
EcoT38I	GRGCYC	1 cut(s)	367
ErhI	CCWWGG	4 cut(s)	169, 736, 1480, 2145
FalI	AAGNNNNNCTT	4 cut(s)	620, 652, 1344, 1376
FaqI	GGGAC	3 cut(s)	537, 1671, 2150
FauI	CCCGC	3 cut(s)	220, 755, 1738
FauNDI	CATATG	1 cut(s)	1677
FbaI	TGATCA	2 cut(s)	610, 2224
FblI	GTMKAC	1 cut(s)	2295
Fnu4HI	GCNGC	6 cut(s)	335, 370, 384, 703, 1780, 1783
FokI	GGATG	7 cut(s)	469, 834, 944, 1043, 1099, 2021, 2162
FriOI	GRGCYC	1 cut(s)	367
Fsp4HI	GCNGC	6 cut(s)	335, 370, 384, 703, 1780, 1783
FspBI	CTAG	2 cut(s)	1518, 1850
GlaI	GCGC	3 cut(s)	706, 1057, 2016
GluI	GCNGC	6 cut(s)	335, 370, 384, 703, 1780, 1783
HaeII	RGCGCY	1 cut(s)	2018
HaeIII	GGCC	4 cut(s)	273, 386, 1142, 1583
HapII	CCGG	1 cut(s)	2213
HgaI	GACGC	1 cut(s)	815
HhaI	GCGC	3 cut(s)	707, 1058, 2017
Hin1I	GRCGYC	1 cut(s)	526
Hin6I	GCGC	3 cut(s)	705, 1056, 2015
HinP1I	GCGC	3 cut(s)	705, 1056, 2015
HincII	GTYRAC	2 cut(s)	768, 969
HindII	GTYRAC	2 cut(s)	768, 969
HpaII	CCGG	1 cut(s)	2213
HphI	GGTGA	5 cut(s)	48, 217, 413, 1421, 2248
Hpy166II	GTNNAC	7 cut(s)	768, 969, 1492, 1668, 1942, 2140, 2296
Hpy8I	GTNNAC	7 cut(s)	768, 969, 1492, 1668, 1942, 2140, 2296
Hpy99I	CGWCG	2 cut(s)	203, 206
HpyAV	CCTTC	7 cut(s)	63, 296, 427, 1018, 1119, 1168, 1515
HpyCH4III	ACNGT	3 cut(s)	1348, 1588, 1810
HpyCH4IV	ACGT	3 cut(s)	198, 484, 526
HpyCH4V	TGCA	9 cut(s)	377, 646, 1351, 1625, 1712, 1839, 2082, 2122, 2180
HpyF10VI	GCNNNNNNNGC	6 cut(s)	165, 212, 383, 1148, 1453, 1523
HpySE526I	ACGT	3 cut(s)	198, 484, 526
Hsp92I	GRCGYC	1 cut(s)	526
HspAI	GCGC	3 cut(s)	705, 1056, 2015
KflI	GGGWCCC	1 cut(s)	2164
KpnI	GGTACC	2 cut(s)	547, 1393
Ksp22I	TGATCA	2 cut(s)	610, 2224
Kzo9I	GATC	5 cut(s)	610, 956, 1438, 1993, 2224
LguI	GCTCTTC	1 cut(s)	222
LmnI	GCTCC	2 cut(s)	362, 1375
Lsp1109I	GCAGC	4 cut(s)	346, 381, 1766, 1769
LweI	GCATC	4 cut(s)	856, 1453, 1612, 1711
MaeI	CTAG	2 cut(s)	1518, 1850
MaeII	ACGT	3 cut(s)	198, 484, 526
MaeIII	GTNAC	2 cut(s)	490, 1813
MalI	GATC	5 cut(s)	612, 958, 1440, 1995, 2226
MbiI	CCGCTC	1 cut(s)	215
MboI	GATC	5 cut(s)	610, 956, 1438, 1993, 2224
MflI	RGATCY	1 cut(s)	956
MhlI	GDGCHC	2 cut(s)	367, 1153
MlyI	GAGTC	4 cut(s)	104, 413, 1255, 1840
MmeI	TCCRAC	3 cut(s)	340, 568, 1350
MroXI	GAANNNNTTC	1 cut(s)	308
MseI	TTAA	8 cut(s)	516, 549, 594, 795, 941, 1284, 1734, 2184
MslI	CAYNNNNRTG	2 cut(s)	2133, 2232
MspA1I	CMGCKG	1 cut(s)	337
MspCI	CTTAAG	1 cut(s)	548
MspI	CCGG	1 cut(s)	2213
MspR9I	CCNGG	2 cut(s)	1333, 2169
Mva1269I	GAATGC	1 cut(s)	704
MvaI	CCWGG	2 cut(s)	1333, 2169
MvnI	CGCG	1 cut(s)	600
MwoI	GCNNNNNNNGC	6 cut(s)	165, 212, 383, 1148, 1453, 1523
NcoI	CCATGG	2 cut(s)	169, 2145
NdeI	CATATG	1 cut(s)	1677
NdeII	GATC	5 cut(s)	610, 956, 1438, 1993, 2224
NlaIV	GGNNCC	4 cut(s)	545, 1391, 2165, 2166
NmuCI	GTSAC	2 cut(s)	490, 1813
NspV	TTCGAA	2 cut(s)	302, 863
PaqCI	CACCTGC	1 cut(s)	1397
PciSI	GCTCTTC	1 cut(s)	222
PctI	GAATGC	1 cut(s)	704
PdmI	GAANNNNTTC	1 cut(s)	308
PfeI	GAWTC	8 cut(s)	73, 131, 293, 346, 838, 1120, 1865, 2253
PflMI	CCANNNNNTGG	2 cut(s)	325, 576
PkrI	GCNGC	6 cut(s)	336, 371, 385, 704, 1781, 1784
PleI	GAGTC	4 cut(s)	104, 412, 1254, 1840
PpsI	GAGTC	4 cut(s)	104, 412, 1254, 1840
PpuMI	RGGWCCY	1 cut(s)	2164
PshBI	ATTAAT	1 cut(s)	941
PsiI	TTATAA	3 cut(s)	683, 897, 1257
Psp124BI	GAGCTC	1 cut(s)	367
Psp1406I	AACGTT	1 cut(s)	484
Psp5II	RGGWCCY	1 cut(s)	2164
Psp6I	CCWGG	2 cut(s)	1331, 2167
PspGI	CCWGG	2 cut(s)	1331, 2167
PspN4I	GGNNCC	4 cut(s)	545, 1391, 2165, 2166
PspPI	GGNCC	2 cut(s)	385, 2164
PspPPI	RGGWCCY	1 cut(s)	2164
PstNI	CAGNNNCTG	2 cut(s)	1064, 1332
PsuI	RGATCY	1 cut(s)	956
PvuII	CAGCTG	1 cut(s)	337
RsaI	GTAC	8 cut(s)	545, 1001, 1163, 1391, 1491, 1941, 2112, 2278
RsaNI	GTAC	8 cut(s)	544, 1000, 1162, 1390, 1490, 1940, 2111, 2277
RseI	CAYNNNNRTG	2 cut(s)	2133, 2232
SacI	GAGCTC	1 cut(s)	367
SapI	GCTCTTC	1 cut(s)	222
SaqAI	TTAA	8 cut(s)	516, 549, 594, 795, 941, 1284, 1734, 2184
SatI	GCNGC	6 cut(s)	335, 370, 384, 703, 1780, 1783
Sau3AI	GATC	5 cut(s)	610, 956, 1438, 1993, 2224
Sau96I	GGNCC	2 cut(s)	385, 2164
ScaI	AGTACT	1 cut(s)	1001
SchI	GAGTC	4 cut(s)	104, 413, 1255, 1840
ScrFI	CCNGG	2 cut(s)	1333, 2169
SduI	GDGCHC	2 cut(s)	367, 1153
SfaNI	GCATC	4 cut(s)	856, 1453, 1612, 1711
SfcI	CTRYAG	1 cut(s)	558
SfuI	TTCGAA	2 cut(s)	302, 863
SinI	GGWCC	1 cut(s)	2164
SmiMI	CAYNNNNRTG	2 cut(s)	2133, 2232
SmlI	CTYRAG	2 cut(s)	548, 1449
SmoI	CTYRAG	2 cut(s)	548, 1449
SspMI	CTAG	2 cut(s)	1518, 1850
SstI	GAGCTC	1 cut(s)	367
StyD4I	CCNGG	2 cut(s)	1331, 2167
StyI	CCWWGG	4 cut(s)	169, 736, 1480, 2145
TaaI	ACNGT	3 cut(s)	1348, 1588, 1810
TaiI	ACGT	3 cut(s)	201, 487, 529
TaqI	TCGA	5 cut(s)	291, 302, 773, 863, 878
TatI	WGTACW	4 cut(s)	999, 1489, 1939, 2276
TauI	GCSGC	2 cut(s)	386, 705
TfiI	GAWTC	8 cut(s)	73, 131, 293, 346, 838, 1120, 1865, 2253
Tru1I	TTAA	8 cut(s)	516, 549, 594, 795, 941, 1284, 1734, 2184
Tru9I	TTAA	8 cut(s)	516, 549, 594, 795, 941, 1284, 1734, 2184
TscAI	CASTG	5 cut(s)	714, 909, 1020, 1353, 1719
TseFI	GTSAC	2 cut(s)	490, 1813
TseI	GCWGC	4 cut(s)	334, 369, 1779, 1782
Tsp45I	GTSAC	2 cut(s)	490, 1813
TspGWI	ACGGA	1 cut(s)	2046
TspRI	CASTG	5 cut(s)	714, 909, 1020, 1353, 1719
Van91I	CCANNNNNTGG	2 cut(s)	325, 576
Vha464I	CTTAAG	1 cut(s)	548
VpaK11BI	GGWCC	1 cut(s)	2164
VspI	ATTAAT	1 cut(s)	941
XapI	RAATTY	4 cut(s)	176, 813, 854, 1769
XcmI	CCANNNNNNNNNTGG	1 cut(s)	167
XmiI	GTMKAC	1 cut(s)	2295
XmnI	GAANNNNTTC	1 cut(s)	308
XspI	CTAG	2 cut(s)	1518, 1850
ZraI	GACGTC	1 cut(s)	527
ZrmI	AGTACT	1 cut(s)	1001

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.