Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0012252

Physical Location & Seq

Genomic Coordinates Forward (+)

38370540 .. 38372516

Gene Structure 1977 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG320700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1977 bp

ATGGGTCGCGAGCTCTGCGAGAATCGGTTCGATGTGGCAGCTTCTAGTGTCACAATGGCGGCCCCAAGAAACGGAGGTGAGTCTCTGGTCTTACCCAGTTTGAAACTCAAAGTTTTGATTGAAGCTATTAGGATTGTATTGGGGGTTGTTTATGATGAACGCTTTGTGACGTTTTCTTACGGCGGTCGTGTTAATATGGGGAGGCACACGGCCATTAGGTACCTTAAGAACTCTGTTGCAAATCCCAGTTGGTGGTTTAGCGTTAGCTTTAATGGGGGTAAGTTTGAACAACGACATGTAAATAAGCTATGCTTGTTTATGCACGAGAAAATAGAGGATGAGGTTTTGACTAATATCATCAAGACATTGTTTGAATGTGGCGCGGTGAGAATTGAATTGGGTAGTTGTTGCTTTGGAAGAGGGTTTCCTCAGGAGAGTGGGTTGAGTTCGATTTTAATGAACATTTACTTTAATGGTTTCGACAAAGAAATTCAAGAGATGCGTCTGAAGAAGAATCAGGAGCATCCCAAATTCGAGTCGAATGAGCTTGTTTCAGAGGATGGTGTTTTCTATAAGCCTGTCAAGATATATGCGGTTAGGTACTTGGATGAGATATTAGTGATGACGTCTGGGTCCAAAATGTTGACTATGGATTTGAAGAACTGGGTTGTCAAGTACTTAGAAGGGAGTTTGGAATTGATGGTGGATAAGATTAAAACATCTATTCATAGTGCAGTGTCTGAGAAGATTGATTTCATGGGCATGGAATTACAGGCAGTTCCACCTTCGGTGTTGCACCCCCCTATGTCTGAAAAAGCTATTAGAGCACGAAAAAAGTACCTTAGACAGAAGGAAGTCAGAGCTATAGAATTGAAAAATGCTAGAGAGAGGAACAGAAAAAAATTGGGAATGAAAATAATGAGCCATGTTTTTAAAAAGTTGAAGAGCAGTGATGGATTGAAATCTGAGTATCAAATTGAGAATCAAGTCAGAGAAATCTTTAGAACCTGGGCTGATGAAGTGGTGCAAGAGTTCCTTGAGTCATTGGATGAGCGTTGGGATTGGTATCGAAAGCTATCAGCAGGTAATTTTCTGTCTTTAAGACATATTAGACAACAATTGCCCGAAGAGCTTGTAGATGCTTATGATAAGTTCCAAGAGCAAGTAGACAAGCATTTGAACCCAGTCAGAGATAGAAAAGCAAGAGAGGAAGAAGAAAGGAGGGTAACAGAAGAAGCGGAGCAGAAATATGCCAAGAGAACAGTTGAGGATTTGACAAAGCTCTGTGTGAAAGCTGATGCGCCTATAGAAGTTTTGAGGAAGATGGTCAAGTTAATTGGGTTTACAAATCATATGGGTCGTCCCAGGCCAATCACTTTACTAACTGCTCTTGAAGATGCTGATATCATCAAGTGGTATGCAGGTGTAGGGAGAAGATTGCTTGATTTTTACTGCTGCTGCCATAACTTCAAGATGGTCAAAACTATTGTAACTTATCACTTGAGGTTCTCCTGCATCTTGACTCTTGCTGAAAAGCATGAATCTACCAAAAGTGAAGCCATGAAACATTATACTAAAGATTTGAAAGTCTTTGATATAAATGGAAATCAAGAAGTATACTTTCCCACAGAAAGAGAGGTAAAGATGATGGGAGATAAAAATCTTTCTGATCCAATACCTGTGGATGGGGCATTTTCTTTGGCTCTGATTAGATTAGCTTCTGATGAGCCTCCATATTCTTGTGTTGCTCATTTCTGTGACAGAACTGATACTATTGTTTATCGGGTACGATTATTGCAAAGTCGATTGAATCTGACCCCCGTGGATGATAAGAAATGGGTGCCGGGGATGGGTGCAATTAATGAGAGCCTCCATCTGAAATGCTTCCCTCTTTGTCCTGATCACATACATGATCTATACATGGGGAGCATTACCTTTCAAGATATTGATTGTACATCATTTGTGGATGTAGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000373 GO:0000375 GO:0000377 GO:0000959 GO:0000963 GO:0003674 GO:0003824 GO:0003964 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006259 GO:0006278 GO:0006310 GO:0006314 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0071897 GO:0090304 GO:0090615 GO:0140053 GO:0140097 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Intron_maturas2 RVT_1

Protein Analysis

658

Amino Acids

76.11

Weight (kDa)

8.32

Isoelectric Point (pI)

44.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Intron_maturas2	PF01348	433 - 557	7.9e-17	Type II intron maturase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Orthologous Genes (Group: OG0012252)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G04050 AT5G04050
fragaria_vesca	FvH4_6g25070
malus_domestica	MD12G1089700.v1.1
prunus_persica	Prupe.7G047200_v2.0.a1 Prupe.7G047200_v2.0.a1 Prupe.7G047200_v2.0.a1
pyrus_communis	pycom12g07560
rosa_chinensis	RchiOBHm_Chr2g0124831
rosa_laevigata	RLG00000018826
rosa_multiflora	Rmu_co8339159.1_g000001
rosa_roxburghii	Rroxscaffold_2G00119620
rosa_rugosa	Rorug02G0253000
rosa_samantha	Rh2AG068200 Rh2AG312500 Rh2BG320700 Rh2CG300700 Rh2DG338000
rosa_wichuraiana	Rw2G025240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1412
AasI	GACNNNNNNGTC	1 cut(s)	631
AatII	GACGTC	1 cut(s)	629
Acc36I	ACCTGC	2 cut(s)	1073, 1412
Acc65I	GGTACC	1 cut(s)	219
AccB1I	GGYRCC	2 cut(s)	219, 1840
AccB7I	CCANNNNNTGG	1 cut(s)	252
AccI	GTMKAC	3 cut(s)	1167, 1617, 1971
AccII	CGCG	2 cut(s)	9, 383
AciI	CCGC	5 cut(s)	59, 183, 383, 593, 1238
AclWI	GGATC	1 cut(s)	1664
AcoI	YGGCCR	1 cut(s)	210
AcsI	RAATTY	2 cut(s)	489, 530
AcuI	CTGAAG	1 cut(s)	527
AcyI	GRCGYC	1 cut(s)	626
AfaI	GTAC	6 cut(s)	221, 602, 677, 839, 1788, 1954
AfiI	CCNNNNNNNGG	4 cut(s)	71, 252, 1685, 1850
AflII	CTTAAG	1 cut(s)	224
AflIII	ACRYGT	1 cut(s)	295
AjnI	CCWGG	2 cut(s)	1007, 1364
Alw21I	GWGCWC	2 cut(s)	15, 829
Alw26I	GTCTC	1 cut(s)	87
AlwI	GGATC	1 cut(s)	1664
AlwNI	CAGNNNCTG	1 cut(s)	740
AoxI	GGCC	3 cut(s)	60, 210, 1367
ApeKI	GCWGC	3 cut(s)	38, 1455, 1458
ApoI	RAATTY	2 cut(s)	489, 530
ArsI	GACNNNNNNTTYG	2 cut(s)	637, 669
AseI	ATTAAT	1 cut(s)	1860
Asp700I	GAANNNNTTC	2 cut(s)	26, 1883
Asp718I	GGTACC	1 cut(s)	219
AspLEI	GCGC	2 cut(s)	383, 1303
AspS9I	GGNCC	2 cut(s)	61, 633
AsuC2I	CCSGG	1 cut(s)	1845
AsuHPI	GGTGA	2 cut(s)	89, 397
AvaII	GGWCC	1 cut(s)	633
AxyI	CCTNAGG	1 cut(s)	429
BaeI	ACNNNNGTAYC	2 cut(s)	1761, 1794
BanI	GGYRCC	2 cut(s)	219, 1840
BanII	GRGCYC	1 cut(s)	15
BauI	CACGAG	1 cut(s)	323
Bbv12I	GWGCWC	2 cut(s)	15, 829
BbvI	GCAGC	3 cut(s)	50, 1442, 1445
BccI	CCATC	9 cut(s)	554, 694, 947, 1318, 1468, 1642, 1679, 1843, 1881
BceAI	ACGGC	2 cut(s)	196, 225
BciT130I	CCWGG	2 cut(s)	1009, 1366
BclI	TGATCA	1 cut(s)	1900
BcnI	CCSGG	1 cut(s)	1845
BcoDI	GTCTC	1 cut(s)	87
BfaI	CTAG	2 cut(s)	45, 882
BfmI	CTRYAG	2 cut(s)	864, 1305
BfrI	CTTAAG	1 cut(s)	224
BfuAI	ACCTGC	2 cut(s)	1073, 1412
BisI	GCNGC	4 cut(s)	39, 60, 1456, 1459
BlsI	GCNGC	4 cut(s)	40, 61, 1457, 1460
BmcAI	AGTACT	1 cut(s)	677
Bme1390I	CCNGG	3 cut(s)	1009, 1366, 1845
Bme18I	GGWCC	1 cut(s)	633
BmgT120I	GGNCC	2 cut(s)	61, 633
BmiI	GGNNCC	4 cut(s)	63, 221, 634, 1842
BmrFI	CCNGG	3 cut(s)	1009, 1366, 1845
BmrI	ACTGGG	4 cut(s)	90, 240, 673, 1178
BmsI	GCATC	6 cut(s)	489, 532, 1129, 1288, 1387, 1524
BmuI	ACTGGG	4 cut(s)	90, 240, 673, 1178
BpuEI	CTTGAG	2 cut(s)	1058, 1522
BpuMI	CCSGG	1 cut(s)	1845
BsaBI	GATNNNNATC	3 cut(s)	961, 1065, 1659
BsaHI	GRCGYC	1 cut(s)	626
BsaJI	CCNNGG	4 cut(s)	1008, 1364, 1821, 1844
BsaXI	ACNNNNNCTCC	2 cut(s)	425, 455
Bsc4I	CCNNNNNNNGG	4 cut(s)	71, 252, 1685, 1850
Bse1I	ACTGG	4 cut(s)	96, 246, 668, 1184
Bse21I	CCTNAGG	1 cut(s)	429
Bse8I	GATNNNNATC	3 cut(s)	961, 1065, 1659
BseBI	CCWGG	2 cut(s)	1009, 1366
BseDI	CCNNGG	4 cut(s)	1008, 1364, 1821, 1844
BseGI	GGATG	9 cut(s)	343, 523, 565, 613, 1054, 1690, 1831, 1854, 1972
BseJI	GATNNNNATC	3 cut(s)	961, 1065, 1659
BseLI	CCNNNNNNNGG	4 cut(s)	71, 252, 1685, 1850
BseMII	CTCAG	3 cut(s)	443, 732, 957
BseNI	ACTGG	4 cut(s)	96, 246, 668, 1184
BseXI	GCAGC	3 cut(s)	50, 1442, 1445
BsgI	GTGCAG	1 cut(s)	753
Bsh1236I	CGCG	2 cut(s)	9, 383
Bsh1285I	CGRYCG	1 cut(s)	187
BshFI	GGCC	3 cut(s)	62, 212, 1369
BshNI	GGYRCC	2 cut(s)	219, 1840
BsiEI	CGRYCG	1 cut(s)	187
BsiHKAI	GWGCWC	2 cut(s)	15, 829
BsiSI	CCGG	1 cut(s)	1844
BslFI	GGGAC	1 cut(s)	1347
BslI	CCNNNNNNNGG	4 cut(s)	71, 252, 1685, 1850
BsmAI	GTCTC	1 cut(s)	87
BsmFI	GGGAC	1 cut(s)	1347
BsnI	GGCC	3 cut(s)	62, 212, 1369
Bsp1286I	GDGCHC	2 cut(s)	15, 829
Bsp1407I	TGTACA	1 cut(s)	1952
Bsp143I	GATC	3 cut(s)	1669, 1900, 1912
Bsp68I	TCGCGA	1 cut(s)	9
BspACI	CCGC	5 cut(s)	59, 183, 383, 593, 1238
BspANI	GGCC	3 cut(s)	62, 212, 1369
BspCNI	CTCAG	3 cut(s)	442, 733, 958
BspFNI	CGCG	2 cut(s)	9, 383
BspLI	GGNNCC	4 cut(s)	63, 221, 634, 1842
BspMI	ACCTGC	2 cut(s)	1073, 1412
BspPI	GGATC	1 cut(s)	1664
BspQI	GCTCTTC	2 cut(s)	938, 1122
BspT107I	GGYRCC	2 cut(s)	219, 1840
BspTI	CTTAAG	1 cut(s)	224
BsrGI	TGTACA	1 cut(s)	1952
BsrI	ACTGG	4 cut(s)	96, 246, 668, 1184
BssECI	CCNNGG	4 cut(s)	1008, 1364, 1821, 1844
BssMI	GATC	3 cut(s)	1669, 1900, 1912
BssNAI	GTATAC	1 cut(s)	1618
BssNI	GRCGYC	1 cut(s)	626
BssSI	CACGAG	1 cut(s)	323
Bst1107I	GTATAC	1 cut(s)	1618
Bst2BI	CACGAG	1 cut(s)	323
Bst2UI	CCWGG	2 cut(s)	1009, 1366
Bst4CI	ACNGT	1 cut(s)	1264
Bst6I	CTCTTC	3 cut(s)	412, 938, 1122
BstACI	GRCGYC	1 cut(s)	626
BstAFI	CTTAAG	1 cut(s)	224
BstAUI	TGTACA	1 cut(s)	1952
BstC8I	GCNNGC	1 cut(s)	11
BstDEI	CTNAG	5 cut(s)	429, 679, 741, 842, 966
BstDSI	CCRYGG	1 cut(s)	1821
BstF5I	GGATG	9 cut(s)	343, 523, 565, 613, 1054, 1690, 1831, 1854, 1972
BstFNI	CGCG	2 cut(s)	9, 383
BstHHI	GCGC	2 cut(s)	383, 1303
BstKTI	GATC	3 cut(s)	1672, 1903, 1915
BstMAI	GTCTC	1 cut(s)	87
BstMBI	GATC	3 cut(s)	1669, 1900, 1912
BstMCI	CGRYCG	1 cut(s)	187
BstMWI	GCNNNNNNNGC	3 cut(s)	15, 824, 1129
BstNI	CCWGG	2 cut(s)	1009, 1366
BstNSI	RCATGY	1 cut(s)	299
BstSCI	CCNGG	3 cut(s)	1007, 1364, 1843
BstSFI	CTRYAG	2 cut(s)	864, 1305
BstUI	CGCG	2 cut(s)	9, 383
BstV1I	GCAGC	3 cut(s)	50, 1442, 1445
BstZ17I	GTATAC	1 cut(s)	1618
Bsu36I	CCTNAGG	1 cut(s)	429
BsuRI	GGCC	3 cut(s)	62, 212, 1369
BtgI	CCRYGG	1 cut(s)	1821
BtsCI	GGATG	9 cut(s)	343, 523, 565, 613, 1054, 1690, 1831, 1854, 1972
BtsI	GCAGTG	2 cut(s)	741, 955
BtsIMutI	CAGTG	2 cut(s)	741, 955
BtuMI	TCGCGA	1 cut(s)	9
BveI	ACCTGC	2 cut(s)	1073, 1412
Cac8I	GCNNGC	1 cut(s)	11
CaiI	CAGNNNCTG	1 cut(s)	740
CfoI	GCGC	2 cut(s)	383, 1303
Cfr13I	GGNCC	2 cut(s)	61, 633
CseI	GACGC	1 cut(s)	491
Csp6I	GTAC	6 cut(s)	220, 601, 676, 838, 1787, 1953
CspCI	CAANNNNNGTGG	2 cut(s)	1662, 1697
CviAII	CATG	8 cut(s)	296, 757, 763, 926, 1538, 1561, 1910, 1921
CviQI	GTAC	6 cut(s)	220, 601, 676, 838, 1787, 1953
DdeI	CTNAG	5 cut(s)	429, 679, 741, 842, 966
DpnI	GATC	3 cut(s)	1671, 1902, 1914
DpnII	GATC	3 cut(s)	1669, 1900, 1912
DraI	TTTAAA	1 cut(s)	934
DrdI	GACNNNNNNGTC	1 cut(s)	631
DseDI	GACNNNNNNGTC	1 cut(s)	631
EaeI	YGGCCR	1 cut(s)	210
Eam1104I	CTCTTC	3 cut(s)	412, 938, 1122
EarI	CTCTTC	3 cut(s)	412, 938, 1122
Ecl136II	GAGCTC	1 cut(s)	13
Eco24I	GRGCYC	1 cut(s)	15
Eco32I	GATATC	1 cut(s)	1405
Eco47I	GGWCC	1 cut(s)	633
Eco53kI	GAGCTC	1 cut(s)	13
Eco57I	CTGAAG	1 cut(s)	527
Eco81I	CCTNAGG	1 cut(s)	429
EcoICRI	GAGCTC	1 cut(s)	13
EcoRII	CCWGG	2 cut(s)	1007, 1364
EcoRV	GATATC	1 cut(s)	1405
EcoT38I	GRGCYC	1 cut(s)	15
FaeI	CATG	8 cut(s)	299, 760, 766, 929, 1541, 1564, 1913, 1924
FalI	AAGNNNNNCTT	4 cut(s)	296, 328, 1020, 1052
FaqI	GGGAC	1 cut(s)	1347
FatI	CATG	8 cut(s)	295, 756, 762, 925, 1537, 1560, 1909, 1920
FauNDI	CATATG	1 cut(s)	1353
FbaI	TGATCA	1 cut(s)	1900
FblI	GTMKAC	3 cut(s)	1167, 1617, 1971
Fnu4HI	GCNGC	4 cut(s)	39, 60, 1456, 1459
FokI	GGATG	8 cut(s)	350, 510, 572, 620, 1061, 1697, 1838, 1861
FriOI	GRGCYC	1 cut(s)	15
Fsp4HI	GCNGC	4 cut(s)	39, 60, 1456, 1459
FspBI	CTAG	2 cut(s)	45, 882
GlaI	GCGC	2 cut(s)	382, 1302
GluI	GCNGC	4 cut(s)	39, 60, 1456, 1459
HaeIII	GGCC	3 cut(s)	62, 212, 1369
HapII	CCGG	1 cut(s)	1844
HgaI	GACGC	1 cut(s)	491
HhaI	GCGC	2 cut(s)	383, 1303
Hin1I	GRCGYC	1 cut(s)	626
Hin1II	CATG	8 cut(s)	299, 760, 766, 929, 1541, 1564, 1913, 1924
Hin6I	GCGC	2 cut(s)	381, 1301
HinP1I	GCGC	2 cut(s)	381, 1301
HincII	GTYRAC	1 cut(s)	645
HindII	GTYRAC	1 cut(s)	645
HinfI	GANTC	9 cut(s)	22, 80, 514, 536, 982, 1040, 1522, 1541, 1810
HpaII	CCGG	1 cut(s)	1844
HphI	GGTGA	2 cut(s)	89, 397
Hpy166II	GTNNAC	5 cut(s)	645, 1168, 1344, 1618, 1972
Hpy8I	GTNNAC	5 cut(s)	645, 1168, 1344, 1618, 1972
HpyAV	CCTTC	3 cut(s)	677, 795, 844
HpyCH4III	ACNGT	1 cut(s)	1264
HpyCH4IV	ACGT	2 cut(s)	170, 626
HpyCH4V	TGCA	9 cut(s)	239, 322, 734, 796, 1027, 1421, 1515, 1798, 1856
HpyF10VI	GCNNNNNNNGC	3 cut(s)	15, 824, 1129
HpyF3I	CTNAG	5 cut(s)	429, 679, 741, 842, 966
HpySE526I	ACGT	2 cut(s)	170, 626
Hsp92I	GRCGYC	1 cut(s)	626
Hsp92II	CATG	8 cut(s)	299, 760, 766, 929, 1541, 1564, 1913, 1924
HspAI	GCGC	2 cut(s)	381, 1301
KpnI	GGTACC	1 cut(s)	223
Ksp22I	TGATCA	1 cut(s)	1900
Kzo9I	GATC	3 cut(s)	1669, 1900, 1912
LguI	GCTCTTC	2 cut(s)	938, 1122
LmnI	GCTCC	3 cut(s)	520, 1240, 1926
Lsp1109I	GCAGC	3 cut(s)	50, 1442, 1445
LweI	GCATC	6 cut(s)	489, 532, 1129, 1288, 1387, 1524
MaeI	CTAG	2 cut(s)	45, 882
MaeII	ACGT	2 cut(s)	170, 626
MaeIII	GTNAC	5 cut(s)	49, 166, 1225, 1489, 1757
MalI	GATC	3 cut(s)	1671, 1902, 1914
MboI	GATC	3 cut(s)	1669, 1900, 1912
MfeI	CAATTG	1 cut(s)	1118
MhlI	GDGCHC	2 cut(s)	15, 829
MlyI	GAGTC	4 cut(s)	89, 545, 1049, 1516
MroXI	GAANNNNTTC	2 cut(s)	26, 1883
MslI	CAYNNNNRTG	3 cut(s)	761, 1755, 1908
MspCI	CTTAAG	1 cut(s)	224
MspI	CCGG	1 cut(s)	1844
MspR9I	CCNGG	3 cut(s)	1009, 1366, 1845
MunI	CAATTG	1 cut(s)	1118
MvaI	CCWGG	2 cut(s)	1009, 1366
MvnI	CGCG	2 cut(s)	9, 383
MwoI	GCNNNNNNNGC	3 cut(s)	15, 824, 1129
NciI	CCSGG	1 cut(s)	1845
NdeI	CATATG	1 cut(s)	1353
NdeII	GATC	3 cut(s)	1669, 1900, 1912
NlaIII	CATG	8 cut(s)	299, 760, 766, 929, 1541, 1564, 1913, 1924
NlaIV	GGNNCC	4 cut(s)	63, 221, 634, 1842
NmuCI	GTSAC	3 cut(s)	49, 166, 1757
NruI	TCGCGA	1 cut(s)	9
NspI	RCATGY	1 cut(s)	299
PaqCI	CACCTGC	1 cut(s)	1412
PciI	ACATGT	1 cut(s)	295
PciSI	GCTCTTC	2 cut(s)	938, 1122
PdmI	GAANNNNTTC	2 cut(s)	26, 1883
PfeI	GAWTC	5 cut(s)	22, 514, 982, 1541, 1810
PflMI	CCANNNNNTGG	1 cut(s)	252
PkrI	GCNGC	4 cut(s)	40, 61, 1457, 1460
PleI	GAGTC	4 cut(s)	88, 544, 1048, 1516
PpsI	GAGTC	4 cut(s)	88, 544, 1048, 1516
PscI	ACATGT	1 cut(s)	295
PshBI	ATTAAT	1 cut(s)	1860
Psp124BI	GAGCTC	1 cut(s)	15
Psp6I	CCWGG	2 cut(s)	1007, 1364
PspGI	CCWGG	2 cut(s)	1007, 1364
PspN4I	GGNNCC	4 cut(s)	63, 221, 634, 1842
PspPI	GGNCC	2 cut(s)	61, 633
PstNI	CAGNNNCTG	1 cut(s)	740
RruI	TCGCGA	1 cut(s)	9
RsaI	GTAC	6 cut(s)	221, 602, 677, 839, 1788, 1954
RsaNI	GTAC	6 cut(s)	220, 601, 676, 838, 1787, 1953
RseI	CAYNNNNRTG	3 cut(s)	761, 1755, 1908
SacI	GAGCTC	1 cut(s)	15
SapI	GCTCTTC	2 cut(s)	938, 1122
SatI	GCNGC	4 cut(s)	39, 60, 1456, 1459
Sau3AI	GATC	3 cut(s)	1669, 1900, 1912
Sau96I	GGNCC	2 cut(s)	61, 633
ScaI	AGTACT	1 cut(s)	677
SchI	GAGTC	4 cut(s)	89, 545, 1049, 1516
ScrFI	CCNGG	3 cut(s)	1009, 1366, 1845
SduI	GDGCHC	2 cut(s)	15, 829
SfaNI	GCATC	6 cut(s)	489, 532, 1129, 1288, 1387, 1524
SfcI	CTRYAG	2 cut(s)	864, 1305
SinI	GGWCC	1 cut(s)	633
SmiMI	CAYNNNNRTG	3 cut(s)	761, 1755, 1908
SmlI	CTYRAG	3 cut(s)	224, 1037, 1501
SmoI	CTYRAG	3 cut(s)	224, 1037, 1501
SsiI	CCGC	5 cut(s)	59, 183, 383, 593, 1238
SspMI	CTAG	2 cut(s)	45, 882
SstI	GAGCTC	1 cut(s)	15
StyD4I	CCNGG	3 cut(s)	1007, 1364, 1843
TaaI	ACNGT	1 cut(s)	1264
TaiI	ACGT	2 cut(s)	173, 629
TaqI	TCGA	7 cut(s)	30, 449, 480, 534, 539, 1069, 1804
TatI	WGTACW	2 cut(s)	675, 1952
TauI	GCSGC	1 cut(s)	62
TfiI	GAWTC	5 cut(s)	22, 514, 982, 1541, 1810
TscAI	CASTG	2 cut(s)	741, 955
TseFI	GTSAC	3 cut(s)	49, 166, 1757
TseI	GCWGC	3 cut(s)	38, 1455, 1458
Tsp45I	GTSAC	3 cut(s)	49, 166, 1757
TspDTI	ATGAA	8 cut(s)	171, 473, 716, 745, 926, 1032, 1554, 1577
TspGWI	ACGGA	1 cut(s)	87
TspRI	CASTG	2 cut(s)	741, 955
Van91I	CCANNNNNTGG	1 cut(s)	252
Vha464I	CTTAAG	1 cut(s)	224
VpaK11BI	GGWCC	1 cut(s)	633
VspI	ATTAAT	1 cut(s)	1860
XapI	RAATTY	2 cut(s)	489, 530
XceI	RCATGY	1 cut(s)	299
XmiI	GTMKAC	3 cut(s)	1167, 1617, 1971
XmnI	GAANNNNTTC	2 cut(s)	26, 1883
XspI	CTAG	2 cut(s)	45, 882
ZraI	GACGTC	1 cut(s)	627
ZrmI	AGTACT	1 cut(s)	677

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.