Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0012252

Physical Location & Seq

Genomic Coordinates Forward (+)

37132389 .. 37135103

Gene Structure 2715 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000018826

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2391 bp

ATGTGTCTTTTGGACCCCCCTTGGGATGCGAATTCAAAAGGAGGTCATTTTCTTTTAGTTGTAGCAAAACAGAGGGTTTTACATAATGCTCATTTGGAAGCGGCTTCGATGCTCATAAACCTCAGAAGAACAATAGCCATTCCGTTTCACCAAAAACCCTCACTTTCCAAACTCCTCTCAACTCGTACACCAAATCCAAACTCCAACTCGACACAGCCTCTTTCAGAATCCCAACTCAAATCCTTAGTTCTCAGCCGCTACACTCGCGGCAAGTTCACCAACCTCCTCCAAAACGTCGTCGCTTTGCCCGCTGTTCTCCTCACCGCCTGCCAAAACCTCACCGCCCCTCAAACCCAGAATGGCCTCCGCCTCTCCCTTCCCGACTCGGTCTCGAAGCGGTTCTCAATTCACGAAATGGGTCGCGAGCTCTGCGAGAATCGGTTCGATGTGGGAGCTTCTAGTGTCACACTGGCGGCCCCAAGAAACGGAGGTGAGTCTTTGGTCTTACCCAGTTTGAAACTCAAAGTTTTGATTGAAGCTATTAGGATTGTATTGGGCATTGTTTATGATGAACGCTTTGTGACGTTTTCTTATGGCGGTCGTGTTAATATGGGGAGGCACACGGCCATTAGGTACCTTAAGAACTCTGTTGAAAATCCCAGTTGGTGGTTTAGCGTTAGCTTTAATCGGGATAAGTTTGAACAACGACATGTAAATAAGCTATGCTTGTTTATGCACGAGAAAATAGAGGATGAGGTTTTGATTAATATGGTTAAGACATTGTTTGAATGCGGCGCGGTGAGAATTGAATTGGGTAGTTGCTGCTTTGGAAGAGGGTTTCCTCAGGAGAGTGGGTTGAATTCAATTTTAATGAACATTTACTTTAATGGTTTCGATAAAGAAATTCAAGATATGCGTCTCAAAAAGAATCAGGAGCATCCCAAATTCGAGTCGAATGAGCTTGTTTCAAAGGATGGTGTTTTCTATAGGCCTGTCAAGATATATGCGGTTAGGTACTTGGATGAGATATTAGTGATGACGTCCGGGTCCAAAATGTTGACTATGGATTTGAAGAACTGGGTTGTCAAGTACTTAGAAGGGAGTTTGGAATTGATGGTGGATAAGATAAAAACATCTATTCATAGTGCAGTGTCTGAGAAGATTGATTTCATGGGCATGGAATTACAGGCAGTTCCACCTTCGGTGTTGCACCCCCCTATGTCTGAAAAAGCTATTAGAGCACGAAAAAAGTACCTTAGACAGAAGGAAGTCAGAGCTATAGAATTGAAAAATGCTAGAGAGAGGAACAGAAAAAAATTGGGAATGAAAATAATGAGCCATGTTTTTAAAAAGTTGAAGAGCAGTGATGGATTGAAATCTGAGTATCAAATTGAGAATGAAGTCAGAGAAATCTTTAGAACCTGGGCTGATGAAGTGGTGCAAGAGTTCCTTGAGTCATTGGATGAGCGTTGGGATTGGTATCGAAAGCTATCAGCAGGTAATTTTCTGTCTTTAAGACATATTAGACAACAATTACCCGAAGAGCTTGTAGATGCTTATGATAAGTTCCAAGAGCAAGTAGACAAGCATTTGAACCCAGTCAGAGATAGAAAAGCAAGAGAGGAAGAAGAAAGGAGGGTAACAGAAGAAGCGGAACAGAAATATGCCGAGAGAACAGTTGAGGATTTGACAAAGCTCTGTGTGAAAGCTGATGCGCCTATAGAAGTTTTGAGGAAGATGGTCAAGTTAATTGGGTTTACAAATCATATGGGTCGTCCCAGGCCAATCACTTTACTAACTGCTCTTGAAGATGCTGATATCATCAAGTGGTATGCAGGTGTAGGGAGAAGATTGCTTGATTTTTACTGCTGCTGCCATAACTTCAAGACGGTCAAAACTATTGTAACTTATCACTTGAGGTTCTCCTGTATCTTGACTCTAGCTGAAAAGCATGAATCCACCAAAAGTGAAGCCATGAAACATTATACTAAAGATTTGAAAGTCTTTGATATAAATGGAAATCAAGAAGTATACTTTCCCACAGAAAGAGAGGTAAAGATGATGGGAGATAAAAATCTTTCTGATCCAATACCTGTTGATGGGGTATTTTCTTTGGCCCTGATTAGATTAGCTTCTGATGAGCCTCCATATTCTTGTGTTGCTCATTTCTGTGACAGAACTGATACTATTGTTTATCGGGTACGATTATTGCAAAGTCGATTGAATCTGACCCCTGTGGATGATAAGAAATGGGTGCCGGGGATGGGTGCAATTAATGAGAGCCTCCATCTGAAATGCTTCCCTCTTTGTCCTGATCACATACATGATTTATACATGGGGAGCATTACCTTTCAAGATATTGATTGTACATCATTTGTGGATGTAGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000373 GO:0000375 GO:0000377 GO:0000959 GO:0000963 GO:0003674 GO:0003824 GO:0003964 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006259 GO:0006278 GO:0006310 GO:0006314 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0071897 GO:0090304 GO:0090615 GO:0140053 GO:0140097 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Intron_maturas2 RVT_1

Protein Analysis

797

Amino Acids

91.59

Weight (kDa)

8.78

Isoelectric Point (pI)

41.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Intron_maturas2	PF01348	571 - 695	3.1e-17	Type II intron maturase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0012252)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G04050 AT5G04050
fragaria_vesca	FvH4_6g25070
malus_domestica	MD12G1089700.v1.1
prunus_persica	Prupe.7G047200_v2.0.a1 Prupe.7G047200_v2.0.a1 Prupe.7G047200_v2.0.a1
pyrus_communis	pycom12g07560
rosa_chinensis	RchiOBHm_Chr2g0124831
rosa_laevigata	RLG00000018826
rosa_multiflora	Rmu_co8339159.1_g000001
rosa_roxburghii	Rroxscaffold_2G00119620
rosa_rugosa	Rorug02G0253000
rosa_samantha	Rh2AG068200 Rh2AG312500 Rh2BG320700 Rh2CG300700 Rh2DG338000
rosa_wichuraiana	Rw2G025240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1826
AasI	GACNNNNNNGTC	1 cut(s)	1045
AatII	GACGTC	1 cut(s)	1043
Acc36I	ACCTGC	2 cut(s)	1487, 1826
Acc65I	GGTACC	1 cut(s)	633
AccB1I	GGYRCC	2 cut(s)	633, 2254
AccB7I	CCANNNNNTGG	1 cut(s)	666
AccI	GTMKAC	3 cut(s)	1581, 2031, 2385
AccII	CGCG	3 cut(s)	267, 423, 797
AclWI	GGATC	1 cut(s)	2078
AcoI	YGGCCR	1 cut(s)	624
AcsI	RAATTY	4 cut(s)	31, 859, 903, 944
AcyI	GRCGYC	1 cut(s)	1040
AfaI	GTAC	7 cut(s)	187, 635, 1016, 1091, 1253, 2202, 2368
AfiI	CCNNNNNNNGG	5 cut(s)	22, 485, 666, 2099, 2264
AflII	CTTAAG	1 cut(s)	638
AflIII	ACRYGT	1 cut(s)	709
AjnI	CCWGG	2 cut(s)	1421, 1778
Alw21I	GWGCWC	2 cut(s)	429, 1243
Alw26I	GTCTC	2 cut(s)	394, 923
AlwI	GGATC	1 cut(s)	2078
AlwNI	CAGNNNCTG	1 cut(s)	1154
AoxI	GGCC	6 cut(s)	361, 474, 624, 989, 1781, 2115
ApeKI	GCWGC	3 cut(s)	822, 1869, 1872
ApoI	RAATTY	4 cut(s)	31, 859, 903, 944
ArsI	GACNNNNNNTTYG	2 cut(s)	1051, 1083
AseI	ATTAAT	2 cut(s)	765, 2274
Asp700I	GAANNNNTTC	3 cut(s)	398, 440, 2297
Asp718I	GGTACC	1 cut(s)	633
AspLEI	GCGC	2 cut(s)	797, 1717
AspS9I	GGNCC	4 cut(s)	13, 475, 1047, 2116
AsuC2I	CCSGG	2 cut(s)	1045, 2259
AsuHPI	GGTGA	6 cut(s)	140, 268, 313, 331, 503, 811
AvaII	GGWCC	2 cut(s)	13, 1047
AxyI	CCTNAGG	1 cut(s)	843
BaeI	ACNNNNGTAYC	2 cut(s)	2175, 2208
BanI	GGYRCC	2 cut(s)	633, 2254
BanII	GRGCYC	1 cut(s)	429
BauI	CACGAG	1 cut(s)	737
Bbv12I	GWGCWC	2 cut(s)	429, 1243
BbvI	GCAGC	3 cut(s)	809, 1856, 1859
BccI	CCATC	8 cut(s)	968, 1108, 1361, 1732, 2056, 2093, 2257, 2295
BceAI	ACGGC	1 cut(s)	639
BciT130I	CCWGG	2 cut(s)	1423, 1780
BclI	TGATCA	1 cut(s)	2314
BcnI	CCSGG	2 cut(s)	1045, 2259
BcoDI	GTCTC	2 cut(s)	394, 923
BfaI	CTAG	3 cut(s)	459, 1296, 1940
BfmI	CTRYAG	3 cut(s)	985, 1278, 1719
BfrI	CTTAAG	1 cut(s)	638
BfuAI	ACCTGC	2 cut(s)	1487, 1826
BisI	GCNGC	8 cut(s)	102, 256, 268, 474, 793, 823, 1870, 1873
BlsI	GCNGC	8 cut(s)	103, 257, 269, 475, 794, 824, 1871, 1874
BmcAI	AGTACT	1 cut(s)	1091
Bme1390I	CCNGG	4 cut(s)	1045, 1423, 1780, 2259
Bme18I	GGWCC	2 cut(s)	13, 1047
BmgT120I	GGNCC	4 cut(s)	13, 475, 1047, 2116
BmiI	GGNNCC	5 cut(s)	15, 477, 635, 1048, 2256
BmrFI	CCNGG	4 cut(s)	1045, 1423, 1780, 2259
BmrI	ACTGGG	4 cut(s)	504, 654, 1087, 1592
BmsI	GCATC	6 cut(s)	16, 99, 946, 1543, 1702, 1801
BmuI	ACTGGG	4 cut(s)	504, 654, 1087, 1592
BpuEI	CTTGAG	2 cut(s)	1472, 1936
BpuMI	CCSGG	2 cut(s)	1045, 2259
BsaBI	GATNNNNATC	3 cut(s)	1375, 1479, 2073
BsaHI	GRCGYC	1 cut(s)	1040
BsaI	GGTCTC	1 cut(s)	394
BsaJI	CCNNGG	4 cut(s)	20, 1422, 1778, 2258
BsaXI	ACNNNNNCTCC	2 cut(s)	839, 869
Bsc4I	CCNNNNNNNGG	5 cut(s)	22, 485, 666, 2099, 2264
Bse1I	ACTGG	5 cut(s)	474, 510, 660, 1082, 1598
Bse21I	CCTNAGG	1 cut(s)	843
Bse8I	GATNNNNATC	3 cut(s)	1375, 1479, 2073
BseBI	CCWGG	2 cut(s)	1423, 1780
BseDI	CCNNGG	4 cut(s)	20, 1422, 1778, 2258
BseGI	GGATG	9 cut(s)	31, 757, 937, 979, 1027, 1468, 2245, 2268, 2386
BseJI	GATNNNNATC	3 cut(s)	1375, 1479, 2073
BseLI	CCNNNNNNNGG	5 cut(s)	22, 485, 666, 2099, 2264
BseMII	CTCAG	5 cut(s)	136, 265, 857, 1146, 1371
BseNI	ACTGG	5 cut(s)	474, 510, 660, 1082, 1598
BseRI	GAGGAG	3 cut(s)	164, 275, 308
BseXI	GCAGC	3 cut(s)	809, 1856, 1859
BsgI	GTGCAG	1 cut(s)	1167
Bsh1236I	CGCG	3 cut(s)	267, 423, 797
Bsh1285I	CGRYCG	1 cut(s)	601
BshFI	GGCC	6 cut(s)	363, 476, 626, 991, 1783, 2117
BshNI	GGYRCC	2 cut(s)	633, 2254
BsiEI	CGRYCG	1 cut(s)	601
BsiHKAI	GWGCWC	2 cut(s)	429, 1243
BsiSI	CCGG	2 cut(s)	1044, 2258
BslFI	GGGAC	1 cut(s)	1761
BslI	CCNNNNNNNGG	5 cut(s)	22, 485, 666, 2099, 2264
BsmAI	GTCTC	2 cut(s)	394, 923
BsmBI	CGTCTC	1 cut(s)	923
BsmFI	GGGAC	1 cut(s)	1761
BsmI	GAATGC	1 cut(s)	794
BsnI	GGCC	6 cut(s)	363, 476, 626, 991, 1783, 2117
Bso31I	GGTCTC	1 cut(s)	394
Bsp1286I	GDGCHC	2 cut(s)	429, 1243
Bsp1407I	TGTACA	1 cut(s)	2366
Bsp143I	GATC	2 cut(s)	2083, 2314
Bsp68I	TCGCGA	1 cut(s)	423
BspANI	GGCC	6 cut(s)	363, 476, 626, 991, 1783, 2117
BspCNI	CTCAG	5 cut(s)	135, 264, 856, 1147, 1372
BspFNI	CGCG	3 cut(s)	267, 423, 797
BspLI	GGNNCC	5 cut(s)	15, 477, 635, 1048, 2256
BspMI	ACCTGC	2 cut(s)	1487, 1826
BspPI	GGATC	1 cut(s)	2078
BspQI	GCTCTTC	2 cut(s)	1352, 1536
BspT107I	GGYRCC	2 cut(s)	633, 2254
BspTI	CTTAAG	1 cut(s)	638
BspTNI	GGTCTC	1 cut(s)	394
BsrGI	TGTACA	1 cut(s)	2366
BsrI	ACTGG	5 cut(s)	474, 510, 660, 1082, 1598
BssECI	CCNNGG	4 cut(s)	20, 1422, 1778, 2258
BssMI	GATC	2 cut(s)	2083, 2314
BssNAI	GTATAC	1 cut(s)	2032
BssNI	GRCGYC	1 cut(s)	1040
BssSI	CACGAG	1 cut(s)	737
BssT1I	CCWWGG	1 cut(s)	20
Bst1107I	GTATAC	1 cut(s)	2032
Bst2BI	CACGAG	1 cut(s)	737
Bst2UI	CCWGG	2 cut(s)	1423, 1780
Bst4CI	ACNGT	2 cut(s)	1678, 1891
Bst6I	CTCTTC	3 cut(s)	826, 1352, 1536
BstACI	GRCGYC	1 cut(s)	1040
BstAFI	CTTAAG	1 cut(s)	638
BstAUI	TGTACA	1 cut(s)	2366
BstC8I	GCNNGC	3 cut(s)	309, 328, 425
BstDEI	CTNAG	8 cut(s)	122, 244, 251, 843, 1093, 1155, 1256, 1380
BstF5I	GGATG	9 cut(s)	31, 757, 937, 979, 1027, 1468, 2245, 2268, 2386
BstFNI	CGCG	3 cut(s)	267, 423, 797
BstHHI	GCGC	2 cut(s)	797, 1717
BstKTI	GATC	2 cut(s)	2086, 2317
BstMAI	GTCTC	2 cut(s)	394, 923
BstMBI	GATC	2 cut(s)	2083, 2314
BstMCI	CGRYCG	1 cut(s)	601
BstMWI	GCNNNNNNNGC	4 cut(s)	264, 308, 429, 1238
BstNI	CCWGG	2 cut(s)	1423, 1780
BstNSI	RCATGY	1 cut(s)	713
BstSCI	CCNGG	4 cut(s)	1043, 1421, 1778, 2257
BstSFI	CTRYAG	3 cut(s)	985, 1278, 1719
BstUI	CGCG	3 cut(s)	267, 423, 797
BstV1I	GCAGC	3 cut(s)	809, 1856, 1859
BstZ17I	GTATAC	1 cut(s)	2032
Bsu36I	CCTNAGG	1 cut(s)	843
BsuRI	GGCC	6 cut(s)	363, 476, 626, 991, 1783, 2117
BtsCI	GGATG	9 cut(s)	31, 757, 937, 979, 1027, 1468, 2245, 2268, 2386
BtsI	GCAGTG	2 cut(s)	1155, 1369
BtsIMutI	CAGTG	3 cut(s)	467, 1155, 1369
BtuMI	TCGCGA	1 cut(s)	423
BveI	ACCTGC	2 cut(s)	1487, 1826
Cac8I	GCNNGC	3 cut(s)	309, 328, 425
CaiI	CAGNNNCTG	1 cut(s)	1154
CfoI	GCGC	2 cut(s)	797, 1717
Cfr13I	GGNCC	4 cut(s)	13, 475, 1047, 2116
CseI	GACGC	1 cut(s)	905
Csp6I	GTAC	7 cut(s)	186, 634, 1015, 1090, 1252, 2201, 2367
CviAII	CATG	8 cut(s)	710, 1171, 1177, 1340, 1952, 1975, 2324, 2335
CviQI	GTAC	7 cut(s)	186, 634, 1015, 1090, 1252, 2201, 2367
DdeI	CTNAG	8 cut(s)	122, 244, 251, 843, 1093, 1155, 1256, 1380
DpnI	GATC	2 cut(s)	2085, 2316
DpnII	GATC	2 cut(s)	2083, 2314
DraI	TTTAAA	1 cut(s)	1348
DrdI	GACNNNNNNGTC	1 cut(s)	1045
DseDI	GACNNNNNNGTC	1 cut(s)	1045
EaeI	YGGCCR	1 cut(s)	624
Eam1104I	CTCTTC	3 cut(s)	826, 1352, 1536
EarI	CTCTTC	3 cut(s)	826, 1352, 1536
EciI	GGCGGA	1 cut(s)	356
Ecl136II	GAGCTC	1 cut(s)	427
Eco130I	CCWWGG	1 cut(s)	20
Eco147I	AGGCCT	1 cut(s)	991
Eco24I	GRGCYC	1 cut(s)	429
Eco31I	GGTCTC	1 cut(s)	394
Eco32I	GATATC	1 cut(s)	1819
Eco47I	GGWCC	2 cut(s)	13, 1047
Eco53kI	GAGCTC	1 cut(s)	427
Eco81I	CCTNAGG	1 cut(s)	843
EcoICRI	GAGCTC	1 cut(s)	427
EcoRI	GAATTC	2 cut(s)	31, 859
EcoRII	CCWGG	2 cut(s)	1421, 1778
EcoRV	GATATC	1 cut(s)	1819
EcoT14I	CCWWGG	1 cut(s)	20
EcoT38I	GRGCYC	1 cut(s)	429
ErhI	CCWWGG	1 cut(s)	20
Esp3I	CGTCTC	1 cut(s)	923
FaeI	CATG	8 cut(s)	713, 1174, 1180, 1343, 1955, 1978, 2327, 2338
FalI	AAGNNNNNCTT	4 cut(s)	710, 742, 1434, 1466
FaqI	GGGAC	1 cut(s)	1761
FatI	CATG	8 cut(s)	709, 1170, 1176, 1339, 1951, 1974, 2323, 2334
FauI	CCCGC	1 cut(s)	316
FauNDI	CATATG	1 cut(s)	1767
FbaI	TGATCA	1 cut(s)	2314
FblI	GTMKAC	3 cut(s)	1581, 2031, 2385
Fnu4HI	GCNGC	8 cut(s)	102, 256, 268, 474, 793, 823, 1870, 1873
FokI	GGATG	8 cut(s)	38, 764, 924, 986, 1034, 1475, 2252, 2275
FriOI	GRGCYC	1 cut(s)	429
Fsp4HI	GCNGC	8 cut(s)	102, 256, 268, 474, 793, 823, 1870, 1873
FspBI	CTAG	3 cut(s)	459, 1296, 1940
GlaI	GCGC	2 cut(s)	796, 1716
GluI	GCNGC	8 cut(s)	102, 256, 268, 474, 793, 823, 1870, 1873
HaeIII	GGCC	6 cut(s)	363, 476, 626, 991, 1783, 2117
HapII	CCGG	2 cut(s)	1044, 2258
HgaI	GACGC	1 cut(s)	905
HhaI	GCGC	2 cut(s)	797, 1717
Hin1I	GRCGYC	1 cut(s)	1040
Hin1II	CATG	8 cut(s)	713, 1174, 1180, 1343, 1955, 1978, 2327, 2338
Hin6I	GCGC	2 cut(s)	795, 1715
HinP1I	GCGC	2 cut(s)	795, 1715
HincII	GTYRAC	1 cut(s)	1059
HindII	GTYRAC	1 cut(s)	1059
HpaII	CCGG	2 cut(s)	1044, 2258
HphI	GGTGA	6 cut(s)	140, 268, 313, 331, 503, 811
Hpy166II	GTNNAC	7 cut(s)	188, 276, 1059, 1582, 1758, 2032, 2386
Hpy8I	GTNNAC	7 cut(s)	188, 276, 1059, 1582, 1758, 2032, 2386
Hpy99I	CGWCG	2 cut(s)	299, 302
HpyAV	CCTTC	4 cut(s)	386, 1091, 1209, 1258
HpyCH4III	ACNGT	2 cut(s)	1678, 1891
HpyCH4IV	ACGT	3 cut(s)	294, 584, 1040
HpyCH4V	TGCA	7 cut(s)	736, 1148, 1210, 1441, 1835, 2212, 2270
HpyF10VI	GCNNNNNNNGC	4 cut(s)	264, 308, 429, 1238
HpyF3I	CTNAG	8 cut(s)	122, 244, 251, 843, 1093, 1155, 1256, 1380
HpySE526I	ACGT	3 cut(s)	294, 584, 1040
Hsp92I	GRCGYC	1 cut(s)	1040
Hsp92II	CATG	8 cut(s)	713, 1174, 1180, 1343, 1955, 1978, 2327, 2338
HspAI	GCGC	2 cut(s)	795, 1715
KpnI	GGTACC	1 cut(s)	637
Ksp22I	TGATCA	1 cut(s)	2314
Kzo9I	GATC	2 cut(s)	2083, 2314
LguI	GCTCTTC	2 cut(s)	1352, 1536
LmnI	GCTCC	3 cut(s)	452, 934, 2340
Lsp1109I	GCAGC	3 cut(s)	809, 1856, 1859
LweI	GCATC	6 cut(s)	16, 99, 946, 1543, 1702, 1801
MaeI	CTAG	3 cut(s)	459, 1296, 1940
MaeII	ACGT	3 cut(s)	294, 584, 1040
MaeIII	GTNAC	5 cut(s)	463, 580, 1639, 1903, 2171
MalI	GATC	2 cut(s)	2085, 2316
MboI	GATC	2 cut(s)	2083, 2314
MhlI	GDGCHC	2 cut(s)	429, 1243
MlyI	GAGTC	5 cut(s)	377, 503, 959, 1463, 1930
MmeI	TCCRAC	1 cut(s)	228
MroXI	GAANNNNTTC	3 cut(s)	398, 440, 2297
MslI	CAYNNNNRTG	3 cut(s)	1175, 2169, 2322
MspA1I	CMGCKG	1 cut(s)	311
MspCI	CTTAAG	1 cut(s)	638
MspI	CCGG	2 cut(s)	1044, 2258
MspR9I	CCNGG	4 cut(s)	1045, 1423, 1780, 2259
Mva1269I	GAATGC	1 cut(s)	794
MvaI	CCWGG	2 cut(s)	1423, 1780
MvnI	CGCG	3 cut(s)	267, 423, 797
MwoI	GCNNNNNNNGC	4 cut(s)	264, 308, 429, 1238
NciI	CCSGG	2 cut(s)	1045, 2259
NdeI	CATATG	1 cut(s)	1767
NdeII	GATC	2 cut(s)	2083, 2314
NlaIII	CATG	8 cut(s)	713, 1174, 1180, 1343, 1955, 1978, 2327, 2338
NlaIV	GGNNCC	5 cut(s)	15, 477, 635, 1048, 2256
NmeAIII	GCCGAG	1 cut(s)	1693
NmuCI	GTSAC	3 cut(s)	463, 580, 2171
NruI	TCGCGA	1 cut(s)	423
NspI	RCATGY	1 cut(s)	713
PaqCI	CACCTGC	1 cut(s)	1826
PceI	AGGCCT	1 cut(s)	991
PciI	ACATGT	1 cut(s)	709
PciSI	GCTCTTC	2 cut(s)	1352, 1536
PctI	GAATGC	1 cut(s)	794
PdmI	GAANNNNTTC	3 cut(s)	398, 440, 2297
PfeI	GAWTC	5 cut(s)	227, 436, 928, 1955, 2224
PflFI	GACNNNGTC	1 cut(s)	386
PflMI	CCANNNNNTGG	1 cut(s)	666
PkrI	GCNGC	8 cut(s)	103, 257, 269, 475, 794, 824, 1871, 1874
PleI	GAGTC	5 cut(s)	377, 502, 958, 1462, 1930
PpsI	GAGTC	5 cut(s)	377, 502, 958, 1462, 1930
PscI	ACATGT	1 cut(s)	709
PshBI	ATTAAT	2 cut(s)	765, 2274
Psp124BI	GAGCTC	1 cut(s)	429
Psp6I	CCWGG	2 cut(s)	1421, 1778
PspGI	CCWGG	2 cut(s)	1421, 1778
PspN4I	GGNNCC	5 cut(s)	15, 477, 635, 1048, 2256
PspPI	GGNCC	4 cut(s)	13, 475, 1047, 2116
PstNI	CAGNNNCTG	1 cut(s)	1154
PsyI	GACNNNGTC	1 cut(s)	386
RruI	TCGCGA	1 cut(s)	423
RsaI	GTAC	7 cut(s)	187, 635, 1016, 1091, 1253, 2202, 2368
RsaNI	GTAC	7 cut(s)	186, 634, 1015, 1090, 1252, 2201, 2367
RseI	CAYNNNNRTG	3 cut(s)	1175, 2169, 2322
SacI	GAGCTC	1 cut(s)	429
SapI	GCTCTTC	2 cut(s)	1352, 1536
SatI	GCNGC	8 cut(s)	102, 256, 268, 474, 793, 823, 1870, 1873
Sau3AI	GATC	2 cut(s)	2083, 2314
Sau96I	GGNCC	4 cut(s)	13, 475, 1047, 2116
ScaI	AGTACT	1 cut(s)	1091
SchI	GAGTC	5 cut(s)	377, 503, 959, 1463, 1930
ScrFI	CCNGG	4 cut(s)	1045, 1423, 1780, 2259
SduI	GDGCHC	2 cut(s)	429, 1243
SfaNI	GCATC	6 cut(s)	16, 99, 946, 1543, 1702, 1801
SfcI	CTRYAG	3 cut(s)	985, 1278, 1719
SinI	GGWCC	2 cut(s)	13, 1047
SmiMI	CAYNNNNRTG	3 cut(s)	1175, 2169, 2322
SmlI	CTYRAG	3 cut(s)	638, 1451, 1915
SmoI	CTYRAG	3 cut(s)	638, 1451, 1915
SseBI	AGGCCT	1 cut(s)	991
SspMI	CTAG	3 cut(s)	459, 1296, 1940
SstI	GAGCTC	1 cut(s)	429
StuI	AGGCCT	1 cut(s)	991
StyD4I	CCNGG	4 cut(s)	1043, 1421, 1778, 2257
StyI	CCWWGG	1 cut(s)	20
TaaI	ACNGT	2 cut(s)	1678, 1891
TaiI	ACGT	3 cut(s)	297, 587, 1043
TaqI	TCGA	9 cut(s)	107, 209, 392, 444, 894, 948, 953, 1483, 2218
TaqII	GACCGA	1 cut(s)	376
TatI	WGTACW	2 cut(s)	1089, 2366
TauI	GCSGC	5 cut(s)	104, 258, 270, 476, 795
TfiI	GAWTC	5 cut(s)	227, 436, 928, 1955, 2224
TscAI	CASTG	3 cut(s)	474, 1155, 1369
TseFI	GTSAC	3 cut(s)	463, 580, 2171
TseI	GCWGC	3 cut(s)	822, 1869, 1872
Tsp45I	GTSAC	3 cut(s)	463, 580, 2171
TspDTI	ATGAA	9 cut(s)	585, 887, 1130, 1159, 1340, 1413, 1446, 1968, 1991
TspGWI	ACGGA	2 cut(s)	132, 501
TspRI	CASTG	3 cut(s)	474, 1155, 1369
Tth111I	GACNNNGTC	1 cut(s)	386
Van91I	CCANNNNNTGG	1 cut(s)	666
Vha464I	CTTAAG	1 cut(s)	638
VpaK11BI	GGWCC	2 cut(s)	13, 1047
VspI	ATTAAT	2 cut(s)	765, 2274
XapI	RAATTY	4 cut(s)	31, 859, 903, 944
XceI	RCATGY	1 cut(s)	713
XmiI	GTMKAC	3 cut(s)	1581, 2031, 2385
XmnI	GAANNNNTTC	3 cut(s)	398, 440, 2297
XspI	CTAG	3 cut(s)	459, 1296, 1940
ZraI	GACGTC	1 cut(s)	1041
ZrmI	AGTACT	1 cut(s)	1091

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.