MD12G1092700.v1.1

Belongs to the FPP GGPP synthase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013148
Physical Location & Seq
Forward (+)
11921468 .. 11922283
816 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1092700.v1.1.491

Sequence Viewer

Length: 816 bp
ATGGCTTTTTTCTCGATGATATCGGCATCATCCCAACTCTACATTCCAAAGAAGCTAGCTTTTCCAATCCGGTGCTCCTCCGCTTCATCGTCGCCGTCTATTTCAACCCAATCGAGCTCCCTCAAGTTCGATTTGAAAACCTACTGGACTACCCTGATCGCCGACATGAATCAGAAGCTTCACGAAGCCATTCCGATTCGGTACCCAGAGCAGATCTATCAATCCATGCGGTACTCCGTCCTCGCTGATGGCGCCAAGCGAGCCTCACCGGTCATGTGCGTCGCAGCCTGTGAACTCTTCGACGGCAACCACCTTGCCGCCTTCCCTATCGCCTGCGCTCTTGAAATGGTACATGCGGCTTCACTCATACACGACGATATTTCGTGCATGGATGACGACCCATCACGGCGAGGCCAGCCCTCGAACCACATAATTTACGGCGATGACATGGCTATTCTCGCCGGGGATGCTCTGTTTCCATTGGGGTTTCAGCACATTGTATCAAACACGCCTTCCAACCTTGTGTCGGAGCCGCGCCTCCTGCGTGTGATCACAGAGATTGCTCAGACCGTGGGTCCCACGGGAATGGCGGCCGGGCAGTTCCTGGACCTTGAAGGCGGGCCAGAGGCGGTGGAATTCGCTCAGGAGAAGAAGTTTGGGGAGATGAGTCAGCGCTCTGCTGTTTGCGGAGAACTGCTGAGGGGTGCTGAAGATGATGAGTTGGATAGGCTGAGGAGGTATGGGAGAGCTGTTGGGGTTTTGTACCGGGTGGTTGACGATATTCTTGAGGAGAAGATGAAGGGCGGAGATGATTAG

Protein Analysis

272

Amino Acids

29.69

Weight (kDa)

5.02

Isoelectric Point (pI)

51.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
polyprenyl_synt PF00348 71 - 263 1.6e-31 Polyprenyl synthetase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013148)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G38460
fragaria_vesca FvH4_2g15810
malus_domestica MD05G1127100.v1.1 MD12G1092700.v1.1
prunus_persica Prupe.8G170800_v2.0.a1
pyrus_communis pycom05g12290 pycom10g11180
rosa_chinensis RchiOBHm_Chr6g0279181
rosa_laevigata RLG00000013157
rosa_multiflora Rmu_sc0014203.1_g000011 Rmu_sc0014536.1_g000006
rosa_roxburghii Rroxscaffold_7G00189050
rosa_rugosa Rorug06G0123500
rosa_samantha Rh6AG231800 Rh6BG236700 Rh6CG238600 Rh6DG230200
rosa_wichuraiana Rw6G020290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 201
AccB1I GGYRCC 2 cut(s) 201, 251
AccII CGCG 1 cut(s) 535
AcoI YGGCCR 1 cut(s) 591
AcsI RAATTY 1 cut(s) 635
AcuI CTGAAG 1 cut(s) 729
AcyI GRCGYC 1 cut(s) 252
AfaI GTAC 4 cut(s) 203, 233, 351, 764
AfeI AGCGCT 1 cut(s) 674
AfiI CCNNNNNNNGG 1 cut(s) 526
AgeI ACCGGT 1 cut(s) 268
AgsI TTSAA 4 cut(s) 105, 136, 344, 614
AhdI GACNNNNNGTC 1 cut(s) 573
AjnI CCWGG 1 cut(s) 603
AjuI GAANNNNNNNTTGG 2 cut(s) 27, 59
AluBI AGCT 5 cut(s) 55, 59, 117, 178, 749
AluI AGCT 5 cut(s) 55, 59, 117, 178, 749
Alw21I GWGCWC 2 cut(s) 77, 119
AlwNI CAGNNNCTG 1 cut(s) 604
Aor51HI AGCGCT 1 cut(s) 674
AoxI GGCC 3 cut(s) 412, 591, 620
ApeKI GCWGC 1 cut(s) 284
ApoI RAATTY 1 cut(s) 635
AsiGI ACCGGT 1 cut(s) 268
Asp700I GAANNNNTTC 1 cut(s) 189
Asp718I GGTACC 1 cut(s) 201
AspLEI GCGC 4 cut(s) 254, 338, 537, 675
AspS9I GGNCC 3 cut(s) 575, 607, 620
AsuC2I CCSGG 3 cut(s) 463, 595, 767
AsuHPI GGTGA 1 cut(s) 258
AsuNHI GCTAGC 1 cut(s) 55
AvaII GGWCC 2 cut(s) 575, 607
BanI GGYRCC 2 cut(s) 201, 251
BanII GRGCYC 1 cut(s) 119
Bbv12I GWGCWC 2 cut(s) 77, 119
BbvCI CCTCAGC 2 cut(s) 698, 731
BbvI GCAGC 1 cut(s) 296
BccI CCATC 2 cut(s) 242, 409
BceAI ACGGC 4 cut(s) 79, 319, 422, 454
BciT130I CCWGG 1 cut(s) 605
BclI TGATCA 1 cut(s) 549
BcnI CCSGG 3 cut(s) 463, 595, 767
BfaI CTAG 1 cut(s) 56
BfoI RGCGCY 2 cut(s) 255, 676
BglII AGATCT 1 cut(s) 213
BisI GCNGC 5 cut(s) 285, 318, 357, 533, 591
BlsI GCNGC 5 cut(s) 286, 319, 358, 534, 592
Bme1390I CCNGG 4 cut(s) 463, 595, 605, 767
Bme18I GGWCC 2 cut(s) 575, 607
BmeRI GACNNNNNGTC 1 cut(s) 573
BmgT120I GGNCC 3 cut(s) 575, 607, 620
BmiI GGNNCC 5 cut(s) 203, 253, 531, 576, 577
BmrFI CCNGG 4 cut(s) 463, 595, 605, 767
BmsI GCATC 2 cut(s) 35, 457
BmtI GCTAGC 1 cut(s) 59
Bpu10I CCTNAGC 3 cut(s) 642, 698, 731
BpuEI CTTGAG 2 cut(s) 107, 806
BpuMI CCSGG 3 cut(s) 463, 595, 767
BsaHI GRCGYC 1 cut(s) 252
BsaJI CCNNGG 3 cut(s) 462, 570, 579
BsaWI WCCGGW 2 cut(s) 69, 268
BsaXI ACNNNNNCTCC 2 cut(s) 638, 668
Bsc4I CCNNNNNNNGG 1 cut(s) 526
Bse118I RCCGGY 1 cut(s) 268
Bse1I ACTGG 1 cut(s) 149
BseBI CCWGG 1 cut(s) 605
BseDI CCNNGG 3 cut(s) 462, 570, 579
BseGI GGATG 3 cut(s) 29, 397, 472
BseLI CCNNNNNNNGG 1 cut(s) 526
BseMII CTCAG 4 cut(s) 578, 656, 689, 722
BseNI ACTGG 1 cut(s) 149
BseRI GAGGAG 3 cut(s) 67, 748, 803
BseX3I CGGCCG 1 cut(s) 591
BseXI GCAGC 1 cut(s) 296
Bsh1236I CGCG 1 cut(s) 535
Bsh1285I CGRYCG 1 cut(s) 594
BshFI GGCC 3 cut(s) 414, 593, 622
BshNI GGYRCC 2 cut(s) 201, 251
BshTI ACCGGT 1 cut(s) 268
BsiEI CGRYCG 1 cut(s) 594
BsiHKAI GWGCWC 2 cut(s) 77, 119
BsiSI CCGG 5 cut(s) 70, 269, 462, 594, 766
BslFI GGGAC 1 cut(s) 561
BslI CCNNNNNNNGG 1 cut(s) 526
BsmFI GGGAC 1 cut(s) 561
BsnI GGCC 3 cut(s) 414, 593, 622
Bsp1286I GDGCHC 2 cut(s) 77, 119
Bsp143I GATC 3 cut(s) 156, 213, 549
BspANI GGCC 3 cut(s) 414, 593, 622
BspCNI CTCAG 4 cut(s) 577, 655, 690, 723
BspFNI CGCG 1 cut(s) 535
BspLI GGNNCC 5 cut(s) 203, 253, 531, 576, 577
BspOI GCTAGC 1 cut(s) 59
BspT107I GGYRCC 2 cut(s) 201, 251
BsrFI RCCGGY 1 cut(s) 268
BsrI ACTGG 1 cut(s) 149
BssAI RCCGGY 1 cut(s) 268
BssECI CCNNGG 3 cut(s) 462, 570, 579
BssMI GATC 3 cut(s) 156, 213, 549
BssNI GRCGYC 1 cut(s) 252
Bst2UI CCWGG 1 cut(s) 605
Bst4CI ACNGT 1 cut(s) 571
Bst6I CTCTTC 1 cut(s) 302
BstACI GRCGYC 1 cut(s) 252
BstC8I GCNNGC 5 cut(s) 57, 261, 334, 416, 620
BstDEI CTNAG 4 cut(s) 564, 642, 698, 731
BstDSI CCRYGG 2 cut(s) 570, 579
BstF5I GGATG 3 cut(s) 29, 397, 472
BstFNI CGCG 1 cut(s) 535
BstH2I RGCGCY 2 cut(s) 255, 676
BstHHI GCGC 4 cut(s) 254, 338, 537, 675
BstKTI GATC 3 cut(s) 159, 216, 552
BstMBI GATC 3 cut(s) 156, 213, 549
BstMCI CGRYCG 1 cut(s) 594
BstMWI GCNNNNNNNGC 6 cut(s) 251, 260, 415, 458, 467, 541
BstNI CCWGG 1 cut(s) 605
BstNSI RCATGY 1 cut(s) 356
BstSCI CCNGG 4 cut(s) 461, 593, 603, 765
BstUI CGCG 1 cut(s) 535
BstV1I GCAGC 1 cut(s) 296
BstX2I RGATCY 1 cut(s) 213
BstXI CCANNNNNNTGG 1 cut(s) 586
BstYI RGATCY 1 cut(s) 213
BstZI CGGCCG 1 cut(s) 591
BsuRI GGCC 3 cut(s) 414, 593, 622
BtgI CCRYGG 2 cut(s) 570, 579
BtgZI GCGATG 1 cut(s) 456
BtsCI GGATG 3 cut(s) 29, 397, 472
Cac8I GCNNGC 5 cut(s) 57, 261, 334, 416, 620
CaiI CAGNNNCTG 1 cut(s) 604
CfoI GCGC 4 cut(s) 254, 338, 537, 675
Cfr10I RCCGGY 1 cut(s) 268
Cfr13I GGNCC 3 cut(s) 575, 607, 620
CseI GACGC 1 cut(s) 268
Csp6I GTAC 4 cut(s) 202, 232, 350, 763
CspAI ACCGGT 1 cut(s) 268
CviAII CATG 6 cut(s) 166, 226, 274, 353, 388, 448
CviQI GTAC 4 cut(s) 202, 232, 350, 763
DdeI CTNAG 4 cut(s) 564, 642, 698, 731
DinI GGCGCC 1 cut(s) 253
DpnI GATC 3 cut(s) 158, 215, 551
DpnII GATC 3 cut(s) 156, 213, 549
DriI GACNNNNNGTC 1 cut(s) 573
EaeI YGGCCR 1 cut(s) 591
EagI CGGCCG 1 cut(s) 591
Eam1104I CTCTTC 1 cut(s) 302
Eam1105I GACNNNNNGTC 1 cut(s) 573
EarI CTCTTC 1 cut(s) 302
Ecl136II GAGCTC 1 cut(s) 117
EclXI CGGCCG 1 cut(s) 591
Eco24I GRGCYC 1 cut(s) 119
Eco32I GATATC 1 cut(s) 21
Eco47I GGWCC 2 cut(s) 575, 607
Eco47III AGCGCT 1 cut(s) 674
Eco52I CGGCCG 1 cut(s) 591
Eco53kI GAGCTC 1 cut(s) 117
Eco57I CTGAAG 1 cut(s) 729
EcoICRI GAGCTC 1 cut(s) 117
EcoO109I RGGNCCY 1 cut(s) 575
EcoRI GAATTC 1 cut(s) 635
EcoRII CCWGG 1 cut(s) 603
EcoRV GATATC 1 cut(s) 21
EcoT38I GRGCYC 1 cut(s) 119
EgeI GGCGCC 1 cut(s) 253
EheI GGCGCC 1 cut(s) 253
FaeI CATG 6 cut(s) 169, 229, 277, 356, 391, 451
FaiI YATR 9 cut(s) 167, 227, 275, 354, 368, 389, 431, 449, 741
FaqI GGGAC 1 cut(s) 561
FatI CATG 6 cut(s) 165, 225, 273, 352, 387, 447
FauI CCCGC 1 cut(s) 611
FbaI TGATCA 1 cut(s) 549
Fnu4HI GCNGC 5 cut(s) 285, 318, 357, 533, 591
FokI GGATG 3 cut(s) 16, 404, 479
FriOI GRGCYC 1 cut(s) 119
Fsp4HI GCNGC 5 cut(s) 285, 318, 357, 533, 591
FspBI CTAG 1 cut(s) 56
GlaI GCGC 4 cut(s) 253, 337, 536, 674
GluI GCNGC 5 cut(s) 285, 318, 357, 533, 591
HaeII RGCGCY 2 cut(s) 255, 676
HaeIII GGCC 3 cut(s) 414, 593, 622
HapII CCGG 5 cut(s) 70, 269, 462, 594, 766
HgaI GACGC 1 cut(s) 268
HhaI GCGC 4 cut(s) 254, 338, 537, 675
Hin1I GRCGYC 1 cut(s) 252
Hin1II CATG 6 cut(s) 169, 229, 277, 356, 391, 451
Hin6I GCGC 4 cut(s) 252, 336, 535, 673
HinP1I GCGC 4 cut(s) 252, 336, 535, 673
HincII GTYRAC 1 cut(s) 775
HindII GTYRAC 1 cut(s) 775
HindIII AAGCTT 1 cut(s) 176
HinfI GANTC 3 cut(s) 169, 196, 667
HpaII CCGG 5 cut(s) 70, 269, 462, 594, 766
HphI GGTGA 1 cut(s) 258
Hpy166II GTNNAC 2 cut(s) 293, 775
Hpy188I TCNGA 4 cut(s) 174, 195, 529, 567
Hpy188III TCNNGA 5 cut(s) 13, 182, 341, 644, 785
Hpy8I GTNNAC 2 cut(s) 293, 775
Hpy99I CGWCG 4 cut(s) 94, 284, 305, 377
HpyAV CCTTC 4 cut(s) 331, 522, 608, 793
HpyCH4III ACNGT 1 cut(s) 571
HpyCH4V TGCA 1 cut(s) 387
HpyF10VI GCNNNNNNNGC 6 cut(s) 251, 260, 415, 458, 467, 541
HpyF3I CTNAG 4 cut(s) 564, 642, 698, 731
Hsp92I GRCGYC 1 cut(s) 252
Hsp92II CATG 6 cut(s) 169, 229, 277, 356, 391, 451
HspAI GCGC 4 cut(s) 252, 336, 535, 673
KasI GGCGCC 1 cut(s) 251
KflI GGGWCCC 1 cut(s) 575
KpnI GGTACC 1 cut(s) 205
Ksp22I TGATCA 1 cut(s) 549
Kzo9I GATC 3 cut(s) 156, 213, 549
LmnI GCTCC 3 cut(s) 80, 122, 529
Lsp1109I GCAGC 1 cut(s) 296
LweI GCATC 2 cut(s) 35, 457
MaeI CTAG 1 cut(s) 56
MalI GATC 3 cut(s) 158, 215, 551
MboI GATC 3 cut(s) 156, 213, 549
MboII GAAGA 4 cut(s) 289, 661, 722, 805
MflI RGATCY 1 cut(s) 213
MhlI GDGCHC 2 cut(s) 77, 119
MluCI AATT 2 cut(s) 432, 635
Mly113I GGCGCC 1 cut(s) 252
MlyI GAGTC 1 cut(s) 676
MmeI TCCRAC 3 cut(s) 507, 540, 702
MroXI GAANNNNTTC 1 cut(s) 189
MslI CAYNNNNRTG 1 cut(s) 584
MspI CCGG 5 cut(s) 70, 269, 462, 594, 766
MspR9I CCNGG 4 cut(s) 463, 595, 605, 767
MvaI CCWGG 1 cut(s) 605
MvnI CGCG 1 cut(s) 535
MwoI GCNNNNNNNGC 6 cut(s) 251, 260, 415, 458, 467, 541
NarI GGCGCC 1 cut(s) 252
NciI CCSGG 3 cut(s) 463, 595, 767
NdeII GATC 3 cut(s) 156, 213, 549
NheI GCTAGC 1 cut(s) 55
NlaIII CATG 6 cut(s) 169, 229, 277, 356, 391, 451
NlaIV GGNNCC 5 cut(s) 203, 253, 531, 576, 577
NspI RCATGY 1 cut(s) 356
PdmI GAANNNNTTC 1 cut(s) 189
PfeI GAWTC 2 cut(s) 169, 196
PfoI TCCNGGA 1 cut(s) 603
PinAI ACCGGT 1 cut(s) 268
PkrI GCNGC 5 cut(s) 286, 319, 358, 534, 592
PleI GAGTC 1 cut(s) 675
PluTI GGCGCC 1 cut(s) 255
PpsI GAGTC 1 cut(s) 675
PpuMI RGGWCCY 1 cut(s) 575
Psp124BI GAGCTC 1 cut(s) 119
Psp5II RGGWCCY 1 cut(s) 575
Psp6I CCWGG 1 cut(s) 603
PspGI CCWGG 1 cut(s) 603
PspN4I GGNNCC 5 cut(s) 203, 253, 531, 576, 577
PspPI GGNCC 3 cut(s) 575, 607, 620
PspPPI RGGWCCY 1 cut(s) 575
PstNI CAGNNNCTG 1 cut(s) 604
PsuI RGATCY 1 cut(s) 213
RsaI GTAC 4 cut(s) 203, 233, 351, 764
RsaNI GTAC 4 cut(s) 202, 232, 350, 763
RseI CAYNNNNRTG 1 cut(s) 584
SacI GAGCTC 1 cut(s) 119
SatI GCNGC 5 cut(s) 285, 318, 357, 533, 591
Sau3AI GATC 3 cut(s) 156, 213, 549
Sau96I GGNCC 3 cut(s) 575, 607, 620
SchI GAGTC 1 cut(s) 676
ScrFI CCNGG 4 cut(s) 463, 595, 605, 767
SduI GDGCHC 2 cut(s) 77, 119
SfaNI GCATC 2 cut(s) 35, 457
SfoI GGCGCC 1 cut(s) 253
SinI GGWCC 2 cut(s) 575, 607
SmiMI CAYNNNNRTG 1 cut(s) 584
SmlI CTYRAG 2 cut(s) 122, 785
SmoI CTYRAG 2 cut(s) 122, 785
Sse9I AATT 2 cut(s) 432, 635
SspDI GGCGCC 1 cut(s) 251
SspMI CTAG 1 cut(s) 56
SstI GAGCTC 1 cut(s) 119
StyD4I CCNGG 4 cut(s) 461, 593, 603, 765
TaaI ACNGT 1 cut(s) 571
TaqI TCGA 5 cut(s) 14, 113, 129, 300, 422
TasI AATT 2 cut(s) 432, 635
TauI GCSGC 4 cut(s) 320, 359, 535, 593
TfiI GAWTC 2 cut(s) 169, 196
TseI GCWGC 1 cut(s) 284
TspDTI ATGAA 3 cut(s) 75, 182, 812
TspGWI ACGGA 1 cut(s) 226
VpaK11BI GGWCC 2 cut(s) 575, 607
XapI RAATTY 1 cut(s) 635
XceI RCATGY 1 cut(s) 356
XmnI GAANNNNTTC 1 cut(s) 189
XspI CTAG 1 cut(s) 56
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.