MD12G1093100.v1.1
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002818
Physical Location & Seq
Reverse (-)
12132337 .. 12141006
8670 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1093100.v1.1.491

Sequence Viewer

Length: 1503 bp
ATGGAGAGAATACACGTCACTGTGCGTGCTCGGCCGCTCTCAGCCGAAGACCCCAAGACCAGCCCTTGGCGCATTTCCGGCAACTCCATCGCCATCCCCAACACTCCAAGTTCGAATTCGGTACCCCCTCAACCTTCTCTCTCCCCTCGGCTTCTCTGCAATTTTCAATATCCTGGGTTAAATGCATTTTTCTCCTCCAATCTTCATGGATTTGTTTTGTTTGGCTTTACAGATCGAATTTTCGGTGAAGATTGTAAGACGTTCGAGGTCTATGAATCAAAAACCAAAAATATAGTCGCTGCCGCCGTTCGCGGCTTCAATGGAATTGTGTTTGCATATGGGCAAACTAACAGTGGGAAAACTCACACCATTCGAGGCTCGGCTACTGAGCCGGGGGTGATTCCCCTTGCAGTGCGCAAAGAGTGTGGGATATATGTTGCTGGATTGCGAGAAGAAATTGTTGCTTCTCCAGAGCAAGTCCTTGACCTAATGGAGTTTGGAGAGTCTCATCGGCATATTGGAGAGACAAATATGAATCTGTACAGTAGTAGGTCCCACACTATTTTTCGCATGATAATTGAAAGCCGAGATAAACACGTAGATGAAGATATTGGTAATTCTTGTGATGTAGTCCGTGTTTCAGTTCTGAATTTAGTGGACCTAGCTGGTTCAGAACGTGCTGCAAAAACCGTTGCAGAAGGTGTTCGGCTCAAAGAGGGTTCTCATATCAATAAAAGCCTTATGACTTTGGGAACTGTCATTAAAAAACTTAGTGAAAGTGCTGAGAGTCAAGGGGGATATGTTCCTTATCGAGATAGCAAACTTACACGCATATTGCAACCTGCTTTGGGTGGAAATACAAACACAACTATAATATGCAACATCACGCTTGCACAGGTTCATGCAGACGAGACTAAAAGCAGTCTCCAATTTGCAAGTCGAGCATTACGTGTCACGAATTGTGCTCATGTCAACGAGGAATCTCATTCAGAACATTGGGTAGAGGAAATCCTCACTCTGCGAAATACGTTATTGCAGGAAAAGAAAGCCCAAGCTGAAAGAGAGAAGATGGTGCAACAGCAAGCTAAGAAAATTGAGAACCTGAGCTCAATGGTGTTGTATTCAAATAGAGATGAGAATCATGATCGCTTGAAAAAGGAGAAGAGCCATGATCCTTGGTGTCCAGGTAAACTTGCAATGGAGACGTTGGCTGAGGTGCCACCCACCATCCAAACGAAGACGTCTGCTGCAAAACCCATGGGACTGAAGCGTGACATCGGACCACTTCTTCCTTTTGAAGAACCGGTGCATGAGATTGAAGTTTCTGAGAATGAGTTTTGCAAAGAAGACGAAGAAAGTAAAAATATCACGTTGGAGGACTGCGGTCTTCCTGATCAGTGTACTTTATTACATATTACTAATAGGAGAAAAGTGCCACCTAGGACGAAAGGCTTACCCTTGGTTTGTACTGTTTCTATTACATTTTGCCATACTGAACTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000070 GO:0000226 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000779 GO:0000793 GO:0000819 GO:0001932 GO:0001934 GO:0002376 GO:0002478 GO:0002495 GO:0002504 GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005819 GO:0005828 GO:0005829 GO:0005856 GO:0005871 GO:0005874 GO:0005875 GO:0005876 GO:0006810 GO:0006890 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007018 GO:0007049 GO:0007051 GO:0007052 GO:0007059 GO:0007079 GO:0007080 GO:0007088 GO:0007346 GO:0008017 GO:0008092 GO:0008150 GO:0008608 GO:0009893 GO:0009987 GO:0010562 GO:0010564 GO:0010604 GO:0010965 GO:0015630 GO:0015631 GO:0016043 GO:0016192 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019220 GO:0019222 GO:0019882 GO:0019884 GO:0019886 GO:0022402 GO:0022607 GO:0030071 GO:0030496 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033047 GO:0033674 GO:0034508 GO:0034622 GO:0042325 GO:0042327 GO:0043085 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043515 GO:0043549 GO:0043933 GO:0044085 GO:0044093 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0045859 GO:0045860 GO:0045937 GO:0048002 GO:0048193 GO:0048285 GO:0048518 GO:0048522 GO:0050000 GO:0050789 GO:0050790 GO:0050794 GO:0051128 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051233 GO:0051234 GO:0051246 GO:0051247 GO:0051276 GO:0051303 GO:0051305 GO:0051310 GO:0051315 GO:0051338 GO:0051347 GO:0051382 GO:0051383 GO:0051640 GO:0051641 GO:0051649 GO:0051656 GO:0051726 GO:0051783 GO:0051983 GO:0060255 GO:0065003 GO:0065004 GO:0065007 GO:0065009 GO:0070013 GO:0070925 GO:0071824 GO:0071840 GO:0072686 GO:0080090 GO:0098687 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099606 GO:0099607 GO:0140014 GO:1901987 GO:1901990 GO:1902099 GO:1902850 GO:1903047 GO:1905818 GO:1990023
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kinesin

Protein Analysis

501

Amino Acids

55.58

Weight (kDa)

6.51

Isoelectric Point (pI)

46.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kinesin PF00225 78 - 139 3.2e-17 Kinesin motor domain
Microtub_bd PF16796 78 - 139 1.1e-07 Microtubule binding
Kinesin PF00225 141 - 316 4.6e-58 Kinesin motor domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1244
Acc16I TGCGCA 1 cut(s) 416
Acc36I ACCTGC 1 cut(s) 850
Acc65I GGTACC 1 cut(s) 121
AccB1I GGYRCC 2 cut(s) 121, 1216
AccB7I CCANNNNNTGG 1 cut(s) 66
AccBSI CCGCTC 1 cut(s) 37
AccII CGCG 1 cut(s) 312
AciI CCGC 4 cut(s) 35, 303, 312, 1383
AclWI GGATC 1 cut(s) 1166
AcoI YGGCCR 1 cut(s) 32
AcsI RAATTY 3 cut(s) 115, 237, 649
AcuI CTGAAG 1 cut(s) 1286
AcyI GRCGYC 1 cut(s) 1241
AfaI GTAC 4 cut(s) 123, 542, 1402, 1468
AfiI CCNNNNNNNGG 2 cut(s) 66, 848
AflIII ACRYGT 3 cut(s) 13, 595, 949
AgeI ACCGGT 1 cut(s) 1303
AgsI TTSAA 7 cut(s) 167, 319, 581, 1125, 1153, 1298, 1319
AjiI CACGTC 1 cut(s) 16
AjnI CCWGG 2 cut(s) 172, 1183
AjuI GAANNNNNNNTTGG 2 cut(s) 100, 132
AluBI AGCT 4 cut(s) 665, 1055, 1085, 1107
AluI AGCT 4 cut(s) 665, 1055, 1085, 1107
Alw21I GWGCWC 3 cut(s) 31, 967, 1109
Alw26I GTCTC 5 cut(s) 510, 518, 905, 929, 1196
AlwI GGATC 1 cut(s) 1166
AoxI GGCC 1 cut(s) 32
ApeKI GCWGC 3 cut(s) 299, 680, 1247
ApoI RAATTY 3 cut(s) 115, 237, 649
ArsI GACNNNNNNTTYG 2 cut(s) 279, 311
AsiGI ACCGGT 1 cut(s) 1303
Asp700I GAANNNNTTC 1 cut(s) 702
Asp718I GGTACC 1 cut(s) 121
AspA2I CCTAGG 1 cut(s) 1439
AspLEI GCGC 2 cut(s) 72, 417
AspS9I GGNCC 3 cut(s) 552, 658, 1280
AsuC2I CCSGG 1 cut(s) 393
AsuHPI GGTGA 2 cut(s) 257, 409
AsuII TTCGAA 1 cut(s) 113
AvaII GGWCC 3 cut(s) 552, 658, 1280
AvrII CCTAGG 1 cut(s) 1439
BanI GGYRCC 2 cut(s) 121, 1216
BanII GRGCYC 1 cut(s) 1109
BbsI GAAGAC 4 cut(s) 54, 1244, 1353, 1379
Bbv12I GWGCWC 3 cut(s) 31, 967, 1109
BbvCI CCTCAGC 1 cut(s) 1212
BbvI GCAGC 3 cut(s) 286, 667, 1234
BccI CCATC 4 cut(s) 95, 101, 1063, 1235
BceAI ACGGC 1 cut(s) 290
BcgI CGANNNNNNTGC 2 cut(s) 70, 104
BciT130I CCWGG 2 cut(s) 174, 1185
BclI TGATCA 1 cut(s) 1393
BcnI CCSGG 1 cut(s) 393
BcoDI GTCTC 5 cut(s) 510, 518, 905, 929, 1196
BfaI CTAG 2 cut(s) 662, 1440
BfuAI ACCTGC 1 cut(s) 850
BisI GCNGC 6 cut(s) 35, 300, 303, 313, 681, 1248
BlnI CCTAGG 1 cut(s) 1439
BlsI GCNGC 6 cut(s) 36, 301, 304, 314, 682, 1249
Bme1390I CCNGG 3 cut(s) 174, 393, 1185
Bme18I GGWCC 3 cut(s) 552, 658, 1280
BmgBI CACGTC 1 cut(s) 16
BmgT120I GGNCC 3 cut(s) 552, 658, 1280
BmiI GGNNCC 3 cut(s) 123, 554, 1218
BmrFI CCNGG 3 cut(s) 174, 393, 1185
BoxI GACNNNNGTC 1 cut(s) 1383
BpiI GAAGAC 4 cut(s) 54, 1244, 1353, 1379
BpmI CTGGAG 1 cut(s) 453
Bpu10I CCTNAGC 2 cut(s) 1103, 1212
Bpu14I TTCGAA 1 cut(s) 113
BpuMI CCSGG 1 cut(s) 393
BsaAI YACGTR 2 cut(s) 598, 950
BsaBI GATNNNNATC 1 cut(s) 1137
BsaHI GRCGYC 1 cut(s) 1241
BsaJI CCNNGG 8 cut(s) 65, 146, 173, 392, 1175, 1257, 1439, 1458
BsaWI WCCGGW 1 cut(s) 1303
Bsc4I CCNNNNNNNGG 2 cut(s) 66, 848
Bse118I RCCGGY 1 cut(s) 1303
Bse3DI GCAATG 1 cut(s) 1203
Bse8I GATNNNNATC 1 cut(s) 1137
BseBI CCWGG 2 cut(s) 174, 1185
BseDI CCNNGG 8 cut(s) 65, 146, 173, 392, 1175, 1257, 1439, 1458
BseGI GGATG 2 cut(s) 93, 1227
BseJI GATNNNNATC 1 cut(s) 1137
BseLI CCNNNNNNNGG 2 cut(s) 66, 848
BseMI GCAATG 1 cut(s) 1203
BseMII CTCAG 6 cut(s) 54, 378, 774, 1094, 1203, 1317
BseRI GAGGAG 1 cut(s) 184
BseX3I CGGCCG 1 cut(s) 32
BseXI GCAGC 3 cut(s) 286, 667, 1234
Bsh1236I CGCG 1 cut(s) 312
Bsh1285I CGRYCG 1 cut(s) 35
BshFI GGCC 1 cut(s) 34
BshNI GGYRCC 2 cut(s) 121, 1216
BshTI ACCGGT 1 cut(s) 1303
BsiEI CGRYCG 1 cut(s) 35
BsiHKAI GWGCWC 3 cut(s) 31, 967, 1109
BsiSI CCGG 3 cut(s) 78, 392, 1304
BslFI GGGAC 2 cut(s) 538, 1275
BslI CCNNNNNNNGG 2 cut(s) 66, 848
BsmAI GTCTC 5 cut(s) 510, 518, 905, 929, 1196
BsmBI CGTCTC 1 cut(s) 1196
BsmFI GGGAC 2 cut(s) 538, 1275
BsnI GGCC 1 cut(s) 34
Bsp119I TTCGAA 1 cut(s) 113
Bsp1286I GDGCHC 3 cut(s) 31, 967, 1109
Bsp1407I TGTACA 1 cut(s) 540
Bsp143I GATC 4 cut(s) 232, 1144, 1171, 1393
Bsp19I CCATGG 1 cut(s) 1257
BspACI CCGC 4 cut(s) 35, 303, 312, 1383
BspANI GGCC 1 cut(s) 34
BspCNI CTCAG 6 cut(s) 53, 379, 775, 1095, 1204, 1318
BspFNI CGCG 1 cut(s) 312
BspHI TCATGA 1 cut(s) 1141
BspLI GGNNCC 3 cut(s) 123, 554, 1218
BspMI ACCTGC 1 cut(s) 850
BspPI GGATC 1 cut(s) 1166
BspQI GCTCTTC 1 cut(s) 1157
BspT104I TTCGAA 1 cut(s) 113
BspT107I GGYRCC 2 cut(s) 121, 1216
BsrBI CCGCTC 1 cut(s) 37
BsrDI GCAATG 1 cut(s) 1203
BsrFI RCCGGY 1 cut(s) 1303
BsrGI TGTACA 1 cut(s) 540
BssAI RCCGGY 1 cut(s) 1303
BssECI CCNNGG 8 cut(s) 65, 146, 173, 392, 1175, 1257, 1439, 1458
BssMI GATC 4 cut(s) 232, 1144, 1171, 1393
BssNI GRCGYC 1 cut(s) 1241
BssT1I CCWWGG 5 cut(s) 65, 1175, 1257, 1439, 1458
Bst2UI CCWGG 2 cut(s) 174, 1185
Bst4CI ACNGT 6 cut(s) 22, 353, 545, 691, 757, 1471
Bst6I CTCTTC 1 cut(s) 1157
BstACI GRCGYC 1 cut(s) 1241
BstAUI TGTACA 1 cut(s) 540
BstBAI YACGTR 2 cut(s) 598, 950
BstBI TTCGAA 1 cut(s) 113
BstC8I GCNNGC 3 cut(s) 27, 891, 1083
BstDEI CTNAG 8 cut(s) 40, 387, 770, 783, 1086, 1103, 1212, 1326
BstDSI CCRYGG 1 cut(s) 1257
BstF5I GGATG 2 cut(s) 93, 1227
BstFNI CGCG 1 cut(s) 312
BstHHI GCGC 2 cut(s) 72, 417
BstKTI GATC 4 cut(s) 235, 1147, 1174, 1396
BstMAI GTCTC 5 cut(s) 510, 518, 905, 929, 1196
BstMBI GATC 4 cut(s) 232, 1144, 1171, 1393
BstMCI CGRYCG 1 cut(s) 35
BstMWI GCNNNNNNNGC 4 cut(s) 31, 69, 78, 941
BstNI CCWGG 2 cut(s) 174, 1185
BstPAI GACNNNNGTC 1 cut(s) 1383
BstSCI CCNGG 3 cut(s) 172, 391, 1183
BstUI CGCG 1 cut(s) 312
BstV1I GCAGC 3 cut(s) 286, 667, 1234
BstV2I GAAGAC 4 cut(s) 54, 1244, 1353, 1379
BstZI CGGCCG 1 cut(s) 32
BsuRI GGCC 1 cut(s) 34
BtgI CCRYGG 1 cut(s) 1257
BtgZI GCGATG 1 cut(s) 73
BtrI CACGTC 1 cut(s) 16
BtsCI GGATG 2 cut(s) 93, 1227
BtsI GCAGTG 1 cut(s) 417
BtsIMutI CAGTG 4 cut(s) 18, 358, 417, 1403
BveI ACCTGC 1 cut(s) 850
Cac8I GCNNGC 3 cut(s) 27, 891, 1083
CciI TCATGA 1 cut(s) 1141
CfoI GCGC 2 cut(s) 72, 417
Cfr10I RCCGGY 1 cut(s) 1303
Cfr13I GGNCC 3 cut(s) 552, 658, 1280
Csp6I GTAC 4 cut(s) 122, 541, 1401, 1467
CspAI ACCGGT 1 cut(s) 1303
CspCI CAANNNNNGTGG 2 cut(s) 406, 441
CviAII CATG 8 cut(s) 206, 571, 902, 968, 1142, 1169, 1258, 1310
CviQI GTAC 4 cut(s) 122, 541, 1401, 1467
DdeI CTNAG 8 cut(s) 40, 387, 770, 783, 1086, 1103, 1212, 1326
DpnI GATC 4 cut(s) 234, 1146, 1173, 1395
DpnII GATC 4 cut(s) 232, 1144, 1171, 1393
EaeI YGGCCR 1 cut(s) 32
EagI CGGCCG 1 cut(s) 32
Eam1104I CTCTTC 1 cut(s) 1157
EarI CTCTTC 1 cut(s) 1157
Ecl136II GAGCTC 1 cut(s) 1107
EclXI CGGCCG 1 cut(s) 32
Eco130I CCWWGG 5 cut(s) 65, 1175, 1257, 1439, 1458
Eco24I GRGCYC 1 cut(s) 1109
Eco47I GGWCC 3 cut(s) 552, 658, 1280
Eco52I CGGCCG 1 cut(s) 32
Eco53kI GAGCTC 1 cut(s) 1107
Eco57I CTGAAG 1 cut(s) 1286
EcoICRI GAGCTC 1 cut(s) 1107
EcoO109I RGGNCCY 1 cut(s) 552
EcoRI GAATTC 1 cut(s) 115
EcoRII CCWGG 2 cut(s) 172, 1183
EcoT14I CCWWGG 5 cut(s) 65, 1175, 1257, 1439, 1458
EcoT22I ATGCAT 1 cut(s) 187
EcoT38I GRGCYC 1 cut(s) 1109
ErhI CCWWGG 5 cut(s) 65, 1175, 1257, 1439, 1458
Esp3I CGTCTC 1 cut(s) 1196
FaeI CATG 8 cut(s) 209, 574, 905, 971, 1145, 1172, 1261, 1313
FaqI GGGAC 2 cut(s) 538, 1275
FatI CATG 8 cut(s) 205, 570, 901, 967, 1141, 1168, 1257, 1309
FauNDI CATATG 1 cut(s) 337
FbaI TGATCA 1 cut(s) 1393
Fnu4HI GCNGC 6 cut(s) 35, 300, 303, 313, 681, 1248
FokI GGATG 2 cut(s) 80, 1214
FriOI GRGCYC 1 cut(s) 1109
Fsp4HI GCNGC 6 cut(s) 35, 300, 303, 313, 681, 1248
FspBI CTAG 2 cut(s) 662, 1440
FspI TGCGCA 1 cut(s) 416
GlaI GCGC 2 cut(s) 71, 416
GluI GCNGC 6 cut(s) 35, 300, 303, 313, 681, 1248
GsuI CTGGAG 1 cut(s) 453
HaeIII GGCC 1 cut(s) 34
HapII CCGG 3 cut(s) 78, 392, 1304
HhaI GCGC 2 cut(s) 72, 417
Hin1I GRCGYC 1 cut(s) 1241
Hin1II CATG 8 cut(s) 209, 574, 905, 971, 1145, 1172, 1261, 1313
Hin6I GCGC 2 cut(s) 70, 415
HinP1I GCGC 2 cut(s) 70, 415
HincII GTYRAC 1 cut(s) 973
HindII GTYRAC 1 cut(s) 973
HinfI GANTC 7 cut(s) 275, 400, 503, 535, 787, 980, 1138
HpaII CCGG 3 cut(s) 78, 392, 1304
HphI GGTGA 2 cut(s) 257, 409
Hpy166II GTNNAC 4 cut(s) 658, 973, 1190, 1401
Hpy188I TCNGA 5 cut(s) 648, 673, 991, 1280, 1327
Hpy188III TCNNGA 5 cut(s) 470, 812, 955, 1142, 1391
Hpy8I GTNNAC 4 cut(s) 658, 973, 1190, 1401
HpyAV CCTTC 2 cut(s) 144, 692
HpyCH4III ACNGT 6 cut(s) 22, 353, 545, 691, 757, 1471
HpyCH4IV ACGT 9 cut(s) 15, 260, 597, 676, 949, 1028, 1205, 1241, 1370
HpyF10VI GCNNNNNNNGC 4 cut(s) 31, 69, 78, 941
HpyF3I CTNAG 8 cut(s) 40, 387, 770, 783, 1086, 1103, 1212, 1326
HpySE526I ACGT 9 cut(s) 15, 260, 597, 676, 949, 1028, 1205, 1241, 1370
Hsp92I GRCGYC 1 cut(s) 1241
Hsp92II CATG 8 cut(s) 209, 574, 905, 971, 1145, 1172, 1261, 1313
HspAI GCGC 2 cut(s) 70, 415
KpnI GGTACC 1 cut(s) 125
Ksp22I TGATCA 1 cut(s) 1393
Kzo9I GATC 4 cut(s) 232, 1144, 1171, 1393
LguI GCTCTTC 1 cut(s) 1157
Lsp1109I GCAGC 3 cut(s) 286, 667, 1234
MaeI CTAG 2 cut(s) 662, 1440
MaeII ACGT 9 cut(s) 15, 260, 597, 676, 949, 1028, 1205, 1241, 1370
MaeIII GTNAC 3 cut(s) 16, 952, 1271
MalI GATC 4 cut(s) 234, 1146, 1173, 1395
MbiI CCGCTC 1 cut(s) 37
MboI GATC 4 cut(s) 232, 1144, 1171, 1393
MhlI GDGCHC 3 cut(s) 31, 967, 1109
MlyI GAGTC 2 cut(s) 512, 796
MmeI TCCRAC 1 cut(s) 1353
Mph1103I ATGCAT 1 cut(s) 187
MroXI GAANNNNTTC 1 cut(s) 702
MseI TTAA 2 cut(s) 179, 762
MslI CAYNNNNRTG 1 cut(s) 600
MspI CCGG 3 cut(s) 78, 392, 1304
MspR9I CCNGG 3 cut(s) 174, 393, 1185
MvaI CCWGG 2 cut(s) 174, 1185
MvnI CGCG 1 cut(s) 312
MwoI GCNNNNNNNGC 4 cut(s) 31, 69, 78, 941
NciI CCSGG 1 cut(s) 393
NcoI CCATGG 1 cut(s) 1257
NdeI CATATG 1 cut(s) 337
NdeII GATC 4 cut(s) 232, 1144, 1171, 1393
NlaIII CATG 8 cut(s) 209, 574, 905, 971, 1145, 1172, 1261, 1313
NlaIV GGNNCC 3 cut(s) 123, 554, 1218
NmeAIII GCCGAG 4 cut(s) 10, 127, 359, 611
NmuCI GTSAC 3 cut(s) 16, 952, 1271
NsbI TGCGCA 1 cut(s) 416
NsiI ATGCAT 1 cut(s) 187
NspV TTCGAA 1 cut(s) 113
PagI TCATGA 1 cut(s) 1141
PciSI GCTCTTC 1 cut(s) 1157
PcsI WCGNNNNNNNCGW 2 cut(s) 303, 946
PdmI GAANNNNTTC 1 cut(s) 702
PfeI GAWTC 5 cut(s) 275, 400, 535, 980, 1138
PflMI CCANNNNNTGG 1 cut(s) 66
PinAI ACCGGT 1 cut(s) 1303
PkrI GCNGC 6 cut(s) 36, 301, 304, 314, 682, 1249
PleI GAGTC 2 cut(s) 511, 795
PpsI GAGTC 2 cut(s) 511, 795
Ppu21I YACGTR 2 cut(s) 598, 950
PpuMI RGGWCCY 1 cut(s) 552
PshAI GACNNNNGTC 1 cut(s) 1383
Psp124BI GAGCTC 1 cut(s) 1109
Psp5II RGGWCCY 1 cut(s) 552
Psp6I CCWGG 2 cut(s) 172, 1183
PspGI CCWGG 2 cut(s) 172, 1183
PspN4I GGNNCC 3 cut(s) 123, 554, 1218
PspPI GGNCC 3 cut(s) 552, 658, 1280
PspPPI RGGWCCY 1 cut(s) 552
RsaI GTAC 4 cut(s) 123, 542, 1402, 1468
RsaNI GTAC 4 cut(s) 122, 541, 1401, 1467
RseI CAYNNNNRTG 1 cut(s) 600
SacI GAGCTC 1 cut(s) 1109
SapI GCTCTTC 1 cut(s) 1157
SaqAI TTAA 2 cut(s) 179, 762
SatI GCNGC 6 cut(s) 35, 300, 303, 313, 681, 1248
Sau3AI GATC 4 cut(s) 232, 1144, 1171, 1393
Sau96I GGNCC 3 cut(s) 552, 658, 1280
SchI GAGTC 2 cut(s) 512, 796
ScrFI CCNGG 3 cut(s) 174, 393, 1185
SduI GDGCHC 3 cut(s) 31, 967, 1109
SfuI TTCGAA 1 cut(s) 113
SinI GGWCC 3 cut(s) 552, 658, 1280
SmiMI CAYNNNNRTG 1 cut(s) 600
SsiI CCGC 4 cut(s) 35, 303, 312, 1383
SspMI CTAG 2 cut(s) 662, 1440
SstI GAGCTC 1 cut(s) 1109
StyD4I CCNGG 3 cut(s) 172, 391, 1183
StyI CCWWGG 5 cut(s) 65, 1175, 1257, 1439, 1458
TaaI ACNGT 6 cut(s) 22, 353, 545, 691, 757, 1471
TaiI ACGT 9 cut(s) 18, 263, 600, 679, 952, 1031, 1208, 1244, 1373
TaqI TCGA 6 cut(s) 113, 235, 264, 373, 811, 940
TatI WGTACW 3 cut(s) 540, 1400, 1466
TauI GCSGC 3 cut(s) 37, 305, 315
TfiI GAWTC 5 cut(s) 275, 400, 535, 980, 1138
Tru1I TTAA 2 cut(s) 179, 762
Tru9I TTAA 2 cut(s) 179, 762
TscAI CASTG 4 cut(s) 25, 358, 417, 1403
TseFI GTSAC 3 cut(s) 16, 952, 1271
TseI GCWGC 3 cut(s) 299, 680, 1247
Tsp45I GTSAC 3 cut(s) 16, 952, 1271
TspDTI ATGAA 5 cut(s) 194, 288, 548, 618, 890
TspGWI ACGGA 1 cut(s) 623
TspRI CASTG 4 cut(s) 25, 358, 417, 1403
Van91I CCANNNNNTGG 1 cut(s) 66
VpaK11BI GGWCC 3 cut(s) 552, 658, 1280
XapI RAATTY 3 cut(s) 115, 237, 649
XmaJI CCTAGG 1 cut(s) 1439
XmnI GAANNNNTTC 1 cut(s) 702
XspI CTAG 2 cut(s) 662, 1440
ZraI GACGTC 1 cut(s) 1242
Zsp2I ATGCAT 1 cut(s) 187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.