MD14G1085100.v1.1
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0002818
Physical Location & Seq
Reverse (-)
10331090 .. 10346039
14950 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1085100.v1.1.491

Sequence Viewer

Length: 3945 bp
ATGGAGAGAATTCACGTCACTGTGCGTGCTCGGCCGCTCTCAGCCGAAGACGCCAAGACCAGCCCTTGGCGCATCTCCGGCAACTCCATCGCCATCCCCAACTACTCCAAGTTCGAATTCGATCGAATTTTCGGTGAAGATTGTAAGACCTTCGAGGTCTATGAATCAAAAACCAAAGATATAGTCGCCGCCGCGGTTCGCGGCTTCAATGGAACCGTGTTTGCATATGGGCAAACTAACAGTGGGAAAACTCACACCATTCGAGGCTCGGCTACTGAACCGGGGGTGATTCCTCTTGCAGTGCGCGATATGTTCGACATTATTCAAGAGGATGTGGACCGGGAATTCCTATTACGAATGTCTTATATGGAGATCTATAATGAGGACATAAATGATTTGTTGGCTCCTGAGCATCGAAAGTTGCAAATTCATGAAAGTCTAGAGCGAGGGATATATGTTGCTGGATTGCGAGAAGAAATTGTTGCCTCTCCAGAGCAAGTCCTTGATCTCATGGAGTTTGGAGAGTCTCATCGGCATATCGGAGAGACAAATATGAATCTGTACAGTAGTAGGTCCCACACTATTTTCCGCATGATAATTGAAAGCCGAGATAAACATGAAGGTGAAGATATTGCTAATTCTTGTGATGCGGTCCGTGTTTCAGTTCTGAATTTAGTGGACCTAGCTGGTTCAGAACGTGCTGCAAAAACTGGTGCAGAAGGTGTTCGGCTCAAAGAGGGTTCCCATATCAATAAAAGCCTTATGACTTTGGGAACTGTCATTAAAAAACTGAGTGAAGGTGCTGAGAGTCAAGGGGGACATGTCCCATATCGAGATAGCAAACTTACGCGCATATTGCAACCTGCTTTGGGTGGAAATGCAAACACAGCTATAATATGCAACATCACTCTTGCACAAATTCATGCAGATGAGACTAAAAGCAGTCTCCAATTTGCAAGTAGAGCATTACGTGTCACGAATTGTGCTCATGTCAATGAGATTCTAACAGATGCTGCTCTTCTCAAGCGTCAAAAGAAAGAAATTGAGGAGCTTCGAGCGAAATTGCAGGAATCTCATTCAGAACATTGGGCAGAGGAAATCCTCAATCTGCGAAATACGTTATTGCAGACTGAGTTAGAGAGGGAGCGTATAGCTTTGGAGTTGGAGGAGGAAAAGAAAGCCCAAGCAGAAAGAGAGAAGATGGTGCAACAACAAGCTAAGAAAATTGAGAACCTGAGTTCAATGGTGTTGTATTCAAATAGAGATGAGAATCGCGACCGCTTGAAAAAGGAGAAGAGGCGTGATACTTGGTGTCCAGGTAAACTTGCAAGGGAGACTTTGGGAGAGGTGCCATCCACCATCCAGTCAAAGGCATCTGCTGCAAAACCTATGAGACTGAAGCGTGACATCGGACCACTTCTTCCTTTTGAAGAACTGGTGCATGAGATTGAAGTTTCTGAGAATGAGTCTTGCAAAGAAGACGAAGAAAGTAATTGTATCACGTTGGAGGACCGTGCTCTTCCTGATCCATGTGCTTTGTTACATGTTACTAATAGGAGAAAAGTGCCACCCAGGACGAAAAGCTTACCCTTGGACAATGAATTAGGAGATCTGCAAGCAGAATACGAAGATTTGCTTATCAAATATGAAACTCAGATACAAATTGATTGCTTGACACGGAAGCTCGCTGAGGCTGATATGTTATCTGGAGCAATGTATAATGATTACTCCAAGTCTTTCCTCAATAAAGGTACCATTAATGGGGACAATAACGGTAGTTTAAGAGAGCCAGAGGTTATTCTTGTGATTAAGCGACTTCAAGAACAGATTAAGATGCTTGAAACAGAGAAGTCTTCAAGCCAGCAAAATCTGGATAGTGTAGTTGAGCTGGCAACTGAGCAAAACATTTGTGCCCGGGAGAAGTTTGACGAGCTCTATGAAGAGCTTCAAGTTGCACGTGAGGAGGCCAGGGTAGCTCGTGATCAACGTACTTGGAACGAATCTGTAACAAAAGTTGATGATGGAAGTTCTGACTTTGTGATCGAGCTTGCAAAGGGGGTCCAAGAAATAATATTGGAAGTTCAAAGCTCAGAAATAGCAATTGAAAGTGTATCTTCAGTTTTGGATGAGGTCTCTAAGAGTTTCTCTGTGCTATTCGATACATTCCTAGATTTCAAGTCTTTAATGTGTGAATTCTCCTTGAAACAAAAAACAATTATCAGTGACCATGAGAAGTTAAACTCTTACATGATGCAGAAAGTTTCTGGACTTGAGACTGAAAAGTTCCTTTTGTACAATCAATCAGTTGATCTTCAGAACCAGATAGAAGAACTGAAACTGCAGACTCAAAATTCCGAACAGTCTTTGAGGGAAATGTTGGAACAACAGGATTTGGAAAAGGGAGAATATATTTCTCATATACAAGTCCTTGAAAAAGAAATATCAGGTTTATCATCTTGTTCTTTGGCCAAGGAAAAGGAAAATCTGCGCAAAGATGTTGAAAAAACAAGAATGAAGTTGAAAGAGACAGAGTGTAAGCTGAAGAATGCCATACAAGAGAAAACCAAACTGGAGGGTGAAAAAGCATCTGCTGAACGAGAAATAAAACGATTGCAGAGTCAGAACTCTCTTCTTGAACGTGACATGAACAAACGTGAGTCACTTGCTGGTAGAAGGCGTGATTCAGTTAATGATAAGGGTTTGAAGACATCGGATCCTAAAAGAGCAAAAAACCTTGCCTTCGAACAGACATTGCAGGAAGAATACAAAAAGATGGAAGTTTATGCTTTTGAAACGGAGACAAAGATTAATTCTCTAGAAGAAGAGTTATCAGCTGTATACAGGGAAAAAGAAGACGCTATGTCCACCAATGATGCTTTAGCATCTGAATTAGAAAACCTGTCTGAAAAACTGAGCACATCAAACTTGGAACTGGAAGCTTTGCAGGAAGAGCTTTTGGCCCTGCAGAGGTTAGAAGAATCTACATTTGAGCAGCAAAAGATGGAAGGTTCCATTAAAATGTTCACTGAAGAAAAGGAAGACTTGGCGATGCAACTTACAGATTCCCTTTTGGAAATGGAGGAGGAAAGAGCAATATGGTCAGCTAAGGAGAAGGCTTCCATTGAAGCAATGGAAGAGAAATCAAAGTTATACAACATGGAGATTACATCATTAACAAGGGAAATATCTGAGGTGAGGAATGAGTTGGACTCTTGTAGACAAGAGTGCAAGGTCCTAAGGGAAAGTTTAACTTGTTGCGAGGAAACAGCTGGACAGAAGACATTCAGCGTGGAGAAGCCTTTGGGGATTGATCAACTGAATAGTGGTGAGAGTATAGATGATATTCTGAGCAAAGAGTCTCAAGAATTGTTGAGCTCAAACTTGGGGACGTGCGGAATTAATGATTATGAGGAGGTGAATGTGCTTCGGAAGGAGCTCAGTTGTCTGAGTAAAGAAAGAGAAGGCTTGTTGATTCAAATAAAAGAGTTATCCGAACTTTCAAATGACCACCAGAGTCTCAACAACCAGCTCCATGTTTTGACAATTGAAAGGGATAATCTAGTGACTCAGATACAAGAGCACCAGAAACTTGCAATTGAAGAAGAAAGTCTTAATAAACGTTATAATGATTTGTTGTTGGAGGCAAAGTTTCAAGTCGAGGAACTGAAAAGGAGAATATCAAGCATGGAGTTAAAGACTCACAAAGATCAAGTTGAAAGCAGCATAGACAAAGCAAAGCTCCGAATGAGGCTTCGAGGGGCACAAGCAAAGTTAGATGCCTTCCGAAGTAGATACAAGGACGCTCTAGACGAGTCCAATCACATGAACAGGAAGTATGAGGAGGCTACAGTCAAGCTAAAGGACCTGTTAGCTTCAAAAGGAGTAGAGGTCCTCGGCCTTAAGAAGCAGCTTGCTGCTGCAAAGAGTGGTCATGAACAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000070 GO:0000226 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000779 GO:0000793 GO:0000819 GO:0001932 GO:0001934 GO:0002376 GO:0002478 GO:0002495 GO:0002504 GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005819 GO:0005828 GO:0005829 GO:0005856 GO:0005871 GO:0005874 GO:0005875 GO:0005876 GO:0006810 GO:0006890 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007018 GO:0007049 GO:0007051 GO:0007052 GO:0007059 GO:0007079 GO:0007080 GO:0007088 GO:0007346 GO:0008017 GO:0008092 GO:0008150 GO:0008608 GO:0009893 GO:0009987 GO:0010562 GO:0010564 GO:0010604 GO:0010965 GO:0015630 GO:0015631 GO:0016043 GO:0016192 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019220 GO:0019222 GO:0019882 GO:0019884 GO:0019886 GO:0022402 GO:0022607 GO:0030071 GO:0030496 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033047 GO:0033674 GO:0034508 GO:0034622 GO:0042325 GO:0042327 GO:0043085 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043515 GO:0043549 GO:0043933 GO:0044085 GO:0044093 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0045859 GO:0045860 GO:0045937 GO:0048002 GO:0048193 GO:0048285 GO:0048518 GO:0048522 GO:0050000 GO:0050789 GO:0050790 GO:0050794 GO:0051128 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051233 GO:0051234 GO:0051246 GO:0051247 GO:0051276 GO:0051303 GO:0051305 GO:0051310 GO:0051315 GO:0051338 GO:0051347 GO:0051382 GO:0051383 GO:0051640 GO:0051641 GO:0051649 GO:0051656 GO:0051726 GO:0051783 GO:0051983 GO:0060255 GO:0065003 GO:0065004 GO:0065007 GO:0065009 GO:0070013 GO:0070925 GO:0071824 GO:0071840 GO:0072686 GO:0080090 GO:0098687 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099606 GO:0099607 GO:0140014 GO:1901987 GO:1901990 GO:1902099 GO:1902850 GO:1903047 GO:1905818 GO:1990023
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kinesin

Protein Analysis

1315

Amino Acids

149.93

Weight (kDa)

5.13

Isoelectric Point (pI)

51.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Microtub_bd PF16796 34 - 134 5.2e-19 Microtubule binding
Kinesin PF00225 35 - 323 3.3e-104 Kinesin motor domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3624
Acc16I TGCGCA 1 cut(s) 2489
Acc36I ACCTGC 1 cut(s) 871
Acc65I GGTACC 1 cut(s) 1751
AccB1I GGYRCC 2 cut(s) 1348, 1751
AccB7I CCANNNNNTGG 1 cut(s) 66
AccBSI CCGCTC 1 cut(s) 37
AccI GTMKAC 2 cut(s) 2838, 3217
AccII CGCG 5 cut(s) 194, 201, 306, 850, 1275
AciI CCGC 9 cut(s) 35, 189, 192, 194, 201, 589, 650, 1279, 3393
AclI AACGTT 1 cut(s) 3619
AclWI GGATC 3 cut(s) 1520, 2708, 2721
AcoI YGGCCR 2 cut(s) 32, 2466
AcsI RAATTY 9 cut(s) 9, 116, 126, 344, 426, 670, 918, 2192, 2350
AcuI CTGAAG 5 cut(s) 1418, 2100, 2297, 2561, 3048
AcvI CACGTG 1 cut(s) 1958
AcyI GRCGYC 1 cut(s) 51
AfaI GTAC 4 cut(s) 563, 1753, 1990, 2294
AfiI CCNNNNNNNGG 5 cut(s) 66, 869, 1369, 1761, 2967
AflII CTTAAG 1 cut(s) 3899
AflIII ACRYGT 3 cut(s) 820, 970, 1543
AhdI GACNNNNNGTC 1 cut(s) 2860
AjiI CACGTC 2 cut(s) 16, 3390
AjnI CCWGG 3 cut(s) 1315, 1571, 1967
AjuI GAANNNNNNNTTGG 2 cut(s) 101, 133
Alw21I GWGCWC 8 cut(s) 31, 988, 1519, 1935, 2918, 3377, 3438, 3582
AlwI GGATC 3 cut(s) 1520, 2708, 2721
AlwNI CAGNNNCTG 1 cut(s) 1013
Ama87I CYCGRG 1 cut(s) 1914
AoxI GGCC 5 cut(s) 32, 1965, 2466, 2958, 3895
ApeKI GCWGC 8 cut(s) 701, 1013, 1379, 2992, 3720, 3907, 3914, 3917
ApoI RAATTY 9 cut(s) 9, 116, 126, 344, 426, 670, 918, 2192, 2350
ArsI GACNNNNNNTTYG 2 cut(s) 168, 200
AseI ATTAAT 3 cut(s) 1758, 2808, 3399
Asp700I GAANNNNTTC 3 cut(s) 723, 1944, 3281
Asp718I GGTACC 1 cut(s) 1751
AspLEI GCGC 4 cut(s) 72, 306, 852, 2490
AsuC2I CCSGG 4 cut(s) 282, 341, 1915, 1916
AsuHPI GGTGA 7 cut(s) 146, 298, 635, 2588, 3205, 3338, 3427
AsuII TTCGAA 2 cut(s) 114, 2742
AvaI CYCGRG 1 cut(s) 1914
AxyI CCTNAGG 1 cut(s) 3236
BaeGI GKGCMC 2 cut(s) 1915, 3763
BalI TGGCCA 1 cut(s) 2468
BamHI GGATCC 1 cut(s) 2713
BanI GGYRCC 2 cut(s) 1348, 1751
BanII GRGCYC 3 cut(s) 1935, 3377, 3438
BauI CACGAG 1 cut(s) 1977
BbrPI CACGTG 1 cut(s) 1958
BbsI GAAGAC 7 cut(s) 54, 1485, 1845, 2711, 2859, 3045, 3284
Bbv12I GWGCWC 8 cut(s) 31, 988, 1519, 1935, 2918, 3377, 3438, 3582
BbvCI CCTCAGC 1 cut(s) 1689
BbvI GCAGC 8 cut(s) 688, 1000, 1366, 3004, 3732, 3901, 3904, 3919
BccI CCATC 8 cut(s) 95, 101, 1195, 1360, 1367, 2015, 2767, 2995
BcgI CGANNNNNNTGC 2 cut(s) 70, 104
BciT130I CCWGG 3 cut(s) 1317, 1573, 1969
BclI TGATCA 2 cut(s) 1981, 3310
BcnI CCSGG 4 cut(s) 282, 341, 1915, 1916
BfaI CTAG 6 cut(s) 440, 683, 2168, 2816, 3560, 3806
BfmI CTRYAG 3 cut(s) 2339, 2963, 3846
BfrI CTTAAG 1 cut(s) 3899
BfuAI ACCTGC 1 cut(s) 871
BglII AGATCT 2 cut(s) 372, 1609
Bme1390I CCNGG 7 cut(s) 282, 341, 1317, 1573, 1915, 1916, 1969
BmeRI GACNNNNNGTC 1 cut(s) 2860
BmeT110I CYCGRG 1 cut(s) 1914
BmgBI CACGTC 2 cut(s) 16, 3390
BmiI GGNNCC 9 cut(s) 214, 405, 575, 742, 1350, 1753, 2060, 2715, 3010
BmrFI CCNGG 7 cut(s) 282, 341, 1317, 1573, 1915, 1916, 1969
BpiI GAAGAC 7 cut(s) 54, 1485, 1845, 2711, 2859, 3045, 3284
BplI GAGNNNNNCTC 2 cut(s) 3194, 3226
BpmI CTGGAG 3 cut(s) 474, 1728, 2591
Bpu10I CCTNAGC 3 cut(s) 408, 1689, 3105
Bpu14I TTCGAA 2 cut(s) 114, 2742
BpuEI CTTGAG 3 cut(s) 1007, 2291, 3345
BpuMI CCSGG 4 cut(s) 282, 341, 1915, 1916
BsaAI YACGTR 2 cut(s) 971, 1958
BsaBI GATNNNNATC 1 cut(s) 1269
BsaHI GRCGYC 1 cut(s) 51
BsaI GGTCTC 1 cut(s) 2137
BsaJI CCNNGG 9 cut(s) 65, 192, 281, 1571, 1590, 1914, 1968, 2469, 3892
BsaXI ACNNNNNCTCC 4 cut(s) 3632, 3662, 3873, 3903
Bsc4I CCNNNNNNNGG 5 cut(s) 66, 869, 1369, 1761, 2967
Bse1I ACTGG 5 cut(s) 715, 1363, 1440, 2574, 2937
Bse21I CCTNAGG 1 cut(s) 3236
Bse3DI GCAATG 3 cut(s) 1719, 2750, 3135
Bse8I GATNNNNATC 1 cut(s) 1269
BseBI CCWGG 3 cut(s) 1317, 1573, 1969
BseDI CCNNGG 9 cut(s) 65, 192, 281, 1571, 1590, 1914, 1968, 2469, 3892
BseGI GGATG 5 cut(s) 93, 337, 1352, 1359, 2131
BseJI GATNNNNATC 1 cut(s) 1269
BseLI CCNNNNNNNGG 5 cut(s) 66, 869, 1369, 1761, 2967
BseMI GCAATG 3 cut(s) 1719, 2750, 3135
BseNI ACTGG 5 cut(s) 715, 1363, 1440, 2574, 2937
BseRI GAGGAG 6 cut(s) 1061, 1181, 1976, 3095, 3425, 3854
BseSI GKGCMC 2 cut(s) 1915, 3763
BseX3I CGGCCG 1 cut(s) 32
BseXI GCAGC 8 cut(s) 688, 1000, 1366, 3004, 3732, 3901, 3904, 3919
BsgI GTGCAG 1 cut(s) 735
Bsh1236I CGCG 5 cut(s) 194, 201, 306, 850, 1275
Bsh1285I CGRYCG 3 cut(s) 35, 124, 1279
BshFI GGCC 5 cut(s) 34, 1967, 2468, 2960, 3897
BshNI GGYRCC 2 cut(s) 1348, 1751
BsiEI CGRYCG 3 cut(s) 35, 124, 1279
BsiHKAI GWGCWC 8 cut(s) 31, 988, 1519, 1935, 2918, 3377, 3438, 3582
BsiHKCI CYCGRG 1 cut(s) 1914
BsiSI CCGG 4 cut(s) 78, 281, 340, 1915
BslFI GGGAC 5 cut(s) 559, 809, 831, 1778, 3400
BslI CCNNNNNNNGG 5 cut(s) 66, 869, 1369, 1761, 2967
BsmFI GGGAC 5 cut(s) 559, 809, 831, 1778, 3400
BsmI GAATGC 1 cut(s) 2551
BsnI GGCC 5 cut(s) 34, 1967, 2468, 2960, 3897
Bso31I GGTCTC 1 cut(s) 2137
BsoBI CYCGRG 1 cut(s) 1914
Bsp119I TTCGAA 2 cut(s) 114, 2742
Bsp1407I TGTACA 2 cut(s) 561, 2292
Bsp68I TCGCGA 1 cut(s) 1275
BspACI CCGC 9 cut(s) 35, 189, 192, 194, 201, 589, 650, 1279, 3393
BspANI GGCC 5 cut(s) 34, 1967, 2468, 2960, 3897
BspFNI CGCG 5 cut(s) 194, 201, 306, 850, 1275
BspHI TCATGA 2 cut(s) 430, 3931
BspLI GGNNCC 9 cut(s) 214, 405, 575, 742, 1350, 1753, 2060, 2715, 3010
BspMAI CTGCAG 2 cut(s) 2343, 2967
BspMI ACCTGC 1 cut(s) 871
BspPI GGATC 3 cut(s) 1520, 2708, 2721
BspQI GCTCTTC 4 cut(s) 1023, 1524, 1935, 2943
BspT104I TTCGAA 2 cut(s) 114, 2742
BspT107I GGYRCC 2 cut(s) 1348, 1751
BspTI CTTAAG 1 cut(s) 3899
BspTNI GGTCTC 1 cut(s) 2137
BsrBI CCGCTC 1 cut(s) 37
BsrDI GCAATG 3 cut(s) 1719, 2750, 3135
BsrGI TGTACA 2 cut(s) 561, 2292
BsrI ACTGG 5 cut(s) 715, 1363, 1440, 2574, 2937
BssECI CCNNGG 9 cut(s) 65, 192, 281, 1571, 1590, 1914, 1968, 2469, 3892
BssNAI GTATAC 1 cut(s) 2839
BssNI GRCGYC 1 cut(s) 51
BssSI CACGAG 1 cut(s) 1977
BssT1I CCWWGG 3 cut(s) 65, 1590, 2469
Bst1107I GTATAC 1 cut(s) 2839
Bst2BI CACGAG 1 cut(s) 1977
Bst2UI CCWGG 3 cut(s) 1317, 1573, 1969
Bst4CI ACNGT 9 cut(s) 22, 217, 242, 566, 778, 1514, 1775, 2361, 3850
Bst6I CTCTTC 8 cut(s) 1023, 1289, 1524, 1935, 2634, 2817, 2943, 3129
BstACI GRCGYC 1 cut(s) 51
BstAFI CTTAAG 1 cut(s) 3899
BstAPI GCANNNNNTGC 1 cut(s) 1379
BstAUI TGTACA 2 cut(s) 561, 2292
BstBAI YACGTR 2 cut(s) 971, 1958
BstBI TTCGAA 2 cut(s) 114, 2742
BstC8I GCNNGC 7 cut(s) 27, 1617, 1686, 1862, 1890, 2049, 3912
BstDSI CCRYGG 1 cut(s) 192
BstF5I GGATG 5 cut(s) 93, 337, 1352, 1359, 2131
BstFNI CGCG 5 cut(s) 194, 201, 306, 850, 1275
BstHHI GCGC 4 cut(s) 72, 306, 852, 2490
BstMCI CGRYCG 3 cut(s) 35, 124, 1279
BstNI CCWGG 3 cut(s) 1317, 1573, 1969
BstNSI RCATGY 2 cut(s) 824, 1547
BstSCI CCNGG 7 cut(s) 280, 339, 1315, 1571, 1913, 1914, 1967
BstSFI CTRYAG 3 cut(s) 2339, 2963, 3846
BstSLI GKGCMC 2 cut(s) 1915, 3763
BstUI CGCG 5 cut(s) 194, 201, 306, 850, 1275
BstV1I GCAGC 8 cut(s) 688, 1000, 1366, 3004, 3732, 3901, 3904, 3919
BstV2I GAAGAC 7 cut(s) 54, 1485, 1845, 2711, 2859, 3045, 3284
BstX2I RGATCY 3 cut(s) 372, 1609, 2713
BstYI RGATCY 3 cut(s) 372, 1609, 2713
BstZ17I GTATAC 1 cut(s) 2839
BstZI CGGCCG 1 cut(s) 32
Bsu36I CCTNAGG 1 cut(s) 3236
BsuRI GGCC 5 cut(s) 34, 1967, 2468, 2960, 3897
BtgI CCRYGG 1 cut(s) 192
BtgZI GCGATG 2 cut(s) 73, 3062
BtrI CACGTC 2 cut(s) 16, 3390
BtsCI GGATG 5 cut(s) 93, 337, 1352, 1359, 2131
BtsI GCAGTG 1 cut(s) 306
BtsIMutI CAGTG 5 cut(s) 18, 247, 306, 2227, 3024
BtuMI TCGCGA 1 cut(s) 1275
BveI ACCTGC 1 cut(s) 871
Cac8I GCNNGC 7 cut(s) 27, 1617, 1686, 1862, 1890, 2049, 3912
CaiI CAGNNNCTG 1 cut(s) 1013
CciI TCATGA 2 cut(s) 430, 3931
CfoI GCGC 4 cut(s) 72, 306, 852, 2490
Cfr42I CCGCGG 1 cut(s) 195
Cfr9I CCCGGG 1 cut(s) 1914
CpoI CGGWCCG 1 cut(s) 652
CseI GACGC 4 cut(s) 59, 1016, 2864, 3809
Csp6I GTAC 4 cut(s) 562, 1752, 1989, 2293
CspI CGGWCCG 1 cut(s) 652
CviQI GTAC 4 cut(s) 562, 1752, 1989, 2293
DriI GACNNNNNGTC 1 cut(s) 2860
EaeI YGGCCR 2 cut(s) 32, 2466
EagI CGGCCG 1 cut(s) 32
Eam1104I CTCTTC 8 cut(s) 1023, 1289, 1524, 1935, 2634, 2817, 2943, 3129
Eam1105I GACNNNNNGTC 1 cut(s) 2860
EarI CTCTTC 8 cut(s) 1023, 1289, 1524, 1935, 2634, 2817, 2943, 3129
Ecl136II GAGCTC 3 cut(s) 1933, 3375, 3436
EclXI CGGCCG 1 cut(s) 32
Eco130I CCWWGG 3 cut(s) 65, 1590, 2469
Eco24I GRGCYC 3 cut(s) 1935, 3377, 3438
Eco31I GGTCTC 1 cut(s) 2137
Eco52I CGGCCG 1 cut(s) 32
Eco53kI GAGCTC 3 cut(s) 1933, 3375, 3436
Eco57I CTGAAG 5 cut(s) 1418, 2100, 2297, 2561, 3048
Eco72I CACGTG 1 cut(s) 1958
Eco81I CCTNAGG 1 cut(s) 3236
Eco88I CYCGRG 1 cut(s) 1914
EcoICRI GAGCTC 3 cut(s) 1933, 3375, 3436
EcoO109I RGGNCCY 4 cut(s) 573, 3232, 3862, 3889
EcoRI GAATTC 4 cut(s) 9, 116, 344, 2192
EcoRII CCWGG 3 cut(s) 1315, 1571, 1967
EcoT14I CCWWGG 3 cut(s) 65, 1590, 2469
EcoT38I GRGCYC 3 cut(s) 1935, 3377, 3438
ErhI CCWWGG 3 cut(s) 65, 1590, 2469
FaqI GGGAC 5 cut(s) 559, 809, 831, 1778, 3400
FauNDI CATATG 1 cut(s) 226
FbaI TGATCA 2 cut(s) 1981, 3310
FblI GTMKAC 2 cut(s) 2838, 3217
FokI GGATG 5 cut(s) 80, 344, 1339, 1346, 2138
FriOI GRGCYC 3 cut(s) 1935, 3377, 3438
FspBI CTAG 6 cut(s) 440, 683, 2168, 2816, 3560, 3806
FspI TGCGCA 1 cut(s) 2489
GlaI GCGC 4 cut(s) 71, 305, 851, 2489
GsuI CTGGAG 3 cut(s) 474, 1728, 2591
HaeIII GGCC 5 cut(s) 34, 1967, 2468, 2960, 3897
HapII CCGG 4 cut(s) 78, 281, 340, 1915
HgaI GACGC 4 cut(s) 59, 1016, 2864, 3809
HhaI GCGC 4 cut(s) 72, 306, 852, 2490
Hin1I GRCGYC 1 cut(s) 51
Hin6I GCGC 4 cut(s) 70, 304, 850, 2488
HinP1I GCGC 4 cut(s) 70, 304, 850, 2488
HindIII AAGCTT 2 cut(s) 1582, 2937
HpaII CCGG 4 cut(s) 78, 281, 340, 1915
HphI GGTGA 7 cut(s) 146, 298, 635, 2588, 3205, 3338, 3427
Hpy166II GTNNAC 7 cut(s) 337, 679, 1322, 2839, 2865, 3024, 3218
Hpy8I GTNNAC 7 cut(s) 337, 679, 1322, 2839, 2865, 3024, 3218
HpyCH4III ACNGT 9 cut(s) 22, 217, 242, 566, 778, 1514, 1775, 2361, 3850
Hsp92I GRCGYC 1 cut(s) 51
HspAI GCGC 4 cut(s) 70, 304, 850, 2488
KpnI GGTACC 1 cut(s) 1755
Ksp22I TGATCA 2 cut(s) 1981, 3310
KspI CCGCGG 1 cut(s) 195
LguI GCTCTTC 4 cut(s) 1023, 1524, 1935, 2943
LmnI GCTCC 7 cut(s) 409, 1048, 1144, 1709, 3433, 3534, 3744
Lsp1109I GCAGC 8 cut(s) 688, 1000, 1366, 3004, 3732, 3901, 3904, 3919
MaeI CTAG 6 cut(s) 440, 683, 2168, 2816, 3560, 3806
MbiI CCGCTC 1 cut(s) 37
MfeI CAATTG 3 cut(s) 2100, 3543, 3594
MflI RGATCY 3 cut(s) 372, 1609, 2713
MlsI TGGCCA 1 cut(s) 2468
MluNI TGGCCA 1 cut(s) 2468
MmeI TCCRAC 5 cut(s) 1143, 1485, 2358, 3186, 3618
Mox20I TGGCCA 1 cut(s) 2468
MroXI GAANNNNTTC 3 cut(s) 723, 1944, 3281
MscI TGGCCA 1 cut(s) 2468
MslI CAYNNNNRTG 4 cut(s) 621, 927, 993, 3017
Msp20I TGGCCA 1 cut(s) 2468
MspA1I CMGCKG 3 cut(s) 194, 2834, 3269
MspCI CTTAAG 1 cut(s) 3899
MspI CCGG 4 cut(s) 78, 281, 340, 1915
MspR9I CCNGG 7 cut(s) 282, 341, 1317, 1573, 1915, 1916, 1969
MunI CAATTG 3 cut(s) 2100, 3543, 3594
Mva1269I GAATGC 1 cut(s) 2551
MvaI CCWGG 3 cut(s) 1317, 1573, 1969
MvnI CGCG 5 cut(s) 194, 201, 306, 850, 1275
NciI CCSGG 4 cut(s) 282, 341, 1915, 1916
NdeI CATATG 1 cut(s) 226
NlaIV GGNNCC 9 cut(s) 214, 405, 575, 742, 1350, 1753, 2060, 2715, 3010
NmeAIII GCCGAG 4 cut(s) 10, 248, 632, 3873
NmuCI GTSAC 7 cut(s) 16, 973, 1403, 2222, 2639, 2658, 3562
NruI TCGCGA 1 cut(s) 1275
NsbI TGCGCA 1 cut(s) 2489
NspI RCATGY 2 cut(s) 824, 1547
NspV TTCGAA 2 cut(s) 114, 2742
PagI TCATGA 2 cut(s) 430, 3931
PciI ACATGT 2 cut(s) 820, 1543
PciSI GCTCTTC 4 cut(s) 1023, 1524, 1935, 2943
PcsI WCGNNNNNNNCGW 2 cut(s) 1984, 3807
PctI GAATGC 1 cut(s) 2551
PdmI GAANNNNTTC 3 cut(s) 723, 1944, 3281
PflMI CCANNNNNTGG 1 cut(s) 66
Ple19I CGATCG 1 cut(s) 124
PmaCI CACGTG 1 cut(s) 1958
PmlI CACGTG 1 cut(s) 1958
Ppu21I YACGTR 2 cut(s) 971, 1958
PpuMI RGGWCCY 4 cut(s) 573, 3232, 3862, 3889
PscI ACATGT 2 cut(s) 820, 1543
PshBI ATTAAT 3 cut(s) 1758, 2808, 3399
PsiI TTATAA 1 cut(s) 3624
Psp124BI GAGCTC 3 cut(s) 1935, 3377, 3438
Psp1406I AACGTT 1 cut(s) 3619
Psp5II RGGWCCY 4 cut(s) 573, 3232, 3862, 3889
Psp6I CCWGG 3 cut(s) 1315, 1571, 1967
PspCI CACGTG 1 cut(s) 1958
PspGI CCWGG 3 cut(s) 1315, 1571, 1967
PspN4I GGNNCC 9 cut(s) 214, 405, 575, 742, 1350, 1753, 2060, 2715, 3010
PspPPI RGGWCCY 4 cut(s) 573, 3232, 3862, 3889
PstI CTGCAG 2 cut(s) 2343, 2967
PstNI CAGNNNCTG 1 cut(s) 1013
PsuI RGATCY 3 cut(s) 372, 1609, 2713
PvuI CGATCG 1 cut(s) 124
PvuII CAGCTG 2 cut(s) 2834, 3269
RruI TCGCGA 1 cut(s) 1275
RsaI GTAC 4 cut(s) 563, 1753, 1990, 2294
RsaNI GTAC 4 cut(s) 562, 1752, 1989, 2293
RseI CAYNNNNRTG 4 cut(s) 621, 927, 993, 3017
Rsr2I CGGWCCG 1 cut(s) 652
RsrII CGGWCCG 1 cut(s) 652
SacI GAGCTC 3 cut(s) 1935, 3377, 3438
SacII CCGCGG 1 cut(s) 195
SapI GCTCTTC 4 cut(s) 1023, 1524, 1935, 2943
ScrFI CCNGG 7 cut(s) 282, 341, 1317, 1573, 1915, 1916, 1969
SfcI CTRYAG 3 cut(s) 2339, 2963, 3846
Sfr303I CCGCGG 1 cut(s) 195
SfuI TTCGAA 2 cut(s) 114, 2742
SgrBI CCGCGG 1 cut(s) 195
SmaI CCCGGG 1 cut(s) 1916
SmiMI CAYNNNNRTG 4 cut(s) 621, 927, 993, 3017
SmlI CTYRAG 4 cut(s) 1022, 2270, 3360, 3899
SmoI CTYRAG 4 cut(s) 1022, 2270, 3360, 3899
SsiI CCGC 9 cut(s) 35, 189, 192, 194, 201, 589, 650, 1279, 3393
SspI AATATT 1 cut(s) 2073
SspMI CTAG 6 cut(s) 440, 683, 2168, 2816, 3560, 3806
SstI GAGCTC 3 cut(s) 1935, 3377, 3438
StyD4I CCNGG 7 cut(s) 280, 339, 1315, 1571, 1913, 1914, 1967
StyI CCWWGG 3 cut(s) 65, 1590, 2469
TaaI ACNGT 9 cut(s) 22, 217, 242, 566, 778, 1514, 1775, 2361, 3850
TatI WGTACW 2 cut(s) 561, 2292
TauI GCSGC 4 cut(s) 37, 191, 194, 204
TscAI CASTG 5 cut(s) 25, 247, 306, 2227, 3031
TseFI GTSAC 7 cut(s) 16, 973, 1403, 2222, 2639, 2658, 3562
TseI GCWGC 8 cut(s) 701, 1013, 1379, 2992, 3720, 3907, 3914, 3917
Tsp45I GTSAC 7 cut(s) 16, 973, 1403, 2222, 2639, 2658, 3562
TspGWI ACGGA 3 cut(s) 644, 1693, 2810
TspMI CCCGGG 1 cut(s) 1914
TspRI CASTG 5 cut(s) 25, 247, 306, 2227, 3031
Van91I CCANNNNNTGG 1 cut(s) 66
Vha464I CTTAAG 1 cut(s) 3899
VspI ATTAAT 3 cut(s) 1758, 2808, 3399
XapI RAATTY 9 cut(s) 9, 116, 126, 344, 426, 670, 918, 2192, 2350
XbaI TCTAGA 3 cut(s) 439, 2815, 3805
XceI RCATGY 2 cut(s) 824, 1547
XcmI CCANNNNNNNNNTGG 1 cut(s) 3127
XmaI CCCGGG 1 cut(s) 1914
XmiI GTMKAC 2 cut(s) 2838, 3217
XmnI GAANNNNTTC 3 cut(s) 723, 1944, 3281
XspI CTAG 6 cut(s) 440, 683, 2168, 2816, 3560, 3806
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.