MD12G1098900.v1.1

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0006414
Physical Location & Seq
Forward (+)
15456350 .. 15456847
498 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1098900.v1.1.491

Sequence Viewer

Length: 498 bp
ATGCCACACAGGACGCTCGCTGAGCTAAAACACAAGTTTGGTCCTGTAATTTGGCTAAGAGTTGGTGCAAGAAACACCATGGTAATTCAATCTGCTAAAGCTGCCGCCAACCTTTTCAAGAACCATGACATCTCATTAGCAGGACGCCCCACGACTGAAGCAAGCCAAGTTTATGACTACCACAAAGGTTCATTAGCCCTAGCCCCTTACGGCTCACACTGGCGTGTGTTAAGGCGGTTAGTCACGGTTGACATGCTGGTGAACAAGCGCATCAATGAGACGGGTTTCGTAAGGAAAAAGTGCCTTGAAATATTGCAATTGTGGATAGAAGAGGAGGCGTCAAAACTGAAAGAAGGGCACGGAGTTCATGTGGCAAGGTTTGTGTTCCTTATGAGCTTCAACCTTCTTGGCAACCTCATGTTGTCTAAGGACTTGGTGGACCCGAATTCGAAAGAAGGGTCCGAGTTTTTTACAGCAATGACTGGAATGATGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

166

Amino Acids

18.7

Weight (kDa)

9.59

Isoelectric Point (pI)

43.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 1 - 160 1.5e-15 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 105, 235
AcsI RAATTY 1 cut(s) 445
AcuI CTGAAG 1 cut(s) 177
AcyI GRCGYC 2 cut(s) 145, 338
AgsI TTSAA 4 cut(s) 89, 118, 308, 400
AleI CACNNNNGTG 1 cut(s) 222
AluBI AGCT 3 cut(s) 25, 101, 396
AluI AGCT 3 cut(s) 25, 101, 396
Alw26I GTCTC 1 cut(s) 272
ApeKI GCWGC 1 cut(s) 101
ApoI RAATTY 1 cut(s) 445
ArsI GACNNNNNNTTYG 2 cut(s) 443, 475
AspLEI GCGC 1 cut(s) 270
AspS9I GGNCC 3 cut(s) 41, 439, 459
AsuHPI GGTGA 1 cut(s) 271
AsuII TTCGAA 1 cut(s) 449
AvaII GGWCC 3 cut(s) 41, 439, 459
BaeGI GKGCMC 1 cut(s) 360
BbvI GCAGC 1 cut(s) 88
BccI CCATC 1 cut(s) 484
BceAI ACGGC 1 cut(s) 226
BcoDI GTCTC 1 cut(s) 272
BfaI CTAG 1 cut(s) 200
BisI GCNGC 2 cut(s) 102, 105
BlpI GCTNAGC 1 cut(s) 21
BlsI GCNGC 2 cut(s) 103, 106
Bme18I GGWCC 3 cut(s) 41, 439, 459
BmgT120I GGNCC 3 cut(s) 41, 439, 459
BmiI GGNNCC 2 cut(s) 441, 460
BmsI GCATC 1 cut(s) 279
Bpu1102I GCTNAGC 1 cut(s) 21
Bpu14I TTCGAA 1 cut(s) 449
BsaHI GRCGYC 2 cut(s) 145, 338
BsaJI CCNNGG 1 cut(s) 78
BsaXI ACNNNNNCTCC 2 cut(s) 354, 384
Bse1I ACTGG 2 cut(s) 224, 487
Bse3DI GCAATG 1 cut(s) 483
BseDI CCNNGG 1 cut(s) 78
BseMI GCAATG 1 cut(s) 483
BseMII CTCAG 1 cut(s) 12
BseNI ACTGG 2 cut(s) 224, 487
BseRI GAGGAG 1 cut(s) 347
BseSI GKGCMC 1 cut(s) 360
BseXI GCAGC 1 cut(s) 88
BsmAI GTCTC 1 cut(s) 272
BsmBI CGTCTC 1 cut(s) 272
Bsp119I TTCGAA 1 cut(s) 449
Bsp1286I GDGCHC 1 cut(s) 360
Bsp1720I GCTNAGC 1 cut(s) 21
Bsp19I CCATGG 1 cut(s) 78
BspACI CCGC 2 cut(s) 105, 235
BspCNI CTCAG 1 cut(s) 13
BspLI GGNNCC 2 cut(s) 441, 460
BspT104I TTCGAA 1 cut(s) 449
BsrDI GCAATG 1 cut(s) 483
BsrI ACTGG 2 cut(s) 224, 487
BssECI CCNNGG 1 cut(s) 78
BssNI GRCGYC 2 cut(s) 145, 338
BssT1I CCWWGG 1 cut(s) 78
Bst4CI ACNGT 1 cut(s) 247
Bst6I CTCTTC 1 cut(s) 324
BstACI GRCGYC 2 cut(s) 145, 338
BstBI TTCGAA 1 cut(s) 449
BstC8I GCNNGC 2 cut(s) 18, 163
BstDEI CTNAG 3 cut(s) 21, 56, 426
BstDSI CCRYGG 1 cut(s) 78
BstHHI GCGC 1 cut(s) 270
BstMAI GTCTC 1 cut(s) 272
BstMWI GCNNNNNNNGC 2 cut(s) 22, 101
BstNSI RCATGY 1 cut(s) 256
BstSLI GKGCMC 1 cut(s) 360
BstV1I GCAGC 1 cut(s) 88
BtgI CCRYGG 1 cut(s) 78
BtsIMutI CAGTG 1 cut(s) 217
Cac8I GCNNGC 2 cut(s) 18, 163
CfoI GCGC 1 cut(s) 270
Cfr13I GGNCC 3 cut(s) 41, 439, 459
CseI GACGC 3 cut(s) 22, 153, 327
CviAII CATG 5 cut(s) 79, 125, 253, 368, 418
CviJI RGCY 8 cut(s) 25, 55, 101, 165, 197, 203, 213, 396
CviKI_1 RGCY 8 cut(s) 25, 55, 101, 165, 197, 203, 213, 396
DdeI CTNAG 3 cut(s) 21, 56, 426
Eam1104I CTCTTC 1 cut(s) 324
EarI CTCTTC 1 cut(s) 324
Eco130I CCWWGG 1 cut(s) 78
Eco47I GGWCC 3 cut(s) 41, 439, 459
Eco57I CTGAAG 1 cut(s) 177
EcoRI GAATTC 1 cut(s) 445
EcoT14I CCWWGG 1 cut(s) 78
ErhI CCWWGG 1 cut(s) 78
Esp3I CGTCTC 1 cut(s) 272
FaeI CATG 5 cut(s) 82, 128, 256, 371, 421
FaiI YATR 7 cut(s) 80, 126, 174, 254, 369, 392, 419
FatI CATG 5 cut(s) 78, 124, 252, 367, 417
Fnu4HI GCNGC 2 cut(s) 102, 105
Fsp4HI GCNGC 2 cut(s) 102, 105
FspBI CTAG 1 cut(s) 200
GlaI GCGC 1 cut(s) 269
GluI GCNGC 2 cut(s) 102, 105
HgaI GACGC 3 cut(s) 22, 153, 327
HhaI GCGC 1 cut(s) 270
Hin1I GRCGYC 2 cut(s) 145, 338
Hin1II CATG 5 cut(s) 82, 128, 256, 371, 421
Hin6I GCGC 1 cut(s) 268
HinP1I GCGC 1 cut(s) 268
HincII GTYRAC 1 cut(s) 250
HindII GTYRAC 1 cut(s) 250
HphI GGTGA 1 cut(s) 271
Hpy166II GTNNAC 3 cut(s) 250, 262, 439
Hpy188I TCNGA 1 cut(s) 463
Hpy188III TCNNGA 1 cut(s) 118
Hpy8I GTNNAC 3 cut(s) 250, 262, 439
HpyAV CCTTC 3 cut(s) 347, 413, 449
HpyCH4III ACNGT 1 cut(s) 247
HpyCH4V TGCA 2 cut(s) 68, 316
HpyF10VI GCNNNNNNNGC 2 cut(s) 22, 101
HpyF3I CTNAG 3 cut(s) 21, 56, 426
Hsp92I GRCGYC 2 cut(s) 145, 338
Hsp92II CATG 5 cut(s) 82, 128, 256, 371, 421
HspAI GCGC 1 cut(s) 268
LpnPI CCDG 5 cut(s) 57, 126, 205, 242, 468
Lsp1109I GCAGC 1 cut(s) 88
LweI GCATC 1 cut(s) 279
MaeI CTAG 1 cut(s) 200
MaeIII GTNAC 1 cut(s) 241
MboII GAAGA 1 cut(s) 341
MfeI CAATTG 1 cut(s) 317
MhlI GDGCHC 1 cut(s) 360
MluCI AATT 4 cut(s) 48, 84, 317, 445
MnlI CCTC 3 cut(s) 325, 328, 425
MseI TTAA 1 cut(s) 230
MslI CAYNNNNRTG 2 cut(s) 222, 257
MunI CAATTG 1 cut(s) 317
MwoI GCNNNNNNNGC 2 cut(s) 22, 101
NcoI CCATGG 1 cut(s) 78
NlaIII CATG 5 cut(s) 82, 128, 256, 371, 421
NlaIV GGNNCC 2 cut(s) 441, 460
NmuCI GTSAC 1 cut(s) 241
NspI RCATGY 1 cut(s) 256
NspV TTCGAA 1 cut(s) 449
OliI CACNNNNGTG 1 cut(s) 222
PkrI GCNGC 2 cut(s) 103, 106
PspN4I GGNNCC 2 cut(s) 441, 460
PspPI GGNCC 3 cut(s) 41, 439, 459
RseI CAYNNNNRTG 2 cut(s) 222, 257
SaqAI TTAA 1 cut(s) 230
SatI GCNGC 2 cut(s) 102, 105
Sau96I GGNCC 3 cut(s) 41, 439, 459
SduI GDGCHC 1 cut(s) 360
SetI ASST 8 cut(s) 27, 103, 114, 190, 380, 398, 405, 417
SfaNI GCATC 1 cut(s) 279
SfuI TTCGAA 1 cut(s) 449
SinI GGWCC 3 cut(s) 41, 439, 459
SmiMI CAYNNNNRTG 2 cut(s) 222, 257
Sse9I AATT 4 cut(s) 48, 84, 317, 445
SsiI CCGC 2 cut(s) 105, 235
SspI AATATT 1 cut(s) 312
SspMI CTAG 1 cut(s) 200
StyI CCWWGG 1 cut(s) 78
TaaI ACNGT 1 cut(s) 247
TaqI TCGA 1 cut(s) 449
TasI AATT 4 cut(s) 48, 84, 317, 445
TauI GCSGC 1 cut(s) 107
Tru1I TTAA 1 cut(s) 230
Tru9I TTAA 1 cut(s) 230
TscAI CASTG 1 cut(s) 224
TseFI GTSAC 1 cut(s) 241
TseI GCWGC 1 cut(s) 101
Tsp45I GTSAC 1 cut(s) 241
TspDTI ATGAA 2 cut(s) 180, 356
TspGWI ACGGA 1 cut(s) 375
TspRI CASTG 1 cut(s) 224
VpaK11BI GGWCC 3 cut(s) 41, 439, 459
XapI RAATTY 1 cut(s) 445
XceI RCATGY 1 cut(s) 256
XspI CTAG 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.