Rh3BG196900

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0006414
Physical Location & Seq
Forward (+)
17459726 .. 17465318
5593 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG196900.1

Sequence Viewer

Length: 1104 bp
ATGGAGTACTTTCCCTCGAATTTTCTTGTCTGTTTTGTCATACCATCTCTGCTCTTCTTGCTCTTCCGGTGGAGATCAAATTCAGGTGGTAATCAAGGGCTTCCACCCGGCCCACCGGGATGGCCACTTCTCGGCAACATGCTCGACCTTGGAGCTATGCCGCACAAAACCCTCACCAGGCTGCGACACAAATTCGGTTCTGTGGTCTGGCTAAGGATCGGTGTCCGAAATACCTTGGTAATCCAATCCACCAAAGCAGCCACCGAGTTTTTCAAAAACCACGACCTGACGTTTGTTGAACGGAATATCAATGAAGGTGCTCGTGTTCACGATTACCATAAGGGGTCACTCGCGCTGGCCCCTTACGGGACACAGTGGCGAGTGTTAAGGCGTCTAGTGACCGTGGACATGTTGACGAACAAGCGTATCAATGAGACGGCAATGATACGCAGAAAGTGCGTTGAAAATATGCAAAAATGGATAGAAGAAGAAGCCTCTAAACTCAACGATAATGGATCATCGTCAAATGGAGTGCACGTCGCTCGTTTCGTGTTCCTCATGACATTTAATCTTCTTGGGAATCTAATGTTGTCGAGGGACTTGGTGGATCCAAATTCAAAAGAAGGATTGGAGTTTTTTGACGCGATGAGGGGACTGATGGAGGCGAATGGTCATGCAAACATGGCGGACTACTTTCCATGGCTGAGGTGGCTTGATCCACAGGGTGTGAAGAGAAGGATGAAGAAGCACGTTGGAAAAGCTCTGGAAATTGCCTCCGGCTTTGTGAAGGCGCGCGTGAAAGAGAGGAAATTGGTGGGTGGAGAGAAAACTAGGGACTTTCTGGATGTGATGCTTGACTTTGAAGACGATGGAAATCATGAATCAGCCAAGATTTCTGATCATCATCTCAATATTTTCATTCTGTCTGACGTGTTTCAGAATCCAGGTCGGATAGTTTGCTGGGTTCAGGTTCAACCTTTTGAGTGTCTATTTCGGGATCACCTTTCACTAAAGACTTCCACCAGTCCTGTTGGTTCTTCTTGGTCAAAAGAATGGAGACAGCAGTCTGATCCTCTATGCTCCACAAACAATCATCAGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

367

Amino Acids

42.0

Weight (kDa)

9.34

Isoelectric Point (pI)

43.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 35 - 307 5.1e-32 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 353, 644, 793, 795
AciI CCGC 2 cut(s) 161, 686
AclWI GGATC 7 cut(s) 224, 523, 602, 615, 710, 1005, 1064
AcoI YGGCCR 1 cut(s) 122
AcsI RAATTY 4 cut(s) 19, 79, 191, 613
AcyI GRCGYC 1 cut(s) 391
AdeI CACNNNGTG 1 cut(s) 725
AfaI GTAC 1 cut(s) 8
AfiI CCNNNNNNNGG 3 cut(s) 131, 177, 366
AflIII ACRYGT 2 cut(s) 408, 930
AgsI TTSAA 6 cut(s) 274, 299, 464, 618, 863, 974
AjiI CACGTC 2 cut(s) 538, 931
AjnI CCWGG 2 cut(s) 176, 943
AluBI AGCT 2 cut(s) 155, 761
AluI AGCT 2 cut(s) 155, 761
Alw21I GWGCWC 2 cut(s) 322, 537
Alw26I GTCTC 2 cut(s) 428, 1051
Alw44I GTGCAC 1 cut(s) 533
AlwI GGATC 7 cut(s) 224, 523, 602, 615, 710, 1005, 1064
AoxI GGCC 3 cut(s) 109, 122, 357
ApaLI GTGCAC 1 cut(s) 533
ApeKI GCWGC 2 cut(s) 181, 257
ApoI RAATTY 4 cut(s) 19, 79, 191, 613
ArsI GACNNNNNNTTYG 2 cut(s) 275, 307
AspLEI GCGC 3 cut(s) 355, 793, 795
AspS9I GGNCC 2 cut(s) 110, 358
AsuC2I CCSGG 2 cut(s) 108, 117
AsuHPI GGTGA 2 cut(s) 166, 992
BaeGI GKGCMC 1 cut(s) 537
BaeI ACNNNNGTAYC 2 cut(s) 409, 442
BalI TGGCCA 1 cut(s) 124
BamHI GGATCC 1 cut(s) 607
BauI CACGAG 1 cut(s) 321
BbsI GAAGAC 1 cut(s) 870
Bbv12I GWGCWC 2 cut(s) 322, 537
BbvCI CCTCAGC 1 cut(s) 704
BbvI GCAGC 2 cut(s) 168, 269
BccI CCATC 4 cut(s) 52, 114, 652, 863
BceAI ACGGC 1 cut(s) 453
BcgI CGANNNNNNTGC 4 cut(s) 124, 158, 524, 558
BciT130I CCWGG 2 cut(s) 178, 945
BclI TGATCA 1 cut(s) 898
BcnI CCSGG 2 cut(s) 108, 117
BcoDI GTCTC 2 cut(s) 428, 1051
BfaI CTAG 2 cut(s) 395, 831
BisI GCNGC 3 cut(s) 161, 182, 258
BlsI GCNGC 3 cut(s) 162, 183, 259
BmcAI AGTACT 1 cut(s) 8
Bme1390I CCNGG 4 cut(s) 108, 117, 178, 945
BmgBI CACGTC 2 cut(s) 538, 931
BmgT120I GGNCC 2 cut(s) 110, 358
BmiI GGNNCC 2 cut(s) 360, 609
BmrFI CCNGG 4 cut(s) 108, 117, 178, 945
BmsI GCATC 1 cut(s) 840
BoxI GACNNNNGTC 1 cut(s) 1063
BpiI GAAGAC 1 cut(s) 870
Bpu10I CCTNAGC 2 cut(s) 212, 704
BpuMI CCSGG 2 cut(s) 108, 117
BsaBI GATNNNNATC 2 cut(s) 873, 903
BsaHI GRCGYC 1 cut(s) 391
BsaJI CCNNGG 4 cut(s) 148, 234, 402, 698
BsaWI WCCGGW 1 cut(s) 66
BsaXI ACNNNNNCTCC 2 cut(s) 522, 552
Bsc4I CCNNNNNNNGG 3 cut(s) 131, 177, 366
Bse1I ACTGG 1 cut(s) 1023
Bse3DI GCAATG 1 cut(s) 447
Bse8I GATNNNNATC 2 cut(s) 873, 903
BseBI CCWGG 2 cut(s) 178, 945
BseDI CCNNGG 4 cut(s) 148, 234, 402, 698
BseGI GGATG 3 cut(s) 125, 744, 850
BseJI GATNNNNATC 2 cut(s) 873, 903
BseLI CCNNNNNNNGG 3 cut(s) 131, 177, 366
BseMI GCAATG 1 cut(s) 447
BseMII CTCAG 1 cut(s) 695
BseNI ACTGG 1 cut(s) 1023
BsePI GCGCGC 1 cut(s) 791
BseSI GKGCMC 1 cut(s) 537
BseXI GCAGC 2 cut(s) 168, 269
BseYI CCCAGC 1 cut(s) 960
Bsh1236I CGCG 4 cut(s) 353, 644, 793, 795
BshFI GGCC 3 cut(s) 111, 124, 359
BsiHKAI GWGCWC 2 cut(s) 322, 537
BsiSI CCGG 4 cut(s) 67, 108, 116, 777
BslFI GGGAC 4 cut(s) 382, 611, 666, 848
BslI CCNNNNNNNGG 3 cut(s) 131, 177, 366
BsmAI GTCTC 2 cut(s) 428, 1051
BsmBI CGTCTC 1 cut(s) 428
BsmFI GGGAC 4 cut(s) 382, 611, 666, 848
BsnI GGCC 3 cut(s) 111, 124, 359
Bsp1286I GDGCHC 2 cut(s) 322, 537
Bsp143I GATC 8 cut(s) 74, 216, 515, 607, 715, 898, 997, 1069
Bsp19I CCATGG 1 cut(s) 698
BspACI CCGC 2 cut(s) 161, 686
BspANI GGCC 3 cut(s) 111, 124, 359
BspCNI CTCAG 1 cut(s) 696
BspFNI CGCG 4 cut(s) 353, 644, 793, 795
BspHI TCATGA 2 cut(s) 558, 877
BspLI GGNNCC 2 cut(s) 360, 609
BspPI GGATC 7 cut(s) 224, 523, 602, 615, 710, 1005, 1064
BspQI GCTCTTC 2 cut(s) 59, 68
BsrDI GCAATG 1 cut(s) 447
BsrI ACTGG 1 cut(s) 1023
BssECI CCNNGG 4 cut(s) 148, 234, 402, 698
BssHII GCGCGC 1 cut(s) 791
BssMI GATC 8 cut(s) 74, 216, 515, 607, 715, 898, 997, 1069
BssNI GRCGYC 1 cut(s) 391
BssSI CACGAG 1 cut(s) 321
BssT1I CCWWGG 3 cut(s) 148, 234, 698
Bst2BI CACGAG 1 cut(s) 321
Bst2UI CCWGG 2 cut(s) 178, 945
Bst4CI ACNGT 2 cut(s) 375, 403
Bst6I CTCTTC 3 cut(s) 59, 68, 725
BstACI GRCGYC 1 cut(s) 391
BstAPI GCANNNNNTGC 1 cut(s) 456
BstC8I GCNNGC 2 cut(s) 357, 793
BstDEI CTNAG 2 cut(s) 212, 704
BstDSI CCRYGG 2 cut(s) 402, 698
BstF5I GGATG 3 cut(s) 125, 744, 850
BstFNI CGCG 4 cut(s) 353, 644, 793, 795
BstHHI GCGC 3 cut(s) 355, 793, 795
BstKTI GATC 8 cut(s) 77, 219, 518, 610, 718, 901, 1000, 1072
BstMAI GTCTC 2 cut(s) 428, 1051
BstMBI GATC 8 cut(s) 74, 216, 515, 607, 715, 898, 997, 1069
BstMWI GCNNNNNNNGC 4 cut(s) 58, 456, 683, 709
BstNI CCWGG 2 cut(s) 178, 945
BstNSI RCATGY 2 cut(s) 142, 412
BstPAI GACNNNNGTC 1 cut(s) 1063
BstSCI CCNGG 4 cut(s) 106, 115, 176, 943
BstSLI GKGCMC 1 cut(s) 537
BstUI CGCG 4 cut(s) 353, 644, 793, 795
BstV1I GCAGC 2 cut(s) 168, 269
BstV2I GAAGAC 1 cut(s) 870
BstX2I RGATCY 1 cut(s) 607
BstXI CCANNNNNNTGG 1 cut(s) 120
BstYI RGATCY 1 cut(s) 607
BsuRI GGCC 3 cut(s) 111, 124, 359
BtgI CCRYGG 2 cut(s) 402, 698
BtgZI GCGATG 1 cut(s) 659
BtrI CACGTC 2 cut(s) 538, 931
BtsCI GGATG 3 cut(s) 125, 744, 850
BtsIMutI CAGTG 1 cut(s) 380
Cac8I GCNNGC 2 cut(s) 357, 793
CciI TCATGA 2 cut(s) 558, 877
CfoI GCGC 3 cut(s) 355, 793, 795
Cfr13I GGNCC 2 cut(s) 110, 358
CseI GACGC 2 cut(s) 380, 650
Csp6I GTAC 1 cut(s) 7
CviAII CATG 7 cut(s) 139, 409, 559, 674, 682, 699, 878
CviQI GTAC 1 cut(s) 7
DdeI CTNAG 2 cut(s) 212, 704
DpnI GATC 8 cut(s) 76, 218, 517, 609, 717, 900, 999, 1071
DpnII GATC 8 cut(s) 74, 216, 515, 607, 715, 898, 997, 1069
DraIII CACNNNGTG 1 cut(s) 725
EaeI YGGCCR 1 cut(s) 122
Eam1104I CTCTTC 3 cut(s) 59, 68, 725
EarI CTCTTC 3 cut(s) 59, 68, 725
EciI GGCGGA 1 cut(s) 701
Eco130I CCWWGG 3 cut(s) 148, 234, 698
EcoRII CCWGG 2 cut(s) 176, 943
EcoT14I CCWWGG 3 cut(s) 148, 234, 698
ErhI CCWWGG 3 cut(s) 148, 234, 698
Esp3I CGTCTC 1 cut(s) 428
FaeI CATG 7 cut(s) 142, 412, 562, 677, 685, 702, 881
FaqI GGGAC 4 cut(s) 382, 611, 666, 848
FatI CATG 7 cut(s) 138, 408, 558, 673, 681, 698, 877
FbaI TGATCA 1 cut(s) 898
Fnu4HI GCNGC 3 cut(s) 161, 182, 258
FokI GGATG 3 cut(s) 132, 751, 857
Fsp4HI GCNGC 3 cut(s) 161, 182, 258
FspBI CTAG 2 cut(s) 395, 831
GlaI GCGC 3 cut(s) 354, 792, 794
GluI GCNGC 3 cut(s) 161, 182, 258
GsaI CCCAGC 1 cut(s) 964
HaeIII GGCC 3 cut(s) 111, 124, 359
HapII CCGG 4 cut(s) 67, 108, 116, 777
HgaI GACGC 2 cut(s) 380, 650
HhaI GCGC 3 cut(s) 355, 793, 795
Hin1I GRCGYC 1 cut(s) 391
Hin1II CATG 7 cut(s) 142, 412, 562, 677, 685, 702, 881
Hin6I GCGC 3 cut(s) 353, 791, 793
HinP1I GCGC 3 cut(s) 353, 791, 793
HincII GTYRAC 1 cut(s) 414
HindII GTYRAC 1 cut(s) 414
HinfI GANTC 3 cut(s) 580, 881, 940
HpaII CCGG 4 cut(s) 67, 108, 116, 777
HphI GGTGA 2 cut(s) 166, 992
Hpy166II GTNNAC 4 cut(s) 328, 406, 414, 535
Hpy188I TCNGA 6 cut(s) 227, 898, 928, 939, 951, 1069
Hpy188III TCNNGA 6 cut(s) 329, 559, 764, 842, 878, 995
Hpy8I GTNNAC 4 cut(s) 328, 406, 414, 535
Hpy99I CGWCG 1 cut(s) 542
HpyAV CCTTC 4 cut(s) 308, 617, 729, 781
HpyCH4III ACNGT 2 cut(s) 375, 403
HpyCH4IV ACGT 4 cut(s) 290, 537, 750, 930
HpyCH4V TGCA 3 cut(s) 472, 535, 677
HpyF10VI GCNNNNNNNGC 4 cut(s) 58, 456, 683, 709
HpyF3I CTNAG 2 cut(s) 212, 704
HpySE526I ACGT 4 cut(s) 290, 537, 750, 930
Hsp92I GRCGYC 1 cut(s) 391
Hsp92II CATG 7 cut(s) 142, 412, 562, 677, 685, 702, 881
HspAI GCGC 3 cut(s) 353, 791, 793
Ksp22I TGATCA 1 cut(s) 898
Kzo9I GATC 8 cut(s) 74, 216, 515, 607, 715, 898, 997, 1069
LguI GCTCTTC 2 cut(s) 59, 68
LmnI GCTCC 2 cut(s) 152, 1085
Lsp1109I GCAGC 2 cut(s) 168, 269
LweI GCATC 1 cut(s) 840
MaeI CTAG 2 cut(s) 395, 831
MaeII ACGT 4 cut(s) 290, 537, 750, 930
MaeIII GTNAC 2 cut(s) 345, 397
MalI GATC 8 cut(s) 76, 218, 517, 609, 717, 900, 999, 1071
MboI GATC 8 cut(s) 74, 216, 515, 607, 715, 898, 997, 1069
MboII GAAGA 9 cut(s) 46, 55, 497, 500, 563, 742, 754, 875, 1029
MflI RGATCY 1 cut(s) 607
MhlI GDGCHC 2 cut(s) 322, 537
MlsI TGGCCA 1 cut(s) 124
MluCI AATT 6 cut(s) 19, 79, 191, 613, 768, 809
MluNI TGGCCA 1 cut(s) 124
MmeI TCCRAC 2 cut(s) 733, 929
Mox20I TGGCCA 1 cut(s) 124
MscI TGGCCA 1 cut(s) 124
MseI TTAA 2 cut(s) 386, 567
MslI CAYNNNNRTG 1 cut(s) 118
Msp20I TGGCCA 1 cut(s) 124
MspI CCGG 4 cut(s) 67, 108, 116, 777
MspR9I CCNGG 4 cut(s) 108, 117, 178, 945
MvaI CCWGG 2 cut(s) 178, 945
MvnI CGCG 4 cut(s) 353, 644, 793, 795
MwoI GCNNNNNNNGC 4 cut(s) 58, 456, 683, 709
NciI CCSGG 2 cut(s) 108, 117
NcoI CCATGG 1 cut(s) 698
NdeII GATC 8 cut(s) 74, 216, 515, 607, 715, 898, 997, 1069
NlaIII CATG 7 cut(s) 142, 412, 562, 677, 685, 702, 881
NlaIV GGNNCC 2 cut(s) 360, 609
NmeAIII GCCGAG 1 cut(s) 111
NmuCI GTSAC 2 cut(s) 345, 397
NspI RCATGY 2 cut(s) 142, 412
PagI TCATGA 2 cut(s) 558, 877
PauI GCGCGC 1 cut(s) 791
PciI ACATGT 1 cut(s) 408
PciSI GCTCTTC 2 cut(s) 59, 68
PcsI WCGNNNNNNNCGW 1 cut(s) 546
PfeI GAWTC 3 cut(s) 580, 881, 940
PkrI GCNGC 3 cut(s) 162, 183, 259
PscI ACATGT 1 cut(s) 408
PshAI GACNNNNGTC 1 cut(s) 1063
Psp6I CCWGG 2 cut(s) 176, 943
PspFI CCCAGC 1 cut(s) 960
PspGI CCWGG 2 cut(s) 176, 943
PspN4I GGNNCC 2 cut(s) 360, 609
PspPI GGNCC 2 cut(s) 110, 358
PsuI RGATCY 1 cut(s) 607
PteI GCGCGC 1 cut(s) 791
RsaI GTAC 1 cut(s) 8
RsaNI GTAC 1 cut(s) 7
RseI CAYNNNNRTG 1 cut(s) 118
SapI GCTCTTC 2 cut(s) 59, 68
SaqAI TTAA 2 cut(s) 386, 567
SatI GCNGC 3 cut(s) 161, 182, 258
Sau3AI GATC 8 cut(s) 74, 216, 515, 607, 715, 898, 997, 1069
Sau96I GGNCC 2 cut(s) 110, 358
ScaI AGTACT 1 cut(s) 8
ScrFI CCNGG 4 cut(s) 108, 117, 178, 945
SduI GDGCHC 2 cut(s) 322, 537
SfaNI GCATC 1 cut(s) 840
SmiMI CAYNNNNRTG 1 cut(s) 118
Sse9I AATT 6 cut(s) 19, 79, 191, 613, 768, 809
SsiI CCGC 2 cut(s) 161, 686
SspI AATATT 1 cut(s) 913
SspMI CTAG 2 cut(s) 395, 831
StyD4I CCNGG 4 cut(s) 106, 115, 176, 943
StyI CCWWGG 3 cut(s) 148, 234, 698
TaaI ACNGT 2 cut(s) 375, 403
TaiI ACGT 4 cut(s) 293, 540, 753, 933
TaqI TCGA 3 cut(s) 17, 144, 593
TasI AATT 6 cut(s) 19, 79, 191, 613, 768, 809
TatI WGTACW 1 cut(s) 6
TauI GCSGC 1 cut(s) 163
TfiI GAWTC 3 cut(s) 580, 881, 940
Tru1I TTAA 2 cut(s) 386, 567
Tru9I TTAA 2 cut(s) 386, 567
TscAI CASTG 1 cut(s) 380
TseFI GTSAC 2 cut(s) 345, 397
TseI GCWGC 2 cut(s) 181, 257
Tsp45I GTSAC 2 cut(s) 345, 397
TspDTI ATGAA 4 cut(s) 327, 755, 894, 907
TspGWI ACGGA 1 cut(s) 316
TspRI CASTG 1 cut(s) 380
VneI GTGCAC 1 cut(s) 533
XapI RAATTY 4 cut(s) 19, 79, 191, 613
XceI RCATGY 2 cut(s) 142, 412
XcmI CCANNNNNNNNNTGG 1 cut(s) 705
XspI CTAG 2 cut(s) 395, 831
ZrmI AGTACT 1 cut(s) 8
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.