Rroxscaffold_6G00409520

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0006414
Physical Location & Seq
Reverse (-)
32027186 .. 32029371
2186 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00409520.1

Sequence Viewer

Length: 1512 bp
ATGCCTCACAAAACACTCACTCATCTAAGCCGCAAGTACGGTCCGGTAATGTGGCTTAGACTGGGTGCCAAAAACACGATGGTAATACAGTCTACCAAAGCGGCCACCATGTTTTTCAAGCAACACGACCTGTCGTTCGTGGACCGCACCGTCTATGAAGTAAGCCGTGTGTATGGCTACCACAAGGGGTCGCTGGCTTTATCCCCTTACGGATCTCACTGGCGGGTTATGAGACGCCTACTCACGATGCACATGTTGGTCAACCACCGCATCAACGACACGGCGTCTATTCGTCGGAAGTGCGCCGACGACTTGCAGTCGTGGATCGAAGAGGAAGCGTCGTCGTCGTCATCTCATGGAGTGGAGGTGGCACGTTTGGTGTTCCTCATGACGTTCAACGTTTTCGGGAACCTGGTGTTGTCGAGGGACTTGGTGGGACCCAAATCGGAGGAAGGGATGGAGTTTTTCCGGGCGGTGCATGGATTGATGGAGTGGAGTGGGCATGCAAACATGGCCGATTACTTTCCGTGGCTGAAGTGGCTTGACCCGCAAGGTTTGAAGAGGAAGATGAAGAGGGATCTTGGAAAGGCTCTGGAGATTGTCTCCAAGTTTGTGAAGGAGCGCATGAATGAGAGGAAGATGGAGGGTGCAGAGCATCAGAAAAATGGTGGAAACTTCTTGGATGTTGTGCTTGAACTAATGGAAGGAAGTGGGACTGATGAACCACCCGAGATTTCTGATCATAATCTCAACATTTTCATACTGGAAATATTTATGGCGGGTTCAGAAACAACAAGCAGCACAATAGAATGGGCACTCACCGAGCTTTTATGGAACCCAGAGGTGCTAATGAAGGCCAAATCCGAGCTCAATCAAGTCATAGGTCGAAACAAAAAGATCGAAGAGAGTGACATTGACAATCTTCCCTACTTACAATGCGTAATCAAAGAAACTCTACGATTGCATCCGCCACTTCCATTCCTAGTACCACGAAAGGCAATGCACGACACTGTTTTTAGCGGATACTCGATACCGAAGAACACTCAGGTATTTGTGAATGTTTATGCAATAGGAAGAGACCCAGACGTGTGGAGTGACCCCGTATCGTTTAAGCCTGAGAGGTTCATAGGATCAAAAATTGATTACAAGGGGCAACATTATGAGTTGATTCCATTTGGAGCTGGGAGACGAATGTGTGCGGGTGTGCCATTGGGTCATAGAATGTTGCACCTAACTTTGGGGCTGTTACTTCACCAATTTGATTGGGAATTTGATGGTAGTATTACTAGAGAGACTATGGACTGGAATGAAAGGTTAGGAATGACAATGCGAAAGCATGAGCCATTACTAGCAGTGCCTAAAAAGGCTGAGATCAATTTGCCTTCACTCTCCTTGAAGTTGGGTATGCGACATACAAAAACTGCTTTATACCTGCCATACTTGGTTTCAAACAAGATATTTAAAGCCACGATCGAGCAGTCATCAATGCATATTCCTCGCATAAGGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

503

Amino Acids

57.81

Weight (kDa)

9.1

Isoelectric Point (pI)

45.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 1 - 439 1.9e-106 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 149
Acc36I ACCTGC 1 cut(s) 1440
AccB1I GGYRCC 1 cut(s) 65
AccI GTMKAC 1 cut(s) 92
AclI AACGTT 1 cut(s) 399
AclWI GGATC 4 cut(s) 220, 332, 585, 1138
AcoI YGGCCR 2 cut(s) 102, 513
AcsI RAATTY 1 cut(s) 1268
AcuI CTGAAG 1 cut(s) 554
AcyI GRCGYC 2 cut(s) 235, 284
AfaI GTAC 2 cut(s) 38, 987
AfiI CCNNNNNNNGG 1 cut(s) 1237
AflIII ACRYGT 2 cut(s) 252, 1086
AgsI TTSAA 6 cut(s) 118, 397, 559, 695, 1396, 1449
AhdI GACNNNNNGTC 2 cut(s) 283, 316
AjiI CACGTC 1 cut(s) 1087
AjnI CCWGG 1 cut(s) 411
AluBI AGCT 3 cut(s) 826, 868, 1181
AluI AGCT 3 cut(s) 826, 868, 1181
Alw21I GWGCWC 1 cut(s) 870
Alw26I GTCTC 5 cut(s) 226, 607, 1071, 1180, 1286
AlwI GGATC 4 cut(s) 220, 332, 585, 1138
Ama87I CYCGRG 1 cut(s) 728
AoxI GGCC 3 cut(s) 102, 513, 855
ApeKI GCWGC 1 cut(s) 798
ApoI RAATTY 1 cut(s) 1268
ArsI GACNNNNNNTTYG 2 cut(s) 119, 151
AspLEI GCGC 2 cut(s) 305, 624
AspS9I GGNCC 3 cut(s) 41, 142, 437
AsuC2I CCSGG 1 cut(s) 470
AsuHPI GGTGA 2 cut(s) 811, 1244
AvaI CYCGRG 1 cut(s) 728
AvaII GGWCC 3 cut(s) 41, 142, 437
BaeGI GKGCMC 1 cut(s) 817
BanI GGYRCC 1 cut(s) 65
BanII GRGCYC 1 cut(s) 870
Bbv12I GWGCWC 1 cut(s) 870
BbvI GCAGC 1 cut(s) 810
BccI CCATC 5 cut(s) 73, 451, 481, 634, 1268
BceAI ACGGC 2 cut(s) 150, 297
BcgI CGANNNNNNTGC 2 cut(s) 1478, 1512
BciT130I CCWGG 1 cut(s) 413
BciVI GTATCC 1 cut(s) 1016
BclI TGATCA 1 cut(s) 739
BcnI CCSGG 1 cut(s) 470
BcoDI GTCTC 5 cut(s) 226, 607, 1071, 1180, 1286
BfaI CTAG 3 cut(s) 983, 1287, 1349
BfuAI ACCTGC 1 cut(s) 1440
BfuI GTATCC 1 cut(s) 1016
BisI GCNGC 3 cut(s) 31, 102, 799
BlsI GCNGC 3 cut(s) 32, 103, 800
Bme1390I CCNGG 2 cut(s) 413, 470
Bme18I GGWCC 3 cut(s) 41, 142, 437
BmeRI GACNNNNNGTC 2 cut(s) 283, 316
BmeT110I CYCGRG 1 cut(s) 728
BmgBI CACGTC 1 cut(s) 1087
BmgT120I GGNCC 3 cut(s) 41, 142, 437
BmiI GGNNCC 5 cut(s) 67, 410, 438, 439, 836
BmrFI CCNGG 2 cut(s) 413, 470
BmrI ACTGGG 1 cut(s) 71
BmsI GCATC 4 cut(s) 237, 279, 664, 973
BmuI ACTGGG 1 cut(s) 71
BplI GAGNNNNNCTC 2 cut(s) 587, 619
BpmI CTGGAG 1 cut(s) 614
BpuMI CCSGG 1 cut(s) 470
BsaBI GATNNNNATC 1 cut(s) 744
BsaHI GRCGYC 2 cut(s) 235, 284
BsaI GGTCTC 1 cut(s) 1071
BsaJI CCNNGG 1 cut(s) 527
BsaWI WCCGGW 1 cut(s) 43
BsaXI ACNNNNNCTCC 2 cut(s) 351, 381
Bsc4I CCNNNNNNNGG 1 cut(s) 1237
Bse1I ACTGG 4 cut(s) 66, 224, 768, 1307
Bse3DI GCAATG 1 cut(s) 1005
Bse8I GATNNNNATC 1 cut(s) 744
BseBI CCWGG 1 cut(s) 413
BseDI CCNNGG 1 cut(s) 527
BseGI GGATG 3 cut(s) 462, 688, 964
BseJI GATNNNNATC 1 cut(s) 744
BseLI CCNNNNNNNGG 1 cut(s) 1237
BseMI GCAATG 1 cut(s) 1005
BseMII CTCAG 3 cut(s) 1058, 1107, 1359
BseNI ACTGG 4 cut(s) 66, 224, 768, 1307
BseSI GKGCMC 1 cut(s) 817
BseXI GCAGC 1 cut(s) 810
BseYI CCCAGC 1 cut(s) 1181
BsgI GTGCAG 1 cut(s) 669
Bsh1285I CGRYCG 1 cut(s) 1473
BshFI GGCC 3 cut(s) 104, 515, 857
BshNI GGYRCC 1 cut(s) 65
BsiEI CGRYCG 1 cut(s) 1473
BsiHKAI GWGCWC 1 cut(s) 870
BsiHKCI CYCGRG 1 cut(s) 728
BsiSI CCGG 2 cut(s) 44, 469
BslFI GGGAC 3 cut(s) 440, 450, 727
BslI CCNNNNNNNGG 1 cut(s) 1237
BsmAI GTCTC 5 cut(s) 226, 607, 1071, 1180, 1286
BsmBI CGTCTC 2 cut(s) 226, 1180
BsmFI GGGAC 3 cut(s) 440, 450, 727
BsnI GGCC 3 cut(s) 104, 515, 857
Bso31I GGTCTC 1 cut(s) 1071
BsoBI CYCGRG 1 cut(s) 728
Bsp1286I GDGCHC 2 cut(s) 817, 870
Bsp143I GATC 8 cut(s) 212, 324, 577, 739, 897, 1130, 1371, 1470
BspANI GGCC 3 cut(s) 104, 515, 857
BspCNI CTCAG 3 cut(s) 1057, 1108, 1360
BspHI TCATGA 1 cut(s) 387
BspLI GGNNCC 5 cut(s) 67, 410, 438, 439, 836
BspMI ACCTGC 1 cut(s) 1440
BspPI GGATC 4 cut(s) 220, 332, 585, 1138
BspT107I GGYRCC 1 cut(s) 65
BspTNI GGTCTC 1 cut(s) 1071
BsrDI GCAATG 1 cut(s) 1005
BsrI ACTGG 4 cut(s) 66, 224, 768, 1307
BssECI CCNNGG 1 cut(s) 527
BssMI GATC 8 cut(s) 212, 324, 577, 739, 897, 1130, 1371, 1470
BssNI GRCGYC 2 cut(s) 235, 284
Bst2UI CCWGG 1 cut(s) 413
Bst4CI ACNGT 4 cut(s) 41, 90, 151, 1012
Bst6I CTCTTC 5 cut(s) 324, 554, 566, 897, 1069
BstACI GRCGYC 2 cut(s) 235, 284
BstC8I GCNNGC 2 cut(s) 195, 504
BstDEI CTNAG 5 cut(s) 26, 56, 1044, 1116, 1368
BstDSI CCRYGG 1 cut(s) 527
BstF5I GGATG 3 cut(s) 462, 688, 964
BstHHI GCGC 2 cut(s) 305, 624
BstKTI GATC 8 cut(s) 215, 327, 580, 742, 900, 1133, 1374, 1473
BstMAI GTCTC 5 cut(s) 226, 607, 1071, 1180, 1286
BstMBI GATC 8 cut(s) 212, 324, 577, 739, 897, 1130, 1371, 1470
BstMCI CGRYCG 1 cut(s) 1473
BstMWI GCNNNNNNNGC 3 cut(s) 512, 538, 547
BstNI CCWGG 1 cut(s) 413
BstNSI RCATGY 2 cut(s) 256, 506
BstSCI CCNGG 2 cut(s) 411, 468
BstSLI GKGCMC 1 cut(s) 817
BstV1I GCAGC 1 cut(s) 810
BstX2I RGATCY 2 cut(s) 212, 577
BstXI CCANNNNNNTGG 1 cut(s) 1089
BstYI RGATCY 2 cut(s) 212, 577
BsuI GTATCC 1 cut(s) 1016
BsuRI GGCC 3 cut(s) 104, 515, 857
BtgI CCRYGG 1 cut(s) 527
BtrI CACGTC 1 cut(s) 1087
BtsCI GGATG 3 cut(s) 462, 688, 964
BtsI GCAGTG 1 cut(s) 1359
BtsIMutI CAGTG 3 cut(s) 217, 1008, 1359
BveI ACCTGC 1 cut(s) 1440
Cac8I GCNNGC 2 cut(s) 195, 504
CciI TCATGA 1 cut(s) 387
CfoI GCGC 2 cut(s) 305, 624
Cfr13I GGNCC 3 cut(s) 41, 142, 437
CpoI CGGWCCG 1 cut(s) 41
CseI GACGC 3 cut(s) 243, 273, 327
CsiI ACCWGGT 1 cut(s) 411
Csp6I GTAC 2 cut(s) 37, 986
CspI CGGWCCG 1 cut(s) 41
CviAII CATG 9 cut(s) 109, 253, 356, 388, 479, 503, 511, 625, 1337
CviQI GTAC 2 cut(s) 37, 986
DdeI CTNAG 5 cut(s) 26, 56, 1044, 1116, 1368
DpnI GATC 8 cut(s) 214, 326, 579, 741, 899, 1132, 1373, 1472
DpnII GATC 8 cut(s) 212, 324, 577, 739, 897, 1130, 1371, 1470
DraI TTTAAA 1 cut(s) 1462
DrdI GACNNNNNNGTC 1 cut(s) 149
DriI GACNNNNNGTC 2 cut(s) 283, 316
DseDI GACNNNNNNGTC 1 cut(s) 149
EaeI YGGCCR 2 cut(s) 102, 513
Eam1104I CTCTTC 5 cut(s) 324, 554, 566, 897, 1069
Eam1105I GACNNNNNGTC 2 cut(s) 283, 316
EarI CTCTTC 5 cut(s) 324, 554, 566, 897, 1069
EciI GGCGGA 1 cut(s) 957
Ecl136II GAGCTC 1 cut(s) 868
Eco24I GRGCYC 1 cut(s) 870
Eco31I GGTCTC 1 cut(s) 1071
Eco47I GGWCC 3 cut(s) 41, 142, 437
Eco53kI GAGCTC 1 cut(s) 868
Eco57I CTGAAG 1 cut(s) 554
Eco88I CYCGRG 1 cut(s) 728
EcoICRI GAGCTC 1 cut(s) 868
EcoO109I RGGNCCY 1 cut(s) 437
EcoRII CCWGG 1 cut(s) 411
EcoT22I ATGCAT 1 cut(s) 1491
EcoT38I GRGCYC 1 cut(s) 870
Esp3I CGTCTC 2 cut(s) 226, 1180
FaeI CATG 9 cut(s) 112, 256, 359, 391, 482, 506, 514, 628, 1340
FaqI GGGAC 3 cut(s) 440, 450, 727
FatI CATG 9 cut(s) 108, 252, 355, 387, 478, 502, 510, 624, 1336
FauI CCCGC 4 cut(s) 216, 555, 772, 1192
FbaI TGATCA 1 cut(s) 739
FblI GTMKAC 1 cut(s) 92
Fnu4HI GCNGC 3 cut(s) 31, 102, 799
FokI GGATG 3 cut(s) 469, 695, 951
FriOI GRGCYC 1 cut(s) 870
Fsp4HI GCNGC 3 cut(s) 31, 102, 799
FspBI CTAG 3 cut(s) 983, 1287, 1349
GlaI GCGC 2 cut(s) 304, 623
GluI GCNGC 3 cut(s) 31, 102, 799
GsaI CCCAGC 1 cut(s) 1185
GsuI CTGGAG 1 cut(s) 614
HaeIII GGCC 3 cut(s) 104, 515, 857
HapII CCGG 2 cut(s) 44, 469
HgaI GACGC 3 cut(s) 243, 273, 327
HhaI GCGC 2 cut(s) 305, 624
Hin1I GRCGYC 2 cut(s) 235, 284
Hin1II CATG 9 cut(s) 112, 256, 359, 391, 482, 506, 514, 628, 1340
Hin6I GCGC 2 cut(s) 303, 622
HinP1I GCGC 2 cut(s) 303, 622
HincII GTYRAC 1 cut(s) 262
HindII GTYRAC 1 cut(s) 262
HinfI GANTC 1 cut(s) 1168
HpaII CCGG 2 cut(s) 44, 469
HphI GGTGA 2 cut(s) 811, 1244
Hpy166II GTNNAC 3 cut(s) 93, 142, 262
Hpy188I TCNGA 6 cut(s) 297, 448, 660, 739, 787, 865
Hpy188III TCNNGA 4 cut(s) 244, 388, 406, 593
Hpy8I GTNNAC 3 cut(s) 93, 142, 262
Hpy99I CGWCG 5 cut(s) 297, 311, 343, 346, 349
HpyAV CCTTC 5 cut(s) 446, 610, 698, 847, 1392
HpyCH4III ACNGT 4 cut(s) 41, 90, 151, 1012
HpyCH4IV ACGT 4 cut(s) 373, 392, 399, 1086
HpyF10VI GCNNNNNNNGC 3 cut(s) 512, 538, 547
HpyF3I CTNAG 5 cut(s) 26, 56, 1044, 1116, 1368
HpySE526I ACGT 4 cut(s) 373, 392, 399, 1086
Hsp92I GRCGYC 2 cut(s) 235, 284
Hsp92II CATG 9 cut(s) 112, 256, 359, 391, 482, 506, 514, 628, 1340
HspAI GCGC 2 cut(s) 303, 622
KflI GGGWCCC 1 cut(s) 437
Ksp22I TGATCA 1 cut(s) 739
Kzo9I GATC 8 cut(s) 212, 324, 577, 739, 897, 1130, 1371, 1470
LmnI GCTCC 2 cut(s) 619, 1178
Lsp1109I GCAGC 1 cut(s) 810
LweI GCATC 4 cut(s) 237, 279, 664, 973
MabI ACCWGGT 1 cut(s) 411
MaeI CTAG 3 cut(s) 983, 1287, 1349
MaeII ACGT 4 cut(s) 373, 392, 399, 1086
MaeIII GTNAC 3 cut(s) 908, 1094, 1245
MalI GATC 8 cut(s) 214, 326, 579, 741, 899, 1132, 1373, 1472
MboI GATC 8 cut(s) 212, 324, 577, 739, 897, 1130, 1371, 1470
MboII GAAGA 9 cut(s) 341, 571, 577, 583, 649, 914, 914, 1048, 1086
MflI RGATCY 2 cut(s) 212, 577
MhlI GDGCHC 2 cut(s) 817, 870
MluCI AATT 4 cut(s) 1137, 1256, 1268, 1375
MmeI TCCRAC 1 cut(s) 275
Mph1103I ATGCAT 1 cut(s) 1491
MseI TTAA 2 cut(s) 1110, 1461
MspI CCGG 2 cut(s) 44, 469
MspR9I CCNGG 2 cut(s) 413, 470
MvaI CCWGG 1 cut(s) 413
MwoI GCNNNNNNNGC 3 cut(s) 512, 538, 547
NciI CCSGG 1 cut(s) 470
NdeII GATC 8 cut(s) 212, 324, 577, 739, 897, 1130, 1371, 1470
NlaIII CATG 9 cut(s) 112, 256, 359, 391, 482, 506, 514, 628, 1340
NlaIV GGNNCC 5 cut(s) 67, 410, 438, 439, 836
NmuCI GTSAC 2 cut(s) 908, 1094
NsiI ATGCAT 1 cut(s) 1491
NspI RCATGY 2 cut(s) 256, 506
PaeI GCATGC 1 cut(s) 506
PagI TCATGA 1 cut(s) 387
PciI ACATGT 1 cut(s) 252
PfeI GAWTC 1 cut(s) 1168
PkrI GCNGC 3 cut(s) 32, 103, 800
Ple19I CGATCG 1 cut(s) 1473
PpuMI RGGWCCY 1 cut(s) 437
PscI ACATGT 1 cut(s) 252
Psp124BI GAGCTC 1 cut(s) 870
Psp1406I AACGTT 1 cut(s) 399
Psp5II RGGWCCY 1 cut(s) 437
Psp6I CCWGG 1 cut(s) 411
PspFI CCCAGC 1 cut(s) 1181
PspGI CCWGG 1 cut(s) 411
PspN4I GGNNCC 5 cut(s) 67, 410, 438, 439, 836
PspPI GGNCC 3 cut(s) 41, 142, 437
PspPPI RGGWCCY 1 cut(s) 437
PsuI RGATCY 2 cut(s) 212, 577
PvuI CGATCG 1 cut(s) 1473
RsaI GTAC 2 cut(s) 38, 987
RsaNI GTAC 2 cut(s) 37, 986
Rsr2I CGGWCCG 1 cut(s) 41
RsrII CGGWCCG 1 cut(s) 41
SacI GAGCTC 1 cut(s) 870
SaqAI TTAA 2 cut(s) 1110, 1461
SatI GCNGC 3 cut(s) 31, 102, 799
Sau3AI GATC 8 cut(s) 212, 324, 577, 739, 897, 1130, 1371, 1470
Sau96I GGNCC 3 cut(s) 41, 142, 437
ScrFI CCNGG 2 cut(s) 413, 470
SduI GDGCHC 2 cut(s) 817, 870
SexAI ACCWGGT 1 cut(s) 411
SfaNI GCATC 4 cut(s) 237, 279, 664, 973
SinI GGWCC 3 cut(s) 41, 142, 437
SphI GCATGC 1 cut(s) 506
Sse9I AATT 4 cut(s) 1137, 1256, 1268, 1375
SspI AATATT 1 cut(s) 771
SspMI CTAG 3 cut(s) 983, 1287, 1349
SstI GAGCTC 1 cut(s) 870
StyD4I CCNGG 2 cut(s) 411, 468
TaaI ACNGT 4 cut(s) 41, 90, 151, 1012
TaiI ACGT 4 cut(s) 376, 395, 402, 1089
TaqI TCGA 6 cut(s) 327, 422, 886, 900, 1028, 1473
TasI AATT 4 cut(s) 1137, 1256, 1268, 1375
TauI GCSGC 2 cut(s) 33, 104
TfiI GAWTC 1 cut(s) 1168
Tru1I TTAA 2 cut(s) 1110, 1461
Tru9I TTAA 2 cut(s) 1110, 1461
TscAI CASTG 3 cut(s) 224, 1015, 1359
TseFI GTSAC 2 cut(s) 908, 1094
TseI GCWGC 1 cut(s) 798
Tsp45I GTSAC 2 cut(s) 908, 1094
TspDTI ATGAA 8 cut(s) 171, 584, 641, 735, 748, 866, 1114, 1323
TspGWI ACGGA 2 cut(s) 225, 516
TspRI CASTG 3 cut(s) 224, 1015, 1359
VpaK11BI GGWCC 3 cut(s) 41, 142, 437
XapI RAATTY 1 cut(s) 1268
XceI RCATGY 2 cut(s) 256, 506
XcmI CCANNNNNNNNNTGG 1 cut(s) 76
XmiI GTMKAC 1 cut(s) 92
XspI CTAG 3 cut(s) 983, 1287, 1349
Zsp2I ATGCAT 1 cut(s) 1491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.