RLG00000024129

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0006414
Physical Location & Seq
Reverse (-)
32241179 .. 32242828
1650 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024129

Sequence Viewer

Length: 1548 bp
ATGGAGCACTTGCCCTGGAATTTTCTTGTTTATTTTATCATACCAGCTCTGCTCTTCCTGTTCATCCTCCGGAGATCAAACTCCAGTAAAGATCATGAGCTTCCACCCGGCCCACCGGGATGGCCAATAATCGGCAACATGCTCTACCTTGGAGAGATGCCACACAAAACCCTCAACAAGCTGCGTCACACGTTTGGTCCGGTGATATGGCTAAGAATTGGTGCCCGAAACACCATGGCAATTCTCTCCGCCAAATCAGCCACCGAGTTTTTCAAAAACCATGACCTCTCGTTCGCTGAACGCTCCATCACCGAAGGTAGTCGAGTGCTCGACTACCATAAGGGGTCACTAGCTCTGGCCCCTTATGGGTCACACTGGCGCGTGTTAAAGCGCCTGTTGACGGTTGACATGTTGACCAACAAGCGTATCAATGACACTGCACCGATTCGGAGAAAGTGCGTTGACATCATGCTGACATGGATAGAAGAGGAAGCCTCTAAACTCCAAGCGAATGGAACATCATCGCGTGGGGTGCACCTGGCTCGTTTCGTGTTCCTCATGGCGTTTAATCTTCTTGGGAATCTCCTGTTGTCTAGAGACTTGGTGGATCCGAATTCGAAAGAAGGGCTGGAGTTTTTCGAAGTGATGAAGGGACTCATGGAGACGAATGGGCATGCCAACATGGCGGACTACTTTCCATGGCTGAGGTGGCTTGATCCACAAGGTGTGAAGAAGAGGATGAAAAAGGATATGGCAAAGGCTCTGAAACTAGCCTCTGGTTTTGTGAAGATGCGCTTGAATGATAGGCAGTTGCTGGGTGGAGAAAAAACTAGGGACTTTCTTGATGTGTTGCTTGAATTTGAAGGCAACGGAAATGATGAACCAGCAAAGATTTCTGACCATGATATCAACATTTTCATACTGGAAATTTTTTTGGCAGGTGCAGAAACAACCAGCAGCACAATTGAATGGGCAATGACCGAGCTACTATGCAACCCGGAGACGCTAATCAAGGCCAAAAGAGAACTCAATCATGTGATCGGTCTAAACAGAAAGATCGACGAGTCTGACGTTGACAATCTTCCATACTTGCAAGGCATAGTTAAAGAAACACTAAGATTACATCCACCCGTCCCATTCCTCATACCAAGGAAAGCTATGCAAGACACCAAATTCATGGGTTACTTCATACCCAAAAATACACAGGTATTTGTTAATGCTTATGCAATTGGAAGAGACCCAGATGTTTGGATTGATGAGCCTAATTCGTTTAAGCCCGAGAGGTTCATAGGCTCAAAAGTGGATTATAAGGGTCAGCATTATGAGTTGATTCCATTTGGAGCCGGGAGAAGAATGTGTGCTGGTGTGCCGTTGGGTGATAAGATGCTGCACCTTACTTTGGGGTCATTGCTTCACCAATTTGATTGGGCACTTGATGGAAATGTTACTAAGGAAACTATGGATTGGAATGATAGGTTAGGCATAACAATGAGAAAGTATCAGCCATTAGTAGCTGTGCCTACAAAGTGCTTGGCTAGTTCATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

516

Amino Acids

58.71

Weight (kDa)

8.88

Isoelectric Point (pI)

36.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 35 - 499 3.5e-104 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1308
AarI CACCTGC 1 cut(s) 929
Acc36I ACCTGC 1 cut(s) 929
AccB1I GGYRCC 1 cut(s) 221
AccII CGCG 2 cut(s) 381, 526
AccIII TCCGGA 1 cut(s) 69
AciI CCGC 2 cut(s) 249, 686
AclWI GGATC 3 cut(s) 602, 615, 710
AcoI YGGCCR 1 cut(s) 122
AcsI RAATTY 5 cut(s) 19, 613, 857, 927, 1172
AdeI CACNNNGTG 1 cut(s) 725
AfiI CCNNNNNNNGG 4 cut(s) 131, 366, 400, 1399
AflIII ACRYGT 2 cut(s) 189, 408
AgsI TTSAA 5 cut(s) 274, 799, 857, 863, 968
AjnI CCWGG 2 cut(s) 14, 537
AluBI AGCT 7 cut(s) 47, 100, 181, 353, 985, 1157, 1514
AluI AGCT 7 cut(s) 47, 100, 181, 353, 985, 1157, 1514
Alw21I GWGCWC 3 cut(s) 9, 330, 537
Alw26I GTCTC 4 cut(s) 591, 656, 995, 1230
Alw44I GTGCAC 1 cut(s) 533
AlwI GGATC 3 cut(s) 602, 615, 710
AlwNI CAGNNNCTG 1 cut(s) 814
Ama87I CYCGRG 1 cut(s) 1277
Aor13HI TCCGGA 1 cut(s) 69
AoxI GGCC 4 cut(s) 109, 122, 357, 1014
ApaLI GTGCAC 1 cut(s) 533
ApeKI GCWGC 3 cut(s) 181, 957, 1387
ApoI RAATTY 5 cut(s) 19, 613, 857, 927, 1172
ArsI GACNNNNNNTTYG 2 cut(s) 275, 307
AspLEI GCGC 3 cut(s) 381, 393, 795
AspS9I GGNCC 3 cut(s) 110, 197, 358
AsuC2I CCSGG 4 cut(s) 108, 117, 998, 1345
AsuHPI GGTGA 4 cut(s) 214, 301, 1388, 1406
AsuII TTCGAA 2 cut(s) 617, 639
AvaI CYCGRG 1 cut(s) 1277
AvaII GGWCC 1 cut(s) 197
BaeGI GKGCMC 3 cut(s) 226, 537, 1432
BaeI ACNNNNGTAYC 2 cut(s) 409, 442
BalI TGGCCA 1 cut(s) 124
BamHI GGATCC 1 cut(s) 607
BanI GGYRCC 1 cut(s) 221
Bbv12I GWGCWC 3 cut(s) 9, 330, 537
BbvCI CCTCAGC 1 cut(s) 704
BbvI GCAGC 3 cut(s) 168, 969, 1374
BccI CCATC 3 cut(s) 114, 314, 1430
BceAI ACGGC 1 cut(s) 1354
BcgI CGANNNNNNTGC 2 cut(s) 524, 558
BciT130I CCWGG 2 cut(s) 16, 539
BcnI CCSGG 4 cut(s) 108, 117, 998, 1345
BcoDI GTCTC 4 cut(s) 591, 656, 995, 1230
BfaI CTAG 5 cut(s) 350, 594, 770, 831, 1536
BfoI RGCGCY 1 cut(s) 394
BfuAI ACCTGC 1 cut(s) 929
BglI GCCNNNNNGGC 1 cut(s) 683
BisI GCNGC 3 cut(s) 182, 958, 1388
BlsI GCNGC 3 cut(s) 183, 959, 1389
Bme1390I CCNGG 6 cut(s) 16, 108, 117, 539, 998, 1345
Bme18I GGWCC 1 cut(s) 197
BmeT110I CYCGRG 1 cut(s) 1277
BmgT120I GGNCC 3 cut(s) 110, 197, 358
BmiI GGNNCC 4 cut(s) 223, 360, 609, 1342
BmrFI CCNGG 6 cut(s) 16, 108, 117, 539, 998, 1345
BmsI GCATC 3 cut(s) 147, 780, 1374
BplI GAGNNNNNCTC 2 cut(s) 479, 511
BpmI CTGGAG 2 cut(s) 67, 650
Bpu10I CCTNAGC 1 cut(s) 704
Bpu14I TTCGAA 2 cut(s) 617, 639
BpuMI CCSGG 4 cut(s) 108, 117, 998, 1345
BsaI GGTCTC 1 cut(s) 1230
BsaJI CCNNGG 5 cut(s) 14, 148, 234, 698, 1148
BsaWI WCCGGW 2 cut(s) 69, 199
Bsc4I CCNNNNNNNGG 4 cut(s) 131, 366, 400, 1399
Bse1I ACTGG 3 cut(s) 84, 380, 927
Bse3DI GCAATG 2 cut(s) 981, 1406
BseAI TCCGGA 1 cut(s) 69
BseBI CCWGG 2 cut(s) 16, 539
BseDI CCNNGG 5 cut(s) 14, 148, 234, 698, 1148
BseGI GGATG 4 cut(s) 63, 125, 744, 1123
BseLI CCNNNNNNNGG 4 cut(s) 131, 366, 400, 1399
BseMI GCAATG 2 cut(s) 981, 1406
BseMII CTCAG 1 cut(s) 695
BseNI ACTGG 3 cut(s) 84, 380, 927
BseSI GKGCMC 3 cut(s) 226, 537, 1432
BseXI GCAGC 3 cut(s) 168, 969, 1374
BseYI CCCAGC 1 cut(s) 814
BsgI GTGCAG 3 cut(s) 423, 963, 1373
Bsh1236I CGCG 2 cut(s) 381, 526
BshFI GGCC 4 cut(s) 111, 124, 359, 1016
BshNI GGYRCC 1 cut(s) 221
BsiHKAI GWGCWC 3 cut(s) 9, 330, 537
BsiHKCI CYCGRG 1 cut(s) 1277
BsiSI CCGG 6 cut(s) 70, 108, 116, 200, 998, 1344
BslFI GGGAC 3 cut(s) 666, 848, 1118
BslI CCNNNNNNNGG 4 cut(s) 131, 366, 400, 1399
BsmAI GTCTC 4 cut(s) 591, 656, 995, 1230
BsmBI CGTCTC 2 cut(s) 656, 995
BsmFI GGGAC 3 cut(s) 666, 848, 1118
BsnI GGCC 4 cut(s) 111, 124, 359, 1016
Bso31I GGTCTC 1 cut(s) 1230
BsoBI CYCGRG 1 cut(s) 1277
Bsp119I TTCGAA 2 cut(s) 617, 639
Bsp1286I GDGCHC 5 cut(s) 9, 226, 330, 537, 1432
Bsp13I TCCGGA 1 cut(s) 69
Bsp143I GATC 6 cut(s) 74, 91, 607, 715, 1038, 1056
Bsp19I CCATGG 2 cut(s) 234, 698
BspACI CCGC 2 cut(s) 249, 686
BspANI GGCC 4 cut(s) 111, 124, 359, 1016
BspCNI CTCAG 1 cut(s) 696
BspEI TCCGGA 1 cut(s) 69
BspFNI CGCG 2 cut(s) 381, 526
BspHI TCATGA 1 cut(s) 94
BspLI GGNNCC 4 cut(s) 223, 360, 609, 1342
BspMI ACCTGC 1 cut(s) 929
BspPI GGATC 3 cut(s) 602, 615, 710
BspQI GCTCTTC 1 cut(s) 59
BspT104I TTCGAA 2 cut(s) 617, 639
BspT107I GGYRCC 1 cut(s) 221
BspTNI GGTCTC 1 cut(s) 1230
BsrDI GCAATG 2 cut(s) 981, 1406
BsrI ACTGG 3 cut(s) 84, 380, 927
BssECI CCNNGG 5 cut(s) 14, 148, 234, 698, 1148
BssMI GATC 6 cut(s) 74, 91, 607, 715, 1038, 1056
BssT1I CCWWGG 4 cut(s) 148, 234, 698, 1148
Bst2UI CCWGG 2 cut(s) 16, 539
Bst4CI ACNGT 1 cut(s) 403
Bst6I CTCTTC 4 cut(s) 59, 480, 728, 1228
BstBI TTCGAA 2 cut(s) 617, 639
BstC8I GCNNGC 1 cut(s) 675
BstDEI CTNAG 4 cut(s) 212, 704, 1115, 1449
BstDSI CCRYGG 2 cut(s) 234, 698
BstF5I GGATG 4 cut(s) 63, 125, 744, 1123
BstFNI CGCG 2 cut(s) 381, 526
BstH2I RGCGCY 1 cut(s) 394
BstHHI GCGC 3 cut(s) 381, 393, 795
BstKTI GATC 6 cut(s) 77, 94, 610, 718, 1041, 1059
BstMAI GTCTC 4 cut(s) 591, 656, 995, 1230
BstMBI GATC 6 cut(s) 74, 91, 607, 715, 1038, 1056
BstMWI GCNNNNNNNGC 4 cut(s) 257, 532, 683, 709
BstNI CCWGG 2 cut(s) 16, 539
BstNSI RCATGY 3 cut(s) 142, 412, 677
BstSCI CCNGG 6 cut(s) 14, 106, 115, 537, 996, 1343
BstSLI GKGCMC 3 cut(s) 226, 537, 1432
BstUI CGCG 2 cut(s) 381, 526
BstV1I GCAGC 3 cut(s) 168, 969, 1374
BstX2I RGATCY 1 cut(s) 607
BstXI CCANNNNNNTGG 4 cut(s) 120, 512, 1177, 1248
BstYI RGATCY 1 cut(s) 607
BsuRI GGCC 4 cut(s) 111, 124, 359, 1016
BtgI CCRYGG 2 cut(s) 234, 698
BtgZI GCGATG 1 cut(s) 507
BtsCI GGATG 4 cut(s) 63, 125, 744, 1123
BtsI GCAGTG 1 cut(s) 435
BtsIMutI CAGTG 2 cut(s) 373, 435
BveI ACCTGC 1 cut(s) 929
Cac8I GCNNGC 1 cut(s) 675
CaiI CAGNNNCTG 1 cut(s) 814
CciI TCATGA 1 cut(s) 94
CfoI GCGC 3 cut(s) 381, 393, 795
Cfr13I GGNCC 3 cut(s) 110, 197, 358
CseI GACGC 2 cut(s) 173, 1012
DdeI CTNAG 4 cut(s) 212, 704, 1115, 1449
DpnI GATC 6 cut(s) 76, 93, 609, 717, 1040, 1058
DpnII GATC 6 cut(s) 74, 91, 607, 715, 1038, 1056
DraIII CACNNNGTG 1 cut(s) 725
EaeI YGGCCR 1 cut(s) 122
Eam1104I CTCTTC 4 cut(s) 59, 480, 728, 1228
EarI CTCTTC 4 cut(s) 59, 480, 728, 1228
EciI GGCGGA 2 cut(s) 238, 701
Eco130I CCWWGG 4 cut(s) 148, 234, 698, 1148
Eco31I GGTCTC 1 cut(s) 1230
Eco32I GATATC 1 cut(s) 907
Eco47I GGWCC 1 cut(s) 197
Eco88I CYCGRG 1 cut(s) 1277
EcoRI GAATTC 1 cut(s) 613
EcoRII CCWGG 2 cut(s) 14, 537
EcoRV GATATC 1 cut(s) 907
EcoT14I CCWWGG 4 cut(s) 148, 234, 698, 1148
ErhI CCWWGG 4 cut(s) 148, 234, 698, 1148
Esp3I CGTCTC 2 cut(s) 656, 995
FalI AAGNNNNNCTT 2 cut(s) 779, 811
FaqI GGGAC 3 cut(s) 666, 848, 1118
Fnu4HI GCNGC 3 cut(s) 182, 958, 1388
FokI GGATG 4 cut(s) 50, 132, 751, 1110
Fsp4HI GCNGC 3 cut(s) 182, 958, 1388
FspBI CTAG 5 cut(s) 350, 594, 770, 831, 1536
GlaI GCGC 3 cut(s) 380, 392, 794
GluI GCNGC 3 cut(s) 182, 958, 1388
GsaI CCCAGC 1 cut(s) 818
GsuI CTGGAG 2 cut(s) 67, 650
HaeII RGCGCY 1 cut(s) 394
HaeIII GGCC 4 cut(s) 111, 124, 359, 1016
HapII CCGG 6 cut(s) 70, 108, 116, 200, 998, 1344
HgaI GACGC 2 cut(s) 173, 1012
HhaI GCGC 3 cut(s) 381, 393, 795
Hin6I GCGC 3 cut(s) 379, 391, 793
HinP1I GCGC 3 cut(s) 379, 391, 793
HincII GTYRAC 5 cut(s) 399, 406, 414, 463, 1075
HindII GTYRAC 5 cut(s) 399, 406, 414, 463, 1075
HinfI GANTC 5 cut(s) 445, 580, 654, 1064, 1330
HpaII CCGG 6 cut(s) 70, 108, 116, 200, 998, 1344
HphI GGTGA 4 cut(s) 214, 301, 1388, 1406
Hpy166II GTNNAC 6 cut(s) 399, 406, 414, 463, 535, 1075
Hpy188I TCNGA 5 cut(s) 450, 612, 765, 898, 1069
Hpy188III TCNNGA 4 cut(s) 70, 95, 594, 842
Hpy8I GTNNAC 6 cut(s) 399, 406, 414, 463, 535, 1075
Hpy99I CGWCG 1 cut(s) 1064
HpyAV CCTTC 4 cut(s) 308, 617, 643, 857
HpyCH4III ACNGT 1 cut(s) 403
HpyCH4IV ACGT 2 cut(s) 191, 1071
HpyCH4V TGCA 8 cut(s) 440, 535, 944, 993, 1093, 1162, 1226, 1390
HpyF10VI GCNNNNNNNGC 4 cut(s) 257, 532, 683, 709
HpyF3I CTNAG 4 cut(s) 212, 704, 1115, 1449
HpySE526I ACGT 2 cut(s) 191, 1071
HspAI GCGC 3 cut(s) 379, 391, 793
Kpn2I TCCGGA 1 cut(s) 69
Kzo9I GATC 6 cut(s) 74, 91, 607, 715, 1038, 1056
LguI GCTCTTC 1 cut(s) 59
LmnI GCTCC 3 cut(s) 4, 308, 1340
Lsp1109I GCAGC 3 cut(s) 168, 969, 1374
LweI GCATC 3 cut(s) 147, 780, 1374
MaeI CTAG 5 cut(s) 350, 594, 770, 831, 1536
MaeII ACGT 2 cut(s) 191, 1071
MaeIII GTNAC 5 cut(s) 185, 345, 369, 1181, 1444
MalI GATC 6 cut(s) 76, 93, 609, 717, 1040, 1058
MboI GATC 6 cut(s) 74, 91, 607, 715, 1038, 1056
MboII GAAGA 9 cut(s) 46, 497, 563, 742, 745, 799, 1073, 1245, 1362
MfeI CAATTG 2 cut(s) 963, 1227
MflI RGATCY 1 cut(s) 607
MhlI GDGCHC 5 cut(s) 9, 226, 330, 537, 1432
MlsI TGGCCA 1 cut(s) 124
MluNI TGGCCA 1 cut(s) 124
MlyI GAGTC 2 cut(s) 648, 1073
Mox20I TGGCCA 1 cut(s) 124
MroI TCCGGA 1 cut(s) 69
MscI TGGCCA 1 cut(s) 124
MseI TTAA 5 cut(s) 386, 567, 1104, 1215, 1272
MslI CAYNNNNRTG 2 cut(s) 118, 1487
Msp20I TGGCCA 1 cut(s) 124
MspI CCGG 6 cut(s) 70, 108, 116, 200, 998, 1344
MspR9I CCNGG 6 cut(s) 16, 108, 117, 539, 998, 1345
MunI CAATTG 2 cut(s) 963, 1227
MvaI CCWGG 2 cut(s) 16, 539
MvnI CGCG 2 cut(s) 381, 526
MwoI GCNNNNNNNGC 4 cut(s) 257, 532, 683, 709
NciI CCSGG 4 cut(s) 108, 117, 998, 1345
NcoI CCATGG 2 cut(s) 234, 698
NdeII GATC 6 cut(s) 74, 91, 607, 715, 1038, 1056
NlaIV GGNNCC 4 cut(s) 223, 360, 609, 1342
NmuCI GTSAC 3 cut(s) 185, 345, 369
NspI RCATGY 3 cut(s) 142, 412, 677
NspV TTCGAA 2 cut(s) 617, 639
PaeI GCATGC 1 cut(s) 677
PagI TCATGA 1 cut(s) 94
PaqCI CACCTGC 1 cut(s) 929
PciI ACATGT 1 cut(s) 408
PciSI GCTCTTC 1 cut(s) 59
PcsI WCGNNNNNNNCGW 1 cut(s) 1068
PfeI GAWTC 3 cut(s) 445, 580, 1330
PkrI GCNGC 3 cut(s) 183, 959, 1389
PleI GAGTC 2 cut(s) 648, 1072
PpsI GAGTC 2 cut(s) 648, 1072
PscI ACATGT 1 cut(s) 408
PsiI TTATAA 1 cut(s) 1308
Psp6I CCWGG 2 cut(s) 14, 537
PspFI CCCAGC 1 cut(s) 814
PspGI CCWGG 2 cut(s) 14, 537
PspN4I GGNNCC 4 cut(s) 223, 360, 609, 1342
PspPI GGNCC 3 cut(s) 110, 197, 358
PstNI CAGNNNCTG 1 cut(s) 814
PsuI RGATCY 1 cut(s) 607
RseI CAYNNNNRTG 2 cut(s) 118, 1487
SapI GCTCTTC 1 cut(s) 59
SaqAI TTAA 5 cut(s) 386, 567, 1104, 1215, 1272
SatI GCNGC 3 cut(s) 182, 958, 1388
Sau3AI GATC 6 cut(s) 74, 91, 607, 715, 1038, 1056
Sau96I GGNCC 3 cut(s) 110, 197, 358
SchI GAGTC 2 cut(s) 648, 1073
ScrFI CCNGG 6 cut(s) 16, 108, 117, 539, 998, 1345
SduI GDGCHC 5 cut(s) 9, 226, 330, 537, 1432
SfaNI GCATC 3 cut(s) 147, 780, 1374
SfuI TTCGAA 2 cut(s) 617, 639
SinI GGWCC 1 cut(s) 197
SmiMI CAYNNNNRTG 2 cut(s) 118, 1487
SphI GCATGC 1 cut(s) 677
SsiI CCGC 2 cut(s) 249, 686
SspMI CTAG 5 cut(s) 350, 594, 770, 831, 1536
StyD4I CCNGG 6 cut(s) 14, 106, 115, 537, 996, 1343
StyI CCWWGG 4 cut(s) 148, 234, 698, 1148
TaaI ACNGT 1 cut(s) 403
TaiI ACGT 2 cut(s) 194, 1074
TaqI TCGA 5 cut(s) 322, 330, 617, 639, 1059
TaqII GACCGA 2 cut(s) 995, 1031
TfiI GAWTC 3 cut(s) 445, 580, 1330
Tru1I TTAA 5 cut(s) 386, 567, 1104, 1215, 1272
Tru9I TTAA 5 cut(s) 386, 567, 1104, 1215, 1272
TscAI CASTG 2 cut(s) 380, 442
TseFI GTSAC 3 cut(s) 185, 345, 369
TseI GCWGC 3 cut(s) 181, 957, 1387
Tsp45I GTSAC 3 cut(s) 185, 345, 369
TspDTI ATGAA 9 cut(s) 52, 662, 755, 894, 907, 1165, 1177, 1276, 1530
TspGWI ACGGA 1 cut(s) 885
TspRI CASTG 2 cut(s) 380, 442
VneI GTGCAC 1 cut(s) 533
VpaK11BI GGWCC 1 cut(s) 197
XapI RAATTY 5 cut(s) 19, 613, 857, 927, 1172
XbaI TCTAGA 1 cut(s) 593
XceI RCATGY 3 cut(s) 142, 412, 677
XcmI CCANNNNNNNNNTGG 1 cut(s) 705
XspI CTAG 5 cut(s) 350, 594, 770, 831, 1536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.