Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0006253

Physical Location & Seq

Genomic Coordinates Forward (+)

17393802 .. 17400634

Gene Structure 6833 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1109700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1509 bp

ATGATCGAAGGCTTAAGACCAAGTACAACAACGCTATCCATGTTATTCAGAGAGCTCTTGCGCTCTACTCGTAATATTGAAGAGGTTGCCTTCAGCTTTAATGGGGGAAAGGATTCAACTGTTTTGTTGCACCTACTCAGGGCTGGCTACTTTTTGCATAAAGGGGAGCAAAGCTGTTCTAACGGGGGTGTAAAATATTTTCCAATGCGAACGATATATTTCGAGTGCCCATCTGCTTTCCCTGAAATCAACTCGTTTACTTATGATACAGCCACTACCTATGGTTTGCAACTGGATATCATTCGCTCTGATTTCAAGTCTGGTTTGGAGGATTTACTAAAATCCAAACCAATCAAAGCTATTTTCCTTGGTGTCCGAATCGGTGATCCTACTGCGGTTGGTCAAGAACAATTTTCTCCCAGTTCAGTTGGATGGCCACCTTTCATGAGGGTGAATCCTATATTGGATTGGTCATACAGAGATGTGTGGGCCTTACTCTTAACTTGCAAAGTGCAGTACTGCAGTCTTTATGATCAAGGTTATACTTCAATTGGAAGCATATATGACACAGTTCCAAATGCATTATTGAGCATTAACAATTCATCTGACAACAAAGAAGTATTTAGACCTGCGTATTTACTTTCTGATGGAAGATTAGAGAGAGCCGGAAGGGTTAAAAAACTTTCTTCTTCGGTTCGTGGGCATCAGTCTGCTGTTATGAATGGCCTTGATGGTGTGGGCTTACATAACAGTAGCCTACTCATGTCCTCAGCCATTGCTGTGGGGGATGAGATTCTGTTTGGCACTGTTGAGGATCAAATAGGGCCTTCATTGTGTAGAAAGCTCCATTCTATTGGCTGGTTGGTATCACAAACTGCTGTTGTTCGGAATGATATAGATGCTGTCGCCGAAGAAGTCGAGCGACGACAATCAACTGATGATATGGTATTTATGTGTGGAGGGGTTGGTCCACTGCACTCAGATGTCACTTTGGCAGGTGTTGCAAAGGCTTTTGCTGTTCGTCTGGCTCCTGATGAAGAATTTGAAGAATATCTAAGGCATCTTATAGGTGATCAATGCACTGGTGACCGGAATGAGATGGCTCTGTTGCCTGAAGGTATCACTGAACTATTGCATCATGAAAAGCTGATTGTCCCTTTGATCAAGTGTAAAAATGTAATAATCCTTACTGCAACAAATATCTTGGAGCTGGATGAGCAGTGGAACTGTCTAATTGAACTAATGAGATCCGATGGTGCACTAGCAACGATGCAACCATTTGTATCTAAGCACTTGACAACAAACCTTACAGATGTAGAAATTGCTCAACCTCTGTCAAAACTTTGCCTTGAATTTCCGGACCTTTACATTGGTTGTCGCCGCAAATCAAGGACGGAGCCTCTTATAATCTACTTCAAGGGCAAGGACCAAGACCGAATTCAATCAGCCATAGAGGCATTGTGCAAGAAGTTCCATCCGGGGGCTTTGGTTGAAATCAACTCAAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003919 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006139 GO:0006725 GO:0006732 GO:0006747 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009117 GO:0009165 GO:0009987 GO:0016020 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0019637 GO:0034641 GO:0034654 GO:0042726 GO:0042727 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046443 GO:0046483 GO:0051186 GO:0051188 GO:0055086 GO:0055114 GO:0070566 GO:0071704 GO:0071944 GO:0072387 GO:0072388 GO:0090407 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00740 ko01100 ko01110 map00740 map01100 map01110

Pfam Domains

Protein Families

MoCF_biosynth PAPS_reduct

Protein Analysis

503

Amino Acids

55.79

Weight (kDa)

5.57

Isoelectric Point (pI)

47.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PAPS_reduct	PF01507	29 - 191	1.5e-20	Phosphoadenosine phosphosulfate reductase domain
MoCF_biosynth	PF00994	259 - 358	1.1e-17	Probable molybdopterin binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006253)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G03430
fragaria_vesca	FvH4_6g16530 FvH4_6g16530 FvH4_6g16530 FvH4_6g16530 FvH4_6g16530 FvH4_6g16530 FvH4_6g16530 FvH4_6g16530 FvH4_6g16530 FvH4_6g16530
malus_domestica	MD04G1091700.v1.1 MD12G1109700.v1.1
prunus_persica	Prupe.7G128300_v2.0.a1
pyrus_communis	pycom04g08640
rosa_chinensis	RchiOBHm_Chr3g0470441
rosa_laevigata	RLG00000024267
rosa_multiflora	Rmu_sc0002383.1_g000006
rosa_roxburghii	Rroxscaffold_6G00410810
rosa_rugosa	Rorug03G0108900 Rorug03G0109000 Rorug03G0109100
rosa_samantha	Rh3AG159400 Rh3BG183800 Rh3CG174400 Rh3DG190900
rosa_wichuraiana	Rw3G015090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1406
AarI	CACCTGC	1 cut(s)	986
Acc36I	ACCTGC	2 cut(s)	637, 986
AccIII	TCCGGA	1 cut(s)	1357
AciI	CCGC	2 cut(s)	395, 1381
AclWI	GGATC	3 cut(s)	380, 822, 1242
AcoI	YGGCCR	1 cut(s)	434
AcsI	RAATTY	3 cut(s)	1040, 1352, 1437
AcuI	CTGAAG	2 cut(s)	76, 1134
AfaI	GTAC	2 cut(s)	25, 518
AfiI	CCNNNNNNNGG	2 cut(s)	139, 1480
AflII	CTTAAG	1 cut(s)	13
AjuI	GAANNNNNNNTTGG	4 cut(s)	446, 478, 1422, 1454
AluBI	AGCT	7 cut(s)	55, 96, 174, 359, 844, 1147, 1210
AluI	AGCT	7 cut(s)	55, 96, 174, 359, 844, 1147, 1210
Alw21I	GWGCWC	2 cut(s)	57, 1261
Alw44I	GTGCAC	1 cut(s)	1257
AlwI	GGATC	3 cut(s)	380, 822, 1242
Aor13HI	TCCGGA	1 cut(s)	1357
AoxI	GGCC	4 cut(s)	434, 489, 724, 824
ApaLI	GTGCAC	1 cut(s)	1257
ApoI	RAATTY	3 cut(s)	1040, 1352, 1437
Asp700I	GAANNNNTTC	1 cut(s)	112
AspLEI	GCGC	1 cut(s)	63
AspS9I	GGNCC	5 cut(s)	489, 824, 968, 1360, 1426
AsuC2I	CCSGG	1 cut(s)	1479
AsuHPI	GGTGA	4 cut(s)	395, 463, 1082, 1097
AvaII	GGWCC	3 cut(s)	968, 1360, 1426
BaeGI	GKGCMC	2 cut(s)	230, 1261
BalI	TGGCCA	1 cut(s)	436
BanII	GRGCYC	1 cut(s)	57
Bbv12I	GWGCWC	2 cut(s)	57, 1261
BbvCI	CCTCAGC	1 cut(s)	769
BccI	CCATC	7 cut(s)	238, 426, 641, 725, 1093, 1247, 1482
BclI	TGATCA	3 cut(s)	532, 1072, 1161
BcnI	CCSGG	1 cut(s)	1479
BfaI	CTAG	1 cut(s)	1262
BfmI	CTRYAG	1 cut(s)	520
BfrI	CTTAAG	1 cut(s)	13
BfuAI	ACCTGC	2 cut(s)	637, 986
BglI	GCCNNNNNGGC	1 cut(s)	1454
BisI	GCNGC	1 cut(s)	1381
BlsI	GCNGC	1 cut(s)	1382
BmcAI	AGTACT	1 cut(s)	518
Bme1390I	CCNGG	1 cut(s)	1479
Bme18I	GGWCC	3 cut(s)	968, 1360, 1426
BmgT120I	GGNCC	5 cut(s)	489, 824, 968, 1360, 1426
BmiI	GGNNCC	2 cut(s)	1029, 1398
BmrFI	CCNGG	1 cut(s)	1479
BmrI	ACTGGG	1 cut(s)	414
BmsI	GCATC	5 cut(s)	712, 889, 1069, 1144, 1260
BmuI	ACTGGG	1 cut(s)	414
Bpu10I	CCTNAGC	1 cut(s)	769
BpuEI	CTTGAG	1 cut(s)	1486
BpuMI	CCSGG	1 cut(s)	1479
BsaJI	CCNNGG	2 cut(s)	367, 1478
BsaWI	WCCGGW	2 cut(s)	1089, 1357
Bsc4I	CCNNNNNNNGG	2 cut(s)	139, 1480
Bse1I	ACTGG	3 cut(s)	297, 420, 1087
Bse3DI	GCAATG	1 cut(s)	774
BseAI	TCCGGA	1 cut(s)	1357
BseDI	CCNNGG	2 cut(s)	367, 1478
BseGI	GGATG	4 cut(s)	437, 793, 1219, 1474
BseLI	CCNNNNNNNGG	2 cut(s)	139, 1480
BseMI	GCAATG	1 cut(s)	774
BseMII	CTCAG	3 cut(s)	151, 783, 993
BseNI	ACTGG	3 cut(s)	297, 420, 1087
BseSI	GKGCMC	2 cut(s)	230, 1261
BsgI	GTGCAG	2 cut(s)	533, 959
BshFI	GGCC	4 cut(s)	436, 491, 726, 826
BsiHKAI	GWGCWC	2 cut(s)	57, 1261
BsiSI	CCGG	4 cut(s)	666, 1090, 1358, 1478
BslFI	GGGAC	1 cut(s)	1139
BslI	CCNNNNNNNGG	2 cut(s)	139, 1480
BsmFI	GGGAC	1 cut(s)	1139
BsnI	GGCC	4 cut(s)	436, 491, 726, 826
Bsp1286I	GDGCHC	3 cut(s)	57, 230, 1261
Bsp13I	TCCGGA	1 cut(s)	1357
Bsp143I	GATC	7 cut(s)	3, 385, 532, 814, 1072, 1161, 1247
BspACI	CCGC	2 cut(s)	395, 1381
BspANI	GGCC	4 cut(s)	436, 491, 726, 826
BspCNI	CTCAG	3 cut(s)	150, 782, 992
BspEI	TCCGGA	1 cut(s)	1357
BspHI	TCATGA	2 cut(s)	444, 1138
BspLI	GGNNCC	2 cut(s)	1029, 1398
BspMAI	CTGCAG	1 cut(s)	524
BspMI	ACCTGC	2 cut(s)	637, 986
BspPI	GGATC	3 cut(s)	380, 822, 1242
BspTI	CTTAAG	1 cut(s)	13
BsrDI	GCAATG	1 cut(s)	774
BsrI	ACTGG	3 cut(s)	297, 420, 1087
BssECI	CCNNGG	2 cut(s)	367, 1478
BssMI	GATC	7 cut(s)	3, 385, 532, 814, 1072, 1161, 1247
BssT1I	CCWWGG	1 cut(s)	367
Bst4CI	ACNGT	5 cut(s)	121, 571, 752, 808, 1229
Bst6I	CTCTTC	1 cut(s)	75
BstAFI	CTTAAG	1 cut(s)	13
BstAPI	GCANNNNNTGC	1 cut(s)	1001
BstC8I	GCNNGC	1 cut(s)	145
BstDEI	CTNAG	5 cut(s)	137, 769, 979, 1055, 1287
BstEII	GGTNACC	1 cut(s)	1085
BstF5I	GGATG	4 cut(s)	437, 793, 1219, 1474
BstHHI	GCGC	1 cut(s)	63
BstKTI	GATC	7 cut(s)	6, 388, 535, 817, 1075, 1164, 1250
BstMBI	GATC	7 cut(s)	3, 385, 532, 814, 1072, 1161, 1247
BstMWI	GCNNNNNNNGC	3 cut(s)	1001, 1216, 1454
BstPI	GGTNACC	1 cut(s)	1085
BstSCI	CCNGG	1 cut(s)	1477
BstSFI	CTRYAG	1 cut(s)	520
BstSLI	GKGCMC	2 cut(s)	230, 1261
BstX2I	RGATCY	1 cut(s)	1247
BstXI	CCANNNNNNTGG	2 cut(s)	781, 854
BstYI	RGATCY	1 cut(s)	1247
BsuRI	GGCC	4 cut(s)	436, 491, 726, 826
BtsCI	GGATG	4 cut(s)	437, 793, 1219, 1474
BtsI	GCAGTG	2 cut(s)	971, 1226
BtsIMutI	CAGTG	5 cut(s)	804, 971, 1080, 1122, 1226
BveI	ACCTGC	2 cut(s)	637, 986
Cac8I	GCNNGC	1 cut(s)	145
CciI	TCATGA	2 cut(s)	444, 1138
CfoI	GCGC	1 cut(s)	63
Cfr13I	GGNCC	5 cut(s)	489, 824, 968, 1360, 1426
Csp6I	GTAC	2 cut(s)	24, 517
CviAII	CATG	4 cut(s)	40, 445, 763, 1139
CviQI	GTAC	2 cut(s)	24, 517
DdeI	CTNAG	5 cut(s)	137, 769, 979, 1055, 1287
DpnI	GATC	7 cut(s)	5, 387, 534, 816, 1074, 1163, 1249
DpnII	GATC	7 cut(s)	3, 385, 532, 814, 1072, 1161, 1247
EaeI	YGGCCR	1 cut(s)	434
Eam1104I	CTCTTC	1 cut(s)	75
EarI	CTCTTC	1 cut(s)	75
Ecl136II	GAGCTC	1 cut(s)	55
Eco130I	CCWWGG	1 cut(s)	367
Eco24I	GRGCYC	1 cut(s)	57
Eco32I	GATATC	1 cut(s)	298
Eco47I	GGWCC	3 cut(s)	968, 1360, 1426
Eco53kI	GAGCTC	1 cut(s)	55
Eco57I	CTGAAG	2 cut(s)	76, 1134
Eco91I	GGTNACC	1 cut(s)	1085
EcoICRI	GAGCTC	1 cut(s)	55
EcoO109I	RGGNCCY	1 cut(s)	824
EcoO65I	GGTNACC	1 cut(s)	1085
EcoRI	GAATTC	1 cut(s)	1437
EcoRV	GATATC	1 cut(s)	298
EcoT14I	CCWWGG	1 cut(s)	367
EcoT22I	ATGCAT	1 cut(s)	583
EcoT38I	GRGCYC	1 cut(s)	57
ErhI	CCWWGG	1 cut(s)	367
FaeI	CATG	4 cut(s)	43, 448, 766, 1142
FaqI	GGGAC	1 cut(s)	1139
FatI	CATG	4 cut(s)	39, 444, 762, 1138
FbaI	TGATCA	3 cut(s)	532, 1072, 1161
Fnu4HI	GCNGC	1 cut(s)	1381
FokI	GGATG	4 cut(s)	444, 800, 1226, 1461
FriOI	GRGCYC	1 cut(s)	57
Fsp4HI	GCNGC	1 cut(s)	1381
FspBI	CTAG	1 cut(s)	1262
GlaI	GCGC	1 cut(s)	62
GluI	GCNGC	1 cut(s)	1381
HaeIII	GGCC	4 cut(s)	436, 491, 726, 826
HapII	CCGG	4 cut(s)	666, 1090, 1358, 1478
HhaI	GCGC	1 cut(s)	63
Hin1II	CATG	4 cut(s)	43, 448, 766, 1142
Hin6I	GCGC	1 cut(s)	61
HinP1I	GCGC	1 cut(s)	61
HinfI	GANTC	4 cut(s)	113, 378, 454, 793
HpaII	CCGG	4 cut(s)	666, 1090, 1358, 1478
HphI	GGTGA	4 cut(s)	395, 463, 1082, 1097
Hpy166II	GTNNAC	3 cut(s)	258, 971, 1259
Hpy188I	TCNGA	8 cut(s)	50, 310, 377, 607, 646, 888, 982, 1252
Hpy188III	TCNNGA	5 cut(s)	404, 445, 1031, 1139, 1358
Hpy8I	GTNNAC	3 cut(s)	258, 971, 1259
Hpy99I	CGWCG	1 cut(s)	927
HpyAV	CCTTC	4 cut(s)	100, 663, 837, 1109
HpyCH4III	ACNGT	5 cut(s)	121, 571, 752, 808, 1229
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1001, 1216, 1454
HpyF3I	CTNAG	5 cut(s)	137, 769, 979, 1055, 1287
Hsp92II	CATG	4 cut(s)	43, 448, 766, 1142
HspAI	GCGC	1 cut(s)	61
Kpn2I	TCCGGA	1 cut(s)	1357
Ksp22I	TGATCA	3 cut(s)	532, 1072, 1161
Kzo9I	GATC	7 cut(s)	3, 385, 532, 814, 1072, 1161, 1247
LmnI	GCTCC	5 cut(s)	166, 849, 1033, 1207, 1396
LweI	GCATC	5 cut(s)	712, 889, 1069, 1144, 1260
MaeI	CTAG	1 cut(s)	1262
MaeIII	GTNAC	2 cut(s)	985, 1085
MalI	GATC	7 cut(s)	5, 387, 534, 816, 1074, 1163, 1249
MboI	GATC	7 cut(s)	3, 385, 532, 814, 1072, 1161, 1247
MboII	GAAGA	7 cut(s)	92, 663, 678, 681, 923, 1049, 1058
MfeI	CAATTG	1 cut(s)	549
MflI	RGATCY	1 cut(s)	1247
MhlI	GDGCHC	3 cut(s)	57, 230, 1261
MlsI	TGGCCA	1 cut(s)	436
MluCI	AATT	8 cut(s)	410, 549, 598, 1040, 1233, 1320, 1352, 1437
MluNI	TGGCCA	1 cut(s)	436
MmeI	TCCRAC	1 cut(s)	409
MnlI	CCTC	9 cut(s)	76, 322, 441, 778, 805, 953, 1341, 1410, 1447
Mox20I	TGGCCA	1 cut(s)	436
Mph1103I	ATGCAT	1 cut(s)	583
MroI	TCCGGA	1 cut(s)	1357
MroXI	GAANNNNTTC	1 cut(s)	112
MscI	TGGCCA	1 cut(s)	436
MseI	TTAA	5 cut(s)	14, 99, 500, 594, 675
MslI	CAYNNNNRTG	3 cut(s)	449, 779, 981
Msp20I	TGGCCA	1 cut(s)	436
MspCI	CTTAAG	1 cut(s)	13
MspI	CCGG	4 cut(s)	666, 1090, 1358, 1478
MspR9I	CCNGG	1 cut(s)	1479
MunI	CAATTG	1 cut(s)	549
MwoI	GCNNNNNNNGC	3 cut(s)	1001, 1216, 1454
NciI	CCSGG	1 cut(s)	1479
NdeII	GATC	7 cut(s)	3, 385, 532, 814, 1072, 1161, 1247
NlaIII	CATG	4 cut(s)	43, 448, 766, 1142
NlaIV	GGNNCC	2 cut(s)	1029, 1398
NmuCI	GTSAC	2 cut(s)	985, 1085
NsiI	ATGCAT	1 cut(s)	583
PagI	TCATGA	2 cut(s)	444, 1138
PaqCI	CACCTGC	1 cut(s)	986
PdmI	GAANNNNTTC	1 cut(s)	112
PfeI	GAWTC	4 cut(s)	113, 378, 454, 793
PkrI	GCNGC	1 cut(s)	1382
PsiI	TTATAA	1 cut(s)	1406
Psp124BI	GAGCTC	1 cut(s)	57
PspEI	GGTNACC	1 cut(s)	1085
PspN4I	GGNNCC	2 cut(s)	1029, 1398
PspPI	GGNCC	5 cut(s)	489, 824, 968, 1360, 1426
PstI	CTGCAG	1 cut(s)	524
PsuI	RGATCY	1 cut(s)	1247
RsaI	GTAC	2 cut(s)	25, 518
RsaNI	GTAC	2 cut(s)	24, 517
RseI	CAYNNNNRTG	3 cut(s)	449, 779, 981
SacI	GAGCTC	1 cut(s)	57
SaqAI	TTAA	5 cut(s)	14, 99, 500, 594, 675
SatI	GCNGC	1 cut(s)	1381
Sau3AI	GATC	7 cut(s)	3, 385, 532, 814, 1072, 1161, 1247
Sau96I	GGNCC	5 cut(s)	489, 824, 968, 1360, 1426
ScaI	AGTACT	1 cut(s)	518
ScrFI	CCNGG	1 cut(s)	1479
SduI	GDGCHC	3 cut(s)	57, 230, 1261
SfaNI	GCATC	5 cut(s)	712, 889, 1069, 1144, 1260
SfcI	CTRYAG	1 cut(s)	520
SinI	GGWCC	3 cut(s)	968, 1360, 1426
SmiMI	CAYNNNNRTG	3 cut(s)	449, 779, 981
SmlI	CTYRAG	2 cut(s)	13, 1501
SmoI	CTYRAG	2 cut(s)	13, 1501
Sse9I	AATT	8 cut(s)	410, 549, 598, 1040, 1233, 1320, 1352, 1437
SsiI	CCGC	2 cut(s)	395, 1381
SspI	AATATT	2 cut(s)	76, 197
SspMI	CTAG	1 cut(s)	1262
SstI	GAGCTC	1 cut(s)	57
StyD4I	CCNGG	1 cut(s)	1477
StyI	CCWWGG	1 cut(s)	367
TaaI	ACNGT	5 cut(s)	121, 571, 752, 808, 1229
TaqI	TCGA	3 cut(s)	6, 222, 918
TaqII	GACCGA	1 cut(s)	1449
TasI	AATT	8 cut(s)	410, 549, 598, 1040, 1233, 1320, 1352, 1437
TatI	WGTACW	2 cut(s)	23, 516
TauI	GCSGC	1 cut(s)	1383
TfiI	GAWTC	4 cut(s)	113, 378, 454, 793
Tru1I	TTAA	5 cut(s)	14, 99, 500, 594, 675
Tru9I	TTAA	5 cut(s)	14, 99, 500, 594, 675
TscAI	CASTG	5 cut(s)	811, 978, 1087, 1129, 1226
TseFI	GTSAC	2 cut(s)	985, 1085
Tsp45I	GTSAC	2 cut(s)	985, 1085
TspDTI	ATGAA	6 cut(s)	433, 591, 734, 819, 1050, 1155
TspGWI	ACGGA	1 cut(s)	1409
TspRI	CASTG	5 cut(s)	811, 978, 1087, 1129, 1226
Vha464I	CTTAAG	1 cut(s)	13
VneI	GTGCAC	1 cut(s)	1257
VpaK11BI	GGWCC	3 cut(s)	968, 1360, 1426
XapI	RAATTY	3 cut(s)	1040, 1352, 1437
XmnI	GAANNNNTTC	1 cut(s)	112
XspI	CTAG	1 cut(s)	1262
ZrmI	AGTACT	1 cut(s)	518
Zsp2I	ATGCAT	1 cut(s)	583

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.