MD12G1111200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0012262
Physical Location & Seq
Forward (+)
17624891 .. 17628078
3188 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1111200.v1.1.491

Sequence Viewer

Length: 1098 bp
ATGATGAAACGACAGGATACTAATCTGCGGTCAGCATCTTTGCGCCGTGAAAATTCGCCGACAGAAAATCTCAACTCCAAGACCGTCGGCTGCATGTCCGGCATCTTCCACCTTTTCTCTAAACACCACTCTTCCCGCAAATTCCTCACTTTTGGGAAAAGGCAAGAGAGGAATGGGATTGGTTCCTCTTCTACAAGATCAAAATACTCGATAGCACTAATCAAAGGTTCCGCACCCGCCAAACTAGCTGAAACCAAACATGAAGACAAGTCCATTTATATTGGGAGATTCTCAAGCAATAACGTGCCAAGAAGCCCGACTCTGCCAGCAGAGATCCGGCGGTTTAGCTCCCTGGATTCGCCGGCTGAGAACTCCAGAACCCCACCTGCTCTAGTTGCTCGGCTGATGGGGTTGGAGCAGGCTCCGCGGATAATGTCAAAGATGCCAATGCAGGAGTCCATGTCCATCAACAAGAGGCAGCAGCTTCTGGGAGCACTAGAGAAATGTGATGAAGATTTGAAGGCCCTAAAGAAGATCATAGACAGTGTCCGGGCTGCTGAAACTCTCCGATCACCCGCCATGGTGAAGAGAATGGAGGCCGCCGTTGATGACAATAAGAAACGGTTCTACGGTGCAAATTTTAAGTGTATGGAGCTTACTAGTGAGCAGCCGAGTCCGGTGTCTGTGCTTGACGAGTTCACTCATTCGCCTTTCAGCAACCAGCACCACTACAGAAAACAAGTATTAAATTACGGACGAGCAGCGGCACAACCTCAACTAAGGAAGAGACCAGGAGAAGAGGACTTTTTGAATCCAAGTATGATTGACAAAATCACAACGGAATCAGTACTTTATAAGAGCCACAACGTAGAAGCACCATCGACACCATTATGGAATAGCAAGGCCATGGTAGAGAGTGTAGCAGAAGTGTGTAGGGACATTGCTTGGGGAGAGAGGAGAGAGATTGGAAGAATAGGGCTGGCTTTGCAGGACCACATTTGCAAAGACTTGATAGAAGAGATGGTCAAAGATATGGGATCATATACTTGTGTGTATTCACTGCCCCTTGAGGCATGTAAGAGAAGACTACGCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VARLMGL

Protein Analysis

366

Amino Acids

41.3

Weight (kDa)

9.6

Isoelectric Point (pI)

74.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012262)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G56810
fragaria_vesca FvH4_1g29470
malus_domestica MD12G1111200.v1.1
prunus_persica Prupe.7G023900_v2.0.a1 Prupe.7G023900_v2.0.a1 Prupe.7G023900_v2.0.a1 Prupe.7G023900_v2.0.a1
pyrus_communis pycom12g09350
rosa_chinensis RchiOBHm_Chr3g0495051
rosa_laevigata RLG00000022648
rosa_multiflora Rmu_sc0000340.1_g000035 Rmu_sc0004664.1_g000002
rosa_roxburghii Rroxscaffold_6G00390810
rosa_rugosa Rorug03G0269600
rosa_samantha Rh3AG318600 Rh3BG353900 Rh3CG351400 Rh3DG353000
rosa_wichuraiana Rw3G027790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 855
AarI CACCTGC 1 cut(s) 394
Acc36I ACCTGC 1 cut(s) 394
AccII CGCG 1 cut(s) 427
AclWI GGATC 2 cut(s) 328, 1045
AcsI RAATTY 3 cut(s) 52, 140, 637
AfaI GTAC 1 cut(s) 849
AgsI TTSAA 2 cut(s) 520, 811
AhlI ACTAGT 1 cut(s) 659
AjnI CCWGG 2 cut(s) 351, 790
AluBI AGCT 4 cut(s) 248, 348, 484, 655
AluI AGCT 4 cut(s) 248, 348, 484, 655
Alw21I GWGCWC 1 cut(s) 496
Alw26I GTCTC 1 cut(s) 781
AlwI GGATC 2 cut(s) 328, 1045
AlwNI CAGNNNCTG 1 cut(s) 487
AoxI GGCC 3 cut(s) 522, 597, 903
ApeKI GCWGC 6 cut(s) 90, 478, 481, 554, 667, 761
ApoI RAATTY 3 cut(s) 52, 140, 637
Asp700I GAANNNNTTC 1 cut(s) 623
AspLEI GCGC 1 cut(s) 45
AspS9I GGNCC 2 cut(s) 523, 991
AsuC2I CCSGG 1 cut(s) 551
AsuHPI GGTGA 2 cut(s) 564, 595
AvaII GGWCC 1 cut(s) 991
BbsI GAAGAC 2 cut(s) 270, 1090
Bbv12I GWGCWC 1 cut(s) 496
BbvI GCAGC 6 cut(s) 77, 490, 493, 541, 679, 773
BccI CCATC 4 cut(s) 400, 473, 886, 1015
BceAI ACGGC 2 cut(s) 30, 587
BciT130I CCWGG 2 cut(s) 353, 792
BciVI GTATCC 1 cut(s) 10
BcnI CCSGG 1 cut(s) 551
BcoDI GTCTC 1 cut(s) 781
BcuI ACTAGT 1 cut(s) 659
BfaI CTAG 4 cut(s) 245, 392, 497, 660
BfmI CTRYAG 1 cut(s) 730
BfuAI ACCTGC 1 cut(s) 394
BfuI GTATCC 1 cut(s) 10
BisI GCNGC 8 cut(s) 91, 479, 482, 555, 600, 668, 762, 765
BlsI GCNGC 8 cut(s) 92, 480, 483, 556, 601, 669, 763, 766
BmcAI AGTACT 1 cut(s) 849
Bme1390I CCNGG 3 cut(s) 353, 551, 792
Bme18I GGWCC 1 cut(s) 991
BmgT120I GGNCC 2 cut(s) 523, 991
BmiI GGNNCC 3 cut(s) 184, 229, 423
BmrFI CCNGG 3 cut(s) 353, 551, 792
BmsI GCATC 3 cut(s) 44, 111, 432
BpiI GAAGAC 2 cut(s) 270, 1090
BpmI CTGGAG 1 cut(s) 358
BpuEI CTTGAG 2 cut(s) 277, 1088
BpuMI CCSGG 1 cut(s) 551
BsaBI GATNNNNATC 1 cut(s) 21
BsaI GGTCTC 1 cut(s) 781
BsaJI CCNNGG 4 cut(s) 351, 425, 579, 906
BsaWI WCCGGW 1 cut(s) 676
BsaXI ACNNNNNCTCC 2 cut(s) 446, 476
Bse118I RCCGGY 1 cut(s) 361
Bse3DI GCAATG 1 cut(s) 939
Bse8I GATNNNNATC 1 cut(s) 21
BseBI CCWGG 2 cut(s) 353, 792
BseDI CCNNGG 4 cut(s) 351, 425, 579, 906
BseJI GATNNNNATC 1 cut(s) 21
BseMI GCAATG 1 cut(s) 939
BseMII CTCAG 1 cut(s) 357
BseRI GAGGAG 1 cut(s) 970
BseXI GCAGC 6 cut(s) 77, 490, 493, 541, 679, 773
Bsh1236I CGCG 1 cut(s) 427
BshFI GGCC 3 cut(s) 524, 599, 905
BsiHKAI GWGCWC 1 cut(s) 496
BsiSI CCGG 5 cut(s) 99, 337, 362, 550, 677
BslFI GGGAC 1 cut(s) 950
BsmAI GTCTC 1 cut(s) 781
BsmFI GGGAC 1 cut(s) 950
BsnI GGCC 3 cut(s) 524, 599, 905
Bso31I GGTCTC 1 cut(s) 781
Bsp1286I GDGCHC 1 cut(s) 496
Bsp143I GATC 5 cut(s) 197, 333, 534, 569, 1037
Bsp19I CCATGG 2 cut(s) 579, 906
BspANI GGCC 3 cut(s) 524, 599, 905
BspCNI CTCAG 1 cut(s) 358
BspFNI CGCG 1 cut(s) 427
BspLI GGNNCC 3 cut(s) 184, 229, 423
BspMI ACCTGC 1 cut(s) 394
BspPI GGATC 2 cut(s) 328, 1045
BspTNI GGTCTC 1 cut(s) 781
BsrDI GCAATG 1 cut(s) 939
BsrFI RCCGGY 1 cut(s) 361
BssAI RCCGGY 1 cut(s) 361
BssECI CCNNGG 4 cut(s) 351, 425, 579, 906
BssMI GATC 5 cut(s) 197, 333, 534, 569, 1037
BssT1I CCWWGG 2 cut(s) 579, 906
Bst2UI CCWGG 2 cut(s) 353, 792
Bst4CI ACNGT 4 cut(s) 85, 545, 624, 632
Bst6I CTCTTC 6 cut(s) 136, 193, 581, 779, 792, 1011
BstC8I GCNNGC 4 cut(s) 327, 363, 420, 981
BstDEI CTNAG 2 cut(s) 366, 779
BstDSI CCRYGG 3 cut(s) 425, 579, 906
BstFNI CGCG 1 cut(s) 427
BstHHI GCGC 1 cut(s) 45
BstKTI GATC 5 cut(s) 200, 336, 537, 572, 1040
BstMAI GTCTC 1 cut(s) 781
BstMBI GATC 5 cut(s) 197, 333, 534, 569, 1037
BstMWI GCNNNNNNNGC 5 cut(s) 99, 245, 395, 424, 985
BstNI CCWGG 2 cut(s) 353, 792
BstNSI RCATGY 2 cut(s) 97, 1077
BstSCI CCNGG 3 cut(s) 351, 549, 790
BstSFI CTRYAG 1 cut(s) 730
BstUI CGCG 1 cut(s) 427
BstV1I GCAGC 6 cut(s) 77, 490, 493, 541, 679, 773
BstV2I GAAGAC 2 cut(s) 270, 1090
BstX2I RGATCY 1 cut(s) 333
BstYI RGATCY 1 cut(s) 333
BsuI GTATCC 1 cut(s) 10
BsuRI GGCC 3 cut(s) 524, 599, 905
BtgI CCRYGG 3 cut(s) 425, 579, 906
BtsI GCAGTG 1 cut(s) 1058
BtsIMutI CAGTG 2 cut(s) 550, 1058
BveI ACCTGC 1 cut(s) 394
Cac8I GCNNGC 4 cut(s) 327, 363, 420, 981
CaiI CAGNNNCTG 1 cut(s) 487
CfoI GCGC 1 cut(s) 45
Cfr10I RCCGGY 1 cut(s) 361
Cfr13I GGNCC 2 cut(s) 523, 991
Cfr42I CCGCGG 1 cut(s) 428
Csp6I GTAC 1 cut(s) 848
CviAII CATG 6 cut(s) 94, 260, 460, 580, 907, 1074
CviQI GTAC 1 cut(s) 848
DdeI CTNAG 2 cut(s) 366, 779
DpnI GATC 5 cut(s) 199, 335, 536, 571, 1039
DpnII GATC 5 cut(s) 197, 333, 534, 569, 1037
Eam1104I CTCTTC 6 cut(s) 136, 193, 581, 779, 792, 1011
EarI CTCTTC 6 cut(s) 136, 193, 581, 779, 792, 1011
Eco130I CCWWGG 2 cut(s) 579, 906
Eco31I GGTCTC 1 cut(s) 781
Eco47I GGWCC 1 cut(s) 991
EcoO109I RGGNCCY 1 cut(s) 523
EcoRII CCWGG 2 cut(s) 351, 790
EcoT14I CCWWGG 2 cut(s) 579, 906
ErhI CCWWGG 2 cut(s) 579, 906
FaeI CATG 6 cut(s) 97, 263, 463, 583, 910, 1077
FaqI GGGAC 1 cut(s) 950
FatI CATG 6 cut(s) 93, 259, 459, 579, 906, 1073
FauI CCCGC 3 cut(s) 143, 244, 583
Fnu4HI GCNGC 8 cut(s) 91, 479, 482, 555, 600, 668, 762, 765
Fsp4HI GCNGC 8 cut(s) 91, 479, 482, 555, 600, 668, 762, 765
FspBI CTAG 4 cut(s) 245, 392, 497, 660
GlaI GCGC 1 cut(s) 44
GluI GCNGC 8 cut(s) 91, 479, 482, 555, 600, 668, 762, 765
GsuI CTGGAG 1 cut(s) 358
HaeIII GGCC 3 cut(s) 524, 599, 905
HapII CCGG 5 cut(s) 99, 337, 362, 550, 677
HhaI GCGC 1 cut(s) 45
Hin1II CATG 6 cut(s) 97, 263, 463, 583, 910, 1077
Hin6I GCGC 1 cut(s) 43
HinP1I GCGC 1 cut(s) 43
HinfI GANTC 7 cut(s) 288, 319, 356, 455, 673, 811, 842
HpaII CCGG 5 cut(s) 99, 337, 362, 550, 677
HphI GGTGA 2 cut(s) 564, 595
Hpy166II GTNNAC 1 cut(s) 699
Hpy188I TCNGA 1 cut(s) 569
Hpy188III TCNNGA 1 cut(s) 375
Hpy8I GTNNAC 1 cut(s) 699
Hpy99I CGWCG 1 cut(s) 89
HpyAV CCTTC 1 cut(s) 514
HpyCH4III ACNGT 4 cut(s) 85, 545, 624, 632
HpyCH4IV ACGT 2 cut(s) 303, 867
HpyCH4V TGCA 5 cut(s) 93, 451, 635, 988, 1002
HpyF10VI GCNNNNNNNGC 5 cut(s) 99, 245, 395, 424, 985
HpyF3I CTNAG 2 cut(s) 366, 779
HpySE526I ACGT 2 cut(s) 303, 867
Hsp92II CATG 6 cut(s) 97, 263, 463, 583, 910, 1077
HspAI GCGC 1 cut(s) 43
KroI GCCGGC 1 cut(s) 361
KroNI GCCGGC 1 cut(s) 363
KspI CCGCGG 1 cut(s) 428
Kzo9I GATC 5 cut(s) 197, 333, 534, 569, 1037
LmnI GCTCC 5 cut(s) 353, 415, 427, 491, 652
Lsp1109I GCAGC 6 cut(s) 77, 490, 493, 541, 679, 773
LweI GCATC 3 cut(s) 44, 111, 432
MaeI CTAG 4 cut(s) 245, 392, 497, 660
MaeII ACGT 2 cut(s) 303, 867
MalI GATC 5 cut(s) 199, 335, 536, 571, 1039
MboI GATC 5 cut(s) 197, 333, 534, 569, 1037
MflI RGATCY 1 cut(s) 333
MhlI GDGCHC 1 cut(s) 496
MluCI AATT 4 cut(s) 52, 140, 637, 748
MlyI GAGTC 3 cut(s) 313, 464, 682
MmeI TCCRAC 1 cut(s) 393
MnlI CCTC 9 cut(s) 155, 162, 196, 468, 589, 783, 793, 948, 1063
MroNI GCCGGC 1 cut(s) 361
MroXI GAANNNNTTC 1 cut(s) 623
MseI TTAA 2 cut(s) 642, 746
MslI CAYNNNNRTG 1 cut(s) 889
MspA1I CMGCKG 2 cut(s) 427, 764
MspI CCGG 5 cut(s) 99, 337, 362, 550, 677
MspR9I CCNGG 3 cut(s) 353, 551, 792
MvaI CCWGG 2 cut(s) 353, 792
MvnI CGCG 1 cut(s) 427
MwoI GCNNNNNNNGC 5 cut(s) 99, 245, 395, 424, 985
NaeI GCCGGC 1 cut(s) 363
NciI CCSGG 1 cut(s) 551
NcoI CCATGG 2 cut(s) 579, 906
NdeII GATC 5 cut(s) 197, 333, 534, 569, 1037
NgoMIV GCCGGC 1 cut(s) 361
NlaIII CATG 6 cut(s) 97, 263, 463, 583, 910, 1077
NlaIV GGNNCC 3 cut(s) 184, 229, 423
NmeAIII GCCGAG 2 cut(s) 379, 696
NspI RCATGY 2 cut(s) 97, 1077
PaqCI CACCTGC 1 cut(s) 394
PdiI GCCGGC 1 cut(s) 363
PdmI GAANNNNTTC 1 cut(s) 623
PfeI GAWTC 4 cut(s) 288, 356, 811, 842
PflFI GACNNNGTC 1 cut(s) 545
PkrI GCNGC 8 cut(s) 92, 480, 483, 556, 601, 669, 763, 766
PleI GAGTC 3 cut(s) 313, 463, 681
PpsI GAGTC 3 cut(s) 313, 463, 681
PsiI TTATAA 1 cut(s) 855
Psp6I CCWGG 2 cut(s) 351, 790
PspGI CCWGG 2 cut(s) 351, 790
PspN4I GGNNCC 3 cut(s) 184, 229, 423
PspPI GGNCC 2 cut(s) 523, 991
PstNI CAGNNNCTG 1 cut(s) 487
PsuI RGATCY 1 cut(s) 333
PsyI GACNNNGTC 1 cut(s) 545
RsaI GTAC 1 cut(s) 849
RsaNI GTAC 1 cut(s) 848
RseI CAYNNNNRTG 1 cut(s) 889
SacII CCGCGG 1 cut(s) 428
SaqAI TTAA 2 cut(s) 642, 746
SatI GCNGC 8 cut(s) 91, 479, 482, 555, 600, 668, 762, 765
Sau3AI GATC 5 cut(s) 197, 333, 534, 569, 1037
Sau96I GGNCC 2 cut(s) 523, 991
ScaI AGTACT 1 cut(s) 849
SchI GAGTC 3 cut(s) 313, 464, 682
ScrFI CCNGG 3 cut(s) 353, 551, 792
SduI GDGCHC 1 cut(s) 496
SfaNI GCATC 3 cut(s) 44, 111, 432
SfcI CTRYAG 1 cut(s) 730
Sfr303I CCGCGG 1 cut(s) 428
SgrBI CCGCGG 1 cut(s) 428
SinI GGWCC 1 cut(s) 991
SmiMI CAYNNNNRTG 1 cut(s) 889
SmlI CTYRAG 2 cut(s) 292, 1067
SmoI CTYRAG 2 cut(s) 292, 1067
SpeI ACTAGT 1 cut(s) 659
Sse9I AATT 4 cut(s) 52, 140, 637, 748
SspMI CTAG 4 cut(s) 245, 392, 497, 660
StyD4I CCNGG 3 cut(s) 351, 549, 790
StyI CCWWGG 2 cut(s) 579, 906
TaaI ACNGT 4 cut(s) 85, 545, 624, 632
TaiI ACGT 2 cut(s) 306, 870
TaqI TCGA 2 cut(s) 209, 881
TasI AATT 4 cut(s) 52, 140, 637, 748
TatI WGTACW 1 cut(s) 847
TauI GCSGC 2 cut(s) 602, 767
TfiI GAWTC 4 cut(s) 288, 356, 811, 842
Tru1I TTAA 2 cut(s) 642, 746
Tru9I TTAA 2 cut(s) 642, 746
TscAI CASTG 2 cut(s) 550, 1065
TseI GCWGC 6 cut(s) 90, 478, 481, 554, 667, 761
TspDTI ATGAA 3 cut(s) 20, 276, 525
TspGWI ACGGA 2 cut(s) 768, 854
TspRI CASTG 2 cut(s) 550, 1065
Tth111I GACNNNGTC 1 cut(s) 545
VpaK11BI GGWCC 1 cut(s) 991
XapI RAATTY 3 cut(s) 52, 140, 637
XceI RCATGY 2 cut(s) 97, 1077
XmnI GAANNNNTTC 1 cut(s) 623
XspI CTAG 4 cut(s) 245, 392, 497, 660
ZrmI AGTACT 1 cut(s) 849
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.