MD12G1114600.v1.1

Belongs to the 14-3-3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0016942
Physical Location & Seq
Forward (+)
18268415 .. 18268624
210 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1114600.v1.1.491

Sequence Viewer

Length: 210 bp
ATGGCTTCCGTCGTCCCCGATAACCTCACCAGGGAGCAGTACGTGTACCTGGCGAAGCTCTCTGAGCAAGCAGAGCGCTATGAGGAGATGGTCCAGTTCATGGAGAAATTGGTCGTTGGTTCGACTCCGGCCGTCGAGCTGACTGTCGAGGAGCGCAACCTTCTTTCCATCACGTACAAGAACGTGATCGGCTCCTTCCGCGCCGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

70

Amino Acids

7.86

Weight (kDa)

4.8

Isoelectric Point (pI)

26.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 17 - 69 4.5e-14 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 100
AccII CGCG 1 cut(s) 201
AciI CCGC 2 cut(s) 199, 204
AcoI YGGCCR 1 cut(s) 129
AfaI GTAC 3 cut(s) 41, 47, 176
AfeI AGCGCT 1 cut(s) 77
AfiI CCNNNNNNNGG 2 cut(s) 31, 100
AflIII ACRYGT 1 cut(s) 42
AjnI CCWGG 2 cut(s) 29, 48
AluBI AGCT 2 cut(s) 58, 139
AluI AGCT 2 cut(s) 58, 139
Aor51HI AGCGCT 1 cut(s) 77
AoxI GGCC 1 cut(s) 129
AspLEI GCGC 3 cut(s) 78, 156, 203
AspS9I GGNCC 1 cut(s) 91
AsuHPI GGTGA 1 cut(s) 19
AvaII GGWCC 1 cut(s) 91
BccI CCATC 2 cut(s) 82, 176
BceAI ACGGC 1 cut(s) 116
BciT130I CCWGG 2 cut(s) 31, 50
BfoI RGCGCY 1 cut(s) 79
BisI GCNGC 1 cut(s) 204
BlsI GCNGC 1 cut(s) 205
Bme1390I CCNGG 2 cut(s) 31, 50
Bme18I GGWCC 1 cut(s) 91
BmgT120I GGNCC 1 cut(s) 91
BmiI GGNNCC 1 cut(s) 193
BmrFI CCNGG 2 cut(s) 31, 50
BsaAI YACGTR 2 cut(s) 43, 174
BsaJI CCNNGG 1 cut(s) 30
Bsc4I CCNNNNNNNGG 2 cut(s) 31, 100
Bse1I ACTGG 1 cut(s) 94
BseBI CCWGG 2 cut(s) 31, 50
BseDI CCNNGG 1 cut(s) 30
BseLI CCNNNNNNNGG 2 cut(s) 31, 100
BseMII CTCAG 1 cut(s) 54
BseNI ACTGG 1 cut(s) 94
BseRI GAGGAG 2 cut(s) 98, 164
BseX3I CGGCCG 1 cut(s) 129
Bsh1236I CGCG 1 cut(s) 201
Bsh1285I CGRYCG 1 cut(s) 132
BshFI GGCC 1 cut(s) 131
BsiEI CGRYCG 1 cut(s) 132
BsiSI CCGG 1 cut(s) 128
BslI CCNNNNNNNGG 2 cut(s) 31, 100
BsnI GGCC 1 cut(s) 131
Bsp143I GATC 1 cut(s) 186
BspACI CCGC 2 cut(s) 199, 204
BspANI GGCC 1 cut(s) 131
BspCNI CTCAG 1 cut(s) 55
BspFNI CGCG 1 cut(s) 201
BspLI GGNNCC 1 cut(s) 193
BsrI ACTGG 1 cut(s) 94
BssECI CCNNGG 1 cut(s) 30
BssMI GATC 1 cut(s) 186
Bst2UI CCWGG 2 cut(s) 31, 50
Bst4CI ACNGT 1 cut(s) 145
BstBAI YACGTR 2 cut(s) 43, 174
BstC8I GCNNGC 1 cut(s) 69
BstDEI CTNAG 2 cut(s) 63, 207
BstFNI CGCG 1 cut(s) 201
BstH2I RGCGCY 1 cut(s) 79
BstHHI GCGC 3 cut(s) 78, 156, 203
BstKTI GATC 1 cut(s) 189
BstMBI GATC 1 cut(s) 186
BstMCI CGRYCG 1 cut(s) 132
BstMWI GCNNNNNNNGC 3 cut(s) 64, 73, 198
BstNI CCWGG 2 cut(s) 31, 50
BstSCI CCNGG 2 cut(s) 29, 48
BstUI CGCG 1 cut(s) 201
BstZI CGGCCG 1 cut(s) 129
BsuRI GGCC 1 cut(s) 131
Cac8I GCNNGC 1 cut(s) 69
CfoI GCGC 3 cut(s) 78, 156, 203
Cfr13I GGNCC 1 cut(s) 91
Csp6I GTAC 3 cut(s) 40, 46, 175
CviAII CATG 1 cut(s) 100
CviJI RGCY 5 cut(s) 5, 58, 131, 139, 192
CviKI_1 RGCY 5 cut(s) 5, 58, 131, 139, 192
CviQI GTAC 3 cut(s) 40, 46, 175
DdeI CTNAG 2 cut(s) 63, 207
DpnI GATC 1 cut(s) 188
DpnII GATC 1 cut(s) 186
EaeI YGGCCR 1 cut(s) 129
EagI CGGCCG 1 cut(s) 129
EclXI CGGCCG 1 cut(s) 129
Eco47I GGWCC 1 cut(s) 91
Eco47III AGCGCT 1 cut(s) 77
Eco52I CGGCCG 1 cut(s) 129
EcoRII CCWGG 2 cut(s) 29, 48
FaeI CATG 1 cut(s) 103
FaiI YATR 2 cut(s) 81, 101
FatI CATG 1 cut(s) 99
Fnu4HI GCNGC 1 cut(s) 204
Fsp4HI GCNGC 1 cut(s) 204
GlaI GCGC 3 cut(s) 77, 155, 202
GluI GCNGC 1 cut(s) 204
HaeII RGCGCY 1 cut(s) 79
HaeIII GGCC 1 cut(s) 131
HapII CCGG 1 cut(s) 128
HhaI GCGC 3 cut(s) 78, 156, 203
Hin1II CATG 1 cut(s) 103
Hin6I GCGC 3 cut(s) 76, 154, 201
HinP1I GCGC 3 cut(s) 76, 154, 201
HinfI GANTC 1 cut(s) 124
HpaII CCGG 1 cut(s) 128
HphI GGTGA 1 cut(s) 19
Hpy166II GTNNAC 1 cut(s) 46
Hpy188I TCNGA 1 cut(s) 64
Hpy8I GTNNAC 1 cut(s) 46
Hpy99I CGWCG 2 cut(s) 14, 137
HpyAV CCTTC 2 cut(s) 170, 205
HpyCH4III ACNGT 1 cut(s) 145
HpyCH4IV ACGT 3 cut(s) 42, 173, 183
HpyF10VI GCNNNNNNNGC 3 cut(s) 64, 73, 198
HpyF3I CTNAG 2 cut(s) 63, 207
HpySE526I ACGT 3 cut(s) 42, 173, 183
Hsp92II CATG 1 cut(s) 103
HspAI GCGC 3 cut(s) 76, 154, 201
Kzo9I GATC 1 cut(s) 186
LmnI GCTCC 3 cut(s) 34, 151, 197
LpnPI CCDG 6 cut(s) 16, 35, 43, 62, 107, 141
MaeII ACGT 3 cut(s) 42, 173, 183
MalI GATC 1 cut(s) 188
MboI GATC 1 cut(s) 186
MluCI AATT 1 cut(s) 107
MlyI GAGTC 1 cut(s) 118
MnlI CCTC 3 cut(s) 35, 76, 142
MspI CCGG 1 cut(s) 128
MspR9I CCNGG 2 cut(s) 31, 50
MvaI CCWGG 2 cut(s) 31, 50
MvnI CGCG 1 cut(s) 201
MwoI GCNNNNNNNGC 3 cut(s) 64, 73, 198
NdeII GATC 1 cut(s) 186
NlaIII CATG 1 cut(s) 103
NlaIV GGNNCC 1 cut(s) 193
PflMI CCANNNNNTGG 1 cut(s) 100
PkrI GCNGC 1 cut(s) 205
PleI GAGTC 1 cut(s) 118
PpsI GAGTC 1 cut(s) 118
Ppu21I YACGTR 2 cut(s) 43, 174
Psp6I CCWGG 2 cut(s) 29, 48
PspGI CCWGG 2 cut(s) 29, 48
PspN4I GGNNCC 1 cut(s) 193
PspPI GGNCC 1 cut(s) 91
RsaI GTAC 3 cut(s) 41, 47, 176
RsaNI GTAC 3 cut(s) 40, 46, 175
SatI GCNGC 1 cut(s) 204
Sau3AI GATC 1 cut(s) 186
Sau96I GGNCC 1 cut(s) 91
SchI GAGTC 1 cut(s) 118
ScrFI CCNGG 2 cut(s) 31, 50
SetI ASST 8 cut(s) 27, 45, 51, 60, 141, 162, 176, 186
SinI GGWCC 1 cut(s) 91
Sse9I AATT 1 cut(s) 107
SsiI CCGC 2 cut(s) 199, 204
StyD4I CCNGG 2 cut(s) 29, 48
TaaI ACNGT 1 cut(s) 145
TaiI ACGT 3 cut(s) 45, 176, 186
TaqI TCGA 3 cut(s) 122, 135, 147
TasI AATT 1 cut(s) 107
TauI GCSGC 1 cut(s) 206
TspDTI ATGAA 1 cut(s) 88
Van91I CCANNNNNTGG 1 cut(s) 100
VpaK11BI GGWCC 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.