Rroxscaffold_1G00027990

Belongs to the 14-3-3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0016942
Physical Location & Seq
Reverse (-)
35687411 .. 35691856
4446 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00027990.1

Sequence Viewer

Length: 327 bp
ATGTTCATAAGAGTATCCAAGCTTATAAGAGACCAGCACGTCTACTTGGCTAAGCTCACCGAGCAGGCGGAGCGCTACGAGGAGATGGGTCCACCACGGCGTCGGAGCTCACCGTCGAGGAGCGCATCGAGCGAAGGAGGACGTGCGCTTGTCAAGGAGTACAGATCCAAGGTCGAGGCCGAGCTCTTTAAGGTCCGCGCGTCGGAGCTCACCGTCGAGGAGCGCATCGAGCGTAAGGAGGAGGGCCGCAAGAACGAGGAGGACGTGGCGCTCGTCAAGGAGTACAGATCCAAGGTCGAGGCCGAGCTCTCTCGAGGTCCGCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

108

Amino Acids

12.61

Weight (kDa)

9.47

Isoelectric Point (pI)

64.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 26
AasI GACNNNNNNGTC 1 cut(s) 38
AccI GTMKAC 1 cut(s) 42
AccII CGCG 3 cut(s) 198, 200, 322
AciI CCGC 4 cut(s) 68, 196, 247, 320
AclWI GGATC 2 cut(s) 159, 282
AcyI GRCGYC 1 cut(s) 100
AfaI GTAC 2 cut(s) 161, 284
AfeI AGCGCT 1 cut(s) 74
AfiI CCNNNNNNNGG 1 cut(s) 202
AjiI CACGTC 3 cut(s) 40, 143, 265
AluBI AGCT 6 cut(s) 22, 55, 108, 184, 208, 307
AluI AGCT 6 cut(s) 22, 55, 108, 184, 208, 307
Alw21I GWGCWC 4 cut(s) 110, 186, 210, 309
Alw26I GTCTC 1 cut(s) 24
AlwI GGATC 2 cut(s) 159, 282
Ama87I CYCGRG 1 cut(s) 312
Aor51HI AGCGCT 1 cut(s) 74
AoxI GGCC 3 cut(s) 177, 244, 300
AspLEI GCGC 7 cut(s) 75, 125, 148, 200, 225, 271, 324
AspS9I GGNCC 4 cut(s) 89, 193, 244, 317
AsuHPI GGTGA 3 cut(s) 49, 102, 202
AvaI CYCGRG 1 cut(s) 312
AvaII GGWCC 3 cut(s) 89, 193, 317
BanII GRGCYC 4 cut(s) 110, 186, 210, 309
Bbv12I GWGCWC 4 cut(s) 110, 186, 210, 309
BccI CCATC 1 cut(s) 79
BceAI ACGGC 1 cut(s) 113
BciVI GTATCC 1 cut(s) 25
BcoDI GTCTC 1 cut(s) 24
BfoI RGCGCY 2 cut(s) 76, 272
BfuI GTATCC 1 cut(s) 25
BisI GCNGC 1 cut(s) 247
BlpI GCTNAGC 1 cut(s) 51
BlsI GCNGC 1 cut(s) 248
Bme18I GGWCC 3 cut(s) 89, 193, 317
BmeT110I CYCGRG 1 cut(s) 312
BmgBI CACGTC 3 cut(s) 40, 143, 265
BmgT120I GGNCC 4 cut(s) 89, 193, 244, 317
BmiI GGNNCC 1 cut(s) 90
BmsI GCATC 2 cut(s) 134, 234
Bpu1102I GCTNAGC 1 cut(s) 51
BsaHI GRCGYC 1 cut(s) 100
BsaI GGTCTC 1 cut(s) 24
BsaJI CCNNGG 3 cut(s) 95, 168, 291
Bsc4I CCNNNNNNNGG 1 cut(s) 202
BseDI CCNNGG 3 cut(s) 95, 168, 291
BseLI CCNNNNNNNGG 1 cut(s) 202
BseRI GAGGAG 5 cut(s) 95, 133, 233, 254, 272
Bsh1236I CGCG 3 cut(s) 198, 200, 322
BshFI GGCC 3 cut(s) 179, 246, 302
BsiHKAI GWGCWC 4 cut(s) 110, 186, 210, 309
BsiHKCI CYCGRG 1 cut(s) 312
BslI CCNNNNNNNGG 1 cut(s) 202
BsmAI GTCTC 1 cut(s) 24
BsnI GGCC 3 cut(s) 179, 246, 302
Bso31I GGTCTC 1 cut(s) 24
BsoBI CYCGRG 1 cut(s) 312
Bsp1286I GDGCHC 4 cut(s) 110, 186, 210, 309
Bsp143I GATC 2 cut(s) 164, 287
Bsp1720I GCTNAGC 1 cut(s) 51
BspACI CCGC 4 cut(s) 68, 196, 247, 320
BspANI GGCC 3 cut(s) 179, 246, 302
BspFNI CGCG 3 cut(s) 198, 200, 322
BspLI GGNNCC 1 cut(s) 90
BspPI GGATC 2 cut(s) 159, 282
BspTNI GGTCTC 1 cut(s) 24
BssECI CCNNGG 3 cut(s) 95, 168, 291
BssMI GATC 2 cut(s) 164, 287
BssNI GRCGYC 1 cut(s) 100
BssT1I CCWWGG 2 cut(s) 168, 291
Bst4CI ACNGT 2 cut(s) 114, 214
BstACI GRCGYC 1 cut(s) 100
BstC8I GCNNGC 1 cut(s) 66
BstDEI CTNAG 1 cut(s) 51
BstDSI CCRYGG 1 cut(s) 95
BstFNI CGCG 3 cut(s) 198, 200, 322
BstH2I RGCGCY 2 cut(s) 76, 272
BstHHI GCGC 7 cut(s) 75, 125, 148, 200, 225, 271, 324
BstKTI GATC 2 cut(s) 167, 290
BstMAI GTCTC 1 cut(s) 24
BstMBI GATC 2 cut(s) 164, 287
BstMWI GCNNNNNNNGC 4 cut(s) 61, 70, 129, 229
BstUI CGCG 3 cut(s) 198, 200, 322
BstX2I RGATCY 2 cut(s) 164, 287
BstYI RGATCY 2 cut(s) 164, 287
BsuI GTATCC 1 cut(s) 25
BsuRI GGCC 3 cut(s) 179, 246, 302
BtgI CCRYGG 1 cut(s) 95
BtrI CACGTC 3 cut(s) 40, 143, 265
Cac8I GCNNGC 1 cut(s) 66
CfoI GCGC 7 cut(s) 75, 125, 148, 200, 225, 271, 324
Cfr13I GGNCC 4 cut(s) 89, 193, 244, 317
CseI GACGC 2 cut(s) 89, 189
Csp6I GTAC 2 cut(s) 160, 283
CviQI GTAC 2 cut(s) 160, 283
DdeI CTNAG 1 cut(s) 51
DpnI GATC 2 cut(s) 166, 289
DpnII GATC 2 cut(s) 164, 287
DrdI GACNNNNNNGTC 1 cut(s) 38
DseDI GACNNNNNNGTC 1 cut(s) 38
EciI GGCGGA 1 cut(s) 83
Ecl136II GAGCTC 4 cut(s) 108, 184, 208, 307
Eco130I CCWWGG 2 cut(s) 168, 291
Eco24I GRGCYC 4 cut(s) 110, 186, 210, 309
Eco31I GGTCTC 1 cut(s) 24
Eco47I GGWCC 3 cut(s) 89, 193, 317
Eco47III AGCGCT 1 cut(s) 74
Eco53kI GAGCTC 4 cut(s) 108, 184, 208, 307
Eco88I CYCGRG 1 cut(s) 312
EcoICRI GAGCTC 4 cut(s) 108, 184, 208, 307
EcoT14I CCWWGG 2 cut(s) 168, 291
EcoT38I GRGCYC 4 cut(s) 110, 186, 210, 309
ErhI CCWWGG 2 cut(s) 168, 291
FaiI YATR 2 cut(s) 8, 26
FblI GTMKAC 1 cut(s) 42
Fnu4HI GCNGC 1 cut(s) 247
FriOI GRGCYC 4 cut(s) 110, 186, 210, 309
Fsp4HI GCNGC 1 cut(s) 247
GlaI GCGC 7 cut(s) 74, 124, 147, 199, 224, 270, 323
GluI GCNGC 1 cut(s) 247
HaeII RGCGCY 2 cut(s) 76, 272
HaeIII GGCC 3 cut(s) 179, 246, 302
HgaI GACGC 2 cut(s) 89, 189
HhaI GCGC 7 cut(s) 75, 125, 148, 200, 225, 271, 324
Hin1I GRCGYC 1 cut(s) 100
Hin6I GCGC 7 cut(s) 73, 123, 146, 198, 223, 269, 322
HinP1I GCGC 7 cut(s) 73, 123, 146, 198, 223, 269, 322
HindIII AAGCTT 1 cut(s) 20
HphI GGTGA 3 cut(s) 49, 102, 202
Hpy166II GTNNAC 2 cut(s) 43, 92
Hpy188I TCNGA 2 cut(s) 105, 205
Hpy188III TCNNGA 1 cut(s) 312
Hpy8I GTNNAC 2 cut(s) 43, 92
Hpy99I CGWCG 4 cut(s) 105, 118, 205, 218
HpyAV CCTTC 1 cut(s) 128
HpyCH4III ACNGT 2 cut(s) 114, 214
HpyCH4IV ACGT 3 cut(s) 39, 142, 264
HpyF10VI GCNNNNNNNGC 4 cut(s) 61, 70, 129, 229
HpyF3I CTNAG 1 cut(s) 51
HpySE526I ACGT 3 cut(s) 39, 142, 264
Hsp92I GRCGYC 1 cut(s) 100
HspAI GCGC 7 cut(s) 73, 123, 146, 198, 223, 269, 322
Kzo9I GATC 2 cut(s) 164, 287
LmnI GCTCC 5 cut(s) 70, 105, 120, 205, 220
LpnPI CCDG 2 cut(s) 47, 50
LweI GCATC 2 cut(s) 134, 234
MaeII ACGT 3 cut(s) 39, 142, 264
MalI GATC 2 cut(s) 166, 289
MboI GATC 2 cut(s) 164, 287
MflI RGATCY 2 cut(s) 164, 287
MhlI GDGCHC 4 cut(s) 110, 186, 210, 309
MmeI TCCRAC 2 cut(s) 83, 183
MseI TTAA 1 cut(s) 189
MvnI CGCG 3 cut(s) 198, 200, 322
MwoI GCNNNNNNNGC 4 cut(s) 61, 70, 129, 229
NdeII GATC 2 cut(s) 164, 287
NlaIV GGNNCC 1 cut(s) 90
NmeAIII GCCGAG 1 cut(s) 205
PaeR7I CTCGAG 1 cut(s) 312
PcsI WCGNNNNNNNCGW 2 cut(s) 261, 270
PkrI GCNGC 1 cut(s) 248
PsiI TTATAA 1 cut(s) 26
Psp124BI GAGCTC 4 cut(s) 110, 186, 210, 309
PspN4I GGNNCC 1 cut(s) 90
PspPI GGNCC 4 cut(s) 89, 193, 244, 317
PsuI RGATCY 2 cut(s) 164, 287
RsaI GTAC 2 cut(s) 161, 284
RsaNI GTAC 2 cut(s) 160, 283
SacI GAGCTC 4 cut(s) 110, 186, 210, 309
SaqAI TTAA 1 cut(s) 189
SatI GCNGC 1 cut(s) 247
Sau3AI GATC 2 cut(s) 164, 287
Sau96I GGNCC 4 cut(s) 89, 193, 244, 317
SduI GDGCHC 4 cut(s) 110, 186, 210, 309
SfaNI GCATC 2 cut(s) 134, 234
Sfr274I CTCGAG 1 cut(s) 312
SinI GGWCC 3 cut(s) 89, 193, 317
SlaI CTCGAG 1 cut(s) 312
SmlI CTYRAG 1 cut(s) 312
SmoI CTYRAG 1 cut(s) 312
SsiI CCGC 4 cut(s) 68, 196, 247, 320
SstI GAGCTC 4 cut(s) 110, 186, 210, 309
StyI CCWWGG 2 cut(s) 168, 291
TaaI ACNGT 2 cut(s) 114, 214
TaiI ACGT 3 cut(s) 42, 145, 267
TaqI TCGA 7 cut(s) 116, 128, 174, 216, 228, 297, 313
TatI WGTACW 2 cut(s) 159, 282
TauI GCSGC 1 cut(s) 249
Tru1I TTAA 1 cut(s) 189
Tru9I TTAA 1 cut(s) 189
VpaK11BI GGWCC 3 cut(s) 89, 193, 317
XhoI CTCGAG 1 cut(s) 312
XmiI GTMKAC 1 cut(s) 42
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.