RLG00000019449

Belongs to the 14-3-3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0016942
Physical Location & Seq
Forward (+)
52137696 .. 52138262
567 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019449

Sequence Viewer

Length: 459 bp
ATGGCGTCCTCCATCCCCGATAACCTAACCAGAGACCAGCACGTCTACTTGGCCAAGCTCACCGAGCAGGCGGAGCGCTACGAGGAGATGGGTCCACCAAGGCGTCGGAGTGCACCGTCGAGGAGCGCATCGAGCAGAAGGAGGAGGGCCGCAAGAACGAGGAGGACGTGGTGCTCGTCAAGGAGTACAGATCCAAGGTCGAGGCCGAGCTCTCTGAGCTACGAAGGAGTAGATTCTTCGGCTGATTTGTTGAGAATAAGCGAAGATGTGGCGGGGTGGTGGTGGAGCTCCGGCGGGGTCGACGACAACATCACTCTCTCCCTCCCCCCGGCGAAAGTTCACCAACGAGCCCACCTGAGGCGGTTTTCCGGTGAGATCAAGTACGGCTTCACAACTTCACAGCTTCACAGAAGGCAGAAGGGTGCCCAAATCAATTTCATCACCTGCCCCGGCGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

153

Amino Acids

17.25

Weight (kDa)

10.56

Isoelectric Point (pI)

83.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 452
AasI GACNNNNNNGTC 1 cut(s) 41
Acc36I ACCTGC 1 cut(s) 452
AccB1I GGYRCC 1 cut(s) 422
AccI GTMKAC 2 cut(s) 45, 300
AciI CCGC 5 cut(s) 71, 150, 272, 294, 361
AclWI GGATC 1 cut(s) 185
AcoI YGGCCR 1 cut(s) 51
AcyI GRCGYC 2 cut(s) 5, 103
AfaI GTAC 2 cut(s) 187, 383
AfeI AGCGCT 1 cut(s) 77
AfiI CCNNNNNNNGG 2 cut(s) 328, 357
AjiI CACGTC 2 cut(s) 43, 168
AluBI AGCT 5 cut(s) 58, 210, 219, 288, 403
AluI AGCT 5 cut(s) 58, 210, 219, 288, 403
Alw21I GWGCWC 4 cut(s) 115, 176, 212, 290
Alw26I GTCTC 1 cut(s) 27
Alw44I GTGCAC 1 cut(s) 111
AlwI GGATC 1 cut(s) 185
Aor51HI AGCGCT 1 cut(s) 77
AoxI GGCC 3 cut(s) 51, 147, 203
ApaLI GTGCAC 1 cut(s) 111
AspLEI GCGC 2 cut(s) 78, 128
AspS9I GGNCC 2 cut(s) 92, 147
AsuC2I CCSGG 2 cut(s) 329, 450
AsuHPI GGTGA 4 cut(s) 52, 332, 383, 433
AvaII GGWCC 1 cut(s) 92
AxyI CCTNAGG 1 cut(s) 356
BaeGI GKGCMC 2 cut(s) 115, 427
BalI TGGCCA 1 cut(s) 53
BanI GGYRCC 1 cut(s) 422
BanII GRGCYC 3 cut(s) 212, 290, 352
Bbv12I GWGCWC 4 cut(s) 115, 176, 212, 290
BccI CCATC 2 cut(s) 20, 82
BceAI ACGGC 1 cut(s) 400
BcnI CCSGG 2 cut(s) 329, 450
BcoDI GTCTC 1 cut(s) 27
BfaI CTAG 1 cut(s) 457
BfoI RGCGCY 1 cut(s) 79
BfuAI ACCTGC 1 cut(s) 452
BisI GCNGC 1 cut(s) 150
BlsI GCNGC 1 cut(s) 151
Bme1390I CCNGG 2 cut(s) 329, 450
Bme18I GGWCC 1 cut(s) 92
BmgBI CACGTC 2 cut(s) 43, 168
BmgT120I GGNCC 2 cut(s) 92, 147
BmiI GGNNCC 2 cut(s) 93, 424
BmrFI CCNGG 2 cut(s) 329, 450
BmsI GCATC 1 cut(s) 137
BpuMI CCSGG 2 cut(s) 329, 450
BsaHI GRCGYC 2 cut(s) 5, 103
BsaI GGTCTC 1 cut(s) 27
BsaJI CCNNGG 4 cut(s) 98, 194, 327, 448
BsaWI WCCGGW 1 cut(s) 368
Bsc4I CCNNNNNNNGG 2 cut(s) 328, 357
Bse21I CCTNAGG 1 cut(s) 356
BseDI CCNNGG 4 cut(s) 98, 194, 327, 448
BseGI GGATG 1 cut(s) 12
BseLI CCNNNNNNNGG 2 cut(s) 328, 357
BseMII CTCAG 2 cut(s) 206, 347
BseRI GAGGAG 4 cut(s) 98, 136, 157, 175
BseSI GKGCMC 2 cut(s) 115, 427
BshFI GGCC 3 cut(s) 53, 149, 205
BshNI GGYRCC 1 cut(s) 422
BsiHKAI GWGCWC 4 cut(s) 115, 176, 212, 290
BsiSI CCGG 4 cut(s) 291, 329, 369, 450
BslI CCNNNNNNNGG 2 cut(s) 328, 357
BsmAI GTCTC 1 cut(s) 27
BsnI GGCC 3 cut(s) 53, 149, 205
Bso31I GGTCTC 1 cut(s) 27
Bsp1286I GDGCHC 6 cut(s) 115, 176, 212, 290, 352, 427
Bsp143I GATC 2 cut(s) 190, 375
BspACI CCGC 5 cut(s) 71, 150, 272, 294, 361
BspANI GGCC 3 cut(s) 53, 149, 205
BspCNI CTCAG 2 cut(s) 207, 348
BspLI GGNNCC 2 cut(s) 93, 424
BspMI ACCTGC 1 cut(s) 452
BspPI GGATC 1 cut(s) 185
BspT107I GGYRCC 1 cut(s) 422
BspTNI GGTCTC 1 cut(s) 27
BssECI CCNNGG 4 cut(s) 98, 194, 327, 448
BssMI GATC 2 cut(s) 190, 375
BssNI GRCGYC 2 cut(s) 5, 103
BssT1I CCWWGG 2 cut(s) 98, 194
Bst4CI ACNGT 1 cut(s) 117
BstACI GRCGYC 2 cut(s) 5, 103
BstC8I GCNNGC 1 cut(s) 69
BstDEI CTNAG 2 cut(s) 215, 356
BstF5I GGATG 1 cut(s) 12
BstH2I RGCGCY 1 cut(s) 79
BstHHI GCGC 2 cut(s) 78, 128
BstKTI GATC 2 cut(s) 193, 378
BstMAI GTCTC 1 cut(s) 27
BstMBI GATC 2 cut(s) 190, 375
BstMWI GCNNNNNNNGC 4 cut(s) 64, 73, 132, 216
BstSCI CCNGG 2 cut(s) 327, 448
BstSLI GKGCMC 2 cut(s) 115, 427
BstX2I RGATCY 1 cut(s) 190
BstYI RGATCY 1 cut(s) 190
Bsu36I CCTNAGG 1 cut(s) 356
BsuRI GGCC 3 cut(s) 53, 149, 205
BtrI CACGTC 2 cut(s) 43, 168
BtsCI GGATG 1 cut(s) 12
BveI ACCTGC 1 cut(s) 452
Cac8I GCNNGC 1 cut(s) 69
CfoI GCGC 2 cut(s) 78, 128
Cfr13I GGNCC 2 cut(s) 92, 147
CseI GACGC 1 cut(s) 92
Csp6I GTAC 2 cut(s) 186, 382
CviQI GTAC 2 cut(s) 186, 382
DdeI CTNAG 2 cut(s) 215, 356
DpnI GATC 2 cut(s) 192, 377
DpnII GATC 2 cut(s) 190, 375
DrdI GACNNNNNNGTC 1 cut(s) 41
DseDI GACNNNNNNGTC 1 cut(s) 41
EaeI YGGCCR 1 cut(s) 51
EciI GGCGGA 1 cut(s) 86
Ecl136II GAGCTC 2 cut(s) 210, 288
Eco130I CCWWGG 2 cut(s) 98, 194
Eco24I GRGCYC 3 cut(s) 212, 290, 352
Eco31I GGTCTC 1 cut(s) 27
Eco47I GGWCC 1 cut(s) 92
Eco47III AGCGCT 1 cut(s) 77
Eco53kI GAGCTC 2 cut(s) 210, 288
Eco81I CCTNAGG 1 cut(s) 356
EcoICRI GAGCTC 2 cut(s) 210, 288
EcoT14I CCWWGG 2 cut(s) 98, 194
EcoT38I GRGCYC 3 cut(s) 212, 290, 352
ErhI CCWWGG 2 cut(s) 98, 194
FalI AAGNNNNNCTT 2 cut(s) 371, 403
FauI CCCGC 2 cut(s) 265, 287
FblI GTMKAC 2 cut(s) 45, 300
Fnu4HI GCNGC 1 cut(s) 150
FriOI GRGCYC 3 cut(s) 212, 290, 352
Fsp4HI GCNGC 1 cut(s) 150
FspBI CTAG 1 cut(s) 457
GlaI GCGC 2 cut(s) 77, 127
GluI GCNGC 1 cut(s) 150
HaeII RGCGCY 1 cut(s) 79
HaeIII GGCC 3 cut(s) 53, 149, 205
HapII CCGG 4 cut(s) 291, 329, 369, 450
HgaI GACGC 1 cut(s) 92
HhaI GCGC 2 cut(s) 78, 128
Hin1I GRCGYC 2 cut(s) 5, 103
Hin6I GCGC 2 cut(s) 76, 126
HinP1I GCGC 2 cut(s) 76, 126
HincII GTYRAC 1 cut(s) 301
HindII GTYRAC 1 cut(s) 301
HinfI GANTC 1 cut(s) 233
HpaII CCGG 4 cut(s) 291, 329, 369, 450
HphI GGTGA 4 cut(s) 52, 332, 383, 433
Hpy166II GTNNAC 5 cut(s) 46, 95, 113, 301, 340
Hpy188I TCNGA 2 cut(s) 108, 216
Hpy8I GTNNAC 5 cut(s) 46, 95, 113, 301, 340
Hpy99I CGWCG 3 cut(s) 108, 121, 305
HpyAV CCTTC 4 cut(s) 132, 218, 405, 412
HpyCH4III ACNGT 1 cut(s) 117
HpyCH4IV ACGT 2 cut(s) 42, 167
HpyCH4V TGCA 1 cut(s) 113
HpyF10VI GCNNNNNNNGC 4 cut(s) 64, 73, 132, 216
HpyF3I CTNAG 2 cut(s) 215, 356
HpySE526I ACGT 2 cut(s) 42, 167
Hsp92I GRCGYC 2 cut(s) 5, 103
HspAI GCGC 2 cut(s) 76, 126
Kzo9I GATC 2 cut(s) 190, 375
LmnI GCTCC 4 cut(s) 73, 123, 285, 293
LpnPI CCDG 7 cut(s) 43, 50, 53, 304, 342, 368, 382
LweI GCATC 1 cut(s) 137
MaeI CTAG 1 cut(s) 457
MaeII ACGT 2 cut(s) 42, 167
MalI GATC 2 cut(s) 192, 377
MboI GATC 2 cut(s) 190, 375
MboII GAAGA 2 cut(s) 228, 275
MflI RGATCY 1 cut(s) 190
MhlI GDGCHC 6 cut(s) 115, 176, 212, 290, 352, 427
MlsI TGGCCA 1 cut(s) 53
MluCI AATT 1 cut(s) 433
MluNI TGGCCA 1 cut(s) 53
MmeI TCCRAC 1 cut(s) 86
Mox20I TGGCCA 1 cut(s) 53
MscI TGGCCA 1 cut(s) 53
Msp20I TGGCCA 1 cut(s) 53
MspI CCGG 4 cut(s) 291, 329, 369, 450
MspR9I CCNGG 2 cut(s) 329, 450
MwoI GCNNNNNNNGC 4 cut(s) 64, 73, 132, 216
NciI CCSGG 2 cut(s) 329, 450
NdeII GATC 2 cut(s) 190, 375
NlaIV GGNNCC 2 cut(s) 93, 424
NmeAIII GCCGAG 1 cut(s) 231
PaqCI CACCTGC 1 cut(s) 452
PcsI WCGNNNNNNNCGW 2 cut(s) 164, 173
PfeI GAWTC 1 cut(s) 233
PkrI GCNGC 1 cut(s) 151
Psp124BI GAGCTC 2 cut(s) 212, 290
PspN4I GGNNCC 2 cut(s) 93, 424
PspPI GGNCC 2 cut(s) 92, 147
PsuI RGATCY 1 cut(s) 190
RsaI GTAC 2 cut(s) 187, 383
RsaNI GTAC 2 cut(s) 186, 382
SacI GAGCTC 2 cut(s) 212, 290
SalI GTCGAC 1 cut(s) 299
SatI GCNGC 1 cut(s) 150
Sau3AI GATC 2 cut(s) 190, 375
Sau96I GGNCC 2 cut(s) 92, 147
ScrFI CCNGG 2 cut(s) 329, 450
SduI GDGCHC 6 cut(s) 115, 176, 212, 290, 352, 427
SfaNI GCATC 1 cut(s) 137
SinI GGWCC 1 cut(s) 92
Sse9I AATT 1 cut(s) 433
SsiI CCGC 5 cut(s) 71, 150, 272, 294, 361
SspMI CTAG 1 cut(s) 457
SstI GAGCTC 2 cut(s) 212, 290
StyD4I CCNGG 2 cut(s) 327, 448
StyI CCWWGG 2 cut(s) 98, 194
TaaI ACNGT 1 cut(s) 117
TaiI ACGT 2 cut(s) 45, 170
TaqI TCGA 4 cut(s) 119, 131, 200, 300
TasI AATT 1 cut(s) 433
TatI WGTACW 1 cut(s) 185
TauI GCSGC 1 cut(s) 152
TfiI GAWTC 1 cut(s) 233
TspDTI ATGAA 1 cut(s) 427
VneI GTGCAC 1 cut(s) 111
VpaK11BI GGWCC 1 cut(s) 92
XmiI GTMKAC 2 cut(s) 45, 300
XspI CTAG 1 cut(s) 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.