Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0011577

Physical Location & Seq

Genomic Coordinates Reverse (-)

20125299 .. 20128901

Gene Structure 3603 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1125900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1389 bp

ATGCTAGATCTCAACCTCGGGTTCGTCTCCCACGACGTCGTTCCCGATTCCCACCACAAGCTTCTCTCCACCACCCCGATCGAAAGCTCCGAAAGCTTCAACTCCTCCTCCAACCTCACCCTCGCCGGCGACGACGACGATAACTCCGCTACTCTCAACTTCCTTGCTCCGAACGACGACGTATCCGCTGATCACTACGACGGTCGGAGGACCATCCAGCTCTTCCCGCTCGCCCAATCCGTACGCTCTTCTTCGTCGTCGTCGTCGGCTTCTAAGAAGCAGTGGCTGGGACTATCGTCCGGTTCCAGATTGGAGCTGGAGCCGATTTACTATGCTCCTGCGAAGCAATGGATTGCACCTCCGCAGCAAGTGAAGAAGAGCCGGAGAGGGCCCCGGTCCCGGAGCTCGCAGTATCGGGGCGTTACGTTTTATCGAAGAACCGGGAGATGGGAATCCCATATTTGGGATTGCGGGAAACAAGTGTACTTGGGAGGATTTGACACTGCCCAATCTGCTGCTAGGGCGTATGATCGAGCTGCGATCAAATTCCGTGGAACTGAGGCTGATATTAACTTCAGTGTCAGTGATTATGAGGATGATATTAAGCAGATGAGTAATTTTACAAAGGAAGAATTTGTGCATATTCTGCGCCGCCAGAGTACCGGGTTCTCTAGAGGAAGGTCGAAATTCAGGGGAGTCACATTGCACAAATGTGGCCGCTGGGAAGCTCGTATGGGCCAGTTTATGGGCAGGAAGGCATATGACCAGGCTGCCATTGAGTGCAATGGAAGAGAAGCAGTCACCAACTTCGATTCAAGCTCATATGATGGGGTGTTAATGTCTGAGGCCAATAACAAAGGCAGCCACCAAAATCTTGATCTGAACTTGGGAATTGCTCCCCCCTTCGTTTCTGAAATCCAAAAGGAGAACATCAATTTGAGCAGCTTTCCTTTTCAGCTAGGCTGTGACGGCATTCCTATTCACACAAGAGCAAGGAATGAGAACTCCGTTCCAGCACCTATGAGGTCTCAACTTTCTCATGGCTCAATGGTGGCGTCTGAGGTGCATCCTATAATGAGCAACATGAATTCCAGTTTCTTTCCCATTCATATGGAAAGAGCTATGGAGAAGAGTATGGATGTTAACTCATTTCCCAGTTGGGCTTGGCAACTCCAAGACCTGAATGGCGGGCCAACTTCCATGCCACTCTTATCTACTGCAGCATCATCAGGATTCCCTTCTTCAACGGCCACTTCATCGTCATCAGCTGTCACTCAACTTCATTTCCCTAACATGGCGATTCTCCGCCACCATTTTTCACCGTCATTCACCAACAACATCCCCCATTTTGCAGAGGCTGAAAACCAGAAGCAGGTCACGATCGGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003002 GO:0003006 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0007389 GO:0008150 GO:0009653 GO:0009791 GO:0009888 GO:0009889 GO:0009908 GO:0010073 GO:0010093 GO:0010154 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0035670 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048316 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048444 GO:0048449 GO:0048467 GO:0048481 GO:0048507 GO:0048608 GO:0048646 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0090701 GO:0099402 GO:0140110 GO:1903506 GO:1905392 GO:1905393 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AP2

Protein Analysis

463

Amino Acids

51.26

Weight (kDa)

7.29

Isoelectric Point (pI)

67.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AP2	PF00847	137 - 185	6.7e-10	AP2 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011577)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g15180 FvH4_6g15180
malus_domestica	MD04G1105200.v1.1 MD12G1125900.v1.1
prunus_persica	Prupe.6G242400_v2.0.a1 Prupe.6G242400_v2.0.a1
pyrus_communis	pycom04g10050 pycom12g12410
rosa_chinensis	RchiOBHm_Chr3g0468481 RchiOBHm_Chr3g0468491
rosa_laevigata	RLG00000024425
rosa_roxburghii	Rroxscaffold_6G00412720
rosa_rugosa	Rorug03G0095700 Rorug03G0095700 Rorug03G0095700
rosa_samantha	Rh3AG143600 Rh3AG144100 Rh3BG166100 Rh3DG165700
rosa_wichuraiana	Rw3G013600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	39
Acc36I	ACCTGC	1 cut(s)	1363
AccB7I	CCANNNNNTGG	1 cut(s)	745
AccBSI	CCGCTC	1 cut(s)	229
AciI	CCGC	9 cut(s)	147, 186, 227, 362, 471, 652, 718, 1188, 1306
AcoI	YGGCCR	2 cut(s)	715, 1248
AcsI	RAATTY	4 cut(s)	545, 632, 686, 1087
AcuI	CTGAAG	1 cut(s)	559
AcyI	GRCGYC	2 cut(s)	36, 1055
AfaI	GTAC	3 cut(s)	243, 485, 661
AfiI	CCNNNNNNNGG	7 cut(s)	399, 447, 462, 463, 745, 1294, 1372
AgsI	TTSAA	3 cut(s)	100, 816, 1245
AjnI	CCWGG	1 cut(s)	765
AleI	CACNNNNGTG	1 cut(s)	711
Alw21I	GWGCWC	1 cut(s)	407
Alw26I	GTCTC	2 cut(s)	31, 1032
AlwNI	CAGNNNCTG	2 cut(s)	286, 1358
Ama87I	CYCGRG	1 cut(s)	17
AoxI	GGCC	6 cut(s)	389, 715, 736, 846, 1190, 1248
ApaI	GGGCCC	1 cut(s)	393
ApeKI	GCWGC	7 cut(s)	364, 515, 536, 770, 861, 942, 1220
ApoI	RAATTY	4 cut(s)	545, 632, 686, 1087
AspLEI	GCGC	1 cut(s)	651
AspS9I	GGNCC	6 cut(s)	210, 389, 390, 396, 736, 1190
AsuC2I	CCSGG	4 cut(s)	394, 400, 442, 664
AsuHPI	GGTGA	4 cut(s)	109, 793, 1311, 1321
AvaI	CYCGRG	1 cut(s)	17
AvaII	GGWCC	2 cut(s)	210, 396
BaeGI	GKGCMC	1 cut(s)	393
BanII	GRGCYC	2 cut(s)	393, 407
Bbv12I	GWGCWC	1 cut(s)	407
BbvI	GCAGC	7 cut(s)	376, 502, 523, 757, 873, 954, 1232
BccI	CCATC	3 cut(s)	221, 441, 821
BceAI	ACGGC	2 cut(s)	985, 1263
BciT130I	CCWGG	1 cut(s)	767
BciVI	GTATCC	1 cut(s)	193
BclI	TGATCA	1 cut(s)	190
BcnI	CCSGG	4 cut(s)	394, 400, 442, 664
BcoDI	GTCTC	2 cut(s)	31, 1032
BfaI	CTAG	4 cut(s)	5, 519, 672, 959
BfmI	CTRYAG	1 cut(s)	1218
BfuAI	ACCTGC	1 cut(s)	1363
BfuI	GTATCC	1 cut(s)	193
BglII	AGATCT	1 cut(s)	7
BisI	GCNGC	9 cut(s)	365, 516, 537, 652, 718, 771, 862, 943, 1221
BlsI	GCNGC	9 cut(s)	366, 517, 538, 653, 719, 772, 863, 944, 1222
Bme1390I	CCNGG	5 cut(s)	394, 400, 442, 664, 767
Bme18I	GGWCC	2 cut(s)	210, 396
BmeT110I	CYCGRG	1 cut(s)	17
BmgT120I	GGNCC	6 cut(s)	210, 389, 390, 396, 736, 1190
BmiI	GGNNCC	5 cut(s)	304, 321, 391, 392, 398
BmrFI	CCNGG	5 cut(s)	394, 400, 442, 664, 767
BmrI	ACTGGG	1 cut(s)	1149
BmsI	GCATC	2 cut(s)	1075, 1232
BmuI	ACTGGG	1 cut(s)	1149
BoxI	GACNNNNGTC	1 cut(s)	295
BpmI	CTGGAG	1 cut(s)	338
BpuMI	CCSGG	4 cut(s)	394, 400, 442, 664
BsaBI	GATNNNNATC	1 cut(s)	451
BsaHI	GRCGYC	2 cut(s)	36, 1055
BsaI	GGTCTC	1 cut(s)	1032
BsaJI	CCNNGG	3 cut(s)	16, 392, 550
BsaWI	WCCGGW	1 cut(s)	299
BsaXI	ACNNNNNCTCC	4 cut(s)	92, 122, 128, 158
Bsc4I	CCNNNNNNNGG	7 cut(s)	399, 447, 462, 463, 745, 1294, 1372
Bse118I	RCCGGY	1 cut(s)	125
Bse1I	ACTGG	3 cut(s)	739, 1092, 1155
Bse3DI	GCAATG	3 cut(s)	353, 701, 790
Bse8I	GATNNNNATC	1 cut(s)	451
BseBI	CCWGG	1 cut(s)	767
BseDI	CCNNGG	3 cut(s)	16, 392, 550
BseGI	GGATG	5 cut(s)	213, 601, 1066, 1144, 1338
BseJI	GATNNNNATC	1 cut(s)	451
BseLI	CCNNNNNNNGG	7 cut(s)	399, 447, 462, 463, 745, 1294, 1372
BseMI	GCAATG	3 cut(s)	353, 701, 790
BseMII	CTCAG	3 cut(s)	549, 834, 1050
BseNI	ACTGG	3 cut(s)	739, 1092, 1155
BseRI	GAGGAG	2 cut(s)	94, 97
BseSI	GKGCMC	1 cut(s)	393
BseXI	GCAGC	7 cut(s)	376, 502, 523, 757, 873, 954, 1232
BseYI	CCCAGC	2 cut(s)	286, 720
Bsh1285I	CGRYCG	3 cut(s)	81, 205, 1383
BshFI	GGCC	6 cut(s)	391, 717, 738, 848, 1192, 1250
BsiEI	CGRYCG	3 cut(s)	81, 205, 1383
BsiHKAI	GWGCWC	1 cut(s)	407
BsiHKCI	CYCGRG	1 cut(s)	17
BsiSI	CCGG	7 cut(s)	126, 300, 382, 394, 400, 441, 663
BsiWI	CGTACG	1 cut(s)	241
BslFI	GGGAC	2 cut(s)	303, 382
BslI	CCNNNNNNNGG	7 cut(s)	399, 447, 462, 463, 745, 1294, 1372
BsmAI	GTCTC	2 cut(s)	31, 1032
BsmBI	CGTCTC	1 cut(s)	31
BsmFI	GGGAC	2 cut(s)	303, 382
BsmI	GAATGC	1 cut(s)	972
BsnI	GGCC	6 cut(s)	391, 717, 738, 848, 1192, 1250
Bso31I	GGTCTC	1 cut(s)	1032
BsoBI	CYCGRG	1 cut(s)	17
Bsp120I	GGGCCC	1 cut(s)	389
Bsp1286I	GDGCHC	2 cut(s)	393, 407
Bsp143I	GATC	7 cut(s)	7, 78, 190, 529, 540, 877, 1380
BspACI	CCGC	9 cut(s)	147, 186, 227, 362, 471, 652, 718, 1188, 1306
BspANI	GGCC	6 cut(s)	391, 717, 738, 848, 1192, 1250
BspCNI	CTCAG	3 cut(s)	550, 835, 1051
BspLI	GGNNCC	5 cut(s)	304, 321, 391, 392, 398
BspMAI	CTGCAG	1 cut(s)	1222
BspMI	ACCTGC	1 cut(s)	1363
BspQI	GCTCTTC	3 cut(s)	227, 253, 371
BspTNI	GGTCTC	1 cut(s)	1032
BsrBI	CCGCTC	1 cut(s)	229
BsrDI	GCAATG	3 cut(s)	353, 701, 790
BsrFI	RCCGGY	1 cut(s)	125
BsrI	ACTGG	3 cut(s)	739, 1092, 1155
BssAI	RCCGGY	1 cut(s)	125
BssECI	CCNNGG	3 cut(s)	16, 392, 550
BssMI	GATC	7 cut(s)	7, 78, 190, 529, 540, 877, 1380
BssNI	GRCGYC	2 cut(s)	36, 1055
Bst2UI	CCWGG	1 cut(s)	767
Bst4CI	ACNGT	2 cut(s)	203, 1323
Bst6I	CTCTTC	5 cut(s)	227, 253, 371, 784, 1124
BstACI	GRCGYC	2 cut(s)	36, 1055
BstAPI	GCANNNNNTGC	1 cut(s)	646
BstC8I	GCNNGC	4 cut(s)	127, 231, 407, 1190
BstDEI	CTNAG	4 cut(s)	273, 558, 843, 1059
BstDSI	CCRYGG	1 cut(s)	550
BstF5I	GGATG	5 cut(s)	213, 601, 1066, 1144, 1338
BstHHI	GCGC	1 cut(s)	651
BstKTI	GATC	7 cut(s)	10, 81, 193, 532, 543, 880, 1383
BstMAI	GTCTC	2 cut(s)	31, 1032
BstMBI	GATC	7 cut(s)	7, 78, 190, 529, 540, 877, 1380
BstMCI	CGRYCG	3 cut(s)	81, 205, 1383
BstMWI	GCNNNNNNNGC	6 cut(s)	93, 226, 512, 521, 646, 969
BstNI	CCWGG	1 cut(s)	767
BstPAI	GACNNNNGTC	1 cut(s)	295
BstSCI	CCNGG	5 cut(s)	392, 398, 440, 662, 765
BstSFI	CTRYAG	1 cut(s)	1218
BstSLI	GKGCMC	1 cut(s)	393
BstV1I	GCAGC	7 cut(s)	376, 502, 523, 757, 873, 954, 1232
BstX2I	RGATCY	1 cut(s)	7
BstXI	CCANNNNNNTGG	1 cut(s)	1111
BstYI	RGATCY	1 cut(s)	7
BsuI	GTATCC	1 cut(s)	193
BsuRI	GGCC	6 cut(s)	391, 717, 738, 848, 1192, 1250
BtgI	CCRYGG	1 cut(s)	550
BtsCI	GGATG	5 cut(s)	213, 601, 1066, 1144, 1338
BtsI	GCAGTG	2 cut(s)	287, 501
BtsIMutI	CAGTG	4 cut(s)	287, 501, 583, 589
BveI	ACCTGC	1 cut(s)	1363
Cac8I	GCNNGC	4 cut(s)	127, 231, 407, 1190
CaiI	CAGNNNCTG	2 cut(s)	286, 1358
CfoI	GCGC	1 cut(s)	651
Cfr10I	RCCGGY	1 cut(s)	125
Cfr13I	GGNCC	6 cut(s)	210, 389, 390, 396, 736, 1190
CseI	GACGC	1 cut(s)	1044
Csp6I	GTAC	3 cut(s)	242, 484, 660
CspCI	CAANNNNNGTGG	2 cut(s)	532, 567
CviAII	CATG	4 cut(s)	1040, 1084, 1201, 1294
CviQI	GTAC	3 cut(s)	242, 484, 660
DdeI	CTNAG	4 cut(s)	273, 558, 843, 1059
DpnI	GATC	7 cut(s)	9, 80, 192, 531, 542, 879, 1382
DpnII	GATC	7 cut(s)	7, 78, 190, 529, 540, 877, 1380
EaeI	YGGCCR	2 cut(s)	715, 1248
Eam1104I	CTCTTC	5 cut(s)	227, 253, 371, 784, 1124
EarI	CTCTTC	5 cut(s)	227, 253, 371, 784, 1124
EciI	GGCGGA	1 cut(s)	1295
Ecl136II	GAGCTC	1 cut(s)	405
Eco24I	GRGCYC	2 cut(s)	393, 407
Eco31I	GGTCTC	1 cut(s)	1032
Eco47I	GGWCC	2 cut(s)	210, 396
Eco53kI	GAGCTC	1 cut(s)	405
Eco57I	CTGAAG	1 cut(s)	559
Eco88I	CYCGRG	1 cut(s)	17
EcoICRI	GAGCTC	1 cut(s)	405
EcoO109I	RGGNCCY	2 cut(s)	389, 390
EcoRI	GAATTC	1 cut(s)	1087
EcoRII	CCWGG	1 cut(s)	765
EcoT38I	GRGCYC	2 cut(s)	393, 407
Esp3I	CGTCTC	1 cut(s)	31
FaeI	CATG	4 cut(s)	1043, 1087, 1204, 1297
FaqI	GGGAC	2 cut(s)	303, 382
FatI	CATG	4 cut(s)	1039, 1083, 1200, 1293
FauI	CCCGC	3 cut(s)	234, 464, 1181
FauNDI	CATATG	3 cut(s)	760, 823, 1110
FbaI	TGATCA	1 cut(s)	190
Fnu4HI	GCNGC	9 cut(s)	365, 516, 537, 652, 718, 771, 862, 943, 1221
FokI	GGATG	5 cut(s)	200, 608, 1053, 1151, 1325
FriOI	GRGCYC	2 cut(s)	393, 407
Fsp4HI	GCNGC	9 cut(s)	365, 516, 537, 652, 718, 771, 862, 943, 1221
FspBI	CTAG	4 cut(s)	5, 519, 672, 959
GlaI	GCGC	1 cut(s)	650
GluI	GCNGC	9 cut(s)	365, 516, 537, 652, 718, 771, 862, 943, 1221
GsaI	CCCAGC	2 cut(s)	290, 724
GsuI	CTGGAG	1 cut(s)	338
HaeIII	GGCC	6 cut(s)	391, 717, 738, 848, 1192, 1250
HapII	CCGG	7 cut(s)	126, 300, 382, 394, 400, 441, 663
HgaI	GACGC	1 cut(s)	1044
HhaI	GCGC	1 cut(s)	651
Hin1I	GRCGYC	2 cut(s)	36, 1055
Hin1II	CATG	4 cut(s)	1043, 1087, 1204, 1297
Hin6I	GCGC	1 cut(s)	649
HinP1I	GCGC	1 cut(s)	649
HincII	GTYRAC	1 cut(s)	1144
HindII	GTYRAC	1 cut(s)	1144
HindIII	AAGCTT	2 cut(s)	59, 94
HinfI	GANTC	6 cut(s)	47, 452, 696, 812, 1233, 1300
HpaI	GTTAAC	1 cut(s)	1144
HpaII	CCGG	7 cut(s)	126, 300, 382, 394, 400, 441, 663
HphI	GGTGA	4 cut(s)	109, 793, 1311, 1321
Hpy166II	GTNNAC	2 cut(s)	484, 1144
Hpy188I	TCNGA	8 cut(s)	91, 171, 207, 844, 882, 913, 1060, 1385
Hpy188III	TCNNGA	6 cut(s)	44, 306, 672, 875, 1230, 1378
Hpy8I	GTNNAC	2 cut(s)	484, 1144
HpyAV	CCTTC	4 cut(s)	672, 748, 913, 1248
HpyCH4III	ACNGT	2 cut(s)	203, 1323
HpyCH4IV	ACGT	3 cut(s)	36, 180, 425
HpyCH4V	TGCA	7 cut(s)	356, 640, 706, 783, 1066, 1220, 1352
HpyF10VI	GCNNNNNNNGC	6 cut(s)	93, 226, 512, 521, 646, 969
HpyF3I	CTNAG	4 cut(s)	273, 558, 843, 1059
HpySE526I	ACGT	3 cut(s)	36, 180, 425
Hsp92I	GRCGYC	2 cut(s)	36, 1055
Hsp92II	CATG	4 cut(s)	1043, 1087, 1204, 1297
HspAI	GCGC	1 cut(s)	649
KroI	GCCGGC	1 cut(s)	125
KroNI	GCCGGC	1 cut(s)	127
Ksp22I	TGATCA	1 cut(s)	190
KspAI	GTTAAC	1 cut(s)	1144
Kzo9I	GATC	7 cut(s)	7, 78, 190, 529, 540, 877, 1380
LguI	GCTCTTC	3 cut(s)	227, 253, 371
LmnI	GCTCC	7 cut(s)	92, 172, 313, 319, 340, 402, 901
Lsp1109I	GCAGC	7 cut(s)	376, 502, 523, 757, 873, 954, 1232
LweI	GCATC	2 cut(s)	1075, 1232
MaeI	CTAG	4 cut(s)	5, 519, 672, 959
MaeII	ACGT	3 cut(s)	36, 180, 425
MaeIII	GTNAC	6 cut(s)	421, 697, 799, 965, 1270, 1375
MalI	GATC	7 cut(s)	9, 80, 192, 531, 542, 879, 1382
MbiI	CCGCTC	1 cut(s)	229
MboI	GATC	7 cut(s)	7, 78, 190, 529, 540, 877, 1380
MflI	RGATCY	1 cut(s)	7
MhlI	GDGCHC	2 cut(s)	393, 407
MluCI	AATT	7 cut(s)	545, 616, 632, 686, 891, 934, 1087
MlyI	GAGTC	1 cut(s)	705
MmeI	TCCRAC	2 cut(s)	135, 185
MreI	CGCCGGCG	1 cut(s)	125
MroNI	GCCGGC	1 cut(s)	125
MseI	TTAA	4 cut(s)	570, 603, 836, 1143
MslI	CAYNNNNRTG	2 cut(s)	711, 1109
MspA1I	CMGCKG	3 cut(s)	188, 720, 1268
MspI	CCGG	7 cut(s)	126, 300, 382, 394, 400, 441, 663
MspR9I	CCNGG	5 cut(s)	394, 400, 442, 664, 767
Mva1269I	GAATGC	1 cut(s)	972
MvaI	CCWGG	1 cut(s)	767
MwoI	GCNNNNNNNGC	6 cut(s)	93, 226, 512, 521, 646, 969
NaeI	GCCGGC	1 cut(s)	127
NciI	CCSGG	4 cut(s)	394, 400, 442, 664
NdeI	CATATG	3 cut(s)	760, 823, 1110
NdeII	GATC	7 cut(s)	7, 78, 190, 529, 540, 877, 1380
NgoMIV	GCCGGC	1 cut(s)	125
NlaIII	CATG	4 cut(s)	1043, 1087, 1204, 1297
NlaIV	GGNNCC	5 cut(s)	304, 321, 391, 392, 398
NmuCI	GTSAC	5 cut(s)	697, 799, 965, 1270, 1375
OliI	CACNNNNGTG	1 cut(s)	711
PciSI	GCTCTTC	3 cut(s)	227, 253, 371
PcsI	WCGNNNNNNNCGW	6 cut(s)	30, 42, 87, 129, 237, 260
PctI	GAATGC	1 cut(s)	972
PdiI	GCCGGC	1 cut(s)	127
PfeI	GAWTC	5 cut(s)	47, 452, 812, 1233, 1300
Pfl23II	CGTACG	1 cut(s)	241
PflMI	CCANNNNNTGG	1 cut(s)	745
PfoI	TCCNGGA	1 cut(s)	398
PkrI	GCNGC	9 cut(s)	366, 517, 538, 653, 719, 772, 863, 944, 1222
Ple19I	CGATCG	2 cut(s)	81, 1383
PleI	GAGTC	1 cut(s)	704
PpsI	GAGTC	1 cut(s)	704
PshAI	GACNNNNGTC	1 cut(s)	295
Psp124BI	GAGCTC	1 cut(s)	407
Psp6I	CCWGG	1 cut(s)	765
PspFI	CCCAGC	2 cut(s)	286, 720
PspGI	CCWGG	1 cut(s)	765
PspLI	CGTACG	1 cut(s)	241
PspN4I	GGNNCC	5 cut(s)	304, 321, 391, 392, 398
PspOMI	GGGCCC	1 cut(s)	389
PspPI	GGNCC	6 cut(s)	210, 389, 390, 396, 736, 1190
PstI	CTGCAG	1 cut(s)	1222
PstNI	CAGNNNCTG	2 cut(s)	286, 1358
PsuI	RGATCY	1 cut(s)	7
PvuI	CGATCG	2 cut(s)	81, 1383
PvuII	CAGCTG	1 cut(s)	1268
RsaI	GTAC	3 cut(s)	243, 485, 661
RsaNI	GTAC	3 cut(s)	242, 484, 660
RseI	CAYNNNNRTG	2 cut(s)	711, 1109
SacI	GAGCTC	1 cut(s)	407
SapI	GCTCTTC	3 cut(s)	227, 253, 371
SaqAI	TTAA	4 cut(s)	570, 603, 836, 1143
SatI	GCNGC	9 cut(s)	365, 516, 537, 652, 718, 771, 862, 943, 1221
Sau3AI	GATC	7 cut(s)	7, 78, 190, 529, 540, 877, 1380
Sau96I	GGNCC	6 cut(s)	210, 389, 390, 396, 736, 1190
SchI	GAGTC	1 cut(s)	705
ScrFI	CCNGG	5 cut(s)	394, 400, 442, 664, 767
SduI	GDGCHC	2 cut(s)	393, 407
SfaNI	GCATC	2 cut(s)	1075, 1232
SfcI	CTRYAG	1 cut(s)	1218
SgrAI	CRCCGGYG	1 cut(s)	125
SinI	GGWCC	2 cut(s)	210, 396
SmiMI	CAYNNNNRTG	2 cut(s)	711, 1109
Sse9I	AATT	7 cut(s)	545, 616, 632, 686, 891, 934, 1087
SsiI	CCGC	9 cut(s)	147, 186, 227, 362, 471, 652, 718, 1188, 1306
SspMI	CTAG	4 cut(s)	5, 519, 672, 959
SstI	GAGCTC	1 cut(s)	407
StyD4I	CCNGG	5 cut(s)	392, 398, 440, 662, 765
TaaI	ACNGT	2 cut(s)	203, 1323
TaiI	ACGT	3 cut(s)	39, 183, 428
TaqI	TCGA	5 cut(s)	81, 433, 532, 683, 810
TasI	AATT	7 cut(s)	545, 616, 632, 686, 891, 934, 1087
TatI	WGTACW	1 cut(s)	483
TauI	GCSGC	2 cut(s)	654, 720
TfiI	GAWTC	5 cut(s)	47, 452, 812, 1233, 1300
Tru1I	TTAA	4 cut(s)	570, 603, 836, 1143
Tru9I	TTAA	4 cut(s)	570, 603, 836, 1143
TscAI	CASTG	4 cut(s)	287, 508, 583, 589
TseFI	GTSAC	5 cut(s)	697, 799, 965, 1270, 1375
TseI	GCWGC	7 cut(s)	364, 515, 536, 770, 861, 942, 1220
Tsp45I	GTSAC	5 cut(s)	697, 799, 965, 1270, 1375
TspDTI	ATGAA	4 cut(s)	1097, 1100, 1245, 1271
TspGWI	ACGGA	3 cut(s)	229, 539, 997
TspRI	CASTG	4 cut(s)	287, 508, 583, 589
Van91I	CCANNNNNTGG	1 cut(s)	745
VpaK11BI	GGWCC	2 cut(s)	210, 396
XapI	RAATTY	4 cut(s)	545, 632, 686, 1087
XbaI	TCTAGA	1 cut(s)	671
XcmI	CCANNNNNNNNNTGG	2 cut(s)	313, 1181
XspI	CTAG	4 cut(s)	5, 519, 672, 959
ZraI	GACGTC	1 cut(s)	37

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.