MD12G1150800.v1.1

Uracil phosphoribosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0011897
Physical Location & Seq
Forward (+)
23073971 .. 23077559
3589 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1150800.v1.1.491

Sequence Viewer

Length: 900 bp
ATGTCTTCTGCACCAATCTATCCAATCCTAATGGCGTGCCGTATTAATTTCTCTCTCCGGTGTCCCTCCTCCGAAACGCCGCGTTTCGGTTCCACTTGTCGCCTCCAAAACCCTAGCTCCTCTCTCCTACTTAATCTCGCTCCCGCCTCTTCTTCTTCTTCGAATCCCAAAAAGATCTCGTATTCCGCTACGCGGCGGCGCAGCTCCGTTGCGGTGAAGTCTCAAATGGCCACGGAGGAGAAATTGGCTTCCGGGAACAGAATGCTTGTTTTCGTGCCACCGCACCCGTTGATCAAGCATTGGGTTTCGGTTTTGAGAAATGAGCAAACCCCTTGTCCGATTTTTCGAAATGCCATGGCTGAGTTGGGGAGGCTTCTTCTGTATGAAGCTTCAAGAGATTGGCTGCCTACGGTAACAGGAGAGATTCAGTCACCAATGGGTGTCGCATCAGTTGAATTTGTAGATCCAAGGGAGCCTATTTCGGTTGTTCCAATTTTGAGAGCTGGTCTGGCTCTTGTAGAACATGCATCGTCAATCTTGCCAGCCACAAAGACGTACCATCTTGGGTTTAGCAGAGATGAGGAGACACTTCTACCAACTGTATATCTGAACAAGTTACCTGACAATTTTCCGGAAGGCTCTCGAGTATTTTTGGTTGACCCTATGCTAGCAACAGGAGGCACAATAGTAGCGGCTTTGGATCTTTTAAAGGAATGCGGGCTTGACAACAAGCAGATCAAAGTGATATCTGCTGTAGCTGCGCCTCCAGCACTTCAAAAGCTGAGCGAGAAGTTTCCAGGGCTTCATGTATACACTGGAATTATTGATCCTACCGTAAATGAGAAAGGGTTTATAATCCCTGGACTGGGTGATGCTGGAGACCGCAGCTTCGGTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004845 GO:0004849 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006206 GO:0006213 GO:0006220 GO:0006221 GO:0006222 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0008655 GO:0009058 GO:0009112 GO:0009116 GO:0009117 GO:0009119 GO:0009123 GO:0009124 GO:0009129 GO:0009130 GO:0009156 GO:0009161 GO:0009163 GO:0009165 GO:0009173 GO:0009174 GO:0009218 GO:0009220 GO:0009259 GO:0009260 GO:0009267 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009987 GO:0009991 GO:0016036 GO:0016301 GO:0016310 GO:0016740 GO:0016757 GO:0016763 GO:0016772 GO:0018130 GO:0019205 GO:0019206 GO:0019438 GO:0019637 GO:0019693 GO:0031667 GO:0031668 GO:0031669 GO:0032502 GO:0033554 GO:0034404 GO:0034641 GO:0034654 GO:0042455 GO:0042594 GO:0043094 GO:0043097 GO:0043174 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046049 GO:0046131 GO:0046132 GO:0046134 GO:0046390 GO:0046483 GO:0050896 GO:0051716 GO:0055086 GO:0071496 GO:0071704 GO:0072527 GO:0072528 GO:0090407 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659
KEGG Pathways
Metabolic & Signaling
ko00240 ko01100 map00240 map01100
Pfam Domains
Protein Families
UPRTase

Protein Analysis

300

Amino Acids

32.4

Weight (kDa)

8.79

Isoelectric Point (pI)

53.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPRTase PF14681 92 - 299 3.8e-67 Uracil phosphoribosyltransferase
Pribosyltran PF00156 156 - 254 7.5e-13 Phosphoribosyl transferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011897)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G53900 AT3G53900
fragaria_vesca FvH4_6g12970 FvH4_6g12970
malus_domestica MD04G1136300.v1.1 MD12G1150800.v1.1
prunus_persica Prupe.6G262400_v2.0.a1
pyrus_communis pycom12g14470
rosa_chinensis RchiOBHm_Chr3g0465081
rosa_laevigata RLG00000024657
rosa_multiflora Rmu_sc0004362.1_g000003
rosa_roxburghii Rroxscaffold_6G00415260
rosa_rugosa Rorug03G0075100 Rorug03G0075200
rosa_samantha Rh3BG138900 Rh3CG141000 Rh3CG149300 Rh3DG140100
rosa_wichuraiana Rw3G011360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 854
AccI GTMKAC 1 cut(s) 810
AccII CGCG 2 cut(s) 82, 193
AccIII TCCGGA 1 cut(s) 631
AclWI GGATC 3 cut(s) 458, 708, 821
AcoI YGGCCR 1 cut(s) 228
AcsI RAATTY 1 cut(s) 455
AfaI GTAC 2 cut(s) 557, 895
AfiI CCNNNNNNNGG 4 cut(s) 86, 192, 865, 866
AgsI TTSAA 3 cut(s) 393, 455, 776
AjnI CCWGG 2 cut(s) 796, 859
AluBI AGCT 7 cut(s) 117, 204, 389, 503, 758, 781, 888
AluI AGCT 7 cut(s) 117, 204, 389, 503, 758, 781, 888
Alw26I GTCTC 3 cut(s) 225, 578, 873
AlwI GGATC 3 cut(s) 458, 708, 821
Ama87I CYCGRG 1 cut(s) 642
Aor13HI TCCGGA 1 cut(s) 631
AoxI GGCC 1 cut(s) 228
ApeKI GCWGC 4 cut(s) 201, 403, 758, 885
ApoI RAATTY 1 cut(s) 455
ArsI GACNNNNNNTTYG 3 cut(s) 319, 351, 872
AseI ATTAAT 1 cut(s) 45
AspLEI GCGC 2 cut(s) 201, 763
AsuC2I CCSGG 1 cut(s) 253
AsuHPI GGTGA 3 cut(s) 226, 423, 881
AsuII TTCGAA 2 cut(s) 161, 346
AsuNHI GCTAGC 1 cut(s) 667
AvaI CYCGRG 1 cut(s) 642
BalI TGGCCA 1 cut(s) 230
BbvI GCAGC 3 cut(s) 213, 390, 745
BccI CCATC 1 cut(s) 567
BceAI ACGGC 1 cut(s) 24
BciT130I CCWGG 2 cut(s) 798, 861
BclI TGATCA 1 cut(s) 291
BcnI CCSGG 1 cut(s) 253
BcoDI GTCTC 3 cut(s) 225, 578, 873
BfaI CTAG 2 cut(s) 114, 668
BfmI CTRYAG 1 cut(s) 753
BglII AGATCT 1 cut(s) 174
BisI GCNGC 8 cut(s) 80, 194, 197, 202, 404, 693, 759, 886
BlpI GCTNAGC 1 cut(s) 782
BlsI GCNGC 8 cut(s) 81, 195, 198, 203, 405, 694, 760, 887
Bme1390I CCNGG 3 cut(s) 253, 798, 861
BmeT110I CYCGRG 1 cut(s) 642
BmiI GGNNCC 2 cut(s) 91, 474
BmrFI CCNGG 3 cut(s) 253, 798, 861
BmrI ACTGGG 1 cut(s) 875
BmsI GCATC 3 cut(s) 455, 536, 862
BmtI GCTAGC 1 cut(s) 671
BmuI ACTGGG 1 cut(s) 875
BpmI CTGGAG 2 cut(s) 750, 897
Bpu1102I GCTNAGC 1 cut(s) 782
Bpu14I TTCGAA 2 cut(s) 161, 346
BpuMI CCSGG 1 cut(s) 253
BsaI GGTCTC 1 cut(s) 873
BsaJI CCNNGG 5 cut(s) 231, 354, 467, 797, 859
BsaWI WCCGGW 2 cut(s) 57, 631
BsaXI ACNNNNNCTCC 2 cut(s) 101, 131
Bsc4I CCNNNNNNNGG 4 cut(s) 86, 192, 865, 866
Bse1I ACTGG 2 cut(s) 820, 870
BseAI TCCGGA 1 cut(s) 631
BseBI CCWGG 2 cut(s) 798, 861
BseDI CCNNGG 5 cut(s) 231, 354, 467, 797, 859
BseLI CCNNNNNNNGG 4 cut(s) 86, 192, 865, 866
BseMII CTCAG 2 cut(s) 351, 773
BseNI ACTGG 2 cut(s) 820, 870
BseRI GAGGAG 4 cut(s) 58, 109, 251, 596
BseXI GCAGC 3 cut(s) 213, 390, 745
Bsh1236I CGCG 2 cut(s) 82, 193
BshFI GGCC 1 cut(s) 230
BsiHKCI CYCGRG 1 cut(s) 642
BsiSI CCGG 3 cut(s) 58, 252, 632
BslFI GGGAC 1 cut(s) 48
BslI CCNNNNNNNGG 4 cut(s) 86, 192, 865, 866
BsmAI GTCTC 3 cut(s) 225, 578, 873
BsmFI GGGAC 1 cut(s) 48
BsmI GAATGC 2 cut(s) 267, 719
BsnI GGCC 1 cut(s) 230
Bso31I GGTCTC 1 cut(s) 873
BsoBI CYCGRG 1 cut(s) 642
Bsp119I TTCGAA 2 cut(s) 161, 346
Bsp13I TCCGGA 1 cut(s) 631
Bsp143I GATC 6 cut(s) 174, 291, 463, 700, 735, 826
Bsp1720I GCTNAGC 1 cut(s) 782
Bsp19I CCATGG 1 cut(s) 354
BspANI GGCC 1 cut(s) 230
BspCNI CTCAG 2 cut(s) 352, 774
BspEI TCCGGA 1 cut(s) 631
BspFNI CGCG 2 cut(s) 82, 193
BspLI GGNNCC 2 cut(s) 91, 474
BspOI GCTAGC 1 cut(s) 671
BspPI GGATC 3 cut(s) 458, 708, 821
BspT104I TTCGAA 2 cut(s) 161, 346
BspTNI GGTCTC 1 cut(s) 873
BsrI ACTGG 2 cut(s) 820, 870
BssECI CCNNGG 5 cut(s) 231, 354, 467, 797, 859
BssMI GATC 6 cut(s) 174, 291, 463, 700, 735, 826
BssNAI GTATAC 1 cut(s) 811
BssT1I CCWWGG 2 cut(s) 354, 467
Bst1107I GTATAC 1 cut(s) 811
Bst2UI CCWGG 2 cut(s) 798, 861
Bst4CI ACNGT 3 cut(s) 412, 601, 835
Bst6I CTCTTC 1 cut(s) 154
BstBI TTCGAA 2 cut(s) 161, 346
BstC8I GCNNGC 4 cut(s) 37, 543, 669, 719
BstDEI CTNAG 2 cut(s) 360, 782
BstDSI CCRYGG 2 cut(s) 231, 354
BstFNI CGCG 2 cut(s) 82, 193
BstHHI GCGC 2 cut(s) 201, 763
BstKTI GATC 6 cut(s) 177, 294, 466, 703, 738, 829
BstMAI GTCTC 3 cut(s) 225, 578, 873
BstMBI GATC 6 cut(s) 174, 291, 463, 700, 735, 826
BstMWI GCNNNNNNNGC 3 cut(s) 509, 758, 767
BstNI CCWGG 2 cut(s) 798, 861
BstNSI RCATGY 1 cut(s) 527
BstSCI CCNGG 3 cut(s) 251, 796, 859
BstSFI CTRYAG 1 cut(s) 753
BstUI CGCG 2 cut(s) 82, 193
BstV1I GCAGC 3 cut(s) 213, 390, 745
BstX2I RGATCY 3 cut(s) 174, 463, 700
BstYI RGATCY 3 cut(s) 174, 463, 700
BstZ17I GTATAC 1 cut(s) 811
BsuRI GGCC 1 cut(s) 230
BtgI CCRYGG 2 cut(s) 231, 354
BtsIMutI CAGTG 1 cut(s) 813
Cac8I GCNNGC 4 cut(s) 37, 543, 669, 719
CfoI GCGC 2 cut(s) 201, 763
Csp6I GTAC 2 cut(s) 556, 894
CviAII CATG 3 cut(s) 355, 524, 806
CviQI GTAC 2 cut(s) 556, 894
DdeI CTNAG 2 cut(s) 360, 782
DpnI GATC 6 cut(s) 176, 293, 465, 702, 737, 828
DpnII GATC 6 cut(s) 174, 291, 463, 700, 735, 826
DraI TTTAAA 1 cut(s) 708
EaeI YGGCCR 1 cut(s) 228
Eam1104I CTCTTC 1 cut(s) 154
EarI CTCTTC 1 cut(s) 154
Eco130I CCWWGG 2 cut(s) 354, 467
Eco31I GGTCTC 1 cut(s) 873
Eco32I GATATC 1 cut(s) 747
Eco88I CYCGRG 1 cut(s) 642
EcoRII CCWGG 2 cut(s) 796, 859
EcoRV GATATC 1 cut(s) 747
EcoT14I CCWWGG 2 cut(s) 354, 467
EcoT22I ATGCAT 1 cut(s) 529
ErhI CCWWGG 2 cut(s) 354, 467
FaeI CATG 3 cut(s) 358, 527, 809
FaiI YATR 9 cut(s) 356, 384, 525, 604, 665, 807, 811, 854, 898
FaqI GGGAC 1 cut(s) 48
FatI CATG 3 cut(s) 354, 523, 805
FauI CCCGC 2 cut(s) 151, 710
FbaI TGATCA 1 cut(s) 291
FblI GTMKAC 1 cut(s) 810
Fnu4HI GCNGC 8 cut(s) 80, 194, 197, 202, 404, 693, 759, 886
Fsp4HI GCNGC 8 cut(s) 80, 194, 197, 202, 404, 693, 759, 886
FspBI CTAG 2 cut(s) 114, 668
GlaI GCGC 2 cut(s) 200, 762
GluI GCNGC 8 cut(s) 80, 194, 197, 202, 404, 693, 759, 886
GsuI CTGGAG 2 cut(s) 750, 897
HaeIII GGCC 1 cut(s) 230
HapII CCGG 3 cut(s) 58, 252, 632
HhaI GCGC 2 cut(s) 201, 763
Hin1II CATG 3 cut(s) 358, 527, 809
Hin6I GCGC 2 cut(s) 199, 761
HinP1I GCGC 2 cut(s) 199, 761
HincII GTYRAC 1 cut(s) 658
HindII GTYRAC 1 cut(s) 658
HindIII AAGCTT 1 cut(s) 387
HinfI GANTC 2 cut(s) 163, 424
HpaII CCGG 3 cut(s) 58, 252, 632
HphI GGTGA 3 cut(s) 226, 423, 881
Hpy166II GTNNAC 2 cut(s) 658, 811
Hpy188I TCNGA 3 cut(s) 73, 339, 609
Hpy188III TCNNGA 3 cut(s) 393, 632, 642
Hpy8I GTNNAC 2 cut(s) 658, 811
HpyAV CCTTC 1 cut(s) 629
HpyCH4III ACNGT 3 cut(s) 412, 601, 835
HpyCH4IV ACGT 1 cut(s) 554
HpyCH4V TGCA 2 cut(s) 11, 527
HpyF10VI GCNNNNNNNGC 3 cut(s) 509, 758, 767
HpyF3I CTNAG 2 cut(s) 360, 782
HpySE526I ACGT 1 cut(s) 554
Hsp92II CATG 3 cut(s) 358, 527, 809
HspAI GCGC 2 cut(s) 199, 761
Kpn2I TCCGGA 1 cut(s) 631
Ksp22I TGATCA 1 cut(s) 291
Kzo9I GATC 6 cut(s) 174, 291, 463, 700, 735, 826
LmnI GCTCC 4 cut(s) 122, 145, 209, 472
Lsp1109I GCAGC 3 cut(s) 213, 390, 745
LweI GCATC 3 cut(s) 455, 536, 862
MaeI CTAG 2 cut(s) 114, 668
MaeII ACGT 1 cut(s) 554
MaeIII GTNAC 3 cut(s) 412, 429, 615
MalI GATC 6 cut(s) 176, 293, 465, 702, 737, 828
MboI GATC 6 cut(s) 174, 291, 463, 700, 735, 826
MboII GAAGA 5 cut(s) 141, 144, 147, 150, 368
MflI RGATCY 3 cut(s) 174, 463, 700
MlsI TGGCCA 1 cut(s) 230
MluCI AATT 6 cut(s) 46, 242, 455, 492, 625, 819
MluNI TGGCCA 1 cut(s) 230
Mox20I TGGCCA 1 cut(s) 230
Mph1103I ATGCAT 1 cut(s) 529
MroI TCCGGA 1 cut(s) 631
MscI TGGCCA 1 cut(s) 230
MseI TTAA 3 cut(s) 45, 132, 707
Msp20I TGGCCA 1 cut(s) 230
MspI CCGG 3 cut(s) 58, 252, 632
MspR9I CCNGG 3 cut(s) 253, 798, 861
Mva1269I GAATGC 2 cut(s) 267, 719
MvaI CCWGG 2 cut(s) 798, 861
MvnI CGCG 2 cut(s) 82, 193
MwoI GCNNNNNNNGC 3 cut(s) 509, 758, 767
NciI CCSGG 1 cut(s) 253
NcoI CCATGG 1 cut(s) 354
NdeII GATC 6 cut(s) 174, 291, 463, 700, 735, 826
NheI GCTAGC 1 cut(s) 667
NlaIII CATG 3 cut(s) 358, 527, 809
NlaIV GGNNCC 2 cut(s) 91, 474
NmuCI GTSAC 1 cut(s) 429
NsiI ATGCAT 1 cut(s) 529
NspI RCATGY 1 cut(s) 527
NspV TTCGAA 2 cut(s) 161, 346
PaeR7I CTCGAG 1 cut(s) 642
PctI GAATGC 2 cut(s) 267, 719
PfeI GAWTC 2 cut(s) 163, 424
PfoI TCCNGGA 1 cut(s) 251
PkrI GCNGC 8 cut(s) 81, 195, 198, 203, 405, 694, 760, 887
PshBI ATTAAT 1 cut(s) 45
PsiI TTATAA 1 cut(s) 854
Psp6I CCWGG 2 cut(s) 796, 859
PspGI CCWGG 2 cut(s) 796, 859
PspN4I GGNNCC 2 cut(s) 91, 474
PsuI RGATCY 3 cut(s) 174, 463, 700
RsaI GTAC 2 cut(s) 557, 895
RsaNI GTAC 2 cut(s) 556, 894
SaqAI TTAA 3 cut(s) 45, 132, 707
SatI GCNGC 8 cut(s) 80, 194, 197, 202, 404, 693, 759, 886
Sau3AI GATC 6 cut(s) 174, 291, 463, 700, 735, 826
ScrFI CCNGG 3 cut(s) 253, 798, 861
SetI ASST 9 cut(s) 119, 206, 391, 505, 557, 622, 760, 783, 890
SfaNI GCATC 3 cut(s) 455, 536, 862
SfcI CTRYAG 1 cut(s) 753
Sfr274I CTCGAG 1 cut(s) 642
SfuI TTCGAA 2 cut(s) 161, 346
SlaI CTCGAG 1 cut(s) 642
SmlI CTYRAG 1 cut(s) 642
SmoI CTYRAG 1 cut(s) 642
Sse9I AATT 6 cut(s) 46, 242, 455, 492, 625, 819
SspMI CTAG 2 cut(s) 114, 668
StyD4I CCNGG 3 cut(s) 251, 796, 859
StyI CCWWGG 2 cut(s) 354, 467
TaaI ACNGT 3 cut(s) 412, 601, 835
TaiI ACGT 1 cut(s) 557
TaqI TCGA 3 cut(s) 161, 346, 643
TasI AATT 6 cut(s) 46, 242, 455, 492, 625, 819
TauI GCSGC 4 cut(s) 82, 196, 199, 695
TfiI GAWTC 2 cut(s) 163, 424
Tru1I TTAA 3 cut(s) 45, 132, 707
Tru9I TTAA 3 cut(s) 45, 132, 707
TscAI CASTG 1 cut(s) 820
TseFI GTSAC 1 cut(s) 429
TseI GCWGC 4 cut(s) 201, 403, 758, 885
Tsp45I GTSAC 1 cut(s) 429
TspDTI ATGAA 2 cut(s) 399, 794
TspGWI ACGGA 2 cut(s) 196, 248
TspRI CASTG 1 cut(s) 820
VspI ATTAAT 1 cut(s) 45
XapI RAATTY 1 cut(s) 455
XceI RCATGY 1 cut(s) 527
XcmI CCANNNNNNNNNTGG 1 cut(s) 361
XhoI CTCGAG 1 cut(s) 642
XmiI GTMKAC 1 cut(s) 810
XspI CTAG 2 cut(s) 114, 668
Zsp2I ATGCAT 1 cut(s) 529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.