MD12G1153000.v1.1

receptor-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0016941
Physical Location & Seq
Reverse (-)
23298176 .. 23300744
2569 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1153000.v1.1.491

Sequence Viewer

Length: 1350 bp
ATGGCTTCTTCGAGTTCAAGTTCTGCAGTTCGATTGTTGGAGTATTGTGGATCCTTTCCACTTAATGAACTTCAACAACTAAAAAATCTTTGGAGTCTTGATCTGTCATACAACAGCTTGTTGATTACTTATGCCGATACTAATTCCTCTCATTCTTCCTTTCCTCAACTTACGACATTGAAATTGTCTTTTGGAAAGTTGAGAACATTTCCAGATTTCTTGAGAAGTCAATCAAAATTAAGCACTTTGGACCTTTCCTCAAACCAGATTCATGGTGAAATACCCAACTGGATTTGGAAGTTGAGTAGTCTTAGTCAACTTAATCTTTCTTGCAACTCTCTGGTAACTCTAGAAGGTCCTTTCCTCAATATCACTTCTAATTTGTCTGCGTTTGACCTTCATTCCAACCAGCTTAAGGGACCAATTCCAATGTTTCCACGATTGGCCACTTATCTGGATTACTCAAGAAATAATTTCAGCTCTAGCATTCCGGCTGACATTGGTGATTTCCTTATGTACACTGTGTTCTTCTCTCTTGAAAGCAATCACTTCCACGGGATCATTCCAGCATCAGTATGTCGTGCAACATATCTTCAAGTTCTTGATTTGTCCAATAATTTGTTAAGTGGCATGATTCCCCAGTGCTTCACTACAAGGAACAGGTATCTTGCAGTACTTAGAGGTGGTTTGGGAGTGAGCACAGCTGCTTATTCTCATAGCAGCTTTTTTTCAAAGCACATCAATACCAAACCAGCCCTTAATTTAAGGGGAAACAAACTTTCTGGCACTCTTCCTGATAATTTTCCTGAGCGATGTAGTCTACGAAGTCTAGACCTCAATGGCAATGTGATCGGTGGTCAGTTCCCAAAGTCTCTAGTCAATTGCAGAATGTTAGAGGTGTTAAACTTGGGAAACAATCAGATAACAGATGTCTTTCCGTGCTTATTGAAAAGCATATCCAGCTTGCGTGTGCTTATCTTGCAACAACAACAACAACAACAACAACAAAGCCTTTTCCCACTAAGTGGGGTCGGCTATATGAATCCTAGAACGCCATTGCGCTTGGTTTTGTGTCACGTCCTCCGTAGATGTGGCATCTTCTCTGGAAGTACTCTTCCTCCATCCAGGGGTGGTATCTGTAACTGGCCAAAGCTTATCTTGCGTTCCAACAAATTTTATGGTGGTGTTGATTGCAACAGCTTCAAGCCCAATGCTTCCTGGCCAAAGCTTCCAATTTTTGACGTAGCTGACAACAATTTCACTGGCAAATTACCATCATTTGGTTTGAAAACCATGAAAGCACTGATGGATGAAGAAGATGATGCCGTGTCAAAGATGACTCACCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009888 GO:0010073 GO:0032501 GO:0032502 GO:0048507 GO:0048856
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8

Protein Analysis

450

Amino Acids

49.89

Weight (kDa)

9.21

Isoelectric Point (pI)

43.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 55 - 114 2.1e-07 Leucine rich repeat
LRR_8 PF13855 273 - 328 1.7e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 1025, 1280
AccI GTMKAC 1 cut(s) 820
AclWI GGATC 3 cut(s) 45, 58, 566
AcoI YGGCCR 3 cut(s) 444, 1145, 1220
AcsI RAATTY 1 cut(s) 1172
AdeI CACNNNGTG 1 cut(s) 1025
AfaI GTAC 3 cut(s) 518, 675, 1111
AfiI CCNNNNNNNGG 4 cut(s) 415, 1025, 1127, 1280
AflII CTTAAG 1 cut(s) 413
AgsI TTSAA 9 cut(s) 18, 74, 181, 539, 596, 732, 949, 1204, 1288
AjiI CACGTC 1 cut(s) 1078
AjnI CCWGG 2 cut(s) 1124, 1217
Alw21I GWGCWC 1 cut(s) 701
Alw26I GTCTC 1 cut(s) 876
AlwI GGATC 3 cut(s) 45, 58, 566
AoxI GGCC 3 cut(s) 444, 1145, 1220
ApeKI GCWGC 2 cut(s) 704, 720
ApoI RAATTY 1 cut(s) 1172
AspLEI GCGC 1 cut(s) 1062
AspS9I GGNCC 3 cut(s) 250, 356, 419
AsuHPI GGTGA 3 cut(s) 287, 515, 1334
AvaII GGWCC 3 cut(s) 250, 356, 419
BalI TGGCCA 3 cut(s) 446, 1147, 1222
BamHI GGATCC 1 cut(s) 50
Bbv12I GWGCWC 1 cut(s) 701
BbvI GCAGC 2 cut(s) 691, 732
BccI CCATC 3 cut(s) 1129, 1282, 1300
BceAI ACGGC 1 cut(s) 1310
BcgI CGANNNNNNTGC 2 cut(s) 832, 866
BciT130I CCWGG 2 cut(s) 1126, 1219
BcoDI GTCTC 1 cut(s) 876
BfaI CTAG 5 cut(s) 350, 483, 830, 875, 1047
BfmI CTRYAG 1 cut(s) 24
BfrI CTTAAG 1 cut(s) 413
BisI GCNGC 2 cut(s) 705, 721
BlsI GCNGC 2 cut(s) 706, 722
BmcAI AGTACT 2 cut(s) 675, 1111
Bme1390I CCNGG 2 cut(s) 1126, 1219
Bme18I GGWCC 3 cut(s) 250, 356, 419
BmgBI CACGTC 1 cut(s) 1078
BmgT120I GGNCC 3 cut(s) 250, 356, 419
BmiI GGNNCC 2 cut(s) 52, 420
BmrFI CCNGG 2 cut(s) 1126, 1219
BmrI ACTGGG 1 cut(s) 634
BmsI GCATC 3 cut(s) 578, 1104, 1312
BmuI ACTGGG 1 cut(s) 634
Bpu10I CCTNAGC 1 cut(s) 807
BpuEI CTTGAG 2 cut(s) 241, 448
BsaJI CCNNGG 2 cut(s) 553, 1125
BsaXI ACNNNNNCTCC 2 cut(s) 1102, 1132
Bsc4I CCNNNNNNNGG 4 cut(s) 415, 1025, 1127, 1280
Bse1I ACTGG 4 cut(s) 293, 640, 1148, 1267
Bse3DI GCAATG 2 cut(s) 850, 1055
BseBI CCWGG 2 cut(s) 1126, 1219
BseDI CCNNGG 2 cut(s) 553, 1125
BseGI GGATG 2 cut(s) 1121, 1315
BseLI CCNNNNNNNGG 4 cut(s) 415, 1025, 1127, 1280
BseMI GCAATG 2 cut(s) 850, 1055
BseMII CTCAG 1 cut(s) 798
BseNI ACTGG 4 cut(s) 293, 640, 1148, 1267
BseXI GCAGC 2 cut(s) 691, 732
BshFI GGCC 3 cut(s) 446, 1147, 1222
BsiHKAI GWGCWC 1 cut(s) 701
BsiSI CCGG 1 cut(s) 491
BslFI GGGAC 1 cut(s) 432
BslI CCNNNNNNNGG 4 cut(s) 415, 1025, 1127, 1280
BsmAI GTCTC 1 cut(s) 876
BsmFI GGGAC 1 cut(s) 432
BsmI GAATGC 1 cut(s) 486
BsnI GGCC 3 cut(s) 446, 1147, 1222
Bsp1286I GDGCHC 1 cut(s) 701
Bsp1407I TGTACA 1 cut(s) 516
Bsp143I GATC 4 cut(s) 50, 100, 558, 849
BspANI GGCC 3 cut(s) 446, 1147, 1222
BspCNI CTCAG 1 cut(s) 799
BspLI GGNNCC 2 cut(s) 52, 420
BspMAI CTGCAG 1 cut(s) 28
BspPI GGATC 3 cut(s) 45, 58, 566
BspTI CTTAAG 1 cut(s) 413
BsrDI GCAATG 2 cut(s) 850, 1055
BsrGI TGTACA 1 cut(s) 516
BsrI ACTGG 4 cut(s) 293, 640, 1148, 1267
BssECI CCNNGG 2 cut(s) 553, 1125
BssMI GATC 4 cut(s) 50, 100, 558, 849
Bst2UI CCWGG 2 cut(s) 1126, 1219
Bst4CI ACNGT 1 cut(s) 523
Bst6I CTCTTC 2 cut(s) 795, 1119
BstAFI CTTAAG 1 cut(s) 413
BstAUI TGTACA 1 cut(s) 516
BstC8I GCNNGC 1 cut(s) 965
BstDEI CTNAG 4 cut(s) 311, 677, 807, 1022
BstDSI CCRYGG 1 cut(s) 553
BstF5I GGATG 2 cut(s) 1121, 1315
BstHHI GCGC 1 cut(s) 1062
BstKTI GATC 4 cut(s) 53, 103, 561, 852
BstMAI GTCTC 1 cut(s) 876
BstMBI GATC 4 cut(s) 50, 100, 558, 849
BstMWI GCNNNNNNNGC 3 cut(s) 960, 979, 1159
BstNI CCWGG 2 cut(s) 1126, 1219
BstSCI CCNGG 2 cut(s) 1124, 1217
BstSFI CTRYAG 1 cut(s) 24
BstV1I GCAGC 2 cut(s) 691, 732
BstX2I RGATCY 1 cut(s) 50
BstXI CCANNNNNNTGG 2 cut(s) 272, 454
BstYI RGATCY 1 cut(s) 50
BsuRI GGCC 3 cut(s) 446, 1147, 1222
BtgI CCRYGG 1 cut(s) 553
BtgZI GCGATG 1 cut(s) 826
BtrI CACGTC 1 cut(s) 1078
BtsCI GGATG 2 cut(s) 1121, 1315
BtsIMutI CAGTG 4 cut(s) 519, 647, 1260, 1301
Cac8I GCNNGC 1 cut(s) 965
CfoI GCGC 1 cut(s) 1062
Cfr13I GGNCC 3 cut(s) 250, 356, 419
Csp6I GTAC 3 cut(s) 517, 674, 1110
CviAII CATG 3 cut(s) 272, 631, 1294
CviQI GTAC 3 cut(s) 517, 674, 1110
DdeI CTNAG 4 cut(s) 311, 677, 807, 1022
DpnI GATC 4 cut(s) 52, 102, 560, 851
DpnII GATC 4 cut(s) 50, 100, 558, 849
DraIII CACNNNGTG 1 cut(s) 1025
EaeI YGGCCR 3 cut(s) 444, 1145, 1220
Eam1104I CTCTTC 2 cut(s) 795, 1119
EarI CTCTTC 2 cut(s) 795, 1119
Eco47I GGWCC 3 cut(s) 250, 356, 419
EcoO109I RGGNCCY 1 cut(s) 356
EcoRII CCWGG 2 cut(s) 1124, 1217
FaeI CATG 3 cut(s) 275, 634, 1297
FalI AAGNNNNNCTT 2 cut(s) 1142, 1174
FaqI GGGAC 1 cut(s) 432
FatI CATG 3 cut(s) 271, 630, 1293
FblI GTMKAC 1 cut(s) 820
Fnu4HI GCNGC 2 cut(s) 705, 721
FokI GGATG 2 cut(s) 1108, 1322
Fsp4HI GCNGC 2 cut(s) 705, 721
FspBI CTAG 5 cut(s) 350, 483, 830, 875, 1047
GlaI GCGC 1 cut(s) 1061
GluI GCNGC 2 cut(s) 705, 721
HaeIII GGCC 3 cut(s) 446, 1147, 1222
HapII CCGG 1 cut(s) 491
HhaI GCGC 1 cut(s) 1062
Hin1II CATG 3 cut(s) 275, 634, 1297
Hin6I GCGC 1 cut(s) 1060
HinP1I GCGC 1 cut(s) 1060
HincII GTYRAC 1 cut(s) 317
HindII GTYRAC 1 cut(s) 317
HindIII AAGCTT 2 cut(s) 1151, 1226
HinfI GANTC 5 cut(s) 94, 268, 634, 1042, 1339
HpaII CCGG 1 cut(s) 491
HphI GGTGA 3 cut(s) 287, 515, 1334
Hpy166II GTNNAC 3 cut(s) 317, 519, 821
Hpy188I TCNGA 1 cut(s) 921
Hpy8I GTNNAC 3 cut(s) 317, 519, 821
HpyAV CCTTC 2 cut(s) 347, 407
HpyCH4III ACNGT 1 cut(s) 523
HpyCH4IV ACGT 2 cut(s) 1077, 1242
HpyCH4V TGCA 7 cut(s) 26, 333, 584, 671, 885, 982, 1194
HpyF10VI GCNNNNNNNGC 3 cut(s) 960, 979, 1159
HpyF3I CTNAG 4 cut(s) 311, 677, 807, 1022
HpySE526I ACGT 2 cut(s) 1077, 1242
Hsp92II CATG 3 cut(s) 275, 634, 1297
HspAI GCGC 1 cut(s) 1060
Kzo9I GATC 4 cut(s) 50, 100, 558, 849
Lsp1109I GCAGC 2 cut(s) 691, 732
LweI GCATC 3 cut(s) 578, 1104, 1312
MaeI CTAG 5 cut(s) 350, 483, 830, 875, 1047
MaeII ACGT 2 cut(s) 1077, 1242
MaeIII GTNAC 3 cut(s) 343, 1073, 1139
MalI GATC 4 cut(s) 52, 102, 560, 851
MboI GATC 4 cut(s) 50, 100, 558, 849
MboII GAAGA 8 cut(s) 147, 520, 584, 782, 1090, 1106, 1325, 1328
MfeI CAATTG 1 cut(s) 880
MflI RGATCY 1 cut(s) 50
MhlI GDGCHC 1 cut(s) 701
MlsI TGGCCA 3 cut(s) 446, 1147, 1222
MluNI TGGCCA 3 cut(s) 446, 1147, 1222
MlyI GAGTC 2 cut(s) 103, 1333
MmeI TCCRAC 3 cut(s) 18, 429, 1191
MnlI CCTC 9 cut(s) 157, 174, 268, 374, 674, 845, 889, 1091, 1128
Mox20I TGGCCA 3 cut(s) 446, 1147, 1222
MscI TGGCCA 3 cut(s) 446, 1147, 1222
MseI TTAA 8 cut(s) 63, 239, 321, 414, 623, 759, 764, 902
MslI CAYNNNNRTG 1 cut(s) 574
Msp20I TGGCCA 3 cut(s) 446, 1147, 1222
MspA1I CMGCKG 1 cut(s) 704
MspCI CTTAAG 1 cut(s) 413
MspI CCGG 1 cut(s) 491
MspR9I CCNGG 2 cut(s) 1126, 1219
MunI CAATTG 1 cut(s) 880
Mva1269I GAATGC 1 cut(s) 486
MvaI CCWGG 2 cut(s) 1126, 1219
MwoI GCNNNNNNNGC 3 cut(s) 960, 979, 1159
NdeII GATC 4 cut(s) 50, 100, 558, 849
NlaIII CATG 3 cut(s) 275, 634, 1297
NlaIV GGNNCC 2 cut(s) 52, 420
NmuCI GTSAC 1 cut(s) 1073
PctI GAATGC 1 cut(s) 486
PfeI GAWTC 3 cut(s) 268, 634, 1042
PflMI CCANNNNNTGG 2 cut(s) 1025, 1280
PkrI GCNGC 2 cut(s) 706, 722
PleI GAGTC 2 cut(s) 102, 1333
PpsI GAGTC 2 cut(s) 102, 1333
PpuMI RGGWCCY 1 cut(s) 356
Psp5II RGGWCCY 1 cut(s) 356
Psp6I CCWGG 2 cut(s) 1124, 1217
PspGI CCWGG 2 cut(s) 1124, 1217
PspN4I GGNNCC 2 cut(s) 52, 420
PspPI GGNCC 3 cut(s) 250, 356, 419
PspPPI RGGWCCY 1 cut(s) 356
PsrI GAACNNNNNNTAC 2 cut(s) 509, 541
PstI CTGCAG 1 cut(s) 28
PsuI RGATCY 1 cut(s) 50
PvuII CAGCTG 1 cut(s) 704
RsaI GTAC 3 cut(s) 518, 675, 1111
RsaNI GTAC 3 cut(s) 517, 674, 1110
RseI CAYNNNNRTG 1 cut(s) 574
SaqAI TTAA 8 cut(s) 63, 239, 321, 414, 623, 759, 764, 902
SatI GCNGC 2 cut(s) 705, 721
Sau3AI GATC 4 cut(s) 50, 100, 558, 849
Sau96I GGNCC 3 cut(s) 250, 356, 419
ScaI AGTACT 2 cut(s) 675, 1111
SchI GAGTC 2 cut(s) 103, 1333
ScrFI CCNGG 2 cut(s) 1126, 1219
SduI GDGCHC 1 cut(s) 701
SfaNI GCATC 3 cut(s) 578, 1104, 1312
SfcI CTRYAG 1 cut(s) 24
SinI GGWCC 3 cut(s) 250, 356, 419
SmiMI CAYNNNNRTG 1 cut(s) 574
SmlI CTYRAG 3 cut(s) 220, 413, 463
SmoI CTYRAG 3 cut(s) 220, 413, 463
SspMI CTAG 5 cut(s) 350, 483, 830, 875, 1047
StyD4I CCNGG 2 cut(s) 1124, 1217
TaaI ACNGT 1 cut(s) 523
TaiI ACGT 2 cut(s) 1080, 1245
TaqI TCGA 2 cut(s) 11, 31
TatI WGTACW 3 cut(s) 516, 673, 1109
TfiI GAWTC 3 cut(s) 268, 634, 1042
Tru1I TTAA 8 cut(s) 63, 239, 321, 414, 623, 759, 764, 902
Tru9I TTAA 8 cut(s) 63, 239, 321, 414, 623, 759, 764, 902
TscAI CASTG 4 cut(s) 526, 647, 1267, 1308
TseFI GTSAC 1 cut(s) 1073
TseI GCWGC 2 cut(s) 704, 720
Tsp45I GTSAC 1 cut(s) 1073
TspDTI ATGAA 6 cut(s) 81, 260, 389, 1055, 1310, 1326
TspGWI ACGGA 2 cut(s) 927, 1073
TspRI CASTG 4 cut(s) 526, 647, 1267, 1308
Van91I CCANNNNNTGG 2 cut(s) 1025, 1280
Vha464I CTTAAG 1 cut(s) 413
VpaK11BI GGWCC 3 cut(s) 250, 356, 419
XapI RAATTY 1 cut(s) 1172
XbaI TCTAGA 2 cut(s) 349, 829
XmiI GTMKAC 1 cut(s) 820
XspI CTAG 5 cut(s) 350, 483, 830, 875, 1047
ZrmI AGTACT 2 cut(s) 675, 1111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.