MD12G1173900.v1.1

Receptor-like protein 12

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0016941
Physical Location & Seq
Forward (+)
25404120 .. 25404644
525 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1173900.v1.1.491

Sequence Viewer

Length: 525 bp
ATGCTTCCACCATCTGCCTATTATCTGGATTACTCAAGAAATAATTTCAGCTCAAGCATACCGGCAGACATTGGTGATTTCCTTGATTGGACAGTGTTCTTCTCTCTTGCAAGTAATCACATCCACGGGATCATTCCAGAATCAGTATGCAACGTGACAGTTCTTCTAGTTCCTGATCTGTCCAATAATTCCTTAAGTGGCATGATTCTCCAGTGCTTAACTGCAGGGAACATGTCCCTTGCAGTACTTAATTTAAGGAGAAACAAACTTTTTGGCACTCTTCCTGATAATTTTCCTGAGCACTGTAGTCTACAAACACTGGACCTCAATGGCAATGTGATTGGTGGTCAGTTCCCAAAATCTCTAGCCAACTGCACAATGTTAGAGGCGTTAAACCTGGGAAACAATCAAATAACAGATGTCTTTCCATGCTTATTGAAGAACATATCCAGCTTGCGTGTACTTGTGTTGCGTGTCAACAAATTTTATGATCGCATTGAATGCCTCAAGACCAATAGTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009888 GO:0010073 GO:0032501 GO:0032502 GO:0048507 GO:0048856
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8

Protein Analysis

175

Amino Acids

19.18

Weight (kDa)

5.83

Isoelectric Point (pI)

52.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 103 - 161 3.6e-07 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 310
AclWI GGATC 1 cut(s) 137
AcsI RAATTY 1 cut(s) 482
AfaI GTAC 2 cut(s) 246, 462
AflII CTTAAG 1 cut(s) 193
AflIII ACRYGT 1 cut(s) 231
AgsI TTSAA 2 cut(s) 439, 500
AjnI CCWGG 1 cut(s) 396
AluBI AGCT 2 cut(s) 51, 453
AluI AGCT 2 cut(s) 51, 453
Alw21I GWGCWC 1 cut(s) 303
AlwI GGATC 1 cut(s) 137
ApoI RAATTY 1 cut(s) 482
AspS9I GGNCC 1 cut(s) 322
AsuHPI GGTGA 1 cut(s) 86
AvaII GGWCC 1 cut(s) 322
Bbv12I GWGCWC 1 cut(s) 303
BccI CCATC 1 cut(s) 19
BciT130I CCWGG 1 cut(s) 398
BfaI CTAG 2 cut(s) 167, 365
BfmI CTRYAG 2 cut(s) 222, 304
BfrI CTTAAG 1 cut(s) 193
BmcAI AGTACT 1 cut(s) 246
Bme1390I CCNGG 1 cut(s) 398
Bme18I GGWCC 1 cut(s) 322
BmgT120I GGNCC 1 cut(s) 322
BmrFI CCNGG 1 cut(s) 398
BpmI CTGGAG 1 cut(s) 194
Bpu10I CCTNAGC 1 cut(s) 297
BpuEI CTTGAG 3 cut(s) 19, 37, 491
BsaJI CCNNGG 2 cut(s) 124, 397
Bse118I RCCGGY 1 cut(s) 61
Bse1I ACTGG 2 cut(s) 211, 324
Bse3DI GCAATG 1 cut(s) 340
BseBI CCWGG 1 cut(s) 398
BseDI CCNNGG 2 cut(s) 124, 397
BseGI GGATG 1 cut(s) 120
BseMI GCAATG 1 cut(s) 340
BseMII CTCAG 1 cut(s) 288
BseNI ACTGG 2 cut(s) 211, 324
BsgI GTGCAG 1 cut(s) 358
BsiHKAI GWGCWC 1 cut(s) 303
BsiSI CCGG 1 cut(s) 62
BslFI GGGAC 1 cut(s) 220
BsmFI GGGAC 1 cut(s) 220
BsmI GAATGC 1 cut(s) 506
Bsp1286I GDGCHC 1 cut(s) 303
Bsp143I GATC 3 cut(s) 129, 175, 490
BspCNI CTCAG 1 cut(s) 289
BspMAI CTGCAG 1 cut(s) 226
BspPI GGATC 1 cut(s) 137
BspTI CTTAAG 1 cut(s) 193
BsrDI GCAATG 1 cut(s) 340
BsrFI RCCGGY 1 cut(s) 61
BsrI ACTGG 2 cut(s) 211, 324
BssAI RCCGGY 1 cut(s) 61
BssECI CCNNGG 2 cut(s) 124, 397
BssMI GATC 3 cut(s) 129, 175, 490
Bst2UI CCWGG 1 cut(s) 398
Bst4CI ACNGT 3 cut(s) 94, 160, 305
Bst6I CTCTTC 1 cut(s) 285
BstAFI CTTAAG 1 cut(s) 193
BstAPI GCANNNNNTGC 1 cut(s) 501
BstC8I GCNNGC 1 cut(s) 455
BstDEI CTNAG 1 cut(s) 297
BstDSI CCRYGG 1 cut(s) 124
BstF5I GGATG 1 cut(s) 120
BstKTI GATC 3 cut(s) 132, 178, 493
BstMBI GATC 3 cut(s) 129, 175, 490
BstMWI GCNNNNNNNGC 1 cut(s) 501
BstNI CCWGG 1 cut(s) 398
BstNSI RCATGY 1 cut(s) 235
BstSCI CCNGG 1 cut(s) 396
BstSFI CTRYAG 2 cut(s) 222, 304
BtgI CCRYGG 1 cut(s) 124
BtsCI GGATG 1 cut(s) 120
BtsIMutI CAGTG 4 cut(s) 99, 218, 301, 317
Cac8I GCNNGC 1 cut(s) 455
Cfr10I RCCGGY 1 cut(s) 61
Cfr13I GGNCC 1 cut(s) 322
Csp6I GTAC 2 cut(s) 245, 461
CviAII CATG 3 cut(s) 202, 232, 429
CviJI RGCY 3 cut(s) 51, 368, 453
CviKI_1 RGCY 3 cut(s) 51, 368, 453
CviQI GTAC 2 cut(s) 245, 461
DdeI CTNAG 1 cut(s) 297
DpnI GATC 3 cut(s) 131, 177, 492
DpnII GATC 3 cut(s) 129, 175, 490
Eam1104I CTCTTC 1 cut(s) 285
EarI CTCTTC 1 cut(s) 285
Eco47I GGWCC 1 cut(s) 322
EcoRII CCWGG 1 cut(s) 396
FaeI CATG 3 cut(s) 205, 235, 432
FaiI YATR 7 cut(s) 59, 148, 203, 233, 430, 446, 489
FaqI GGGAC 1 cut(s) 220
FatI CATG 3 cut(s) 201, 231, 428
FblI GTMKAC 1 cut(s) 310
FokI GGATG 1 cut(s) 107
FspBI CTAG 2 cut(s) 167, 365
GsuI CTGGAG 1 cut(s) 194
HapII CCGG 1 cut(s) 62
Hin1II CATG 3 cut(s) 205, 235, 432
HincII GTYRAC 1 cut(s) 478
HindII GTYRAC 1 cut(s) 478
HinfI GANTC 2 cut(s) 140, 205
HpaII CCGG 1 cut(s) 62
HphI GGTGA 1 cut(s) 86
Hpy166II GTNNAC 3 cut(s) 311, 461, 478
Hpy188III TCNNGA 7 cut(s) 26, 36, 137, 173, 284, 296, 508
Hpy8I GTNNAC 3 cut(s) 311, 461, 478
HpyCH4III ACNGT 3 cut(s) 94, 160, 305
HpyCH4IV ACGT 1 cut(s) 153
HpyCH4V TGCA 5 cut(s) 110, 150, 224, 242, 375
HpyF10VI GCNNNNNNNGC 1 cut(s) 501
HpyF3I CTNAG 1 cut(s) 297
HpySE526I ACGT 1 cut(s) 153
Hsp92II CATG 3 cut(s) 205, 235, 432
Kzo9I GATC 3 cut(s) 129, 175, 490
MaeI CTAG 2 cut(s) 167, 365
MaeII ACGT 1 cut(s) 153
MaeIII GTNAC 1 cut(s) 154
MalI GATC 3 cut(s) 131, 177, 492
MboI GATC 3 cut(s) 129, 175, 490
MboII GAAGA 4 cut(s) 91, 155, 272, 451
MhlI GDGCHC 1 cut(s) 303
MluCI AATT 5 cut(s) 43, 187, 250, 289, 482
MnlI CCTC 3 cut(s) 335, 379, 515
MseI TTAA 5 cut(s) 194, 218, 249, 254, 392
MspCI CTTAAG 1 cut(s) 193
MspI CCGG 1 cut(s) 62
MspR9I CCNGG 1 cut(s) 398
Mva1269I GAATGC 1 cut(s) 506
MvaI CCWGG 1 cut(s) 398
MwoI GCNNNNNNNGC 1 cut(s) 501
NdeII GATC 3 cut(s) 129, 175, 490
NlaIII CATG 3 cut(s) 205, 235, 432
NmuCI GTSAC 1 cut(s) 154
NspI RCATGY 1 cut(s) 235
PciI ACATGT 1 cut(s) 231
PctI GAATGC 1 cut(s) 506
PfeI GAWTC 2 cut(s) 140, 205
PscI ACATGT 1 cut(s) 231
Psp6I CCWGG 1 cut(s) 396
PspGI CCWGG 1 cut(s) 396
PspPI GGNCC 1 cut(s) 322
PstI CTGCAG 1 cut(s) 226
RsaI GTAC 2 cut(s) 246, 462
RsaNI GTAC 2 cut(s) 245, 461
SaqAI TTAA 5 cut(s) 194, 218, 249, 254, 392
Sau3AI GATC 3 cut(s) 129, 175, 490
Sau96I GGNCC 1 cut(s) 322
ScaI AGTACT 1 cut(s) 246
ScrFI CCNGG 1 cut(s) 398
SduI GDGCHC 1 cut(s) 303
SetI ASST 5 cut(s) 53, 156, 327, 399, 455
SfcI CTRYAG 2 cut(s) 222, 304
SinI GGWCC 1 cut(s) 322
SmlI CTYRAG 4 cut(s) 34, 52, 193, 506
SmoI CTYRAG 4 cut(s) 34, 52, 193, 506
Sse9I AATT 5 cut(s) 43, 187, 250, 289, 482
SspMI CTAG 2 cut(s) 167, 365
StyD4I CCNGG 1 cut(s) 396
TaaI ACNGT 3 cut(s) 94, 160, 305
TaiI ACGT 1 cut(s) 156
TasI AATT 5 cut(s) 43, 187, 250, 289, 482
TatI WGTACW 2 cut(s) 244, 460
TfiI GAWTC 2 cut(s) 140, 205
Tru1I TTAA 5 cut(s) 194, 218, 249, 254, 392
Tru9I TTAA 5 cut(s) 194, 218, 249, 254, 392
TscAI CASTG 4 cut(s) 99, 218, 308, 324
TseFI GTSAC 1 cut(s) 154
Tsp45I GTSAC 1 cut(s) 154
TspRI CASTG 4 cut(s) 99, 218, 308, 324
Vha464I CTTAAG 1 cut(s) 193
VpaK11BI GGWCC 1 cut(s) 322
XapI RAATTY 1 cut(s) 482
XceI RCATGY 1 cut(s) 235
XmiI GTMKAC 1 cut(s) 310
XspI CTAG 2 cut(s) 167, 365
ZrmI AGTACT 1 cut(s) 246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.