MD12G1174300.v1.1

Receptor-like protein 12

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0016941
Physical Location & Seq
Forward (+)
25453166 .. 25453774
609 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1174300.v1.1.491

Sequence Viewer

Length: 609 bp
ATGTACACTGTGTTCTTCTCTCTTGCAAGCAATCACATCCACGGGATCATTCCAGAATCAGTATGCAAGGCGACATCTCTTCATGTTCTTGATATGTCCAATAATTCCTTTAGTGGCATGATTCCCCAGTGCTTAACTGCAAGGAACAGGTCTCTTGCAGTAATTAATTTAAGGAGAAACAAACTTTCTCGCACTCTTCCTGATAATTTTCCTCAGCACTGTAGTCTACGAAGTTTAGACCTCCATGGCAATGTGATTGGTGGTCAGTTCCCAAAATCTCTAGCCAATTGCACAATGTTAGAGGTGTTAAACCTGGGAAACAATCAGATAACAAATGTCTTTCCGTGCTTATTGATGAACATATCCAGCTTGCGTGTACTTGTCTTGCGTTCCAACAAATTTCATGATCGCATTGAATGCCTCAGCCTCAAGACCAATGGTCATTGGCCAAAGCTTCCAGTTATTGACATAGCTCACAACAATTTTAGTGGTGAACTACCAGGAAGATGCTGGAGAACCTGGCTAGCAATGATGGCTGACGAAGATGATGCCGAGTTTAAAATTGCCTACGACATCTTGAGTCCTTGGACCTTTCGGACAACAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009888 GO:0010073 GO:0032501 GO:0032502 GO:0048507 GO:0048856
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8

Protein Analysis

203

Amino Acids

22.84

Weight (kDa)

8.53

Isoelectric Point (pI)

51.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 75 - 134 2.8e-08 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 226
AclWI GGATC 1 cut(s) 53
AcoI YGGCCR 1 cut(s) 446
AcsI RAATTY 1 cut(s) 398
AfaI GTAC 2 cut(s) 5, 378
AgsI TTSAA 1 cut(s) 416
AhdI GACNNNNNGTC 1 cut(s) 438
AjnI CCWGG 3 cut(s) 312, 499, 518
AluBI AGCT 3 cut(s) 369, 454, 473
AluI AGCT 3 cut(s) 369, 454, 473
Alw26I GTCTC 1 cut(s) 156
AlwI GGATC 1 cut(s) 53
AoxI GGCC 1 cut(s) 446
ApoI RAATTY 1 cut(s) 398
AseI ATTAAT 1 cut(s) 165
AspS9I GGNCC 1 cut(s) 588
AsuHPI GGTGA 1 cut(s) 503
AsuNHI GCTAGC 1 cut(s) 523
AvaII GGWCC 1 cut(s) 588
BalI TGGCCA 1 cut(s) 448
BbvCI CCTCAGC 2 cut(s) 213, 422
BccI CCATC 1 cut(s) 526
BcgI CGANNNNNNTGC 2 cut(s) 530, 564
BciT130I CCWGG 3 cut(s) 314, 501, 520
BcoDI GTCTC 1 cut(s) 156
BfaI CTAG 2 cut(s) 281, 524
BfmI CTRYAG 1 cut(s) 220
Bme1390I CCNGG 3 cut(s) 314, 501, 520
Bme18I GGWCC 1 cut(s) 588
BmeRI GACNNNNNGTC 1 cut(s) 438
BmgT120I GGNCC 1 cut(s) 588
BmrFI CCNGG 3 cut(s) 314, 501, 520
BmrI ACTGGG 1 cut(s) 121
BmsI GCATC 2 cut(s) 497, 538
BmtI GCTAGC 1 cut(s) 527
BmuI ACTGGG 1 cut(s) 121
BpmI CTGGAG 1 cut(s) 532
Bpu10I CCTNAGC 2 cut(s) 213, 422
BpuEI CTTGAG 2 cut(s) 413, 598
BsaI GGTCTC 1 cut(s) 156
BsaJI CCNNGG 4 cut(s) 40, 244, 313, 584
Bse1I ACTGG 2 cut(s) 127, 458
Bse3DI GCAATG 2 cut(s) 256, 534
BseBI CCWGG 3 cut(s) 314, 501, 520
BseDI CCNNGG 4 cut(s) 40, 244, 313, 584
BseGI GGATG 1 cut(s) 36
BseMI GCAATG 2 cut(s) 256, 534
BseMII CTCAG 2 cut(s) 227, 436
BseNI ACTGG 2 cut(s) 127, 458
BshFI GGCC 1 cut(s) 448
BsmAI GTCTC 1 cut(s) 156
BsmI GAATGC 1 cut(s) 422
BsnI GGCC 1 cut(s) 448
Bso31I GGTCTC 1 cut(s) 156
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 2 cut(s) 45, 406
Bsp19I CCATGG 1 cut(s) 244
BspANI GGCC 1 cut(s) 448
BspCNI CTCAG 2 cut(s) 226, 435
BspHI TCATGA 1 cut(s) 403
BspOI GCTAGC 1 cut(s) 527
BspPI GGATC 1 cut(s) 53
BspTNI GGTCTC 1 cut(s) 156
BsrDI GCAATG 2 cut(s) 256, 534
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 2 cut(s) 127, 458
BssECI CCNNGG 4 cut(s) 40, 244, 313, 584
BssMI GATC 2 cut(s) 45, 406
BssT1I CCWWGG 2 cut(s) 244, 584
Bst2UI CCWGG 3 cut(s) 314, 501, 520
Bst4CI ACNGT 2 cut(s) 10, 221
Bst6I CTCTTC 2 cut(s) 84, 201
BstAPI GCANNNNNTGC 1 cut(s) 417
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 3 cut(s) 28, 371, 525
BstDEI CTNAG 2 cut(s) 213, 422
BstDSI CCRYGG 2 cut(s) 40, 244
BstF5I GGATG 1 cut(s) 36
BstKTI GATC 2 cut(s) 48, 409
BstMAI GTCTC 1 cut(s) 156
BstMBI GATC 2 cut(s) 45, 406
BstMWI GCNNNNNNNGC 2 cut(s) 417, 533
BstNI CCWGG 3 cut(s) 314, 501, 520
BstSCI CCNGG 3 cut(s) 312, 499, 518
BstSFI CTRYAG 1 cut(s) 220
BsuRI GGCC 1 cut(s) 448
BtgI CCRYGG 2 cut(s) 40, 244
BtsCI GGATG 1 cut(s) 36
BtsIMutI CAGTG 3 cut(s) 6, 134, 217
Cac8I GCNNGC 3 cut(s) 28, 371, 525
CciI TCATGA 1 cut(s) 403
Cfr13I GGNCC 1 cut(s) 588
Csp6I GTAC 2 cut(s) 4, 377
CspCI CAANNNNNGTGG 2 cut(s) 469, 504
CviAII CATG 4 cut(s) 83, 118, 245, 404
CviJI RGCY 8 cut(s) 284, 369, 426, 448, 454, 473, 523, 536
CviKI_1 RGCY 8 cut(s) 284, 369, 426, 448, 454, 473, 523, 536
CviQI GTAC 2 cut(s) 4, 377
DdeI CTNAG 2 cut(s) 213, 422
DpnI GATC 2 cut(s) 47, 408
DpnII GATC 2 cut(s) 45, 406
DraI TTTAAA 1 cut(s) 559
DriI GACNNNNNGTC 1 cut(s) 438
EaeI YGGCCR 1 cut(s) 446
Eam1104I CTCTTC 2 cut(s) 84, 201
Eam1105I GACNNNNNGTC 1 cut(s) 438
EarI CTCTTC 2 cut(s) 84, 201
Eco130I CCWWGG 2 cut(s) 244, 584
Eco31I GGTCTC 1 cut(s) 156
Eco47I GGWCC 1 cut(s) 588
EcoRII CCWGG 3 cut(s) 312, 499, 518
EcoT14I CCWWGG 2 cut(s) 244, 584
ErhI CCWWGG 2 cut(s) 244, 584
FaeI CATG 4 cut(s) 86, 121, 248, 407
FaiI YATR 8 cut(s) 64, 84, 95, 119, 246, 362, 405, 470
FatI CATG 4 cut(s) 82, 117, 244, 403
FblI GTMKAC 1 cut(s) 226
FokI GGATG 1 cut(s) 23
FspBI CTAG 2 cut(s) 281, 524
GsuI CTGGAG 1 cut(s) 532
HaeIII GGCC 1 cut(s) 448
Hin1II CATG 4 cut(s) 86, 121, 248, 407
HindIII AAGCTT 1 cut(s) 452
HinfI GANTC 3 cut(s) 56, 121, 580
HphI GGTGA 1 cut(s) 503
Hpy166II GTNNAC 4 cut(s) 6, 227, 377, 494
Hpy188I TCNGA 2 cut(s) 327, 597
Hpy188III TCNNGA 6 cut(s) 53, 89, 200, 404, 430, 577
Hpy8I GTNNAC 4 cut(s) 6, 227, 377, 494
HpyCH4III ACNGT 2 cut(s) 10, 221
HpyCH4V TGCA 5 cut(s) 26, 66, 140, 158, 291
HpyF10VI GCNNNNNNNGC 2 cut(s) 417, 533
HpyF3I CTNAG 2 cut(s) 213, 422
Hsp92II CATG 4 cut(s) 86, 121, 248, 407
Kzo9I GATC 2 cut(s) 45, 406
LweI GCATC 2 cut(s) 497, 538
MaeI CTAG 2 cut(s) 281, 524
MalI GATC 2 cut(s) 47, 408
MboI GATC 2 cut(s) 45, 406
MboII GAAGA 5 cut(s) 7, 71, 188, 516, 554
MfeI CAATTG 1 cut(s) 286
MlsI TGGCCA 1 cut(s) 448
MluCI AATT 8 cut(s) 103, 162, 166, 205, 286, 398, 481, 561
MluNI TGGCCA 1 cut(s) 448
MlyI GAGTC 1 cut(s) 589
MmeI TCCRAC 1 cut(s) 417
MnlI CCTC 5 cut(s) 222, 251, 295, 431, 437
Mox20I TGGCCA 1 cut(s) 448
MscI TGGCCA 1 cut(s) 448
MseI TTAA 5 cut(s) 134, 165, 170, 308, 558
MslI CAYNNNNRTG 1 cut(s) 249
Msp20I TGGCCA 1 cut(s) 448
MspR9I CCNGG 3 cut(s) 314, 501, 520
MunI CAATTG 1 cut(s) 286
Mva1269I GAATGC 1 cut(s) 422
MvaI CCWGG 3 cut(s) 314, 501, 520
MwoI GCNNNNNNNGC 2 cut(s) 417, 533
NcoI CCATGG 1 cut(s) 244
NdeII GATC 2 cut(s) 45, 406
NheI GCTAGC 1 cut(s) 523
NlaIII CATG 4 cut(s) 86, 121, 248, 407
NmeAIII GCCGAG 1 cut(s) 577
PagI TCATGA 1 cut(s) 403
PctI GAATGC 1 cut(s) 422
PfeI GAWTC 2 cut(s) 56, 121
PleI GAGTC 1 cut(s) 588
PpsI GAGTC 1 cut(s) 588
PshBI ATTAAT 1 cut(s) 165
Psp6I CCWGG 3 cut(s) 312, 499, 518
PspGI CCWGG 3 cut(s) 312, 499, 518
PspPI GGNCC 1 cut(s) 588
PsrI GAACNNNNNNTAC 1 cut(s) 28
RsaI GTAC 2 cut(s) 5, 378
RsaNI GTAC 2 cut(s) 4, 377
RseI CAYNNNNRTG 1 cut(s) 249
SaqAI TTAA 5 cut(s) 134, 165, 170, 308, 558
Sau3AI GATC 2 cut(s) 45, 406
Sau96I GGNCC 1 cut(s) 588
SchI GAGTC 1 cut(s) 589
ScrFI CCNGG 3 cut(s) 314, 501, 520
SetI ASST 9 cut(s) 152, 243, 306, 315, 371, 456, 475, 521, 593
SfaNI GCATC 2 cut(s) 497, 538
SfcI CTRYAG 1 cut(s) 220
SinI GGWCC 1 cut(s) 588
SmiMI CAYNNNNRTG 1 cut(s) 249
SmlI CTYRAG 2 cut(s) 428, 577
SmoI CTYRAG 2 cut(s) 428, 577
Sse9I AATT 8 cut(s) 103, 162, 166, 205, 286, 398, 481, 561
SspMI CTAG 2 cut(s) 281, 524
StyD4I CCNGG 3 cut(s) 312, 499, 518
StyI CCWWGG 2 cut(s) 244, 584
TaaI ACNGT 2 cut(s) 10, 221
TasI AATT 8 cut(s) 103, 162, 166, 205, 286, 398, 481, 561
TatI WGTACW 2 cut(s) 3, 376
TfiI GAWTC 2 cut(s) 56, 121
Tru1I TTAA 5 cut(s) 134, 165, 170, 308, 558
Tru9I TTAA 5 cut(s) 134, 165, 170, 308, 558
TscAI CASTG 3 cut(s) 13, 134, 224
TspDTI ATGAA 3 cut(s) 71, 371, 392
TspGWI ACGGA 1 cut(s) 333
TspRI CASTG 3 cut(s) 13, 134, 224
VpaK11BI GGWCC 1 cut(s) 588
VspI ATTAAT 1 cut(s) 165
XapI RAATTY 1 cut(s) 398
XcmI CCANNNNNNNNNTGG 1 cut(s) 507
XmiI GTMKAC 1 cut(s) 226
XspI CTAG 2 cut(s) 281, 524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.