MD12G1161100.v1.1
MYB Family

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0012757
Physical Location & Seq
Forward (+)
24109690 .. 24112039
2350 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1161100.v1.1.491

Sequence Viewer

Length: 915 bp
ATGTTCAGAGGAATGGAATTTTTCTCTCCAAATCTGCAAAACTCCAATTGGTTGTTTCAAGAGAGCAAGGGAGCCGATTGGACTGCAGAAGAGAACAAGCTATTCGAAAATGCTCTCGCTTTATACGATAAGGACACGCCCGAGCGGTGGCTCAGTGTGGCGGCTATGATCCCCGGAAAGACTGTCGGAGATGTCATCAAACAGTACAAGGAATTAGAAGAAGATGTCAGTGATATCGAAGCCGGGCTTATCCCAATTCCTGGTTACACCAGTAACAATTTCACATTGGAGTGGGTTAACGATCAGGGATTTGACGGGTTGAAGCAGTTTTACAGTGTTGGTGGAAAGCGAGGTGCCTCGACTAGGCCCGCTGATCAGGAGAGAAAGAAAGGGGTGCCATGGACAGAAGAAGAACACAGGCAATTTCTGATGGGCCTTAAAAAGTATGGAAAAGGTGACTGGAGAAACATCTCTCGAAATTTTGTGACCACAAGGACGCCCACTCAGGTTGCTAGTCATGCACAAAAGTACTTTATCAGGCAACTCACAGGAGGGAAGGACAAGAGAAGATCTAGCATCCACGATATCACAACAGTCAATCTCCTCGATGTGAAACCGTCCTCACCTGACAGTACTAAGAGCCCGCCATCATCGGCTCATTCCTCAACCGTGGTGCAGCAGCCACACCAGCATCAGAAGTTGACCGGCACGAGCATTGACTGGAAATCAGCCGATGAGGGACAGCATTTGGTTGGTTCTGCAAATGGGAACAACAACTTCATGGGACCGTTTTGTGGGATGTCTACACACGTACCTAAGCTTGAGGAGCAAAGTTTTCTGAGTGGCAGTCTTCATGGATCTCAGTTTGGACATTACAATGCGAGGTTTGAGATGCAATCAATGCGATACCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

305

Amino Acids

34.27

Weight (kDa)

7.17

Isoelectric Point (pI)

42.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 26 - 70 4.3e-06 Myb-like DNA-binding domain
Myb_DNA-binding_2 PF23082 26 - 74 8.5e-08 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 132 - 178 8.6e-13 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012757)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G38090
fragaria_vesca FvH4_6g11950
malus_domestica MD04G1147800.v1.1 MD12G1161100.v1.1
prunus_persica Prupe.6G271600_v2.0.a1
pyrus_communis pycom04g13340 pycom12g15290
rosa_chinensis RchiOBHm_Chr3g0463761
rosa_laevigata RLG00000024759
rosa_multiflora Rmu_sc0000142.1_g000001 Rmu_sc0003267.1_g000043
rosa_roxburghii Rroxscaffold_6G00416330
rosa_rugosa Rorug03G0067300
rosa_samantha Rh3AG124500 Rh3BG129100 Rh3CG131400 Rh3DG129700
rosa_wichuraiana Rw3G010520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 353, 394
AccB7I CCANNNNNTGG 1 cut(s) 260
AccBSI CCGCTC 1 cut(s) 145
AccI GTMKAC 1 cut(s) 803
AciI CCGC 4 cut(s) 145, 161, 369, 644
AclWI GGATC 2 cut(s) 163, 865
AcsI RAATTY 2 cut(s) 17, 478
AcyI GRCGYC 1 cut(s) 497
AfaI GTAC 4 cut(s) 206, 530, 634, 813
AfiI CCNNNNNNNGG 4 cut(s) 147, 260, 363, 794
AflIII ACRYGT 1 cut(s) 808
AgsI TTSAA 2 cut(s) 59, 322
AjnI CCWGG 1 cut(s) 259
AluBI AGCT 2 cut(s) 100, 820
AluI AGCT 2 cut(s) 100, 820
AlwI GGATC 2 cut(s) 163, 865
Ama87I CYCGRG 1 cut(s) 140
AoxI GGCC 2 cut(s) 365, 433
ApeKI GCWGC 2 cut(s) 676, 679
ApoI RAATTY 2 cut(s) 17, 478
AspS9I GGNCC 3 cut(s) 366, 433, 785
AsuC2I CCSGG 2 cut(s) 174, 244
AsuHPI GGTGA 2 cut(s) 467, 615
AsuII TTCGAA 1 cut(s) 105
AvaI CYCGRG 1 cut(s) 140
AvaII GGWCC 1 cut(s) 785
BaeI ACNNNNGTAYC 2 cut(s) 795, 828
BanI GGYRCC 2 cut(s) 353, 394
BanII GRGCYC 1 cut(s) 644
BarI GAAGNNNNNNTAC 2 cut(s) 314, 346
BauI CACGAG 1 cut(s) 709
BbsI GAAGAC 1 cut(s) 842
BbvI GCAGC 2 cut(s) 688, 691
BccI CCATC 2 cut(s) 424, 655
BcgI CGANNNNNNTGC 2 cut(s) 65, 99
BciT130I CCWGG 1 cut(s) 261
BclI TGATCA 1 cut(s) 373
BcnI CCSGG 2 cut(s) 174, 244
BfaI CTAG 3 cut(s) 363, 513, 573
BfmI CTRYAG 1 cut(s) 84
BglII AGATCT 1 cut(s) 569
BisI GCNGC 3 cut(s) 162, 677, 680
BlsI GCNGC 3 cut(s) 163, 678, 681
BmcAI AGTACT 2 cut(s) 530, 634
Bme1390I CCNGG 3 cut(s) 174, 244, 261
Bme18I GGWCC 1 cut(s) 785
BmeT110I CYCGRG 1 cut(s) 140
BmgT120I GGNCC 3 cut(s) 366, 433, 785
BmiI GGNNCC 4 cut(s) 73, 355, 396, 786
BmrFI CCNGG 3 cut(s) 174, 244, 261
BmsI GCATC 3 cut(s) 585, 700, 882
BpiI GAAGAC 1 cut(s) 842
BpmI CTGGAG 1 cut(s) 481
Bpu10I CCTNAGC 1 cut(s) 816
Bpu14I TTCGAA 1 cut(s) 105
BpuEI CTTGAG 1 cut(s) 842
BpuMI CCSGG 2 cut(s) 174, 244
BsaAI YACGTR 1 cut(s) 811
BsaHI GRCGYC 1 cut(s) 497
BsaJI CCNNGG 3 cut(s) 172, 398, 669
Bsc4I CCNNNNNNNGG 4 cut(s) 147, 260, 363, 794
Bse118I RCCGGY 1 cut(s) 704
Bse1I ACTGG 4 cut(s) 270, 464, 725, 910
BseBI CCWGG 1 cut(s) 261
BseDI CCNNGG 3 cut(s) 172, 398, 669
BseGI GGATG 2 cut(s) 576, 804
BseLI CCNNNNNNNGG 4 cut(s) 147, 260, 363, 794
BseMII CTCAG 4 cut(s) 166, 518, 830, 875
BseNI ACTGG 4 cut(s) 270, 464, 725, 910
BseRI GAGGAG 2 cut(s) 593, 839
BseXI GCAGC 2 cut(s) 688, 691
BsgI GTGCAG 1 cut(s) 695
BshFI GGCC 2 cut(s) 367, 435
BshNI GGYRCC 2 cut(s) 353, 394
BsiHKCI CYCGRG 1 cut(s) 140
BsiSI CCGG 3 cut(s) 174, 243, 705
BslFI GGGAC 2 cut(s) 753, 798
BslI CCNNNNNNNGG 4 cut(s) 147, 260, 363, 794
BsmFI GGGAC 2 cut(s) 753, 798
BsnI GGCC 2 cut(s) 367, 435
BsoBI CYCGRG 1 cut(s) 140
Bsp119I TTCGAA 1 cut(s) 105
Bsp1286I GDGCHC 1 cut(s) 644
Bsp143I GATC 5 cut(s) 168, 301, 373, 569, 857
Bsp19I CCATGG 1 cut(s) 398
BspACI CCGC 4 cut(s) 145, 161, 369, 644
BspANI GGCC 2 cut(s) 367, 435
BspCNI CTCAG 4 cut(s) 165, 517, 831, 874
BspLI GGNNCC 4 cut(s) 73, 355, 396, 786
BspMAI CTGCAG 1 cut(s) 88
BspPI GGATC 2 cut(s) 163, 865
BspT104I TTCGAA 1 cut(s) 105
BspT107I GGYRCC 2 cut(s) 353, 394
BsrBI CCGCTC 1 cut(s) 145
BsrFI RCCGGY 1 cut(s) 704
BsrI ACTGG 4 cut(s) 270, 464, 725, 910
BssAI RCCGGY 1 cut(s) 704
BssECI CCNNGG 3 cut(s) 172, 398, 669
BssMI GATC 5 cut(s) 168, 301, 373, 569, 857
BssNI GRCGYC 1 cut(s) 497
BssSI CACGAG 1 cut(s) 709
BssT1I CCWWGG 1 cut(s) 398
Bst2BI CACGAG 1 cut(s) 709
Bst2UI CCWGG 1 cut(s) 261
Bst4CI ACNGT 8 cut(s) 184, 204, 335, 595, 618, 632, 670, 789
Bst6I CTCTTC 1 cut(s) 84
BstACI GRCGYC 1 cut(s) 497
BstAPI GCANNNNNTGC 1 cut(s) 901
BstBAI YACGTR 1 cut(s) 811
BstBI TTCGAA 1 cut(s) 105
BstC8I GCNNGC 2 cut(s) 369, 644
BstDEI CTNAG 6 cut(s) 152, 504, 636, 816, 839, 861
BstDSI CCRYGG 2 cut(s) 398, 669
BstENI CCTNNNNNAGG 1 cut(s) 361
BstF5I GGATG 2 cut(s) 576, 804
BstKTI GATC 5 cut(s) 171, 304, 376, 572, 860
BstMBI GATC 5 cut(s) 168, 301, 373, 569, 857
BstMWI GCNNNNNNNGC 4 cut(s) 518, 688, 826, 901
BstNI CCWGG 1 cut(s) 261
BstSCI CCNGG 3 cut(s) 172, 242, 259
BstSFI CTRYAG 1 cut(s) 84
BstV1I GCAGC 2 cut(s) 688, 691
BstV2I GAAGAC 1 cut(s) 842
BstX2I RGATCY 2 cut(s) 569, 857
BstYI RGATCY 2 cut(s) 569, 857
BsuRI GGCC 2 cut(s) 367, 435
BtgI CCRYGG 2 cut(s) 398, 669
BtsCI GGATG 2 cut(s) 576, 804
BtsIMutI CAGTG 3 cut(s) 160, 235, 340
Cac8I GCNNGC 2 cut(s) 369, 644
Cfr10I RCCGGY 1 cut(s) 704
Cfr13I GGNCC 3 cut(s) 366, 433, 785
CseI GACGC 1 cut(s) 505
Csp6I GTAC 4 cut(s) 205, 529, 633, 812
CspCI CAANNNNNGTGG 2 cut(s) 490, 525
CviAII CATG 4 cut(s) 399, 518, 781, 854
CviQI GTAC 4 cut(s) 205, 529, 633, 812
DdeI CTNAG 6 cut(s) 152, 504, 636, 816, 839, 861
DpnI GATC 5 cut(s) 170, 303, 375, 571, 859
DpnII GATC 5 cut(s) 168, 301, 373, 569, 857
Eam1104I CTCTTC 1 cut(s) 84
EarI CTCTTC 1 cut(s) 84
Eco130I CCWWGG 1 cut(s) 398
Eco24I GRGCYC 1 cut(s) 644
Eco32I GATATC 2 cut(s) 235, 586
Eco47I GGWCC 1 cut(s) 785
Eco88I CYCGRG 1 cut(s) 140
EcoNI CCTNNNNNAGG 1 cut(s) 361
EcoRII CCWGG 1 cut(s) 259
EcoRV GATATC 2 cut(s) 235, 586
EcoT14I CCWWGG 1 cut(s) 398
EcoT38I GRGCYC 1 cut(s) 644
ErhI CCWWGG 1 cut(s) 398
FaeI CATG 4 cut(s) 402, 521, 784, 857
FaiI YATR 7 cut(s) 124, 167, 400, 447, 519, 782, 855
FalI AAGNNNNNCTT 2 cut(s) 231, 263
FaqI GGGAC 2 cut(s) 753, 798
FatI CATG 4 cut(s) 398, 517, 780, 853
FauI CCCGC 2 cut(s) 376, 651
FbaI TGATCA 1 cut(s) 373
FblI GTMKAC 1 cut(s) 803
Fnu4HI GCNGC 3 cut(s) 162, 677, 680
FokI GGATG 2 cut(s) 563, 811
FriOI GRGCYC 1 cut(s) 644
Fsp4HI GCNGC 3 cut(s) 162, 677, 680
FspBI CTAG 3 cut(s) 363, 513, 573
GluI GCNGC 3 cut(s) 162, 677, 680
GsuI CTGGAG 1 cut(s) 481
HaeIII GGCC 2 cut(s) 367, 435
HapII CCGG 3 cut(s) 174, 243, 705
HgaI GACGC 1 cut(s) 505
Hin1I GRCGYC 1 cut(s) 497
Hin1II CATG 4 cut(s) 402, 521, 784, 857
HincII GTYRAC 2 cut(s) 298, 702
HindII GTYRAC 2 cut(s) 298, 702
HindIII AAGCTT 1 cut(s) 818
HpaI GTTAAC 1 cut(s) 298
HpaII CCGG 3 cut(s) 174, 243, 705
HphI GGTGA 2 cut(s) 467, 615
Hpy166II GTNNAC 3 cut(s) 298, 702, 804
Hpy188I TCNGA 5 cut(s) 8, 188, 429, 696, 840
Hpy188III TCNNGA 3 cut(s) 59, 377, 474
Hpy8I GTNNAC 3 cut(s) 298, 702, 804
HpyAV CCTTC 1 cut(s) 550
HpyCH4III ACNGT 8 cut(s) 184, 204, 335, 595, 618, 632, 670, 789
HpyCH4IV ACGT 1 cut(s) 810
HpyCH4V TGCA 6 cut(s) 37, 86, 521, 676, 761, 895
HpyF10VI GCNNNNNNNGC 4 cut(s) 518, 688, 826, 901
HpyF3I CTNAG 6 cut(s) 152, 504, 636, 816, 839, 861
HpySE526I ACGT 1 cut(s) 810
Hsp92I GRCGYC 1 cut(s) 497
Hsp92II CATG 4 cut(s) 402, 521, 784, 857
Ksp22I TGATCA 1 cut(s) 373
KspAI GTTAAC 1 cut(s) 298
Kzo9I GATC 5 cut(s) 168, 301, 373, 569, 857
LmnI GCTCC 2 cut(s) 71, 826
Lsp1109I GCAGC 2 cut(s) 688, 691
LweI GCATC 3 cut(s) 585, 700, 882
MaeI CTAG 3 cut(s) 363, 513, 573
MaeII ACGT 1 cut(s) 810
MaeIII GTNAC 4 cut(s) 263, 272, 455, 484
MalI GATC 5 cut(s) 170, 303, 375, 571, 859
MbiI CCGCTC 1 cut(s) 145
MboI GATC 5 cut(s) 168, 301, 373, 569, 857
MboII GAAGA 7 cut(s) 101, 230, 233, 419, 422, 579, 842
MfeI CAATTG 1 cut(s) 46
MflI RGATCY 2 cut(s) 569, 857
MhlI GDGCHC 1 cut(s) 644
MluCI AATT 7 cut(s) 17, 46, 212, 255, 277, 422, 478
MmeI TCCRAC 1 cut(s) 166
MnlI CCTC 9 cut(s) 344, 367, 545, 614, 631, 673, 730, 817, 876
MseI TTAA 2 cut(s) 297, 438
MslI CAYNNNNRTG 2 cut(s) 289, 876
MspA1I CMGCKG 1 cut(s) 371
MspI CCGG 3 cut(s) 174, 243, 705
MspR9I CCNGG 3 cut(s) 174, 244, 261
MunI CAATTG 1 cut(s) 46
MvaI CCWGG 1 cut(s) 261
MwoI GCNNNNNNNGC 4 cut(s) 518, 688, 826, 901
NciI CCSGG 2 cut(s) 174, 244
NcoI CCATGG 1 cut(s) 398
NdeII GATC 5 cut(s) 168, 301, 373, 569, 857
NlaIII CATG 4 cut(s) 402, 521, 784, 857
NlaIV GGNNCC 4 cut(s) 73, 355, 396, 786
NmuCI GTSAC 2 cut(s) 455, 484
NspV TTCGAA 1 cut(s) 105
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PflMI CCANNNNNTGG 1 cut(s) 260
PkrI GCNGC 3 cut(s) 163, 678, 681
Ppu21I YACGTR 1 cut(s) 811
Psp6I CCWGG 1 cut(s) 259
PspGI CCWGG 1 cut(s) 259
PspN4I GGNNCC 4 cut(s) 73, 355, 396, 786
PspPI GGNCC 3 cut(s) 366, 433, 785
PstI CTGCAG 1 cut(s) 88
PsuI RGATCY 2 cut(s) 569, 857
RsaI GTAC 4 cut(s) 206, 530, 634, 813
RsaNI GTAC 4 cut(s) 205, 529, 633, 812
RseI CAYNNNNRTG 2 cut(s) 289, 876
SaqAI TTAA 2 cut(s) 297, 438
SatI GCNGC 3 cut(s) 162, 677, 680
Sau3AI GATC 5 cut(s) 168, 301, 373, 569, 857
Sau96I GGNCC 3 cut(s) 366, 433, 785
ScaI AGTACT 2 cut(s) 530, 634
ScrFI CCNGG 3 cut(s) 174, 244, 261
SduI GDGCHC 1 cut(s) 644
SetI ASST 9 cut(s) 102, 355, 457, 510, 628, 813, 817, 822, 887
SfaNI GCATC 3 cut(s) 585, 700, 882
SfcI CTRYAG 1 cut(s) 84
SfuI TTCGAA 1 cut(s) 105
SinI GGWCC 1 cut(s) 785
SmiMI CAYNNNNRTG 2 cut(s) 289, 876
SmlI CTYRAG 1 cut(s) 821
SmoI CTYRAG 1 cut(s) 821
Sse9I AATT 7 cut(s) 17, 46, 212, 255, 277, 422, 478
SsiI CCGC 4 cut(s) 145, 161, 369, 644
SspMI CTAG 3 cut(s) 363, 513, 573
StyD4I CCNGG 3 cut(s) 172, 242, 259
StyI CCWWGG 1 cut(s) 398
TaaI ACNGT 8 cut(s) 184, 204, 335, 595, 618, 632, 670, 789
TaiI ACGT 1 cut(s) 813
TaqI TCGA 5 cut(s) 105, 237, 359, 475, 606
TasI AATT 7 cut(s) 17, 46, 212, 255, 277, 422, 478
TatI WGTACW 3 cut(s) 204, 528, 632
TauI GCSGC 1 cut(s) 164
Tru1I TTAA 2 cut(s) 297, 438
Tru9I TTAA 2 cut(s) 297, 438
TscAI CASTG 3 cut(s) 160, 235, 340
TseFI GTSAC 2 cut(s) 455, 484
TseI GCWGC 2 cut(s) 676, 679
Tsp45I GTSAC 2 cut(s) 455, 484
TspDTI ATGAA 2 cut(s) 769, 842
TspRI CASTG 3 cut(s) 160, 235, 340
Van91I CCANNNNNTGG 1 cut(s) 260
VpaK11BI GGWCC 1 cut(s) 785
XagI CCTNNNNNAGG 1 cut(s) 361
XapI RAATTY 2 cut(s) 17, 478
XmiI GTMKAC 1 cut(s) 803
XspI CTAG 3 cut(s) 363, 513, 573
ZrmI AGTACT 2 cut(s) 530, 634
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.