MD12G1181200.v1.1

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001664
Physical Location & Seq
Reverse (-)
26093938 .. 26097796
3859 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1181200.v1.1.491

Sequence Viewer

Length: 2016 bp
ATGAAGAGGCAAAGGACCATTCTACCCAACAACACCAACAACGACAACAACGTCTTCCATGTTCTTTCCGATGAAATCATATTCATAATCCTCGACTTCCTCGAAAAAAAACCAATTGACAAAAAGTCCTTCTCTTTGGTCTGCAAGTCCTTCCACGCCGCAGAATCCAAGCATCGCAAAAAGCTCAAGCCTTTGCGCTCCGACCACCTCGCGAGAGTCCTCCACCGCTACCCAAATGGCTCCCACGTCGATCTCACGCTCTGCTCGCGAGTCCCGGACGCTTCCCTCGTCGCCATCTCTAATGCCTGCAGGTCCACCCTCCGATCGATCGATCTTTCGCGGTCGAATTGCTTCTCGGGAATTGGATTGCTCAGTTTGGCTGCAAATTGCAAGAATCTGGTGGAGATCGACCTGTCGAACGCCACCGAGCTCAGAGACTCCGCGGTGGCGGCTCTGGCGGAGGCGAAGAATTTGGAGAGGCTGTGGATGGGGAGGTGCAAGATGATTACGGATATGGGTGTTGGGTGTATCGCAGTTGGTTGCAGAAAGCTGAGATTAATTAATTTGAAATGGTGCCTCCGTGTAAGCGACTTGGGGGTCGGATTGCTTGCTGTAAAGTGTAAAGATCTTCGAAGTTTGGATCTTTCTTACTTGCCGATCACAGATAGATGCTTGCCATCGATCTTCGAGTTACAATATCTTGAAGATTTGGTTCTAGAAGGATGCTTCAGTATCGATGATGATGGCCTTTCGGCCTTCAAACATGGGTGCAAGTCACTTAAGAAACTTGACATATCTAGCTGTCAGAACATTAGTCATGTTGGGTTGTCTGCCCTAACAAGCGGTAGTGACGGATGTTTAGAGCAACTCGTATTGTCATATGGCTCTCCTGTGACTCTTGCTCTTGCCGATAGCTTGGGAAAGCTTCCGACGTTGCAGTCAATCAAATTAGATGGTTGCCTGGTTACCTACGCTGGACTAAAAGCCATCGGAAACTGGTGCATTTCTCTAAGGGAGCTGAGCTTAAGTAAATGTGTGGGGGTGACTGATGAGGGTCTCTCCTCTATTTTGAAAAAGCACAAGAATTTGCAGAAGCTAGATATCACATGCTGCCGAAAGATAACTTATGCTTCTATTGCCCAGTTGTCAGAATCATGTACCGCACTCACTTCTCTTAGGATGGAGTCATGTACCCTGGTTCCTCGAGAAGCATTTGTCCTTATTGGACAGAGATGCCAAACTCTTGAGGAGATTGACATAACAGATAATGAAGTCGACGACGAAGGTCTGAAGTCCATTTCTAGATGTTCCAACCTCTCCAGCTTAAAAGTAGGAATTTGCCTGAACATAACTAATGACGGCGTTGTGAACATTGGCATGCGCTGTTCAAAACTAGTAGAGCTCGATCTATACAGGTGCACAGGAATTTCAGATTCAGGCATATCAGCTATTGCTAGAGGTTGTCCTGGACTTGAGGTTATTAACATAGCCTATTGCAAAGATATTACTGATAGTTCGTTAATCTCGTTGTCAAAATGCTCAAGTCTGAACACTGTTGAAAGTCGAGGATGTCCTCTTATCACATCTCTGGGTCTAGCAGCAATTGCTGTGGGATGCAAGCAACTGACCAAGCTGGATGTAAAGAAGTGTTCAAATATTGATGATGCTGGAATGATCCTGCTTGCTCACTTTTCTCAGAACCTTAGACAAATTAATTTGTCATATACCTCAGTTACAGATGTGGGGCTATTATCCTTAGGCAGTATCAGTTGCCTACAGAGCTTAACCATCCTGCACTTGGAGGGCTTGAGCCCTAGTGGACTGGCAGCTGCCTTATTGGCCTGTGGGGGGCTAACCAAAGTAAAGCTCCAAGCAACCTTCAAGTCATTGCTACCCCAAGCTCTTTACGAACATTTAGAAGCACGCGGTTGTGTATTTCAGTGGAGAAATAAGTTCTTCAGGGCTGAATTGGACCCTCAGTGTTGGAAAATACAATTAGAAGATATCATGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_6

Protein Analysis

672

Amino Acids

73.12

Weight (kDa)

8.29

Isoelectric Point (pI)

38.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7885 PF25372 84 - 199 8.5e-08 Leucine Rich Repeat Domain of unknown function (DUF7885)
DUF7885 PF25372 206 - 286 2.2e-06 Leucine Rich Repeat Domain of unknown function (DUF7885)
DUF7885 PF25372 304 - 399 4.7e-07 Leucine Rich Repeat Domain of unknown function (DUF7885)
DUF7885 PF25372 335 - 484 1.1e-13 Leucine Rich Repeat Domain of unknown function (DUF7885)
DUF7885 PF25372 524 - 618 5.1e-06 Leucine Rich Repeat Domain of unknown function (DUF7885)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 50, 596, 937
Acc36I ACCTGC 1 cut(s) 300
AccB1I GGYRCC 1 cut(s) 573
AccI GTMKAC 1 cut(s) 1275
AccII CGCG 5 cut(s) 212, 268, 340, 443, 1926
AclWI GGATC 2 cut(s) 648, 1669
AcsI RAATTY 4 cut(s) 469, 1084, 1335, 1425
AcuI CTGAAG 3 cut(s) 712, 1310, 1942
AfaI GTAC 2 cut(s) 1159, 1192
AfiI CCNNNNNNNGG 2 cut(s) 1798, 1848
AflII CTTAAG 2 cut(s) 779, 1024
AgsI TTSAA 8 cut(s) 568, 704, 760, 1072, 1389, 1559, 1653, 1882
AhdI GACNNNNNGTC 1 cut(s) 124
AhlI ACTAGT 1 cut(s) 1393
AjiI CACGTC 1 cut(s) 247
AjnI CCWGG 3 cut(s) 960, 1194, 1465
Alw21I GWGCWC 3 cut(s) 432, 1404, 1421
Alw26I GTCTC 2 cut(s) 429, 1061
Alw44I GTGCAC 1 cut(s) 1417
AlwI GGATC 2 cut(s) 648, 1669
Ama87I CYCGRG 2 cut(s) 355, 1203
AoxI GGCC 3 cut(s) 745, 753, 1839
ApaLI GTGCAC 1 cut(s) 1417
ApeKI GCWGC 5 cut(s) 380, 1110, 1598, 1826, 1829
ApoI RAATTY 4 cut(s) 469, 1084, 1335, 1425
AseI ATTAAT 3 cut(s) 557, 561, 1713
Asp700I GAANNNNTTC 1 cut(s) 350
AspLEI GCGC 2 cut(s) 198, 1383
AspS9I GGNCC 3 cut(s) 15, 312, 1972
AsuC2I CCSGG 1 cut(s) 275
AsuHPI GGTGA 1 cut(s) 1054
AsuII TTCGAA 1 cut(s) 631
AvaI CYCGRG 2 cut(s) 355, 1203
AvaII GGWCC 3 cut(s) 15, 312, 1972
AxyI CCTNAGG 1 cut(s) 1756
BaeGI GKGCMC 1 cut(s) 1421
BanI GGYRCC 1 cut(s) 573
BanII GRGCYC 3 cut(s) 432, 1404, 1814
BbsI GAAGAC 1 cut(s) 46
Bbv12I GWGCWC 3 cut(s) 432, 1404, 1421
BbvI GCAGC 5 cut(s) 367, 1097, 1610, 1816, 1838
BccI CCATC 8 cut(s) 302, 481, 685, 737, 947, 995, 1174, 1796
BceAI ACGGC 1 cut(s) 1375
BciT130I CCWGG 3 cut(s) 962, 1196, 1467
BcnI CCSGG 1 cut(s) 275
BcoDI GTCTC 2 cut(s) 429, 1061
BcuI ACTAGT 1 cut(s) 1393
BfaI CTAG 8 cut(s) 716, 798, 1097, 1302, 1394, 1455, 1595, 1815
BfmI CTRYAG 2 cut(s) 307, 1775
BfrI CTTAAG 2 cut(s) 779, 1024
BfuAI ACCTGC 1 cut(s) 300
BglI GCCNNNNNGGC 1 cut(s) 1838
BglII AGATCT 1 cut(s) 625
BisI GCNGC 7 cut(s) 159, 381, 450, 1111, 1599, 1827, 1830
BlpI GCTNAGC 1 cut(s) 1019
BlsI GCNGC 7 cut(s) 160, 382, 451, 1112, 1600, 1828, 1831
Bme1390I CCNGG 4 cut(s) 275, 962, 1196, 1467
Bme18I GGWCC 3 cut(s) 15, 312, 1972
BmeRI GACNNNNNGTC 1 cut(s) 124
BmeT110I CYCGRG 2 cut(s) 355, 1203
BmgBI CACGTC 1 cut(s) 247
BmgT120I GGNCC 3 cut(s) 15, 312, 1972
BmiI GGNNCC 4 cut(s) 241, 575, 1200, 1974
BmrFI CCNGG 4 cut(s) 275, 962, 1196, 1467
BmrI ACTGGG 1 cut(s) 1135
BmsI GCATC 6 cut(s) 181, 659, 713, 1223, 1604, 1654
BmuI ACTGGG 1 cut(s) 1135
BoxI GACNNNNGTC 1 cut(s) 1284
BpiI GAAGAC 1 cut(s) 46
BplI GAGNNNNNCTC 2 cut(s) 1043, 1075
BpmI CTGGAG 1 cut(s) 1303
Bpu1102I GCTNAGC 1 cut(s) 1019
Bpu14I TTCGAA 1 cut(s) 631
BpuEI CTTGAG 5 cut(s) 170, 1265, 1493, 1525, 1828
BpuMI CCSGG 1 cut(s) 275
Bsa29I ATCGAT 4 cut(s) 326, 330, 680, 735
BsaI GGTCTC 1 cut(s) 1061
BsaJI CCNNGG 2 cut(s) 441, 1194
BsaXI ACNNNNNCTCC 2 cut(s) 467, 497
Bsc4I CCNNNNNNNGG 2 cut(s) 1798, 1848
Bse1I ACTGG 3 cut(s) 1001, 1141, 1827
Bse21I CCTNAGG 1 cut(s) 1756
Bse3DI GCAATG 1 cut(s) 1886
BseBI CCWGG 3 cut(s) 962, 1196, 1467
BseCI ATCGAT 4 cut(s) 326, 330, 680, 735
BseDI CCNNGG 2 cut(s) 441, 1194
BseGI GGATG 8 cut(s) 492, 728, 860, 1185, 1574, 1619, 1642, 1788
BseLI CCNNNNNNNGG 2 cut(s) 1798, 1848
BseMI GCAATG 1 cut(s) 1886
BseMII CTCAG 7 cut(s) 385, 445, 542, 1010, 1709, 1743, 1991
BseNI ACTGG 3 cut(s) 1001, 1141, 1827
BseRI GAGGAG 2 cut(s) 1051, 1262
BseSI GKGCMC 1 cut(s) 1421
BseXI GCAGC 5 cut(s) 367, 1097, 1610, 1816, 1838
BsgI GTGCAG 1 cut(s) 1778
Bsh1236I CGCG 5 cut(s) 212, 268, 340, 443, 1926
Bsh1285I CGRYCG 3 cut(s) 326, 330, 344
BshFI GGCC 3 cut(s) 747, 755, 1841
BshNI GGYRCC 1 cut(s) 573
BshVI ATCGAT 4 cut(s) 326, 330, 680, 735
BsiEI CGRYCG 3 cut(s) 326, 330, 344
BsiHKAI GWGCWC 3 cut(s) 432, 1404, 1421
BsiHKCI CYCGRG 2 cut(s) 355, 1203
BsiSI CCGG 1 cut(s) 275
BslFI GGGAC 1 cut(s) 257
BslI CCNNNNNNNGG 2 cut(s) 1798, 1848
BsmAI GTCTC 2 cut(s) 429, 1061
BsmFI GGGAC 1 cut(s) 257
BsnI GGCC 3 cut(s) 747, 755, 1841
Bso31I GGTCTC 1 cut(s) 1061
BsoBI CYCGRG 2 cut(s) 355, 1203
Bsp119I TTCGAA 1 cut(s) 631
Bsp1286I GDGCHC 4 cut(s) 432, 1404, 1421, 1814
Bsp1720I GCTNAGC 1 cut(s) 1019
Bsp68I TCGCGA 2 cut(s) 212, 268
BspANI GGCC 3 cut(s) 747, 755, 1841
BspCNI CTCAG 7 cut(s) 384, 444, 543, 1011, 1708, 1742, 1990
BspDI ATCGAT 4 cut(s) 326, 330, 680, 735
BspFNI CGCG 5 cut(s) 212, 268, 340, 443, 1926
BspLI GGNNCC 4 cut(s) 241, 575, 1200, 1974
BspMAI CTGCAG 1 cut(s) 311
BspMI ACCTGC 1 cut(s) 300
BspPI GGATC 2 cut(s) 648, 1669
BspT104I TTCGAA 1 cut(s) 631
BspT107I GGYRCC 1 cut(s) 573
BspTI CTTAAG 2 cut(s) 779, 1024
BspTNI GGTCTC 1 cut(s) 1061
BsrDI GCAATG 1 cut(s) 1886
BsrI ACTGG 3 cut(s) 1001, 1141, 1827
BssECI CCNNGG 2 cut(s) 441, 1194
Bst2UI CCWGG 3 cut(s) 962, 1196, 1467
Bst4CI ACNGT 1 cut(s) 1555
BstAFI CTTAAG 2 cut(s) 779, 1024
BstAPI GCANNNNNTGC 1 cut(s) 1604
BstBI TTCGAA 1 cut(s) 631
BstC8I GCNNGC 8 cut(s) 266, 307, 609, 674, 1379, 1619, 1683, 1924
BstDSI CCRYGG 1 cut(s) 441
BstEII GGTNACC 1 cut(s) 964
BstF5I GGATG 8 cut(s) 492, 728, 860, 1185, 1574, 1619, 1642, 1788
BstFNI CGCG 5 cut(s) 212, 268, 340, 443, 1926
BstHHI GCGC 2 cut(s) 198, 1383
BstMAI GTCTC 2 cut(s) 429, 1061
BstMCI CGRYCG 3 cut(s) 326, 330, 344
BstMWI GCNNNNNNNGC 7 cut(s) 265, 449, 455, 1136, 1604, 1779, 1838
BstNI CCWGG 3 cut(s) 962, 1196, 1467
BstNSI RCATGY 2 cut(s) 1110, 1381
BstPAI GACNNNNGTC 1 cut(s) 1284
BstPI GGTNACC 1 cut(s) 964
BstSCI CCNGG 4 cut(s) 273, 960, 1194, 1465
BstSFI CTRYAG 2 cut(s) 307, 1775
BstSLI GKGCMC 1 cut(s) 1421
BstUI CGCG 5 cut(s) 212, 268, 340, 443, 1926
BstV1I GCAGC 5 cut(s) 367, 1097, 1610, 1816, 1838
BstV2I GAAGAC 1 cut(s) 46
BstX2I RGATCY 2 cut(s) 625, 640
BstYI RGATCY 2 cut(s) 625, 640
Bsu15I ATCGAT 4 cut(s) 326, 330, 680, 735
Bsu36I CCTNAGG 1 cut(s) 1756
BsuRI GGCC 3 cut(s) 747, 755, 1841
BsuTUI ATCGAT 4 cut(s) 326, 330, 680, 735
BtgI CCRYGG 1 cut(s) 441
BtgZI GCGATG 1 cut(s) 158
BtrI CACGTC 1 cut(s) 247
BtsCI GGATG 8 cut(s) 492, 728, 860, 1185, 1574, 1619, 1642, 1788
BtsIMutI CAGTG 3 cut(s) 1551, 1946, 1985
BtuMI TCGCGA 2 cut(s) 212, 268
BveI ACCTGC 1 cut(s) 300
Cac8I GCNNGC 8 cut(s) 266, 307, 609, 674, 1379, 1619, 1683, 1924
CfoI GCGC 2 cut(s) 198, 1383
Cfr13I GGNCC 3 cut(s) 15, 312, 1972
Cfr42I CCGCGG 1 cut(s) 444
ClaI ATCGAT 4 cut(s) 326, 330, 680, 735
CseI GACGC 1 cut(s) 287
Csp6I GTAC 2 cut(s) 1158, 1191
CspCI CAANNNNNGTGG 2 cut(s) 1590, 1625
CviAII CATG 8 cut(s) 59, 764, 818, 1107, 1155, 1188, 1378, 2008
CviQI GTAC 2 cut(s) 1158, 1191
DrdI GACNNNNNNGTC 3 cut(s) 50, 596, 937
DriI GACNNNNNGTC 1 cut(s) 124
DseDI GACNNNNNNGTC 3 cut(s) 50, 596, 937
Eam1105I GACNNNNNGTC 1 cut(s) 124
EciI GGCGGA 1 cut(s) 473
Ecl136II GAGCTC 2 cut(s) 430, 1402
Eco24I GRGCYC 3 cut(s) 432, 1404, 1814
Eco31I GGTCTC 1 cut(s) 1061
Eco32I GATATC 2 cut(s) 1102, 2005
Eco47I GGWCC 3 cut(s) 15, 312, 1972
Eco53kI GAGCTC 2 cut(s) 430, 1402
Eco57I CTGAAG 3 cut(s) 712, 1310, 1942
Eco81I CCTNAGG 1 cut(s) 1756
Eco88I CYCGRG 2 cut(s) 355, 1203
Eco91I GGTNACC 1 cut(s) 964
EcoICRI GAGCTC 2 cut(s) 430, 1402
EcoO65I GGTNACC 1 cut(s) 964
EcoRII CCWGG 3 cut(s) 960, 1194, 1465
EcoRV GATATC 2 cut(s) 1102, 2005
EcoT38I GRGCYC 3 cut(s) 432, 1404, 1814
FaeI CATG 8 cut(s) 62, 767, 821, 1110, 1158, 1191, 1381, 2011
FaqI GGGAC 1 cut(s) 257
FatI CATG 8 cut(s) 58, 763, 817, 1106, 1154, 1187, 1377, 2007
FauNDI CATATG 1 cut(s) 880
FblI GTMKAC 1 cut(s) 1275
Fnu4HI GCNGC 7 cut(s) 159, 381, 450, 1111, 1599, 1827, 1830
FokI GGATG 8 cut(s) 499, 735, 867, 1192, 1581, 1626, 1649, 1775
FriOI GRGCYC 3 cut(s) 432, 1404, 1814
Fsp4HI GCNGC 7 cut(s) 159, 381, 450, 1111, 1599, 1827, 1830
FspBI CTAG 8 cut(s) 716, 798, 1097, 1302, 1394, 1455, 1595, 1815
GlaI GCGC 2 cut(s) 197, 1382
GluI GCNGC 7 cut(s) 159, 381, 450, 1111, 1599, 1827, 1830
GsuI CTGGAG 1 cut(s) 1303
HaeIII GGCC 3 cut(s) 747, 755, 1841
HapII CCGG 1 cut(s) 275
HgaI GACGC 1 cut(s) 287
HhaI GCGC 2 cut(s) 198, 1383
Hin1II CATG 8 cut(s) 62, 767, 821, 1110, 1158, 1191, 1381, 2011
Hin6I GCGC 2 cut(s) 196, 1381
HinP1I GCGC 2 cut(s) 196, 1381
HincII GTYRAC 1 cut(s) 1276
HindII GTYRAC 1 cut(s) 1276
HindIII AAGCTT 1 cut(s) 923
HinfI GANTC 9 cut(s) 164, 216, 270, 394, 437, 895, 1151, 1184, 1433
HpaII CCGG 1 cut(s) 275
HphI GGTGA 1 cut(s) 1054
Hpy166II GTNNAC 5 cut(s) 315, 1276, 1369, 1419, 1820
Hpy188III TCNNGA 8 cut(s) 211, 267, 357, 701, 716, 1205, 1244, 1302
Hpy8I GTNNAC 5 cut(s) 315, 1276, 1369, 1419, 1820
Hpy99I CGWCG 5 cut(s) 251, 293, 934, 1280, 1283
HpyAV CCTTC 6 cut(s) 139, 160, 713, 766, 1277, 1888
HpyCH4III ACNGT 1 cut(s) 1555
HpyCH4IV ACGT 3 cut(s) 51, 246, 932
HpyF10VI GCNNNNNNNGC 7 cut(s) 265, 449, 455, 1136, 1604, 1779, 1838
HpySE526I ACGT 3 cut(s) 51, 246, 932
Hsp92II CATG 8 cut(s) 62, 767, 821, 1110, 1158, 1191, 1381, 2011
HspAI GCGC 2 cut(s) 196, 1381
KspI CCGCGG 1 cut(s) 444
LmnI GCTCC 4 cut(s) 203, 245, 1015, 1872
Lsp1109I GCAGC 5 cut(s) 367, 1097, 1610, 1816, 1838
LweI GCATC 6 cut(s) 181, 659, 713, 1223, 1604, 1654
MaeI CTAG 8 cut(s) 716, 798, 1097, 1302, 1394, 1455, 1595, 1815
MaeII ACGT 3 cut(s) 51, 246, 932
MaeIII GTNAC 7 cut(s) 690, 774, 848, 892, 964, 1042, 1732
MboII GAAGA 8 cut(s) 16, 46, 478, 620, 676, 716, 1948, 2012
MfeI CAATTG 2 cut(s) 114, 1602
MflI RGATCY 2 cut(s) 625, 640
MhlI GDGCHC 4 cut(s) 432, 1404, 1421, 1814
MlyI GAGTC 5 cut(s) 225, 279, 431, 889, 1193
MmeI TCCRAC 5 cut(s) 225, 580, 953, 1335, 1964
MroXI GAANNNNTTC 1 cut(s) 350
MseI TTAA 9 cut(s) 557, 561, 780, 1025, 1325, 1482, 1520, 1713, 1784
MslI CAYNNNNRTG 1 cut(s) 1376
MspA1I CMGCKG 2 cut(s) 443, 1829
MspCI CTTAAG 2 cut(s) 779, 1024
MspI CCGG 1 cut(s) 275
MspR9I CCNGG 4 cut(s) 275, 962, 1196, 1467
MunI CAATTG 2 cut(s) 114, 1602
MvaI CCWGG 3 cut(s) 962, 1196, 1467
MvnI CGCG 5 cut(s) 212, 268, 340, 443, 1926
MwoI GCNNNNNNNGC 7 cut(s) 265, 449, 455, 1136, 1604, 1779, 1838
NciI CCSGG 1 cut(s) 275
NdeI CATATG 1 cut(s) 880
NlaIII CATG 8 cut(s) 62, 767, 821, 1110, 1158, 1191, 1381, 2011
NlaIV GGNNCC 4 cut(s) 241, 575, 1200, 1974
NmuCI GTSAC 4 cut(s) 774, 848, 892, 1042
NruI TCGCGA 2 cut(s) 212, 268
NspI RCATGY 2 cut(s) 1110, 1381
NspV TTCGAA 1 cut(s) 631
PacI TTAATTAA 1 cut(s) 561
PaeI GCATGC 1 cut(s) 1381
PaeR7I CTCGAG 1 cut(s) 1203
PcsI WCGNNNNNNNCGW 4 cut(s) 48, 99, 285, 1523
PdmI GAANNNNTTC 1 cut(s) 350
PfeI GAWTC 4 cut(s) 164, 394, 1151, 1433
PfoI TCCNGGA 2 cut(s) 273, 1465
PkrI GCNGC 7 cut(s) 160, 382, 451, 1112, 1600, 1828, 1831
Ple19I CGATCG 2 cut(s) 326, 330
PleI GAGTC 5 cut(s) 224, 278, 431, 889, 1192
PpsI GAGTC 5 cut(s) 224, 278, 431, 889, 1192
PshAI GACNNNNGTC 1 cut(s) 1284
PshBI ATTAAT 3 cut(s) 557, 561, 1713
Psp124BI GAGCTC 2 cut(s) 432, 1404
Psp6I CCWGG 3 cut(s) 960, 1194, 1465
PspEI GGTNACC 1 cut(s) 964
PspGI CCWGG 3 cut(s) 960, 1194, 1465
PspN4I GGNNCC 4 cut(s) 241, 575, 1200, 1974
PspPI GGNCC 3 cut(s) 15, 312, 1972
PstI CTGCAG 1 cut(s) 311
PsuI RGATCY 2 cut(s) 625, 640
PvuI CGATCG 2 cut(s) 326, 330
PvuII CAGCTG 1 cut(s) 1829
RruI TCGCGA 2 cut(s) 212, 268
RsaI GTAC 2 cut(s) 1159, 1192
RsaNI GTAC 2 cut(s) 1158, 1191
RseI CAYNNNNRTG 1 cut(s) 1376
SacI GAGCTC 2 cut(s) 432, 1404
SacII CCGCGG 1 cut(s) 444
SalI GTCGAC 1 cut(s) 1274
SaqAI TTAA 9 cut(s) 557, 561, 780, 1025, 1325, 1482, 1520, 1713, 1784
SatI GCNGC 7 cut(s) 159, 381, 450, 1111, 1599, 1827, 1830
Sau96I GGNCC 3 cut(s) 15, 312, 1972
SbfI CCTGCAGG 1 cut(s) 311
SchI GAGTC 5 cut(s) 225, 279, 431, 889, 1193
ScrFI CCNGG 4 cut(s) 275, 962, 1196, 1467
SdaI CCTGCAGG 1 cut(s) 311
SduI GDGCHC 4 cut(s) 432, 1404, 1421, 1814
SfaNI GCATC 6 cut(s) 181, 659, 713, 1223, 1604, 1654
SfcI CTRYAG 2 cut(s) 307, 1775
Sfr274I CTCGAG 1 cut(s) 1203
Sfr303I CCGCGG 1 cut(s) 444
SfuI TTCGAA 1 cut(s) 631
SgrBI CCGCGG 1 cut(s) 444
SinI GGWCC 3 cut(s) 15, 312, 1972
SlaI CTCGAG 1 cut(s) 1203
SmiMI CAYNNNNRTG 1 cut(s) 1376
SmlI CTYRAG 8 cut(s) 185, 779, 1024, 1203, 1244, 1472, 1540, 1807
SmoI CTYRAG 8 cut(s) 185, 779, 1024, 1203, 1244, 1472, 1540, 1807
SpeI ACTAGT 1 cut(s) 1393
SphI GCATGC 1 cut(s) 1381
Sse8387I CCTGCAGG 1 cut(s) 311
SspI AATATT 1 cut(s) 1657
SspMI CTAG 8 cut(s) 716, 798, 1097, 1302, 1394, 1455, 1595, 1815
SstI GAGCTC 2 cut(s) 432, 1404
StyD4I CCNGG 4 cut(s) 273, 960, 1194, 1465
TaaI ACNGT 1 cut(s) 1555
TaiI ACGT 3 cut(s) 54, 249, 935
TauI GCSGC 2 cut(s) 161, 452
TfiI GAWTC 4 cut(s) 164, 394, 1151, 1433
Tru1I TTAA 9 cut(s) 557, 561, 780, 1025, 1325, 1482, 1520, 1713, 1784
Tru9I TTAA 9 cut(s) 557, 561, 780, 1025, 1325, 1482, 1520, 1713, 1784
TscAI CASTG 3 cut(s) 1558, 1946, 1985
TseFI GTSAC 4 cut(s) 774, 848, 892, 1042
TseI GCWGC 5 cut(s) 380, 1110, 1598, 1826, 1829
Tsp45I GTSAC 4 cut(s) 774, 848, 892, 1042
TspDTI ATGAA 4 cut(s) 17, 73, 87, 1284
TspGWI ACGGA 3 cut(s) 524, 569, 867
TspRI CASTG 3 cut(s) 1558, 1946, 1985
Vha464I CTTAAG 2 cut(s) 779, 1024
VneI GTGCAC 1 cut(s) 1417
VpaK11BI GGWCC 3 cut(s) 15, 312, 1972
VspI ATTAAT 3 cut(s) 557, 561, 1713
XapI RAATTY 4 cut(s) 469, 1084, 1335, 1425
XbaI TCTAGA 2 cut(s) 715, 1301
XceI RCATGY 2 cut(s) 1110, 1381
XcmI CCANNNNNNNNNTGG 1 cut(s) 1795
XhoI CTCGAG 1 cut(s) 1203
XmiI GTMKAC 1 cut(s) 1275
XmnI GAANNNNTTC 1 cut(s) 350
XspI CTAG 8 cut(s) 716, 798, 1097, 1302, 1394, 1455, 1595, 1815
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.