Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0007838

Physical Location & Seq

Genomic Coordinates Forward (+)

26359050 .. 26363809

Gene Structure 4760 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1183500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1962 bp

ATGATAGCTGCGACGTCGTCGTTATCGTCGTCTTCGGCGAGTGTGGCTGTGGACAAGGCGACGAGCGATCTTCTGATAAGTCCTGATTGGACTATGAACATAGACATCTGCGATTCCGTCAATTCTCATCATTGGCAGGCAAAAGATGTCGTAAAAGCTGTTAAGAAAAGGTTGCAGCATAAGAGCCCCAAAGTTCAATTTCTAGCCTTGACGCTTTTGGAGACAATGGTGAAGAACTGTGGTGATTATGTCCATGTTCAAATTGCTGAGAGAAATATACTAGGAGAGATGGTTAAAATTGTGAGAAAGAGGGCAGATATGCAGGTTCGGGATAAAATCTTGATATTGCTGGACTCTTGGCAAGAGGCTTTCGGTGGTCCTGCTGGAAAACATCCTCAGTACTACTGGGCATACGAGGAACTAAGGCGTTCCGGAGTACAATTTCCCAGGCGTGCATCAGAAGCAGCTCCAATATTTACACCACCTGTTGCAAATACAGCATTGAGGCACACTCAAGCTGGATACGGAATGCCTAGCAATTCCTCAAGAAGGCTCGATGAAACAATGGCTACAGAAATGGAGAATCTAAGTTTGTCAAACCTGGATTCTATGAGGAATGTTATGGAGCTCTTAGATGACATGCTGCAAGCTGTGAACCCTAGTGACCGTTTGGCCATAAAAGATGAAGTGATAGAGGAGCTTGTCAATGGATGTCGTGCCAACCAGAAAAAACTGATCCATATGCTAACTACAACTGGAGATGAAGAACTTCTTGCTCGGGGTCTTGATTTGAACGATAGGATGCAAAGTCTGCTTGCAAAACATGATGCTATAGTTTCTGGTTTGCCTGTGCCAACTCAAGCTACAAGCTTTAGTCCCCAACCGACTGAAAAGTTTGCGTCAAGTTCCAAGCAGACTGAAGTAAAAGATTCCAGCACAAGTGACTCTAGTCCAAGACCTAATGCTAATGTATCTGCACCAGTTGGTTCCATGGCCAGGATCCAGATTGATGAAGACGAAGAAGAGGAAGATGAATTTGCACAGCTAGCTCGAAGGCATTTTAAACCACGGTCGGTGCCTCCTCAAAACACATCTAGTGGAAGTAATGATAATTTGGCGTTGGTGAGCACCAGCAGCACAATAGATCCATCAGCCACAACATCCGCTCCAAGCAATGCATTAGTTCTGGCTGATCCACCAGCACCGGTTAAGACAACAAAAGAGCAGGACATGATCGACCTTCTGAGTATCACTTTATCAACAACAGCTGCCTCCCCAGACAACACCCCTCATGCTCTAGCATCCAACCCTCACACCACACCAGACCCGCACACGGTTCCAGCATCTAATGGTAACACACATCAGGAGCCTGTTGCCCCCACCATACAAGGAAATCCCAATCCTTCCCAGCCTTATCCTGGAAATCCGGGGACGGTATCCTATAACAGCTATGTTGTTCCATGGGCGCAAGCACAACCTCAATCCCACCTCCAACAGCCACAGTTCCAACCGCAATCACAATTTCAGCCTCAGCCCCAATTCCAGTCACAATTTCAACCTCAATATCCTCAATACCCGTCTGCCTACCCTCCTCCACCGTGGGCAGCTTCACCGGGATATTCTAGCCAAAACCATTTTTCCGCCAATAATACATTTTCAACTCCTCGTGCCAACACAACCACATCTTATAGTCCAGCACAATCAGCTAGACCTTTGCACCACTTCAATTCGTTCCCTGCAAGGGGAATGAATGGGATAGATAGCAACGGAGGTTCAACGAGCCCCGGTCCTAGGAGCCCTGCCCCTTCAGCAGGACAAAAGCCCTACATTCCCACGTACCGATTATTTGAAGATCTGAATGTGTTTGGCAATGCAGATGGAAAGTTTAAGATGACAAGCAGCAATACATCATCTAGCTTGTCTGGAGCTGCCGGCCAAGGTATGGTAGGTGGGAGGAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005886 GO:0006508 GO:0006511 GO:0006807 GO:0006810 GO:0006886 GO:0007034 GO:0007088 GO:0007154 GO:0007165 GO:0007346 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010564 GO:0010639 GO:0010948 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016192 GO:0016197 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0019941 GO:0023052 GO:0030163 GO:0030276 GO:0031410 GO:0031982 GO:0032182 GO:0032509 GO:0032511 GO:0033036 GO:0033043 GO:0033365 GO:0034613 GO:0042886 GO:0043130 GO:0043162 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043328 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045184 GO:0045324 GO:0045786 GO:0045839 GO:0045930 GO:0046907 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051129 GO:0051179 GO:0051234 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0051726 GO:0051783 GO:0051784 GO:0065007 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0071944 GO:0071985 GO:0072594 GO:0072665 GO:0072666 GO:0097708 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GAT VHS

Protein Analysis

654

Amino Acids

70.85

Weight (kDa)

6.11

Isoelectric Point (pI)

54.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
VHS	PF00790	11 - 124	3.4e-32	VHS domain
GAT	PF03127	205 - 281	1.9e-17	GAT domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007838)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G38410
fragaria_vesca	FvH4_6g10390 FvH4_6g10390 FvH4_6g10390 FvH4_6g10390 FvH4_6g10390
malus_domestica	MD04G1169500.v1.1 MD12G1183500.v1.1
prunus_persica	Prupe.6G288000_v2.0.a1 Prupe.6G288000_v2.0.a1
pyrus_communis	pycom04g15020 pycom04g15030 pycom12g17210
rosa_chinensis	RchiOBHm_Chr3g0461281
rosa_laevigata	RLG00000024944
rosa_multiflora	Rmu_ssc0000326.1_g000008
rosa_roxburghii	Rroxscaffold_6G00418440 Rroxscaffold_6G00418730
rosa_rugosa	Rorug03G0049400
rosa_samantha	Rh3AG106700 Rh3BG110900 Rh3CG112800 Rh3DG112900
rosa_wichuraiana	Rw3G009140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	17
Acc36I	ACCTGC	1 cut(s)	313
AccB1I	GGYRCC	1 cut(s)	1075
AccB7I	CCANNNNNTGG	1 cut(s)	1942
AccBSI	CCGCTC	1 cut(s)	1166
AccIII	TCCGGA	1 cut(s)	431
AciI	CCGC	4 cut(s)	1164, 1328, 1511, 1641
AclWI	GGATC	5 cut(s)	730, 994, 1007, 1139, 1187
AcoI	YGGCCR	3 cut(s)	672, 993, 1933
AcsI	RAATTY	1 cut(s)	1034
AcuI	CTGAAG	2 cut(s)	939, 1791
AcyI	GRCGYC	1 cut(s)	14
AfaI	GTAC	3 cut(s)	401, 438, 1838
AfiI	CCNNNNNNNGG	9 cut(s)	549, 996, 1333, 1418, 1741, 1742, 1791, 1811, 1942
AgeI	ACCGGT	1 cut(s)	1204
AgsI	TTSAA	8 cut(s)	197, 260, 793, 1556, 1659, 1726, 1776, 1850
AjnI	CCWGG	4 cut(s)	446, 600, 995, 1417
Alw21I	GWGCWC	2 cut(s)	630, 1130
Alw26I	GTCTC	1 cut(s)	215
AlwI	GGATC	5 cut(s)	730, 994, 1007, 1139, 1187
Ama87I	CYCGRG	1 cut(s)	777
Aor13HI	TCCGGA	1 cut(s)	431
AoxI	GGCC	3 cut(s)	672, 993, 1933
ApeKI	GCWGC	9 cut(s)	8, 175, 464, 643, 1134, 1268, 1604, 1899, 1928
ApoI	RAATTY	1 cut(s)	1034
AsiGI	ACCGGT	1 cut(s)	1204
Asp700I	GAANNNNTTC	1 cut(s)	768
AspA2I	CCTAGG	1 cut(s)	1790
AspLEI	GCGC	1 cut(s)	1468
AspS9I	GGNCC	2 cut(s)	377, 1787
AsuC2I	CCSGG	3 cut(s)	1428, 1614, 1785
AsuHPI	GGTGA	4 cut(s)	241, 254, 1135, 1602
AsuNHI	GCTAGC	1 cut(s)	1045
AvaI	CYCGRG	1 cut(s)	777
AvaII	GGWCC	2 cut(s)	377, 1787
AvrII	CCTAGG	1 cut(s)	1790
BalI	TGGCCA	2 cut(s)	674, 995
BamHI	GGATCC	1 cut(s)	999
BanI	GGYRCC	1 cut(s)	1075
BanII	GRGCYC	4 cut(s)	188, 630, 1784, 1799
BauI	CACGAG	1 cut(s)	1665
BbsI	GAAGAC	2 cut(s)	24, 1020
Bbv12I	GWGCWC	2 cut(s)	630, 1130
BbvCI	CCTCAGC	1 cut(s)	1530
BbvI	GCAGC	8 cut(s)	187, 476, 630, 1146, 1255, 1616, 1911, 1915
BccI	CCATC	3 cut(s)	283, 1156, 1871
BciT130I	CCWGG	4 cut(s)	448, 602, 997, 1419
BciVI	GTATCC	2 cut(s)	515, 1447
BcnI	CCSGG	3 cut(s)	1428, 1614, 1785
BcoDI	GTCTC	1 cut(s)	215
BfmI	CTRYAG	2 cut(s)	570, 831
BfuAI	ACCTGC	1 cut(s)	313
BfuI	GTATCC	2 cut(s)	515, 1447
BglII	AGATCT	1 cut(s)	1852
BisI	GCNGC	9 cut(s)	9, 176, 465, 644, 1135, 1269, 1605, 1900, 1929
BlnI	CCTAGG	1 cut(s)	1790
BlsI	GCNGC	9 cut(s)	10, 177, 466, 645, 1136, 1270, 1606, 1901, 1930
BmcAI	AGTACT	1 cut(s)	401
Bme1390I	CCNGG	7 cut(s)	448, 602, 997, 1419, 1428, 1614, 1785
Bme18I	GGWCC	2 cut(s)	377, 1787
BmeT110I	CYCGRG	1 cut(s)	777
BmgT120I	GGNCC	2 cut(s)	377, 1787
BmiI	GGNNCC	6 cut(s)	988, 1001, 1077, 1338, 1368, 1796
BmrFI	CCNGG	7 cut(s)	448, 602, 997, 1419, 1428, 1614, 1785
BmrI	ACTGGG	1 cut(s)	415
BmsI	GCATC	5 cut(s)	464, 792, 817, 1310, 1352
BmtI	GCTAGC	1 cut(s)	1049
BmuI	ACTGGG	1 cut(s)	415
BoxI	GACNNNNGTC	1 cut(s)	948
BpiI	GAAGAC	2 cut(s)	24, 1020
BplI	GAGNNNNNCTC	2 cut(s)	496, 528
BpmI	CTGGAG	2 cut(s)	777, 1944
Bpu10I	CCTNAGC	1 cut(s)	1530
BpuEI	CTTGAG	3 cut(s)	498, 529, 843
BpuMI	CCSGG	3 cut(s)	1428, 1614, 1785
BsaAI	YACGTR	1 cut(s)	1836
BsaHI	GRCGYC	1 cut(s)	14
BsaJI	CCNNGG	9 cut(s)	446, 990, 1067, 1427, 1460, 1598, 1783, 1790, 1936
BsaWI	WCCGGW	2 cut(s)	431, 1204
BsaXI	ACNNNNNCTCC	4 cut(s)	276, 306, 1150, 1180
Bsc4I	CCNNNNNNNGG	9 cut(s)	549, 996, 1333, 1418, 1741, 1742, 1791, 1811, 1942
Bse118I	RCCGGY	2 cut(s)	1204, 1931
Bse1I	ACTGG	4 cut(s)	410, 760, 980, 1543
Bse3DI	GCAATG	2 cut(s)	1180, 1876
BseAI	TCCGGA	1 cut(s)	431
BseBI	CCWGG	4 cut(s)	448, 602, 997, 1419
BseDI	CCNNGG	9 cut(s)	446, 990, 1067, 1427, 1460, 1598, 1783, 1790, 1936
BseGI	GGATG	5 cut(s)	391, 716, 807, 1160, 1301
BseLI	CCNNNNNNNGG	9 cut(s)	549, 996, 1333, 1418, 1741, 1742, 1791, 1811, 1942
BseMI	GCAATG	2 cut(s)	1180, 1876
BseMII	CTCAG	4 cut(s)	258, 410, 1235, 1544
BseNI	ACTGG	4 cut(s)	410, 760, 980, 1543
BseRI	GAGGAG	4 cut(s)	710, 1071, 1581, 1653
BseXI	GCAGC	8 cut(s)	187, 476, 630, 1146, 1255, 1616, 1911, 1915
BseYI	CCCAGC	1 cut(s)	1407
BsgI	GTGCAG	1 cut(s)	960
Bsh1285I	CGRYCG	1 cut(s)	1073
BshFI	GGCC	3 cut(s)	674, 995, 1935
BshNI	GGYRCC	1 cut(s)	1075
BshTI	ACCGGT	1 cut(s)	1204
BsiEI	CGRYCG	1 cut(s)	1073
BsiHKAI	GWGCWC	2 cut(s)	630, 1130
BsiHKCI	CYCGRG	1 cut(s)	777
BsiSI	CCGG	6 cut(s)	432, 1205, 1427, 1613, 1785, 1932
BslFI	GGGAC	2 cut(s)	861, 1444
BslI	CCNNNNNNNGG	9 cut(s)	549, 996, 1333, 1418, 1741, 1742, 1791, 1811, 1942
BsmAI	GTCTC	1 cut(s)	215
BsmFI	GGGAC	2 cut(s)	861, 1444
BsmI	GAATGC	1 cut(s)	534
BsnI	GGCC	3 cut(s)	674, 995, 1935
BsoBI	CYCGRG	1 cut(s)	777
Bsp1286I	GDGCHC	5 cut(s)	188, 630, 1130, 1784, 1799
Bsp13I	TCCGGA	1 cut(s)	431
Bsp143I	GATC	7 cut(s)	67, 735, 999, 1144, 1192, 1233, 1852
Bsp19I	CCATGG	2 cut(s)	990, 1460
BspACI	CCGC	4 cut(s)	1164, 1328, 1511, 1641
BspANI	GGCC	3 cut(s)	674, 995, 1935
BspCNI	CTCAG	4 cut(s)	259, 409, 1236, 1543
BspEI	TCCGGA	1 cut(s)	431
BspLI	GGNNCC	6 cut(s)	988, 1001, 1077, 1338, 1368, 1796
BspMI	ACCTGC	1 cut(s)	313
BspOI	GCTAGC	1 cut(s)	1049
BspPI	GGATC	5 cut(s)	730, 994, 1007, 1139, 1187
BspT107I	GGYRCC	1 cut(s)	1075
BsrBI	CCGCTC	1 cut(s)	1166
BsrDI	GCAATG	2 cut(s)	1180, 1876
BsrFI	RCCGGY	2 cut(s)	1204, 1931
BsrI	ACTGG	4 cut(s)	410, 760, 980, 1543
BssAI	RCCGGY	2 cut(s)	1204, 1931
BssECI	CCNNGG	9 cut(s)	446, 990, 1067, 1427, 1460, 1598, 1783, 1790, 1936
BssMI	GATC	7 cut(s)	67, 735, 999, 1144, 1192, 1233, 1852
BssNI	GRCGYC	1 cut(s)	14
BssSI	CACGAG	1 cut(s)	1665
BssT1I	CCWWGG	4 cut(s)	990, 1460, 1790, 1936
Bst2BI	CACGAG	1 cut(s)	1665
Bst2UI	CCWGG	4 cut(s)	448, 602, 997, 1419
Bst4CI	ACNGT	7 cut(s)	239, 668, 1071, 1336, 1435, 1503, 1599
Bst6I	CTCTTC	1 cut(s)	1017
BstACI	GRCGYC	1 cut(s)	14
BstAPI	GCANNNNNTGC	1 cut(s)	811
BstBAI	YACGTR	1 cut(s)	1836
BstC8I	GCNNGC	7 cut(s)	138, 453, 648, 816, 1047, 1470, 1933
BstDEI	CTNAG	7 cut(s)	267, 396, 422, 587, 631, 1244, 1530
BstDSI	CCRYGG	4 cut(s)	990, 1067, 1460, 1598
BstENI	CCTNNNNNAGG	2 cut(s)	547, 1809
BstF5I	GGATG	5 cut(s)	391, 716, 807, 1160, 1301
BstHHI	GCGC	1 cut(s)	1468
BstKTI	GATC	7 cut(s)	70, 738, 1002, 1147, 1195, 1236, 1855
BstMAI	GTCTC	1 cut(s)	215
BstMBI	GATC	7 cut(s)	67, 735, 999, 1144, 1192, 1233, 1852
BstMCI	CGRYCG	1 cut(s)	1073
BstMWI	GCNNNNNNNGC	8 cut(s)	44, 461, 497, 811, 1046, 1134, 1703, 1808
BstNI	CCWGG	4 cut(s)	448, 602, 997, 1419
BstNSI	RCATGY	1 cut(s)	643
BstPAI	GACNNNNGTC	1 cut(s)	948
BstSCI	CCNGG	7 cut(s)	446, 600, 995, 1417, 1426, 1612, 1783
BstSFI	CTRYAG	2 cut(s)	570, 831
BstV1I	GCAGC	8 cut(s)	187, 476, 630, 1146, 1255, 1616, 1911, 1915
BstV2I	GAAGAC	2 cut(s)	24, 1020
BstX2I	RGATCY	3 cut(s)	999, 1144, 1852
BstYI	RGATCY	3 cut(s)	999, 1144, 1852
BsuI	GTATCC	2 cut(s)	515, 1447
BsuRI	GGCC	3 cut(s)	674, 995, 1935
BtgI	CCRYGG	4 cut(s)	990, 1067, 1460, 1598
BtsCI	GGATG	5 cut(s)	391, 716, 807, 1160, 1301
BveI	ACCTGC	1 cut(s)	313
Cac8I	GCNNGC	7 cut(s)	138, 453, 648, 816, 1047, 1470, 1933
CfoI	GCGC	1 cut(s)	1468
Cfr10I	RCCGGY	2 cut(s)	1204, 1931
Cfr13I	GGNCC	2 cut(s)	377, 1787
CseI	GACGC	2 cut(s)	220, 888
Csp6I	GTAC	3 cut(s)	400, 437, 1837
CspAI	ACCGGT	1 cut(s)	1204
CviAII	CATG	7 cut(s)	254, 640, 824, 991, 1231, 1292, 1461
CviQI	GTAC	3 cut(s)	400, 437, 1837
DdeI	CTNAG	7 cut(s)	267, 396, 422, 587, 631, 1244, 1530
DpnI	GATC	7 cut(s)	69, 737, 1001, 1146, 1194, 1235, 1854
DpnII	GATC	7 cut(s)	67, 735, 999, 1144, 1192, 1233, 1852
DraI	TTTAAA	1 cut(s)	1063
EaeI	YGGCCR	3 cut(s)	672, 993, 1933
Eam1104I	CTCTTC	1 cut(s)	1017
EarI	CTCTTC	1 cut(s)	1017
EciI	GGCGGA	1 cut(s)	1630
Ecl136II	GAGCTC	1 cut(s)	628
Eco130I	CCWWGG	4 cut(s)	990, 1460, 1790, 1936
Eco24I	GRGCYC	4 cut(s)	188, 630, 1784, 1799
Eco47I	GGWCC	2 cut(s)	377, 1787
Eco53kI	GAGCTC	1 cut(s)	628
Eco57I	CTGAAG	2 cut(s)	939, 1791
Eco88I	CYCGRG	1 cut(s)	777
EcoICRI	GAGCTC	1 cut(s)	628
EcoNI	CCTNNNNNAGG	2 cut(s)	547, 1809
EcoRII	CCWGG	4 cut(s)	446, 600, 995, 1417
EcoT14I	CCWWGG	4 cut(s)	990, 1460, 1790, 1936
EcoT22I	ATGCAT	1 cut(s)	1180
EcoT38I	GRGCYC	4 cut(s)	188, 630, 1784, 1799
ErhI	CCWWGG	4 cut(s)	990, 1460, 1790, 1936
FaeI	CATG	7 cut(s)	257, 643, 827, 994, 1234, 1295, 1464
FalI	AAGNNNNNCTT	2 cut(s)	756, 788
FaqI	GGGAC	2 cut(s)	861, 1444
FatI	CATG	7 cut(s)	253, 639, 823, 990, 1230, 1291, 1460
FauI	CCCGC	1 cut(s)	1335
FauNDI	CATATG	1 cut(s)	741
Fnu4HI	GCNGC	9 cut(s)	9, 176, 465, 644, 1135, 1269, 1605, 1900, 1929
FokI	GGATG	5 cut(s)	378, 723, 814, 1147, 1288
FriOI	GRGCYC	4 cut(s)	188, 630, 1784, 1799
Fsp4HI	GCNGC	9 cut(s)	9, 176, 465, 644, 1135, 1269, 1605, 1900, 1929
GlaI	GCGC	1 cut(s)	1467
GluI	GCNGC	9 cut(s)	9, 176, 465, 644, 1135, 1269, 1605, 1900, 1929
GsaI	CCCAGC	1 cut(s)	1411
GsuI	CTGGAG	2 cut(s)	777, 1944
HaeIII	GGCC	3 cut(s)	674, 995, 1935
HapII	CCGG	6 cut(s)	432, 1205, 1427, 1613, 1785, 1932
HgaI	GACGC	2 cut(s)	220, 888
HhaI	GCGC	1 cut(s)	1468
Hin1I	GRCGYC	1 cut(s)	14
Hin1II	CATG	7 cut(s)	257, 643, 827, 994, 1234, 1295, 1464
Hin6I	GCGC	1 cut(s)	1466
HinP1I	GCGC	1 cut(s)	1466
HindIII	AAGCTT	1 cut(s)	868
HinfI	GANTC	6 cut(s)	113, 353, 583, 605, 929, 944
HpaII	CCGG	6 cut(s)	432, 1205, 1427, 1613, 1785, 1932
HphI	GGTGA	4 cut(s)	241, 254, 1135, 1602
Hpy166II	GTNNAC	2 cut(s)	52, 655
Hpy188I	TCNGA	4 cut(s)	75, 460, 1245, 1857
Hpy188III	TCNNGA	9 cut(s)	83, 329, 340, 432, 546, 785, 1003, 1364, 1923
Hpy8I	GTNNAC	2 cut(s)	52, 655
Hpy99I	CGWCG	5 cut(s)	16, 19, 22, 31, 64
HpyAV	CCTTC	5 cut(s)	543, 1047, 1250, 1413, 1815
HpyCH4III	ACNGT	7 cut(s)	239, 668, 1071, 1336, 1435, 1503, 1599
HpyCH4IV	ACGT	2 cut(s)	14, 1835
HpyF10VI	GCNNNNNNNGC	8 cut(s)	44, 461, 497, 811, 1046, 1134, 1703, 1808
HpyF3I	CTNAG	7 cut(s)	267, 396, 422, 587, 631, 1244, 1530
HpySE526I	ACGT	2 cut(s)	14, 1835
Hsp92I	GRCGYC	1 cut(s)	14
Hsp92II	CATG	7 cut(s)	257, 643, 827, 994, 1234, 1295, 1464
HspAI	GCGC	1 cut(s)	1466
Kpn2I	TCCGGA	1 cut(s)	431
KroI	GCCGGC	1 cut(s)	1931
KroNI	GCCGGC	1 cut(s)	1933
Kzo9I	GATC	7 cut(s)	67, 735, 999, 1144, 1192, 1233, 1852
LmnI	GCTCC	7 cut(s)	472, 625, 697, 1171, 1366, 1794, 1925
Lsp1109I	GCAGC	8 cut(s)	187, 476, 630, 1146, 1255, 1616, 1911, 1915
LweI	GCATC	5 cut(s)	464, 792, 817, 1310, 1352
MaeII	ACGT	2 cut(s)	14, 1835
MaeIII	GTNAC	4 cut(s)	662, 941, 1352, 1545
MalI	GATC	7 cut(s)	69, 737, 1001, 1146, 1194, 1235, 1854
MbiI	CCGCTC	1 cut(s)	1166
MboI	GATC	7 cut(s)	67, 735, 999, 1144, 1192, 1233, 1852
MboII	GAAGA	9 cut(s)	24, 62, 244, 776, 1025, 1031, 1034, 1040, 1862
MflI	RGATCY	3 cut(s)	999, 1144, 1852
MhlI	GDGCHC	5 cut(s)	188, 630, 1130, 1784, 1799
MlsI	TGGCCA	2 cut(s)	674, 995
MluNI	TGGCCA	2 cut(s)	674, 995
MlyI	GAGTC	2 cut(s)	347, 938
MmeI	TCCRAC	3 cut(s)	1329, 1516, 1531
Mox20I	TGGCCA	2 cut(s)	674, 995
Mph1103I	ATGCAT	1 cut(s)	1180
MroI	TCCGGA	1 cut(s)	431
MroNI	GCCGGC	1 cut(s)	1931
MroXI	GAANNNNTTC	1 cut(s)	768
MscI	TGGCCA	2 cut(s)	674, 995
MseI	TTAA	5 cut(s)	162, 294, 1062, 1209, 1887
Msp20I	TGGCCA	2 cut(s)	674, 995
MspA1I	CMGCKG	1 cut(s)	1268
MspI	CCGG	6 cut(s)	432, 1205, 1427, 1613, 1785, 1932
MspR9I	CCNGG	7 cut(s)	448, 602, 997, 1419, 1428, 1614, 1785
Mva1269I	GAATGC	1 cut(s)	534
MvaI	CCWGG	4 cut(s)	448, 602, 997, 1419
MwoI	GCNNNNNNNGC	8 cut(s)	44, 461, 497, 811, 1046, 1134, 1703, 1808
NaeI	GCCGGC	1 cut(s)	1933
NciI	CCSGG	3 cut(s)	1428, 1614, 1785
NcoI	CCATGG	2 cut(s)	990, 1460
NdeI	CATATG	1 cut(s)	741
NdeII	GATC	7 cut(s)	67, 735, 999, 1144, 1192, 1233, 1852
NgoMIV	GCCGGC	1 cut(s)	1931
NheI	GCTAGC	1 cut(s)	1045
NlaIII	CATG	7 cut(s)	257, 643, 827, 994, 1234, 1295, 1464
NlaIV	GGNNCC	6 cut(s)	988, 1001, 1077, 1338, 1368, 1796
NmuCI	GTSAC	3 cut(s)	662, 941, 1545
NsiI	ATGCAT	1 cut(s)	1180
NspI	RCATGY	1 cut(s)	643
PcsI	WCGNNNNNNNCGW	3 cut(s)	23, 26, 35
PctI	GAATGC	1 cut(s)	534
PdiI	GCCGGC	1 cut(s)	1933
PdmI	GAANNNNTTC	1 cut(s)	768
PfeI	GAWTC	4 cut(s)	113, 583, 605, 929
PflFI	GACNNNGTC	1 cut(s)	16
PflMI	CCANNNNNTGG	1 cut(s)	1942
PfoI	TCCNGGA	1 cut(s)	1417
PinAI	ACCGGT	1 cut(s)	1204
PkrI	GCNGC	9 cut(s)	10, 177, 466, 645, 1136, 1270, 1606, 1901, 1930
PleI	GAGTC	2 cut(s)	347, 938
PpsI	GAGTC	2 cut(s)	347, 938
Ppu21I	YACGTR	1 cut(s)	1836
PshAI	GACNNNNGTC	1 cut(s)	948
Psp124BI	GAGCTC	1 cut(s)	630
Psp6I	CCWGG	4 cut(s)	446, 600, 995, 1417
PspFI	CCCAGC	1 cut(s)	1407
PspGI	CCWGG	4 cut(s)	446, 600, 995, 1417
PspN4I	GGNNCC	6 cut(s)	988, 1001, 1077, 1338, 1368, 1796
PspPI	GGNCC	2 cut(s)	377, 1787
PsuI	RGATCY	3 cut(s)	999, 1144, 1852
PsyI	GACNNNGTC	1 cut(s)	16
PvuII	CAGCTG	1 cut(s)	1268
RsaI	GTAC	3 cut(s)	401, 438, 1838
RsaNI	GTAC	3 cut(s)	400, 437, 1837
SacI	GAGCTC	1 cut(s)	630
SaqAI	TTAA	5 cut(s)	162, 294, 1062, 1209, 1887
SatI	GCNGC	9 cut(s)	9, 176, 465, 644, 1135, 1269, 1605, 1900, 1929
Sau3AI	GATC	7 cut(s)	67, 735, 999, 1144, 1192, 1233, 1852
Sau96I	GGNCC	2 cut(s)	377, 1787
ScaI	AGTACT	1 cut(s)	401
SchI	GAGTC	2 cut(s)	347, 938
ScrFI	CCNGG	7 cut(s)	448, 602, 997, 1419, 1428, 1614, 1785
SduI	GDGCHC	5 cut(s)	188, 630, 1130, 1784, 1799
SfaNI	GCATC	5 cut(s)	464, 792, 817, 1310, 1352
SfcI	CTRYAG	2 cut(s)	570, 831
SinI	GGWCC	2 cut(s)	377, 1787
SmlI	CTYRAG	3 cut(s)	513, 544, 858
SmoI	CTYRAG	3 cut(s)	513, 544, 858
SsiI	CCGC	4 cut(s)	1164, 1328, 1511, 1641
SspI	AATATT	1 cut(s)	474
SstI	GAGCTC	1 cut(s)	630
StyD4I	CCNGG	7 cut(s)	446, 600, 995, 1417, 1426, 1612, 1783
StyI	CCWWGG	4 cut(s)	990, 1460, 1790, 1936
TaaI	ACNGT	7 cut(s)	239, 668, 1071, 1336, 1435, 1503, 1599
TaiI	ACGT	2 cut(s)	17, 1838
TaqI	TCGA	3 cut(s)	555, 1051, 1236
TatI	WGTACW	2 cut(s)	399, 436
TfiI	GAWTC	4 cut(s)	113, 583, 605, 929
Tru1I	TTAA	5 cut(s)	162, 294, 1062, 1209, 1887
Tru9I	TTAA	5 cut(s)	162, 294, 1062, 1209, 1887
TseFI	GTSAC	3 cut(s)	662, 941, 1545
TseI	GCWGC	9 cut(s)	8, 175, 464, 643, 1134, 1268, 1604, 1899, 1928
Tsp45I	GTSAC	3 cut(s)	662, 941, 1545
TspDTI	ATGAA	7 cut(s)	110, 573, 699, 777, 1026, 1047, 1763
TspGWI	ACGGA	3 cut(s)	106, 540, 1782
Tth111I	GACNNNGTC	1 cut(s)	16
Van91I	CCANNNNNTGG	1 cut(s)	1942
VpaK11BI	GGWCC	2 cut(s)	377, 1787
XagI	CCTNNNNNAGG	2 cut(s)	547, 1809
XapI	RAATTY	1 cut(s)	1034
XceI	RCATGY	1 cut(s)	643
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1415
XmaJI	CCTAGG	1 cut(s)	1790
XmnI	GAANNNNTTC	1 cut(s)	768
ZraI	GACGTC	1 cut(s)	15
ZrmI	AGTACT	1 cut(s)	401
Zsp2I	ATGCAT	1 cut(s)	1180

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.