MD12G1193600.v1.1

Protein of unknown function (DUF1191)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0014156
Physical Location & Seq
Reverse (-)
27515828 .. 27518397
2570 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1193600.v1.1.491

Sequence Viewer

Length: 969 bp
ATGGGGCTTCTTCTTCGAAGTCTCGCGATGCTGTTCATTGTGATATGTTTCTCCTGGCTGCCTGATGTTTCTGCGCAATCCTCAGGAGGCTCAGCACGCGGTCTTGATGCGCTTCTTCAAGATTATGCTTACAGGTCTTTTGTTAATCCAAAGACAGGTGTTATATTTAATGGCAGTGTTCCCTTGAATTTGACTGGAATTCAGATTGCAGCTATGAGGCTGAGGAGTGGTAGCTTGTACAGAAGAGGTGTTAACATGTACAAAGAGTTTGAGATCCCTAAGGGTGTCACTGAGAGTCCGTATGTGGAGAGGCTTGTTTTGGTGTACCAGAACCTGGGCAATTGGTCAATGGTGTATTACCCGTTACAGGGTTACTCTTACTTAGCTCCATTGATCGGTCTTCTTGCTTATGATGCTGGCAACCTATCAGCAAAAAATCTGCCAGAATTGGTTATCAGGGCATCTGGTGATCCAATAATGATCAAATTCCAGGATGTGAAACCGACTCCTGCTGGGACAGTGGCACAGTGTGTTTTGTTTGATTTGGATGGTTCAATCAATTTCAGCAATGTGGAATCAGGCAATATGTGTTCAGCAGTTCAACAAGGGCACTTCTCCATAGCGGTGAAGTCCATTGCTCCTTCTCCAGCACCAGTCTCTCCAAGTCCCACCCCTGCTGGTGAAAGTCCAAACGTGGCCCCAGTGCCTAGTGGTAGACGAAAGAAGAAGAGTAATTCGAAAGTGTGGATAATTGTTGGGTCTGTGCTGGGTGGTTTAGCGTTGTTGGCATTGTTGTCGTTCTTAGTACTTTGGGGAAAAAAGTACAGGGAAAGGAAGAATTTGCAACGGATGGAGACGGCAGCAGATGTAGGAGAAGCCTTGCAAATGACCTCAGTTGGAGATGCAAAGGCACCTGCAGCAACATTTACGCGAACACAACCGACTATCGAGACTGAATATGTTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1191

Protein Analysis

323

Amino Acids

34.74

Weight (kDa)

9.33

Isoelectric Point (pI)

44.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1191 PF06697 31 - 210 6.6e-70 Protein of unknown function (DUF1191)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014156)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G08600
fragaria_vesca FvH4_3g04260 FvH4_6g09410
malus_domestica MD04G1178000.v1.1 MD12G1193600.v1.1
prunus_persica Prupe.6G297900_v2.0.a1
pyrus_communis pycom04g15700 pycom12g17980
rosa_chinensis RchiOBHm_Chr3g0459851
rosa_laevigata RLG00000025056
rosa_multiflora Rmu_sc0000240.1_g000040
rosa_roxburghii Rroxscaffold_6G00419960
rosa_rugosa Rorug03G0038100
rosa_samantha Rh3AG097000 Rh3BG099800 Rh3CG101600 Rh3DG101200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 922
Acc16I TGCGCA 1 cut(s) 75
Acc36I ACCTGC 1 cut(s) 922
AccB1I GGYRCC 1 cut(s) 910
AccB7I CCANNNNNTGG 1 cut(s) 334
AccI GTMKAC 1 cut(s) 715
AccII CGCG 3 cut(s) 26, 99, 931
AciI CCGC 2 cut(s) 99, 623
AclWI GGATC 2 cut(s) 268, 464
AcsI RAATTY 4 cut(s) 187, 198, 485, 838
AdeI CACNNNGTG 1 cut(s) 530
AfaI GTAC 5 cut(s) 239, 260, 326, 807, 824
AfiI CCNNNNNNNGG 5 cut(s) 155, 334, 367, 368, 395
AflIII ACRYGT 1 cut(s) 255
AgsI TTSAA 4 cut(s) 119, 187, 555, 602
AjnI CCWGG 3 cut(s) 53, 333, 489
AluBI AGCT 3 cut(s) 212, 234, 386
AluI AGCT 3 cut(s) 212, 234, 386
Alw26I GTCTC 4 cut(s) 26, 661, 848, 944
AlwI GGATC 2 cut(s) 268, 464
AlwNI CAGNNNCTG 1 cut(s) 334
AoxI GGCC 1 cut(s) 696
ApeKI GCWGC 4 cut(s) 58, 209, 860, 917
ApoI RAATTY 4 cut(s) 187, 198, 485, 838
Asp700I GAANNNNTTC 1 cut(s) 960
AspLEI GCGC 2 cut(s) 76, 112
AspS9I GGNCC 1 cut(s) 697
AsuHPI GGTGA 3 cut(s) 479, 637, 692
AsuII TTCGAA 2 cut(s) 16, 737
AxyI CCTNAGG 2 cut(s) 82, 279
BaeGI GKGCMC 1 cut(s) 612
BanI GGYRCC 1 cut(s) 910
BbsI GAAGAC 1 cut(s) 392
BbvCI CCTCAGC 1 cut(s) 221
BbvI GCAGC 4 cut(s) 45, 221, 872, 929
BccI CCATC 2 cut(s) 542, 844
BceAI ACGGC 1 cut(s) 873
BcgI CGANNNNNNTGC 2 cut(s) 777, 811
BciT130I CCWGG 3 cut(s) 55, 335, 491
BclI TGATCA 1 cut(s) 480
BcoDI GTCTC 4 cut(s) 26, 661, 848, 944
BfaI CTAG 1 cut(s) 708
BfmI CTRYAG 1 cut(s) 915
BfuAI ACCTGC 1 cut(s) 922
BisI GCNGC 4 cut(s) 59, 210, 861, 918
BlpI GCTNAGC 1 cut(s) 91
BlsI GCNGC 4 cut(s) 60, 211, 862, 919
BmcAI AGTACT 1 cut(s) 807
Bme1390I CCNGG 3 cut(s) 55, 335, 491
BmgT120I GGNCC 1 cut(s) 697
BmiI GGNNCC 2 cut(s) 699, 912
BmrFI CCNGG 3 cut(s) 55, 335, 491
BmrI ACTGGG 1 cut(s) 695
BmsI GCATC 5 cut(s) 18, 97, 403, 470, 892
BmuI ACTGGG 1 cut(s) 695
BpiI GAAGAC 1 cut(s) 392
BpmI CTGGAG 1 cut(s) 630
Bpu10I CCTNAGC 1 cut(s) 221
Bpu1102I GCTNAGC 1 cut(s) 91
Bpu14I TTCGAA 2 cut(s) 16, 737
BsaJI CCNNGG 1 cut(s) 334
Bsc4I CCNNNNNNNGG 5 cut(s) 155, 334, 367, 368, 395
Bse1I ACTGG 3 cut(s) 199, 653, 701
Bse21I CCTNAGG 2 cut(s) 82, 279
Bse3DI GCAATG 2 cut(s) 574, 633
BseBI CCWGG 3 cut(s) 55, 335, 491
BseDI CCNNGG 1 cut(s) 334
BseGI GGATG 3 cut(s) 499, 553, 855
BseLI CCNNNNNNNGG 5 cut(s) 155, 334, 367, 368, 395
BseMI GCAATG 2 cut(s) 574, 633
BseMII CTCAG 5 cut(s) 96, 105, 212, 282, 906
BseNI ACTGG 3 cut(s) 199, 653, 701
BseRI GAGGAG 1 cut(s) 238
BseSI GKGCMC 1 cut(s) 612
BseXI GCAGC 4 cut(s) 45, 221, 872, 929
BseYI CCCAGC 2 cut(s) 512, 766
Bsh1236I CGCG 3 cut(s) 26, 99, 931
BshFI GGCC 1 cut(s) 698
BshNI GGYRCC 1 cut(s) 910
BslFI GGGAC 2 cut(s) 529, 651
BslI CCNNNNNNNGG 5 cut(s) 155, 334, 367, 368, 395
BsmAI GTCTC 4 cut(s) 26, 661, 848, 944
BsmBI CGTCTC 1 cut(s) 848
BsmFI GGGAC 2 cut(s) 529, 651
BsnI GGCC 1 cut(s) 698
Bsp119I TTCGAA 2 cut(s) 16, 737
Bsp1286I GDGCHC 1 cut(s) 612
Bsp1407I TGTACA 2 cut(s) 237, 258
Bsp143I GATC 4 cut(s) 273, 393, 469, 480
Bsp1720I GCTNAGC 1 cut(s) 91
Bsp68I TCGCGA 1 cut(s) 26
BspACI CCGC 2 cut(s) 99, 623
BspANI GGCC 1 cut(s) 698
BspCNI CTCAG 5 cut(s) 95, 104, 213, 283, 905
BspFNI CGCG 3 cut(s) 26, 99, 931
BspLI GGNNCC 2 cut(s) 699, 912
BspMAI CTGCAG 1 cut(s) 919
BspMI ACCTGC 1 cut(s) 922
BspPI GGATC 2 cut(s) 268, 464
BspT104I TTCGAA 2 cut(s) 16, 737
BspT107I GGYRCC 1 cut(s) 910
BsrDI GCAATG 2 cut(s) 574, 633
BsrGI TGTACA 2 cut(s) 237, 258
BsrI ACTGG 3 cut(s) 199, 653, 701
BssECI CCNNGG 1 cut(s) 334
BssMI GATC 4 cut(s) 273, 393, 469, 480
Bst2UI CCWGG 3 cut(s) 55, 335, 491
Bst4CI ACNGT 2 cut(s) 520, 528
Bst6I CTCTTC 2 cut(s) 238, 722
BstAUI TGTACA 2 cut(s) 237, 258
BstBI TTCGAA 2 cut(s) 16, 737
BstC8I GCNNGC 2 cut(s) 97, 418
BstDEI CTNAG 8 cut(s) 82, 91, 221, 279, 291, 382, 802, 892
BstF5I GGATG 3 cut(s) 499, 553, 855
BstFNI CGCG 3 cut(s) 26, 99, 931
BstHHI GCGC 2 cut(s) 76, 112
BstKTI GATC 4 cut(s) 276, 396, 472, 483
BstMAI GTCTC 4 cut(s) 26, 661, 848, 944
BstMBI GATC 4 cut(s) 273, 393, 469, 480
BstMWI GCNNNNNNNGC 4 cut(s) 96, 413, 785, 917
BstNI CCWGG 3 cut(s) 55, 335, 491
BstNSI RCATGY 1 cut(s) 259
BstSCI CCNGG 3 cut(s) 53, 333, 489
BstSFI CTRYAG 1 cut(s) 915
BstSLI GKGCMC 1 cut(s) 612
BstUI CGCG 3 cut(s) 26, 99, 931
BstV1I GCAGC 4 cut(s) 45, 221, 872, 929
BstV2I GAAGAC 1 cut(s) 392
BstX2I RGATCY 1 cut(s) 273
BstYI RGATCY 1 cut(s) 273
Bsu36I CCTNAGG 2 cut(s) 82, 279
BsuRI GGCC 1 cut(s) 698
BtgZI GCGATG 1 cut(s) 41
BtsCI GGATG 3 cut(s) 499, 553, 855
BtsI GCAGTG 1 cut(s) 181
BtsIMutI CAGTG 5 cut(s) 181, 288, 525, 533, 708
BtuMI TCGCGA 1 cut(s) 26
BveI ACCTGC 1 cut(s) 922
Cac8I GCNNGC 2 cut(s) 97, 418
CaiI CAGNNNCTG 1 cut(s) 334
CfoI GCGC 2 cut(s) 76, 112
Cfr13I GGNCC 1 cut(s) 697
Csp6I GTAC 5 cut(s) 238, 259, 325, 806, 823
CviAII CATG 1 cut(s) 256
CviQI GTAC 5 cut(s) 238, 259, 325, 806, 823
DdeI CTNAG 8 cut(s) 82, 91, 221, 279, 291, 382, 802, 892
DpnI GATC 4 cut(s) 275, 395, 471, 482
DpnII GATC 4 cut(s) 273, 393, 469, 480
DraIII CACNNNGTG 1 cut(s) 530
Eam1104I CTCTTC 2 cut(s) 238, 722
EarI CTCTTC 2 cut(s) 238, 722
Eco81I CCTNAGG 2 cut(s) 82, 279
EcoRI GAATTC 1 cut(s) 198
EcoRII CCWGG 3 cut(s) 53, 333, 489
Esp3I CGTCTC 1 cut(s) 848
FaeI CATG 1 cut(s) 259
FaqI GGGAC 2 cut(s) 529, 651
FatI CATG 1 cut(s) 255
FbaI TGATCA 1 cut(s) 480
FblI GTMKAC 1 cut(s) 715
Fnu4HI GCNGC 4 cut(s) 59, 210, 861, 918
FokI GGATG 3 cut(s) 506, 560, 862
Fsp4HI GCNGC 4 cut(s) 59, 210, 861, 918
FspBI CTAG 1 cut(s) 708
FspI TGCGCA 1 cut(s) 75
GlaI GCGC 2 cut(s) 75, 111
GluI GCNGC 4 cut(s) 59, 210, 861, 918
GsaI CCCAGC 2 cut(s) 516, 770
GsuI CTGGAG 1 cut(s) 630
HaeIII GGCC 1 cut(s) 698
HhaI GCGC 2 cut(s) 76, 112
Hin1II CATG 1 cut(s) 259
Hin6I GCGC 2 cut(s) 74, 110
HinP1I GCGC 2 cut(s) 74, 110
HincII GTYRAC 1 cut(s) 253
HindII GTYRAC 1 cut(s) 253
HinfI GANTC 3 cut(s) 295, 505, 575
HpaI GTTAAC 1 cut(s) 253
HphI GGTGA 3 cut(s) 479, 637, 692
Hpy166II GTNNAC 3 cut(s) 253, 325, 716
Hpy188I TCNGA 1 cut(s) 204
Hpy188III TCNNGA 5 cut(s) 25, 84, 104, 119, 949
Hpy8I GTNNAC 3 cut(s) 253, 325, 716
HpyAV CCTTC 1 cut(s) 651
HpyCH4III ACNGT 2 cut(s) 520, 528
HpyCH4IV ACGT 1 cut(s) 693
HpyCH4V TGCA 5 cut(s) 209, 844, 883, 905, 917
HpyF10VI GCNNNNNNNGC 4 cut(s) 96, 413, 785, 917
HpyF3I CTNAG 8 cut(s) 82, 91, 221, 279, 291, 382, 802, 892
HpySE526I ACGT 1 cut(s) 693
Hsp92II CATG 1 cut(s) 259
HspAI GCGC 2 cut(s) 74, 110
Ksp22I TGATCA 1 cut(s) 480
KspAI GTTAAC 1 cut(s) 253
Kzo9I GATC 4 cut(s) 273, 393, 469, 480
LmnI GCTCC 2 cut(s) 391, 643
Lsp1109I GCAGC 4 cut(s) 45, 221, 872, 929
LweI GCATC 5 cut(s) 18, 97, 403, 470, 892
MaeI CTAG 1 cut(s) 708
MaeII ACGT 1 cut(s) 693
MaeIII GTNAC 3 cut(s) 286, 363, 371
MalI GATC 4 cut(s) 275, 395, 471, 482
MboI GATC 4 cut(s) 273, 393, 469, 480
MboII GAAGA 7 cut(s) 5, 107, 255, 392, 736, 739, 847
MfeI CAATTG 1 cut(s) 340
MflI RGATCY 1 cut(s) 273
MhlI GDGCHC 1 cut(s) 612
MluCI AATT 9 cut(s) 187, 198, 340, 446, 485, 559, 733, 750, 838
MlyI GAGTC 2 cut(s) 304, 499
MmeI TCCRAC 1 cut(s) 877
MnlI CCTC 7 cut(s) 80, 91, 210, 216, 239, 303, 901
MroXI GAANNNNTTC 1 cut(s) 960
MseI TTAA 3 cut(s) 144, 168, 252
MslI CAYNNNNRTG 1 cut(s) 623
MspR9I CCNGG 3 cut(s) 55, 335, 491
MunI CAATTG 1 cut(s) 340
MvaI CCWGG 3 cut(s) 55, 335, 491
MvnI CGCG 3 cut(s) 26, 99, 931
MwoI GCNNNNNNNGC 4 cut(s) 96, 413, 785, 917
NdeII GATC 4 cut(s) 273, 393, 469, 480
NlaIII CATG 1 cut(s) 259
NlaIV GGNNCC 2 cut(s) 699, 912
NmuCI GTSAC 1 cut(s) 286
NruI TCGCGA 1 cut(s) 26
NsbI TGCGCA 1 cut(s) 75
NspI RCATGY 1 cut(s) 259
NspV TTCGAA 2 cut(s) 16, 737
PaqCI CACCTGC 1 cut(s) 922
PciI ACATGT 1 cut(s) 255
PdmI GAANNNNTTC 1 cut(s) 960
PfeI GAWTC 1 cut(s) 575
PflMI CCANNNNNTGG 1 cut(s) 334
PfoI TCCNGGA 1 cut(s) 489
PkrI GCNGC 4 cut(s) 60, 211, 862, 919
PleI GAGTC 2 cut(s) 303, 499
PpsI GAGTC 2 cut(s) 303, 499
PscI ACATGT 1 cut(s) 255
Psp6I CCWGG 3 cut(s) 53, 333, 489
PspFI CCCAGC 2 cut(s) 512, 766
PspGI CCWGG 3 cut(s) 53, 333, 489
PspN4I GGNNCC 2 cut(s) 699, 912
PspPI GGNCC 1 cut(s) 697
PstI CTGCAG 1 cut(s) 919
PstNI CAGNNNCTG 1 cut(s) 334
PsuI RGATCY 1 cut(s) 273
RruI TCGCGA 1 cut(s) 26
RsaI GTAC 5 cut(s) 239, 260, 326, 807, 824
RsaNI GTAC 5 cut(s) 238, 259, 325, 806, 823
RseI CAYNNNNRTG 1 cut(s) 623
SaqAI TTAA 3 cut(s) 144, 168, 252
SatI GCNGC 4 cut(s) 59, 210, 861, 918
Sau3AI GATC 4 cut(s) 273, 393, 469, 480
Sau96I GGNCC 1 cut(s) 697
ScaI AGTACT 1 cut(s) 807
SchI GAGTC 2 cut(s) 304, 499
ScrFI CCNGG 3 cut(s) 55, 335, 491
SduI GDGCHC 1 cut(s) 612
SfaNI GCATC 5 cut(s) 18, 97, 403, 470, 892
SfcI CTRYAG 1 cut(s) 915
SfuI TTCGAA 2 cut(s) 16, 737
SmiMI CAYNNNNRTG 1 cut(s) 623
Sse9I AATT 9 cut(s) 187, 198, 340, 446, 485, 559, 733, 750, 838
SsiI CCGC 2 cut(s) 99, 623
SspMI CTAG 1 cut(s) 708
StyD4I CCNGG 3 cut(s) 53, 333, 489
TaaI ACNGT 2 cut(s) 520, 528
TaiI ACGT 1 cut(s) 696
TaqI TCGA 3 cut(s) 16, 737, 948
TaqII GACCGA 1 cut(s) 386
TasI AATT 9 cut(s) 187, 198, 340, 446, 485, 559, 733, 750, 838
TatI WGTACW 4 cut(s) 237, 258, 805, 822
TfiI GAWTC 1 cut(s) 575
Tru1I TTAA 3 cut(s) 144, 168, 252
Tru9I TTAA 3 cut(s) 144, 168, 252
TscAI CASTG 5 cut(s) 181, 295, 525, 533, 708
TseFI GTSAC 1 cut(s) 286
TseI GCWGC 4 cut(s) 58, 209, 860, 917
Tsp45I GTSAC 1 cut(s) 286
TspDTI ATGAA 1 cut(s) 25
TspGWI ACGGA 2 cut(s) 288, 862
TspRI CASTG 5 cut(s) 181, 295, 525, 533, 708
Van91I CCANNNNNTGG 1 cut(s) 334
XapI RAATTY 4 cut(s) 187, 198, 485, 838
XceI RCATGY 1 cut(s) 259
XmiI GTMKAC 1 cut(s) 715
XmnI GAANNNNTTC 1 cut(s) 960
XspI CTAG 1 cut(s) 708
ZrmI AGTACT 1 cut(s) 807
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.