MD12G1195700.v1.1

Belongs to the bacterial ribosomal protein bL17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0015209
Physical Location & Seq
Reverse (-)
27678587 .. 27689255
10669 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1195700.v1.1.491

Sequence Viewer

Length: 669 bp
ATGGCGATGACAATGGCATTGGCGACGGCTACCGGAGTAGCAGAAACCAGCAGATGGAGCATGGCGTCTCTGAAGGCGGCGCTGCCATCCCCGCCGGAGAGGTTCACCGTTCCTACCGCTTCTTCTTCTTCTTCTTCTTCGGCAACGTGCGGGCATCGGAGCCTGCGCCTCAGCGTCGGACTCCGCAAACCCGAGCTCCAATTCGGGTCATTCTCGGGTCTCTCTCCCCTGAACCCGCTCCTCTCCCTCGGCCTCTCCGAGTCCACGAGTTTTGAACACAACTTCACCATCATTGATAATGGTGGTCGTATTTCTGCCATGAGACATGGCAGGAAAGTTCCTAAGCTCAACAGGCCTCCTGACCAGAGGAAAGCACTTCTTCGAGGCCTCACAACTCAGCTCCTTAAACATGGACGCATCAAAACTACTCGAGCAAGGGCAAGTGCTATGAGGAAGTACGTGGACAAGATGATCACACTGGCCAAGGATGGATCTCTCCATAAGAGGAGACAAGCACTGGGATACATCTACGAGAAACAGATTGTCCATGCTTTGTTTGCAGAGGTACCTGACAGATATGGGGAGCGGAATGGTGGCTATACAAGAATCATCAGAACTCTGCCAAGGCGAGGGGACAATGCACCCATGGCATACATTGAACTTGTGTAG

Protein Analysis

223

Amino Acids

24.45

Weight (kDa)

10.94

Isoelectric Point (pI)

45.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L17 PF01196 126 - 222 7.5e-38 Ribosomal protein L17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0015209)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G54210
fragaria_vesca FvH4_6g09150
malus_domestica MD12G1195700.v1.1
prunus_persica Prupe.6G300200_v2.0.a1
pyrus_communis pycom12g18190
rosa_chinensis RchiOBHm_Chr3g0459361
rosa_laevigata RLG00000025087
rosa_multiflora Rmu_sc0006944.1_g000001
rosa_roxburghii Rroxscaffold_6G00420230
rosa_rugosa Rorug03G0035800.1 Rorug03G0036200
rosa_samantha Rh3AG093800 Rh3BG096700 Rh3CG097900 Rh3DG097400
rosa_wichuraiana Rw3G008070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 565
AccB1I GGYRCC 1 cut(s) 565
AccB7I CCANNNNNTGG 1 cut(s) 54
AccBSI CCGCTC 2 cut(s) 238, 586
AciI CCGC 7 cut(s) 77, 92, 117, 150, 184, 236, 586
AclWI GGATC 1 cut(s) 499
AcoI YGGCCR 1 cut(s) 480
AcuI CTGAAG 1 cut(s) 92
AcyI GRCGYC 1 cut(s) 65
AfaI GTAC 2 cut(s) 458, 567
AfiI CCNNNNNNNGG 2 cut(s) 54, 629
AgsI TTSAA 2 cut(s) 275, 659
AluBI AGCT 3 cut(s) 196, 346, 400
AluI AGCT 3 cut(s) 196, 346, 400
Alw21I GWGCWC 1 cut(s) 198
Alw26I GTCTC 4 cut(s) 72, 224, 316, 502
AlwI GGATC 1 cut(s) 499
Ama87I CYCGRG 3 cut(s) 191, 214, 429
AoxI GGCC 4 cut(s) 250, 353, 385, 480
ApeKI GCWGC 1 cut(s) 82
Asp718I GGTACC 1 cut(s) 565
AspLEI GCGC 2 cut(s) 82, 168
AsuHPI GGTGA 2 cut(s) 97, 277
AvaI CYCGRG 3 cut(s) 191, 214, 429
BalI TGGCCA 1 cut(s) 482
BanI GGYRCC 1 cut(s) 565
BanII GRGCYC 1 cut(s) 198
BauI CACGAG 1 cut(s) 265
Bbv12I GWGCWC 1 cut(s) 198
BbvCI CCTCAGC 1 cut(s) 170
BbvI GCAGC 1 cut(s) 69
BccI CCATC 4 cut(s) 48, 94, 296, 482
BceAI ACGGC 1 cut(s) 42
BciVI GTATCC 1 cut(s) 515
BclI TGATCA 1 cut(s) 471
BcoDI GTCTC 4 cut(s) 72, 224, 316, 502
BfoI RGCGCY 1 cut(s) 83
BfuI GTATCC 1 cut(s) 515
BisI GCNGC 2 cut(s) 78, 83
BlsI GCNGC 2 cut(s) 79, 84
BmeT110I CYCGRG 3 cut(s) 191, 214, 429
BmiI GGNNCC 2 cut(s) 161, 567
BmrI ACTGGG 1 cut(s) 527
BmsI GCATC 2 cut(s) 163, 426
BmuI ACTGGG 1 cut(s) 527
Bpu10I CCTNAGC 2 cut(s) 170, 342
BsaAI YACGTR 1 cut(s) 460
BsaHI GRCGYC 1 cut(s) 65
BsaI GGTCTC 1 cut(s) 224
BsaJI CCNNGG 4 cut(s) 247, 483, 623, 645
BsaWI WCCGGW 1 cut(s) 32
BsaXI ACNNNNNCTCC 2 cut(s) 180, 210
Bsc4I CCNNNNNNNGG 2 cut(s) 54, 629
Bse1I ACTGG 2 cut(s) 483, 522
BseDI CCNNGG 4 cut(s) 247, 483, 623, 645
BseGI GGATG 2 cut(s) 86, 493
BseLI CCNNNNNNNGG 2 cut(s) 54, 629
BseMII CTCAG 2 cut(s) 184, 410
BseNI ACTGG 2 cut(s) 483, 522
BseRI GAGGAG 2 cut(s) 230, 520
BseXI GCAGC 1 cut(s) 69
BshFI GGCC 4 cut(s) 252, 355, 387, 482
BshNI GGYRCC 1 cut(s) 565
BsiHKAI GWGCWC 1 cut(s) 198
BsiHKCI CYCGRG 3 cut(s) 191, 214, 429
BsiSI CCGG 2 cut(s) 33, 95
BslFI GGGAC 1 cut(s) 647
BslI CCNNNNNNNGG 2 cut(s) 54, 629
BsmAI GTCTC 4 cut(s) 72, 224, 316, 502
BsmBI CGTCTC 1 cut(s) 72
BsmFI GGGAC 1 cut(s) 647
BsnI GGCC 4 cut(s) 252, 355, 387, 482
Bso31I GGTCTC 1 cut(s) 224
BsoBI CYCGRG 3 cut(s) 191, 214, 429
Bsp1286I GDGCHC 1 cut(s) 198
Bsp143I GATC 2 cut(s) 471, 491
Bsp19I CCATGG 1 cut(s) 645
BspACI CCGC 7 cut(s) 77, 92, 117, 150, 184, 236, 586
BspANI GGCC 4 cut(s) 252, 355, 387, 482
BspCNI CTCAG 2 cut(s) 183, 409
BspLI GGNNCC 2 cut(s) 161, 567
BspPI GGATC 1 cut(s) 499
BspT107I GGYRCC 1 cut(s) 565
BspTNI GGTCTC 1 cut(s) 224
BsrBI CCGCTC 2 cut(s) 238, 586
BsrI ACTGG 2 cut(s) 483, 522
BssECI CCNNGG 4 cut(s) 247, 483, 623, 645
BssMI GATC 2 cut(s) 471, 491
BssNI GRCGYC 1 cut(s) 65
BssSI CACGAG 1 cut(s) 265
BssT1I CCWWGG 3 cut(s) 483, 623, 645
Bst2BI CACGAG 1 cut(s) 265
Bst4CI ACNGT 1 cut(s) 109
BstACI GRCGYC 1 cut(s) 65
BstBAI YACGTR 1 cut(s) 460
BstC8I GCNNGC 2 cut(s) 152, 164
BstDEI CTNAG 3 cut(s) 170, 342, 396
BstDSI CCRYGG 1 cut(s) 645
BstF5I GGATG 2 cut(s) 86, 493
BstH2I RGCGCY 1 cut(s) 83
BstHHI GCGC 2 cut(s) 82, 168
BstKTI GATC 2 cut(s) 474, 494
BstMAI GTCTC 4 cut(s) 72, 224, 316, 502
BstMBI GATC 2 cut(s) 471, 491
BstMWI GCNNNNNNNGC 5 cut(s) 57, 91, 352, 557, 647
BstV1I GCAGC 1 cut(s) 69
BstX2I RGATCY 1 cut(s) 491
BstYI RGATCY 1 cut(s) 491
BsuI GTATCC 1 cut(s) 515
BsuRI GGCC 4 cut(s) 252, 355, 387, 482
BtgI CCRYGG 1 cut(s) 645
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 2 cut(s) 86, 493
BtsIMutI CAGTG 2 cut(s) 476, 515
Cac8I GCNNGC 2 cut(s) 152, 164
CfoI GCGC 2 cut(s) 82, 168
CseI GACGC 3 cut(s) 54, 163, 423
Csp6I GTAC 2 cut(s) 457, 566
CspCI CAANNNNNGTGG 2 cut(s) 253, 288
CviAII CATG 6 cut(s) 61, 319, 326, 410, 548, 646
CviQI GTAC 2 cut(s) 457, 566
DdeI CTNAG 3 cut(s) 170, 342, 396
DpnI GATC 2 cut(s) 473, 493
DpnII GATC 2 cut(s) 471, 491
EaeI YGGCCR 1 cut(s) 480
Ecl136II GAGCTC 1 cut(s) 196
Eco130I CCWWGG 3 cut(s) 483, 623, 645
Eco147I AGGCCT 2 cut(s) 355, 387
Eco24I GRGCYC 1 cut(s) 198
Eco31I GGTCTC 1 cut(s) 224
Eco53kI GAGCTC 1 cut(s) 196
Eco57I CTGAAG 1 cut(s) 92
Eco88I CYCGRG 3 cut(s) 191, 214, 429
EcoICRI GAGCTC 1 cut(s) 196
EcoT14I CCWWGG 3 cut(s) 483, 623, 645
EcoT38I GRGCYC 1 cut(s) 198
ErhI CCWWGG 3 cut(s) 483, 623, 645
Esp3I CGTCTC 1 cut(s) 72
FaeI CATG 6 cut(s) 64, 322, 329, 413, 551, 649
FalI AAGNNNNNCTT 2 cut(s) 363, 395
FaqI GGGAC 1 cut(s) 647
FatI CATG 6 cut(s) 60, 318, 325, 409, 547, 645
FauI CCCGC 3 cut(s) 99, 143, 243
FbaI TGATCA 1 cut(s) 471
Fnu4HI GCNGC 2 cut(s) 78, 83
FokI GGATG 2 cut(s) 73, 500
FriOI GRGCYC 1 cut(s) 198
Fsp4HI GCNGC 2 cut(s) 78, 83
GlaI GCGC 2 cut(s) 81, 167
GluI GCNGC 2 cut(s) 78, 83
HaeII RGCGCY 1 cut(s) 83
HaeIII GGCC 4 cut(s) 252, 355, 387, 482
HapII CCGG 2 cut(s) 33, 95
HgaI GACGC 3 cut(s) 54, 163, 423
HhaI GCGC 2 cut(s) 82, 168
Hin1I GRCGYC 1 cut(s) 65
Hin1II CATG 6 cut(s) 64, 322, 329, 413, 551, 649
Hin6I GCGC 2 cut(s) 80, 166
HinP1I GCGC 2 cut(s) 80, 166
HinfI GANTC 3 cut(s) 180, 260, 606
HpaII CCGG 2 cut(s) 33, 95
HphI GGTGA 2 cut(s) 97, 277
Hpy166II GTNNAC 3 cut(s) 105, 264, 463
Hpy188I TCNGA 5 cut(s) 72, 159, 179, 259, 614
Hpy188III TCNNGA 1 cut(s) 359
Hpy8I GTNNAC 3 cut(s) 105, 264, 463
Hpy99I CGWCG 2 cut(s) 28, 179
HpyAV CCTTC 1 cut(s) 67
HpyCH4III ACNGT 1 cut(s) 109
HpyCH4IV ACGT 2 cut(s) 146, 459
HpyCH4V TGCA 2 cut(s) 560, 641
HpyF10VI GCNNNNNNNGC 5 cut(s) 57, 91, 352, 557, 647
HpyF3I CTNAG 3 cut(s) 170, 342, 396
HpySE526I ACGT 2 cut(s) 146, 459
Hsp92I GRCGYC 1 cut(s) 65
Hsp92II CATG 6 cut(s) 64, 322, 329, 413, 551, 649
HspAI GCGC 2 cut(s) 80, 166
KpnI GGTACC 1 cut(s) 569
Ksp22I TGATCA 1 cut(s) 471
Kzo9I GATC 2 cut(s) 471, 491
LmnI GCTCC 6 cut(s) 57, 159, 201, 243, 405, 583
Lsp1109I GCAGC 1 cut(s) 69
LweI GCATC 2 cut(s) 163, 426
MaeII ACGT 2 cut(s) 146, 459
MalI GATC 2 cut(s) 473, 493
MbiI CCGCTC 2 cut(s) 238, 586
MboI GATC 2 cut(s) 471, 491
MboII GAAGA 7 cut(s) 114, 117, 120, 123, 126, 129, 371
MflI RGATCY 1 cut(s) 491
MhlI GDGCHC 1 cut(s) 198
MlsI TGGCCA 1 cut(s) 482
MluCI AATT 1 cut(s) 200
MluNI TGGCCA 1 cut(s) 482
MlyI GAGTC 2 cut(s) 174, 269
MmeI TCCRAC 1 cut(s) 157
Mox20I TGGCCA 1 cut(s) 482
MscI TGGCCA 1 cut(s) 482
MseI TTAA 1 cut(s) 405
Msp20I TGGCCA 1 cut(s) 482
MspI CCGG 2 cut(s) 33, 95
MwoI GCNNNNNNNGC 5 cut(s) 57, 91, 352, 557, 647
NcoI CCATGG 1 cut(s) 645
NdeII GATC 2 cut(s) 471, 491
NlaIII CATG 6 cut(s) 64, 322, 329, 413, 551, 649
NlaIV GGNNCC 2 cut(s) 161, 567
NmeAIII GCCGAG 1 cut(s) 228
PaeR7I CTCGAG 1 cut(s) 429
PceI AGGCCT 2 cut(s) 355, 387
PcsI WCGNNNNNNNCGW 1 cut(s) 255
PfeI GAWTC 1 cut(s) 606
PflMI CCANNNNNTGG 1 cut(s) 54
PkrI GCNGC 2 cut(s) 79, 84
PleI GAGTC 2 cut(s) 174, 268
PpsI GAGTC 2 cut(s) 174, 268
Ppu21I YACGTR 1 cut(s) 460
Psp124BI GAGCTC 1 cut(s) 198
PspN4I GGNNCC 2 cut(s) 161, 567
PspXI VCTCGAGB 1 cut(s) 429
PsuI RGATCY 1 cut(s) 491
RsaI GTAC 2 cut(s) 458, 567
RsaNI GTAC 2 cut(s) 457, 566
SacI GAGCTC 1 cut(s) 198
SaqAI TTAA 1 cut(s) 405
SatI GCNGC 2 cut(s) 78, 83
Sau3AI GATC 2 cut(s) 471, 491
SchI GAGTC 2 cut(s) 174, 269
SduI GDGCHC 1 cut(s) 198
SetI ASST 8 cut(s) 104, 149, 198, 348, 402, 462, 567, 571
SfaNI GCATC 2 cut(s) 163, 426
Sfr274I CTCGAG 1 cut(s) 429
SlaI CTCGAG 1 cut(s) 429
SmlI CTYRAG 1 cut(s) 429
SmoI CTYRAG 1 cut(s) 429
Sse9I AATT 1 cut(s) 200
SseBI AGGCCT 2 cut(s) 355, 387
SsiI CCGC 7 cut(s) 77, 92, 117, 150, 184, 236, 586
SstI GAGCTC 1 cut(s) 198
StuI AGGCCT 2 cut(s) 355, 387
StyI CCWWGG 3 cut(s) 483, 623, 645
TaaI ACNGT 1 cut(s) 109
TaiI ACGT 2 cut(s) 149, 462
TaqI TCGA 2 cut(s) 382, 430
TasI AATT 1 cut(s) 200
TauI GCSGC 1 cut(s) 80
TfiI GAWTC 1 cut(s) 606
Tru1I TTAA 1 cut(s) 405
Tru9I TTAA 1 cut(s) 405
TscAI CASTG 2 cut(s) 483, 522
TseI GCWGC 1 cut(s) 82
TspRI CASTG 2 cut(s) 483, 522
Van91I CCANNNNNTGG 1 cut(s) 54
XhoI CTCGAG 1 cut(s) 429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.