pycom12g18190

Belongs to the bacterial ribosomal protein bL17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0015209
Physical Location & Seq
Reverse (-)
20402081 .. 20404435
2355 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g18190.1

Sequence Viewer

Length: 663 bp
ATGGCGACGATAATGGCATTGGCAACGGCTACCGGAGTAGCAGAAACCAGCAGATGGAGCATGGCGTCTCTGAAGGCGGCGCTGCCACCCCCGCCCGAGAGGTTCACCGTTCCTACCGCTTCTTCTTCTTCTTCGGCAACGTGCGGCCATCGGAGCCTGCGCCTCAGCGTCGGACTCCGCAAACCTGAGCTCCAATTCGGGTCTTTCTCGGGTCTCTCTCCCCTGAACCCGCTCCTGTCCCTCGGCCTCTCCGAGTCCACGAGTTTTGAACACAACTTCACCATCATTGATAATGGTGGTCGTATTTCTGCCATGAGACATGGCAGGAAAGTTCCTAAGCTCAACAGGCCTCCTGACCAGAGGAAAGCACTTCTTCGAGGCCTCACAACTCAGCTCCTTAAACACGGACGCATCAAAACTACTCGAGCAAGGGCAAGTGCTATGAGGAAGTACGTGGACAAGATGATCACACTGGCCAAGGATCGATCTCTCCATAAGAGGAGACAAGCGCTGGGATACATCTACGAGAAACAGATTGTCCATGCTTTGTTTGCAGAGGTACCTGACAGATATGGGGAGCGGAATGGTGGCTATACAAGAATCATCAGAACTCTGCCAAGGCGAGGGGACAATGCACCCATGGCATACATTGAACTCGTGTAG

Protein Analysis

221

Amino Acids

24.37

Weight (kDa)

11.06

Isoelectric Point (pI)

44.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L17 PF01196 124 - 220 2.2e-37 Ribosomal protein L17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015209)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G54210
fragaria_vesca FvH4_6g09150
malus_domestica MD12G1195700.v1.1
prunus_persica Prupe.6G300200_v2.0.a1
pyrus_communis pycom12g18190
rosa_chinensis RchiOBHm_Chr3g0459361
rosa_laevigata RLG00000025087
rosa_multiflora Rmu_sc0006944.1_g000001
rosa_roxburghii Rroxscaffold_6G00420230
rosa_rugosa Rorug03G0035800.1 Rorug03G0036200
rosa_samantha Rh3AG093800 Rh3BG096700 Rh3CG097900 Rh3DG097400
rosa_wichuraiana Rw3G008070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 559
AccB1I GGYRCC 1 cut(s) 559
AccB7I CCANNNNNTGG 1 cut(s) 54
AccBSI CCGCTC 2 cut(s) 232, 580
AciI CCGC 7 cut(s) 77, 92, 117, 144, 178, 230, 580
AclWI GGATC 1 cut(s) 489
AcoI YGGCCR 2 cut(s) 145, 474
AcuI CTGAAG 1 cut(s) 92
AcyI GRCGYC 1 cut(s) 65
AfaI GTAC 2 cut(s) 452, 561
AfeI AGCGCT 1 cut(s) 510
AfiI CCNNNNNNNGG 2 cut(s) 54, 623
AgsI TTSAA 2 cut(s) 269, 653
AluBI AGCT 3 cut(s) 190, 340, 394
AluI AGCT 3 cut(s) 190, 340, 394
Alw21I GWGCWC 1 cut(s) 192
Alw26I GTCTC 4 cut(s) 72, 218, 310, 496
AlwI GGATC 1 cut(s) 489
Ama87I CYCGRG 3 cut(s) 95, 208, 423
Aor51HI AGCGCT 1 cut(s) 510
AoxI GGCC 5 cut(s) 145, 244, 347, 379, 474
ApeKI GCWGC 1 cut(s) 82
Asp718I GGTACC 1 cut(s) 559
AspLEI GCGC 3 cut(s) 82, 162, 511
AsuHPI GGTGA 2 cut(s) 97, 271
AvaI CYCGRG 3 cut(s) 95, 208, 423
BalI TGGCCA 1 cut(s) 476
BanI GGYRCC 1 cut(s) 559
BanII GRGCYC 1 cut(s) 192
BauI CACGAG 2 cut(s) 259, 656
Bbv12I GWGCWC 1 cut(s) 192
BbvCI CCTCAGC 1 cut(s) 164
BbvI GCAGC 1 cut(s) 69
BccI CCATC 3 cut(s) 48, 156, 290
BceAI ACGGC 1 cut(s) 42
BciVI GTATCC 1 cut(s) 509
BclI TGATCA 1 cut(s) 465
BcoDI GTCTC 4 cut(s) 72, 218, 310, 496
BfoI RGCGCY 2 cut(s) 83, 512
BfuI GTATCC 1 cut(s) 509
BisI GCNGC 3 cut(s) 78, 83, 145
BlsI GCNGC 3 cut(s) 79, 84, 146
BmeT110I CYCGRG 3 cut(s) 95, 208, 423
BmiI GGNNCC 2 cut(s) 155, 561
BmsI GCATC 1 cut(s) 420
Bpu10I CCTNAGC 3 cut(s) 164, 186, 336
Bsa29I ATCGAT 1 cut(s) 484
BsaAI YACGTR 1 cut(s) 454
BsaHI GRCGYC 1 cut(s) 65
BsaI GGTCTC 1 cut(s) 218
BsaJI CCNNGG 4 cut(s) 241, 477, 617, 639
BsaWI WCCGGW 1 cut(s) 32
BsaXI ACNNNNNCTCC 2 cut(s) 174, 204
Bsc4I CCNNNNNNNGG 2 cut(s) 54, 623
Bse1I ACTGG 1 cut(s) 477
BseCI ATCGAT 1 cut(s) 484
BseDI CCNNGG 4 cut(s) 241, 477, 617, 639
BseLI CCNNNNNNNGG 2 cut(s) 54, 623
BseMII CTCAG 3 cut(s) 177, 178, 404
BseNI ACTGG 1 cut(s) 477
BseRI GAGGAG 1 cut(s) 514
BseXI GCAGC 1 cut(s) 69
BseYI CCCAGC 1 cut(s) 511
BshFI GGCC 5 cut(s) 147, 246, 349, 381, 476
BshNI GGYRCC 1 cut(s) 559
BshVI ATCGAT 1 cut(s) 484
BsiHKAI GWGCWC 1 cut(s) 192
BsiHKCI CYCGRG 3 cut(s) 95, 208, 423
BsiSI CCGG 1 cut(s) 33
BslFI GGGAC 2 cut(s) 223, 641
BslI CCNNNNNNNGG 2 cut(s) 54, 623
BsmAI GTCTC 4 cut(s) 72, 218, 310, 496
BsmBI CGTCTC 1 cut(s) 72
BsmFI GGGAC 2 cut(s) 223, 641
BsnI GGCC 5 cut(s) 147, 246, 349, 381, 476
Bso31I GGTCTC 1 cut(s) 218
BsoBI CYCGRG 3 cut(s) 95, 208, 423
Bsp1286I GDGCHC 1 cut(s) 192
Bsp143I GATC 3 cut(s) 465, 481, 485
Bsp19I CCATGG 1 cut(s) 639
BspACI CCGC 7 cut(s) 77, 92, 117, 144, 178, 230, 580
BspANI GGCC 5 cut(s) 147, 246, 349, 381, 476
BspCNI CTCAG 3 cut(s) 177, 178, 403
BspDI ATCGAT 1 cut(s) 484
BspLI GGNNCC 2 cut(s) 155, 561
BspPI GGATC 1 cut(s) 489
BspT107I GGYRCC 1 cut(s) 559
BspTNI GGTCTC 1 cut(s) 218
BsrBI CCGCTC 2 cut(s) 232, 580
BsrI ACTGG 1 cut(s) 477
BssECI CCNNGG 4 cut(s) 241, 477, 617, 639
BssMI GATC 3 cut(s) 465, 481, 485
BssNI GRCGYC 1 cut(s) 65
BssSI CACGAG 2 cut(s) 259, 656
BssT1I CCWWGG 3 cut(s) 477, 617, 639
Bst2BI CACGAG 2 cut(s) 259, 656
Bst4CI ACNGT 1 cut(s) 109
BstACI GRCGYC 1 cut(s) 65
BstBAI YACGTR 1 cut(s) 454
BstC8I GCNNGC 1 cut(s) 158
BstDEI CTNAG 4 cut(s) 164, 186, 336, 390
BstDSI CCRYGG 1 cut(s) 639
BstH2I RGCGCY 2 cut(s) 83, 512
BstHHI GCGC 3 cut(s) 82, 162, 511
BstKTI GATC 3 cut(s) 468, 484, 488
BstMAI GTCTC 4 cut(s) 72, 218, 310, 496
BstMBI GATC 3 cut(s) 465, 481, 485
BstMWI GCNNNNNNNGC 6 cut(s) 57, 91, 153, 346, 551, 641
BstV1I GCAGC 1 cut(s) 69
Bsu15I ATCGAT 1 cut(s) 484
BsuI GTATCC 1 cut(s) 509
BsuRI GGCC 5 cut(s) 147, 246, 349, 381, 476
BsuTUI ATCGAT 1 cut(s) 484
BtgI CCRYGG 1 cut(s) 639
BtsIMutI CAGTG 1 cut(s) 470
Cac8I GCNNGC 1 cut(s) 158
CfoI GCGC 3 cut(s) 82, 162, 511
ClaI ATCGAT 1 cut(s) 484
CseI GACGC 3 cut(s) 54, 157, 417
Csp6I GTAC 2 cut(s) 451, 560
CspCI CAANNNNNGTGG 2 cut(s) 247, 282
CviAII CATG 5 cut(s) 61, 313, 320, 542, 640
CviQI GTAC 2 cut(s) 451, 560
DdeI CTNAG 4 cut(s) 164, 186, 336, 390
DpnI GATC 3 cut(s) 467, 483, 487
DpnII GATC 3 cut(s) 465, 481, 485
EaeI YGGCCR 2 cut(s) 145, 474
Ecl136II GAGCTC 1 cut(s) 190
Eco130I CCWWGG 3 cut(s) 477, 617, 639
Eco147I AGGCCT 2 cut(s) 349, 381
Eco24I GRGCYC 1 cut(s) 192
Eco31I GGTCTC 1 cut(s) 218
Eco47III AGCGCT 1 cut(s) 510
Eco53kI GAGCTC 1 cut(s) 190
Eco57I CTGAAG 1 cut(s) 92
Eco88I CYCGRG 3 cut(s) 95, 208, 423
EcoICRI GAGCTC 1 cut(s) 190
EcoT14I CCWWGG 3 cut(s) 477, 617, 639
EcoT38I GRGCYC 1 cut(s) 192
ErhI CCWWGG 3 cut(s) 477, 617, 639
Esp3I CGTCTC 1 cut(s) 72
FaeI CATG 5 cut(s) 64, 316, 323, 545, 643
FalI AAGNNNNNCTT 2 cut(s) 357, 389
FaqI GGGAC 2 cut(s) 223, 641
FatI CATG 5 cut(s) 60, 312, 319, 541, 639
FauI CCCGC 2 cut(s) 99, 237
FbaI TGATCA 1 cut(s) 465
Fnu4HI GCNGC 3 cut(s) 78, 83, 145
FriOI GRGCYC 1 cut(s) 192
Fsp4HI GCNGC 3 cut(s) 78, 83, 145
GlaI GCGC 3 cut(s) 81, 161, 510
GluI GCNGC 3 cut(s) 78, 83, 145
GsaI CCCAGC 1 cut(s) 515
HaeII RGCGCY 2 cut(s) 83, 512
HaeIII GGCC 5 cut(s) 147, 246, 349, 381, 476
HapII CCGG 1 cut(s) 33
HgaI GACGC 3 cut(s) 54, 157, 417
HhaI GCGC 3 cut(s) 82, 162, 511
Hin1I GRCGYC 1 cut(s) 65
Hin1II CATG 5 cut(s) 64, 316, 323, 545, 643
Hin6I GCGC 3 cut(s) 80, 160, 509
HinP1I GCGC 3 cut(s) 80, 160, 509
HinfI GANTC 3 cut(s) 174, 254, 600
HpaII CCGG 1 cut(s) 33
HphI GGTGA 2 cut(s) 97, 271
Hpy166II GTNNAC 3 cut(s) 105, 258, 457
Hpy188I TCNGA 5 cut(s) 72, 153, 173, 253, 608
Hpy188III TCNNGA 1 cut(s) 353
Hpy8I GTNNAC 3 cut(s) 105, 258, 457
Hpy99I CGWCG 2 cut(s) 10, 173
HpyAV CCTTC 1 cut(s) 67
HpyCH4III ACNGT 1 cut(s) 109
HpyCH4IV ACGT 2 cut(s) 140, 453
HpyCH4V TGCA 2 cut(s) 554, 635
HpyF10VI GCNNNNNNNGC 6 cut(s) 57, 91, 153, 346, 551, 641
HpyF3I CTNAG 4 cut(s) 164, 186, 336, 390
HpySE526I ACGT 2 cut(s) 140, 453
Hsp92I GRCGYC 1 cut(s) 65
Hsp92II CATG 5 cut(s) 64, 316, 323, 545, 643
HspAI GCGC 3 cut(s) 80, 160, 509
KpnI GGTACC 1 cut(s) 563
Ksp22I TGATCA 1 cut(s) 465
Kzo9I GATC 3 cut(s) 465, 481, 485
LmnI GCTCC 6 cut(s) 57, 153, 195, 237, 399, 577
Lsp1109I GCAGC 1 cut(s) 69
LweI GCATC 1 cut(s) 420
MaeII ACGT 2 cut(s) 140, 453
MalI GATC 3 cut(s) 467, 483, 487
MbiI CCGCTC 2 cut(s) 232, 580
MboI GATC 3 cut(s) 465, 481, 485
MboII GAAGA 5 cut(s) 114, 117, 120, 123, 365
MhlI GDGCHC 1 cut(s) 192
MlsI TGGCCA 1 cut(s) 476
MluCI AATT 1 cut(s) 194
MluNI TGGCCA 1 cut(s) 476
MlyI GAGTC 2 cut(s) 168, 263
MmeI TCCRAC 1 cut(s) 151
Mox20I TGGCCA 1 cut(s) 476
MscI TGGCCA 1 cut(s) 476
MseI TTAA 1 cut(s) 399
Msp20I TGGCCA 1 cut(s) 476
MspI CCGG 1 cut(s) 33
MwoI GCNNNNNNNGC 6 cut(s) 57, 91, 153, 346, 551, 641
NcoI CCATGG 1 cut(s) 639
NdeII GATC 3 cut(s) 465, 481, 485
NlaIII CATG 5 cut(s) 64, 316, 323, 545, 643
NlaIV GGNNCC 2 cut(s) 155, 561
NmeAIII GCCGAG 1 cut(s) 222
PaeR7I CTCGAG 1 cut(s) 423
PceI AGGCCT 2 cut(s) 349, 381
PcsI WCGNNNNNNNCGW 1 cut(s) 249
PfeI GAWTC 1 cut(s) 600
PflMI CCANNNNNTGG 1 cut(s) 54
PkrI GCNGC 3 cut(s) 79, 84, 146
PleI GAGTC 2 cut(s) 168, 262
PpsI GAGTC 2 cut(s) 168, 262
Ppu21I YACGTR 1 cut(s) 454
Psp124BI GAGCTC 1 cut(s) 192
PspFI CCCAGC 1 cut(s) 511
PspN4I GGNNCC 2 cut(s) 155, 561
PspXI VCTCGAGB 1 cut(s) 423
RsaI GTAC 2 cut(s) 452, 561
RsaNI GTAC 2 cut(s) 451, 560
SacI GAGCTC 1 cut(s) 192
SaqAI TTAA 1 cut(s) 399
SatI GCNGC 3 cut(s) 78, 83, 145
Sau3AI GATC 3 cut(s) 465, 481, 485
SchI GAGTC 2 cut(s) 168, 263
SduI GDGCHC 1 cut(s) 192
SetI ASST 9 cut(s) 104, 143, 187, 192, 342, 396, 456, 561, 565
SfaNI GCATC 1 cut(s) 420
Sfr274I CTCGAG 1 cut(s) 423
SlaI CTCGAG 1 cut(s) 423
SmlI CTYRAG 1 cut(s) 423
SmoI CTYRAG 1 cut(s) 423
Sse9I AATT 1 cut(s) 194
SseBI AGGCCT 2 cut(s) 349, 381
SsiI CCGC 7 cut(s) 77, 92, 117, 144, 178, 230, 580
SstI GAGCTC 1 cut(s) 192
StuI AGGCCT 2 cut(s) 349, 381
StyI CCWWGG 3 cut(s) 477, 617, 639
TaaI ACNGT 1 cut(s) 109
TaiI ACGT 2 cut(s) 143, 456
TaqI TCGA 3 cut(s) 376, 424, 484
TasI AATT 1 cut(s) 194
TauI GCSGC 2 cut(s) 80, 147
TfiI GAWTC 1 cut(s) 600
Tru1I TTAA 1 cut(s) 399
Tru9I TTAA 1 cut(s) 399
TscAI CASTG 1 cut(s) 477
TseI GCWGC 1 cut(s) 82
TspGWI ACGGA 1 cut(s) 420
TspRI CASTG 1 cut(s) 477
Van91I CCANNNNNTGG 1 cut(s) 54
XhoI CTCGAG 1 cut(s) 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.