MD12G1197200.v1.1

Regulatory photoreceptor which exists in two forms that are reversibly interconvertible by light the Pr form that absorbs maximally in the red region of the spectrum and the Pfr form that absorbs maximally in the far-red region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0007386
Physical Location & Seq
Forward (+)
27846541 .. 27852368
5828 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1197200.v1.1.491

Sequence Viewer

Length: 3405 bp
ATGCAGTCGTGTAAAAGTTTTGTTTGTGCGAGAATGTCTTCTTCTAGACCTAGTAACTCATCAAGCAATCCAGGGCGGTCTAGACACAGCTCTAGAATCATTGCTCAGACAACTGTTGATGCAAAGATTCATGCCGATTTTGAGGAATCGGGTGGTTCCTTTGACTACTCGAACTCAGTCCGTGTTGGCAGTTCAGTTGGTGGAGATCAACAACCTAGGTCCGACAAAGTGACCACTGCTTATCTCCATAACATACAGAAAGGCAAGACGATCCAACCGTTTGGATGCTTGCTAGCCTTGGATGAGAAGACTTTCAAGGTCATTGCATACAGTGCGAATGCCCCTGAAATGCTGACGACGGTCAGTCATGCAGTCCCAAGCGTCGGGGAATCCCCTGTTCTTGGCATAGGAACAGATATAAGAACAATTTTTACCGCCCCTAGTGCCTCTGCACTGCATAAGGCCCTTGGATTTGGAGAGGTTTCTCTCTTAAATCCCATCTTAGTTCATTGCAAGACCTCTGGCAAGCCATTCTATGCAATAACTCATCGAGTAACGGGCAGTTTGATCATTGACTTTGAGCCGGTGAAGCCATATGAAGTGCCTATGACTGCTGCTGGGGCGCTGCAATCTTACAAGCTTGCTGCCAAAGCAATTGCCAGATTGCAGTCTTTGCCTAGCGGGAGCATGGAGAGGCTTTGTGATACAATGGTCCAAGAGGTTTTTGAACTCACCGGTTATGACAGGGTGATGACTTATAAATTTCATGATGATGATCACGGAGAAGTGGTCTCTGAGCTTACGAAGCCTGGCCTGGAGCCATATCTAGGGTTGCATTATCCATCCACTGACATACCTCAGGCTGCACGGTTCCTATTTATGAAGAATAAGGTCCGCATGATTGTCGATTGTGGTGCGAAACTTGTGAAGGTGTTTCAAGATGAGAAGCTTCCGCTGGATCTCACATTGTGCGGTTCAACCCTGAGAGCCCCACACAGTTGCCATTTAGAATATATGAAGAATATGAATTCCATCGCATCTCTTGTTATGGCAGTAGTTGTTAATGAGGGGGATGACGAGGTGAGAGATCCTGATTCTGTTCCGCCTCAAAAAAGAAAGAGACTTTGGGGTTTGGTAGTATGCCATAATACTAGTCCAAGATTTGTTGCTTTCCCTCTCAGGTATGCCTGTGAGTTTTTAACTCAAGTGTTTGCAATACATGTCAATAAGGAGATAGAGATGGAAGATCAGATGGTTGAGAAGAACATCCTGCGCACCCAAACGCTGTTGTGTGATATGTTGTTACGAGATGCACCCCTAGGTATTATGTCACAGAGTCCTAATGTTATGGATCTAGTGAAATGTGATGGAGCTGCCCTATTGTACAAGAACAAGGTATGGAGACTTGGAATGACTCCGAGTGACTTTCAGCTTCATGACATAGCTTCATGGCTCTCCGAGTACCATATGGATTCTACAGGTTTGAGTACAGATAGCTTGTATGATGCAGGATTCCCTGGGGCTCTTGCTCTTGGTGATGTAGTATGTGGAATGGCAGCTGTGTGGATAACTTCCAAGGACGTGCTCTTTTGGCTTCGATCCCACACTGCTGCAGAAATTCGATGGGGTGGTGCAAAACATGAAACCGGTGAGAAAGATGATGGTTCTAAAATGCACCCTAGATCATCATTCAAGGCTTTCCTTGAAGTTGTCAAGACGAGGAGCTTGCCTTGGAAGGACTATGAAATGGATGCAATTCATTCTTTGCAGCTTATTCTGAGGAATGCTTTCAAAGATGTTGAAACTACCTTGGAAGCAAACACCAAAGCAATCAATGTAAGGCTTAGTGACCTCAAACTTGAAGGGATGGAAGAACTGGAAGCTGTGACAAGTGAGATGGTCCGTTTAATTGAAACAGCCACGGTTCCAATTTTGGCTGTTGATGTTGATGGGCTGGTTAATGGGTGGAATACAAAAATTGATGAGTTAACTGGTCTTCCTGTTGATGAAGCAATCGGAAAGCATTTGCTCACACTCGTGGAGGATTCTTCGACTAGCGTGGTGAAGAAAATGTTGGACTCAGCATTGCATGGCAAAGAGGAACAAAACATCCAATTCGAGATTAAAACACATGGTTCTAGGGCTGATCTTGGTCCCATCAGCTTAGTTGTGAATGCTTGTGCAAGTAGAGACATTCGGGAAAATGTTGTGGGTGTGTGTTTTGTGGCACAGGATATCACTGGTCAGAAGAATGTGATGGACAAATTCACCCGGATCGAGGGTGATTACAAAGCGATTGTACAAAACCCTAGCCCCTTGATTCCCCCAATATTTGGTACAGATGAATTTGGCTGGTGTTCTGAGTGGAATTCAGCAATGACAAAGTTAACAGGGTGGAAACGAGAGGAGGTAATGGATAAAATGCTTCTGGGAGAGGTTTTTGGGATCAACATGGCATGTTGTCCTCTCAAGAGCCAAGAATCTTTCGTAAATCTTGGTGTTGTACTTAACCATGCCATGACAGGTCAGGTGTCCGAAAAGCTTCCCTTTGGTTTCTGTGCTCGTAGTGGAAAACACATAGAATGCCTGCTGTGCGTGAGTAAGAAATTGAACAGTGAGGGCTCAGTCACTGGGGTCTTTTGCTTCTTGCAGCTTGCTAGCCCAGAGCTGCAACAAGCACTTCATGTCCAACGCTTATCAGAACAAACTGCTGTGAAGAGATTGAAGGAATTGTCTTATATTAGAAGGCAGATCCGGAATCCTTTAGCCGGTATAATATTTTCTCGGAAAATGATGGAGGATACTGAGTTGGGAGCAGAACAACAACGGCTTCTGCATACGAGCGCCCAGTGCCAGCACCAGCTCAACAAAATACTGGATGATTCAGATCTTGATACCATCATTGATGGCTACCTGGACCTGGAGATGGTCGAGTTTACATTGCATGAAGTACTGAATGCCGCTGTCAGTCAAGTCATGATTAAGAGCAATGCCAAGAGCGTCCGCATTGTCCACAATGCGGCGGAAGATATCATGACTGAGACGTTATATGGGGATAGTCTTAGGCTTCAACAAGTCATAGCTGATTTCTTAGCCGTATCAGTTAGTTTTATGCCAACTGGAGGCCAGCTTACTATCGAAGCTAATTTGACCAAAGATCGGTTAGGACAATCCGTTCAGCTCGTGCATTTGGAGCTCAGGATCACACACGCCGGTGGTGGGATTCCAGAAGGATTGCTGAACCAGATGTTTGGAAACAACGGAGACATATCTGAGGAGGGCATTAGCCTGCTCATCAGCAGTAAACTAGTGAAGCTCATGAATGGAGACGTACGGTATCTTAGGGAAGAAGGTAGGTCAACCTTCATGATATCCGTAGAACTCGCTGCGGCACACAAGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0004672 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0006417 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007275 GO:0007602 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009581 GO:0009582 GO:0009583 GO:0009585 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009637 GO:0009638 GO:0009639 GO:0009640 GO:0009642 GO:0009791 GO:0009881 GO:0009883 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010017 GO:0010018 GO:0010114 GO:0010161 GO:0010201 GO:0010203 GO:0010218 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0016301 GO:0016310 GO:0016604 GO:0016740 GO:0016772 GO:0016773 GO:0017148 GO:0019222 GO:0019538 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031516 GO:0031974 GO:0031981 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0034248 GO:0034249 GO:0036211 GO:0038023 GO:0042221 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046685 GO:0048519 GO:0048523 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051606 GO:0051716 GO:0055122 GO:0060089 GO:0060255 GO:0065007 GO:0070013 GO:0071214 GO:0071478 GO:0071482 GO:0071489 GO:0071490 GO:0071491 GO:0071704 GO:0080090 GO:0097159 GO:0104004 GO:0140096 GO:1901363 GO:1901564 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
ko04712 map04712
Pfam Domains
Protein Families
GAF HATPase_c HisKA PAS PAS_2 PHY

Protein Analysis

1135

Amino Acids

125.25

Weight (kDa)

5.84

Isoelectric Point (pI)

47.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS_2 PF08446 80 - 196 5.5e-35 PAS fold
GAF PF01590 230 - 412 4.9e-15 GAF domain
PHY PF00360 425 - 598 4.3e-54 Phytochrome region
PAS PF00989 632 - 746 2.6e-21 PAS fold
PAS_4 PF08448 640 - 750 6.8e-06 PAS fold
PAS PF00989 762 - 884 8.5e-22 PAS fold
HisKA PF00512 907 - 964 8.7e-10 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 1018 - 1127 2.4e-11 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 759
Acc16I TGCGCA 1 cut(s) 1274
AccB7I CCANNNNNTGG 1 cut(s) 2333
AccIII TCCGGA 1 cut(s) 2754
AclWI GGATC 9 cut(s) 265, 966, 1082, 1359, 1593, 2282, 2453, 2746, 3209
AcsI RAATTY 6 cut(s) 761, 1027, 1617, 2264, 2345, 2368
AdeI CACNNNGTG 1 cut(s) 969
AfaI GTAC 8 cut(s) 1385, 1463, 1489, 2301, 2338, 2505, 2952, 3333
AflIII ACRYGT 1 cut(s) 1219
AgeI ACCGGT 2 cut(s) 734, 1646
AhdI GACNNNNNGTC 1 cut(s) 363
AhlI ACTAGT 2 cut(s) 1151, 3307
AjiI CACGTC 1 cut(s) 1582
AjnI CCWGG 6 cut(s) 70, 808, 813, 1516, 2913, 2919
AjuI GAANNNNNNNTTGG 4 cut(s) 1108, 1140, 2057, 2089
AleI CACNNNNGTG 2 cut(s) 2036, 3213
AloI GAACNNNNNNTCC 4 cut(s) 2094, 2126, 3159, 3191
Alw21I GWGCWC 3 cut(s) 1587, 2563, 3198
Alw26I GTCTC 7 cut(s) 796, 1114, 1396, 2184, 3035, 3258, 3321
AlwI GGATC 9 cut(s) 265, 966, 1082, 1359, 1593, 2282, 2453, 2746, 3209
AlwNI CAGNNNCTG 1 cut(s) 2630
Aor13HI TCCGGA 1 cut(s) 2754
AoxI GGCC 3 cut(s) 462, 811, 3124
ApoI RAATTY 6 cut(s) 761, 1027, 1617, 2264, 2345, 2368
AsiGI ACCGGT 2 cut(s) 734, 1646
Asp700I GAANNNNTTC 4 cut(s) 37, 311, 1787, 2541
AspA2I CCTAGG 2 cut(s) 215, 1318
AspLEI GCGC 3 cut(s) 625, 1275, 2846
AspS9I GGNCC 7 cut(s) 219, 463, 712, 892, 1900, 2153, 2917
AsuC2I CCSGG 1 cut(s) 2272
AsuHPI GGTGA 9 cut(s) 598, 724, 760, 1093, 1547, 1661, 2074, 2260, 2294
AsuNHI GCTAGC 2 cut(s) 292, 2657
AvaII GGWCC 6 cut(s) 219, 712, 892, 1900, 2153, 2917
AvrII CCTAGG 2 cut(s) 215, 1318
AxyI CCTNAGG 1 cut(s) 858
BaeI ACNNNNGTAYC 2 cut(s) 696, 729
BanII GRGCYC 4 cut(s) 991, 1525, 2624, 3198
BauI CACGAG 2 cut(s) 2036, 3182
BbsI GAAGAC 3 cut(s) 30, 314, 1988
Bbv12I GWGCWC 3 cut(s) 1587, 2563, 3198
BceAI ACGGC 2 cut(s) 2843, 3080
BcgI CGANNNNNNTGC 6 cut(s) 886, 896, 920, 930, 1708, 1742
BciT130I CCWGG 6 cut(s) 72, 810, 815, 1518, 2915, 2921
BciVI GTATCC 1 cut(s) 2794
BclI TGATCA 2 cut(s) 567, 775
BcnI CCSGG 1 cut(s) 2272
BcoDI GTCTC 7 cut(s) 796, 1114, 1396, 2184, 3035, 3258, 3321
BcuI ACTAGT 2 cut(s) 1151, 3307
BfmI CTRYAG 2 cut(s) 1476, 1611
BfoI RGCGCY 2 cut(s) 626, 2847
BfuI GTATCC 1 cut(s) 2794
BglII AGATCT 1 cut(s) 2887
BlnI CCTAGG 2 cut(s) 215, 1318
BmcAI AGTACT 1 cut(s) 2952
Bme1390I CCNGG 7 cut(s) 72, 810, 815, 1518, 2272, 2915, 2921
Bme18I GGWCC 6 cut(s) 219, 712, 892, 1900, 2153, 2917
BmeRI GACNNNNNGTC 1 cut(s) 363
BmgBI CACGTC 1 cut(s) 1582
BmgT120I GGNCC 7 cut(s) 219, 463, 712, 892, 1900, 2153, 2917
BmiI GGNNCC 5 cut(s) 157, 819, 872, 1926, 2155
BmrFI CCNGG 7 cut(s) 72, 810, 815, 1518, 2272, 2915, 2921
BmrI ACTGGG 2 cut(s) 2640, 2842
BmsI GCATC 6 cut(s) 109, 275, 1046, 1300, 1495, 1741
BmtI GCTAGC 2 cut(s) 296, 2661
BmuI ACTGGG 2 cut(s) 2640, 2842
BoxI GACNNNNGTC 1 cut(s) 359
BpiI GAAGAC 3 cut(s) 30, 314, 1988
BpmI CTGGAG 3 cut(s) 836, 2942, 3141
Bpu10I CCTNAGC 1 cut(s) 3197
BpuEI CTTGAG 2 cut(s) 1188, 2453
BpuMI CCSGG 1 cut(s) 2272
BsaBI GATNNNNATC 2 cut(s) 774, 2886
BsaI GGTCTC 1 cut(s) 796
BsaWI WCCGGW 3 cut(s) 734, 1646, 2754
BsaXI ACNNNNNCTCC 2 cut(s) 774, 804
Bse118I RCCGGY 5 cut(s) 583, 734, 1646, 2768, 3212
Bse1I ACTGG 7 cut(s) 1881, 1996, 2245, 2635, 2848, 2880, 3124
Bse21I CCTNAGG 1 cut(s) 858
Bse3DI GCAATG 7 cut(s) 99, 321, 508, 2084, 2382, 2939, 2995
Bse8I GATNNNNATC 2 cut(s) 774, 2886
BseAI TCCGGA 1 cut(s) 2754
BseBI CCWGG 6 cut(s) 72, 810, 815, 1518, 2915, 2921
BseGI GGATG 9 cut(s) 290, 307, 842, 1078, 1266, 1756, 1872, 2109, 2884
BseJI GATNNNNATC 2 cut(s) 774, 2886
BseMI GCAATG 7 cut(s) 99, 321, 508, 2084, 2382, 2939, 2995
BseNI ACTGG 7 cut(s) 1881, 1996, 2245, 2635, 2848, 2880, 3124
BseRI GAGGAG 3 cut(s) 1735, 2420, 3290
BseYI CCCAGC 1 cut(s) 617
BsgI GTGCAG 2 cut(s) 435, 849
BshFI GGCC 3 cut(s) 464, 813, 3126
BshTI ACCGGT 2 cut(s) 734, 1646
BsiHKAI GWGCWC 3 cut(s) 1587, 2563, 3198
BsiSI CCGG 7 cut(s) 584, 735, 1647, 2272, 2755, 2769, 3213
BsiWI CGTACG 1 cut(s) 3331
BslFI GGGAC 2 cut(s) 359, 2139
BsmAI GTCTC 7 cut(s) 796, 1114, 1396, 2184, 3035, 3258, 3321
BsmBI CGTCTC 2 cut(s) 3035, 3321
BsmFI GGGAC 2 cut(s) 359, 2139
BsmI GAATGC 5 cut(s) 343, 1789, 2179, 2588, 2962
BsnI GGCC 3 cut(s) 464, 813, 3126
Bso31I GGTCTC 1 cut(s) 796
Bsp1286I GDGCHC 6 cut(s) 991, 1525, 1587, 2563, 2624, 3198
Bsp13I TCCGGA 1 cut(s) 2754
Bsp1407I TGTACA 2 cut(s) 1383, 2299
BspANI GGCC 3 cut(s) 464, 813, 3126
BspEI TCCGGA 1 cut(s) 2754
BspHI TCATGA 6 cut(s) 766, 1435, 2976, 3033, 3318, 3366
BspLI GGNNCC 5 cut(s) 157, 819, 872, 1926, 2155
BspMAI CTGCAG 1 cut(s) 1615
BspOI GCTAGC 2 cut(s) 296, 2661
BspPI GGATC 9 cut(s) 265, 966, 1082, 1359, 1593, 2282, 2453, 2746, 3209
BspTNI GGTCTC 1 cut(s) 796
BsrDI GCAATG 7 cut(s) 99, 321, 508, 2084, 2382, 2939, 2995
BsrFI RCCGGY 5 cut(s) 583, 734, 1646, 2768, 3212
BsrGI TGTACA 2 cut(s) 1383, 2299
BsrI ACTGG 7 cut(s) 1881, 1996, 2245, 2635, 2848, 2880, 3124
BssAI RCCGGY 5 cut(s) 583, 734, 1646, 2768, 3212
BssSI CACGAG 2 cut(s) 2036, 3182
BssT1I CCWWGG 7 cut(s) 215, 297, 466, 1318, 1575, 1730, 1809
Bst2BI CACGAG 2 cut(s) 2036, 3182
Bst2UI CCWGG 6 cut(s) 72, 810, 815, 1518, 2915, 2921
Bst4CI ACNGT 9 cut(s) 115, 279, 332, 361, 870, 998, 1924, 2615, 3336
Bst6I CTCTTC 1 cut(s) 2711
BstAPI GCANNNNNTGC 2 cut(s) 332, 673
BstAUI TGTACA 2 cut(s) 1383, 2299
BstDSI CCRYGG 1 cut(s) 1920
BstF5I GGATG 9 cut(s) 290, 307, 842, 1078, 1266, 1756, 1872, 2109, 2884
BstH2I RGCGCY 2 cut(s) 626, 2847
BstHHI GCGC 3 cut(s) 625, 1275, 2846
BstMAI GTCTC 7 cut(s) 796, 1114, 1396, 2184, 3035, 3258, 3321
BstNI CCWGG 6 cut(s) 72, 810, 815, 1518, 2915, 2921
BstNSI RCATGY 2 cut(s) 1223, 2460
BstPAI GACNNNNGTC 1 cut(s) 359
BstSCI CCNGG 7 cut(s) 70, 808, 813, 1516, 2270, 2913, 2919
BstSFI CTRYAG 2 cut(s) 1476, 1611
BstV2I GAAGAC 3 cut(s) 30, 314, 1988
BstX2I RGATCY 5 cut(s) 958, 1087, 1351, 2751, 2887
BstXI CCANNNNNNTGG 2 cut(s) 281, 3251
BstYI RGATCY 5 cut(s) 958, 1087, 1351, 2751, 2887
Bsu36I CCTNAGG 1 cut(s) 858
BsuI GTATCC 1 cut(s) 2794
BsuRI GGCC 3 cut(s) 464, 813, 3126
BtgI CCRYGG 1 cut(s) 1920
BtgZI GCGATG 1 cut(s) 1018
BtrI CACGTC 1 cut(s) 1582
BtsCI GGATG 9 cut(s) 290, 307, 842, 1078, 1266, 1756, 1872, 2109, 2884
BtsI GCAGTG 3 cut(s) 234, 452, 1605
BtsIMutI CAGTG 9 cut(s) 234, 337, 452, 846, 1605, 2238, 2620, 2628, 2855
CaiI CAGNNNCTG 1 cut(s) 2630
CciI TCATGA 6 cut(s) 766, 1435, 2976, 3033, 3318, 3366
CfoI GCGC 3 cut(s) 625, 1275, 2846
Cfr10I RCCGGY 5 cut(s) 583, 734, 1646, 2768, 3212
Cfr13I GGNCC 7 cut(s) 219, 463, 712, 892, 1900, 2153, 2917
CseI GACGC 2 cut(s) 370, 2989
Csp6I GTAC 8 cut(s) 1384, 1462, 1488, 2300, 2337, 2504, 2951, 3332
CspAI ACCGGT 2 cut(s) 734, 1646
CviQI GTAC 8 cut(s) 1384, 1462, 1488, 2300, 2337, 2504, 2951, 3332
DraIII CACNNNGTG 1 cut(s) 969
DriI GACNNNNNGTC 1 cut(s) 363
Eam1104I CTCTTC 1 cut(s) 2711
Eam1105I GACNNNNNGTC 1 cut(s) 363
EarI CTCTTC 1 cut(s) 2711
EciI GGCGGA 2 cut(s) 1092, 3038
Ecl136II GAGCTC 1 cut(s) 3196
Eco130I CCWWGG 7 cut(s) 215, 297, 466, 1318, 1575, 1730, 1809
Eco24I GRGCYC 4 cut(s) 991, 1525, 2624, 3198
Eco31I GGTCTC 1 cut(s) 796
Eco32I GATATC 3 cut(s) 2236, 3031, 3372
Eco47I GGWCC 6 cut(s) 219, 712, 892, 1900, 2153, 2917
Eco53kI GAGCTC 1 cut(s) 3196
Eco81I CCTNAGG 1 cut(s) 858
EcoICRI GAGCTC 1 cut(s) 3196
EcoO109I RGGNCCY 1 cut(s) 463
EcoRI GAATTC 2 cut(s) 1027, 2368
EcoRII CCWGG 6 cut(s) 70, 808, 813, 1516, 2913, 2919
EcoRV GATATC 3 cut(s) 2236, 3031, 3372
EcoT14I CCWWGG 7 cut(s) 215, 297, 466, 1318, 1575, 1730, 1809
EcoT38I GRGCYC 4 cut(s) 991, 1525, 2624, 3198
ErhI CCWWGG 7 cut(s) 215, 297, 466, 1318, 1575, 1730, 1809
Esp3I CGTCTC 2 cut(s) 3035, 3321
FalI AAGNNNNNCTT 2 cut(s) 2531, 2563
FaqI GGGAC 2 cut(s) 359, 2139
FauI CCCGC 1 cut(s) 674
FauNDI CATATG 2 cut(s) 595, 1467
FbaI TGATCA 2 cut(s) 567, 775
FokI GGATG 9 cut(s) 297, 314, 829, 1085, 1253, 1763, 1879, 2096, 2891
FriOI GRGCYC 4 cut(s) 991, 1525, 2624, 3198
FspI TGCGCA 1 cut(s) 1274
GlaI GCGC 3 cut(s) 624, 1274, 2845
GsaI CCCAGC 1 cut(s) 621
GsuI CTGGAG 3 cut(s) 836, 2942, 3141
HaeII RGCGCY 2 cut(s) 626, 2847
HaeIII GGCC 3 cut(s) 464, 813, 3126
HapII CCGG 7 cut(s) 584, 735, 1647, 2272, 2755, 2769, 3213
HgaI GACGC 2 cut(s) 370, 2989
HhaI GCGC 3 cut(s) 625, 1275, 2846
Hin6I GCGC 3 cut(s) 623, 1273, 2844
HinP1I GCGC 3 cut(s) 623, 1273, 2844
HincII GTYRAC 3 cut(s) 1989, 2388, 3360
HindII GTYRAC 3 cut(s) 1989, 2388, 3360
HindIII AAGCTT 3 cut(s) 638, 947, 2540
HpaI GTTAAC 2 cut(s) 1989, 2388
HpaII CCGG 7 cut(s) 584, 735, 1647, 2272, 2755, 2769, 3213
HphI GGTGA 9 cut(s) 598, 724, 760, 1093, 1547, 1661, 2074, 2260, 2294
Hpy166II GTNNAC 6 cut(s) 1989, 2388, 2937, 3013, 3305, 3360
Hpy8I GTNNAC 6 cut(s) 1989, 2388, 2937, 3013, 3305, 3360
Hpy99I CGWCG 2 cut(s) 361, 386
HpyAV CCTTC 8 cut(s) 922, 1729, 1856, 2719, 2739, 3224, 3344, 3373
HpyCH4III ACNGT 9 cut(s) 115, 279, 332, 361, 870, 998, 1924, 2615, 3336
HpyCH4IV ACGT 3 cut(s) 1581, 3044, 3330
HpySE526I ACGT 3 cut(s) 1581, 3044, 3330
HspAI GCGC 3 cut(s) 623, 1273, 2844
Kpn2I TCCGGA 1 cut(s) 2754
Ksp22I TGATCA 2 cut(s) 567, 775
KspAI GTTAAC 2 cut(s) 1989, 2388
LmnI GCTCC 6 cut(s) 684, 817, 1370, 1722, 2813, 3193
LweI GCATC 6 cut(s) 109, 275, 1046, 1300, 1495, 1741
MaeII ACGT 3 cut(s) 1581, 3044, 3330
MaeIII GTNAC 9 cut(s) 53, 229, 553, 1302, 1329, 1421, 1847, 1885, 2626
MfeI CAATTG 1 cut(s) 654
MflI RGATCY 5 cut(s) 958, 1087, 1351, 2751, 2887
MhlI GDGCHC 6 cut(s) 991, 1525, 1587, 2563, 2624, 3198
MlyI GAGTC 3 cut(s) 1345, 1408, 2072
MmeI TCCRAC 4 cut(s) 246, 298, 2055, 2713
MroI TCCGGA 1 cut(s) 2754
MroXI GAANNNNTTC 4 cut(s) 37, 311, 1787, 2541
MslI CAYNNNNRTG 3 cut(s) 771, 2036, 3213
MspA1I CMGCKG 3 cut(s) 955, 1559, 2963
MspI CCGG 7 cut(s) 584, 735, 1647, 2272, 2755, 2769, 3213
MspR9I CCNGG 7 cut(s) 72, 810, 815, 1518, 2272, 2915, 2921
MunI CAATTG 1 cut(s) 654
Mva1269I GAATGC 5 cut(s) 343, 1789, 2179, 2588, 2962
MvaI CCWGG 6 cut(s) 72, 810, 815, 1518, 2915, 2921
NciI CCSGG 1 cut(s) 2272
NdeI CATATG 2 cut(s) 595, 1467
NheI GCTAGC 2 cut(s) 292, 2657
NlaIV GGNNCC 5 cut(s) 157, 819, 872, 1926, 2155
NmuCI GTSAC 6 cut(s) 229, 1329, 1421, 1847, 1885, 2626
NsbI TGCGCA 1 cut(s) 1274
NspI RCATGY 2 cut(s) 1223, 2460
OliI CACNNNNGTG 2 cut(s) 2036, 3213
PagI TCATGA 6 cut(s) 766, 1435, 2976, 3033, 3318, 3366
PasI CCCWGGG 1 cut(s) 1517
PciI ACATGT 1 cut(s) 1219
PcsI WCGNNNNNNNCGW 1 cut(s) 275
PctI GAATGC 5 cut(s) 343, 1789, 2179, 2588, 2962
PdmI GAANNNNTTC 4 cut(s) 37, 311, 1787, 2541
Pfl23II CGTACG 1 cut(s) 3331
PflMI CCANNNNNTGG 1 cut(s) 2333
PinAI ACCGGT 2 cut(s) 734, 1646
PleI GAGTC 3 cut(s) 1344, 1408, 2072
PpsI GAGTC 3 cut(s) 1344, 1408, 2072
PscI ACATGT 1 cut(s) 1219
PshAI GACNNNNGTC 1 cut(s) 359
PsiI TTATAA 1 cut(s) 759
Psp124BI GAGCTC 1 cut(s) 3198
Psp6I CCWGG 6 cut(s) 70, 808, 813, 1516, 2913, 2919
PspFI CCCAGC 1 cut(s) 617
PspGI CCWGG 6 cut(s) 70, 808, 813, 1516, 2913, 2919
PspLI CGTACG 1 cut(s) 3331
PspN4I GGNNCC 5 cut(s) 157, 819, 872, 1926, 2155
PspPI GGNCC 7 cut(s) 219, 463, 712, 892, 1900, 2153, 2917
PsrI GAACNNNNNNTAC 2 cut(s) 415, 447
PstI CTGCAG 1 cut(s) 1615
PstNI CAGNNNCTG 1 cut(s) 2630
PsuI RGATCY 5 cut(s) 958, 1087, 1351, 2751, 2887
PvuII CAGCTG 1 cut(s) 1559
RsaI GTAC 8 cut(s) 1385, 1463, 1489, 2301, 2338, 2505, 2952, 3333
RsaNI GTAC 8 cut(s) 1384, 1462, 1488, 2300, 2337, 2504, 2951, 3332
RseI CAYNNNNRTG 3 cut(s) 771, 2036, 3213
SacI GAGCTC 1 cut(s) 3198
Sau96I GGNCC 7 cut(s) 219, 463, 712, 892, 1900, 2153, 2917
ScaI AGTACT 1 cut(s) 2952
SchI GAGTC 3 cut(s) 1345, 1408, 2072
ScrFI CCNGG 7 cut(s) 72, 810, 815, 1518, 2272, 2915, 2921
SduI GDGCHC 6 cut(s) 991, 1525, 1587, 2563, 2624, 3198
SfaNI GCATC 6 cut(s) 109, 275, 1046, 1300, 1495, 1741
SfcI CTRYAG 2 cut(s) 1476, 1611
SgrAI CRCCGGYG 1 cut(s) 3212
SinI GGWCC 6 cut(s) 219, 712, 892, 1900, 2153, 2917
SmiMI CAYNNNNRTG 3 cut(s) 771, 2036, 3213
SmlI CTYRAG 2 cut(s) 1203, 2468
SmoI CTYRAG 2 cut(s) 1203, 2468
SpeI ACTAGT 2 cut(s) 1151, 3307
SspI AATATT 2 cut(s) 2331, 2778
SstI GAGCTC 1 cut(s) 3198
StyD4I CCNGG 7 cut(s) 70, 808, 813, 1516, 2270, 2913, 2919
StyI CCWWGG 7 cut(s) 215, 297, 466, 1318, 1575, 1730, 1809
TaaI ACNGT 9 cut(s) 115, 279, 332, 361, 870, 998, 1924, 2615, 3336
TaiI ACGT 3 cut(s) 1584, 3047, 3333
TatI WGTACW 5 cut(s) 1383, 1487, 2299, 2503, 2950
TauI GCSGC 3 cut(s) 2963, 3023, 3392
TscAI CASTG 9 cut(s) 241, 337, 459, 853, 1612, 2245, 2620, 2635, 2855
TseFI GTSAC 6 cut(s) 229, 1329, 1421, 1847, 1885, 2626
Tsp45I GTSAC 6 cut(s) 229, 1329, 1421, 1847, 1885, 2626
TspGWI ACGGA 6 cut(s) 170, 795, 1892, 3163, 3276, 3364
TspRI CASTG 9 cut(s) 241, 337, 459, 853, 1612, 2245, 2620, 2635, 2855
Van91I CCANNNNNTGG 1 cut(s) 2333
VpaK11BI GGWCC 6 cut(s) 219, 712, 892, 1900, 2153, 2917
XapI RAATTY 6 cut(s) 761, 1027, 1617, 2264, 2345, 2368
XbaI TCTAGA 3 cut(s) 44, 80, 92
XceI RCATGY 2 cut(s) 1223, 2460
XmaJI CCTAGG 2 cut(s) 215, 1318
XmnI GAANNNNTTC 4 cut(s) 37, 311, 1787, 2541
ZrmI AGTACT 1 cut(s) 2952
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.