pycom12g18360

Regulatory photoreceptor which exists in two forms that are reversibly interconvertible by light the Pr form that absorbs maximally in the red region of the spectrum and the Pfr form that absorbs maximally in the far-red region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0007386
Physical Location & Seq
Forward (+)
20518723 .. 20522336
3614 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g18360.3

Sequence Viewer

Length: 3072 bp
ATGCTGACCACGGTCAGTCATGCAGTCCCAAGCGTTGGGGACTCCCCTGTCCTTGCCATGGGAACAGATATAAGGACAATTTTCACTGCCCCTAGTGCCTCCGCACTGCATAAGGCCCTCGGATTTGGAGAGGTTTCTCTCTTAAATCCCATCTTAGTTCATTGCAAGACCTCTGGCAAGCCTTTCTATGCAATAACTCATCGAGTAACGGGCAGTTTGATCGTTGACTTTGAGCCGGTGAAGCCTTATGAAGTGCCTATGACTGCTGCTGGGGCGCTGCAATCATACAAGCTTGCTGCCAAAGCAATTGCCAGGTTGCAGTCTTTGCCTAGCGGCAGCATGGAGAGGCTTTGTGATACAATGGTCCAAGAGGTTTTTGAACTCACCGGTTATGACAGGGTGATGACTTATAAATTTCATGATGATGATCATGGAGAAGTGGTCTCTGAGCTTACGAAGCCTGGCCTGGAGCCATATCTAGGGTTGCATTATCCATCCACTGACATACCTCAGGCTGCACGGTTCCTATTTATGAAGAATAAGGTCCGCATGATTGTTGATTGTGGTGCGAAACATGTGAAGGTGTTTCAAGATGAGAAGCTTCCGTTGGATCTCACATTGTGCGGTTCAACCCTGAGAGCCCCACATAGTTGCCATTTAGAATATATGAAGAATATGAATTCCATCGCATCTCTTGTTATGGCAGTAGTTGTTAATGAGGGGGATGACGAGGTGAGAGATCGATCTGATTCTGTTCCGCCTCAAAAAAGAAAGAGACTTTGGGGTTTGGTAGTATGCCATAATACAAGTCCAAGATTTGTTCCTTTCCCTCTCAGGTATGCCTGTGAGTTTTTAACTCAAGTGTTTGCCATCCATGTCAATAAGGAGATAGAGATGGAAGATCAGATGGTTGAGAAGAACATCCTGCGCACCCAAACGCTGTTGTGTGATATGCTGTTACGAGATGCACCCTTAGGTATTATGTCACAGAGCCCTAATGTTATGGATCTAGTGAAATGTGATGGAGCTGCCCTATTGTACAAGAACAAGGTATGGAGACTTGGAATGACTCTGAGTGACTTTCAGTTTCATGACATAGCTTCATGGCTCTCCGAGTACCATATGGATTCTACAGGTTTGAGTACAGATAGCTTGTATGATGCAGGATTCCCTGGGGCTCTTGCTCTTGGTGATGTAGTATGTGGAATGGCAGCTGTGTGGATAACTTCCAAGGACGTGCTCTTTTGGTTTCGATCCCACACTGCTGCGGAAATTCGATGGGGTGGTGCAAAACATGAAACCGGTGAGAAAGATGATGGTTCTAAAATGCACCCTAGATCATCATTCAAGGCTTTCCTTGAAGTTGTCAAGACGAGGAGCTTGCCTTGGAAGGACTATGAAATGGATGCAATTCATTCTTTGCAGCTTATTCTGAGGAATGCTTTCAAAGATGTCGAAACCACCTTGGAAGCAAACACCAAAGCAATCAATGTAAGGCTTAGTGACCTCAAACTTGAAGGGATGCAAGAACTGGAAGCTGTGACAAGTGAGATGGTCCGTTTAATTGAAACAGCCACGGTTCCAATTTTGGCTGTTGATGTTGATGGGCTGGTTAATGGGTGGAATACAAAAATCGATGAGTTAACTGGTCTTCCTGTTGATGAAGCAATCGGAAACCATTTGCTCACACTCGTGGAGGATTCTTCGACTAGCGTGGTGAAGAAAATGTTGGACTCAGCATTGCATGGCAAAGAGGAACAAAACATCCAATTCGAGATAAAAACACATGGTTCTAGGGCTGATCGATCTGATCCCATCAGCTTAGTTGTGAATGCTTGTGCAAGTAGAGACATTCGGGAAAATGTTGTGGGGGTGTGTTTTGTGGCACAGGATATCACTGGTCAGAAGAATGTGATGGACAAATTCACCCGGATCGAGGGTGATTACAAAGCAATTGTACAAAACCCTAGCCCCTTGATTCCCCCAATATTTGGTACAGATGAATTTGGCTGGTGTTCTGAGTGGAATTCAGCAATGACAAAGTTAACAGGGTGGAAACGAGAGGAGGTAATGGATAAAATGCTTCTGGGAGAGGTTTTTGCGATCAACATGGCATGTTGTCCTCTCAAGAGCCAAGAATCTTTCGTAAATCTTGGTATTGTACTTAACCATGCCATGATAGGTCTGGTGTCTGAAAAGGTTCCCTTTGGTTTCTGTGCTCGTAGTGGAAAACACATAGAATGCCTGCTGTGCGTGAGTAAGAAATTGAACAGCGAGGGCTCAGTCACTGGGGTCTTTTGCTTCTTGCAGCTTGCTAGCCCAGAGCTGCAACAAGCACTTCATGTCCAGCGCTTATCAGAACAAACTGCTGTGAAGAGATTGAAGGAATTGTCTTATATTAGAAGGCAGATCGATCAGTTTCGGAATCCTTTAGCCGGTATAATATTTTCTCGGAAAATGATGGAGGATACTGAGTTGGGAGCAGAACATCAACAGCTTCTGCATACGAGCGCCCAGTGCCAGCACCAGCTCAACAAAATACTGGATGATTCAGATCTTGATACCATCATTGACGGCTACCTGGACCTGGAGATGGTCGAGTTTACATTGCATGAAGTACTGAATGCCGCTGTCAGTCAAGTCATGATTAAGAGCAATGCCAAGAGCATCCGCATTGTCCACAATGCAGCGGAAGATATCATGACTGAGACGTTATATGGGGATAGTCTTAGGCTTCAACAAGTCGTAGCTGATTTCTTAGCGGTATCAGTAAGTTTTATGCCAACTGGAGGCCAGCTTACTATCGAAGCTAAGTTGACCAAAGATCAGTTAGGACAATCCGTTCAGCTCGTGCATTTGGAGCTCAGGATCACACACGCCGGTGGTGGGATTCCAGAAGGATTGCTGAACCAGATGTTTGGAAACAACGGAGACATATCTGAGGAGGGCATTAGCCTGCTCATCAGCAGTAAACTTGTGAAGCTCATGAATGGAGAAGTACGGTATCTTAGGGAAGAAGGCAAGTCAACCTTCATGATATCCGTAGAACTCGCCGTGGCAAACAAGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0004672 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0006417 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007275 GO:0007602 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009581 GO:0009582 GO:0009583 GO:0009585 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009637 GO:0009638 GO:0009639 GO:0009640 GO:0009642 GO:0009791 GO:0009881 GO:0009883 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010017 GO:0010018 GO:0010114 GO:0010161 GO:0010201 GO:0010203 GO:0010218 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0016301 GO:0016310 GO:0016604 GO:0016740 GO:0016772 GO:0016773 GO:0017148 GO:0019222 GO:0019538 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031516 GO:0031974 GO:0031981 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0034248 GO:0034249 GO:0036211 GO:0038023 GO:0042221 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046685 GO:0048519 GO:0048523 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051606 GO:0051716 GO:0055122 GO:0060089 GO:0060255 GO:0065007 GO:0070013 GO:0071214 GO:0071478 GO:0071482 GO:0071489 GO:0071490 GO:0071491 GO:0071704 GO:0080090 GO:0097159 GO:0104004 GO:0140096 GO:1901363 GO:1901564 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
ko04712 map04712
Pfam Domains
Protein Families
GAF HATPase_c HisKA PAS PAS_2 PHY

Protein Analysis

1024

Amino Acids

113.56

Weight (kDa)

5.6

Isoelectric Point (pI)

44.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 411
AasI GACNNNNNNGTC 1 cut(s) 47
Acc16I TGCGCA 1 cut(s) 929
AccB7I CCANNNNNTGG 2 cut(s) 35, 1991
AclWI GGATC 6 cut(s) 618, 1014, 1248, 1805, 1940, 2876
AcsI RAATTY 6 cut(s) 413, 679, 1272, 1922, 2003, 2026
AdeI CACNNNGTG 1 cut(s) 621
AfaI GTAC 8 cut(s) 1040, 1118, 1144, 1959, 1996, 2163, 2619, 3000
AfeI AGCGCT 1 cut(s) 2351
AflIII ACRYGT 1 cut(s) 574
AgeI ACCGGT 2 cut(s) 386, 1301
AjiI CACGTC 1 cut(s) 1237
AjnI CCWGG 6 cut(s) 311, 460, 465, 1171, 2580, 2586
AjuI GAANNNNNNNTTGG 6 cut(s) 590, 622, 763, 795, 1712, 1744
AleI CACNNNNGTG 2 cut(s) 1691, 2880
AloI GAACNNNNNNTCC 4 cut(s) 1749, 1781, 2826, 2858
Alw21I GWGCWC 3 cut(s) 1242, 2221, 2865
Alw26I GTCTC 6 cut(s) 448, 769, 1051, 1842, 2702, 2925
AlwI GGATC 6 cut(s) 618, 1014, 1248, 1805, 1940, 2876
AlwNI CAGNNNCTG 2 cut(s) 2288, 2500
Aor51HI AGCGCT 1 cut(s) 2351
AoxI GGCC 3 cut(s) 114, 463, 2791
ApoI RAATTY 6 cut(s) 413, 679, 1272, 1922, 2003, 2026
AsiGI ACCGGT 2 cut(s) 386, 1301
Asp700I GAANNNNTTC 2 cut(s) 1442, 2199
AspLEI GCGC 4 cut(s) 277, 930, 2352, 2513
AspS9I GGNCC 5 cut(s) 115, 364, 544, 1555, 2584
AsuC2I CCSGG 1 cut(s) 1930
AsuHPI GGTGA 9 cut(s) 250, 376, 412, 745, 1202, 1316, 1729, 1918, 1952
AsuNHI GCTAGC 1 cut(s) 2315
AvaII GGWCC 4 cut(s) 364, 544, 1555, 2584
AxyI CCTNAGG 2 cut(s) 510, 973
BaeI ACNNNNGTAYC 2 cut(s) 348, 381
BanII GRGCYC 5 cut(s) 643, 995, 1180, 2282, 2865
BauI CACGAG 2 cut(s) 1691, 2849
BbsI GAAGAC 1 cut(s) 1643
Bbv12I GWGCWC 3 cut(s) 1242, 2221, 2865
BceAI ACGGC 2 cut(s) 2590, 3038
BcgI CGANNNNNNTGC 4 cut(s) 202, 236, 1363, 1397
BciT130I CCWGG 6 cut(s) 313, 462, 467, 1173, 2582, 2588
BciVI GTATCC 1 cut(s) 2461
BclI TGATCA 1 cut(s) 427
BcnI CCSGG 1 cut(s) 1930
BcoDI GTCTC 6 cut(s) 448, 769, 1051, 1842, 2702, 2925
BfaI CTAG 9 cut(s) 93, 330, 479, 1010, 1335, 1710, 1794, 1968, 2316
BfmI CTRYAG 1 cut(s) 1131
BfoI RGCGCY 3 cut(s) 278, 2353, 2514
BfuI GTATCC 1 cut(s) 2461
BglII AGATCT 1 cut(s) 2554
BmcAI AGTACT 1 cut(s) 2619
Bme1390I CCNGG 7 cut(s) 313, 462, 467, 1173, 1930, 2582, 2588
Bme18I GGWCC 4 cut(s) 364, 544, 1555, 2584
BmgBI CACGTC 1 cut(s) 1237
BmgT120I GGNCC 5 cut(s) 115, 364, 544, 1555, 2584
BmiI GGNNCC 4 cut(s) 471, 524, 1581, 2202
BmrFI CCNGG 7 cut(s) 313, 462, 467, 1173, 1930, 2582, 2588
BmrI ACTGGG 2 cut(s) 2298, 2509
BmsI GCATC 6 cut(s) 698, 955, 1150, 1396, 1512, 2676
BmtI GCTAGC 1 cut(s) 2319
BmuI ACTGGG 2 cut(s) 2298, 2509
BoxI GACNNNNGTC 1 cut(s) 11
BpiI GAAGAC 1 cut(s) 1643
BpmI CTGGAG 3 cut(s) 488, 2609, 2808
Bpu10I CCTNAGC 1 cut(s) 2864
BpuEI CTTGAG 2 cut(s) 843, 2111
BpuMI CCSGG 1 cut(s) 1930
Bsa29I ATCGAT 4 cut(s) 742, 1635, 1804, 2412
BsaBI GATNNNNATC 2 cut(s) 426, 2553
BsaI GGTCTC 1 cut(s) 448
BsaWI WCCGGW 2 cut(s) 386, 1301
BsaXI ACNNNNNCTCC 2 cut(s) 426, 456
Bse118I RCCGGY 5 cut(s) 235, 386, 1301, 2435, 2879
Bse1I ACTGG 7 cut(s) 1536, 1651, 1903, 2293, 2515, 2547, 2791
Bse21I CCTNAGG 2 cut(s) 510, 973
Bse3DI GCAATG 5 cut(s) 160, 1739, 2040, 2606, 2662
Bse8I GATNNNNATC 2 cut(s) 426, 2553
BseBI CCWGG 6 cut(s) 313, 462, 467, 1173, 2582, 2588
BseCI ATCGAT 4 cut(s) 742, 1635, 1804, 2412
BseGI GGATG 9 cut(s) 494, 730, 870, 921, 1411, 1527, 1764, 2551, 2667
BseJI GATNNNNATC 2 cut(s) 426, 2553
BseMI GCAATG 5 cut(s) 160, 1739, 2040, 2606, 2662
BseNI ACTGG 7 cut(s) 1536, 1651, 1903, 2293, 2515, 2547, 2791
BseRI GAGGAG 3 cut(s) 1390, 2078, 2957
BseYI CCCAGC 1 cut(s) 269
BsgI GTGCAG 1 cut(s) 501
BshFI GGCC 3 cut(s) 116, 465, 2793
BshTI ACCGGT 2 cut(s) 386, 1301
BshVI ATCGAT 4 cut(s) 742, 1635, 1804, 2412
BsiHKAI GWGCWC 3 cut(s) 1242, 2221, 2865
BsiSI CCGG 6 cut(s) 236, 387, 1302, 1930, 2436, 2880
BslFI GGGAC 2 cut(s) 11, 53
BsmAI GTCTC 6 cut(s) 448, 769, 1051, 1842, 2702, 2925
BsmBI CGTCTC 1 cut(s) 2702
BsmFI GGGAC 2 cut(s) 11, 53
BsmI GAATGC 4 cut(s) 1444, 1837, 2246, 2629
BsnI GGCC 3 cut(s) 116, 465, 2793
Bso31I GGTCTC 1 cut(s) 448
Bsp1286I GDGCHC 7 cut(s) 643, 995, 1180, 1242, 2221, 2282, 2865
Bsp1407I TGTACA 2 cut(s) 1038, 1957
Bsp19I CCATGG 1 cut(s) 57
BspANI GGCC 3 cut(s) 116, 465, 2793
BspDI ATCGAT 4 cut(s) 742, 1635, 1804, 2412
BspHI TCATGA 6 cut(s) 418, 1090, 2643, 2700, 2985, 3033
BspLI GGNNCC 4 cut(s) 471, 524, 1581, 2202
BspOI GCTAGC 1 cut(s) 2319
BspPI GGATC 6 cut(s) 618, 1014, 1248, 1805, 1940, 2876
BspTNI GGTCTC 1 cut(s) 448
BsrDI GCAATG 5 cut(s) 160, 1739, 2040, 2606, 2662
BsrFI RCCGGY 5 cut(s) 235, 386, 1301, 2435, 2879
BsrGI TGTACA 2 cut(s) 1038, 1957
BsrI ACTGG 7 cut(s) 1536, 1651, 1903, 2293, 2515, 2547, 2791
BssAI RCCGGY 5 cut(s) 235, 386, 1301, 2435, 2879
BssSI CACGAG 2 cut(s) 1691, 2849
BssT1I CCWWGG 4 cut(s) 57, 1230, 1385, 1464
Bst2BI CACGAG 2 cut(s) 1691, 2849
Bst2UI CCWGG 6 cut(s) 313, 462, 467, 1173, 2582, 2588
Bst4CI ACNGT 4 cut(s) 13, 522, 1579, 3003
Bst6I CTCTTC 1 cut(s) 2369
BstAPI GCANNNNNTGC 1 cut(s) 325
BstAUI TGTACA 2 cut(s) 1038, 1957
BstC8I GCNNGC 9 cut(s) 179, 294, 1382, 2246, 2313, 2317, 2522, 2795, 2957
BstDSI CCRYGG 4 cut(s) 9, 57, 1575, 3054
BstF5I GGATG 9 cut(s) 494, 730, 870, 921, 1411, 1527, 1764, 2551, 2667
BstH2I RGCGCY 3 cut(s) 278, 2353, 2514
BstHHI GCGC 4 cut(s) 277, 930, 2352, 2513
BstMAI GTCTC 6 cut(s) 448, 769, 1051, 1842, 2702, 2925
BstMWI GCNNNNNNNGC 9 cut(s) 95, 241, 272, 302, 325, 457, 2250, 2517, 2860
BstNI CCWGG 6 cut(s) 313, 462, 467, 1173, 2582, 2588
BstNSI RCATGY 2 cut(s) 578, 2118
BstPAI GACNNNNGTC 1 cut(s) 11
BstSCI CCNGG 7 cut(s) 311, 460, 465, 1171, 1928, 2580, 2586
BstSFI CTRYAG 1 cut(s) 1131
BstV2I GAAGAC 1 cut(s) 1643
BstX2I RGATCY 3 cut(s) 610, 1006, 2554
BstXI CCANNNNNNTGG 1 cut(s) 2918
BstYI RGATCY 3 cut(s) 610, 1006, 2554
Bsu15I ATCGAT 4 cut(s) 742, 1635, 1804, 2412
Bsu36I CCTNAGG 2 cut(s) 510, 973
BsuI GTATCC 1 cut(s) 2461
BsuRI GGCC 3 cut(s) 116, 465, 2793
BsuTUI ATCGAT 4 cut(s) 742, 1635, 1804, 2412
BtgI CCRYGG 4 cut(s) 9, 57, 1575, 3054
BtgZI GCGATG 1 cut(s) 670
BtrI CACGTC 1 cut(s) 1237
BtsCI GGATG 9 cut(s) 494, 730, 870, 921, 1411, 1527, 1764, 2551, 2667
BtsI GCAGTG 3 cut(s) 84, 104, 1260
BtsIMutI CAGTG 7 cut(s) 84, 104, 498, 1260, 1896, 2286, 2522
Cac8I GCNNGC 9 cut(s) 179, 294, 1382, 2246, 2313, 2317, 2522, 2795, 2957
CaiI CAGNNNCTG 2 cut(s) 2288, 2500
CciI TCATGA 6 cut(s) 418, 1090, 2643, 2700, 2985, 3033
CfoI GCGC 4 cut(s) 277, 930, 2352, 2513
Cfr10I RCCGGY 5 cut(s) 235, 386, 1301, 2435, 2879
Cfr13I GGNCC 5 cut(s) 115, 364, 544, 1555, 2584
ClaI ATCGAT 4 cut(s) 742, 1635, 1804, 2412
Csp6I GTAC 8 cut(s) 1039, 1117, 1143, 1958, 1995, 2162, 2618, 2999
CspAI ACCGGT 2 cut(s) 386, 1301
CviQI GTAC 8 cut(s) 1039, 1117, 1143, 1958, 1995, 2162, 2618, 2999
DraIII CACNNNGTG 1 cut(s) 621
DrdI GACNNNNNNGTC 1 cut(s) 47
DseDI GACNNNNNNGTC 1 cut(s) 47
Eam1104I CTCTTC 1 cut(s) 2369
EarI CTCTTC 1 cut(s) 2369
EciI GGCGGA 1 cut(s) 747
Ecl136II GAGCTC 1 cut(s) 2863
Eco130I CCWWGG 4 cut(s) 57, 1230, 1385, 1464
Eco24I GRGCYC 5 cut(s) 643, 995, 1180, 2282, 2865
Eco31I GGTCTC 1 cut(s) 448
Eco32I GATATC 3 cut(s) 1894, 2698, 3039
Eco47I GGWCC 4 cut(s) 364, 544, 1555, 2584
Eco47III AGCGCT 1 cut(s) 2351
Eco53kI GAGCTC 1 cut(s) 2863
Eco81I CCTNAGG 2 cut(s) 510, 973
EcoICRI GAGCTC 1 cut(s) 2863
EcoO109I RGGNCCY 1 cut(s) 115
EcoRI GAATTC 2 cut(s) 679, 2026
EcoRII CCWGG 6 cut(s) 311, 460, 465, 1171, 2580, 2586
EcoRV GATATC 3 cut(s) 1894, 2698, 3039
EcoT14I CCWWGG 4 cut(s) 57, 1230, 1385, 1464
EcoT38I GRGCYC 5 cut(s) 643, 995, 1180, 2282, 2865
ErhI CCWWGG 4 cut(s) 57, 1230, 1385, 1464
Esp3I CGTCTC 1 cut(s) 2702
FalI AAGNNNNNCTT 4 cut(s) 2189, 2221, 3014, 3046
FaqI GGGAC 2 cut(s) 11, 53
FauNDI CATATG 1 cut(s) 1122
FbaI TGATCA 1 cut(s) 427
FokI GGATG 9 cut(s) 481, 737, 857, 908, 1418, 1534, 1751, 2558, 2654
FriOI GRGCYC 5 cut(s) 643, 995, 1180, 2282, 2865
FspBI CTAG 9 cut(s) 93, 330, 479, 1010, 1335, 1710, 1794, 1968, 2316
FspI TGCGCA 1 cut(s) 929
GlaI GCGC 4 cut(s) 276, 929, 2351, 2512
GsaI CCCAGC 1 cut(s) 273
GsuI CTGGAG 3 cut(s) 488, 2609, 2808
HaeII RGCGCY 3 cut(s) 278, 2353, 2514
HaeIII GGCC 3 cut(s) 116, 465, 2793
HapII CCGG 6 cut(s) 236, 387, 1302, 1930, 2436, 2880
HhaI GCGC 4 cut(s) 277, 930, 2352, 2513
Hin6I GCGC 4 cut(s) 275, 928, 2350, 2511
HinP1I GCGC 4 cut(s) 275, 928, 2350, 2511
HincII GTYRAC 5 cut(s) 226, 1644, 2046, 2817, 3027
HindII GTYRAC 5 cut(s) 226, 1644, 2046, 2817, 3027
HindIII AAGCTT 2 cut(s) 290, 599
HpaI GTTAAC 2 cut(s) 1644, 2046
HpaII CCGG 6 cut(s) 236, 387, 1302, 1930, 2436, 2880
HphI GGTGA 9 cut(s) 250, 376, 412, 745, 1202, 1316, 1729, 1918, 1952
Hpy166II GTNNAC 8 cut(s) 226, 1644, 2046, 2604, 2680, 2817, 2972, 3027
Hpy8I GTNNAC 8 cut(s) 226, 1644, 2046, 2604, 2680, 2817, 2972, 3027
HpyAV CCTTC 8 cut(s) 574, 1384, 1511, 2377, 2397, 2891, 3011, 3040
HpyCH4III ACNGT 4 cut(s) 13, 522, 1579, 3003
HpyCH4IV ACGT 2 cut(s) 1236, 2711
HpyF10VI GCNNNNNNNGC 9 cut(s) 95, 241, 272, 302, 325, 457, 2250, 2517, 2860
HpySE526I ACGT 2 cut(s) 1236, 2711
HspAI GCGC 4 cut(s) 275, 928, 2350, 2511
Ksp22I TGATCA 1 cut(s) 427
KspAI GTTAAC 2 cut(s) 1644, 2046
LmnI GCTCC 5 cut(s) 469, 1025, 1377, 2480, 2860
LweI GCATC 6 cut(s) 698, 955, 1150, 1396, 1512, 2676
MaeI CTAG 9 cut(s) 93, 330, 479, 1010, 1335, 1710, 1794, 1968, 2316
MaeII ACGT 2 cut(s) 1236, 2711
MaeIII GTNAC 7 cut(s) 205, 957, 984, 1076, 1502, 1540, 2284
MfeI CAATTG 2 cut(s) 306, 1953
MflI RGATCY 3 cut(s) 610, 1006, 2554
MhlI GDGCHC 7 cut(s) 643, 995, 1180, 1242, 2221, 2282, 2865
MlyI GAGTC 3 cut(s) 35, 1063, 1727
MmeI TCCRAC 2 cut(s) 588, 1710
MroXI GAANNNNTTC 2 cut(s) 1442, 2199
MseI TTAA 9 cut(s) 143, 714, 854, 1562, 1614, 1643, 2045, 2166, 2649
MslI CAYNNNNRTG 3 cut(s) 423, 1691, 2880
MspA1I CMGCKG 3 cut(s) 1214, 2630, 2690
MspI CCGG 6 cut(s) 236, 387, 1302, 1930, 2436, 2880
MspR9I CCNGG 7 cut(s) 313, 462, 467, 1173, 1930, 2582, 2588
MunI CAATTG 2 cut(s) 306, 1953
Mva1269I GAATGC 4 cut(s) 1444, 1837, 2246, 2629
MvaI CCWGG 6 cut(s) 313, 462, 467, 1173, 2582, 2588
MwoI GCNNNNNNNGC 9 cut(s) 95, 241, 272, 302, 325, 457, 2250, 2517, 2860
NciI CCSGG 1 cut(s) 1930
NcoI CCATGG 1 cut(s) 57
NdeI CATATG 1 cut(s) 1122
NheI GCTAGC 1 cut(s) 2315
NlaIV GGNNCC 4 cut(s) 471, 524, 1581, 2202
NmuCI GTSAC 5 cut(s) 984, 1076, 1502, 1540, 2284
NsbI TGCGCA 1 cut(s) 929
NspI RCATGY 2 cut(s) 578, 2118
OliI CACNNNNGTG 2 cut(s) 1691, 2880
PagI TCATGA 6 cut(s) 418, 1090, 2643, 2700, 2985, 3033
PasI CCCWGGG 1 cut(s) 1172
PciI ACATGT 1 cut(s) 574
PctI GAATGC 4 cut(s) 1444, 1837, 2246, 2629
PdmI GAANNNNTTC 2 cut(s) 1442, 2199
PfeI GAWTC 9 cut(s) 749, 1127, 1167, 1700, 1978, 2138, 2425, 2549, 2890
PflMI CCANNNNNTGG 2 cut(s) 35, 1991
PinAI ACCGGT 2 cut(s) 386, 1301
PleI GAGTC 3 cut(s) 35, 1063, 1727
PpsI GAGTC 3 cut(s) 35, 1063, 1727
PscI ACATGT 1 cut(s) 574
PshAI GACNNNNGTC 1 cut(s) 11
PsiI TTATAA 1 cut(s) 411
Psp124BI GAGCTC 1 cut(s) 2865
Psp6I CCWGG 6 cut(s) 311, 460, 465, 1171, 2580, 2586
PspFI CCCAGC 1 cut(s) 269
PspGI CCWGG 6 cut(s) 311, 460, 465, 1171, 2580, 2586
PspN4I GGNNCC 4 cut(s) 471, 524, 1581, 2202
PspPI GGNCC 5 cut(s) 115, 364, 544, 1555, 2584
PstNI CAGNNNCTG 2 cut(s) 2288, 2500
PsuI RGATCY 3 cut(s) 610, 1006, 2554
PvuII CAGCTG 1 cut(s) 1214
RsaI GTAC 8 cut(s) 1040, 1118, 1144, 1959, 1996, 2163, 2619, 3000
RsaNI GTAC 8 cut(s) 1039, 1117, 1143, 1958, 1995, 2162, 2618, 2999
RseI CAYNNNNRTG 3 cut(s) 423, 1691, 2880
SacI GAGCTC 1 cut(s) 2865
SaqAI TTAA 9 cut(s) 143, 714, 854, 1562, 1614, 1643, 2045, 2166, 2649
Sau96I GGNCC 5 cut(s) 115, 364, 544, 1555, 2584
ScaI AGTACT 1 cut(s) 2619
SchI GAGTC 3 cut(s) 35, 1063, 1727
ScrFI CCNGG 7 cut(s) 313, 462, 467, 1173, 1930, 2582, 2588
SduI GDGCHC 7 cut(s) 643, 995, 1180, 1242, 2221, 2282, 2865
SfaNI GCATC 6 cut(s) 698, 955, 1150, 1396, 1512, 2676
SfcI CTRYAG 1 cut(s) 1131
SgrAI CRCCGGYG 1 cut(s) 2879
SinI GGWCC 4 cut(s) 364, 544, 1555, 2584
SmiMI CAYNNNNRTG 3 cut(s) 423, 1691, 2880
SmlI CTYRAG 2 cut(s) 858, 2126
SmoI CTYRAG 2 cut(s) 858, 2126
SspI AATATT 2 cut(s) 1989, 2445
SspMI CTAG 9 cut(s) 93, 330, 479, 1010, 1335, 1710, 1794, 1968, 2316
SstI GAGCTC 1 cut(s) 2865
StyD4I CCNGG 7 cut(s) 311, 460, 465, 1171, 1928, 2580, 2586
StyI CCWWGG 4 cut(s) 57, 1230, 1385, 1464
TaaI ACNGT 4 cut(s) 13, 522, 1579, 3003
TaiI ACGT 2 cut(s) 1239, 2714
TatI WGTACW 5 cut(s) 1038, 1142, 1957, 2161, 2617
TauI GCSGC 2 cut(s) 336, 2630
TfiI GAWTC 9 cut(s) 749, 1127, 1167, 1700, 1978, 2138, 2425, 2549, 2890
Tru1I TTAA 9 cut(s) 143, 714, 854, 1562, 1614, 1643, 2045, 2166, 2649
Tru9I TTAA 9 cut(s) 143, 714, 854, 1562, 1614, 1643, 2045, 2166, 2649
TscAI CASTG 7 cut(s) 91, 111, 505, 1267, 1903, 2293, 2522
TseFI GTSAC 5 cut(s) 984, 1076, 1502, 1540, 2284
Tsp45I GTSAC 5 cut(s) 984, 1076, 1502, 1540, 2284
TspGWI ACGGA 5 cut(s) 594, 1547, 2830, 2943, 3031
TspRI CASTG 7 cut(s) 91, 111, 505, 1267, 1903, 2293, 2522
Van91I CCANNNNNTGG 2 cut(s) 35, 1991
VpaK11BI GGWCC 4 cut(s) 364, 544, 1555, 2584
XapI RAATTY 6 cut(s) 413, 679, 1272, 1922, 2003, 2026
XceI RCATGY 2 cut(s) 578, 2118
XcmI CCANNNNNNNNNTGG 1 cut(s) 2182
XmnI GAANNNNTTC 2 cut(s) 1442, 2199
XspI CTAG 9 cut(s) 93, 330, 479, 1010, 1335, 1710, 1794, 1968, 2316
ZrmI AGTACT 1 cut(s) 2619
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.