Rroxscaffold_6G00420590

Regulatory photoreceptor which exists in two forms that are reversibly interconvertible by light the Pr form that absorbs maximally in the red region of the spectrum and the Pfr form that absorbs maximally in the far-red region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0007386
Physical Location & Seq
Forward (+)
41780029 .. 41786075
6047 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00420590.1

Sequence Viewer

Length: 3432 bp
ATGTCGTCTTCAAGACCCAGTCACTCATCCAGCAATTCAGGGAGATCAAGGCACACTGCTAGAGTTATTGCTCAGACAACTGTAGATGCAAAGCTTCATGCAGATTTTGAGGAGTCGGGCAGTTCCTTTGACTACTCGAGCTCAGTGCGTGTTAGCAGTTCAGTTGCTGGGGATCAACAACCGAGGTCTGACAAAGTGACCACTGCTTATCTCCATCACATACAGAAAGGGAAGTTGATCCAGCCATTTGGCTGCTTGCTAGCCTTAGATGACAAAACTTTCAGGGTCATTGCATACAGTGAGAATGCCCCTGAAATGCTGACTATGGTTAGTCATGCCGTCCCAAGTGTCGGGGACCACCCAGTTCTTGGCATAGGAACAGATGTAAGAACAATTTTCACAGGCCCTAGTGCCTCTGCATTGCATAAGGCCCTCGGGTTCGGGGAGGTTTCTCTCTTAAATCCCATCTTAGTCCATTGCAAGTCATCCGGAAAGCCTTTCTATGCTATAGTCCACCGGGTAACAGGTAGCTTGGTCATTGATTTTGAACCGGTGAAGCCTTATGAAGTCCCCATGACTGCTGCTGGAGCTCTGCAATCATACAAACTTGCAGCCAAAGCAATTGCACGATTGCAGTCTTTGCCTAGTGGGAGCCTGGAGAGGCTTTGTGATACAATGGTTCAAGAGGTTTTTGAGCTCACTGGTTATGACAGGGTGATGGCCTATAAATTTCATGATGATGATCATGGGGAAGTTATTGCAGAGCTTACGAAGCCTGGCCTGGAGCCATATCTGGGTTTGCATTATCCATCCACTGATATACCTCAAGCTTCACGGTTCCTGTTTATGAAGAATAAGGTCCGTATGATTGTTGACTGTCGTGCTAAAAATGTCAAGGTCCTTCAAGATGAGAAACTTCCTTTGGATCTAACATTGTGTGGTTCAACCCTCAGAGCCCCACATAGTTGCCATTTGCTATATATGGAGAACATGAATTCCATTGCTTCTCTTGTTATGGCAGTAGTGGTCAATGAAGGGGAAGGGGATGATGAGGTTGCCGGTCCTGATGCTGCGCAGACTCAAAAGAGAAAGAGACTGTGGGGTTTAGTAGTATGTCATAACACAACTCCAAGGTTTGTCCCTTTCCCTCTCAGATATGCCTGTGAGTTTCTAGCTCAAGTGTTTGCCATCCATGTGAATAAGGAATTAGAGTTGGAGGATCAGATTATTGAAAAGAACATCCTGCGTACCCAAACGCTGTTGTGTGATATGCTTTTGCGCGATGCTCCTTTGGGTATAGTGTCACAAACCCCTAATATTATGGATCTAGTGAAATGTGATGGGGCTGCCCTGCTATACAAGAACAAGGTATGGAGACTTGGATTAACTCCAAGTGACTTCCAGATTCAGGACATATCCTTGTGGCTCACTGAGTGCCATATGGATTCCACAGGTTTGAGTACAGATAGCTTGTATGACGCGGGTTTCCCTGGCGCTCTCGCTCTTGGTGATGTAGTGTGTGGAATGGCAGCTGTGAAGATAACTTCCAAGGACACAATTTTTTGGTTCCGATCCCACACTGCTGCAGAAATCCGATGGGGTGGTGCAAAACATGACCCGGATGAGAAGGACGATGGCTGGAAAATGCACCCCAGATCATCATTCAAGGCTTTCCTTGAAGTTGTGAAGACAAGGAGCTTACCTTGGAAGGACTATGAAATGGATGCAATTCATTCTTTGCAGCTTATTCTGAGGAATGCATTCAAGGATGTGGGAACCATGGATGTAAACAACAGTGGAATCCAGATGAGGCTTAGTGACCTGAAAGTTGATGGGATGCAAGAACTGGAAGCAGTGACAGGTGAGATGGTCCGGTTGATTGAAACAGCAACAGTGCCAATTTTGGCAGTTGATGTTGATGGGTCAGTTAACGGGTGGAACACAAAAATTTCCGAGTTAACTGGTCTTCCTGTTGATGAAGCAATTGGAAAGAATTTACTCACACTTGTGGAAGAATCTTCAACTAGCATGGTGGAAAGGATGCTGGAGTTGGCATTGCAAGGCAAAGAGGAACAAAACATCCAATTCGAGATAAAAACACATGGTGCTAGGACTGACTTTGGTCCCATCAGGTTAGTTGTGAATGCTTGTGCAAGCAGAGATATTCGTGAAAATGTTGTGGGAGTGTGTTTTGTGGCCCAAGATATCACTGGTCAGAAGACTGTGATGGACAAGTTCACTCGCATTGAAGGTGATTATAAAGCAATTGTACAAAACCCAAGTCCGTTGATCCCCCCAATATTTGGCACTGATGAATTTGGCTGGTGTTCTGAGTGGAATCCAGCAATGACTAAGTTAACTGGGTGGAAACGAGAGGAGGTAATGGATAAAATGCTGTTGGGGGAGGTTTTTGGGATCAACATGGCTTGTTGTCCTCTCAAGAATCAAGAAGCTTTCGTAAATCTTGGTGTTGTAATCAATCACGCCATGACTGGTCAGGTGCCTGAAAAGGTTCCTTTTGGTTTCTGGAATCGAGGTGGAAAGTACACTGAATGTTTGTTGTGTGTAAGTAAGAAATTGGACAGTGAGGGTGCTGTCACTGGGGTCTTTTGCTTCTTGCAGCTTGCTAGCCCAGAATTGCAACAAGCACTTCATGTCCAACGCTTATCTGAACAAACCGCTATGAAGAGATTAAAAGCATTAGCTTATATTAAAAGGCAGATCAGGAATCCTTTATCTGGAATACTATTTTCTCGGAAAATGATCGAGGGTACTGAGTTGGGAGCAGAACAAAAAAGCTCGTGCAAACCAGTGCCCAGTGCCAGCAGCAGCTCAGCAAAATACTTGACGACTCTGATCTTGATAGCATCATTGATGGGTATGACAAACTACTTGGACCTGGAAATGGTTGAGTTCACTTTGCATGAAGTACTGGTTGCCTCTATTAGTCAAGTCATGATTAAGAGCAACGCCAAGAGTATCCCCATTGTCCAAGATGCAAATGAAGAGATCATGACTGAGACATTGTATGGCGATAGTCTTAGGCTTCAACAAGTCTTGGCTGATTTCTTGTCAGTGTCAGTTAATTATATGCCAACCGGAGGCCAGCTTACTGTAGCAGTTAATTTGACAAAAGATCAGCTGGGACAATCTGTTCATCTCGTACATTTGGAACTCAGTGAGTATCACTCATCAAATGCTGAGTTGATATGTTGGATCAGGATATCACATGGGGGTAGTGGGATTCCAGAAGGATTACTGAACCAGATGTTTGGAACAGATGGAGACGTATCTGAAGAAGGCATTAGCCTACTCATAAGCAGAAAACTAGTGAAGCTCATGAATGGAGATGTACAGTACCTCCGGGAAGCAGGAAAGTCAACCTTCATTATATCTGCTGAACTTGCTGCTGCACATAAGATGAGGTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0004672 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0006417 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007275 GO:0007602 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009581 GO:0009582 GO:0009583 GO:0009585 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009637 GO:0009638 GO:0009639 GO:0009640 GO:0009642 GO:0009791 GO:0009881 GO:0009883 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010017 GO:0010018 GO:0010114 GO:0010161 GO:0010201 GO:0010203 GO:0010218 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0016301 GO:0016310 GO:0016604 GO:0016740 GO:0016772 GO:0016773 GO:0017148 GO:0019222 GO:0019538 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031516 GO:0031974 GO:0031981 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0034248 GO:0034249 GO:0036211 GO:0038023 GO:0042221 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046685 GO:0048519 GO:0048523 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051606 GO:0051716 GO:0055122 GO:0060089 GO:0060255 GO:0065007 GO:0070013 GO:0071214 GO:0071478 GO:0071482 GO:0071489 GO:0071490 GO:0071491 GO:0071704 GO:0080090 GO:0097159 GO:0104004 GO:0140096 GO:1901363 GO:1901564 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
ko04712 map04712
Pfam Domains
Protein Families
GAF HATPase_c HisKA PAS PAS_2 PHY

Protein Analysis

1143

Amino Acids

125.98

Weight (kDa)

5.83

Isoelectric Point (pI)

44.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS_2 PF08446 69 - 185 3.5e-35 PAS fold
GAF PF01590 219 - 402 1.1e-15 GAF domain
PHY PF00360 416 - 589 1.1e-54 Phytochrome region
PAS PF00989 622 - 736 2e-23 PAS fold
PAS_4 PF08448 630 - 740 7.7e-07 PAS fold
PAS PF00989 752 - 874 4.6e-20 PAS fold
HATPase_c PF02518 1011 - 1132 4e-10 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2259
Acc16I TGCGCA 1 cut(s) 1074
AccB1I GGYRCC 1 cut(s) 2500
AccB7I CCANNNNNTGG 2 cut(s) 368, 2303
AccII CGCG 2 cut(s) 1281, 1481
AccIII TCCGGA 1 cut(s) 488
AciI CCGC 2 cut(s) 1481, 2679
AclWI GGATC 9 cut(s) 180, 232, 933, 1227, 1332, 1566, 2284, 2423, 3224
AcsI RAATTY 5 cut(s) 728, 994, 1947, 1993, 2315
AcuI CTGAAG 1 cut(s) 3315
AdeI CACNNNGTG 2 cut(s) 1434, 2105
AfaI GTAC 9 cut(s) 1249, 1462, 2271, 2546, 2773, 2931, 3165, 3354, 3359
AfiI CCNNNNNNNGG 8 cut(s) 350, 368, 794, 1408, 2303, 2738, 2905, 3101
AgeI ACCGGT 1 cut(s) 550
AhlI ACTAGT 1 cut(s) 3328
AjnI CCWGG 5 cut(s) 654, 775, 780, 1489, 2898
AjuI GAANNNNNNNTTGG 2 cut(s) 905, 937
AleI CACNNNNGTG 1 cut(s) 2006
AloI GAACNNNNNNTCC 6 cut(s) 980, 1012, 2064, 2096, 3138, 3170
Alw21I GWGCWC 3 cut(s) 143, 592, 699
Alw26I GTCTC 4 cut(s) 1087, 1369, 3014, 3279
AlwI GGATC 9 cut(s) 180, 232, 933, 1227, 1332, 1566, 2284, 2423, 3224
AlwNI CAGNNNCTG 1 cut(s) 167
Ama87I CYCGRG 2 cut(s) 136, 434
Aor13HI TCCGGA 1 cut(s) 488
AoxI GGCC 6 cut(s) 403, 429, 720, 778, 2196, 3103
ApoI RAATTY 5 cut(s) 728, 994, 1947, 1993, 2315
ArsI GACNNNNNNTTYG 2 cut(s) 2266, 2298
AsiGI ACCGGT 1 cut(s) 550
Asp700I GAANNNNTTC 2 cut(s) 1760, 2511
AspLEI GCGC 3 cut(s) 1075, 1281, 1496
AsuC2I CCSGG 3 cut(s) 518, 1619, 3365
AsuHPI GGTGA 5 cut(s) 565, 727, 1520, 1874, 2264
AsuNHI GCTAGC 2 cut(s) 259, 2627
AvaI CYCGRG 2 cut(s) 136, 434
AvaII GGWCC 8 cut(s) 355, 859, 898, 1061, 1870, 2123, 2896, 3426
BaeGI GKGCMC 1 cut(s) 2817
BaeI ACNNNNGTAYC 2 cut(s) 663, 696
BanI GGYRCC 1 cut(s) 2500
BanII GRGCYC 4 cut(s) 143, 592, 699, 958
BauI CACGAG 1 cut(s) 2800
BbsI GAAGAC 3 cut(s) 1694, 1958, 2225
Bbv12I GWGCWC 3 cut(s) 143, 592, 699
BceAI ACGGC 1 cut(s) 323
BcgI CGANNNNNNTGC 2 cut(s) 127, 161
BciT130I CCWGG 5 cut(s) 656, 777, 782, 1491, 2900
BciVI GTATCC 1 cut(s) 2990
BclI TGATCA 1 cut(s) 742
BcnI CCSGG 3 cut(s) 518, 1619, 3365
BcoDI GTCTC 4 cut(s) 1087, 1369, 3014, 3279
BcuI ACTAGT 1 cut(s) 3328
BfmI CTRYAG 4 cut(s) 81, 507, 1584, 3114
BfoI RGCGCY 1 cut(s) 1497
BfuI GTATCC 1 cut(s) 2990
BlpI GCTNAGC 1 cut(s) 2833
BmcAI AGTACT 1 cut(s) 2931
Bme1390I CCNGG 8 cut(s) 518, 656, 777, 782, 1491, 1619, 2900, 3365
Bme18I GGWCC 8 cut(s) 355, 859, 898, 1061, 1870, 2123, 2896, 3426
BmeT110I CYCGRG 2 cut(s) 136, 434
BmiI GGNNCC 9 cut(s) 356, 653, 786, 839, 1568, 1777, 2125, 2502, 2514
BmrFI CCNGG 8 cut(s) 518, 656, 777, 782, 1491, 1619, 2900, 3365
BmrI ACTGGG 5 cut(s) 12, 356, 2370, 2610, 2811
BmsI GCATC 8 cut(s) 76, 1057, 1273, 1714, 1827, 2031, 2876, 2986
BmtI GCTAGC 2 cut(s) 263, 2631
BmuI ACTGGG 5 cut(s) 12, 356, 2370, 2610, 2811
BoxI GACNNNNGTC 1 cut(s) 2121
BpiI GAAGAC 3 cut(s) 1694, 1958, 2225
BplI GAGNNNNNCTC 2 cut(s) 3173, 3205
BpmI CTGGAG 4 cut(s) 606, 677, 803, 2066
Bpu1102I GCTNAGC 1 cut(s) 2833
BpuEI CTTGAG 3 cut(s) 810, 1161, 2423
BpuMI CCSGG 3 cut(s) 518, 1619, 3365
BsaBI GATNNNNATC 1 cut(s) 741
BsaJI CCNNGG 7 cut(s) 182, 433, 1130, 1489, 1548, 1703, 1779
BsaWI WCCGGW 4 cut(s) 488, 550, 1872, 3098
BsaXI ACNNNNNCTCC 2 cut(s) 3345, 3375
Bsc4I CCNNNNNNNGG 8 cut(s) 350, 368, 794, 1408, 2303, 2738, 2905, 3101
Bse118I RCCGGY 2 cut(s) 550, 1058
Bse3DI GCAATG 6 cut(s) 288, 419, 475, 999, 2054, 2352
Bse8I GATNNNNATC 1 cut(s) 741
BseAI TCCGGA 1 cut(s) 488
BseBI CCWGG 5 cut(s) 656, 777, 782, 1491, 2900
BseDI CCNNGG 7 cut(s) 182, 433, 1130, 1489, 1548, 1703, 1779
BseJI GATNNNNATC 1 cut(s) 741
BseLI CCNNNNNNNGG 8 cut(s) 350, 368, 794, 1408, 2303, 2738, 2905, 3101
BseMI GCAATG 6 cut(s) 288, 419, 475, 999, 2054, 2352
BseRI GAGGAG 2 cut(s) 125, 2390
BseSI GKGCMC 1 cut(s) 2817
BseYI CCCAGC 2 cut(s) 167, 3142
BsgI GTGCAG 1 cut(s) 3396
Bsh1236I CGCG 2 cut(s) 1281, 1481
BshFI GGCC 6 cut(s) 405, 431, 722, 780, 2198, 3105
BshNI GGYRCC 1 cut(s) 2500
BshTI ACCGGT 1 cut(s) 550
BsiHKAI GWGCWC 3 cut(s) 143, 592, 699
BsiHKCI CYCGRG 2 cut(s) 136, 434
BsiSI CCGG 8 cut(s) 489, 517, 551, 1059, 1619, 1873, 3099, 3364
BslFI GGGAC 6 cut(s) 326, 368, 554, 1124, 2109, 3159
BslI CCNNNNNNNGG 8 cut(s) 350, 368, 794, 1408, 2303, 2738, 2905, 3101
BsmAI GTCTC 4 cut(s) 1087, 1369, 3014, 3279
BsmBI CGTCTC 1 cut(s) 3279
BsmFI GGGAC 6 cut(s) 326, 368, 554, 1124, 2109, 3159
BsmI GAATGC 4 cut(s) 310, 1760, 1762, 2149
BsnI GGCC 6 cut(s) 405, 431, 722, 780, 2198, 3105
BsoBI CYCGRG 2 cut(s) 136, 434
Bsp1286I GDGCHC 5 cut(s) 143, 592, 699, 958, 2817
Bsp13I TCCGGA 1 cut(s) 488
Bsp1407I TGTACA 2 cut(s) 2269, 3352
Bsp1720I GCTNAGC 1 cut(s) 2833
Bsp19I CCATGG 1 cut(s) 1779
BspACI CCGC 2 cut(s) 1481, 2679
BspANI GGCC 6 cut(s) 405, 431, 722, 780, 2198, 3105
BspEI TCCGGA 1 cut(s) 488
BspFNI CGCG 2 cut(s) 1281, 1481
BspHI TCATGA 4 cut(s) 733, 2955, 3012, 3339
BspLI GGNNCC 9 cut(s) 356, 653, 786, 839, 1568, 1777, 2125, 2502, 2514
BspMAI CTGCAG 1 cut(s) 1588
BspOI GCTAGC 2 cut(s) 263, 2631
BspPI GGATC 9 cut(s) 180, 232, 933, 1227, 1332, 1566, 2284, 2423, 3224
BspT107I GGYRCC 1 cut(s) 2500
BsrDI GCAATG 6 cut(s) 288, 419, 475, 999, 2054, 2352
BsrFI RCCGGY 2 cut(s) 550, 1058
BsrGI TGTACA 2 cut(s) 2269, 3352
BssAI RCCGGY 2 cut(s) 550, 1058
BssECI CCNNGG 7 cut(s) 182, 433, 1130, 1489, 1548, 1703, 1779
BssSI CACGAG 1 cut(s) 2800
BssT1I CCWWGG 4 cut(s) 1130, 1548, 1703, 1779
Bst2BI CACGAG 1 cut(s) 2800
Bst2UI CCWGG 5 cut(s) 656, 777, 782, 1491, 2900
Bst6I CTCTTC 2 cut(s) 2681, 3000
BstAPI GCANNNNNTGC 1 cut(s) 640
BstAUI TGTACA 2 cut(s) 2269, 3352
BstC8I GCNNGC 7 cut(s) 257, 261, 2155, 2625, 2629, 2824, 3107
BstDSI CCRYGG 1 cut(s) 1779
BstFNI CGCG 2 cut(s) 1281, 1481
BstH2I RGCGCY 1 cut(s) 1497
BstHHI GCGC 3 cut(s) 1075, 1281, 1496
BstMAI GTCTC 4 cut(s) 1087, 1369, 3014, 3279
BstMWI GCNNNNNNNGC 5 cut(s) 587, 617, 640, 772, 3404
BstNI CCWGG 5 cut(s) 656, 777, 782, 1491, 2900
BstPAI GACNNNNGTC 1 cut(s) 2121
BstSCI CCNGG 8 cut(s) 516, 654, 775, 780, 1489, 1617, 2898, 3363
BstSFI CTRYAG 4 cut(s) 81, 507, 1584, 3114
BstSLI GKGCMC 1 cut(s) 2817
BstUI CGCG 2 cut(s) 1281, 1481
BstV2I GAAGAC 3 cut(s) 1694, 1958, 2225
BstX2I RGATCY 2 cut(s) 925, 1324
BstXI CCANNNNNNTGG 2 cut(s) 248, 3272
BstYI RGATCY 2 cut(s) 925, 1324
BsuI GTATCC 1 cut(s) 2990
BsuRI GGCC 6 cut(s) 405, 431, 722, 780, 2198, 3105
BtgI CCRYGG 1 cut(s) 1779
BtgZI GCGATG 1 cut(s) 1296
BtsI GCAGTG 4 cut(s) 54, 201, 1578, 1860
Cac8I GCNNGC 7 cut(s) 257, 261, 2155, 2625, 2629, 2824, 3107
CaiI CAGNNNCTG 1 cut(s) 167
CciI TCATGA 4 cut(s) 733, 2955, 3012, 3339
CfoI GCGC 3 cut(s) 1075, 1281, 1496
Cfr10I RCCGGY 2 cut(s) 550, 1058
CseI GACGC 1 cut(s) 1487
Csp6I GTAC 9 cut(s) 1248, 1461, 2270, 2545, 2772, 2930, 3164, 3353, 3358
CspAI ACCGGT 1 cut(s) 550
CviQI GTAC 9 cut(s) 1248, 1461, 2270, 2545, 2772, 2930, 3164, 3353, 3358
DraIII CACNNNGTG 2 cut(s) 1434, 2105
Eam1104I CTCTTC 2 cut(s) 2681, 3000
EarI CTCTTC 2 cut(s) 2681, 3000
Ecl136II GAGCTC 3 cut(s) 141, 590, 697
Eco130I CCWWGG 4 cut(s) 1130, 1548, 1703, 1779
Eco24I GRGCYC 4 cut(s) 143, 592, 699, 958
Eco32I GATATC 2 cut(s) 2206, 3225
Eco47I GGWCC 8 cut(s) 355, 859, 898, 1061, 1870, 2123, 2896, 3426
Eco53kI GAGCTC 3 cut(s) 141, 590, 697
Eco57I CTGAAG 1 cut(s) 3315
Eco88I CYCGRG 2 cut(s) 136, 434
EcoICRI GAGCTC 3 cut(s) 141, 590, 697
EcoO109I RGGNCCY 4 cut(s) 404, 430, 898, 3426
EcoRI GAATTC 1 cut(s) 994
EcoRII CCWGG 5 cut(s) 654, 775, 780, 1489, 2898
EcoRV GATATC 2 cut(s) 2206, 3225
EcoT14I CCWWGG 4 cut(s) 1130, 1548, 1703, 1779
EcoT22I ATGCAT 1 cut(s) 1762
EcoT38I GRGCYC 4 cut(s) 143, 592, 699, 958
ErhI CCWWGG 4 cut(s) 1130, 1548, 1703, 1779
Esp3I CGTCTC 1 cut(s) 3279
FalI AAGNNNNNCTT 2 cut(s) 3368, 3400
FaqI GGGAC 6 cut(s) 326, 368, 554, 1124, 2109, 3159
FauI CCCGC 1 cut(s) 1474
FauNDI CATATG 1 cut(s) 1440
FbaI TGATCA 1 cut(s) 742
FriOI GRGCYC 4 cut(s) 143, 592, 699, 958
FspI TGCGCA 1 cut(s) 1074
GlaI GCGC 3 cut(s) 1074, 1280, 1495
GsaI CCCAGC 2 cut(s) 171, 3146
GsuI CTGGAG 4 cut(s) 606, 677, 803, 2066
HaeII RGCGCY 1 cut(s) 1497
HaeIII GGCC 6 cut(s) 405, 431, 722, 780, 2198, 3105
HapII CCGG 8 cut(s) 489, 517, 551, 1059, 1619, 1873, 3099, 3364
HgaI GACGC 1 cut(s) 1487
HhaI GCGC 3 cut(s) 1075, 1281, 1496
Hin6I GCGC 3 cut(s) 1073, 1279, 1494
HinP1I GCGC 3 cut(s) 1073, 1279, 1494
HincII GTYRAC 5 cut(s) 874, 1930, 1959, 2358, 3381
HindII GTYRAC 5 cut(s) 874, 1930, 1959, 2358, 3381
HindIII AAGCTT 3 cut(s) 92, 828, 2451
HpaI GTTAAC 3 cut(s) 1930, 1959, 2358
HpaII CCGG 8 cut(s) 489, 517, 551, 1059, 1619, 1873, 3099, 3364
HphI GGTGA 5 cut(s) 565, 727, 1520, 1874, 2264
HpyAV CCTTC 9 cut(s) 911, 1028, 1034, 1621, 1702, 2243, 3245, 3293, 3394
HpyCH4IV ACGT 1 cut(s) 3288
HpyF10VI GCNNNNNNNGC 5 cut(s) 587, 617, 640, 772, 3404
HpySE526I ACGT 1 cut(s) 3288
HspAI GCGC 3 cut(s) 1073, 1279, 1494
Kpn2I TCCGGA 1 cut(s) 488
Ksp22I TGATCA 1 cut(s) 742
KspAI GTTAAC 3 cut(s) 1930, 1959, 2358
LmnI GCTCC 6 cut(s) 587, 651, 784, 1291, 1695, 2783
LweI GCATC 8 cut(s) 76, 1057, 1273, 1714, 1827, 2031, 2876, 2986
MaeII ACGT 1 cut(s) 3288
MaeIII GTNAC 8 cut(s) 20, 196, 520, 1302, 1394, 1817, 1855, 2596
MfeI CAATTG 3 cut(s) 621, 1983, 2265
MflI RGATCY 2 cut(s) 925, 1324
MhlI GDGCHC 5 cut(s) 143, 592, 699, 958, 2817
MlyI GAGTC 3 cut(s) 122, 1072, 2845
MmeI TCCRAC 3 cut(s) 1194, 2683, 3194
Mph1103I ATGCAT 1 cut(s) 1762
MroI TCCGGA 1 cut(s) 488
MroXI GAANNNNTTC 2 cut(s) 1760, 2511
MslI CAYNNNNRTG 3 cut(s) 738, 1193, 2006
MspA1I CMGCKG 2 cut(s) 1532, 3142
MspI CCGG 8 cut(s) 489, 517, 551, 1059, 1619, 1873, 3099, 3364
MspR9I CCNGG 8 cut(s) 518, 656, 777, 782, 1491, 1619, 2900, 3365
MunI CAATTG 3 cut(s) 621, 1983, 2265
Mva1269I GAATGC 4 cut(s) 310, 1760, 1762, 2149
MvaI CCWGG 5 cut(s) 656, 777, 782, 1491, 2900
MvnI CGCG 2 cut(s) 1281, 1481
MwoI GCNNNNNNNGC 5 cut(s) 587, 617, 640, 772, 3404
NciI CCSGG 3 cut(s) 518, 1619, 3365
NcoI CCATGG 1 cut(s) 1779
NdeI CATATG 1 cut(s) 1440
NheI GCTAGC 2 cut(s) 259, 2627
NlaIV GGNNCC 9 cut(s) 356, 653, 786, 839, 1568, 1777, 2125, 2502, 2514
NmuCI GTSAC 7 cut(s) 20, 196, 1302, 1394, 1817, 1855, 2596
NsbI TGCGCA 1 cut(s) 1074
NsiI ATGCAT 1 cut(s) 1762
OliI CACNNNNGTG 1 cut(s) 2006
PaeR7I CTCGAG 1 cut(s) 136
PagI TCATGA 4 cut(s) 733, 2955, 3012, 3339
PctI GAATGC 4 cut(s) 310, 1760, 1762, 2149
PdmI GAANNNNTTC 2 cut(s) 1760, 2511
PfeI GAWTC 9 cut(s) 1405, 1445, 1800, 2015, 2338, 2443, 2530, 2728, 3244
PflFI GACNNNGTC 1 cut(s) 18
PflMI CCANNNNNTGG 2 cut(s) 368, 2303
PfoI TCCNGGA 1 cut(s) 3363
PinAI ACCGGT 1 cut(s) 550
PleI GAGTC 3 cut(s) 121, 1072, 2845
PpsI GAGTC 3 cut(s) 121, 1072, 2845
PpuMI RGGWCCY 2 cut(s) 898, 3426
PshAI GACNNNNGTC 1 cut(s) 2121
PsiI TTATAA 1 cut(s) 2259
Psp124BI GAGCTC 3 cut(s) 143, 592, 699
Psp5II RGGWCCY 2 cut(s) 898, 3426
Psp6I CCWGG 5 cut(s) 654, 775, 780, 1489, 2898
PspFI CCCAGC 2 cut(s) 167, 3142
PspGI CCWGG 5 cut(s) 654, 775, 780, 1489, 2898
PspN4I GGNNCC 9 cut(s) 356, 653, 786, 839, 1568, 1777, 2125, 2502, 2514
PspPPI RGGWCCY 2 cut(s) 898, 3426
PspXI VCTCGAGB 1 cut(s) 136
PstI CTGCAG 1 cut(s) 1588
PstNI CAGNNNCTG 1 cut(s) 167
PsuI RGATCY 2 cut(s) 925, 1324
PsyI GACNNNGTC 1 cut(s) 18
PvuII CAGCTG 2 cut(s) 1532, 3142
RsaI GTAC 9 cut(s) 1249, 1462, 2271, 2546, 2773, 2931, 3165, 3354, 3359
RsaNI GTAC 9 cut(s) 1248, 1461, 2270, 2545, 2772, 2930, 3164, 3353, 3358
RseI CAYNNNNRTG 3 cut(s) 738, 1193, 2006
SacI GAGCTC 3 cut(s) 143, 592, 699
ScaI AGTACT 1 cut(s) 2931
SchI GAGTC 3 cut(s) 122, 1072, 2845
ScrFI CCNGG 8 cut(s) 518, 656, 777, 782, 1491, 1619, 2900, 3365
SduI GDGCHC 5 cut(s) 143, 592, 699, 958, 2817
SfaNI GCATC 8 cut(s) 76, 1057, 1273, 1714, 1827, 2031, 2876, 2986
SfcI CTRYAG 4 cut(s) 81, 507, 1584, 3114
Sfr274I CTCGAG 1 cut(s) 136
SinI GGWCC 8 cut(s) 355, 859, 898, 1061, 1870, 2123, 2896, 3426
SlaI CTCGAG 1 cut(s) 136
SmiMI CAYNNNNRTG 3 cut(s) 738, 1193, 2006
SmlI CTYRAG 4 cut(s) 136, 825, 1176, 2438
SmoI CTYRAG 4 cut(s) 136, 825, 1176, 2438
SpeI ACTAGT 1 cut(s) 3328
SsiI CCGC 2 cut(s) 1481, 2679
SspI AATATT 2 cut(s) 1318, 2301
SstI GAGCTC 3 cut(s) 143, 592, 699
StyD4I CCNGG 8 cut(s) 516, 654, 775, 780, 1489, 1617, 2898, 3363
StyI CCWWGG 4 cut(s) 1130, 1548, 1703, 1779
TaiI ACGT 1 cut(s) 3291
TaqI TCGA 4 cut(s) 137, 2088, 2533, 2766
TatI WGTACW 5 cut(s) 1460, 2269, 2544, 2929, 3352
TfiI GAWTC 9 cut(s) 1405, 1445, 1800, 2015, 2338, 2443, 2530, 2728, 3244
TseFI GTSAC 7 cut(s) 20, 196, 1302, 1394, 1817, 1855, 2596
Tsp45I GTSAC 7 cut(s) 20, 196, 1302, 1394, 1817, 1855, 2596
TspGWI ACGGA 2 cut(s) 851, 2274
Tth111I GACNNNGTC 1 cut(s) 18
Van91I CCANNNNNTGG 2 cut(s) 368, 2303
VpaK11BI GGWCC 8 cut(s) 355, 859, 898, 1061, 1870, 2123, 2896, 3426
XapI RAATTY 5 cut(s) 728, 994, 1947, 1993, 2315
XcmI CCANNNNNNNNNTGG 2 cut(s) 365, 2207
XhoI CTCGAG 1 cut(s) 136
XmnI GAANNNNTTC 2 cut(s) 1760, 2511
ZrmI AGTACT 1 cut(s) 2931
Zsp2I ATGCAT 1 cut(s) 1762
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.