Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0009869

Physical Location & Seq

Genomic Coordinates Reverse (-)

29956094 .. 29957230

Gene Structure 1137 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1222600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1137 bp

ATGCCTCCGCTCTTCTCCTGCGCCGCCAGGCACATGGCCCTCGCTCTCTTCTTCTTCCTCCTCCTTTCTCCACAAATCTCGCTCGTCAGAGCTCAGCCCGGACCGGACCGAGCCGACCCTTACCGCTACCAGAGGTTCAGCCCGTCGATGGCCATCATCATCGTTGTACTAATCGCCGCCCTCTTCCTCATGGCCTTCTTCTCAATCTACATCCGCCACTGCTCCGAAGCCAACCAACCCGGCAGCGTCCGCCCCGGAGGGCCCCACACCGGCCGATCCCGACGCGGCGCAGCCTCCCGCGGCCTCGACGCCGCCGTCCTCGACACCTTCCCCACCCTCGACTACGCCGAAATCAAAGGGCTTAAAATCGGGAAAGAGGCGCTCGAGTGCGCCGTCTGCTTAAACGAATTCGAGGACGACGAAACGTTGCGTTTGATCCCCAAATGCGACCACGTGTTCCACCCCGAGTGCATCGACGAGTGGCTGGCGTCACACACCACCTGTCCCGTGTGCCGGGCCAACCTCGTTCCTCAGCCCGGCGACTCGGCGCCACAACTCACTCAGTTGGGATCGGCGCAGCAGGCGGGCGACGTCGAAGCTCAAAACGACGCCGTTAACTCGGCGGCGGAGAGAGATGACCAGGTAATGCTAGAACGACCACAACAAGAACCTGAGCTGTTGAACTTAAACCAGACGCTGAATCGGAACCGTACACGCGGATCGAGATCAAGCAGGTTGCTTAGATTGTTCCCGCGGTCGCACTCGACCGGACACTCTCTTGTCCAACCGGGAGAAGACACTGAGCGGTTCACCTTGAGACTACCGGTTGAAGTGAGAAAACAGATCATGAATCGGAAGCTGAACCGGTCCACTAGCTTGTTGGTCTTGCCCCGAGAAGGGAGTTCGAGGCGTGGTCCTCGAACCGGCGAGAGTGGCAGTACTAATAGAGTGAGGAGCTACAGAAGGCTCGAGCCATTGGACCGGCAATTCAAATCGGACCGGTGGGTGTTCTCCAAAACGCCGACGTTTCTCACGAGGATGTCGAGTCTCTTGTCGCCAAAGGTGGCGGTAAGTGACGGCGAGAAGCCGACCGAGCCAGCGATGTCGAGGAGTAGCTCCGCTCGGCCTCCGGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002376 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009617 GO:0009719 GO:0009725 GO:0009737 GO:0009814 GO:0009816 GO:0009987 GO:0009991 GO:0010033 GO:0010200 GO:0010243 GO:0010498 GO:0016020 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0031667 GO:0031668 GO:0031669 GO:0032446 GO:0033993 GO:0036211 GO:0042221 GO:0042493 GO:0042742 GO:0043161 GO:0043170 GO:0043207 GO:0043412 GO:0043562 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044464 GO:0045087 GO:0050896 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0061630 GO:0061659 GO:0070647 GO:0071496 GO:0071704 GO:0071944 GO:0097305 GO:0098542 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

379

Amino Acids

42.14

Weight (kDa)

9.42

Isoelectric Point (pI)

56.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	129 - 172	2.6e-14	Ring finger domain
zf-RING_11	PF17123	129 - 158	8.1e-10	RING-like zinc finger
zf-C3HC4_2	PF13923	129 - 171	1.1e-08	Zinc finger, C3HC4 type (RING finger)
zf-RING_5	PF14634	129 - 172	9.9e-06	zinc-RING finger domain
zf-C3HC4	PF00097	130 - 171	1.4e-06	Zinc finger, C3HC4 type (RING finger)
zf-rbx1	PF12678	131 - 172	5.3e-08	RING-H2 zinc finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009869)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05200 AT5G27420
fragaria_vesca	FvH4_6g05450
malus_domestica	MD04G1208600.v1.1 MD12G1222600.v1.1
prunus_persica	Prupe.6G329500_v2.0.a1
pyrus_communis	pycom04g18470 pycom12g20880
rosa_chinensis	RchiOBHm_Chr3g0454221
rosa_laevigata	RLG00000025475
rosa_multiflora	Rmu_sc0007628.1_g000006 Rmu_sc0026184.1_g000001 Rmu_sc0026185.1_g000001
rosa_roxburghii	Rroxscaffold_165G00437000 Rroxscaffold_6G00425360
rosa_rugosa	Rorug02G0653500
rosa_samantha	Rh3AG056800 Rh3BG058200 Rh3CG057400 Rh3DG058700
rosa_wichuraiana	Rw3G004370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	314
AatII	GACGTC	1 cut(s)	594
Acc36I	ACCTGC	1 cut(s)	723
AccB1I	GGYRCC	1 cut(s)	547
AccBSI	CCGCTC	3 cut(s)	10, 805, 1121
AccII	CGCG	4 cut(s)	285, 300, 717, 754
AclI	AACGTT	1 cut(s)	425
AclWI	GGATC	4 cut(s)	270, 430, 577, 727
AcoI	YGGCCR	2 cut(s)	150, 271
AcsI	RAATTY	1 cut(s)	407
AcvI	CACGTG	1 cut(s)	454
AcyI	GRCGYC	5 cut(s)	309, 488, 548, 591, 609
AfaI	GTAC	3 cut(s)	168, 712, 940
AfiI	CCNNNNNNNGG	7 cut(s)	148, 269, 513, 536, 896, 897, 923
AflIII	ACRYGT	1 cut(s)	453
AgeI	ACCGGT	3 cut(s)	823, 864, 999
AgsI	TTSAA	3 cut(s)	682, 830, 991
AjnI	CCWGG	2 cut(s)	26, 639
AluBI	AGCT	7 cut(s)	92, 599, 676, 859, 876, 957, 1116
AluI	AGCT	7 cut(s)	92, 599, 676, 859, 876, 957, 1116
Alw21I	GWGCWC	1 cut(s)	94
Alw26I	GTCTC	2 cut(s)	811, 1052
AlwI	GGATC	4 cut(s)	270, 430, 577, 727
AlwNI	CAGNNNCTG	1 cut(s)	697
Ama87I	CYCGRG	4 cut(s)	383, 464, 891, 968
AoxI	GGCC	8 cut(s)	36, 150, 192, 260, 271, 301, 516, 1124
ApaI	GGGCCC	1 cut(s)	264
ApeKI	GCWGC	3 cut(s)	243, 290, 577
ApoI	RAATTY	1 cut(s)	407
AsiGI	ACCGGT	3 cut(s)	823, 864, 999
AspLEI	GCGC	6 cut(s)	23, 290, 382, 392, 550, 577
AsuC2I	CCSGG	6 cut(s)	99, 240, 255, 515, 537, 789
AsuHPI	GGTGA	1 cut(s)	802
AvaI	CYCGRG	4 cut(s)	383, 464, 891, 968
AvaII	GGWCC	6 cut(s)	101, 106, 867, 914, 979, 997
BaeGI	GKGCMC	1 cut(s)	264
BalI	TGGCCA	1 cut(s)	152
BanI	GGYRCC	1 cut(s)	547
BanII	GRGCYC	2 cut(s)	94, 264
BauI	CACGAG	1 cut(s)	1033
BbrPI	CACGTG	1 cut(s)	454
BbsI	GAAGAC	1 cut(s)	801
Bbv12I	GWGCWC	1 cut(s)	94
BbvCI	CCTCAGC	1 cut(s)	531
BbvI	GCAGC	3 cut(s)	255, 302, 589
BccI	CCATC	2 cut(s)	142, 161
BceAI	ACGGC	4 cut(s)	299, 377, 596, 1093
BciT130I	CCWGG	2 cut(s)	28, 641
BcnI	CCSGG	6 cut(s)	99, 240, 255, 515, 537, 789
BcoDI	GTCTC	2 cut(s)	811, 1052
BfaI	CTAG	2 cut(s)	650, 873
BfmI	CTRYAG	1 cut(s)	958
BfoI	RGCGCY	2 cut(s)	383, 551
BfuAI	ACCTGC	1 cut(s)	723
BisI	GCNGC	9 cut(s)	24, 177, 244, 286, 291, 301, 312, 578, 624
BlpI	GCTNAGC	1 cut(s)	93
BlsI	GCNGC	9 cut(s)	25, 178, 245, 287, 292, 302, 313, 579, 625
BmcAI	AGTACT	1 cut(s)	940
Bme1390I	CCNGG	8 cut(s)	28, 99, 240, 255, 515, 537, 641, 789
Bme18I	GGWCC	6 cut(s)	101, 106, 867, 914, 979, 997
BmeT110I	CYCGRG	4 cut(s)	383, 464, 891, 968
BmiI	GGNNCC	4 cut(s)	262, 263, 549, 707
BmrFI	CCNGG	8 cut(s)	28, 99, 240, 255, 515, 537, 641, 789
BmsI	GCATC	1 cut(s)	480
BpiI	GAAGAC	1 cut(s)	801
Bpu10I	CCTNAGC	2 cut(s)	531, 672
Bpu1102I	GCTNAGC	1 cut(s)	93
BpuEI	CTTGAG	1 cut(s)	835
BpuMI	CCSGG	6 cut(s)	99, 240, 255, 515, 537, 789
BsaAI	YACGTR	1 cut(s)	454
BsaBI	GATNNNNATC	2 cut(s)	152, 724
BsaHI	GRCGYC	5 cut(s)	309, 488, 548, 591, 609
BsaJI	CCNNGG	3 cut(s)	253, 298, 752
BsaWI	WCCGGW	6 cut(s)	103, 767, 823, 864, 999, 1129
Bsc4I	CCNNNNNNNGG	7 cut(s)	148, 269, 513, 536, 896, 897, 923
Bse118I	RCCGGY	6 cut(s)	269, 823, 864, 923, 981, 999
Bse8I	GATNNNNATC	2 cut(s)	152, 724
BseBI	CCWGG	2 cut(s)	28, 641
BseDI	CCNNGG	3 cut(s)	253, 298, 752
BseGI	GGATG	2 cut(s)	210, 1044
BseJI	GATNNNNATC	2 cut(s)	152, 724
BseLI	CCNNNNNNNGG	7 cut(s)	148, 269, 513, 536, 896, 897, 923
BseMII	CTCAG	5 cut(s)	107, 545, 575, 663, 792
BseRI	GAGGAG	3 cut(s)	50, 967, 1123
BseSI	GKGCMC	1 cut(s)	264
BseX3I	CGGCCG	1 cut(s)	271
BseXI	GCAGC	3 cut(s)	255, 302, 589
Bsh1236I	CGCG	4 cut(s)	285, 300, 717, 754
Bsh1285I	CGRYCG	4 cut(s)	274, 758, 768, 1092
BshFI	GGCC	8 cut(s)	38, 152, 194, 262, 273, 303, 518, 1126
BshNI	GGYRCC	1 cut(s)	547
BshTI	ACCGGT	3 cut(s)	823, 864, 999
BsiEI	CGRYCG	4 cut(s)	274, 758, 768, 1092
BsiHKAI	GWGCWC	1 cut(s)	94
BsiHKCI	CYCGRG	4 cut(s)	383, 464, 891, 968
BslFI	GGGAC	1 cut(s)	489
BslI	CCNNNNNNNGG	7 cut(s)	148, 269, 513, 536, 896, 897, 923
BsmAI	GTCTC	2 cut(s)	811, 1052
BsmFI	GGGAC	1 cut(s)	489
BsnI	GGCC	8 cut(s)	38, 152, 194, 262, 273, 303, 518, 1126
BsoBI	CYCGRG	4 cut(s)	383, 464, 891, 968
Bsp120I	GGGCCC	1 cut(s)	260
Bsp1286I	GDGCHC	2 cut(s)	94, 264
Bsp143I	GATC	6 cut(s)	275, 435, 569, 719, 725, 843
Bsp1720I	GCTNAGC	1 cut(s)	93
BspANI	GGCC	8 cut(s)	38, 152, 194, 262, 273, 303, 518, 1126
BspCNI	CTCAG	5 cut(s)	106, 544, 574, 664, 793
BspFNI	CGCG	4 cut(s)	285, 300, 717, 754
BspHI	TCATGA	1 cut(s)	846
BspLI	GGNNCC	4 cut(s)	262, 263, 549, 707
BspMI	ACCTGC	1 cut(s)	723
BspPI	GGATC	4 cut(s)	270, 430, 577, 727
BspQI	GCTCTTC	1 cut(s)	17
BspT107I	GGYRCC	1 cut(s)	547
BsrBI	CCGCTC	3 cut(s)	10, 805, 1121
BsrFI	RCCGGY	6 cut(s)	269, 823, 864, 923, 981, 999
BssAI	RCCGGY	6 cut(s)	269, 823, 864, 923, 981, 999
BssECI	CCNNGG	3 cut(s)	253, 298, 752
BssMI	GATC	6 cut(s)	275, 435, 569, 719, 725, 843
BssNI	GRCGYC	5 cut(s)	309, 488, 548, 591, 609
BssSI	CACGAG	1 cut(s)	1033
Bst2BI	CACGAG	1 cut(s)	1033
Bst2UI	CCWGG	2 cut(s)	28, 641
Bst4CI	ACNGT	1 cut(s)	710
Bst6I	CTCTTC	3 cut(s)	17, 53, 188
BstACI	GRCGYC	5 cut(s)	309, 488, 548, 591, 609
BstBAI	YACGTR	1 cut(s)	454
BstC8I	GCNNGC	4 cut(s)	486, 582, 586, 1098
BstDEI	CTNAG	6 cut(s)	93, 531, 561, 672, 740, 801
BstDSI	CCRYGG	2 cut(s)	298, 752
BstF5I	GGATG	2 cut(s)	210, 1044
BstFNI	CGCG	4 cut(s)	285, 300, 717, 754
BstH2I	RGCGCY	2 cut(s)	383, 551
BstHHI	GCGC	6 cut(s)	23, 290, 382, 392, 550, 577
BstKTI	GATC	6 cut(s)	278, 438, 572, 722, 728, 846
BstMAI	GTCTC	2 cut(s)	811, 1052
BstMBI	GATC	6 cut(s)	275, 435, 569, 719, 725, 843
BstMCI	CGRYCG	4 cut(s)	274, 758, 768, 1092
BstMWI	GCNNNNNNNGC	5 cut(s)	249, 396, 581, 933, 1093
BstNI	CCWGG	2 cut(s)	28, 641
BstSCI	CCNGG	8 cut(s)	26, 97, 238, 253, 513, 535, 639, 787
BstSFI	CTRYAG	1 cut(s)	958
BstSLI	GKGCMC	1 cut(s)	264
BstUI	CGCG	4 cut(s)	285, 300, 717, 754
BstV1I	GCAGC	3 cut(s)	255, 302, 589
BstV2I	GAAGAC	1 cut(s)	801
BstXI	CCANNNNNNTGG	1 cut(s)	34
BstZI	CGGCCG	1 cut(s)	271
BsuRI	GGCC	8 cut(s)	38, 152, 194, 262, 273, 303, 518, 1126
BtgI	CCRYGG	2 cut(s)	298, 752
BtgZI	GCGATG	1 cut(s)	1115
BtsCI	GGATG	2 cut(s)	210, 1044
BtsI	GCAGTG	1 cut(s)	217
BtsIMutI	CAGTG	2 cut(s)	217, 798
BveI	ACCTGC	1 cut(s)	723
Cac8I	GCNNGC	4 cut(s)	486, 582, 586, 1098
CaiI	CAGNNNCTG	1 cut(s)	697
CciI	TCATGA	1 cut(s)	846
CfoI	GCGC	6 cut(s)	23, 290, 382, 392, 550, 577
Cfr10I	RCCGGY	6 cut(s)	269, 823, 864, 923, 981, 999
Cfr42I	CCGCGG	2 cut(s)	301, 755
CpoI	CGGWCCG	3 cut(s)	101, 106, 997
CseI	GACGC	6 cut(s)	235, 291, 317, 477, 617, 703
CsiI	ACCWGGT	1 cut(s)	639
Csp6I	GTAC	3 cut(s)	167, 711, 939
CspAI	ACCGGT	3 cut(s)	823, 864, 999
CspI	CGGWCCG	3 cut(s)	101, 106, 997
CviAII	CATG	3 cut(s)	34, 190, 847
CviQI	GTAC	3 cut(s)	167, 711, 939
DdeI	CTNAG	6 cut(s)	93, 531, 561, 672, 740, 801
DinI	GGCGCC	1 cut(s)	549
DpnI	GATC	6 cut(s)	277, 437, 571, 721, 727, 845
DpnII	GATC	6 cut(s)	275, 435, 569, 719, 725, 843
DrdI	GACNNNNNNGTC	1 cut(s)	314
DseDI	GACNNNNNNGTC	1 cut(s)	314
EaeI	YGGCCR	2 cut(s)	150, 271
EagI	CGGCCG	1 cut(s)	271
Eam1104I	CTCTTC	3 cut(s)	17, 53, 188
EarI	CTCTTC	3 cut(s)	17, 53, 188
EciI	GGCGGA	3 cut(s)	203, 239, 641
Ecl136II	GAGCTC	1 cut(s)	92
EclXI	CGGCCG	1 cut(s)	271
Eco24I	GRGCYC	2 cut(s)	94, 264
Eco47I	GGWCC	6 cut(s)	101, 106, 867, 914, 979, 997
Eco52I	CGGCCG	1 cut(s)	271
Eco53kI	GAGCTC	1 cut(s)	92
Eco72I	CACGTG	1 cut(s)	454
Eco88I	CYCGRG	4 cut(s)	383, 464, 891, 968
EcoICRI	GAGCTC	1 cut(s)	92
EcoO109I	RGGNCCY	2 cut(s)	260, 261
EcoRI	GAATTC	1 cut(s)	407
EcoRII	CCWGG	2 cut(s)	26, 639
EcoT38I	GRGCYC	2 cut(s)	94, 264
EgeI	GGCGCC	1 cut(s)	549
EheI	GGCGCC	1 cut(s)	549
FaeI	CATG	3 cut(s)	37, 193, 850
FaiI	YATR	3 cut(s)	35, 191, 848
FaqI	GGGAC	1 cut(s)	489
FatI	CATG	3 cut(s)	33, 189, 846
FauI	CCCGC	3 cut(s)	305, 577, 759
Fnu4HI	GCNGC	9 cut(s)	24, 177, 244, 286, 291, 301, 312, 578, 624
FokI	GGATG	2 cut(s)	197, 1051
FriOI	GRGCYC	2 cut(s)	94, 264
Fsp4HI	GCNGC	9 cut(s)	24, 177, 244, 286, 291, 301, 312, 578, 624
FspBI	CTAG	2 cut(s)	650, 873
GlaI	GCGC	6 cut(s)	22, 289, 381, 391, 549, 576
GluI	GCNGC	9 cut(s)	24, 177, 244, 286, 291, 301, 312, 578, 624
HaeII	RGCGCY	2 cut(s)	383, 551
HaeIII	GGCC	8 cut(s)	38, 152, 194, 262, 273, 303, 518, 1126
HgaI	GACGC	6 cut(s)	235, 291, 317, 477, 617, 703
HhaI	GCGC	6 cut(s)	23, 290, 382, 392, 550, 577
Hin1I	GRCGYC	5 cut(s)	309, 488, 548, 591, 609
Hin1II	CATG	3 cut(s)	37, 193, 850
Hin6I	GCGC	6 cut(s)	21, 288, 380, 390, 548, 575
HinP1I	GCGC	6 cut(s)	21, 288, 380, 390, 548, 575
HincII	GTYRAC	1 cut(s)	616
HindII	GTYRAC	1 cut(s)	616
HinfI	GANTC	4 cut(s)	542, 700, 850, 1045
HpaI	GTTAAC	1 cut(s)	616
HphI	GGTGA	1 cut(s)	802
Hpy166II	GTNNAC	4 cut(s)	616, 713, 810, 870
Hpy188I	TCNGA	5 cut(s)	89, 226, 705, 855, 997
Hpy188III	TCNNGA	5 cut(s)	279, 370, 723, 847, 1033
Hpy8I	GTNNAC	4 cut(s)	616, 713, 810, 870
Hpy99I	CGWCG	9 cut(s)	148, 285, 311, 422, 479, 593, 596, 611, 1027
HpyAV	CCTTC	4 cut(s)	205, 337, 890, 957
HpyCH4III	ACNGT	1 cut(s)	710
HpyCH4IV	ACGT	4 cut(s)	425, 453, 591, 1025
HpyCH4V	TGCA	1 cut(s)	471
HpyF10VI	GCNNNNNNNGC	5 cut(s)	249, 396, 581, 933, 1093
HpyF3I	CTNAG	6 cut(s)	93, 531, 561, 672, 740, 801
HpySE526I	ACGT	4 cut(s)	425, 453, 591, 1025
Hsp92I	GRCGYC	5 cut(s)	309, 488, 548, 591, 609
Hsp92II	CATG	3 cut(s)	37, 193, 850
HspAI	GCGC	6 cut(s)	21, 288, 380, 390, 548, 575
KasI	GGCGCC	1 cut(s)	547
KspAI	GTTAAC	1 cut(s)	616
KspI	CCGCGG	2 cut(s)	301, 755
Kzo9I	GATC	6 cut(s)	275, 435, 569, 719, 725, 843
LguI	GCTCTTC	1 cut(s)	17
LmnI	GCTCC	3 cut(s)	227, 954, 1121
Lsp1109I	GCAGC	3 cut(s)	255, 302, 589
LweI	GCATC	1 cut(s)	480
MabI	ACCWGGT	1 cut(s)	639
MaeI	CTAG	2 cut(s)	650, 873
MaeII	ACGT	4 cut(s)	425, 453, 591, 1025
MaeIII	GTNAC	2 cut(s)	489, 1073
MalI	GATC	6 cut(s)	277, 437, 571, 721, 727, 845
MbiI	CCGCTC	3 cut(s)	10, 805, 1121
MboI	GATC	6 cut(s)	275, 435, 569, 719, 725, 843
MboII	GAAGA	7 cut(s)	4, 40, 43, 46, 175, 190, 806
MhlI	GDGCHC	2 cut(s)	94, 264
MlsI	TGGCCA	1 cut(s)	152
MluCI	AATT	2 cut(s)	407, 986
MluNI	TGGCCA	1 cut(s)	152
Mly113I	GGCGCC	1 cut(s)	548
MlyI	GAGTC	2 cut(s)	536, 1054
MmeI	TCCRAC	1 cut(s)	808
Mox20I	TGGCCA	1 cut(s)	152
MscI	TGGCCA	1 cut(s)	152
MseI	TTAA	5 cut(s)	363, 401, 615, 686, 1135
MslI	CAYNNNNRTG	1 cut(s)	1037
Msp20I	TGGCCA	1 cut(s)	152
MspA1I	CMGCKG	2 cut(s)	300, 754
MspR9I	CCNGG	8 cut(s)	28, 99, 240, 255, 515, 537, 641, 789
MvaI	CCWGG	2 cut(s)	28, 641
MvnI	CGCG	4 cut(s)	285, 300, 717, 754
MwoI	GCNNNNNNNGC	5 cut(s)	249, 396, 581, 933, 1093
NarI	GGCGCC	1 cut(s)	548
NciI	CCSGG	6 cut(s)	99, 240, 255, 515, 537, 789
NdeII	GATC	6 cut(s)	275, 435, 569, 719, 725, 843
NlaIII	CATG	3 cut(s)	37, 193, 850
NlaIV	GGNNCC	4 cut(s)	262, 263, 549, 707
NmeAIII	GCCGAG	3 cut(s)	524, 599, 1102
NmuCI	GTSAC	2 cut(s)	489, 1073
PaeR7I	CTCGAG	2 cut(s)	383, 968
PagI	TCATGA	1 cut(s)	846
PciSI	GCTCTTC	1 cut(s)	17
PcsI	WCGNNNNNNNCGW	6 cut(s)	312, 345, 390, 417, 1031, 1040
PfeI	GAWTC	2 cut(s)	700, 850
PinAI	ACCGGT	3 cut(s)	823, 864, 999
PkrI	GCNGC	9 cut(s)	25, 178, 245, 287, 292, 302, 313, 579, 625
PleI	GAGTC	2 cut(s)	536, 1053
PluTI	GGCGCC	1 cut(s)	551
PmaCI	CACGTG	1 cut(s)	454
PmlI	CACGTG	1 cut(s)	454
PpsI	GAGTC	2 cut(s)	536, 1053
Ppu21I	YACGTR	1 cut(s)	454
Psp124BI	GAGCTC	1 cut(s)	94
Psp1406I	AACGTT	1 cut(s)	425
Psp6I	CCWGG	2 cut(s)	26, 639
PspCI	CACGTG	1 cut(s)	454
PspGI	CCWGG	2 cut(s)	26, 639
PspN4I	GGNNCC	4 cut(s)	262, 263, 549, 707
PspOMI	GGGCCC	1 cut(s)	260
PspXI	VCTCGAGB	2 cut(s)	383, 968
PstNI	CAGNNNCTG	1 cut(s)	697
RsaI	GTAC	3 cut(s)	168, 712, 940
RsaNI	GTAC	3 cut(s)	167, 711, 939
RseI	CAYNNNNRTG	1 cut(s)	1037
Rsr2I	CGGWCCG	3 cut(s)	101, 106, 997
RsrII	CGGWCCG	3 cut(s)	101, 106, 997
SacI	GAGCTC	1 cut(s)	94
SacII	CCGCGG	2 cut(s)	301, 755
SapI	GCTCTTC	1 cut(s)	17
SaqAI	TTAA	5 cut(s)	363, 401, 615, 686, 1135
SatI	GCNGC	9 cut(s)	24, 177, 244, 286, 291, 301, 312, 578, 624
Sau3AI	GATC	6 cut(s)	275, 435, 569, 719, 725, 843
ScaI	AGTACT	1 cut(s)	940
SchI	GAGTC	2 cut(s)	536, 1054
ScrFI	CCNGG	8 cut(s)	28, 99, 240, 255, 515, 537, 641, 789
SduI	GDGCHC	2 cut(s)	94, 264
SexAI	ACCWGGT	1 cut(s)	639
SfaNI	GCATC	1 cut(s)	480
SfcI	CTRYAG	1 cut(s)	958
SfoI	GGCGCC	1 cut(s)	549
Sfr274I	CTCGAG	2 cut(s)	383, 968
Sfr303I	CCGCGG	2 cut(s)	301, 755
SgrBI	CCGCGG	2 cut(s)	301, 755
SinI	GGWCC	6 cut(s)	101, 106, 867, 914, 979, 997
SlaI	CTCGAG	2 cut(s)	383, 968
SmiMI	CAYNNNNRTG	1 cut(s)	1037
SmlI	CTYRAG	3 cut(s)	383, 814, 968
SmoI	CTYRAG	3 cut(s)	383, 814, 968
Sse9I	AATT	2 cut(s)	407, 986
SspDI	GGCGCC	1 cut(s)	547
SspMI	CTAG	2 cut(s)	650, 873
SstI	GAGCTC	1 cut(s)	94
StyD4I	CCNGG	8 cut(s)	26, 97, 238, 253, 513, 535, 639, 787
TaaI	ACNGT	1 cut(s)	710
TaiI	ACGT	4 cut(s)	428, 456, 594, 1028
TaqII	GACCGA	2 cut(s)	123, 1106
TasI	AATT	2 cut(s)	407, 986
TatI	WGTACW	2 cut(s)	166, 938
TauI	GCSGC	6 cut(s)	26, 179, 288, 303, 314, 626
TfiI	GAWTC	2 cut(s)	700, 850
Tru1I	TTAA	5 cut(s)	363, 401, 615, 686, 1135
Tru9I	TTAA	5 cut(s)	363, 401, 615, 686, 1135
TscAI	CASTG	2 cut(s)	224, 805
TseFI	GTSAC	2 cut(s)	489, 1073
TseI	GCWGC	3 cut(s)	243, 290, 577
Tsp45I	GTSAC	2 cut(s)	489, 1073
TspDTI	ATGAA	1 cut(s)	863
TspRI	CASTG	2 cut(s)	224, 805
VpaK11BI	GGWCC	6 cut(s)	101, 106, 867, 914, 979, 997
XapI	RAATTY	1 cut(s)	407
XcmI	CCANNNNNNNNNTGG	1 cut(s)	877
XhoI	CTCGAG	2 cut(s)	383, 968
XspI	CTAG	2 cut(s)	650, 873
ZraI	GACGTC	1 cut(s)	592
ZrmI	AGTACT	1 cut(s)	940

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.