Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr4

Orthogroup ID

OG0009869

Physical Location & Seq

Genomic Coordinates Reverse (-)

20470819 .. 20472459

Gene Structure 1641 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom04g18470.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1455 bp

ATGGCCGAGCAACGCAAGTCGCCGCGGAACGCCACCCATGACCAGCCGCTGACATCACCGCAACCCCCTACCGTTCCATCGCGAGAAAAGCCAGAGAAAGTCCCGGGACTGCGGGTCCCACTCCCGTCCCCATGGCTTGATAGTCCTCGTTTCCCTTCTTCGTCTTCTGCTCTTGTATTCCTTCGTTCCACAAGACACCTTCCCCGACGTTACTTCTCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCGTTTCATTTATAAACCTCCTCCCCCCTTCCTCTCTCACTCTCCTTCTATATCCTCCTTTTCCAGCAATCCTCCCCGATACCACAACAACATGGCTCCGCTCTTATCCTGCGCCGCCAAGCATGTGGTCCTCGCTCTCTTCCTCCTCTTCCTCCTCTCACCGGTTGGAGCCCAGCCCAGTCCGGACGGGTCCGACCCCTACCAGTACCAGCGGTTCAGCCCGCCGTTGGCCATCATCATCGCCGTACTGATCGCCGCCCTCTTCCTCATGGTTTTCTTCTCAGTCTACGTCCGCCGCTGCTCCGACACCTACCAAGCCGACAGCGTCCGCGCCGGCGCCTCCCACACCGGCCGATCCCGACGAGGCGCAGCCTCACGCGGTCTCGACACCGCCGTCCTCGACACCTTCCCCACGCTTGACTACGCCGAGATCAAGGGGCTTAAAATAGGGAAAGAGGCGCTCGAGTGCGCCGTCTGCTTAAACGAATTCGAGGACGACGAAATGCTGCGTTTGATCCCCAAATGCGACCACGTGTTCCACCCTGACTGCATCGACGTGTGGCTGATGTCGCACACCACGTGTCCCGTGTGCCGGGCCAACCTCGTTCCGCAGCCCGGCGACTCGGCACCACAACTCACTGACTTGGGATCCGAGCAGCAGGAGGGCGACGTCGAAGCTCAAAACGACGCCGTAGACGCGGAACGGGAGAGAGATGACCTGGGAATGCTAGCACAGCAAGAGCCTGAGGTGTTGAACATAAACCAGACGCTCAATCGGAACCGTACTCGTGGATCGAGATCGAGCAGGCTGCGGAGATTGTTCTCGCGCTCACACTCGACCGGACACTCTGCGGTCCAACCGGGAGAAGACACTGAGCGGTTCACCTTGAGGTTACCGGTTGAAGTCAGGAAACAGATTATGAACCGGAAGCTGAACCGGTCCACTAGCTTATTGGTCTTGCCCCGAGAAGGGAGTTCGAGGCGTGGGCCCCGAACCGGTGAGAGTAGCAGTATTAATAGAGTGAGGAGCTACAGAAGGCTCGAGCCGTTGGACCGGCAATTTAGATCGGACCGGTGGGTGTTCAACAAAACGCCACCATTTCTCACAAGGATGTCAAGTCGTTCGTCGCCGAAAGTGGCGGCAATTGAAGGTGAGACACCGGCCGTGCCGGAAATGTCTAGGAGCAGTTCGGCTCGGCCTCCGGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002376 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009617 GO:0009719 GO:0009725 GO:0009737 GO:0009814 GO:0009816 GO:0009987 GO:0009991 GO:0010033 GO:0010200 GO:0010243 GO:0010498 GO:0016020 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0031667 GO:0031668 GO:0031669 GO:0032446 GO:0033993 GO:0036211 GO:0042221 GO:0042493 GO:0042742 GO:0043161 GO:0043170 GO:0043207 GO:0043412 GO:0043562 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044464 GO:0045087 GO:0050896 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0061630 GO:0061659 GO:0070647 GO:0071496 GO:0071704 GO:0071944 GO:0097305 GO:0098542 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

485

Amino Acids

54.08

Weight (kDa)

9.68

Isoelectric Point (pI)

73.4

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009869)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05200 AT5G27420
fragaria_vesca	FvH4_6g05450
malus_domestica	MD04G1208600.v1.1 MD12G1222600.v1.1
prunus_persica	Prupe.6G329500_v2.0.a1
pyrus_communis	pycom04g18470 pycom12g20880
rosa_chinensis	RchiOBHm_Chr3g0454221
rosa_laevigata	RLG00000025475
rosa_multiflora	Rmu_sc0007628.1_g000006 Rmu_sc0026184.1_g000001 Rmu_sc0026185.1_g000001
rosa_roxburghii	Rroxscaffold_165G00437000 Rroxscaffold_6G00425360
rosa_rugosa	Rorug02G0653500
rosa_samantha	Rh3AG056800 Rh3BG058200 Rh3CG057400 Rh3DG058700
rosa_wichuraiana	Rw3G004370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	258
AasI	GACNNNNNNGTC	1 cut(s)	638
AatII	GACGTC	1 cut(s)	918
AccB1I	GGYRCC	2 cut(s)	581, 871
AccBSI	CCGCTC	2 cut(s)	346, 1123
AccI	GTMKAC	2 cut(s)	531, 939
AccII	CGCG	6 cut(s)	25, 82, 576, 624, 944, 1072
AccIII	TCCGGA	1 cut(s)	427
AclWI	GGATC	5 cut(s)	594, 754, 888, 901, 1045
AcoI	YGGCCR	4 cut(s)	3, 474, 595, 1407
AcsI	RAATTY	1 cut(s)	731
AcvI	CACGTG	2 cut(s)	778, 825
AcyI	GRCGYC	3 cut(s)	582, 915, 933
AdeI	CACNNNGTG	1 cut(s)	825
AfaI	GTAC	3 cut(s)	452, 492, 1030
AfiI	CCNNNNNNNGG	7 cut(s)	406, 472, 837, 860, 1214, 1215, 1241
AflIII	ACRYGT	3 cut(s)	777, 801, 824
AgeI	ACCGGT	5 cut(s)	406, 1141, 1182, 1241, 1317
AgsI	TTSAA	4 cut(s)	1000, 1148, 1330, 1394
AhdI	GACNNNNNGTC	1 cut(s)	113
AjiI	CACGTC	1 cut(s)	802
AjnI	CCWGG	1 cut(s)	963
AluBI	AGCT	4 cut(s)	923, 1177, 1194, 1275
AluI	AGCT	4 cut(s)	923, 1177, 1194, 1275
Alw26I	GTCTC	2 cut(s)	632, 1394
AlwI	GGATC	5 cut(s)	594, 754, 888, 901, 1045
AlwNI	CAGNNNCTG	1 cut(s)	49
Ama87I	CYCGRG	4 cut(s)	103, 707, 1209, 1286
Aor13HI	TCCGGA	1 cut(s)	427
AoxI	GGCC	7 cut(s)	3, 474, 595, 840, 1232, 1407, 1442
ApaI	GGGCCC	1 cut(s)	1236
ApeKI	GCWGC	6 cut(s)	543, 614, 751, 856, 901, 1054
ApoI	RAATTY	1 cut(s)	731
AseI	ATTAAT	1 cut(s)	1260
AsiGI	ACCGGT	5 cut(s)	406, 1141, 1182, 1241, 1317
AspLEI	GCGC	7 cut(s)	359, 578, 584, 614, 706, 716, 1074
AsuC2I	CCSGG	5 cut(s)	104, 105, 839, 861, 1107
AsuHPI	GGTGA	5 cut(s)	48, 396, 1120, 1256, 1409
AsuNHI	GCTAGC	1 cut(s)	973
AvaI	CYCGRG	4 cut(s)	103, 707, 1209, 1286
AvaII	GGWCC	7 cut(s)	115, 373, 435, 1099, 1185, 1297, 1315
AxyI	CCTNAGG	1 cut(s)	990
BaeGI	GKGCMC	1 cut(s)	1236
BalI	TGGCCA	1 cut(s)	476
BamHI	GGATCC	1 cut(s)	893
BanI	GGYRCC	2 cut(s)	581, 871
BanII	GRGCYC	2 cut(s)	418, 1236
BauI	CACGAG	1 cut(s)	1032
BbrPI	CACGTG	2 cut(s)	778, 825
BbsI	GAAGAC	2 cut(s)	156, 1119
BbvI	GCAGC	6 cut(s)	530, 626, 738, 868, 913, 1041
BccI	CCATC	2 cut(s)	85, 485
BceAI	ACGGC	7 cut(s)	454, 473, 623, 701, 920, 1276, 1394
BcgI	CGANNNNNNTGC	2 cut(s)	1036, 1070
BciT130I	CCWGG	1 cut(s)	965
BcnI	CCSGG	5 cut(s)	104, 105, 839, 861, 1107
BcoDI	GTCTC	2 cut(s)	632, 1394
BfaI	CTAG	3 cut(s)	974, 1191, 1425
BfmI	CTRYAG	1 cut(s)	1276
BfoI	RGCGCY	2 cut(s)	585, 707
Bme1390I	CCNGG	6 cut(s)	104, 105, 839, 861, 965, 1107
Bme18I	GGWCC	7 cut(s)	115, 373, 435, 1099, 1185, 1297, 1315
BmeRI	GACNNNNNGTC	1 cut(s)	113
BmeT110I	CYCGRG	4 cut(s)	103, 707, 1209, 1286
BmgBI	CACGTC	1 cut(s)	802
BmrFI	CCNGG	6 cut(s)	104, 105, 839, 861, 965, 1107
BmrI	ACTGGG	1 cut(s)	417
BmsI	GCATC	1 cut(s)	804
BmtI	GCTAGC	1 cut(s)	977
BmuI	ACTGGG	1 cut(s)	417
BpiI	GAAGAC	2 cut(s)	156, 1119
BpuEI	CTTGAG	1 cut(s)	1153
BpuMI	CCSGG	5 cut(s)	104, 105, 839, 861, 1107
BsaAI	YACGTR	2 cut(s)	778, 825
BsaBI	GATNNNNATC	1 cut(s)	1042
BsaHI	GRCGYC	3 cut(s)	582, 915, 933
BsaI	GGTCTC	1 cut(s)	632
BsaJI	CCNNGG	4 cut(s)	23, 103, 131, 964
BsaWI	WCCGGW	9 cut(s)	406, 427, 1085, 1141, 1170, 1182, 1241, 1317, 1447
Bsc4I	CCNNNNNNNGG	7 cut(s)	406, 472, 837, 860, 1214, 1215, 1241
Bse118I	RCCGGY	9 cut(s)	406, 578, 593, 1141, 1182, 1241, 1299, 1317, 1405
Bse1I	ACTGG	2 cut(s)	423, 448
Bse21I	CCTNAGG	1 cut(s)	990
Bse8I	GATNNNNATC	1 cut(s)	1042
BseAI	TCCGGA	1 cut(s)	427
BseBI	CCWGG	1 cut(s)	965
BseDI	CCNNGG	4 cut(s)	23, 103, 131, 964
BseGI	GGATG	1 cut(s)	1362
BseJI	GATNNNNATC	1 cut(s)	1042
BseLI	CCNNNNNNNGG	7 cut(s)	406, 472, 837, 860, 1214, 1215, 1241
BseMII	CTCAG	3 cut(s)	540, 981, 1110
BseNI	ACTGG	2 cut(s)	423, 448
BseRI	GAGGAG	5 cut(s)	208, 255, 380, 389, 1285
BseSI	GKGCMC	1 cut(s)	1236
BseX3I	CGGCCG	2 cut(s)	595, 1407
BseXI	GCAGC	6 cut(s)	530, 626, 738, 868, 913, 1041
BseYI	CCCAGC	1 cut(s)	417
Bsh1236I	CGCG	6 cut(s)	25, 82, 576, 624, 944, 1072
Bsh1285I	CGRYCG	3 cut(s)	598, 1086, 1410
BshFI	GGCC	7 cut(s)	5, 476, 597, 842, 1234, 1409, 1444
BshNI	GGYRCC	2 cut(s)	581, 871
BshTI	ACCGGT	5 cut(s)	406, 1141, 1182, 1241, 1317
BsiEI	CGRYCG	3 cut(s)	598, 1086, 1410
BsiHKCI	CYCGRG	4 cut(s)	103, 707, 1209, 1286
BslFI	GGGAC	5 cut(s)	86, 101, 112, 120, 813
BslI	CCNNNNNNNGG	7 cut(s)	406, 472, 837, 860, 1214, 1215, 1241
BsmAI	GTCTC	2 cut(s)	632, 1394
BsmFI	GGGAC	5 cut(s)	86, 101, 112, 120, 813
BsmI	GAATGC	1 cut(s)	975
BsnI	GGCC	7 cut(s)	5, 476, 597, 842, 1234, 1409, 1444
Bso31I	GGTCTC	1 cut(s)	632
BsoBI	CYCGRG	4 cut(s)	103, 707, 1209, 1286
Bsp120I	GGGCCC	1 cut(s)	1232
Bsp1286I	GDGCHC	2 cut(s)	418, 1236
Bsp13I	TCCGGA	1 cut(s)	427
Bsp143I	GATC	8 cut(s)	495, 599, 675, 759, 893, 1037, 1043, 1310
Bsp19I	CCATGG	1 cut(s)	131
Bsp68I	TCGCGA	1 cut(s)	82
BspANI	GGCC	7 cut(s)	5, 476, 597, 842, 1234, 1409, 1444
BspCNI	CTCAG	3 cut(s)	539, 982, 1111
BspEI	TCCGGA	1 cut(s)	427
BspFNI	CGCG	6 cut(s)	25, 82, 576, 624, 944, 1072
BspOI	GCTAGC	1 cut(s)	977
BspPI	GGATC	5 cut(s)	594, 754, 888, 901, 1045
BspT107I	GGYRCC	2 cut(s)	581, 871
BspTNI	GGTCTC	1 cut(s)	632
BsrBI	CCGCTC	2 cut(s)	346, 1123
BsrFI	RCCGGY	9 cut(s)	406, 578, 593, 1141, 1182, 1241, 1299, 1317, 1405
BsrI	ACTGG	2 cut(s)	423, 448
BssAI	RCCGGY	9 cut(s)	406, 578, 593, 1141, 1182, 1241, 1299, 1317, 1405
BssECI	CCNNGG	4 cut(s)	23, 103, 131, 964
BssMI	GATC	8 cut(s)	495, 599, 675, 759, 893, 1037, 1043, 1310
BssNI	GRCGYC	3 cut(s)	582, 915, 933
BssSI	CACGAG	1 cut(s)	1032
BssT1I	CCWWGG	1 cut(s)	131
Bst2BI	CACGAG	1 cut(s)	1032
Bst2UI	CCWGG	1 cut(s)	965
Bst4CI	ACNGT	2 cut(s)	73, 1028
Bst6I	CTCTTC	3 cut(s)	389, 398, 512
BstACI	GRCGYC	3 cut(s)	582, 915, 933
BstBAI	YACGTR	2 cut(s)	778, 825
BstC8I	GCNNGC	4 cut(s)	467, 580, 975, 1052
BstDEI	CTNAG	3 cut(s)	526, 990, 1119
BstDSI	CCRYGG	2 cut(s)	23, 131
BstEII	GGTNACC	1 cut(s)	1137
BstF5I	GGATG	1 cut(s)	1362
BstFNI	CGCG	6 cut(s)	25, 82, 576, 624, 944, 1072
BstH2I	RGCGCY	2 cut(s)	585, 707
BstHHI	GCGC	7 cut(s)	359, 578, 584, 614, 706, 716, 1074
BstKTI	GATC	8 cut(s)	498, 602, 678, 762, 896, 1040, 1046, 1313
BstMAI	GTCTC	2 cut(s)	632, 1394
BstMBI	GATC	8 cut(s)	495, 599, 675, 759, 893, 1037, 1043, 1310
BstMCI	CGRYCG	3 cut(s)	598, 1086, 1410
BstMWI	GCNNNNNNNGC	5 cut(s)	88, 720, 814, 941, 979
BstNI	CCWGG	1 cut(s)	965
BstNSI	RCATGY	1 cut(s)	371
BstPI	GGTNACC	1 cut(s)	1137
BstSCI	CCNGG	6 cut(s)	102, 103, 837, 859, 963, 1105
BstSFI	CTRYAG	1 cut(s)	1276
BstSLI	GKGCMC	1 cut(s)	1236
BstUI	CGCG	6 cut(s)	25, 82, 576, 624, 944, 1072
BstV1I	GCAGC	6 cut(s)	530, 626, 738, 868, 913, 1041
BstV2I	GAAGAC	2 cut(s)	156, 1119
BstX2I	RGATCY	1 cut(s)	893
BstXI	CCANNNNNNTGG	1 cut(s)	370
BstYI	RGATCY	1 cut(s)	893
BstZI	CGGCCG	2 cut(s)	595, 1407
Bsu36I	CCTNAGG	1 cut(s)	990
BsuRI	GGCC	7 cut(s)	5, 476, 597, 842, 1234, 1409, 1444
BtgI	CCRYGG	2 cut(s)	23, 131
BtgZI	GCGATG	2 cut(s)	63, 469
BtrI	CACGTC	1 cut(s)	802
BtsCI	GGATG	1 cut(s)	1362
BtsIMutI	CAGTG	2 cut(s)	882, 1116
BtuMI	TCGCGA	1 cut(s)	82
Cac8I	GCNNGC	4 cut(s)	467, 580, 975, 1052
CaiI	CAGNNNCTG	1 cut(s)	49
CfoI	GCGC	7 cut(s)	359, 578, 584, 614, 706, 716, 1074
Cfr10I	RCCGGY	9 cut(s)	406, 578, 593, 1141, 1182, 1241, 1299, 1317, 1405
Cfr42I	CCGCGG	1 cut(s)	26
Cfr9I	CCCGGG	1 cut(s)	103
CpoI	CGGWCCG	1 cut(s)	1315
CseI	GACGC	4 cut(s)	559, 941, 950, 1021
Csp6I	GTAC	3 cut(s)	451, 491, 1029
CspAI	ACCGGT	5 cut(s)	406, 1141, 1182, 1241, 1317
CspI	CGGWCCG	1 cut(s)	1315
CviAII	CATG	5 cut(s)	38, 132, 337, 368, 514
CviQI	GTAC	3 cut(s)	451, 491, 1029
DdeI	CTNAG	3 cut(s)	526, 990, 1119
DinI	GGCGCC	1 cut(s)	583
DpnI	GATC	8 cut(s)	497, 601, 677, 761, 895, 1039, 1045, 1312
DpnII	GATC	8 cut(s)	495, 599, 675, 759, 893, 1037, 1043, 1310
DraIII	CACNNNGTG	1 cut(s)	825
DrdI	GACNNNNNNGTC	1 cut(s)	638
DriI	GACNNNNNGTC	1 cut(s)	113
DseDI	GACNNNNNNGTC	1 cut(s)	638
EaeI	YGGCCR	4 cut(s)	3, 474, 595, 1407
EagI	CGGCCG	2 cut(s)	595, 1407
Eam1104I	CTCTTC	3 cut(s)	389, 398, 512
Eam1105I	GACNNNNNGTC	1 cut(s)	113
EarI	CTCTTC	3 cut(s)	389, 398, 512
EciI	GGCGGA	1 cut(s)	527
EclXI	CGGCCG	2 cut(s)	595, 1407
Eco130I	CCWWGG	1 cut(s)	131
Eco24I	GRGCYC	2 cut(s)	418, 1236
Eco31I	GGTCTC	1 cut(s)	632
Eco47I	GGWCC	7 cut(s)	115, 373, 435, 1099, 1185, 1297, 1315
Eco52I	CGGCCG	2 cut(s)	595, 1407
Eco72I	CACGTG	2 cut(s)	778, 825
Eco81I	CCTNAGG	1 cut(s)	990
Eco88I	CYCGRG	4 cut(s)	103, 707, 1209, 1286
Eco91I	GGTNACC	1 cut(s)	1137
EcoO109I	RGGNCCY	2 cut(s)	115, 1233
EcoO65I	GGTNACC	1 cut(s)	1137
EcoRI	GAATTC	1 cut(s)	731
EcoRII	CCWGG	1 cut(s)	963
EcoT14I	CCWWGG	1 cut(s)	131
EcoT38I	GRGCYC	2 cut(s)	418, 1236
EgeI	GGCGCC	1 cut(s)	583
EheI	GGCGCC	1 cut(s)	583
ErhI	CCWWGG	1 cut(s)	131
FaeI	CATG	5 cut(s)	41, 135, 340, 371, 517
FaiI	YATR	9 cut(s)	39, 133, 258, 296, 338, 369, 515, 1004, 1166
FaqI	GGGAC	5 cut(s)	86, 101, 112, 120, 813
FatI	CATG	5 cut(s)	37, 131, 336, 367, 513
FauI	CCCGC	2 cut(s)	105, 474
FblI	GTMKAC	2 cut(s)	531, 939
FokI	GGATG	1 cut(s)	1369
FriOI	GRGCYC	2 cut(s)	418, 1236
FspBI	CTAG	3 cut(s)	974, 1191, 1425
GlaI	GCGC	7 cut(s)	358, 577, 583, 613, 705, 715, 1073
GsaI	CCCAGC	1 cut(s)	421
HaeII	RGCGCY	2 cut(s)	585, 707
HaeIII	GGCC	7 cut(s)	5, 476, 597, 842, 1234, 1409, 1444
HgaI	GACGC	4 cut(s)	559, 941, 950, 1021
HhaI	GCGC	7 cut(s)	359, 578, 584, 614, 706, 716, 1074
Hin1I	GRCGYC	3 cut(s)	582, 915, 933
Hin1II	CATG	5 cut(s)	41, 135, 340, 371, 517
Hin6I	GCGC	7 cut(s)	357, 576, 582, 612, 704, 714, 1072
HinP1I	GCGC	7 cut(s)	357, 576, 582, 612, 704, 714, 1072
HinfI	GANTC	1 cut(s)	866
HphI	GGTGA	5 cut(s)	48, 396, 1120, 1256, 1409
Hpy166II	GTNNAC	4 cut(s)	532, 940, 1128, 1188
Hpy188I	TCNGA	5 cut(s)	439, 550, 898, 1023, 1315
Hpy188III	TCNNGA	6 cut(s)	81, 428, 603, 629, 1041, 1153
Hpy8I	GTNNAC	4 cut(s)	532, 940, 1128, 1188
Hpy99I	CGWCG	8 cut(s)	210, 609, 746, 803, 917, 920, 935, 1375
HpyAV	CCTTC	9 cut(s)	165, 191, 209, 283, 300, 661, 1208, 1275, 1388
HpyCH4III	ACNGT	2 cut(s)	73, 1028
HpyCH4IV	ACGT	6 cut(s)	208, 534, 777, 801, 824, 915
HpyCH4V	TGCA	1 cut(s)	795
HpyF10VI	GCNNNNNNNGC	5 cut(s)	88, 720, 814, 941, 979
HpyF3I	CTNAG	3 cut(s)	526, 990, 1119
HpySE526I	ACGT	6 cut(s)	208, 534, 777, 801, 824, 915
Hsp92I	GRCGYC	3 cut(s)	582, 915, 933
Hsp92II	CATG	5 cut(s)	41, 135, 340, 371, 517
HspAI	GCGC	7 cut(s)	357, 576, 582, 612, 704, 714, 1072
KasI	GGCGCC	1 cut(s)	581
KflI	GGGWCCC	1 cut(s)	115
Kpn2I	TCCGGA	1 cut(s)	427
KroI	GCCGGC	1 cut(s)	578
KroNI	GCCGGC	1 cut(s)	580
KspI	CCGCGG	1 cut(s)	26
Kzo9I	GATC	8 cut(s)	495, 599, 675, 759, 893, 1037, 1043, 1310
LmnI	GCTCC	5 cut(s)	346, 413, 551, 1272, 1428
Lsp1109I	GCAGC	6 cut(s)	530, 626, 738, 868, 913, 1041
LweI	GCATC	1 cut(s)	804
MaeI	CTAG	3 cut(s)	974, 1191, 1425
MaeII	ACGT	6 cut(s)	208, 534, 777, 801, 824, 915
MaeIII	GTNAC	2 cut(s)	209, 1137
MalI	GATC	8 cut(s)	497, 601, 677, 761, 895, 1039, 1045, 1312
MbiI	CCGCTC	2 cut(s)	346, 1123
MboI	GATC	8 cut(s)	495, 599, 675, 759, 893, 1037, 1043, 1310
MboII	GAAGA	7 cut(s)	150, 156, 376, 385, 499, 514, 1124
MfeI	CAATTG	1 cut(s)	1389
MflI	RGATCY	1 cut(s)	893
MhlI	GDGCHC	2 cut(s)	418, 1236
MlsI	TGGCCA	1 cut(s)	476
MluCI	AATT	3 cut(s)	731, 1304, 1389
MluNI	TGGCCA	1 cut(s)	476
Mly113I	GGCGCC	1 cut(s)	582
MlyI	GAGTC	1 cut(s)	860
MmeI	TCCRAC	5 cut(s)	391, 462, 573, 1126, 1275
Mox20I	TGGCCA	1 cut(s)	476
MreI	CGCCGGCG	1 cut(s)	578
MroI	TCCGGA	1 cut(s)	427
MroNI	GCCGGC	1 cut(s)	578
MscI	TGGCCA	1 cut(s)	476
MseI	TTAA	4 cut(s)	687, 725, 1260, 1453
MslI	CAYNNNNRTG	2 cut(s)	800, 1355
Msp20I	TGGCCA	1 cut(s)	476
MspA1I	CMGCKG	4 cut(s)	25, 49, 457, 543
MspR9I	CCNGG	6 cut(s)	104, 105, 839, 861, 965, 1107
MunI	CAATTG	1 cut(s)	1389
Mva1269I	GAATGC	1 cut(s)	975
MvaI	CCWGG	1 cut(s)	965
MvnI	CGCG	6 cut(s)	25, 82, 576, 624, 944, 1072
MwoI	GCNNNNNNNGC	5 cut(s)	88, 720, 814, 941, 979
NaeI	GCCGGC	1 cut(s)	580
NarI	GGCGCC	1 cut(s)	582
NciI	CCSGG	5 cut(s)	104, 105, 839, 861, 1107
NcoI	CCATGG	1 cut(s)	131
NdeII	GATC	8 cut(s)	495, 599, 675, 759, 893, 1037, 1043, 1310
NgoMIV	GCCGGC	1 cut(s)	578
NheI	GCTAGC	1 cut(s)	973
NlaIII	CATG	5 cut(s)	41, 135, 340, 371, 517
NmeAIII	GCCGAG	4 cut(s)	31, 697, 848, 1420
NruI	TCGCGA	1 cut(s)	82
NspI	RCATGY	1 cut(s)	371
PaeR7I	CTCGAG	2 cut(s)	707, 1286
PcsI	WCGNNNNNNNCGW	4 cut(s)	636, 714, 741, 821
PctI	GAATGC	1 cut(s)	975
PdiI	GCCGGC	1 cut(s)	580
PflFI	GACNNNGTC	1 cut(s)	569
PinAI	ACCGGT	5 cut(s)	406, 1141, 1182, 1241, 1317
PleI	GAGTC	1 cut(s)	860
PluTI	GGCGCC	1 cut(s)	585
PmaCI	CACGTG	2 cut(s)	778, 825
PmlI	CACGTG	2 cut(s)	778, 825
PpsI	GAGTC	1 cut(s)	860
Ppu21I	YACGTR	2 cut(s)	778, 825
PpuMI	RGGWCCY	1 cut(s)	115
PshBI	ATTAAT	1 cut(s)	1260
PsiI	TTATAA	1 cut(s)	258
Psp5II	RGGWCCY	1 cut(s)	115
Psp6I	CCWGG	1 cut(s)	963
PspCI	CACGTG	2 cut(s)	778, 825
PspEI	GGTNACC	1 cut(s)	1137
PspFI	CCCAGC	1 cut(s)	417
PspGI	CCWGG	1 cut(s)	963
PspOMI	GGGCCC	1 cut(s)	1232
PspPPI	RGGWCCY	1 cut(s)	115
PspXI	VCTCGAGB	2 cut(s)	707, 1286
PsrI	GAACNNNNNNTAC	2 cut(s)	443, 475
PstNI	CAGNNNCTG	1 cut(s)	49
PsuI	RGATCY	1 cut(s)	893
PsyI	GACNNNGTC	1 cut(s)	569
RruI	TCGCGA	1 cut(s)	82
RsaI	GTAC	3 cut(s)	452, 492, 1030
RsaNI	GTAC	3 cut(s)	451, 491, 1029
RseI	CAYNNNNRTG	2 cut(s)	800, 1355
Rsr2I	CGGWCCG	1 cut(s)	1315
RsrII	CGGWCCG	1 cut(s)	1315
SacII	CCGCGG	1 cut(s)	26
SaqAI	TTAA	4 cut(s)	687, 725, 1260, 1453
Sau3AI	GATC	8 cut(s)	495, 599, 675, 759, 893, 1037, 1043, 1310
SchI	GAGTC	1 cut(s)	860
ScrFI	CCNGG	6 cut(s)	104, 105, 839, 861, 965, 1107
SduI	GDGCHC	2 cut(s)	418, 1236
SfaNI	GCATC	1 cut(s)	804
SfcI	CTRYAG	1 cut(s)	1276
SfoI	GGCGCC	1 cut(s)	583
Sfr274I	CTCGAG	2 cut(s)	707, 1286
Sfr303I	CCGCGG	1 cut(s)	26
SgrAI	CRCCGGYG	1 cut(s)	578
SgrBI	CCGCGG	1 cut(s)	26
SinI	GGWCC	7 cut(s)	115, 373, 435, 1099, 1185, 1297, 1315
SlaI	CTCGAG	2 cut(s)	707, 1286
SmaI	CCCGGG	1 cut(s)	105
SmiMI	CAYNNNNRTG	2 cut(s)	800, 1355
SmlI	CTYRAG	3 cut(s)	707, 1132, 1286
SmoI	CTYRAG	3 cut(s)	707, 1132, 1286
Sse9I	AATT	3 cut(s)	731, 1304, 1389
SspDI	GGCGCC	1 cut(s)	581
SspMI	CTAG	3 cut(s)	974, 1191, 1425
StyD4I	CCNGG	6 cut(s)	102, 103, 837, 859, 963, 1105
StyI	CCWWGG	1 cut(s)	131
TaaI	ACNGT	2 cut(s)	73, 1028
TaiI	ACGT	6 cut(s)	211, 537, 780, 804, 827, 918
TasI	AATT	3 cut(s)	731, 1304, 1389
TauI	GCSGC	6 cut(s)	25, 49, 362, 503, 543, 1388
Tru1I	TTAA	4 cut(s)	687, 725, 1260, 1453
Tru9I	TTAA	4 cut(s)	687, 725, 1260, 1453
TscAI	CASTG	2 cut(s)	889, 1123
TseI	GCWGC	6 cut(s)	543, 614, 751, 856, 901, 1054
TspDTI	ATGAA	2 cut(s)	241, 1181
TspMI	CCCGGG	1 cut(s)	103
TspRI	CASTG	2 cut(s)	889, 1123
Tth111I	GACNNNGTC	1 cut(s)	569
VpaK11BI	GGWCC	7 cut(s)	115, 373, 435, 1099, 1185, 1297, 1315
VspI	ATTAAT	1 cut(s)	1260
XapI	RAATTY	1 cut(s)	731
XceI	RCATGY	1 cut(s)	371
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1195
XhoI	CTCGAG	2 cut(s)	707, 1286
XmaI	CCCGGG	1 cut(s)	103
XmiI	GTMKAC	2 cut(s)	531, 939
XspI	CTAG	3 cut(s)	974, 1191, 1425
ZraI	GACGTC	1 cut(s)	916

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.