MD13G1004800.v1.1

Metal dependent phosphohydrolases with conserved 'HD' motif.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0013001
Physical Location & Seq
Reverse (-)
298494 .. 301852
3359 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1004800.v1.1.491

Sequence Viewer

Length: 732 bp
ATGGGGTCATTGGGATGGGAAGAGACGGTGAGAAAGGCGGAGAAGCTGGTGGAGGAAGCCATGAAGGAGAACGACGCGTCCCACGACGCTGCTCACGTGTGGCGGGTCCGAGACCTCGCTCTCTCTCTCGCCCGTGAAGAAGGCCTCTCTTCCAATTCCGACTCCATGCAAATTATAGAAGTGGCTGCACTTCTCCATGATATAGGTGATTACAAGTACTTGAGAGATCCCTCAGAGGAAAAAATTGTTGAGAACTTTCTCGATGAGGAGGGCATAGATGAGACTAAGAAAATGAAGATTATAACGATCATCAAGGGAATGGGTTTCAAAGATGAGCTTGCAGGAACAAATAACGATTTTCCTCCAGAATTTGGGGTTGTTCAAGATGCTGATCGTCTCGATGCAATTGGTGCTATTGGAATTGCTCGTTGCTTTACTTTTGGTGGAAGTAGGAACAGAGTGCTACATGATCCTAAGATTCTGCCACGATGTGACTTATCCAAGGAAAAGTACATGAAAAAGGATGAGCAGACTACTGTGAATCACTTTCACGAGAAGCTTCTTAAGCTAAAGGCTTTGATGAAAACAAAGGCTGGGCAGAAGAGAGCTGAGAGACGGCACAAGTTCATGGAGGAGTTTCTTGTAGAGTTCTATGAAGAAACTTGTCAACTTCGCTCCTGGAGCGGAACTTGCTTGAAGATGCGGGCTATAATATTCAAACCAACTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HD

Protein Analysis

244

Amino Acids

27.82

Weight (kDa)

6.04

Isoelectric Point (pI)

36.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HD PF01966 31 - 135 2.4e-09 HD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013001)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17330
fragaria_vesca FvH4_4g36970 FvH4_4g36970 FvH4_4g36970
malus_domestica MD13G1004800.v1.1
prunus_persica Prupe.1G347900_v2.0.a1
pyrus_communis pycom13g00420
rosa_chinensis RchiOBHm_Chr4g0446761
rosa_laevigata RLG00000005634
rosa_multiflora Rmu_sc0001753.1_g000032
rosa_roxburghii Rroxscaffold_5G00387080
rosa_rugosa Rorug04G0374400 Rorug04G0374400
rosa_samantha Rh4AG435800 Rh4BG444700 Rh4CG461900 Rh4DG443600
rosa_wichuraiana Rw4G037250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 302
AccB7I CCANNNNNTGG 1 cut(s) 371
AccBSI CCGCTC 1 cut(s) 684
AccII CGCG 1 cut(s) 77
AciI CCGC 4 cut(s) 38, 103, 684, 703
AclWI GGATC 2 cut(s) 221, 464
AcsI RAATTY 1 cut(s) 368
AcvI CACGTG 1 cut(s) 97
AdeI CACNNNGTG 1 cut(s) 491
AfaI GTAC 2 cut(s) 218, 512
AfiI CCNNNNNNNGG 1 cut(s) 371
AflII CTTAAG 1 cut(s) 563
AflIII ACRYGT 2 cut(s) 75, 96
AgsI TTSAA 4 cut(s) 328, 383, 697, 718
AjnI CCWGG 1 cut(s) 677
AloI GAACNNNNNNTCC 2 cut(s) 62, 94
AluBI AGCT 5 cut(s) 46, 337, 559, 568, 608
AluI AGCT 5 cut(s) 46, 337, 559, 568, 608
Alw26I GTCTC 5 cut(s) 17, 105, 275, 401, 607
AlwI GGATC 2 cut(s) 221, 464
AoxI GGCC 1 cut(s) 142
ApeKI GCWGC 2 cut(s) 89, 185
ApoI RAATTY 1 cut(s) 368
AspS9I GGNCC 1 cut(s) 106
AsuHPI GGTGA 2 cut(s) 40, 218
AvaII GGWCC 1 cut(s) 106
BauI CACGAG 1 cut(s) 551
BbrPI CACGTG 1 cut(s) 97
BbvI GCAGC 2 cut(s) 76, 172
BccI CCATC 1 cut(s) 9
BceAI ACGGC 1 cut(s) 632
BciT130I CCWGG 1 cut(s) 679
BcoDI GTCTC 5 cut(s) 17, 105, 275, 401, 607
BfrI CTTAAG 1 cut(s) 563
BisI GCNGC 2 cut(s) 90, 186
BlsI GCNGC 2 cut(s) 91, 187
BmcAI AGTACT 1 cut(s) 218
Bme1390I CCNGG 1 cut(s) 679
Bme18I GGWCC 1 cut(s) 106
BmgT120I GGNCC 1 cut(s) 106
BmiI GGNNCC 1 cut(s) 107
BmrFI CCNGG 1 cut(s) 679
BmsI GCATC 3 cut(s) 376, 391, 690
BpmI CTGGAG 2 cut(s) 348, 700
BpuEI CTTGAG 1 cut(s) 241
BsaAI YACGTR 1 cut(s) 97
BsaBI GATNNNNATC 1 cut(s) 390
BsaI GGTCTC 1 cut(s) 105
BsaJI CCNNGG 1 cut(s) 501
Bsc4I CCNNNNNNNGG 1 cut(s) 371
Bse8I GATNNNNATC 1 cut(s) 390
BseBI CCWGG 1 cut(s) 679
BseDI CCNNGG 1 cut(s) 501
BseGI GGATG 2 cut(s) 20, 529
BseJI GATNNNNATC 1 cut(s) 390
BseLI CCNNNNNNNGG 1 cut(s) 371
BseMII CTCAG 2 cut(s) 246, 600
BseRI GAGGAG 2 cut(s) 281, 647
BseXI GCAGC 2 cut(s) 76, 172
BseYI CCCAGC 1 cut(s) 593
BsgI GTGCAG 1 cut(s) 171
Bsh1236I CGCG 1 cut(s) 77
BshFI GGCC 1 cut(s) 144
BslFI GGGAC 1 cut(s) 64
BslI CCNNNNNNNGG 1 cut(s) 371
BsmAI GTCTC 5 cut(s) 17, 105, 275, 401, 607
BsmBI CGTCTC 3 cut(s) 17, 401, 607
BsmFI GGGAC 1 cut(s) 64
BsnI GGCC 1 cut(s) 144
Bso31I GGTCTC 1 cut(s) 105
Bsp143I GATC 4 cut(s) 226, 306, 391, 469
BspACI CCGC 4 cut(s) 38, 103, 684, 703
BspANI GGCC 1 cut(s) 144
BspCNI CTCAG 2 cut(s) 245, 601
BspFNI CGCG 1 cut(s) 77
BspLI GGNNCC 1 cut(s) 107
BspPI GGATC 2 cut(s) 221, 464
BspTI CTTAAG 1 cut(s) 563
BspTNI GGTCTC 1 cut(s) 105
BsrBI CCGCTC 1 cut(s) 684
BssECI CCNNGG 1 cut(s) 501
BssMI GATC 4 cut(s) 226, 306, 391, 469
BssSI CACGAG 1 cut(s) 551
BssT1I CCWWGG 1 cut(s) 501
Bst2BI CACGAG 1 cut(s) 551
Bst2UI CCWGG 1 cut(s) 679
Bst4CI ACNGT 3 cut(s) 28, 538, 727
Bst6I CTCTTC 3 cut(s) 15, 154, 596
BstAFI CTTAAG 1 cut(s) 563
BstAPI GCANNNNNTGC 1 cut(s) 410
BstBAI YACGTR 1 cut(s) 97
BstC8I GCNNGC 2 cut(s) 339, 705
BstDEI CTNAG 4 cut(s) 232, 285, 474, 609
BstF5I GGATG 2 cut(s) 20, 529
BstFNI CGCG 1 cut(s) 77
BstKTI GATC 4 cut(s) 229, 309, 394, 472
BstMAI GTCTC 5 cut(s) 17, 105, 275, 401, 607
BstMBI GATC 4 cut(s) 226, 306, 391, 469
BstMWI GCNNNNNNNGC 4 cut(s) 410, 565, 681, 690
BstNI CCWGG 1 cut(s) 679
BstSCI CCNGG 1 cut(s) 677
BstUI CGCG 1 cut(s) 77
BstV1I GCAGC 2 cut(s) 76, 172
BstX2I RGATCY 1 cut(s) 226
BstYI RGATCY 1 cut(s) 226
BsuRI GGCC 1 cut(s) 144
BtsCI GGATG 2 cut(s) 20, 529
Cac8I GCNNGC 2 cut(s) 339, 705
Cfr13I GGNCC 1 cut(s) 106
CseI GACGC 3 cut(s) 66, 83, 95
Csp6I GTAC 2 cut(s) 217, 511
CviAII CATG 6 cut(s) 61, 166, 197, 467, 514, 628
CviQI GTAC 2 cut(s) 217, 511
DdeI CTNAG 4 cut(s) 232, 285, 474, 609
DpnI GATC 4 cut(s) 228, 308, 393, 471
DpnII GATC 4 cut(s) 226, 306, 391, 469
DraIII CACNNNGTG 1 cut(s) 491
Eam1104I CTCTTC 3 cut(s) 15, 154, 596
EarI CTCTTC 3 cut(s) 15, 154, 596
EciI GGCGGA 1 cut(s) 53
Eco130I CCWWGG 1 cut(s) 501
Eco147I AGGCCT 1 cut(s) 144
Eco31I GGTCTC 1 cut(s) 105
Eco47I GGWCC 1 cut(s) 106
Eco72I CACGTG 1 cut(s) 97
EcoRII CCWGG 1 cut(s) 677
EcoT14I CCWWGG 1 cut(s) 501
ErhI CCWWGG 1 cut(s) 501
Esp3I CGTCTC 3 cut(s) 17, 401, 607
FaeI CATG 6 cut(s) 64, 169, 200, 470, 517, 631
FalI AAGNNNNNCTT 2 cut(s) 321, 353
FaqI GGGAC 1 cut(s) 64
FatI CATG 6 cut(s) 60, 165, 196, 466, 513, 627
FauI CCCGC 2 cut(s) 96, 696
Fnu4HI GCNGC 2 cut(s) 90, 186
FokI GGATG 2 cut(s) 27, 536
Fsp4HI GCNGC 2 cut(s) 90, 186
GluI GCNGC 2 cut(s) 90, 186
GsaI CCCAGC 1 cut(s) 597
GsuI CTGGAG 2 cut(s) 348, 700
HaeIII GGCC 1 cut(s) 144
HgaI GACGC 3 cut(s) 66, 83, 95
Hin1II CATG 6 cut(s) 64, 169, 200, 470, 517, 631
HincII GTYRAC 1 cut(s) 668
HindII GTYRAC 1 cut(s) 668
HindIII AAGCTT 1 cut(s) 557
HinfI GANTC 3 cut(s) 161, 478, 541
HphI GGTGA 2 cut(s) 40, 218
Hpy166II GTNNAC 1 cut(s) 668
Hpy188I TCNGA 3 cut(s) 110, 160, 235
Hpy188III TCNNGA 5 cut(s) 260, 365, 383, 398, 551
Hpy8I GTNNAC 1 cut(s) 668
Hpy99I CGWCG 2 cut(s) 77, 89
HpyAV CCTTC 2 cut(s) 58, 134
HpyCH4III ACNGT 3 cut(s) 28, 538, 727
HpyCH4IV ACGT 1 cut(s) 96
HpyCH4V TGCA 4 cut(s) 169, 188, 341, 404
HpyF10VI GCNNNNNNNGC 4 cut(s) 410, 565, 681, 690
HpyF3I CTNAG 4 cut(s) 232, 285, 474, 609
HpySE526I ACGT 1 cut(s) 96
Hsp92II CATG 6 cut(s) 64, 169, 200, 470, 517, 631
Kzo9I GATC 4 cut(s) 226, 306, 391, 469
LmnI GCTCC 2 cut(s) 680, 681
LpnPI CCDG 6 cut(s) 32, 327, 378, 579, 664, 691
Lsp1109I GCAGC 2 cut(s) 76, 172
LweI GCATC 3 cut(s) 376, 391, 690
MaeII ACGT 1 cut(s) 96
MaeIII GTNAC 1 cut(s) 491
MalI GATC 4 cut(s) 228, 308, 393, 471
MbiI CCGCTC 1 cut(s) 684
MboI GATC 4 cut(s) 226, 306, 391, 469
MboII GAAGA 7 cut(s) 32, 141, 149, 307, 613, 668, 709
MfeI CAATTG 1 cut(s) 405
MflI RGATCY 1 cut(s) 226
MluCI AATT 6 cut(s) 154, 171, 243, 368, 405, 420
MluI ACGCGT 1 cut(s) 75
MlyI GAGTC 1 cut(s) 155
MmeI TCCRAC 1 cut(s) 183
MnlI CCTC 9 cut(s) 46, 125, 155, 229, 241, 259, 262, 372, 625
MseI TTAA 1 cut(s) 564
MslI CAYNNNNRTG 1 cut(s) 13
MspCI CTTAAG 1 cut(s) 563
MspR9I CCNGG 1 cut(s) 679
MunI CAATTG 1 cut(s) 405
MvaI CCWGG 1 cut(s) 679
MvnI CGCG 1 cut(s) 77
MwoI GCNNNNNNNGC 4 cut(s) 410, 565, 681, 690
NdeII GATC 4 cut(s) 226, 306, 391, 469
NlaIII CATG 6 cut(s) 64, 169, 200, 470, 517, 631
NlaIV GGNNCC 1 cut(s) 107
NmuCI GTSAC 1 cut(s) 491
PceI AGGCCT 1 cut(s) 144
PcsI WCGNNNNNNNCGW 2 cut(s) 81, 93
PfeI GAWTC 2 cut(s) 478, 541
PflMI CCANNNNNTGG 1 cut(s) 371
PfoI TCCNGGA 1 cut(s) 677
PkrI GCNGC 2 cut(s) 91, 187
PleI GAGTC 1 cut(s) 155
PmaCI CACGTG 1 cut(s) 97
PmlI CACGTG 1 cut(s) 97
PpsI GAGTC 1 cut(s) 155
Ppu21I YACGTR 1 cut(s) 97
PsiI TTATAA 1 cut(s) 302
Psp6I CCWGG 1 cut(s) 677
PspCI CACGTG 1 cut(s) 97
PspFI CCCAGC 1 cut(s) 593
PspGI CCWGG 1 cut(s) 677
PspN4I GGNNCC 1 cut(s) 107
PspPI GGNCC 1 cut(s) 106
PsuI RGATCY 1 cut(s) 226
RsaI GTAC 2 cut(s) 218, 512
RsaNI GTAC 2 cut(s) 217, 511
RseI CAYNNNNRTG 1 cut(s) 13
SaqAI TTAA 1 cut(s) 564
SatI GCNGC 2 cut(s) 90, 186
Sau3AI GATC 4 cut(s) 226, 306, 391, 469
Sau96I GGNCC 1 cut(s) 106
ScaI AGTACT 1 cut(s) 218
SchI GAGTC 1 cut(s) 155
ScrFI CCNGG 1 cut(s) 679
SetI ASST 8 cut(s) 48, 99, 117, 208, 339, 561, 570, 610
SfaNI GCATC 3 cut(s) 376, 391, 690
SinI GGWCC 1 cut(s) 106
SmiMI CAYNNNNRTG 1 cut(s) 13
SmlI CTYRAG 2 cut(s) 220, 563
SmoI CTYRAG 2 cut(s) 220, 563
Sse9I AATT 6 cut(s) 154, 171, 243, 368, 405, 420
SseBI AGGCCT 1 cut(s) 144
SsiI CCGC 4 cut(s) 38, 103, 684, 703
SspI AATATT 1 cut(s) 714
StuI AGGCCT 1 cut(s) 144
StyD4I CCNGG 1 cut(s) 677
StyI CCWWGG 1 cut(s) 501
TaaI ACNGT 3 cut(s) 28, 538, 727
TaiI ACGT 1 cut(s) 99
TaqI TCGA 2 cut(s) 261, 399
TasI AATT 6 cut(s) 154, 171, 243, 368, 405, 420
TatI WGTACW 2 cut(s) 216, 510
TfiI GAWTC 2 cut(s) 478, 541
Tru1I TTAA 1 cut(s) 564
Tru9I TTAA 1 cut(s) 564
TseFI GTSAC 1 cut(s) 491
TseI GCWGC 2 cut(s) 89, 185
Tsp45I GTSAC 1 cut(s) 491
TspDTI ATGAA 6 cut(s) 77, 308, 530, 596, 616, 669
Van91I CCANNNNNTGG 1 cut(s) 371
Vha464I CTTAAG 1 cut(s) 563
VpaK11BI GGWCC 1 cut(s) 106
XapI RAATTY 1 cut(s) 368
ZrmI AGTACT 1 cut(s) 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.