Rroxscaffold_5G00387080

Metal dependent phosphohydrolases with conserved 'HD' motif.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0013001
Physical Location & Seq
Forward (+)
65827980 .. 65829963
1984 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00387080.1

Sequence Viewer

Length: 702 bp
ATGGCGAGCTGGGATTGGGAAGAGACGGTGAGAAAGGCGGAGGAGTTGGTCGTAAAATCCATGAAAGGCAATGACGCATCACACGACCCAGCCCACGTATGGAGGGTGAGAGACCTCGCACTCTCTCTTGCTCGCGAAGAACAAGCTCTCTCCTCCAACTCCGACTCCATGCAAATTTCTTTTCTTACTCGCCAGGTAGAACTTGCTGCACTCCTCCATGATATAGGTGATTATAAGTACATGAGAGATCCTTCGGAGGACAAACTTGTTGAGCATTTTCTTATTGACTCAGGCATAGAGGAGAGCAAGAAAACAAAGATTATAAAGATCATCAATGGAATGGGGTTTAAAGATGAGCTGGCTGGGCTGGCAAATAGTGATCTTCCTCTAGAATTTGGGGTTGTACAAGATGCTGATCGGCTTGATGCAATTGGTGCTATAGGAATTGCTCGTTGCTTTACTTTTGGAGGAAGTAGAAATAGAGTGCTACATGATCCTAACATTCAGCCACGATCTGATTTATCCAAGGAAAAGTACATGAAAAAAGAGGAGCAAACTACTGTCAATCACTTTCATGAGAAGCTTCTCAAGCTAAAGGATCTGATGAAAACAAAGGCTGGACGGAAGAGAGCTGAGAAAAGGCACAAGTTCATGGAGGAGTTTCTTGTAGAGTTCTATGAAGAGTGGGATGGGAGAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HD

Protein Analysis

233

Amino Acids

26.78

Weight (kDa)

5.95

Isoelectric Point (pI)

38.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HD PF01966 32 - 143 2.5e-07 HD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013001)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17330
fragaria_vesca FvH4_4g36970 FvH4_4g36970 FvH4_4g36970
malus_domestica MD13G1004800.v1.1
prunus_persica Prupe.1G347900_v2.0.a1
pyrus_communis pycom13g00420
rosa_chinensis RchiOBHm_Chr4g0446761
rosa_laevigata RLG00000005634
rosa_multiflora Rmu_sc0001753.1_g000032
rosa_roxburghii Rroxscaffold_5G00387080
rosa_rugosa Rorug04G0374400 Rorug04G0374400
rosa_samantha Rh4AG435800 Rh4BG444700 Rh4CG461900 Rh4DG443600
rosa_wichuraiana Rw4G037250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 234, 323
AccII CGCG 1 cut(s) 135
AciI CCGC 1 cut(s) 38
AclWI GGATC 3 cut(s) 242, 488, 606
AcsI RAATTY 2 cut(s) 174, 392
AfaI GTAC 3 cut(s) 239, 405, 536
AfiI CCNNNNNNNGG 1 cut(s) 99
AjnI CCWGG 1 cut(s) 192
AluBI AGCT 7 cut(s) 9, 146, 358, 583, 592, 632, 698
AluI AGCT 7 cut(s) 9, 146, 358, 583, 592, 632, 698
Alw26I GTCTC 2 cut(s) 17, 105
AlwI GGATC 3 cut(s) 242, 488, 606
ApeKI GCWGC 1 cut(s) 206
ApoI RAATTY 2 cut(s) 174, 392
AsuHPI GGTGA 3 cut(s) 40, 118, 239
BbvI GCAGC 1 cut(s) 193
BccI CCATC 1 cut(s) 683
BciT130I CCWGG 1 cut(s) 194
BcoDI GTCTC 2 cut(s) 17, 105
BfaI CTAG 1 cut(s) 389
BfmI CTRYAG 1 cut(s) 438
BisI GCNGC 1 cut(s) 207
BlsI GCNGC 1 cut(s) 208
Bme1390I CCNGG 1 cut(s) 194
BmrFI CCNGG 1 cut(s) 194
BmsI GCATC 3 cut(s) 86, 400, 415
BpuEI CTTGAG 1 cut(s) 572
BsaAI YACGTR 1 cut(s) 97
BsaBI GATNNNNATC 1 cut(s) 414
BsaI GGTCTC 1 cut(s) 105
BsaJI CCNNGG 1 cut(s) 525
BsaXI ACNNNNNCTCC 2 cut(s) 149, 179
Bsc4I CCNNNNNNNGG 1 cut(s) 99
Bse3DI GCAATG 1 cut(s) 76
Bse8I GATNNNNATC 1 cut(s) 414
BseBI CCWGG 1 cut(s) 194
BseDI CCNNGG 1 cut(s) 525
BseGI GGATG 1 cut(s) 694
BseJI GATNNNNATC 1 cut(s) 414
BseLI CCNNNNNNNGG 1 cut(s) 99
BseMI GCAATG 1 cut(s) 76
BseMII CTCAG 2 cut(s) 303, 624
BseRI GAGGAG 6 cut(s) 56, 142, 203, 314, 563, 671
BseXI GCAGC 1 cut(s) 193
BseYI CCCAGC 3 cut(s) 9, 88, 362
BsgI GTGCAG 1 cut(s) 192
Bsh1236I CGCG 1 cut(s) 135
BslI CCNNNNNNNGG 1 cut(s) 99
BsmAI GTCTC 2 cut(s) 17, 105
BsmBI CGTCTC 1 cut(s) 17
Bso31I GGTCTC 1 cut(s) 105
Bsp1407I TGTACA 1 cut(s) 403
Bsp143I GATC 7 cut(s) 247, 327, 379, 415, 493, 512, 598
Bsp68I TCGCGA 1 cut(s) 135
BspACI CCGC 1 cut(s) 38
BspCNI CTCAG 2 cut(s) 302, 625
BspFNI CGCG 1 cut(s) 135
BspHI TCATGA 1 cut(s) 574
BspPI GGATC 3 cut(s) 242, 488, 606
BspTNI GGTCTC 1 cut(s) 105
BsrDI GCAATG 1 cut(s) 76
BsrGI TGTACA 1 cut(s) 403
BssECI CCNNGG 1 cut(s) 525
BssMI GATC 7 cut(s) 247, 327, 379, 415, 493, 512, 598
BssT1I CCWWGG 1 cut(s) 525
Bst2UI CCWGG 1 cut(s) 194
Bst4CI ACNGT 2 cut(s) 28, 562
Bst6I CTCTTC 3 cut(s) 15, 620, 675
BstAPI GCANNNNNTGC 1 cut(s) 434
BstAUI TGTACA 1 cut(s) 403
BstBAI YACGTR 1 cut(s) 97
BstC8I GCNNGC 4 cut(s) 7, 133, 360, 369
BstDEI CTNAG 2 cut(s) 289, 633
BstF5I GGATG 1 cut(s) 694
BstFNI CGCG 1 cut(s) 135
BstKTI GATC 7 cut(s) 250, 330, 382, 418, 496, 515, 601
BstMAI GTCTC 2 cut(s) 17, 105
BstMBI GATC 7 cut(s) 247, 327, 379, 415, 493, 512, 598
BstMWI GCNNNNNNNGC 4 cut(s) 364, 368, 434, 589
BstNI CCWGG 1 cut(s) 194
BstSCI CCNGG 1 cut(s) 192
BstSFI CTRYAG 1 cut(s) 438
BstUI CGCG 1 cut(s) 135
BstV1I GCAGC 1 cut(s) 193
BstX2I RGATCY 2 cut(s) 247, 598
BstYI RGATCY 2 cut(s) 247, 598
BtsCI GGATG 1 cut(s) 694
BtuMI TCGCGA 1 cut(s) 135
Cac8I GCNNGC 4 cut(s) 7, 133, 360, 369
CciI TCATGA 1 cut(s) 574
CseI GACGC 1 cut(s) 83
Csp6I GTAC 3 cut(s) 238, 404, 535
CviAII CATG 8 cut(s) 61, 169, 218, 241, 491, 538, 575, 652
CviQI GTAC 3 cut(s) 238, 404, 535
DdeI CTNAG 2 cut(s) 289, 633
DpnI GATC 7 cut(s) 249, 329, 381, 417, 495, 514, 600
DpnII GATC 7 cut(s) 247, 327, 379, 415, 493, 512, 598
DraI TTTAAA 1 cut(s) 349
Eam1104I CTCTTC 3 cut(s) 15, 620, 675
EarI CTCTTC 3 cut(s) 15, 620, 675
EciI GGCGGA 1 cut(s) 53
Eco130I CCWWGG 1 cut(s) 525
Eco31I GGTCTC 1 cut(s) 105
EcoRII CCWGG 1 cut(s) 192
EcoT14I CCWWGG 1 cut(s) 525
ErhI CCWWGG 1 cut(s) 525
Esp3I CGTCTC 1 cut(s) 17
FaeI CATG 8 cut(s) 64, 172, 221, 244, 494, 541, 578, 655
FatI CATG 8 cut(s) 60, 168, 217, 240, 490, 537, 574, 651
Fnu4HI GCNGC 1 cut(s) 207
Fsp4HI GCNGC 1 cut(s) 207
FspBI CTAG 1 cut(s) 389
GluI GCNGC 1 cut(s) 207
GsaI CCCAGC 3 cut(s) 13, 92, 366
HgaI GACGC 1 cut(s) 83
Hin1II CATG 8 cut(s) 64, 172, 221, 244, 494, 541, 578, 655
HindIII AAGCTT 1 cut(s) 581
HinfI GANTC 2 cut(s) 164, 287
HphI GGTGA 3 cut(s) 40, 118, 239
Hpy188I TCNGA 4 cut(s) 163, 256, 517, 603
Hpy188III TCNNGA 3 cut(s) 134, 389, 575
HpyAV CCTTC 1 cut(s) 261
HpyCH4III ACNGT 2 cut(s) 28, 562
HpyCH4IV ACGT 1 cut(s) 96
HpyCH4V TGCA 3 cut(s) 172, 209, 428
HpyF10VI GCNNNNNNNGC 4 cut(s) 364, 368, 434, 589
HpyF3I CTNAG 2 cut(s) 289, 633
HpySE526I ACGT 1 cut(s) 96
Hsp92II CATG 8 cut(s) 64, 172, 221, 244, 494, 541, 578, 655
Kzo9I GATC 7 cut(s) 247, 327, 379, 415, 493, 512, 598
LmnI GCTCC 1 cut(s) 550
LpnPI CCDG 8 cut(s) 102, 179, 206, 276, 344, 348, 353, 603
Lsp1109I GCAGC 1 cut(s) 193
LweI GCATC 3 cut(s) 86, 400, 415
MaeI CTAG 1 cut(s) 389
MaeII ACGT 1 cut(s) 96
MalI GATC 7 cut(s) 249, 329, 381, 417, 495, 514, 600
MboI GATC 7 cut(s) 247, 327, 379, 415, 493, 512, 598
MboII GAAGA 5 cut(s) 32, 149, 374, 637, 692
MfeI CAATTG 1 cut(s) 429
MflI RGATCY 2 cut(s) 247, 598
MluCI AATT 4 cut(s) 174, 392, 429, 444
MlyI GAGTC 2 cut(s) 158, 281
MmeI TCCRAC 2 cut(s) 180, 186
MseI TTAA 1 cut(s) 348
MslI CAYNNNNRTG 1 cut(s) 573
MspR9I CCNGG 1 cut(s) 194
MunI CAATTG 1 cut(s) 429
MvaI CCWGG 1 cut(s) 194
MvnI CGCG 1 cut(s) 135
MwoI GCNNNNNNNGC 4 cut(s) 364, 368, 434, 589
NdeII GATC 7 cut(s) 247, 327, 379, 415, 493, 512, 598
NlaIII CATG 8 cut(s) 64, 172, 221, 244, 494, 541, 578, 655
NruI TCGCGA 1 cut(s) 135
PagI TCATGA 1 cut(s) 574
PcsI WCGNNNNNNNCGW 1 cut(s) 81
PkrI GCNGC 1 cut(s) 208
PleI GAGTC 2 cut(s) 158, 281
PpsI GAGTC 2 cut(s) 158, 281
Ppu21I YACGTR 1 cut(s) 97
PsiI TTATAA 2 cut(s) 234, 323
Psp6I CCWGG 1 cut(s) 192
PspFI CCCAGC 3 cut(s) 9, 88, 362
PspGI CCWGG 1 cut(s) 192
PsuI RGATCY 2 cut(s) 247, 598
RruI TCGCGA 1 cut(s) 135
RsaI GTAC 3 cut(s) 239, 405, 536
RsaNI GTAC 3 cut(s) 238, 404, 535
RseI CAYNNNNRTG 1 cut(s) 573
SaqAI TTAA 1 cut(s) 348
SatI GCNGC 1 cut(s) 207
Sau3AI GATC 7 cut(s) 247, 327, 379, 415, 493, 512, 598
SchI GAGTC 2 cut(s) 158, 281
ScrFI CCNGG 1 cut(s) 194
SfaNI GCATC 3 cut(s) 86, 400, 415
SfcI CTRYAG 1 cut(s) 438
SmiMI CAYNNNNRTG 1 cut(s) 573
SmlI CTYRAG 1 cut(s) 587
SmoI CTYRAG 1 cut(s) 587
Sse9I AATT 4 cut(s) 174, 392, 429, 444
SsiI CCGC 1 cut(s) 38
SspMI CTAG 1 cut(s) 389
StyD4I CCNGG 1 cut(s) 192
StyI CCWWGG 1 cut(s) 525
TaaI ACNGT 2 cut(s) 28, 562
TaiI ACGT 1 cut(s) 99
TasI AATT 4 cut(s) 174, 392, 429, 444
TatI WGTACW 3 cut(s) 237, 403, 534
Tru1I TTAA 1 cut(s) 348
Tru9I TTAA 1 cut(s) 348
TseI GCWGC 1 cut(s) 206
TspDTI ATGAA 6 cut(s) 77, 554, 563, 620, 640, 693
TspGWI ACGGA 1 cut(s) 637
XapI RAATTY 2 cut(s) 174, 392
XbaI TCTAGA 1 cut(s) 388
XcmI CCANNNNNNNNNTGG 1 cut(s) 96
XspI CTAG 1 cut(s) 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.