RLG00000005634

Metal dependent phosphohydrolases with conserved 'HD' motif.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0013001
Physical Location & Seq
Reverse (-)
471227 .. 473204
1978 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005634

Sequence Viewer

Length: 684 bp
ATGGCGAGCTGGGATTGGGAAGAGACGGTGAGAAAGGCGGAGGAGTTGGTCGAAAAATCCATGAAAGGCAATGACGCATCACACGACCCAGCCCACGTCTGGAGGGTCAGAGACCTCGCACTCTCTCTTGCTCGCGAAGAACAAGCCCTCTCCTCCAACTCCGACTCCATGCAAATTGTGGAACTTGCTGCACTCCTCCATGATATAGGTGATTATAAGTACTTGAGAGATCCTTCAGAGGACAAACTTGTTGAGCACTTTCTTATTGACTCGGGCATAGAGGAGAGCAAGAAAACAAAGATTATAAAGATCATCAATGGAATGGGGTTTAAAGATGAGCTGGCTGGGCTGACAAACAGTGATCTTCCTCTAGAATTTGGGGTTGTACAAGATGCTGATCGGCTTGATGCAATTGGTGCTATGGGAATTGCTCGTTGCTTTACTTTTGGAGGAAGTAGGAATAGAGTGCTACATGATCCTAACATTCAGCCACAATCTGATTTATCCAAGGAAAAGTACATGAAAAAAGAGGAGCAAACTACTGTGAATCACTTTCATGAGAAGCTTCTCAAGCTAAAGGATCTGATGAAAACAAAGGCTGGACGGAAGAGAGCTGAGAAAAGGCACAAGTTCATGGAGGAGTTTCTTGTAGAGTTCTATGAAGAGTGGGATGGGAGAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HD

Protein Analysis

228

Amino Acids

26.08

Weight (kDa)

5.57

Isoelectric Point (pI)

38.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HD PF01966 32 - 137 1.3e-08 HD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013001)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17330
fragaria_vesca FvH4_4g36970 FvH4_4g36970 FvH4_4g36970
malus_domestica MD13G1004800.v1.1
prunus_persica Prupe.1G347900_v2.0.a1
pyrus_communis pycom13g00420
rosa_chinensis RchiOBHm_Chr4g0446761
rosa_laevigata RLG00000005634
rosa_multiflora Rmu_sc0001753.1_g000032
rosa_roxburghii Rroxscaffold_5G00387080
rosa_rugosa Rorug04G0374400 Rorug04G0374400
rosa_samantha Rh4AG435800 Rh4BG444700 Rh4CG461900 Rh4DG443600
rosa_wichuraiana Rw4G037250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 216, 305
AccII CGCG 1 cut(s) 135
AciI CCGC 1 cut(s) 38
AclWI GGATC 3 cut(s) 224, 470, 588
AcsI RAATTY 1 cut(s) 374
AcuI CTGAAG 1 cut(s) 219
AfaI GTAC 3 cut(s) 221, 387, 518
AfiI CCNNNNNNNGG 1 cut(s) 99
AjiI CACGTC 1 cut(s) 97
AluBI AGCT 6 cut(s) 9, 340, 565, 574, 614, 680
AluI AGCT 6 cut(s) 9, 340, 565, 574, 614, 680
Alw21I GWGCWC 1 cut(s) 258
Alw26I GTCTC 2 cut(s) 17, 105
AlwI GGATC 3 cut(s) 224, 470, 588
Ama87I CYCGRG 1 cut(s) 271
ApeKI GCWGC 1 cut(s) 188
ApoI RAATTY 1 cut(s) 374
AsuHPI GGTGA 2 cut(s) 40, 221
AvaI CYCGRG 1 cut(s) 271
Bbv12I GWGCWC 1 cut(s) 258
BbvI GCAGC 1 cut(s) 175
BccI CCATC 1 cut(s) 665
BcoDI GTCTC 2 cut(s) 17, 105
BfaI CTAG 1 cut(s) 371
BisI GCNGC 1 cut(s) 189
BlsI GCNGC 1 cut(s) 190
BmcAI AGTACT 1 cut(s) 221
BmeT110I CYCGRG 1 cut(s) 271
BmgBI CACGTC 1 cut(s) 97
BmsI GCATC 3 cut(s) 86, 382, 397
BpmI CTGGAG 1 cut(s) 121
BpuEI CTTGAG 2 cut(s) 244, 554
BsaBI GATNNNNATC 1 cut(s) 396
BsaI GGTCTC 1 cut(s) 105
BsaJI CCNNGG 1 cut(s) 507
BsaXI ACNNNNNCTCC 2 cut(s) 149, 179
Bsc4I CCNNNNNNNGG 1 cut(s) 99
Bse3DI GCAATG 1 cut(s) 76
Bse8I GATNNNNATC 1 cut(s) 396
BseDI CCNNGG 1 cut(s) 507
BseGI GGATG 1 cut(s) 676
BseJI GATNNNNATC 1 cut(s) 396
BseLI CCNNNNNNNGG 1 cut(s) 99
BseMI GCAATG 1 cut(s) 76
BseMII CTCAG 1 cut(s) 606
BseRI GAGGAG 6 cut(s) 56, 142, 185, 296, 545, 653
BseXI GCAGC 1 cut(s) 175
BseYI CCCAGC 3 cut(s) 9, 88, 344
BsgI GTGCAG 1 cut(s) 174
Bsh1236I CGCG 1 cut(s) 135
BsiHKAI GWGCWC 1 cut(s) 258
BsiHKCI CYCGRG 1 cut(s) 271
BslI CCNNNNNNNGG 1 cut(s) 99
BsmAI GTCTC 2 cut(s) 17, 105
BsmBI CGTCTC 1 cut(s) 17
Bso31I GGTCTC 1 cut(s) 105
BsoBI CYCGRG 1 cut(s) 271
Bsp1286I GDGCHC 1 cut(s) 258
Bsp1407I TGTACA 1 cut(s) 385
Bsp143I GATC 6 cut(s) 229, 309, 361, 397, 475, 580
Bsp68I TCGCGA 1 cut(s) 135
BspACI CCGC 1 cut(s) 38
BspCNI CTCAG 1 cut(s) 607
BspFNI CGCG 1 cut(s) 135
BspHI TCATGA 1 cut(s) 556
BspPI GGATC 3 cut(s) 224, 470, 588
BspTNI GGTCTC 1 cut(s) 105
BsrDI GCAATG 1 cut(s) 76
BsrGI TGTACA 1 cut(s) 385
BssECI CCNNGG 1 cut(s) 507
BssMI GATC 6 cut(s) 229, 309, 361, 397, 475, 580
BssT1I CCWWGG 1 cut(s) 507
Bst4CI ACNGT 3 cut(s) 28, 359, 544
Bst6I CTCTTC 3 cut(s) 15, 602, 657
BstAPI GCANNNNNTGC 1 cut(s) 416
BstAUI TGTACA 1 cut(s) 385
BstC8I GCNNGC 3 cut(s) 7, 133, 342
BstDEI CTNAG 1 cut(s) 615
BstF5I GGATG 1 cut(s) 676
BstFNI CGCG 1 cut(s) 135
BstKTI GATC 6 cut(s) 232, 312, 364, 400, 478, 583
BstMAI GTCTC 2 cut(s) 17, 105
BstMBI GATC 6 cut(s) 229, 309, 361, 397, 475, 580
BstMWI GCNNNNNNNGC 3 cut(s) 346, 416, 571
BstUI CGCG 1 cut(s) 135
BstV1I GCAGC 1 cut(s) 175
BstX2I RGATCY 2 cut(s) 229, 580
BstYI RGATCY 2 cut(s) 229, 580
BtrI CACGTC 1 cut(s) 97
BtsCI GGATG 1 cut(s) 676
BtsIMutI CAGTG 1 cut(s) 364
BtuMI TCGCGA 1 cut(s) 135
Cac8I GCNNGC 3 cut(s) 7, 133, 342
CciI TCATGA 1 cut(s) 556
CseI GACGC 1 cut(s) 83
Csp6I GTAC 3 cut(s) 220, 386, 517
CviAII CATG 7 cut(s) 61, 169, 200, 473, 520, 557, 634
CviQI GTAC 3 cut(s) 220, 386, 517
DdeI CTNAG 1 cut(s) 615
DpnI GATC 6 cut(s) 231, 311, 363, 399, 477, 582
DpnII GATC 6 cut(s) 229, 309, 361, 397, 475, 580
DraI TTTAAA 1 cut(s) 331
Eam1104I CTCTTC 3 cut(s) 15, 602, 657
EarI CTCTTC 3 cut(s) 15, 602, 657
EciI GGCGGA 1 cut(s) 53
Eco130I CCWWGG 1 cut(s) 507
Eco31I GGTCTC 1 cut(s) 105
Eco57I CTGAAG 1 cut(s) 219
Eco88I CYCGRG 1 cut(s) 271
EcoT14I CCWWGG 1 cut(s) 507
ErhI CCWWGG 1 cut(s) 507
Esp3I CGTCTC 1 cut(s) 17
FaeI CATG 7 cut(s) 64, 172, 203, 476, 523, 560, 637
FatI CATG 7 cut(s) 60, 168, 199, 472, 519, 556, 633
Fnu4HI GCNGC 1 cut(s) 189
Fsp4HI GCNGC 1 cut(s) 189
FspBI CTAG 1 cut(s) 371
GluI GCNGC 1 cut(s) 189
GsaI CCCAGC 3 cut(s) 13, 92, 348
GsuI CTGGAG 1 cut(s) 121
HgaI GACGC 1 cut(s) 83
Hin1II CATG 7 cut(s) 64, 172, 203, 476, 523, 560, 637
HindIII AAGCTT 1 cut(s) 563
HinfI GANTC 3 cut(s) 164, 269, 547
HphI GGTGA 2 cut(s) 40, 221
Hpy188I TCNGA 5 cut(s) 110, 163, 238, 499, 585
Hpy188III TCNNGA 4 cut(s) 100, 134, 371, 557
HpyAV CCTTC 1 cut(s) 243
HpyCH4III ACNGT 3 cut(s) 28, 359, 544
HpyCH4IV ACGT 1 cut(s) 96
HpyCH4V TGCA 3 cut(s) 172, 191, 410
HpyF10VI GCNNNNNNNGC 3 cut(s) 346, 416, 571
HpyF3I CTNAG 1 cut(s) 615
HpySE526I ACGT 1 cut(s) 96
Hsp92II CATG 7 cut(s) 64, 172, 203, 476, 523, 560, 637
Kzo9I GATC 6 cut(s) 229, 309, 361, 397, 475, 580
LmnI GCTCC 1 cut(s) 532
LpnPI CCDG 5 cut(s) 85, 102, 326, 330, 585
Lsp1109I GCAGC 1 cut(s) 175
LweI GCATC 3 cut(s) 86, 382, 397
MaeI CTAG 1 cut(s) 371
MaeII ACGT 1 cut(s) 96
MalI GATC 6 cut(s) 231, 311, 363, 399, 477, 582
MboI GATC 6 cut(s) 229, 309, 361, 397, 475, 580
MboII GAAGA 5 cut(s) 32, 149, 356, 619, 674
MfeI CAATTG 1 cut(s) 411
MflI RGATCY 2 cut(s) 229, 580
MhlI GDGCHC 1 cut(s) 258
MluCI AATT 4 cut(s) 174, 374, 411, 426
MlyI GAGTC 2 cut(s) 158, 263
MmeI TCCRAC 2 cut(s) 180, 186
MseI TTAA 1 cut(s) 330
MslI CAYNNNNRTG 1 cut(s) 555
MunI CAATTG 1 cut(s) 411
MvnI CGCG 1 cut(s) 135
MwoI GCNNNNNNNGC 3 cut(s) 346, 416, 571
NdeII GATC 6 cut(s) 229, 309, 361, 397, 475, 580
NlaIII CATG 7 cut(s) 64, 172, 203, 476, 523, 560, 637
NruI TCGCGA 1 cut(s) 135
PagI TCATGA 1 cut(s) 556
PcsI WCGNNNNNNNCGW 1 cut(s) 81
PfeI GAWTC 1 cut(s) 547
PkrI GCNGC 1 cut(s) 190
PleI GAGTC 2 cut(s) 158, 263
PpsI GAGTC 2 cut(s) 158, 263
PsiI TTATAA 2 cut(s) 216, 305
PspFI CCCAGC 3 cut(s) 9, 88, 344
PsuI RGATCY 2 cut(s) 229, 580
RruI TCGCGA 1 cut(s) 135
RsaI GTAC 3 cut(s) 221, 387, 518
RsaNI GTAC 3 cut(s) 220, 386, 517
RseI CAYNNNNRTG 1 cut(s) 555
SaqAI TTAA 1 cut(s) 330
SatI GCNGC 1 cut(s) 189
Sau3AI GATC 6 cut(s) 229, 309, 361, 397, 475, 580
ScaI AGTACT 1 cut(s) 221
SchI GAGTC 2 cut(s) 158, 263
SduI GDGCHC 1 cut(s) 258
SetI ASST 9 cut(s) 11, 99, 117, 211, 342, 567, 576, 616, 682
SfaNI GCATC 3 cut(s) 86, 382, 397
SmiMI CAYNNNNRTG 1 cut(s) 555
SmlI CTYRAG 2 cut(s) 223, 569
SmoI CTYRAG 2 cut(s) 223, 569
Sse9I AATT 4 cut(s) 174, 374, 411, 426
SsiI CCGC 1 cut(s) 38
SspMI CTAG 1 cut(s) 371
StyI CCWWGG 1 cut(s) 507
TaaI ACNGT 3 cut(s) 28, 359, 544
TaiI ACGT 1 cut(s) 99
TaqI TCGA 1 cut(s) 51
TasI AATT 4 cut(s) 174, 374, 411, 426
TatI WGTACW 3 cut(s) 219, 385, 516
TfiI GAWTC 1 cut(s) 547
Tru1I TTAA 1 cut(s) 330
Tru9I TTAA 1 cut(s) 330
TscAI CASTG 1 cut(s) 364
TseI GCWGC 1 cut(s) 188
TspDTI ATGAA 6 cut(s) 77, 536, 545, 602, 622, 675
TspGWI ACGGA 1 cut(s) 619
TspRI CASTG 1 cut(s) 364
XapI RAATTY 1 cut(s) 374
XbaI TCTAGA 1 cut(s) 370
XcmI CCANNNNNNNNNTGG 2 cut(s) 96, 175
XspI CTAG 1 cut(s) 371
ZrmI AGTACT 1 cut(s) 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.