Rh4BG444700

Metal dependent phosphohydrolases with conserved 'HD' motif.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0013001
Physical Location & Seq
Forward (+)
58424085 .. 58429153
5069 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG444700.1

Sequence Viewer

Length: 681 bp
ATGGCGAGCTGGAATTGGGAAGAGACGGTGAGAAAGGCGGAGGAGTTGGTCGTAAAATCCATGAAAGGCAATGACGCATCACACGACCCAGCCCACGTCTGGAGGGTCAGAGACCTCGCACTCTCTCTTGCTCGCGAAGAACAAGCCCTCTCCTCCAACTCCAACTCCGACTCCGACTCCATGCAAATTGTAGAACTTGCTGCACTCCTCCATGATATAGGTCAAGATCCTTCGGAGGACAAACTTGTTGAGCACTTTCTTATTGACTCGGGCATAGAGGAGAGCAAGAAAACAAAGATTATAAAGATCATCAATGGAATGGGGTTTAAAGATGAGCTGGCTGGGCTGACAAATAGTGATCTTCCTCTAGAATTTGGGGTTGCACAAGATGCTGATCGGCTTGATGCAATTGGTGCTATAGGAATTGCTCGTTGCTTTACTTTTGGAGGAAGTAGGAATAGAGTGCTACATGATCCTAACATTCAGCCACGATCTGGTTTATCCAAGGAAAAGTACATGAAAAAAGAGGAGCAAACTACCGTCAATCACTTTCATGAGAAGCTTCTCAAGCTAAAGGATCTGATGAAAACAAAGGCTGGATGGAAGAGAGCTGAGAAAACGCACAAGTTCATGGAGGAGTTTCTTGTAGAGTTCTATGAAGAGTGGGATGGGAGAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HD

Protein Analysis

226

Amino Acids

25.64

Weight (kDa)

5.55

Isoelectric Point (pI)

36.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HD PF01966 32 - 136 3.1e-09 HD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013001)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17330
fragaria_vesca FvH4_4g36970 FvH4_4g36970 FvH4_4g36970
malus_domestica MD13G1004800.v1.1
prunus_persica Prupe.1G347900_v2.0.a1
pyrus_communis pycom13g00420
rosa_chinensis RchiOBHm_Chr4g0446761
rosa_laevigata RLG00000005634
rosa_multiflora Rmu_sc0001753.1_g000032
rosa_roxburghii Rroxscaffold_5G00387080
rosa_rugosa Rorug04G0374400 Rorug04G0374400
rosa_samantha Rh4AG435800 Rh4BG444700 Rh4CG461900 Rh4DG443600
rosa_wichuraiana Rw4G037250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 302
AccB7I CCANNNNNTGG 1 cut(s) 494
AccII CGCG 1 cut(s) 135
AciI CCGC 1 cut(s) 38
AclWI GGATC 3 cut(s) 221, 467, 585
AcsI RAATTY 1 cut(s) 371
AfaI GTAC 1 cut(s) 515
AfiI CCNNNNNNNGG 2 cut(s) 99, 494
AjiI CACGTC 1 cut(s) 97
AluBI AGCT 6 cut(s) 9, 337, 562, 571, 611, 677
AluI AGCT 6 cut(s) 9, 337, 562, 571, 611, 677
Alw21I GWGCWC 1 cut(s) 255
Alw26I GTCTC 2 cut(s) 17, 105
AlwI GGATC 3 cut(s) 221, 467, 585
Ama87I CYCGRG 1 cut(s) 268
ApeKI GCWGC 1 cut(s) 200
ApoI RAATTY 1 cut(s) 371
AsuHPI GGTGA 1 cut(s) 40
AvaI CYCGRG 1 cut(s) 268
Bbv12I GWGCWC 1 cut(s) 255
BbvI GCAGC 1 cut(s) 187
BccI CCATC 2 cut(s) 594, 662
BcoDI GTCTC 2 cut(s) 17, 105
BfaI CTAG 1 cut(s) 368
BfmI CTRYAG 1 cut(s) 417
BisI GCNGC 1 cut(s) 201
BlsI GCNGC 1 cut(s) 202
BmeT110I CYCGRG 1 cut(s) 268
BmgBI CACGTC 1 cut(s) 97
BmsI GCATC 3 cut(s) 86, 379, 394
BpmI CTGGAG 1 cut(s) 121
BpuEI CTTGAG 1 cut(s) 551
BsaBI GATNNNNATC 1 cut(s) 393
BsaI GGTCTC 1 cut(s) 105
BsaJI CCNNGG 1 cut(s) 504
BsaXI ACNNNNNCTCC 6 cut(s) 149, 155, 161, 179, 185, 191
Bsc4I CCNNNNNNNGG 2 cut(s) 99, 494
Bse3DI GCAATG 1 cut(s) 76
Bse8I GATNNNNATC 1 cut(s) 393
BseDI CCNNGG 1 cut(s) 504
BseGI GGATG 2 cut(s) 605, 673
BseJI GATNNNNATC 1 cut(s) 393
BseLI CCNNNNNNNGG 2 cut(s) 99, 494
BseMI GCAATG 1 cut(s) 76
BseMII CTCAG 1 cut(s) 603
BseRI GAGGAG 6 cut(s) 56, 142, 197, 293, 542, 650
BseXI GCAGC 1 cut(s) 187
BseYI CCCAGC 2 cut(s) 88, 341
BsgI GTGCAG 1 cut(s) 186
Bsh1236I CGCG 1 cut(s) 135
BsiHKAI GWGCWC 1 cut(s) 255
BsiHKCI CYCGRG 1 cut(s) 268
BslI CCNNNNNNNGG 2 cut(s) 99, 494
BsmAI GTCTC 2 cut(s) 17, 105
BsmBI CGTCTC 1 cut(s) 17
Bso31I GGTCTC 1 cut(s) 105
BsoBI CYCGRG 1 cut(s) 268
Bsp1286I GDGCHC 1 cut(s) 255
Bsp143I GATC 7 cut(s) 226, 306, 358, 394, 472, 491, 577
Bsp68I TCGCGA 1 cut(s) 135
BspACI CCGC 1 cut(s) 38
BspCNI CTCAG 1 cut(s) 604
BspFNI CGCG 1 cut(s) 135
BspHI TCATGA 1 cut(s) 553
BspPI GGATC 3 cut(s) 221, 467, 585
BspTNI GGTCTC 1 cut(s) 105
BsrDI GCAATG 1 cut(s) 76
BssECI CCNNGG 1 cut(s) 504
BssMI GATC 7 cut(s) 226, 306, 358, 394, 472, 491, 577
BssT1I CCWWGG 1 cut(s) 504
Bst4CI ACNGT 2 cut(s) 28, 541
Bst6I CTCTTC 3 cut(s) 15, 599, 654
BstAPI GCANNNNNTGC 2 cut(s) 389, 413
BstC8I GCNNGC 3 cut(s) 7, 133, 339
BstDEI CTNAG 1 cut(s) 612
BstF5I GGATG 2 cut(s) 605, 673
BstFNI CGCG 1 cut(s) 135
BstKTI GATC 7 cut(s) 229, 309, 361, 397, 475, 494, 580
BstMAI GTCTC 2 cut(s) 17, 105
BstMBI GATC 7 cut(s) 226, 306, 358, 394, 472, 491, 577
BstMWI GCNNNNNNNGC 4 cut(s) 343, 389, 413, 568
BstSFI CTRYAG 1 cut(s) 417
BstUI CGCG 1 cut(s) 135
BstV1I GCAGC 1 cut(s) 187
BstX2I RGATCY 2 cut(s) 226, 577
BstYI RGATCY 2 cut(s) 226, 577
BtrI CACGTC 1 cut(s) 97
BtsCI GGATG 2 cut(s) 605, 673
BtuMI TCGCGA 1 cut(s) 135
Cac8I GCNNGC 3 cut(s) 7, 133, 339
CciI TCATGA 1 cut(s) 553
CseI GACGC 1 cut(s) 83
Csp6I GTAC 1 cut(s) 514
CviAII CATG 7 cut(s) 61, 181, 212, 470, 517, 554, 631
CviQI GTAC 1 cut(s) 514
DdeI CTNAG 1 cut(s) 612
DpnI GATC 7 cut(s) 228, 308, 360, 396, 474, 493, 579
DpnII GATC 7 cut(s) 226, 306, 358, 394, 472, 491, 577
DraI TTTAAA 1 cut(s) 328
Eam1104I CTCTTC 3 cut(s) 15, 599, 654
EarI CTCTTC 3 cut(s) 15, 599, 654
EciI GGCGGA 1 cut(s) 53
Eco130I CCWWGG 1 cut(s) 504
Eco31I GGTCTC 1 cut(s) 105
Eco88I CYCGRG 1 cut(s) 268
EcoT14I CCWWGG 1 cut(s) 504
ErhI CCWWGG 1 cut(s) 504
Esp3I CGTCTC 1 cut(s) 17
FaeI CATG 7 cut(s) 64, 184, 215, 473, 520, 557, 634
FatI CATG 7 cut(s) 60, 180, 211, 469, 516, 553, 630
Fnu4HI GCNGC 1 cut(s) 201
FokI GGATG 1 cut(s) 612
Fsp4HI GCNGC 1 cut(s) 201
FspBI CTAG 1 cut(s) 368
GluI GCNGC 1 cut(s) 201
GsaI CCCAGC 2 cut(s) 92, 345
GsuI CTGGAG 1 cut(s) 121
HgaI GACGC 1 cut(s) 83
Hin1II CATG 7 cut(s) 64, 184, 215, 473, 520, 557, 634
HindIII AAGCTT 1 cut(s) 560
HinfI GANTC 3 cut(s) 170, 176, 266
HphI GGTGA 1 cut(s) 40
Hpy188I TCNGA 5 cut(s) 110, 169, 175, 235, 582
Hpy188III TCNNGA 5 cut(s) 100, 134, 224, 368, 554
HpyAV CCTTC 1 cut(s) 240
HpyCH4III ACNGT 2 cut(s) 28, 541
HpyCH4IV ACGT 1 cut(s) 96
HpyCH4V TGCA 4 cut(s) 184, 203, 383, 407
HpyF10VI GCNNNNNNNGC 4 cut(s) 343, 389, 413, 568
HpyF3I CTNAG 1 cut(s) 612
HpySE526I ACGT 1 cut(s) 96
Hsp92II CATG 7 cut(s) 64, 184, 215, 473, 520, 557, 634
Kzo9I GATC 7 cut(s) 226, 306, 358, 394, 472, 491, 577
LmnI GCTCC 1 cut(s) 529
LpnPI CCDG 6 cut(s) 85, 102, 323, 327, 480, 582
Lsp1109I GCAGC 1 cut(s) 187
LweI GCATC 3 cut(s) 86, 379, 394
MaeI CTAG 1 cut(s) 368
MaeII ACGT 1 cut(s) 96
MalI GATC 7 cut(s) 228, 308, 360, 396, 474, 493, 579
MboI GATC 7 cut(s) 226, 306, 358, 394, 472, 491, 577
MboII GAAGA 5 cut(s) 32, 149, 353, 616, 671
MfeI CAATTG 1 cut(s) 408
MflI RGATCY 2 cut(s) 226, 577
MhlI GDGCHC 1 cut(s) 255
MluCI AATT 5 cut(s) 13, 186, 371, 408, 423
MlyI GAGTC 3 cut(s) 164, 170, 260
MmeI TCCRAC 4 cut(s) 180, 186, 192, 198
MseI TTAA 1 cut(s) 327
MslI CAYNNNNRTG 1 cut(s) 552
MunI CAATTG 1 cut(s) 408
MvnI CGCG 1 cut(s) 135
MwoI GCNNNNNNNGC 4 cut(s) 343, 389, 413, 568
NdeII GATC 7 cut(s) 226, 306, 358, 394, 472, 491, 577
NlaIII CATG 7 cut(s) 64, 184, 215, 473, 520, 557, 634
NruI TCGCGA 1 cut(s) 135
PagI TCATGA 1 cut(s) 553
PcsI WCGNNNNNNNCGW 1 cut(s) 81
PflMI CCANNNNNTGG 1 cut(s) 494
PkrI GCNGC 1 cut(s) 202
PleI GAGTC 3 cut(s) 164, 170, 260
PpsI GAGTC 3 cut(s) 164, 170, 260
PsiI TTATAA 1 cut(s) 302
PspFI CCCAGC 2 cut(s) 88, 341
PsuI RGATCY 2 cut(s) 226, 577
RruI TCGCGA 1 cut(s) 135
RsaI GTAC 1 cut(s) 515
RsaNI GTAC 1 cut(s) 514
RseI CAYNNNNRTG 1 cut(s) 552
SaqAI TTAA 1 cut(s) 327
SatI GCNGC 1 cut(s) 201
Sau3AI GATC 7 cut(s) 226, 306, 358, 394, 472, 491, 577
SchI GAGTC 3 cut(s) 164, 170, 260
SduI GDGCHC 1 cut(s) 255
SetI ASST 9 cut(s) 11, 99, 117, 223, 339, 564, 573, 613, 679
SfaNI GCATC 3 cut(s) 86, 379, 394
SfcI CTRYAG 1 cut(s) 417
SmiMI CAYNNNNRTG 1 cut(s) 552
SmlI CTYRAG 1 cut(s) 566
SmoI CTYRAG 1 cut(s) 566
Sse9I AATT 5 cut(s) 13, 186, 371, 408, 423
SsiI CCGC 1 cut(s) 38
SspMI CTAG 1 cut(s) 368
StyI CCWWGG 1 cut(s) 504
TaaI ACNGT 2 cut(s) 28, 541
TaiI ACGT 1 cut(s) 99
TasI AATT 5 cut(s) 13, 186, 371, 408, 423
TatI WGTACW 1 cut(s) 513
Tru1I TTAA 1 cut(s) 327
Tru9I TTAA 1 cut(s) 327
TseI GCWGC 1 cut(s) 200
TspDTI ATGAA 6 cut(s) 77, 533, 542, 599, 619, 672
Van91I CCANNNNNTGG 1 cut(s) 494
XapI RAATTY 1 cut(s) 371
XbaI TCTAGA 1 cut(s) 367
XcmI CCANNNNNNNNNTGG 1 cut(s) 96
XspI CTAG 1 cut(s) 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.