pycom13g00420

Metal dependent phosphohydrolases with conserved 'HD' motif.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0013001
Physical Location & Seq
Reverse (-)
264525 .. 267566
3042 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g00420.6

Sequence Viewer

Length: 930 bp
ATGATGAAGGAATGTGCTATCTCCACACCTCACTTTACCTCTGAGATAATTTATCACTTCATCCCGTCTTCACTCCATTATTCAGACCCTGTTTGTCTACAAATTTCACCTCCAACAACCGTTCACCCAGCCACGGTGGCGGCGGCGAGAGAGAAGAAAAGCAGGCAGTTGCAGACGATGGGGTCATTGGGATGGGAAGAGACGGTGAGAAAGGCGGAGAAGCTGGTGGAGGAGGCCATGAAGGAGAACGACGCGTCCCACGACGCTGCTCACGTGTGGCGGGTCCGAGACCTCGCTCTCTCTCTCGCCCGCGAAGAAGGCCTCTCTTCCAATTCCGACTCCATGCAAATTATAGAAGTGGCTGCACTTCTCCATGATATAGGTGATTACAAGTACTTGAGAGATCCCTCAGAGGAAAAAATTGTTGAGAACTTTCTCGATGAGGAGGGCATAGAGGAGACTAAGAAAATGAAGATTATAACGATCATCAAGGGAATGGGTTTCAAAGATGAGCTTGCAGGAACAAATAACGATTTTCCTCCAGAATTTGGCGTTGTTCAAGATGCTGATCGTCTTGATGCAATTGGTGCTATTGGAATTGCTCGTTGCTTTACTTTTGGTGGAAGTAGGAACAGAGTGCTACATGATCCTAAGATTCTGCCACGATGTGACTTATCTAAGGAAAATTACATGAAAAAGGATGAGCAGACTACTGTGAATCACTTTCACGAGAAGCTTCTTAAGCTAAAGGCTTTGATGAAAACAAAGAAGAGAGCTGAGAGAAGGCACAAGTTCATGGAGGAGTTTCTTGTAGAGTTCTATGAAGAGTGGGATGGGAGAAACTTATCAACTTTGCTCCTGGAGCGGAACGTGCTTGAAGATGCGGGCTATAATATTCAAACCAACTGTATTACAAAATATATTTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HD

Protein Analysis

310

Amino Acids

35.35

Weight (kDa)

5.73

Isoelectric Point (pI)

45.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013001)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17330
fragaria_vesca FvH4_4g36970 FvH4_4g36970 FvH4_4g36970
malus_domestica MD13G1004800.v1.1
prunus_persica Prupe.1G347900_v2.0.a1
pyrus_communis pycom13g00420
rosa_chinensis RchiOBHm_Chr4g0446761
rosa_laevigata RLG00000005634
rosa_multiflora Rmu_sc0001753.1_g000032
rosa_roxburghii Rroxscaffold_5G00387080
rosa_rugosa Rorug04G0374400 Rorug04G0374400
rosa_samantha Rh4AG435800 Rh4BG444700 Rh4CG461900 Rh4DG443600
rosa_wichuraiana Rw4G037250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 479, 928
AasI GACNNNNNNGTC 1 cut(s) 181
AccB7I CCANNNNNTGG 1 cut(s) 548
AccBSI CCGCTC 1 cut(s) 865
AccI GTMKAC 1 cut(s) 97
AccII CGCG 2 cut(s) 254, 312
AciI CCGC 7 cut(s) 140, 143, 215, 280, 310, 865, 884
AclWI GGATC 2 cut(s) 398, 641
AcsI RAATTY 2 cut(s) 102, 545
AcvI CACGTG 1 cut(s) 274
AdeI CACNNNGTG 1 cut(s) 668
AfaI GTAC 1 cut(s) 395
AfiI CCNNNNNNNGG 2 cut(s) 133, 548
AflII CTTAAG 1 cut(s) 740
AflIII ACRYGT 2 cut(s) 252, 273
AgsI TTSAA 4 cut(s) 505, 560, 878, 899
AjnI CCWGG 1 cut(s) 858
AloI GAACNNNNNNTCC 2 cut(s) 239, 271
AluBI AGCT 5 cut(s) 223, 514, 736, 745, 776
AluI AGCT 5 cut(s) 223, 514, 736, 745, 776
Alw26I GTCTC 3 cut(s) 194, 282, 452
AlwI GGATC 2 cut(s) 398, 641
AlwNI CAGNNNCTG 1 cut(s) 89
AoxI GGCC 2 cut(s) 234, 319
ApeKI GCWGC 2 cut(s) 266, 362
ApoI RAATTY 2 cut(s) 102, 545
AspS9I GGNCC 1 cut(s) 283
AsuHPI GGTGA 4 cut(s) 99, 116, 217, 395
AvaII GGWCC 1 cut(s) 283
BauI CACGAG 1 cut(s) 728
BbrPI CACGTG 1 cut(s) 274
BbsI GAAGAC 1 cut(s) 60
BbvI GCAGC 2 cut(s) 253, 349
BccI CCATC 3 cut(s) 172, 186, 827
BciT130I CCWGG 1 cut(s) 860
BcoDI GTCTC 3 cut(s) 194, 282, 452
BfrI CTTAAG 1 cut(s) 740
BglI GCCNNNNNGGC 1 cut(s) 137
BisI GCNGC 4 cut(s) 141, 144, 267, 363
BlsI GCNGC 4 cut(s) 142, 145, 268, 364
BmcAI AGTACT 1 cut(s) 395
Bme1390I CCNGG 1 cut(s) 860
Bme18I GGWCC 1 cut(s) 283
BmgT120I GGNCC 1 cut(s) 283
BmiI GGNNCC 1 cut(s) 284
BmrFI CCNGG 1 cut(s) 860
BmsI GCATC 3 cut(s) 553, 568, 871
BpiI GAAGAC 1 cut(s) 60
BpmI CTGGAG 2 cut(s) 525, 881
BpuEI CTTGAG 1 cut(s) 418
BsaAI YACGTR 1 cut(s) 274
BsaBI GATNNNNATC 1 cut(s) 567
BsaI GGTCTC 1 cut(s) 282
BsaJI CCNNGG 1 cut(s) 132
Bsc4I CCNNNNNNNGG 2 cut(s) 133, 548
Bse8I GATNNNNATC 1 cut(s) 567
BseBI CCWGG 1 cut(s) 860
BseDI CCNNGG 1 cut(s) 132
BseGI GGATG 4 cut(s) 60, 197, 706, 838
BseJI GATNNNNATC 1 cut(s) 567
BseLI CCNNNNNNNGG 2 cut(s) 133, 548
BseMII CTCAG 3 cut(s) 33, 423, 768
BseRI GAGGAG 4 cut(s) 245, 458, 470, 815
BseXI GCAGC 2 cut(s) 253, 349
BseYI CCCAGC 1 cut(s) 127
BsgI GTGCAG 1 cut(s) 348
Bsh1236I CGCG 2 cut(s) 254, 312
BshFI GGCC 2 cut(s) 236, 321
BslFI GGGAC 1 cut(s) 241
BslI CCNNNNNNNGG 2 cut(s) 133, 548
BsmAI GTCTC 3 cut(s) 194, 282, 452
BsmBI CGTCTC 1 cut(s) 194
BsmFI GGGAC 1 cut(s) 241
BsnI GGCC 2 cut(s) 236, 321
Bso31I GGTCTC 1 cut(s) 282
Bsp143I GATC 4 cut(s) 403, 483, 568, 646
BspACI CCGC 7 cut(s) 140, 143, 215, 280, 310, 865, 884
BspANI GGCC 2 cut(s) 236, 321
BspCNI CTCAG 3 cut(s) 34, 422, 769
BspFNI CGCG 2 cut(s) 254, 312
BspLI GGNNCC 1 cut(s) 284
BspPI GGATC 2 cut(s) 398, 641
BspTI CTTAAG 1 cut(s) 740
BspTNI GGTCTC 1 cut(s) 282
BsrBI CCGCTC 1 cut(s) 865
BssECI CCNNGG 1 cut(s) 132
BssMI GATC 4 cut(s) 403, 483, 568, 646
BssSI CACGAG 1 cut(s) 728
Bst2BI CACGAG 1 cut(s) 728
Bst2UI CCWGG 1 cut(s) 860
Bst4CI ACNGT 5 cut(s) 121, 136, 205, 715, 908
Bst6I CTCTTC 4 cut(s) 192, 331, 764, 819
BstAFI CTTAAG 1 cut(s) 740
BstAPI GCANNNNNTGC 1 cut(s) 587
BstBAI YACGTR 1 cut(s) 274
BstC8I GCNNGC 4 cut(s) 164, 310, 516, 886
BstDEI CTNAG 6 cut(s) 42, 409, 462, 651, 678, 777
BstDSI CCRYGG 1 cut(s) 132
BstF5I GGATG 4 cut(s) 60, 197, 706, 838
BstFNI CGCG 2 cut(s) 254, 312
BstKTI GATC 4 cut(s) 406, 486, 571, 649
BstMAI GTCTC 3 cut(s) 194, 282, 452
BstMBI GATC 4 cut(s) 403, 483, 568, 646
BstMWI GCNNNNNNNGC 6 cut(s) 137, 318, 587, 742, 862, 871
BstNI CCWGG 1 cut(s) 860
BstSCI CCNGG 1 cut(s) 858
BstUI CGCG 2 cut(s) 254, 312
BstV1I GCAGC 2 cut(s) 253, 349
BstV2I GAAGAC 1 cut(s) 60
BstX2I RGATCY 1 cut(s) 403
BstYI RGATCY 1 cut(s) 403
BsuRI GGCC 2 cut(s) 236, 321
BtgI CCRYGG 1 cut(s) 132
BtsCI GGATG 4 cut(s) 60, 197, 706, 838
Cac8I GCNNGC 4 cut(s) 164, 310, 516, 886
CaiI CAGNNNCTG 1 cut(s) 89
Cfr13I GGNCC 1 cut(s) 283
CseI GACGC 3 cut(s) 243, 260, 272
Csp6I GTAC 1 cut(s) 394
CviAII CATG 6 cut(s) 238, 343, 374, 644, 691, 796
CviQI GTAC 1 cut(s) 394
DdeI CTNAG 6 cut(s) 42, 409, 462, 651, 678, 777
DpnI GATC 4 cut(s) 405, 485, 570, 648
DpnII GATC 4 cut(s) 403, 483, 568, 646
DraIII CACNNNGTG 1 cut(s) 668
DrdI GACNNNNNNGTC 1 cut(s) 181
DseDI GACNNNNNNGTC 1 cut(s) 181
Eam1104I CTCTTC 4 cut(s) 192, 331, 764, 819
EarI CTCTTC 4 cut(s) 192, 331, 764, 819
EciI GGCGGA 1 cut(s) 230
Eco147I AGGCCT 1 cut(s) 321
Eco31I GGTCTC 1 cut(s) 282
Eco47I GGWCC 1 cut(s) 283
Eco72I CACGTG 1 cut(s) 274
EcoRII CCWGG 1 cut(s) 858
Esp3I CGTCTC 1 cut(s) 194
FaeI CATG 6 cut(s) 241, 346, 377, 647, 694, 799
FalI AAGNNNNNCTT 2 cut(s) 498, 530
FaqI GGGAC 1 cut(s) 241
FatI CATG 6 cut(s) 237, 342, 373, 643, 690, 795
FauI CCCGC 3 cut(s) 273, 317, 877
FblI GTMKAC 1 cut(s) 97
Fnu4HI GCNGC 4 cut(s) 141, 144, 267, 363
FokI GGATG 4 cut(s) 47, 204, 713, 845
Fsp4HI GCNGC 4 cut(s) 141, 144, 267, 363
GluI GCNGC 4 cut(s) 141, 144, 267, 363
GsaI CCCAGC 1 cut(s) 131
GsuI CTGGAG 2 cut(s) 525, 881
HaeIII GGCC 2 cut(s) 236, 321
HgaI GACGC 3 cut(s) 243, 260, 272
Hin1II CATG 6 cut(s) 241, 346, 377, 647, 694, 799
HindIII AAGCTT 1 cut(s) 734
HinfI GANTC 3 cut(s) 338, 655, 718
HphI GGTGA 4 cut(s) 99, 116, 217, 395
Hpy166II GTNNAC 2 cut(s) 98, 124
Hpy188I TCNGA 5 cut(s) 43, 85, 287, 337, 412
Hpy188III TCNNGA 5 cut(s) 437, 542, 560, 575, 728
Hpy8I GTNNAC 2 cut(s) 98, 124
Hpy99I CGWCG 2 cut(s) 254, 266
HpyAV CCTTC 3 cut(s) 235, 311, 777
HpyCH4III ACNGT 5 cut(s) 121, 136, 205, 715, 908
HpyCH4IV ACGT 2 cut(s) 273, 870
HpyCH4V TGCA 5 cut(s) 172, 346, 365, 518, 581
HpyF10VI GCNNNNNNNGC 6 cut(s) 137, 318, 587, 742, 862, 871
HpyF3I CTNAG 6 cut(s) 42, 409, 462, 651, 678, 777
HpySE526I ACGT 2 cut(s) 273, 870
Hsp92II CATG 6 cut(s) 241, 346, 377, 647, 694, 799
Kzo9I GATC 4 cut(s) 403, 483, 568, 646
LmnI GCTCC 2 cut(s) 861, 862
LpnPI CCDG 8 cut(s) 102, 141, 148, 209, 504, 555, 845, 872
Lsp1109I GCAGC 2 cut(s) 253, 349
LweI GCATC 3 cut(s) 553, 568, 871
MaeII ACGT 2 cut(s) 273, 870
MaeIII GTNAC 1 cut(s) 668
MalI GATC 4 cut(s) 405, 485, 570, 648
MbiI CCGCTC 1 cut(s) 865
MboI GATC 4 cut(s) 403, 483, 568, 646
MboII GAAGA 9 cut(s) 60, 166, 209, 318, 326, 484, 781, 836, 890
MfeI CAATTG 1 cut(s) 582
MflI RGATCY 1 cut(s) 403
MluCI AATT 9 cut(s) 48, 102, 331, 348, 420, 545, 582, 597, 685
MluI ACGCGT 1 cut(s) 252
MlyI GAGTC 1 cut(s) 332
MmeI TCCRAC 2 cut(s) 137, 360
MseI TTAA 1 cut(s) 741
MslI CAYNNNNRTG 1 cut(s) 190
MspCI CTTAAG 1 cut(s) 740
MspR9I CCNGG 1 cut(s) 860
MunI CAATTG 1 cut(s) 582
MvaI CCWGG 1 cut(s) 860
MvnI CGCG 2 cut(s) 254, 312
MwoI GCNNNNNNNGC 6 cut(s) 137, 318, 587, 742, 862, 871
NdeII GATC 4 cut(s) 403, 483, 568, 646
NlaIII CATG 6 cut(s) 241, 346, 377, 647, 694, 799
NlaIV GGNNCC 1 cut(s) 284
NmuCI GTSAC 1 cut(s) 668
PceI AGGCCT 1 cut(s) 321
PcsI WCGNNNNNNNCGW 2 cut(s) 258, 270
PfeI GAWTC 2 cut(s) 655, 718
PflMI CCANNNNNTGG 1 cut(s) 548
PfoI TCCNGGA 1 cut(s) 858
PkrI GCNGC 4 cut(s) 142, 145, 268, 364
PleI GAGTC 1 cut(s) 332
PmaCI CACGTG 1 cut(s) 274
PmlI CACGTG 1 cut(s) 274
PpsI GAGTC 1 cut(s) 332
Ppu21I YACGTR 1 cut(s) 274
PsiI TTATAA 2 cut(s) 479, 928
Psp6I CCWGG 1 cut(s) 858
PspCI CACGTG 1 cut(s) 274
PspFI CCCAGC 1 cut(s) 127
PspGI CCWGG 1 cut(s) 858
PspN4I GGNNCC 1 cut(s) 284
PspPI GGNCC 1 cut(s) 283
PstNI CAGNNNCTG 1 cut(s) 89
PsuI RGATCY 1 cut(s) 403
RsaI GTAC 1 cut(s) 395
RsaNI GTAC 1 cut(s) 394
RseI CAYNNNNRTG 1 cut(s) 190
SaqAI TTAA 1 cut(s) 741
SatI GCNGC 4 cut(s) 141, 144, 267, 363
Sau3AI GATC 4 cut(s) 403, 483, 568, 646
Sau96I GGNCC 1 cut(s) 283
ScaI AGTACT 1 cut(s) 395
SchI GAGTC 1 cut(s) 332
ScrFI CCNGG 1 cut(s) 860
SfaNI GCATC 3 cut(s) 553, 568, 871
SinI GGWCC 1 cut(s) 283
SmiMI CAYNNNNRTG 1 cut(s) 190
SmlI CTYRAG 2 cut(s) 397, 740
SmoI CTYRAG 2 cut(s) 397, 740
Sse9I AATT 9 cut(s) 48, 102, 331, 348, 420, 545, 582, 597, 685
SseBI AGGCCT 1 cut(s) 321
SsiI CCGC 7 cut(s) 140, 143, 215, 280, 310, 865, 884
SspI AATATT 1 cut(s) 895
StuI AGGCCT 1 cut(s) 321
StyD4I CCNGG 1 cut(s) 858
TaaI ACNGT 5 cut(s) 121, 136, 205, 715, 908
TaiI ACGT 2 cut(s) 276, 873
TaqI TCGA 1 cut(s) 438
TasI AATT 9 cut(s) 48, 102, 331, 348, 420, 545, 582, 597, 685
TatI WGTACW 1 cut(s) 393
TauI GCSGC 2 cut(s) 143, 146
TfiI GAWTC 2 cut(s) 655, 718
Tru1I TTAA 1 cut(s) 741
Tru9I TTAA 1 cut(s) 741
TseFI GTSAC 1 cut(s) 668
TseI GCWGC 2 cut(s) 266, 362
Tsp45I GTSAC 1 cut(s) 668
TspDTI ATGAA 8 cut(s) 20, 49, 254, 485, 707, 773, 784, 837
Van91I CCANNNNNTGG 1 cut(s) 548
Vha464I CTTAAG 1 cut(s) 740
VpaK11BI GGWCC 1 cut(s) 283
XapI RAATTY 2 cut(s) 102, 545
XmiI GTMKAC 1 cut(s) 97
ZrmI AGTACT 1 cut(s) 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.