MD13G1016000.v1.1

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0014915
Physical Location & Seq
Forward (+)
1023442 .. 1028285
4844 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1016000.v1.1.491

Sequence Viewer

Length: 1014 bp
ATGCTAGATATGGAAAACATGACCGTGATGAGGTCAAGTATTCTGTTCTCTGGTCTAATAATTCTATGCACCCTGTCATCTTTTACATCTTCTGCAGCGGAGCTACCACGATTCCCGCACGCAGCGAAAGCGGATGGGTCTATAAGCTTTTTGGTTCTGGGGGATTGGGGAAGAAGAGGAGCCTACAACCAATCTCAAGTTGCTAATCAGATGGGAATAATTGGAGAGAAGTTGGATATTGACTTTGTAATATCCACCGGCGATAATTTTTACGAAAATGGATTGACGGGCATCGATGATCCACAATTTGAAGACTCATTTAGCAAAATCTACACAGCACCAAGCTTGCAAAAGCAGTGGTACAGTGTTCTGGGTAACCATGATTATAGAGGGGATGTTGAGGCACAGTTGAGTCCTGTCCTTAGGGAATTGGATAGCAAATGGCTTTGCTTGAGATCCTTCATAGTTGATGCCGAAATTGTGGAAATCTTCTTTGTGGATACAACTCCCTTTGTTGATAAATACTTCACTGATCCGGAGGGCTCTGTCTATGACTGGAGTGGCATATTACCCCGAAATTATTACCTATCAAATCTTCTCAAGGATGTGGATTCAGCATTAAAAGATTCTACTGCAAAGTGGAAGATTGTGGTTGGTCACCACACGATTAGAACCGCTGGATATCATGGTGAAACGAAGGAGCTTGTAACACAGCTTCTCCCAATTCTTCTGGAAAACAATGTCGATCTTTACGCCAATGGCCACGACCATTGCTTAGAACACATAAGCAGTCCCAACAGCCCACTTCAATTTCTAACAAGTGGTGGTGGTTCAAAGGCATGGAGGGGTGTAGTTAACCCGTACAGCCCAAGCGAAATGAAGTTGTACTACGATGGGCAGGGTTTCATGTCAGTGCAAATCAATCAAACCGAATTGGATATTGCATTCTATGATGTTTTTGGCAATGTCTTGCACAAATGGGGGACATCAAAGCCGCACTACTCTTTCATGTAG

Protein Analysis

338

Amino Acids

37.85

Weight (kDa)

4.94

Isoelectric Point (pI)

38.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 49 - 258 4.2e-20 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014915)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g33011 FvH4_4g33011
malus_domestica MD13G1016000.v1.1 MD16G1013600.v1.1
prunus_persica Prupe.1G337600_v2.0.a1
pyrus_communis pycom13g01370
rosa_chinensis RchiOBHm_Chr4g0442051
rosa_laevigata RLG00000006048
rosa_multiflora Rmu_sc0002501.1_g000009
rosa_roxburghii Rroxscaffold_5G00382970
rosa_rugosa Rorug04G0338300
rosa_samantha Rh4AG390000 Rh4BG403400 Rh4CG418500 Rh4DG396600
rosa_wichuraiana Rw4G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 535
AciI CCGC 5 cut(s) 98, 116, 131, 675, 995
AclWI GGATC 3 cut(s) 293, 450, 527
AcoI YGGCCR 1 cut(s) 760
AfaI GTAC 3 cut(s) 362, 863, 887
AfiI CCNNNNNNNGG 1 cut(s) 30
AgsI TTSAA 3 cut(s) 311, 809, 834
AluBI AGCT 5 cut(s) 103, 147, 345, 703, 715
AluI AGCT 5 cut(s) 103, 147, 345, 703, 715
AlwI GGATC 3 cut(s) 293, 450, 527
Aor13HI TCCGGA 1 cut(s) 535
AoxI GGCC 1 cut(s) 760
ApeKI GCWGC 2 cut(s) 95, 122
AsuHPI GGTGA 2 cut(s) 650, 701
AxyI CCTNAGG 1 cut(s) 422
BalI TGGCCA 1 cut(s) 762
BanII GRGCYC 1 cut(s) 545
BarI GAAGNNNNNNTAC 4 cut(s) 699, 731, 872, 904
BbsI GAAGAC 1 cut(s) 318
BbvI GCAGC 2 cut(s) 107, 134
BccI CCATC 3 cut(s) 128, 205, 887
BciVI GTATCC 1 cut(s) 493
BfaI CTAG 1 cut(s) 5
BfmI CTRYAG 1 cut(s) 93
BfuI GTATCC 1 cut(s) 493
BisI GCNGC 3 cut(s) 96, 123, 995
BlsI GCNGC 3 cut(s) 97, 124, 996
BmiI GGNNCC 1 cut(s) 181
BmsI GCATC 2 cut(s) 300, 460
BpiI GAAGAC 1 cut(s) 318
BpmI CTGGAG 1 cut(s) 577
BpuEI CTTGAG 3 cut(s) 180, 472, 584
Bsa29I ATCGAT 1 cut(s) 294
BsaWI WCCGGW 1 cut(s) 535
BsaXI ACNNNNNCTCC 2 cut(s) 702, 732
Bsc4I CCNNNNNNNGG 1 cut(s) 30
Bse118I RCCGGY 1 cut(s) 257
Bse1I ACTGG 1 cut(s) 560
Bse21I CCTNAGG 1 cut(s) 422
Bse3DI GCAATG 2 cut(s) 769, 970
BseAI TCCGGA 1 cut(s) 535
BseCI ATCGAT 1 cut(s) 294
BseGI GGATG 3 cut(s) 139, 400, 610
BseLI CCNNNNNNNGG 1 cut(s) 30
BseMI GCAATG 2 cut(s) 769, 970
BseNI ACTGG 1 cut(s) 560
BseRI GAGGAG 1 cut(s) 192
BseXI GCAGC 2 cut(s) 107, 134
BshFI GGCC 1 cut(s) 762
BshVI ATCGAT 1 cut(s) 294
BsiSI CCGG 2 cut(s) 258, 536
BslFI GGGAC 2 cut(s) 777, 997
BslI CCNNNNNNNGG 1 cut(s) 30
BsmFI GGGAC 2 cut(s) 777, 997
BsmI GAATGC 1 cut(s) 944
BsnI GGCC 1 cut(s) 762
Bsp1286I GDGCHC 1 cut(s) 545
Bsp13I TCCGGA 1 cut(s) 535
Bsp143I GATC 4 cut(s) 298, 455, 532, 745
BspACI CCGC 5 cut(s) 98, 116, 131, 675, 995
BspANI GGCC 1 cut(s) 762
BspDI ATCGAT 1 cut(s) 294
BspEI TCCGGA 1 cut(s) 535
BspLI GGNNCC 1 cut(s) 181
BspMAI CTGCAG 1 cut(s) 97
BspPI GGATC 3 cut(s) 293, 450, 527
BsrDI GCAATG 2 cut(s) 769, 970
BsrFI RCCGGY 1 cut(s) 257
BsrI ACTGG 1 cut(s) 560
BssAI RCCGGY 1 cut(s) 257
BssMI GATC 4 cut(s) 298, 455, 532, 745
Bst4CI ACNGT 3 cut(s) 25, 365, 408
Bst6I CTCTTC 1 cut(s) 169
BstC8I GCNNGC 2 cut(s) 120, 347
BstDEI CTNAG 2 cut(s) 422, 775
BstEII GGTNACC 2 cut(s) 374, 656
BstF5I GGATG 3 cut(s) 139, 400, 610
BstKTI GATC 4 cut(s) 301, 458, 535, 748
BstMBI GATC 4 cut(s) 298, 455, 532, 745
BstMWI GCNNNNNNNGC 1 cut(s) 128
BstPI GGTNACC 2 cut(s) 374, 656
BstSFI CTRYAG 1 cut(s) 93
BstV1I GCAGC 2 cut(s) 107, 134
BstV2I GAAGAC 1 cut(s) 318
BstX2I RGATCY 1 cut(s) 455
BstYI RGATCY 1 cut(s) 455
Bsu15I ATCGAT 1 cut(s) 294
Bsu36I CCTNAGG 1 cut(s) 422
BsuI GTATCC 1 cut(s) 493
BsuRI GGCC 1 cut(s) 762
BsuTUI ATCGAT 1 cut(s) 294
BtsCI GGATG 3 cut(s) 139, 400, 610
BtsI GCAGTG 1 cut(s) 362
BtsIMutI CAGTG 4 cut(s) 362, 370, 528, 918
Cac8I GCNNGC 2 cut(s) 120, 347
Cfr10I RCCGGY 1 cut(s) 257
ClaI ATCGAT 1 cut(s) 294
Csp6I GTAC 3 cut(s) 361, 862, 886
CspCI CAANNNNNGTGG 4 cut(s) 338, 373, 752, 787
CviAII CATG 6 cut(s) 19, 380, 686, 840, 907, 1009
CviQI GTAC 3 cut(s) 361, 862, 886
DdeI CTNAG 2 cut(s) 422, 775
DpnI GATC 4 cut(s) 300, 457, 534, 747
DpnII GATC 4 cut(s) 298, 455, 532, 745
EaeI YGGCCR 1 cut(s) 760
Eam1104I CTCTTC 1 cut(s) 169
EarI CTCTTC 1 cut(s) 169
Eco24I GRGCYC 1 cut(s) 545
Eco32I GATATC 1 cut(s) 683
Eco81I CCTNAGG 1 cut(s) 422
Eco91I GGTNACC 2 cut(s) 374, 656
EcoO65I GGTNACC 2 cut(s) 374, 656
EcoRV GATATC 1 cut(s) 683
EcoT38I GRGCYC 1 cut(s) 545
FaeI CATG 6 cut(s) 22, 383, 689, 843, 910, 1012
FaqI GGGAC 2 cut(s) 777, 997
FatI CATG 6 cut(s) 18, 379, 685, 839, 906, 1008
FauI CCCGC 1 cut(s) 123
Fnu4HI GCNGC 3 cut(s) 96, 123, 995
FokI GGATG 3 cut(s) 146, 407, 617
FriOI GRGCYC 1 cut(s) 545
Fsp4HI GCNGC 3 cut(s) 96, 123, 995
FspBI CTAG 1 cut(s) 5
GluI GCNGC 3 cut(s) 96, 123, 995
GsuI CTGGAG 1 cut(s) 577
HaeIII GGCC 1 cut(s) 762
HapII CCGG 2 cut(s) 258, 536
Hin1II CATG 6 cut(s) 22, 383, 689, 843, 910, 1012
HincII GTYRAC 1 cut(s) 856
HindII GTYRAC 1 cut(s) 856
HindIII AAGCTT 2 cut(s) 145, 343
HinfI GANTC 5 cut(s) 111, 314, 412, 611, 626
HpaI GTTAAC 1 cut(s) 856
HpaII CCGG 2 cut(s) 258, 536
HphI GGTGA 2 cut(s) 650, 701
Hpy166II GTNNAC 1 cut(s) 856
Hpy188I TCNGA 1 cut(s) 210
Hpy188III TCNNGA 2 cut(s) 536, 731
Hpy8I GTNNAC 1 cut(s) 856
HpyAV CCTTC 2 cut(s) 469, 691
HpyCH4III ACNGT 3 cut(s) 25, 365, 408
HpyCH4V TGCA 7 cut(s) 69, 95, 349, 635, 916, 944, 973
HpyF10VI GCNNNNNNNGC 1 cut(s) 128
HpyF3I CTNAG 2 cut(s) 422, 775
Hsp92II CATG 6 cut(s) 22, 383, 689, 843, 910, 1012
Kpn2I TCCGGA 1 cut(s) 535
KspAI GTTAAC 1 cut(s) 856
Kzo9I GATC 4 cut(s) 298, 455, 532, 745
LmnI GCTCC 3 cut(s) 100, 179, 700
Lsp1109I GCAGC 2 cut(s) 107, 134
LweI GCATC 2 cut(s) 300, 460
MaeI CTAG 1 cut(s) 5
MaeIII GTNAC 3 cut(s) 374, 656, 706
MalI GATC 4 cut(s) 300, 457, 534, 747
MboI GATC 4 cut(s) 298, 455, 532, 745
MboII GAAGA 8 cut(s) 81, 183, 186, 323, 481, 587, 655, 719
MflI RGATCY 1 cut(s) 455
MhlI GDGCHC 1 cut(s) 545
MlsI TGGCCA 1 cut(s) 762
MluNI TGGCCA 1 cut(s) 762
MlyI GAGTC 2 cut(s) 308, 421
MmeI TCCRAC 1 cut(s) 213
MnlI CCTC 6 cut(s) 24, 170, 383, 394, 532, 837
Mox20I TGGCCA 1 cut(s) 762
MroI TCCGGA 1 cut(s) 535
MscI TGGCCA 1 cut(s) 762
MseI TTAA 2 cut(s) 620, 855
MslI CAYNNNNRTG 2 cut(s) 23, 911
Msp20I TGGCCA 1 cut(s) 762
MspA1I CMGCKG 2 cut(s) 98, 677
MspI CCGG 2 cut(s) 258, 536
Mva1269I GAATGC 1 cut(s) 944
MwoI GCNNNNNNNGC 1 cut(s) 128
NdeII GATC 4 cut(s) 298, 455, 532, 745
NlaIII CATG 6 cut(s) 22, 383, 689, 843, 910, 1012
NlaIV GGNNCC 1 cut(s) 181
NmuCI GTSAC 1 cut(s) 656
PctI GAATGC 1 cut(s) 944
PfeI GAWTC 3 cut(s) 111, 611, 626
PkrI GCNGC 3 cut(s) 97, 124, 996
PleI GAGTC 2 cut(s) 308, 420
PpsI GAGTC 2 cut(s) 308, 420
PspEI GGTNACC 2 cut(s) 374, 656
PspN4I GGNNCC 1 cut(s) 181
PstI CTGCAG 1 cut(s) 97
PsuI RGATCY 1 cut(s) 455
RsaI GTAC 3 cut(s) 362, 863, 887
RsaNI GTAC 3 cut(s) 361, 862, 886
RseI CAYNNNNRTG 2 cut(s) 23, 911
SaqAI TTAA 2 cut(s) 620, 855
SatI GCNGC 3 cut(s) 96, 123, 995
Sau3AI GATC 4 cut(s) 298, 455, 532, 745
SchI GAGTC 2 cut(s) 308, 421
SduI GDGCHC 1 cut(s) 545
SetI ASST 7 cut(s) 35, 105, 149, 347, 588, 705, 717
SfaNI GCATC 2 cut(s) 300, 460
SfcI CTRYAG 1 cut(s) 93
SgrAI CRCCGGYG 1 cut(s) 257
SmiMI CAYNNNNRTG 2 cut(s) 23, 911
SmlI CTYRAG 3 cut(s) 195, 451, 599
SmoI CTYRAG 3 cut(s) 195, 451, 599
SsiI CCGC 5 cut(s) 98, 116, 131, 675, 995
SspMI CTAG 1 cut(s) 5
TaaI ACNGT 3 cut(s) 25, 365, 408
TaqI TCGA 2 cut(s) 294, 744
TatI WGTACW 1 cut(s) 885
TauI GCSGC 1 cut(s) 997
TfiI GAWTC 3 cut(s) 111, 611, 626
Tru1I TTAA 2 cut(s) 620, 855
Tru9I TTAA 2 cut(s) 620, 855
TscAI CASTG 4 cut(s) 362, 370, 535, 918
TseFI GTSAC 1 cut(s) 656
TseI GCWGC 2 cut(s) 95, 122
Tsp45I GTSAC 1 cut(s) 656
TspDTI ATGAA 4 cut(s) 451, 893, 895, 997
TspRI CASTG 4 cut(s) 362, 370, 535, 918
XspI CTAG 1 cut(s) 5
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.