RLG00000006048

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0014915
Physical Location & Seq
Reverse (-)
3863668 .. 3865958
2291 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006048

Sequence Viewer

Length: 1017 bp
ATGAATGCAAGCCTTCGGTTCTTCTCTGCTCTGTTTCTTGTACTTGTAAGCTGCTGCAGCTTATCCTCTTGTGCAGGAGATGCAGATCATGAGCTACGACGGCTGCAACACGCTTCCTCCAAAGCCGACGGATCTCTGAGCTTCTTGGTCGTTGGAGATTGGGGAAGAAGAGGAACTTACAATCAATCCCGAGTTGCTCTTCAGATGGGAGTAGTTGGAGAGAAGTTGGATATCAATTTTGTAATATCCACCGGCGACAACTTTTACGATGATGGATTGACGGGCATCGACGATCCGGCCTTTGAAGAGTCGTTTTCCAAGATCTACACAGCACCTAGCTTGCAAAAGCAATGGTACAATGTTCTGGGTAATCATGATTATAGGGGTAATGTTGAGGCCCAATTAAGTCCTGTCCTGACTGGATTGGATGGCAGATGGCTTTGCATGAGATCCTTCATAGTTGATGCAGAAATTGCAGAGTTTTTCTTCGTGGACACAACTCCCTTTGCAGACAAATACTTCACTGACCCAGAGGACCATGTCTACGACTGGAGTGGCATATTACCCAGAAATGAGTACCTCTCAAATATTCTCAAGGATGTGGATTCAGCGCTGAAAGAATCGAACGCGAAGTGGAAGATCGTGGTCGGTCACCATGGAATTAGAAGTGCTGGACAACATGGTGACACTCCAGAGCTTGTATCAAAGCTTCTCCCAATTCTTGAGGCCAACGATGTTGATCTTTACATCAATGGGCACGACCATTGCTTACAACACATTAGTACTCCCAACAGCCCGATTCAGTTTCTAACGAGTGGTGGTGGATCGAAGGCATGGAGGGGCGTTGTAATGCCGTACGATCCGAGTGAAATAAAGCTGTATTACGACGGACAGGGTTTCATGTCAGTGCAGATCACTCCAAGCCAAGTAGATATTGCATTCTACGATGTTTTTGGTAATGTGTTGCACAAATGGGGCACATCAAAGGACCTTTACTCTACTACCATGTACATGTAG

Protein Analysis

339

Amino Acids

37.72

Weight (kDa)

4.98

Isoelectric Point (pI)

45.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 47 - 256 3.5e-19 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014915)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g33011 FvH4_4g33011
malus_domestica MD13G1016000.v1.1 MD16G1013600.v1.1
prunus_persica Prupe.1G337600_v2.0.a1
pyrus_communis pycom13g01370
rosa_chinensis RchiOBHm_Chr4g0442051
rosa_laevigata RLG00000006048
rosa_multiflora Rmu_sc0002501.1_g000009
rosa_roxburghii Rroxscaffold_5G00382970
rosa_rugosa Rorug04G0338300
rosa_samantha Rh4AG390000 Rh4BG403400 Rh4CG418500 Rh4DG396600
rosa_wichuraiana Rw4G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 543
AccII CGCG 1 cut(s) 629
AclWI GGATC 5 cut(s) 139, 287, 444, 832, 854
AcuI CTGAAG 1 cut(s) 185
AfaI GTAC 6 cut(s) 42, 356, 578, 784, 857, 1010
AfeI AGCGCT 1 cut(s) 612
AflIII ACRYGT 1 cut(s) 1011
AgsI TTSAA 1 cut(s) 305
AluBI AGCT 8 cut(s) 51, 60, 94, 141, 339, 697, 709, 877
AluI AGCT 8 cut(s) 51, 60, 94, 141, 339, 697, 709, 877
AlwI GGATC 5 cut(s) 139, 287, 444, 832, 854
Ama87I CYCGRG 1 cut(s) 189
Aor51HI AGCGCT 1 cut(s) 612
AoxI GGCC 3 cut(s) 297, 396, 726
ApeKI GCWGC 4 cut(s) 51, 54, 57, 103
AspLEI GCGC 1 cut(s) 613
AspS9I GGNCC 3 cut(s) 397, 535, 988
AsuHPI GGTGA 2 cut(s) 644, 695
AvaI CYCGRG 1 cut(s) 189
AvaII GGWCC 2 cut(s) 535, 988
BaeGI GKGCMC 2 cut(s) 759, 980
BarI GAAGNNNNNNTAC 2 cut(s) 693, 725
BbvI GCAGC 4 cut(s) 38, 41, 69, 90
BccI CCATC 4 cut(s) 199, 266, 422, 429
BceAI ACGGC 2 cut(s) 116, 838
BfaI CTAG 1 cut(s) 336
BfmI CTRYAG 1 cut(s) 55
BfoI RGCGCY 1 cut(s) 614
BglII AGATCT 1 cut(s) 321
BisI GCNGC 4 cut(s) 52, 55, 58, 104
BlsI GCNGC 4 cut(s) 53, 56, 59, 105
BmcAI AGTACT 1 cut(s) 784
Bme18I GGWCC 2 cut(s) 535, 988
BmeT110I CYCGRG 1 cut(s) 189
BmgT120I GGNCC 3 cut(s) 397, 535, 988
BmsI GCATC 3 cut(s) 70, 294, 454
BplI GAGNNNNNCTC 2 cut(s) 566, 598
BpmI CTGGAG 2 cut(s) 571, 675
BpuEI CTTGAG 2 cut(s) 578, 743
BsaBI GATNNNNATC 2 cut(s) 84, 738
BsaJI CCNNGG 1 cut(s) 655
BsaXI ACNNNNNCTCC 2 cut(s) 101, 131
Bse118I RCCGGY 1 cut(s) 251
Bse1I ACTGG 2 cut(s) 424, 554
Bse3DI GCAATG 2 cut(s) 356, 763
Bse8I GATNNNNATC 2 cut(s) 84, 738
BseDI CCNNGG 1 cut(s) 655
BseGI GGATG 2 cut(s) 433, 604
BseJI GATNNNNATC 2 cut(s) 84, 738
BseMI GCAATG 2 cut(s) 356, 763
BseMII CTCAG 1 cut(s) 128
BseNI ACTGG 2 cut(s) 424, 554
BseSI GKGCMC 2 cut(s) 759, 980
BseXI GCAGC 4 cut(s) 38, 41, 69, 90
BsgI GTGCAG 2 cut(s) 93, 929
Bsh1236I CGCG 1 cut(s) 629
BshFI GGCC 3 cut(s) 299, 398, 728
BsiHKCI CYCGRG 1 cut(s) 189
BsiSI CCGG 2 cut(s) 252, 296
BsiWI CGTACG 1 cut(s) 855
BsmI GAATGC 2 cut(s) 10, 938
BsnI GGCC 3 cut(s) 299, 398, 728
BsoBI CYCGRG 1 cut(s) 189
Bsp1286I GDGCHC 2 cut(s) 759, 980
Bsp1407I TGTACA 1 cut(s) 1008
Bsp19I CCATGG 1 cut(s) 655
BspANI GGCC 3 cut(s) 299, 398, 728
BspCNI CTCAG 1 cut(s) 129
BspFNI CGCG 1 cut(s) 629
BspHI TCATGA 2 cut(s) 88, 373
BspMAI CTGCAG 1 cut(s) 59
BspPI GGATC 5 cut(s) 139, 287, 444, 832, 854
BspQI GCTCTTC 1 cut(s) 204
BsrDI GCAATG 2 cut(s) 356, 763
BsrFI RCCGGY 1 cut(s) 251
BsrGI TGTACA 1 cut(s) 1008
BsrI ACTGG 2 cut(s) 424, 554
BssAI RCCGGY 1 cut(s) 251
BssECI CCNNGG 1 cut(s) 655
BssT1I CCWWGG 1 cut(s) 655
Bst6I CTCTTC 3 cut(s) 163, 204, 300
BstAPI GCANNNNNTGC 2 cut(s) 80, 473
BstAUI TGTACA 1 cut(s) 1008
BstC8I GCNNGC 2 cut(s) 10, 341
BstDEI CTNAG 1 cut(s) 137
BstDSI CCRYGG 1 cut(s) 655
BstEII GGTNACC 1 cut(s) 650
BstF5I GGATG 2 cut(s) 433, 604
BstFNI CGCG 1 cut(s) 629
BstH2I RGCGCY 1 cut(s) 614
BstHHI GCGC 1 cut(s) 613
BstMWI GCNNNNNNNGC 4 cut(s) 57, 80, 100, 473
BstNSI RCATGY 1 cut(s) 1015
BstPI GGTNACC 1 cut(s) 650
BstSFI CTRYAG 1 cut(s) 55
BstSLI GKGCMC 2 cut(s) 759, 980
BstUI CGCG 1 cut(s) 629
BstV1I GCAGC 4 cut(s) 38, 41, 69, 90
BstX2I RGATCY 3 cut(s) 131, 321, 449
BstYI RGATCY 3 cut(s) 131, 321, 449
BsuRI GGCC 3 cut(s) 299, 398, 728
BtgI CCRYGG 1 cut(s) 655
BtsCI GGATG 2 cut(s) 433, 604
BtsIMutI CAGTG 2 cut(s) 522, 912
Cac8I GCNNGC 2 cut(s) 10, 341
CciI TCATGA 2 cut(s) 88, 373
CfoI GCGC 1 cut(s) 613
Cfr10I RCCGGY 1 cut(s) 251
Cfr13I GGNCC 3 cut(s) 397, 535, 988
Csp6I GTAC 6 cut(s) 41, 355, 577, 783, 856, 1009
CviQI GTAC 6 cut(s) 41, 355, 577, 783, 856, 1009
DdeI CTNAG 1 cut(s) 137
Eam1104I CTCTTC 3 cut(s) 163, 204, 300
EarI CTCTTC 3 cut(s) 163, 204, 300
Eco130I CCWWGG 1 cut(s) 655
Eco32I GATATC 1 cut(s) 232
Eco47I GGWCC 2 cut(s) 535, 988
Eco47III AGCGCT 1 cut(s) 612
Eco57I CTGAAG 1 cut(s) 185
Eco88I CYCGRG 1 cut(s) 189
Eco91I GGTNACC 1 cut(s) 650
EcoO109I RGGNCCY 1 cut(s) 988
EcoO65I GGTNACC 1 cut(s) 650
EcoRV GATATC 1 cut(s) 232
EcoT14I CCWWGG 1 cut(s) 655
ErhI CCWWGG 1 cut(s) 655
FalI AAGNNNNNCTT 2 cut(s) 160, 192
FblI GTMKAC 1 cut(s) 543
Fnu4HI GCNGC 4 cut(s) 52, 55, 58, 104
FokI GGATG 2 cut(s) 440, 611
Fsp4HI GCNGC 4 cut(s) 52, 55, 58, 104
FspBI CTAG 1 cut(s) 336
GlaI GCGC 1 cut(s) 612
GluI GCNGC 4 cut(s) 52, 55, 58, 104
GsuI CTGGAG 2 cut(s) 571, 675
HaeII RGCGCY 1 cut(s) 614
HaeIII GGCC 3 cut(s) 299, 398, 728
HapII CCGG 2 cut(s) 252, 296
HhaI GCGC 1 cut(s) 613
Hin6I GCGC 1 cut(s) 611
HinP1I GCGC 1 cut(s) 611
HindIII AAGCTT 1 cut(s) 707
HinfI GANTC 4 cut(s) 308, 605, 620, 799
HpaII CCGG 2 cut(s) 252, 296
HphI GGTGA 2 cut(s) 644, 695
Hpy166II GTNNAC 2 cut(s) 493, 544
Hpy188I TCNGA 3 cut(s) 138, 204, 864
Hpy188III TCNNGA 6 cut(s) 89, 189, 374, 415, 692, 722
Hpy8I GTNNAC 2 cut(s) 493, 544
Hpy99I CGWCG 4 cut(s) 102, 131, 293, 890
HpyAV CCTTC 3 cut(s) 23, 463, 823
HpyF10VI GCNNNNNNNGC 4 cut(s) 57, 80, 100, 473
HpyF3I CTNAG 1 cut(s) 137
HspAI GCGC 1 cut(s) 611
LguI GCTCTTC 1 cut(s) 204
Lsp1109I GCAGC 4 cut(s) 38, 41, 69, 90
LweI GCATC 3 cut(s) 70, 294, 454
MaeI CTAG 1 cut(s) 336
MaeIII GTNAC 2 cut(s) 650, 683
MboII GAAGA 7 cut(s) 13, 177, 180, 191, 317, 478, 649
MflI RGATCY 3 cut(s) 131, 321, 449
MhlI GDGCHC 2 cut(s) 759, 980
MluCI AATT 5 cut(s) 235, 401, 471, 660, 717
MlyI GAGTC 1 cut(s) 317
MmeI TCCRAC 3 cut(s) 133, 196, 207
MnlI CCTC 8 cut(s) 76, 127, 164, 388, 526, 590, 718, 831
MseI TTAA 1 cut(s) 404
MslI CAYNNNNRTG 2 cut(s) 905, 1010
MspI CCGG 2 cut(s) 252, 296
Mva1269I GAATGC 2 cut(s) 10, 938
MvnI CGCG 1 cut(s) 629
MwoI GCNNNNNNNGC 4 cut(s) 57, 80, 100, 473
NcoI CCATGG 1 cut(s) 655
NmuCI GTSAC 2 cut(s) 650, 683
NspI RCATGY 1 cut(s) 1015
PagI TCATGA 2 cut(s) 88, 373
PciI ACATGT 1 cut(s) 1011
PciSI GCTCTTC 1 cut(s) 204
PctI GAATGC 2 cut(s) 10, 938
PfeI GAWTC 3 cut(s) 605, 620, 799
Pfl23II CGTACG 1 cut(s) 855
PflFI GACNNNGTC 1 cut(s) 539
PkrI GCNGC 4 cut(s) 53, 56, 59, 105
PleI GAGTC 1 cut(s) 316
PpsI GAGTC 1 cut(s) 316
PpuMI RGGWCCY 1 cut(s) 988
PscI ACATGT 1 cut(s) 1011
Psp5II RGGWCCY 1 cut(s) 988
PspEI GGTNACC 1 cut(s) 650
PspLI CGTACG 1 cut(s) 855
PspPI GGNCC 3 cut(s) 397, 535, 988
PspPPI RGGWCCY 1 cut(s) 988
PstI CTGCAG 1 cut(s) 59
PsuI RGATCY 3 cut(s) 131, 321, 449
PsyI GACNNNGTC 1 cut(s) 539
RsaI GTAC 6 cut(s) 42, 356, 578, 784, 857, 1010
RsaNI GTAC 6 cut(s) 41, 355, 577, 783, 856, 1009
RseI CAYNNNNRTG 2 cut(s) 905, 1010
SapI GCTCTTC 1 cut(s) 204
SaqAI TTAA 1 cut(s) 404
SatI GCNGC 4 cut(s) 52, 55, 58, 104
Sau96I GGNCC 3 cut(s) 397, 535, 988
ScaI AGTACT 1 cut(s) 784
SchI GAGTC 1 cut(s) 317
SduI GDGCHC 2 cut(s) 759, 980
SfaNI GCATC 3 cut(s) 70, 294, 454
SfcI CTRYAG 1 cut(s) 55
SgrAI CRCCGGYG 1 cut(s) 251
SinI GGWCC 2 cut(s) 535, 988
SmiMI CAYNNNNRTG 2 cut(s) 905, 1010
SmlI CTYRAG 2 cut(s) 593, 722
SmoI CTYRAG 2 cut(s) 593, 722
Sse9I AATT 5 cut(s) 235, 401, 471, 660, 717
SspI AATATT 1 cut(s) 589
SspMI CTAG 1 cut(s) 336
StyI CCWWGG 1 cut(s) 655
TaqI TCGA 3 cut(s) 288, 623, 827
TaqII GACCGA 1 cut(s) 638
TasI AATT 5 cut(s) 235, 401, 471, 660, 717
TatI WGTACW 3 cut(s) 40, 782, 1008
TfiI GAWTC 3 cut(s) 605, 620, 799
Tru1I TTAA 1 cut(s) 404
Tru9I TTAA 1 cut(s) 404
TscAI CASTG 2 cut(s) 529, 912
TseFI GTSAC 2 cut(s) 650, 683
TseI GCWGC 4 cut(s) 51, 54, 57, 103
Tsp45I GTSAC 2 cut(s) 650, 683
TspDTI ATGAA 3 cut(s) 17, 445, 889
TspGWI ACGGA 2 cut(s) 144, 903
TspRI CASTG 2 cut(s) 529, 912
Tth111I GACNNNGTC 1 cut(s) 539
VpaK11BI GGWCC 2 cut(s) 535, 988
XceI RCATGY 1 cut(s) 1015
XmiI GTMKAC 1 cut(s) 543
XspI CTAG 1 cut(s) 336
ZrmI AGTACT 1 cut(s) 784
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.